FvH4_7g16350

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0008440
Physical Location & Seq
Reverse (-)
14012229 .. 14015487
3259 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g16350.t1

Sequence Viewer

Length: 1119 bp
ATGGACATCTCAGACATCCTCTTCATCCTTCTCTTCCTGTTTCCTCATTTGTATGCACTAGCGTTAGGTGAGTGTCCAGCCTCCATGTGTGGCAACACCGGCTTTCCTATCCGATTCCCTTTCGGGCTACAAGGCTATCAACCGAAGAACTGTTCTTACCCAGGTTTCGACCTGACCTGCAACACACAGGGGACAACAGTCATTAGTCTTCCAAACTCAGGAGAGTTTTTTGTTCGAGCAATCGATTATGTGACACAGGAGATTAAACTCTATGAACCTAGTGGTTGCCTTCCCAGAAGGCTTCTGACCCTCGACCTGAAAGGCTCTCCTTTTGTTGCTTCTTTTTACCAGAACTACACCTTCCTCAGCTGCCCTGCTTCATTCACAAAGTCAAAGTTCGCCCCCATTGATTGCCTGAGTAACTCGACAACATCAGTCATGGCAACTCCTTCCACCAGCCTTGCGAATTCAATGTCCGCGACGTGCAAGATCATTGCTACAATGCCGGTTCCGGTTTCTGGGATAGTTCGGACTGAGGATGGATTCTCAGTCAACTTTGATTCAGATCTTAGCTTGACATGGTATGAGCCTGGTTGTTCTCCTTGTGAAGGTCAAGGTGGGACTTGTGGCTTCGAGAGCAACACTAGCCAAGTACTTGGTTGCTTCTACAACTCTACAGGCAGATCTTCCAACGGATTCCAAGTTTTCAAGATTATTTGCGTCTCCATCGTGGTACCGACCCTCACAATGGCAGTTGGGATAGCCTGCTTTGCTTGCCTCAAGGACAGGAGTCTTGATCGCACTGCACATCGGAACCCTGCAGTTGCAGCATTGCCTGAACAAGAATCCACAATTGTAATTCATGGTCTGGATCAGACCACCATTGAATCCTATCAAAAGCTAGTACTTGGTGAGAGCCGGCGACTTCCAGGACCTAACGACACCACTTGTGCTATATGCTTATCAGAGTATCTCATTAAAGACACAGTAAGATGCATCCCAGAGTGTAAACACTGCTTCCATGCTGAATGTGTTGATGAGTGGCTCAAATTGAACAGCACATGTCCAGTTTGTCGAAACAACCCCTCCCCAGCTCATGTCGACTCAAATGGAGTCTGA

Protein Analysis

373

Amino Acids

40.43

Weight (kDa)

5.4

Isoelectric Point (pI)

