AT2G46494
ERF Family

BEST Arabidopsis thaliana protein match is Ring U-Box superfamily protein (TAIR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008440
Physical Location & Seq
Reverse (-)
19082344 .. 19083811
1468 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G46494.1

Sequence Viewer

Length: 1089 bp
ATGATAATCTCAAAACAACTTTTCCTTTTATTTTTCCTCCTCTTCTTCATCTTCCCTCTCCGACACGCCTCAAACCCTAGTAAATGTTCTTCTTCTAATTCGAGACCACACAGATGTGGACCCTTAGAAGTCCCCATCCGTTTCCCTTTCTGCGACCATCCGTTATTCAATCTCCTCTGCACCAATCTCAATAATACAGTCCTACAACTTCCTATGTCCGGAACATTTTTTGTCCAGTATATCGACTACAGAAAGCAACAGATTTATATCAACGATCCTGAAAACTGTTTGGCTAAAAGGCTTTTAACCTTCAACATTTCAGGATCCCCTTTCTCTCCTCGCTTTGATACCTTATACACGTTCCTGACTTGTCCTAATGAGCTCGTGTTGCCGTCTTGGTATCCGTCCATTCCTTGCCTAAGTAATTCCACCTCCTCCTTCTTCGCCACGTCTAATTTTGCTCTCGCAGAGTCTATGCTTCCTTCTTGTCAGATCGTGAAGAGAATATATGTTCCGGCTGATTCGCCGTTTGCAGAAACAAGATTCTCGAGCTACCTCAATCAAAGCCTCTTGTTGGAGTGGAACTCGCCTAATTGCAGAGGTTGTGAGATAGATTACTTGAGATGTGGATTCAAGAACAAAGCCTCCCCCGAAGTAAAATGCTTCGGTGCTAAAAAATCCGGTCATTTAAGCAGGGCAGTAGTAGCAGTACTTATATGTTTGTCCATAATAGGAGCAGTCATCCTCTTTGTTACTTGTATCGCTATCCGGATTCACAACACACCGAGACGACGACATTGGGCAGTACCAGCGGCAGCCGCAACCGTAATGCAGCAACCAAGAGAGGTTATGGCCACGAGGGGTCTTGACCAATCTACAATAGAAAAATACAAGACAATGGAGTTGGGAGAAAGTAGAAGGCCTCCCGGGACTAATGGTATTGTATGTCCCATTTGTTTATCAGAGTATGTGAGCAAAGAGACCGTACGGTTTATACCTGAATGTGACCATTGCTTCCACGCGAAATGTATAGATGTGTGGCTCAAGATTCATGGTTCATGTCCTCTTTGTCGGAACTCACGTGCGTGA

Protein Analysis

362

Amino Acids

40.92

Weight (kDa)

9.01

Isoelectric Point (pI)

