MD07G1148900.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008440
Physical Location & Seq
Reverse (-)
21736042 .. 21738957
2916 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1148900.v1.1.491

Sequence Viewer

Length: 1170 bp
ATGGACATCTCACACCTCCTCTTTCCCCTTCTCTTCCTCCTCTTCACCCACATCTCTGCACTCAATGATTGCCCGGCTTCCGATTGTGGCGGTGACGACAGCTTCCCAATCCGATTCCCTTTCCGGTTAGTACCCAAACAACCCAAAAACTGCGGTTTCCCGGGCTTCAACCTGGCCTGCAACAGCCACGGTCAAACAACTCTAAAACTTCCAAACTCCGGCGACTTTTTCGTCAGACGAATCAGCTATGCCACACAAGAAATCCAACTTTTCGACCCCGAAAAATGCCTCCCTCGAAGGCTTCTAACCCTCAATCTCTCGGGCTCTCCTTTTGTTACTCCCACGTACCAGAACTACACCTTCCTCAGCTGCCCGGCTTCGTTCACCAAGTCGAGGTTCACTCCCATTGACTGCCTCAGCAACTCCACCACCTCGGTTCTGGCCACCCCTTCCGCCACCCTGGCGAATTCGATGTCCGCCACGTGCCAGATTGTTGACACATTGGAGGTTCCGGTTTGCGGGCAGGTTCAGACGGAGGACGGATTCTCAACCAACCTTGACTGGGATCTTAGTTTGACTTGGTATGAGCCTTCTTGCAGTAGTTGTGAGGTGGAAGGTGGCATTTGTGGATTTATCAGCAACACCACCCAAGAACTCGGTTGCACCTTCAATTCAACAGCAGGAGGATCAAGCTATGGTTTATATGTTTTCAGGATCATTTGCTTGTCCATCACGGTACCAGCCACCGCATGCGCAGTTGTGATTGGCTGCTTTGCTTGTATCAGTGGCAGGGGTAATGCTGCACAGCGAAGGTCCACCGCGGTTAATGCAGAATCGCCACCACAACCCCCCGCCGTTGTAACAGTTGGTTTGGATCAGACAACCATTGAATCCTACCAGATGCTAGTTCTTGGGGAGAGTAAGCGCCTTCCGGGACACAACGATACCACCTGCGCTATATGCTTGTCGGAGTATCTCACCAAAGAGACGGTGAGATGCATACCAGAATGCAAACACTGCTTCCATGCTGATTGTATTGATGAGTGGCTGAAACTGAACAGTTCATGCCCAGTTTGCCGGAACAACCCGTCCCCGGCTCATGTCAACTCAACGTCCCCGGCTCACGTCAACTCAACGTCTCCGGCTCACGTTGACTCAAACAATGTCTGA

Protein Analysis

390

Amino Acids

42.05

Weight (kDa)

5.44

Isoelectric Point (pI)

