Rw1G025210

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0008440
Physical Location & Seq
Reverse (-)
51801429 .. 51803506
2078 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G025210.1

Sequence Viewer

Length: 1131 bp
ATGGACATCTCAGATATCCTCTTTCTCCTTCTTTTCCTATTTCCTCATTTCTATGCACTAGAATTAGACGACTGTCCAGCCTCCATGTGTGGCAACACCGGCTTTCCCATCCGGTTCCCTTTCGGGCTACAAGGCCATCAACCCAAAAACTGTTCTTACCCAGGTTTCGACCTAACCTGCAATAAACAGGGCATAACAGTCCTGAGACTTCCAAACTCTGGAGAATTTTTTGTCGGAGCAATCGATTATGTCACACAAGAGATTCAACTCTATGACCCTAGTAGTTGCCTTCCCAAAAGGCTTCTAACCCTCAACCTTTCAGGCTCTCCTTTTGTTGCTGCTTTTTATCAGAACTACACCTTCCTCAGCTGCCCTGCTTCATTCACAGAGTCAAAGTTCACCCCCATTGATTGCCTCAGTAACTCCACAACCTCAGTCTTGGCAACCCCTTCCATCAGCCTCGCAAATTCAATGTCTAAGGTGTGCAAGATCATCGCTACAATGCCAGTTCCGGTTTCTGGGATAGTTGAGTCTGAGGATGGATTCTCAACCAACTTTGATTCAGACCTTATCTTGACATGGTATGAGCCTGGTTGTACTCCTTGTGAAGGTGAAGGTGGCATTTGTGGCTTTGAGAGCAACACTAGCCAAGTACTTGGTTGCTTCCACAATTCTAAAGGCAGATCAGGCGACGGTTTCCAAGTTTTCAGGATTATTTGTGTCTCCATCGCGGTACCAGCCCTCACAATGGCGATTGGGATCGCTTGCTTTGCATGCGTCAAGGACAGGAGTGGGATCAGTCCTGGTCGCACTGCACAGCGGAACCCCACTGCAGTTGCAGCAGAACCCGAACAACAATCCACAATAGTAATGAATGGTCTGGATCAGACCACCATTGAATCCTATCAAAAGCTAGTTCTTGGTGAGAGCCGGCGGCTTCCAGGACCCAACGACACCACTTGCGCTATATGCTTATCAGAGTATCTCACCAAAGACACGGTAAGATGCATCCCAGAGTGTAAACACTGCTTCCATGCTGAATGTGTTGATGAGTGGCTGCGATTGAACAGTACATGTCCAGTTTGTCGAAACTCCCCCTCCCCAGCTCATGTCAACTCAGATGGGGTCTGA

Protein Analysis

376

Amino Acids

40.79

Weight (kDa)

4.94

Isoelectric Point (pI)