48.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 25 - 91 1e-17 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 149 - 224 9.8e-09 Wall-associated receptor kinase C-terminal
zf-RING_2 PF13639 316 - 359 1.2e-12 Ring finger domain
zf-RING_11 PF17123 317 - 345 2.1e-06 RING-like zinc finger
zf-C3HC4_2 PF13923 317 - 358 4.2e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0008440)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28040 AT2G25410 AT2G46493 AT2G46494 AT2G46495 AT2G46495 AT5G36001 AT5G36001
fragaria_vesca FvH4_7g16350
malus_domestica MD07G1148900.v1.1
prunus_persica Prupe.2G187400_v2.0.a1
pyrus_communis pycom07g14790
rosa_chinensis RchiOBHm_Chr1g0359361
rosa_laevigata RLG00000027876
rosa_multiflora Rmu_ssc0000358.1_g000002
rosa_roxburghii Rroxscaffold_4G00297050
rosa_rugosa Rorug01G0270500
rosa_samantha Rh1AG283900 Rh1AG284000 Rh1BG250100 Rh1CG268000 Rh1DG279000
rosa_wichuraiana Rw1G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 185
Acc65I GGTACC 1 cut(s) 733
AccB1I GGYRCC 1 cut(s) 733
AccI GTMKAC 1 cut(s) 1101
AccII CGCG 1 cut(s) 479
AciI CCGC 1 cut(s) 477
AclWI GGATC 1 cut(s) 879
AcsI RAATTY 1 cut(s) 466
AfaI GTAC 3 cut(s) 654, 735, 906
AfiI CCNNNNNNNGG 4 cut(s) 218, 518, 608, 748
AflIII ACRYGT 1 cut(s) 1061
AgsI TTSAA 4 cut(s) 471, 709, 887, 1054
AjiI CACGTC 1 cut(s) 483
AjnI CCWGG 3 cut(s) 160, 589, 928
AluBI AGCT 4 cut(s) 369, 573, 901, 1094
AluI AGCT 4 cut(s) 369, 573, 901, 1094
Alw26I GTCTC 1 cut(s) 727
AlwI GGATC 1 cut(s) 879
ApeKI GCWGC 2 cut(s) 369, 827
ApoI RAATTY 1 cut(s) 466
ArsI GACNNNNNNTTYG 2 cut(s) 458, 490
Asp718I GGTACC 1 cut(s) 733
AspS9I GGNCC 1 cut(s) 932
AsuHPI GGTGA 2 cut(s) 80, 923
AvaII GGWCC 1 cut(s) 932
BanI GGYRCC 1 cut(s) 733
BbsI GAAGAC 1 cut(s) 200
BbvCI CCTCAGC 1 cut(s) 365
BbvI GCAGC 2 cut(s) 356, 839
BccI CCATC 2 cut(s) 533, 734
BciT130I CCWGG 3 cut(s) 162, 591, 930
BcoDI GTCTC 1 cut(s) 727
BfaI CTAG 4 cut(s) 59, 279, 645, 902
BfmI CTRYAG 2 cut(s) 675, 819
BfuAI ACCTGC 1 cut(s) 185
BglII AGATCT 2 cut(s) 565, 683
BisI GCNGC 2 cut(s) 370, 828
BlsI GCNGC 2 cut(s) 371, 829
BmcAI AGTACT 2 cut(s) 654, 906
Bme1390I CCNGG 3 cut(s) 162, 591, 930
Bme18I GGWCC 1 cut(s) 932
BmgBI CACGTC 1 cut(s) 483
BmgT120I GGNCC 1 cut(s) 932
BmiI GGNNCC 3 cut(s) 510, 735, 815
BmrFI CCNGG 3 cut(s) 162, 591, 930
BmsI GCATC 2 cut(s) 983, 1005
BoxI GACNNNNGTC 2 cut(s) 197, 789
BpiI GAAGAC 1 cut(s) 200
Bpu10I CCTNAGC 1 cut(s) 365
BpuEI CTTGAG 1 cut(s) 764
Bsa29I ATCGAT 1 cut(s) 243
BsaBI GATNNNNATC 1 cut(s) 564
BsaJI CCNNGG 1 cut(s) 160
BsaWI WCCGGW 1 cut(s) 511
BsaXI ACNNNNNCTCC 2 cut(s) 1070, 1100