56.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 24 - 91 5e-17 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 153 - 224 2.2e-07 Wall-associated receptor kinase C-terminal
zf-RING_2 PF13639 317 - 360 9.4e-13 Ring finger domain
zf-rbx1 PF12678 317 - 360 2.3e-06 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 318 - 359 1.4e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 318 - 346 4.3e-07 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0008440)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28040 AT2G25410 AT2G46493 AT2G46494 AT2G46495 AT2G46495 AT5G36001 AT5G36001
fragaria_vesca FvH4_7g16350
malus_domestica MD07G1148900.v1.1
prunus_persica Prupe.2G187400_v2.0.a1
pyrus_communis pycom07g14790
rosa_chinensis RchiOBHm_Chr1g0359361
rosa_laevigata RLG00000027876
rosa_multiflora Rmu_ssc0000358.1_g000002
rosa_roxburghii Rroxscaffold_4G00297050
rosa_rugosa Rorug01G0270500
rosa_samantha Rh1AG283900 Rh1AG284000 Rh1BG250100 Rh1CG268000 Rh1DG279000
rosa_wichuraiana Rw1G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 959
AasI GACNNNNNNGTC 1 cut(s) 230
Acc16I TGCGCA 1 cut(s) 754
Acc36I ACCTGC 2 cut(s) 514, 959
Acc65I GGTACC 1 cut(s) 736
AccB1I GGYRCC 1 cut(s) 736
AccII CGCG 1 cut(s) 821
AciI CCGC 9 cut(s) 90, 153, 453, 477, 519, 747, 819, 821, 852
AclWI GGATC 4 cut(s) 573, 694, 722, 882
AcoI YGGCCR 1 cut(s) 441
AcsI RAATTY 1 cut(s) 466
AcvI CACGTG 1 cut(s) 483
AfaI GTAC 3 cut(s) 132, 347, 738
AfiI CCNNNNNNNGG 5 cut(s) 218, 393, 518, 562, 1093
AgsI TTSAA 4 cut(s) 169, 670, 675, 890
AjiI CACGTC 1 cut(s) 1126
AjnI CCWGG 2 cut(s) 171, 459
AjuI GAANNNNNNNTTGG 2 cut(s) 380, 412
AloI GAACNNNNNNTCC 2 cut(s) 1073, 1105
AluBI AGCT 4 cut(s) 102, 246, 369, 693
AluI AGCT 4 cut(s) 102, 246, 369, 693
Alw26I GTCTC 2 cut(s) 980, 1143
AlwI GGATC 4 cut(s) 573, 694, 722, 882
Ama87I CYCGRG 2 cut(s) 160, 319
AoxI GGCC 2 cut(s) 174, 441
ApeKI GCWGC 3 cut(s) 369, 768, 800
ApoI RAATTY 1 cut(s) 466
ArsI GACNNNNNNTTYG 2 cut(s) 458, 490
Asp718I GGTACC 1 cut(s) 736
AspLEI GCGC 3 cut(s) 755, 927, 956
AspS9I GGNCC 1 cut(s) 813
AsuC2I CCSGG 7 cut(s) 74, 161, 162, 374, 933, 1094, 1118
AsuHPI GGTGA 5 cut(s) 37, 104, 376, 970, 1003
AvaI CYCGRG 2 cut(s) 160, 319
AvaII GGWCC 1 cut(s) 813
BalI TGGCCA 1 cut(s) 443
BanI GGYRCC 1 cut(s) 736
BanII GRGCYC 1 cut(s) 326
BbrPI CACGTG 1 cut(s) 483
BbvCI CCTCAGC 2 cut(s) 365, 416
BbvI GCAGC 3 cut(s) 356, 755, 787
BccI CCATC 1 cut(s) 737
BceAI ACGGC 1 cut(s) 839
BciT130I CCWGG 2 cut(s) 173, 461
BcnI CCSGG 7 cut(s) 74, 161, 162, 374, 933, 1094, 1118
BcoDI GTCTC 2 cut(s) 980, 1143
BfaI CTAG 1 cut(s) 905
BfoI RGCGCY 1 cut(s) 928
BfuAI ACCTGC 2 cut(s) 514, 959
BglI GCCNNNNNGGC 1 cut(s) 461
BisI GCNGC 3 cut(s) 370, 769, 801