55.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 25 - 92 2.5e-17 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 154 - 224 1.4e-07 Wall-associated receptor kinase C-terminal
zf-RING_2 PF13639 320 - 363 5.3e-13 Ring finger domain
zf-RING_11 PF17123 321 - 349 1e-06 RING-like zinc finger
zf-C3HC4_2 PF13923 321 - 362 3.3e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008440)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28040 AT2G25410 AT2G46493 AT2G46494 AT2G46495 AT2G46495 AT5G36001 AT5G36001
fragaria_vesca FvH4_7g16350
malus_domestica MD07G1148900.v1.1
prunus_persica Prupe.2G187400_v2.0.a1
pyrus_communis pycom07g14790
rosa_chinensis RchiOBHm_Chr1g0359361
rosa_laevigata RLG00000027876
rosa_multiflora Rmu_ssc0000358.1_g000002
rosa_roxburghii Rroxscaffold_4G00297050
rosa_rugosa Rorug01G0270500
rosa_samantha Rh1AG283900 Rh1AG284000 Rh1BG250100 Rh1CG268000 Rh1DG279000
rosa_wichuraiana Rw1G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 185
Acc65I GGTACC 1 cut(s) 733
AccB1I GGYRCC 1 cut(s) 733
AccB7I CCANNNNNTGG 1 cut(s) 218
AccII CGCG 1 cut(s) 731
AciI CCGC 3 cut(s) 731, 820, 934
AclWI GGATC 3 cut(s) 767, 803, 891
AcsI RAATTY 2 cut(s) 224, 466
AfaI GTAC 4 cut(s) 598, 654, 735, 1072
AfiI CCNNNNNNNGG 4 cut(s) 218, 518, 608, 748
AflIII ACRYGT 1 cut(s) 1073
AgsI TTSAA 4 cut(s) 266, 471, 899, 1066
AjnI CCWGG 4 cut(s) 160, 589, 802, 940
AluBI AGCT 3 cut(s) 369, 913, 1106
AluI AGCT 3 cut(s) 369, 913, 1106
Alw26I GTCTC 2 cut(s) 199, 727
AlwI GGATC 3 cut(s) 767, 803, 891
AoxI GGCC 1 cut(s) 133
ApeKI GCWGC 4 cut(s) 338, 369, 839, 1057
ApoI RAATTY 2 cut(s) 224, 466
ArsI GACNNNNNNTTYG 2 cut(s) 458, 490
Asp718I GGTACC 1 cut(s) 733
AspLEI GCGC 1 cut(s) 965
AspS9I GGNCC 1 cut(s) 944
AsuHPI GGTGA 4 cut(s) 391, 623, 935, 979
AvaII GGWCC 1 cut(s) 944
BanI GGYRCC 1 cut(s) 733
BbvCI CCTCAGC 1 cut(s) 365
BbvI GCAGC 4 cut(s) 325, 356, 851, 1044
BccI CCATC 6 cut(s) 116, 144, 461, 533, 734, 1115
BcgI CGANNNNNNTGC 2 cut(s) 475, 509
BciT130I CCWGG 4 cut(s) 162, 591, 804, 942
BcoDI GTCTC 2 cut(s) 199, 727
BfaI CTAG 4 cut(s) 59, 279, 645, 914
BfmI CTRYAG 1 cut(s) 831
BfuAI ACCTGC 1 cut(s) 185
BisI GCNGC 5 cut(s) 339, 370, 840, 935, 1058
BlsI GCNGC 5 cut(s) 340, 371, 841, 936, 1059
BmcAI AGTACT 1 cut(s) 654
Bme1390I CCNGG 4 cut(s) 162, 591, 804, 942
Bme18I GGWCC 1 cut(s) 944
BmgT120I GGNCC 1 cut(s) 944
BmiI GGNNCC 4 cut(s) 116, 735, 824, 946
BmrFI CCNGG 4 cut(s) 162, 591, 804, 942
BmsI GCATC 2 cut(s) 995, 1017
BoxI GACNNNNGTC 1 cut(s) 72
BpmI CTGGAG 1 cut(s) 240