57.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 38 - 92 3.8e-08 Wall-associated receptor kinase galacturonan-binding
zf-RING_2 PF13639 316 - 358 5.4e-13 Ring finger domain
zf-RING_11 PF17123 316 - 344 2e-07 RING-like zinc finger
zf-C3HC4_2 PF13923 316 - 357 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 316 - 358 2.2e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0008440)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28040 AT2G25410 AT2G46493 AT2G46494 AT2G46495 AT2G46495 AT5G36001 AT5G36001
fragaria_vesca FvH4_7g16350
malus_domestica MD07G1148900.v1.1
prunus_persica Prupe.2G187400_v2.0.a1
pyrus_communis pycom07g14790
rosa_chinensis RchiOBHm_Chr1g0359361
rosa_laevigata RLG00000027876
rosa_multiflora Rmu_ssc0000358.1_g000002
rosa_roxburghii Rroxscaffold_4G00297050
rosa_rugosa Rorug01G0270500
rosa_samantha Rh1AG283900 Rh1AG284000 Rh1BG250100 Rh1CG268000 Rh1DG279000
rosa_wichuraiana Rw1G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1022
AccIII TCCGGA 2 cut(s) 218, 768
AciI CCGC 2 cut(s) 812, 819
AclWI GGATC 3 cut(s) 269, 318, 331
AcoI YGGCCR 1 cut(s) 852
AcvI CACGTG 1 cut(s) 1082
AfaI GTAC 3 cut(s) 711, 807, 987
AfiI CCNNNNNNNGG 2 cut(s) 218, 574
AflIII ACRYGT 1 cut(s) 357
AgsI TTSAA 3 cut(s) 169, 313, 634
AjiI CACGTC 1 cut(s) 450
AleI CACNNNNGTG 2 cut(s) 114, 1084
AloI GAACNNNNNNTCC 2 cut(s) 629, 661
AluBI AGCT 2 cut(s) 382, 552
AluI AGCT 2 cut(s) 382, 552
Alw21I GWGCWC 1 cut(s) 384
Alw26I GTCTC 3 cut(s) 97, 781, 974
AlwI GGATC 3 cut(s) 269, 318, 331
Ama87I CYCGRG 2 cut(s) 547, 926
Aor13HI TCCGGA 2 cut(s) 218, 768
AoxI GGCC 2 cut(s) 852, 920
ApeKI GCWGC 2 cut(s) 815, 832
AspS9I GGNCC 1 cut(s) 119
AsuC2I CCSGG 2 cut(s) 927, 928
AvaI CYCGRG 2 cut(s) 547, 926
AvaII GGWCC 1 cut(s) 119
BalI TGGCCA 1 cut(s) 854
BamHI GGATCC 1 cut(s) 323
BanII GRGCYC 1 cut(s) 384
BarI GAAGNNNNNNTAC 2 cut(s) 73, 105
BauI CACGAG 2 cut(s) 383, 856
BbrPI CACGTG 1 cut(s) 1082
Bbv12I GWGCWC 1 cut(s) 384
BbvI GCAGC 2 cut(s) 827, 844
BccI CCATC 2 cut(s) 143, 165
BceAI ACGGC 2 cut(s) 376, 511
BciVI GTATCC 1 cut(s) 411
BcnI CCSGG 2 cut(s) 927, 928
BcoDI GTCTC 3 cut(s) 97, 781, 974
BfaI CTAG 1 cut(s) 78
BfmI CTRYAG 1 cut(s) 247
BfuI GTATCC 1 cut(s) 411
BisI GCNGC 4 cut(s) 813, 816, 819, 833
BlsI GCNGC 4 cut(s) 814, 817, 820, 834
BmcAI AGTACT 1 cut(s) 711
Bme1390I CCNGG 2 cut(s) 927, 928
Bme18I GGWCC 1 cut(s) 119