BlsI GCNGC 3 cut(s) 371, 770, 802
Bme1390I CCNGG 9 cut(s) 74, 161, 162, 173, 374, 461, 933, 1094, 1118
Bme18I GGWCC 1 cut(s) 813
BmeT110I CYCGRG 2 cut(s) 160, 319
BmgBI CACGTC 1 cut(s) 1126
BmgT120I GGNCC 1 cut(s) 813
BmiI GGNNCC 2 cut(s) 510, 738
BmrFI CCNGG 9 cut(s) 74, 161, 162, 173, 374, 461, 933, 1094, 1118
BmrI ACTGGG 2 cut(s) 571, 1064
BmsI GCATC 2 cut(s) 891, 986
BmuI ACTGGG 2 cut(s) 571, 1064
BplI GAGNNNNNCTC 2 cut(s) 385, 417
Bpu10I CCTNAGC 2 cut(s) 365, 416
BpuMI CCSGG 7 cut(s) 74, 161, 162, 374, 933, 1094, 1118
BsaAI YACGTR 2 cut(s) 345, 483
BsaJI CCNNGG 7 cut(s) 160, 187, 432, 459, 819, 1092, 1116
BsaWI WCCGGW 2 cut(s) 123, 511
Bsc4I CCNNNNNNNGG 5 cut(s) 218, 393, 518, 562, 1093
Bse1I ACTGG 2 cut(s) 566, 1070
BseBI CCWGG 2 cut(s) 173, 461
BseDI CCNNGG 7 cut(s) 160, 187, 432, 459, 819, 1092, 1116
BseLI CCNNNNNNNGG 5 cut(s) 218, 393, 518, 562, 1093
BseMII CTCAG 2 cut(s) 379, 430
BseNI ACTGG 2 cut(s) 566, 1070
BseRI GAGGAG 2 cut(s) 8, 29
BseXI GCAGC 3 cut(s) 356, 755, 787
BsgI GTGCAG 2 cut(s) 42, 786
Bsh1236I CGCG 1 cut(s) 821
BshFI GGCC 2 cut(s) 176, 443
BshNI GGYRCC 1 cut(s) 736
BsiHKCI CYCGRG 2 cut(s) 160, 319
BslFI GGGAC 3 cut(s) 948, 1075, 1099
BslI CCNNNNNNNGG 5 cut(s) 218, 393, 518, 562, 1093
BsmAI GTCTC 2 cut(s) 980, 1143
BsmBI CGTCTC 2 cut(s) 980, 1143
BsmFI GGGAC 3 cut(s) 948, 1075, 1099
BsmI GAATGC 1 cut(s) 1013
BsnI GGCC 2 cut(s) 176, 443
BsoBI CYCGRG 2 cut(s) 160, 319
Bsp1286I GDGCHC 1 cut(s) 326
Bsp143I GATC 4 cut(s) 565, 686, 714, 874
BspACI CCGC 9 cut(s) 90, 153, 453, 477, 519, 747, 819, 821, 852
BspANI GGCC 2 cut(s) 176, 443
BspCNI CTCAG 2 cut(s) 378, 429
BspFNI CGCG 1 cut(s) 821
BspLI GGNNCC 2 cut(s) 510, 738
BspMI ACCTGC 2 cut(s) 514, 959
BspPI GGATC 4 cut(s) 573, 694, 722, 882
BspT107I GGYRCC 1 cut(s) 736
BsrI ACTGG 2 cut(s) 566, 1070
BssECI CCNNGG 7 cut(s) 160, 187, 432, 459, 819, 1092, 1116
BssMI GATC 4 cut(s) 565, 686, 714, 874
Bst2UI CCWGG 2 cut(s) 173, 461
Bst4CI ACNGT 5 cut(s) 191, 736, 865, 991, 1061
Bst6I CTCTTC 2 cut(s) 38, 47
BstAPI GCANNNNNTGC 1 cut(s) 1017
BstBAI YACGTR 2 cut(s) 345, 483
BstC8I GCNNGC 3 cut(s) 178, 521, 751
BstDEI CTNAG 3 cut(s) 365, 416, 569
BstDSI CCRYGG 2 cut(s) 187, 819
BstFNI CGCG 1 cut(s) 821
BstH2I RGCGCY 1 cut(s) 928
BstHHI GCGC 3 cut(s) 755, 927, 956
BstKTI GATC 4 cut(s) 568, 689, 717, 877
BstMAI GTCTC 2 cut(s) 980, 1143
BstMBI GATC 4 cut(s) 565, 686, 714, 874
BstMWI GCNNNNNNNGC 5 cut(s) 461, 827, 960, 1017, 1074
BstNI CCWGG 2 cut(s) 173, 461
BstNSI RCATGY 1 cut(s) 753
BstSCI CCNGG 9 cut(s) 72, 159, 160, 171, 372, 459, 931, 1092, 1116
BstUI CGCG 1 cut(s) 821
BstV1I GCAGC 3 cut(s) 356, 755, 787
BstX2I RGATCY 1 cut(s) 565
BstYI RGATCY 1 cut(s) 565
BsuRI GGCC 2 cut(s) 176, 443
BtgI CCRYGG 2 cut(s) 187, 819