Bpu10I CCTNAGC 1 cut(s) 365
Bsa29I ATCGAT 1 cut(s) 243
BsaBI GATNNNNATC 1 cut(s) 758
BsaJI CCNNGG 1 cut(s) 160
BsaWI WCCGGW 2 cut(s) 111, 511
BsaXI ACNNNNNCTCC 2 cut(s) 1082, 1112
Bsc4I CCNNNNNNNGG 4 cut(s) 218, 518, 608, 748
Bse118I RCCGGY 2 cut(s) 98, 930
Bse1I ACTGG 2 cut(s) 506, 1079
Bse8I GATNNNNATC 1 cut(s) 758
BseBI CCWGG 4 cut(s) 162, 591, 804, 942
BseCI ATCGAT 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 3 cut(s) 108, 544, 1008
BseJI GATNNNNATC 1 cut(s) 758
BseLI CCNNNNNNNGG 4 cut(s) 218, 518, 608, 748
BseMII CTCAG 7 cut(s) 24, 194, 379, 430, 447, 525, 1131
BseNI ACTGG 2 cut(s) 506, 1079
BseXI GCAGC 4 cut(s) 325, 356, 851, 1044
BseYI CCCAGC 1 cut(s) 1102
BsgI GTGCAG 1 cut(s) 798
Bsh1236I CGCG 1 cut(s) 731
BshFI GGCC 1 cut(s) 135
BshNI GGYRCC 1 cut(s) 733
BshVI ATCGAT 1 cut(s) 243
BsiSI CCGG 4 cut(s) 99, 112, 512, 931
BslI CCNNNNNNNGG 4 cut(s) 218, 518, 608, 748
BsmAI GTCTC 2 cut(s) 199, 727
BsnI GGCC 1 cut(s) 135
Bsp143I GATC 5 cut(s) 489, 683, 759, 795, 883
BspACI CCGC 3 cut(s) 731, 820, 934
BspANI GGCC 1 cut(s) 135
BspCNI CTCAG 7 cut(s) 23, 195, 378, 429, 446, 526, 1130
BspDI ATCGAT 1 cut(s) 243
BspFNI CGCG 1 cut(s) 731
BspLI GGNNCC 4 cut(s) 116, 735, 824, 946
BspMAI CTGCAG 1 cut(s) 835
BspMI ACCTGC 1 cut(s) 185
BspPI GGATC 3 cut(s) 767, 803, 891
BspT107I GGYRCC 1 cut(s) 733
BsrFI RCCGGY 2 cut(s) 98, 930
BsrI ACTGG 2 cut(s) 506, 1079
BssAI RCCGGY 2 cut(s) 98, 930
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 5 cut(s) 489, 683, 759, 795, 883
Bst2UI CCWGG 4 cut(s) 162, 591, 804, 942
Bst4CI ACNGT 6 cut(s) 74, 152, 199, 695, 1000, 1070
BstC8I GCNNGC 3 cut(s) 766, 775, 932
BstDEI CTNAG 8 cut(s) 10, 203, 365, 416, 433, 477, 534, 1117
BstENI CCTNNNNNAGG 1 cut(s) 606
BstF5I GGATG 3 cut(s) 108, 544, 1008
BstFNI CGCG 1 cut(s) 731
BstHHI GCGC 1 cut(s) 965
BstKTI GATC 5 cut(s) 492, 686, 762, 798, 886
BstMAI GTCTC 2 cut(s) 199, 727
BstMBI GATC 5 cut(s) 489, 683, 759, 795, 883
BstNI CCWGG 4 cut(s) 162, 591, 804, 942
BstNSI RCATGY 2 cut(s) 777, 1077
BstPAI GACNNNNGTC 1 cut(s) 72
BstSCI CCNGG 4 cut(s) 160, 589, 802, 940
BstSFI CTRYAG 1 cut(s) 831
BstUI CGCG 1 cut(s) 731
BstV1I GCAGC 4 cut(s) 325, 356, 851, 1044
BstXI CCANNNNNNTGG 1 cut(s) 656
Bsu15I ATCGAT 1 cut(s) 243
BsuRI GGCC 1 cut(s) 135
BsuTUI ATCGAT 1 cut(s) 243
BtgZI GCGATG 2 cut(s) 478, 712
BtsCI GGATG 3 cut(s) 108, 544, 1008
BtsI GCAGTG 3 cut(s) 810, 828, 1024
BtsIMutI CAGTG 3 cut(s) 810, 828, 1024
BveI ACCTGC 1 cut(s) 185
Cac8I GCNNGC 3 cut(s) 766, 775, 932