BmeT110I CYCGRG 2 cut(s) 547, 926
BmgBI CACGTC 1 cut(s) 450
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 2 cut(s) 121, 325
BmrFI CCNGG 2 cut(s) 927, 928
BplI GAGNNNNNCTC 2 cut(s) 569, 601
BpuEI CTTGAG 2 cut(s) 640, 1028
BpuMI CCSGG 2 cut(s) 927, 928
BsaAI YACGTR 1 cut(s) 1082
BsaBI GATNNNNATC 1 cut(s) 266
BsaI GGTCTC 2 cut(s) 97, 974
BsaJI CCNNGG 1 cut(s) 926
BsaWI WCCGGW 3 cut(s) 218, 680, 768
BsaXI ACNNNNNCTCC 2 cut(s) 629, 659
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 574
Bse1I ACTGG 1 cut(s) 235
Bse3DI GCAATG 1 cut(s) 1009
Bse8I GATNNNNATC 1 cut(s) 266
BseAI TCCGGA 2 cut(s) 218, 768
BseDI CCNNGG 1 cut(s) 926
BseGI GGATG 3 cut(s) 135, 157, 741
BseJI GATNNNNATC 1 cut(s) 266
BseLI CCNNNNNNNGG 2 cut(s) 218, 574
BseMI GCAATG 1 cut(s) 1009
BseNI ACTGG 1 cut(s) 235
BseRI GAGGAG 4 cut(s) 29, 164, 327, 424
BseXI GCAGC 2 cut(s) 827, 844
BsgI GTGCAG 1 cut(s) 163
Bsh1236I CGCG 1 cut(s) 1022
BshFI GGCC 2 cut(s) 854, 922
BsiHKAI GWGCWC 1 cut(s) 384
BsiHKCI CYCGRG 2 cut(s) 547, 926
BsiSI CCGG 5 cut(s) 219, 515, 681, 769, 927
BsiWI CGTACG 1 cut(s) 985
BslFI GGGAC 3 cut(s) 116, 933, 943
BslI CCNNNNNNNGG 2 cut(s) 218, 574
BsmAI GTCTC 3 cut(s) 97, 781, 974
BsmBI CGTCTC 1 cut(s) 781
BsmFI GGGAC 3 cut(s) 116, 933, 943
BsnI GGCC 2 cut(s) 854, 922
Bso31I GGTCTC 2 cut(s) 97, 974
BsoBI CYCGRG 2 cut(s) 547, 926
Bsp1286I GDGCHC 1 cut(s) 384
Bsp13I TCCGGA 2 cut(s) 218, 768
Bsp143I GATC 3 cut(s) 274, 323, 492
BspACI CCGC 2 cut(s) 812, 819
BspANI GGCC 2 cut(s) 854, 922
BspEI TCCGGA 2 cut(s) 218, 768
BspFNI CGCG 1 cut(s) 1022
BspLI GGNNCC 2 cut(s) 121, 325
BspPI GGATC 3 cut(s) 269, 318, 331
BspTNI GGTCTC 2 cut(s) 97, 974
BsrDI GCAATG 1 cut(s) 1009
BsrI ACTGG 1 cut(s) 235
BssECI CCNNGG 1 cut(s) 926
BssMI GATC 3 cut(s) 274, 323, 492
BssSI CACGAG 2 cut(s) 383, 856
Bst2BI CACGAG 2 cut(s) 383, 856
Bst4CI ACNGT 5 cut(s) 199, 287, 826, 985, 990
Bst6I CTCTTC 2 cut(s) 47, 494
BstBAI YACGTR 1 cut(s) 1082
BstDEI CTNAG 2 cut(s) 124, 419
BstF5I GGATG 3 cut(s) 135, 157, 741
BstFNI CGCG 1 cut(s) 1022
BstKTI GATC 3 cut(s) 277, 326, 495
BstMAI GTCTC 3 cut(s) 97, 781, 974
BstMBI GATC 3 cut(s) 274, 323, 492
BstMWI GCNNNNNNNGC 4 cut(s) 388, 704, 809, 818
BstSCI CCNGG 2 cut(s) 925, 926
BstSFI CTRYAG 1 cut(s) 247
BstUI CGCG 1 cut(s) 1022
BstV1I GCAGC 2 cut(s) 827, 844
BstX2I RGATCY 1 cut(s) 323
BstYI RGATCY 1 cut(s) 323
BsuI GTATCC 1 cut(s) 411