Bsc4I CCNNNNNNNGG 4 cut(s) 218, 518, 608, 748
Bse118I RCCGGY 3 cut(s) 98, 505, 918
Bse1I ACTGG 1 cut(s) 1067
Bse3DI GCAATG 2 cut(s) 492, 830
Bse8I GATNNNNATC 1 cut(s) 564
BseBI CCWGG 3 cut(s) 162, 591, 930
BseCI ATCGAT 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 4 cut(s) 15, 24, 544, 996
BseJI GATNNNNATC 1 cut(s) 564
BseLI CCNNNNNNNGG 4 cut(s) 218, 518, 608, 748
BseMI GCAATG 2 cut(s) 492, 830
BseMII CTCAG 6 cut(s) 24, 231, 379, 407, 525, 561
BseNI ACTGG 1 cut(s) 1067
BseXI GCAGC 2 cut(s) 356, 839
BseYI CCCAGC 1 cut(s) 1090
BsgI GTGCAG 1 cut(s) 789
Bsh1236I CGCG 1 cut(s) 479
BshNI GGYRCC 1 cut(s) 733
BshVI ATCGAT 1 cut(s) 243
BsiSI CCGG 4 cut(s) 99, 506, 512, 919
BslFI GGGAC 2 cut(s) 205, 634
BslI CCNNNNNNNGG 4 cut(s) 218, 518, 608, 748
BsmAI GTCTC 1 cut(s) 727
BsmBI CGTCTC 1 cut(s) 727
BsmFI GGGAC 2 cut(s) 205, 634
Bsp143I GATC 5 cut(s) 489, 565, 683, 796, 871
BspACI CCGC 1 cut(s) 477
BspCNI CTCAG 6 cut(s) 23, 230, 378, 408, 526, 560
BspDI ATCGAT 1 cut(s) 243
BspFNI CGCG 1 cut(s) 479
BspLI GGNNCC 3 cut(s) 510, 735, 815
BspMAI CTGCAG 1 cut(s) 823
BspMI ACCTGC 1 cut(s) 185
BspPI GGATC 1 cut(s) 879
BspT107I GGYRCC 1 cut(s) 733
BsrDI GCAATG 2 cut(s) 492, 830
BsrFI RCCGGY 3 cut(s) 98, 505, 918
BsrI ACTGG 1 cut(s) 1067
BssAI RCCGGY 3 cut(s) 98, 505, 918
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 5 cut(s) 489, 565, 683, 796, 871
Bst2UI CCWGG 3 cut(s) 162, 591, 930
Bst4CI ACNGT 3 cut(s) 152, 199, 988
Bst6I CTCTTC 2 cut(s) 26, 38
BstC8I GCNNGC 3 cut(s) 766, 775, 920
BstDEI CTNAG 7 cut(s) 10, 217, 365, 416, 534, 547, 569
BstENI CCTNNNNNAGG 1 cut(s) 606
BstF5I GGATG 4 cut(s) 15, 24, 544, 996
BstFNI CGCG 1 cut(s) 479
BstKTI GATC 5 cut(s) 492, 568, 686, 799, 874
BstMAI GTCTC 1 cut(s) 727
BstMBI GATC 5 cut(s) 489, 565, 683, 796, 871
BstMWI GCNNNNNNNGC 6 cut(s) 99, 636, 645, 770, 774, 827
BstNI CCWGG 3 cut(s) 162, 591, 930
BstNSI RCATGY 1 cut(s) 1065
BstPAI GACNNNNGTC 2 cut(s) 197, 789
BstSCI CCNGG 3 cut(s) 160, 589, 928
BstSFI CTRYAG 2 cut(s) 675, 819
BstUI CGCG 1 cut(s) 479
BstV1I GCAGC 2 cut(s) 356, 839
BstV2I GAAGAC 1 cut(s) 200
BstX2I RGATCY 2 cut(s) 565, 683
BstXI CCANNNNNNTGG 1 cut(s) 656
BstYI RGATCY 2 cut(s) 565, 683
Bsu15I ATCGAT 1 cut(s) 243
BsuTUI ATCGAT 1 cut(s) 243
BtrI CACGTC 1 cut(s) 483
BtsCI GGATG 4 cut(s) 15, 24, 544, 996
BtsI GCAGTG 2 cut(s) 801, 1012
BtsIMutI CAGTG 2 cut(s) 801, 1012
BveI ACCTGC 1 cut(s) 185
Cac8I GCNNGC 3 cut(s) 766, 775, 920
Cfr10I RCCGGY 3 cut(s) 98, 505, 918
Cfr13I GGNCC 1 cut(s) 932
ClaI ATCGAT 1 cut(s) 243
CseI GACGC 1 cut(s) 709
Csp6I GTAC 3 cut(s) 653, 734, 905