CfoI GCGC 1 cut(s) 965
Cfr10I RCCGGY 2 cut(s) 98, 930
Cfr13I GGNCC 1 cut(s) 944
ClaI ATCGAT 1 cut(s) 243
CseI GACGC 1 cut(s) 766
Csp6I GTAC 4 cut(s) 597, 653, 734, 1071
CspCI CAANNNNNGTGG 2 cut(s) 817, 852
CviAII CATG 6 cut(s) 85, 579, 774, 1034, 1074, 1109
CviQI GTAC 4 cut(s) 597, 653, 734, 1071
DdeI CTNAG 8 cut(s) 10, 203, 365, 416, 433, 477, 534, 1117
DpnI GATC 5 cut(s) 491, 685, 761, 797, 885
DpnII GATC 5 cut(s) 489, 683, 759, 795, 883
Eco32I GATATC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 944
EcoNI CCTNNNNNAGG 1 cut(s) 606
EcoO109I RGGNCCY 1 cut(s) 944
EcoRII CCWGG 4 cut(s) 160, 589, 802, 940
EcoRV GATATC 1 cut(s) 16
EcoT22I ATGCAT 1 cut(s) 1010
FaeI CATG 6 cut(s) 88, 582, 777, 1037, 1077, 1112
FatI CATG 6 cut(s) 84, 578, 773, 1033, 1073, 1108
Fnu4HI GCNGC 5 cut(s) 339, 370, 840, 935, 1058
FokI GGATG 3 cut(s) 95, 551, 995
Fsp4HI GCNGC 5 cut(s) 339, 370, 840, 935, 1058
FspBI CTAG 4 cut(s) 59, 279, 645, 914
GlaI GCGC 1 cut(s) 964
GluI GCNGC 5 cut(s) 339, 370, 840, 935, 1058
GsaI CCCAGC 1 cut(s) 1106
GsuI CTGGAG 1 cut(s) 240
HaeIII GGCC 1 cut(s) 135
HapII CCGG 4 cut(s) 99, 112, 512, 931
HgaI GACGC 1 cut(s) 766
HhaI GCGC 1 cut(s) 965
Hin1II CATG 6 cut(s) 88, 582, 777, 1037, 1077, 1112
Hin6I GCGC 1 cut(s) 963
HinP1I GCGC 1 cut(s) 963
HincII GTYRAC 1 cut(s) 1114
HindII GTYRAC 1 cut(s) 1114
HinfI GANTC 6 cut(s) 262, 389, 530, 543, 560, 899
HpaII CCGG 4 cut(s) 99, 112, 512, 931
HphI GGTGA 4 cut(s) 391, 623, 935, 979
Hpy166II GTNNAC 3 cut(s) 399, 1022, 1114
Hpy188I TCNGA 9 cut(s) 13, 236, 351, 535, 565, 888, 979, 1120, 1130
Hpy188III TCNNGA 5 cut(s) 202, 219, 574, 709, 881
Hpy8I GTNNAC 3 cut(s) 399, 1022, 1114
Hpy99I CGWCG 1 cut(s) 695
HpyAV CCTTC 6 cut(s) 38, 299, 370, 459, 602, 608
HpyCH4III ACNGT 6 cut(s) 74, 152, 199, 695, 1000, 1070
HpyCH4V TGCA 8 cut(s) 56, 180, 486, 773, 815, 833, 839, 1008
HpyF3I CTNAG 8 cut(s) 10, 203, 365, 416, 433, 477, 534, 1117
Hsp92II CATG 6 cut(s) 88, 582, 777, 1037, 1077, 1112
HspAI GCGC 1 cut(s) 963
KpnI GGTACC 1 cut(s) 737
KroI GCCGGC 1 cut(s) 930
KroNI GCCGGC 1 cut(s) 932
Kzo9I GATC 5 cut(s) 489, 683, 759, 795, 883
LmnI GCTCC 1 cut(s) 236
Lsp1109I GCAGC 4 cut(s) 325, 356, 851, 1044
LweI GCATC 2 cut(s) 995, 1017
MaeI CTAG 4 cut(s) 59, 279, 645, 914
MaeIII GTNAC 2 cut(s) 250, 419
MalI GATC 5 cut(s) 491, 685, 761, 797, 885
MboI GATC 5 cut(s) 489, 683, 759, 795, 883
MluCI AATT 4 cut(s) 62, 224, 466, 670
MlyI GAGTC 2 cut(s) 398, 539
MmeI TCCRAC 1 cut(s) 214
Mph1103I ATGCAT 1 cut(s) 1010
MroNI GCCGGC 1 cut(s) 930