BsuRI GGCC 2 cut(s) 854, 922
BtrI CACGTC 1 cut(s) 450
BtsCI GGATG 3 cut(s) 135, 157, 741
Cfr13I GGNCC 1 cut(s) 119
Cfr9I CCCGGG 1 cut(s) 926
Csp6I GTAC 3 cut(s) 710, 806, 986
CviAII CATG 2 cut(s) 1052, 1059
CviQI GTAC 3 cut(s) 710, 806, 986
DdeI CTNAG 2 cut(s) 124, 419
DpnI GATC 3 cut(s) 276, 325, 494
DpnII GATC 3 cut(s) 274, 323, 492
EaeI YGGCCR 1 cut(s) 852
Eam1104I CTCTTC 2 cut(s) 47, 494
EarI CTCTTC 2 cut(s) 47, 494
Ecl136II GAGCTC 1 cut(s) 382
Eco147I AGGCCT 1 cut(s) 922
Eco24I GRGCYC 1 cut(s) 384
Eco31I GGTCTC 2 cut(s) 97, 974
Eco47I GGWCC 1 cut(s) 119
Eco53kI GAGCTC 1 cut(s) 382
Eco72I CACGTG 1 cut(s) 1082
Eco88I CYCGRG 2 cut(s) 547, 926
EcoICRI GAGCTC 1 cut(s) 382
EcoT38I GRGCYC 1 cut(s) 384
Esp3I CGTCTC 1 cut(s) 781
FaeI CATG 2 cut(s) 1055, 1062
FaqI GGGAC 3 cut(s) 116, 933, 943
FatI CATG 2 cut(s) 1051, 1058
Fnu4HI GCNGC 4 cut(s) 813, 816, 819, 833
FokI GGATG 3 cut(s) 122, 144, 728
FriOI GRGCYC 1 cut(s) 384
Fsp4HI GCNGC 4 cut(s) 813, 816, 819, 833
FspBI CTAG 1 cut(s) 78
GluI GCNGC 4 cut(s) 813, 816, 819, 833
HaeIII GGCC 2 cut(s) 854, 922
HapII CCGG 5 cut(s) 219, 515, 681, 769, 927
Hin1II CATG 2 cut(s) 1055, 1062
HinfI GANTC 6 cut(s) 470, 521, 543, 630, 772, 1048
HpaII CCGG 5 cut(s) 219, 515, 681, 769, 927
Hpy166II GTNNAC 1 cut(s) 119
Hpy188I TCNGA 4 cut(s) 62, 492, 964, 1074
Hpy8I GTNNAC 1 cut(s) 119
Hpy99I CGWCG 1 cut(s) 795
HpyAV CCTTC 4 cut(s) 319, 448, 492, 912
HpyCH4III ACNGT 5 cut(s) 199, 287, 826, 985, 990
HpyCH4IV ACGT 3 cut(s) 359, 449, 1081
HpyCH4V TGCA 4 cut(s) 180, 533, 597, 832
HpyF10VI GCNNNNNNNGC 4 cut(s) 388, 704, 809, 818
HpyF3I CTNAG 2 cut(s) 124, 419
HpySE526I ACGT 3 cut(s) 359, 449, 1081
Hsp92II CATG 2 cut(s) 1055, 1062
Kpn2I TCCGGA 2 cut(s) 218, 768
Kzo9I GATC 3 cut(s) 274, 323, 492
LmnI GCTCC 1 cut(s) 734
Lsp1109I GCAGC 2 cut(s) 827, 844
MaeI CTAG 1 cut(s) 78
MaeII ACGT 3 cut(s) 359, 449, 1081
MaeIII GTNAC 2 cut(s) 751, 1004
MalI GATC 3 cut(s) 276, 325, 494
MboI GATC 3 cut(s) 274, 323, 492
MboII GAAGA 7 cut(s) 34, 37, 43, 81, 84, 433, 511
MflI RGATCY 1 cut(s) 323
MhlI GDGCHC 1 cut(s) 384
MlsI TGGCCA 1 cut(s) 854
MluCI AATT 4 cut(s) 97, 424, 454, 592
MluNI TGGCCA 1 cut(s) 854
MlyI GAGTC 1 cut(s) 479
MmeI TCCRAC 3 cut(s) 85, 555, 1052
Mox20I TGGCCA 1 cut(s) 854
MroI TCCGGA 2 cut(s) 218, 768
MscI TGGCCA 1 cut(s) 854
MseI TTAA 2 cut(s) 305, 689
MslI CAYNNNNRTG 3 cut(s) 112, 114, 1084