BtrI CACGTC 1 cut(s) 1126
BtsI GCAGTG 1 cut(s) 1015
BtsIMutI CAGTG 2 cut(s) 790, 1015
BveI ACCTGC 2 cut(s) 514, 959
Cac8I GCNNGC 3 cut(s) 178, 521, 751
CfoI GCGC 3 cut(s) 755, 927, 956
Cfr13I GGNCC 1 cut(s) 813
Cfr42I CCGCGG 1 cut(s) 822
Cfr9I CCCGGG 1 cut(s) 160
Csp6I GTAC 3 cut(s) 131, 346, 737
CviAII CATG 4 cut(s) 750, 1025, 1065, 1100
CviQI GTAC 3 cut(s) 131, 346, 737
DdeI CTNAG 3 cut(s) 365, 416, 569
DpnI GATC 4 cut(s) 567, 688, 716, 876
DpnII GATC 4 cut(s) 565, 686, 714, 874
DrdI GACNNNNNNGTC 1 cut(s) 230
DseDI GACNNNNNNGTC 1 cut(s) 230
EaeI YGGCCR 1 cut(s) 441
Eam1104I CTCTTC 2 cut(s) 38, 47
EarI CTCTTC 2 cut(s) 38, 47
EciI GGCGGA 2 cut(s) 442, 466
Eco24I GRGCYC 1 cut(s) 326
Eco47I GGWCC 1 cut(s) 813
Eco72I CACGTG 1 cut(s) 483
Eco88I CYCGRG 2 cut(s) 160, 319
EcoRI GAATTC 1 cut(s) 466
EcoRII CCWGG 2 cut(s) 171, 459
EcoT22I ATGCAT 1 cut(s) 1001
EcoT38I GRGCYC 1 cut(s) 326
Esp3I CGTCTC 2 cut(s) 980, 1143
FaeI CATG 4 cut(s) 753, 1028, 1068, 1103
FaqI GGGAC 3 cut(s) 948, 1075, 1099
FatI CATG 4 cut(s) 749, 1024, 1064, 1099
FauI CCCGC 2 cut(s) 512, 859
Fnu4HI GCNGC 3 cut(s) 370, 769, 801
FriOI GRGCYC 1 cut(s) 326
Fsp4HI GCNGC 3 cut(s) 370, 769, 801
FspBI CTAG 1 cut(s) 905
FspI TGCGCA 1 cut(s) 754
GlaI GCGC 3 cut(s) 754, 926, 955
GluI GCNGC 3 cut(s) 370, 769, 801
HaeII RGCGCY 1 cut(s) 928
HaeIII GGCC 2 cut(s) 176, 443
HhaI GCGC 3 cut(s) 755, 927, 956
Hin1II CATG 4 cut(s) 753, 1028, 1068, 1103
Hin6I GCGC 3 cut(s) 753, 925, 954
HinP1I GCGC 3 cut(s) 753, 925, 954
HincII GTYRAC 4 cut(s) 496, 1105, 1129, 1153
HindII GTYRAC 4 cut(s) 496, 1105, 1129, 1153
HinfI GANTC 6 cut(s) 114, 240, 543, 833, 890, 1154
HphI GGTGA 5 cut(s) 37, 104, 376, 970, 1003
Hpy166II GTNNAC 7 cut(s) 384, 399, 496, 816, 1105, 1129, 1153
Hpy188I TCNGA 7 cut(s) 82, 113, 236, 531, 879, 970, 1169
Hpy188III TCNNGA 1 cut(s) 712
Hpy8I GTNNAC 7 cut(s) 384, 399, 496, 816, 1105, 1129, 1153
HpyAV CCTTC 9 cut(s) 38, 291, 370, 459, 600, 608, 676, 804, 938
HpyCH4III ACNGT 5 cut(s) 191, 736, 865, 991, 1061
HpyCH4IV ACGT 6 cut(s) 344, 482, 1112, 1125, 1136, 1149
HpyCH4V TGCA 8 cut(s) 59, 180, 597, 663, 803, 830, 999, 1011
HpyF10VI GCNNNNNNNGC 5 cut(s) 461, 827, 960, 1017, 1074
HpyF3I CTNAG 3 cut(s) 365, 416, 569
HpySE526I ACGT 6 cut(s) 344, 482, 1112, 1125, 1136, 1149
Hsp92II CATG 4 cut(s) 753, 1028, 1068, 1103
HspAI GCGC 3 cut(s) 753, 925, 954
KpnI GGTACC 1 cut(s) 740
KspI CCGCGG 1 cut(s) 822
Kzo9I GATC 4 cut(s) 565, 686, 714, 874
Lsp1109I GCAGC 3 cut(s) 356, 755, 787
LweI GCATC 2 cut(s) 891, 986
MaeI CTAG 1 cut(s) 905
MaeII ACGT 6 cut(s) 344, 482, 1112, 1125, 1136, 1149
MaeIII GTNAC 3 cut(s) 92, 334, 859
MalI GATC 4 cut(s) 567, 688, 716, 876
MboI GATC 4 cut(s) 565, 686, 714, 874
MboII GAAGA 2 cut(s) 25, 34
MflI RGATCY 1 cut(s) 565