MslI CAYNNNNRTG 1 cut(s) 51
MspA1I CMGCKG 2 cut(s) 369, 820
MspI CCGG 4 cut(s) 99, 112, 512, 931
MspR9I CCNGG 4 cut(s) 162, 591, 804, 942
MvaI CCWGG 4 cut(s) 162, 591, 804, 942
MvnI CGCG 1 cut(s) 731
NaeI GCCGGC 1 cut(s) 932
NdeII GATC 5 cut(s) 489, 683, 759, 795, 883
NgoMIV GCCGGC 1 cut(s) 930
NlaIII CATG 6 cut(s) 88, 582, 777, 1037, 1077, 1112
NlaIV GGNNCC 4 cut(s) 116, 735, 824, 946
NmuCI GTSAC 1 cut(s) 250
NsiI ATGCAT 1 cut(s) 1010
NspI RCATGY 2 cut(s) 777, 1077
PaeI GCATGC 1 cut(s) 777
PciI ACATGT 1 cut(s) 1073
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PdiI GCCGGC 1 cut(s) 932
PfeI GAWTC 4 cut(s) 262, 543, 560, 899
PflMI CCANNNNNTGG 1 cut(s) 218
PfoI TCCNGGA 1 cut(s) 940
PkrI GCNGC 5 cut(s) 340, 371, 841, 936, 1059
PleI GAGTC 2 cut(s) 397, 538
PpsI GAGTC 2 cut(s) 397, 538
PpuMI RGGWCCY 1 cut(s) 944
PscI ACATGT 1 cut(s) 1073
PshAI GACNNNNGTC 1 cut(s) 72
Psp5II RGGWCCY 1 cut(s) 944
Psp6I CCWGG 4 cut(s) 160, 589, 802, 940
PspFI CCCAGC 1 cut(s) 1102
PspGI CCWGG 4 cut(s) 160, 589, 802, 940
PspN4I GGNNCC 4 cut(s) 116, 735, 824, 946
PspPI GGNCC 1 cut(s) 944
PspPPI RGGWCCY 1 cut(s) 944
PstI CTGCAG 1 cut(s) 835
PvuII CAGCTG 1 cut(s) 369
RsaI GTAC 4 cut(s) 598, 654, 735, 1072
RsaNI GTAC 4 cut(s) 597, 653, 734, 1071
RseI CAYNNNNRTG 1 cut(s) 51
SatI GCNGC 5 cut(s) 339, 370, 840, 935, 1058
Sau3AI GATC 5 cut(s) 489, 683, 759, 795, 883
Sau96I GGNCC 1 cut(s) 944
ScaI AGTACT 1 cut(s) 654
SchI GAGTC 2 cut(s) 398, 539
ScrFI CCNGG 4 cut(s) 162, 591, 804, 942
SfaNI GCATC 2 cut(s) 995, 1017
SfcI CTRYAG 1 cut(s) 831
SinI GGWCC 1 cut(s) 944
SmiMI CAYNNNNRTG 1 cut(s) 51
SphI GCATGC 1 cut(s) 777
Sse9I AATT 4 cut(s) 62, 224, 466, 670
SsiI CCGC 3 cut(s) 731, 820, 934
SspMI CTAG 4 cut(s) 59, 279, 645, 914
StyD4I CCNGG 4 cut(s) 160, 589, 802, 940
TaaI ACNGT 6 cut(s) 74, 152, 199, 695, 1000, 1070
TaqI TCGA 3 cut(s) 168, 243, 1087
TasI AATT 4 cut(s) 62, 224, 466, 670
TatI WGTACW 3 cut(s) 596, 652, 1070
TauI GCSGC 1 cut(s) 937
TfiI GAWTC 4 cut(s) 262, 543, 560, 899
TscAI CASTG 3 cut(s) 817, 835, 1031
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 4 cut(s) 338, 369, 839, 1057
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 2 cut(s) 369, 887
TspRI CASTG 3 cut(s) 817, 835, 1031
Van91I CCANNNNNTGG 1 cut(s) 218
VpaK11BI GGWCC 1 cut(s) 944
XagI CCTNNNNNAGG 1 cut(s) 606
XapI RAATTY 2 cut(s) 224, 466
XceI RCATGY 2 cut(s) 777, 1077
XspI CTAG 4 cut(s) 59, 279, 645, 914
ZrmI AGTACT 1 cut(s) 654
Zsp2I ATGCAT 1 cut(s) 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.