Msp20I TGGCCA 1 cut(s) 854
MspA1I CMGCKG 1 cut(s) 812
MspI CCGG 5 cut(s) 219, 515, 681, 769, 927
MspR9I CCNGG 2 cut(s) 927, 928
MvnI CGCG 1 cut(s) 1022
MwoI GCNNNNNNNGC 4 cut(s) 388, 704, 809, 818
NciI CCSGG 2 cut(s) 927, 928
NdeII GATC 3 cut(s) 274, 323, 492
NlaIII CATG 2 cut(s) 1055, 1062
NlaIV GGNNCC 2 cut(s) 121, 325
NmuCI GTSAC 1 cut(s) 1004
OliI CACNNNNGTG 2 cut(s) 114, 1084
PaeR7I CTCGAG 1 cut(s) 547
PceI AGGCCT 1 cut(s) 922
PcsI WCGNNNNNNNCGW 1 cut(s) 1078
PfeI GAWTC 5 cut(s) 521, 543, 630, 772, 1048
Pfl23II CGTACG 1 cut(s) 985
PkrI GCNGC 4 cut(s) 814, 817, 820, 834
PleI GAGTC 1 cut(s) 478
PmaCI CACGTG 1 cut(s) 1082
PmlI CACGTG 1 cut(s) 1082
PpsI GAGTC 1 cut(s) 478
Ppu21I YACGTR 1 cut(s) 1082
Psp124BI GAGCTC 1 cut(s) 384
PspCI CACGTG 1 cut(s) 1082
PspLI CGTACG 1 cut(s) 985
PspN4I GGNNCC 2 cut(s) 121, 325
PspPI GGNCC 1 cut(s) 119
PsuI RGATCY 1 cut(s) 323
RsaI GTAC 3 cut(s) 711, 807, 987
RsaNI GTAC 3 cut(s) 710, 806, 986
RseI CAYNNNNRTG 3 cut(s) 112, 114, 1084
SacI GAGCTC 1 cut(s) 384
SaqAI TTAA 2 cut(s) 305, 689
SatI GCNGC 4 cut(s) 813, 816, 819, 833
Sau3AI GATC 3 cut(s) 274, 323, 492
Sau96I GGNCC 1 cut(s) 119
ScaI AGTACT 1 cut(s) 711
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 2 cut(s) 927, 928
SduI GDGCHC 1 cut(s) 384
SfcI CTRYAG 1 cut(s) 247
Sfr274I CTCGAG 1 cut(s) 547
SinI GGWCC 1 cut(s) 119
SlaI CTCGAG 1 cut(s) 547
SmaI CCCGGG 1 cut(s) 928
SmiMI CAYNNNNRTG 3 cut(s) 112, 114, 1084
SmlI CTYRAG 3 cut(s) 547, 619, 1043
SmoI CTYRAG 3 cut(s) 547, 619, 1043
Sse9I AATT 4 cut(s) 97, 424, 454, 592
SseBI AGGCCT 1 cut(s) 922
SsiI CCGC 2 cut(s) 812, 819
SspMI CTAG 1 cut(s) 78
SstI GAGCTC 1 cut(s) 384
StuI AGGCCT 1 cut(s) 922
StyD4I CCNGG 2 cut(s) 925, 926
TaaI ACNGT 5 cut(s) 199, 287, 826, 985, 990
TaiI ACGT 3 cut(s) 362, 452, 1084
TaqI TCGA 3 cut(s) 101, 243, 548
TasI AATT 4 cut(s) 97, 424, 454, 592
TatI WGTACW 1 cut(s) 709
TauI GCSGC 2 cut(s) 815, 821
TfiI GAWTC 5 cut(s) 521, 543, 630, 772, 1048
Tru1I TTAA 2 cut(s) 305, 689
Tru9I TTAA 2 cut(s) 305, 689
TseFI GTSAC 1 cut(s) 1004
TseI GCWGC 2 cut(s) 815, 832
Tsp45I GTSAC 1 cut(s) 1004
TspDTI ATGAA 3 cut(s) 37, 1040, 1047
TspGWI ACGGA 3 cut(s) 128, 150, 393
TspMI CCCGGG 1 cut(s) 926
VpaK11BI GGWCC 1 cut(s) 119
XhoI CTCGAG 1 cut(s) 547
XmaI CCCGGG 1 cut(s) 926
XspI CTAG 1 cut(s) 78
ZrmI AGTACT 1 cut(s) 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.