MhlI GDGCHC 1 cut(s) 326
MlsI TGGCCA 1 cut(s) 443
MluCI AATT 2 cut(s) 466, 670
MluNI TGGCCA 1 cut(s) 443
MlyI GAGTC 1 cut(s) 1148
MmeI TCCRAC 2 cut(s) 289, 948
Mox20I TGGCCA 1 cut(s) 443
Mph1103I ATGCAT 1 cut(s) 1001
MscI TGGCCA 1 cut(s) 443
MseI TTAA 1 cut(s) 825
Msp20I TGGCCA 1 cut(s) 443
MspA1I CMGCKG 2 cut(s) 369, 821
MspR9I CCNGG 9 cut(s) 74, 161, 162, 173, 374, 461, 933, 1094, 1118
Mva1269I GAATGC 1 cut(s) 1013
MvaI CCWGG 2 cut(s) 173, 461
MvnI CGCG 1 cut(s) 821
MwoI GCNNNNNNNGC 5 cut(s) 461, 827, 960, 1017, 1074
NciI CCSGG 7 cut(s) 74, 161, 162, 374, 933, 1094, 1118
NdeII GATC 4 cut(s) 565, 686, 714, 874
NlaIII CATG 4 cut(s) 753, 1028, 1068, 1103
NlaIV GGNNCC 2 cut(s) 510, 738
NmuCI GTSAC 1 cut(s) 92
NsbI TGCGCA 1 cut(s) 754
NsiI ATGCAT 1 cut(s) 1001
NspI RCATGY 1 cut(s) 753
PaeI GCATGC 1 cut(s) 753
PaqCI CACCTGC 1 cut(s) 959
PctI GAATGC 1 cut(s) 1013
PfeI GAWTC 5 cut(s) 114, 240, 543, 833, 890
PfoI TCCNGGA 1 cut(s) 931
PkrI GCNGC 3 cut(s) 371, 770, 802
PleI GAGTC 1 cut(s) 1148
PmaCI CACGTG 1 cut(s) 483
PmlI CACGTG 1 cut(s) 483
PpsI GAGTC 1 cut(s) 1148
Ppu21I YACGTR 2 cut(s) 345, 483
Psp6I CCWGG 2 cut(s) 171, 459
PspCI CACGTG 1 cut(s) 483
PspGI CCWGG 2 cut(s) 171, 459
PspN4I GGNNCC 2 cut(s) 510, 738
PspPI GGNCC 1 cut(s) 813
PsuI RGATCY 1 cut(s) 565
PvuII CAGCTG 1 cut(s) 369
RsaI GTAC 3 cut(s) 132, 347, 738
RsaNI GTAC 3 cut(s) 131, 346, 737
SacII CCGCGG 1 cut(s) 822
SaqAI TTAA 1 cut(s) 825
SatI GCNGC 3 cut(s) 370, 769, 801
Sau3AI GATC 4 cut(s) 565, 686, 714, 874
Sau96I GGNCC 1 cut(s) 813
SchI GAGTC 1 cut(s) 1148
ScrFI CCNGG 9 cut(s) 74, 161, 162, 173, 374, 461, 933, 1094, 1118
SduI GDGCHC 1 cut(s) 326
SfaNI GCATC 2 cut(s) 891, 986
Sfr303I CCGCGG 1 cut(s) 822
SgrBI CCGCGG 1 cut(s) 822
SinI GGWCC 1 cut(s) 813
SmaI CCCGGG 1 cut(s) 162
SphI GCATGC 1 cut(s) 753
Sse9I AATT 2 cut(s) 466, 670
SsiI CCGC 9 cut(s) 90, 153, 453, 477, 519, 747, 819, 821, 852
SspMI CTAG 1 cut(s) 905
StyD4I CCNGG 9 cut(s) 72, 159, 160, 171, 372, 459, 931, 1092, 1116
TaaI ACNGT 5 cut(s) 191, 736, 865, 991, 1061
TaiI ACGT 6 cut(s) 347, 485, 1115, 1128, 1139, 1152
TaqI TCGA 4 cut(s) 273, 295, 392, 470
TasI AATT 2 cut(s) 466, 670
TfiI GAWTC 5 cut(s) 114, 240, 543, 833, 890
Tru1I TTAA 1 cut(s) 825
Tru9I TTAA 1 cut(s) 825
TscAI CASTG 2 cut(s) 790, 1022
TseFI GTSAC 1 cut(s) 92
TseI GCWGC 3 cut(s) 369, 768, 800
Tsp45I GTSAC 1 cut(s) 92
TspDTI ATGAA 1 cut(s) 1053
TspGWI ACGGA 2 cut(s) 548, 555
TspMI CCCGGG 1 cut(s) 160
TspRI CASTG 2 cut(s) 790, 1022
VpaK11BI GGWCC 1 cut(s) 813
XapI RAATTY 1 cut(s) 466
XceI RCATGY 1 cut(s) 753
XcmI CCANNNNNNNNNTGG 1 cut(s) 436
XmaI CCCGGG 1 cut(s) 160
XspI CTAG 1 cut(s) 905
Zsp2I ATGCAT 1 cut(s) 1001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.