CspCI CAANNNNNGTGG 2 cut(s) 442, 477
CviAII CATG 7 cut(s) 85, 439, 579, 863, 1022, 1062, 1097
CviQI GTAC 3 cut(s) 653, 734, 905
DdeI CTNAG 7 cut(s) 10, 217, 365, 416, 534, 547, 569
DpnI GATC 5 cut(s) 491, 567, 685, 798, 873
DpnII GATC 5 cut(s) 489, 565, 683, 796, 871
Eam1104I CTCTTC 2 cut(s) 26, 38
EarI CTCTTC 2 cut(s) 26, 38
Eco47I GGWCC 1 cut(s) 932
EcoNI CCTNNNNNAGG 1 cut(s) 606
EcoO109I RGGNCCY 1 cut(s) 932
EcoRI GAATTC 1 cut(s) 466
EcoRII CCWGG 3 cut(s) 160, 589, 928
EcoT22I ATGCAT 1 cut(s) 998
Esp3I CGTCTC 1 cut(s) 727
FaeI CATG 7 cut(s) 88, 442, 582, 866, 1025, 1065, 1100
FaqI GGGAC 2 cut(s) 205, 634
FatI CATG 7 cut(s) 84, 438, 578, 862, 1021, 1061, 1096
FblI GTMKAC 1 cut(s) 1101
Fnu4HI GCNGC 2 cut(s) 370, 828
FokI GGATG 4 cut(s) 2, 11, 551, 983
Fsp4HI GCNGC 2 cut(s) 370, 828
FspBI CTAG 4 cut(s) 59, 279, 645, 902
GluI GCNGC 2 cut(s) 370, 828
GsaI CCCAGC 1 cut(s) 1094
HapII CCGG 4 cut(s) 99, 506, 512, 919
HgaI GACGC 1 cut(s) 709
Hin1II CATG 7 cut(s) 88, 442, 582, 866, 1025, 1065, 1100
HincII GTYRAC 2 cut(s) 553, 1102
HindII GTYRAC 2 cut(s) 553, 1102
HinfI GANTC 9 cut(s) 114, 543, 560, 696, 790, 845, 887, 1103, 1113
HpaII CCGG 4 cut(s) 99, 506, 512, 919
HphI GGTGA 2 cut(s) 80, 923
Hpy166II GTNNAC 3 cut(s) 553, 1010, 1102
Hpy188I TCNGA 9 cut(s) 13, 113, 306, 531, 565, 813, 876, 967, 1118
Hpy188III TCNNGA 5 cut(s) 219, 634, 709, 794, 869
Hpy8I GTNNAC 3 cut(s) 553, 1010, 1102
Hpy99I CGWCG 1 cut(s) 484
HpyAV CCTTC 6 cut(s) 38, 291, 299, 370, 459, 602
HpyCH4III ACNGT 3 cut(s) 152, 199, 988
HpyCH4IV ACGT 1 cut(s) 482
HpyCH4V TGCA 7 cut(s) 56, 180, 486, 806, 821, 827, 996
HpyF10VI GCNNNNNNNGC 6 cut(s) 99, 636, 645, 770, 774, 827
HpyF3I CTNAG 7 cut(s) 10, 217, 365, 416, 534, 547, 569
HpySE526I ACGT 1 cut(s) 482
Hsp92II CATG 7 cut(s) 88, 442, 582, 866, 1025, 1065, 1100
KpnI GGTACC 1 cut(s) 737
KroI GCCGGC 1 cut(s) 918
KroNI GCCGGC 1 cut(s) 920
Kzo9I GATC 5 cut(s) 489, 565, 683, 796, 871
Lsp1109I GCAGC 2 cut(s) 356, 839
LweI GCATC 2 cut(s) 983, 1005
MaeI CTAG 4 cut(s) 59, 279, 645, 902
MaeII ACGT 1 cut(s) 482
MaeIII GTNAC 2 cut(s) 250, 419
MalI GATC 5 cut(s) 491, 567, 685, 798, 873
MboI GATC 5 cut(s) 489, 565, 683, 796, 871
MboII GAAGA 5 cut(s) 13, 25, 157, 200, 678
MfeI CAATTG 1 cut(s) 852
MflI RGATCY 2 cut(s) 565, 683
MluCI AATT 4 cut(s) 466, 852, 858, 1049
MlyI GAGTC 2 cut(s) 799, 1097
MmeI TCCRAC 1 cut(s) 714
MnlI CCTC 9 cut(s) 29, 54, 91, 320, 374, 529, 752, 788, 1096
Mph1103I ATGCAT 1 cut(s) 998
MroNI GCCGGC 1 cut(s) 918
MseI TTAA 2 cut(s) 264, 978
MslI CAYNNNNRTG 1 cut(s) 51
MspA1I CMGCKG 1 cut(s) 369
MspI CCGG 4 cut(s) 99, 506, 512, 919
MspR9I CCNGG 3 cut(s) 162, 591, 930
MunI CAATTG 1 cut(s) 852
MvaI CCWGG 3 cut(s) 162, 591, 930
MvnI CGCG 1 cut(s) 479
MwoI GCNNNNNNNGC 6 cut(s) 99, 636, 645, 770, 774, 827
NaeI GCCGGC 1 cut(s) 920
NdeII GATC 5 cut(s) 489, 565, 683, 796, 871
NgoMIV GCCGGC 1 cut(s) 918
NlaIII CATG 7 cut(s) 88, 442, 582, 866, 1025, 1065, 1100
NlaIV GGNNCC 3 cut(s) 510, 735, 815
NmuCI GTSAC 1 cut(s) 250
NsiI ATGCAT 1 cut(s) 998
NspI RCATGY 1 cut(s) 1065
PciI ACATGT 1 cut(s) 1061
PdiI GCCGGC 1 cut(s) 920
PfeI GAWTC 6 cut(s) 114, 543, 560, 696, 845, 887
PfoI TCCNGGA 1 cut(s) 928
PkrI GCNGC 2 cut(s) 371, 829
PleI GAGTC 2 cut(s) 798, 1097
PpsI GAGTC 2 cut(s) 798, 1097
PpuMI RGGWCCY 1 cut(s) 932
PscI ACATGT 1 cut(s) 1061
PshAI GACNNNNGTC 2 cut(s) 197, 789
Psp5II RGGWCCY 1 cut(s) 932
Psp6I CCWGG 3 cut(s) 160, 589, 928
PspFI CCCAGC 1 cut(s) 1090
PspGI CCWGG 3 cut(s) 160, 589, 928
PspN4I GGNNCC 3 cut(s) 510, 735, 815
PspPI GGNCC 1 cut(s) 932
PspPPI RGGWCCY 1 cut(s) 932
PsrI GAACNNNNNNTAC 2 cut(s) 140, 172
PstI CTGCAG 1 cut(s) 823
PsuI RGATCY 2 cut(s) 565, 683
PvuII CAGCTG 1 cut(s) 369
RsaI GTAC 3 cut(s) 654, 735, 906
RsaNI GTAC 3 cut(s) 653, 734, 905
RseI CAYNNNNRTG 1 cut(s) 51
SalI GTCGAC 1 cut(s) 1100
SaqAI TTAA 2 cut(s) 264, 978
SatI GCNGC 2 cut(s) 370, 828
Sau3AI GATC 5 cut(s) 489, 565, 683, 796, 871
Sau96I GGNCC 1 cut(s) 932
ScaI AGTACT 2 cut(s) 654, 906
SchI GAGTC 2 cut(s) 799, 1097
ScrFI CCNGG 3 cut(s) 162, 591, 930
SfaNI GCATC 2 cut(s) 983, 1005
SfcI CTRYAG 2 cut(s) 675, 819
SinI GGWCC 1 cut(s) 932
SmiMI CAYNNNNRTG 1 cut(s) 51
SmlI CTYRAG 1 cut(s) 779
SmoI CTYRAG 1 cut(s) 779
Sse9I AATT 4 cut(s) 466, 852, 858, 1049
SsiI CCGC 1 cut(s) 477
SspMI CTAG 4 cut(s) 59, 279, 645, 902
StyD4I CCNGG 3 cut(s) 160, 589, 928
TaaI ACNGT 3 cut(s) 152, 199, 988
TaiI ACGT 1 cut(s) 485
TaqI TCGA 8 cut(s) 168, 235, 243, 312, 425, 633, 1075, 1101
TasI AATT 4 cut(s) 466, 852, 858, 1049
TatI WGTACW 2 cut(s) 652, 904
TfiI GAWTC 6 cut(s) 114, 543, 560, 696, 845, 887
Tru1I TTAA 2 cut(s) 264, 978
Tru9I TTAA 2 cut(s) 264, 978
TscAI CASTG 2 cut(s) 808, 1019
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 2 cut(s) 369, 827
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 4 cut(s) 13, 288, 369, 851
TspGWI ACGGA 1 cut(s) 708
TspRI CASTG 2 cut(s) 808, 1019
VpaK11BI GGWCC 1 cut(s) 932
XagI CCTNNNNNAGG 1 cut(s) 606
XapI RAATTY 1 cut(s) 466
XceI RCATGY 1 cut(s) 1065
XmiI GTMKAC 1 cut(s) 1101
XspI CTAG 4 cut(s) 59, 279, 645, 902
ZrmI AGTACT 2 cut(s) 654, 906
Zsp2I ATGCAT 1 cut(s) 998
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.