Rroxscaffold_4G00297050

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0008440
Physical Location & Seq
Forward (+)
17063916 .. 17066101
2186 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00297050.1

Sequence Viewer

Length: 1131 bp
ATGGACATCTCAGATATCCTCTTTCTCTTTCTCTTCCTATTTCCTCATTTCTATGCACTAGAATTAGGCGACTGTCCACCCTCCATGTGTGGCAACACCGGCTTTCCTATCCGGTTCCCTTTCGGGCTACAAGGCTATCAACCCAAAAACTGTTCTTACCCAGGTTTCGACCTAACCTGCAATACACAGGGCATAACAGTCCTGAGACTTCCAAACTCTGGAGAATTTTTTGTCCGAGCAATCGATTATGTCACACAAGAGATTCAACTCTATGACCCTAGTAGTTGCCTTCCCAAAAGGCTTCTAACCCTCAACCTTTCAGGCTCTCCTTTTGTTGCTGCTTTTTATCAGAACTACACCTTCCTCAGCTGCCCTGCTTCATTCACAGAGTCAAAGTTCACCCCCATTGGTTGCCTCAGTAACTCCACAACCTCAGTCTTGGCAACCCCTTCCATCAGCCTCGCAAATTCAATGTCTAAGGTGTGCAAGATCATTGCTACAATGCCAGTTCCGGTTTCTGGGATTGTTGAGTCTGAGGATGGATTCTCAACCAACTTTGATTCAGACCTTAGCTTGACTTGGTATGAGCCTGGTTGTACTCCTTGTGAAGGTGAAGGTGGCATTTGTGGCTTCGAGAGCAACACTAGCCAAGTACTTGGTTGCTTCTACAATTCTAAAGGCAGATCAGGCGACGGTTTCCAAGTTTTCAGGATTATTTGTGTCTCCATCGCGGTACCAGCCCTCACAATGGCGATTGGGATCGCTTGCTTTGCATGCATCAAGGACAGGAGTGGGATCAGTCCTGGTCGCACTGCACAGCGGAACCCCACCGCAGTTGCAGCAGAACCCGAACAACAATCCAGAATAGTAATGAATGGTCTGGATCAGACCACCATTGAATCCTATCAAAAGCTAGTTCTTGGTGAGAGCCGGCGGCTTCCAGGACCCAACGACACCACTTGCACTATATGCTTATCAGAGTATCTCACCAAAGACACGGTAAGATGCATCCCAGAGTGTAAACACTGCTTCCATGCTGAATGTGTTGATGAGTGGCTACGATTGAACGGTACATGTCCAGTTTGTCGAAACTCCCCCTCCCCAGCTCATGTCAACTCAGATGGGGTCTGA

Protein Analysis

376

Amino Acids

40.9

Weight (kDa)

5.02

Isoelectric Point (pI)

55.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 25 - 92 1.2e-17 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 154 - 225 1.4e-08 Wall-associated receptor kinase C-terminal
zf-RING_2 PF13639 320 - 363 5.9e-13 Ring finger domain
zf-rbx1 PF12678 320 - 363 3.1e-06 RING-H2 zinc finger domain
zf-RING_11 PF17123 321 - 349 1.5e-06 RING-like zinc finger
zf-C3HC4_2 PF13923 321 - 362 5.9e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0008440)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28040 AT2G25410 AT2G46493 AT2G46494 AT2G46495 AT2G46495 AT5G36001 AT5G36001
fragaria_vesca FvH4_7g16350
malus_domestica MD07G1148900.v1.1
prunus_persica Prupe.2G187400_v2.0.a1
pyrus_communis pycom07g14790
rosa_chinensis RchiOBHm_Chr1g0359361
rosa_laevigata RLG00000027876
rosa_multiflora Rmu_ssc0000358.1_g000002
rosa_roxburghii Rroxscaffold_4G00297050
rosa_rugosa Rorug01G0270500
rosa_samantha Rh1AG283900 Rh1AG284000 Rh1BG250100 Rh1CG268000 Rh1DG279000
rosa_wichuraiana Rw1G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 185
Acc65I GGTACC 1 cut(s) 733
AccB1I GGYRCC 1 cut(s) 733
AccB7I CCANNNNNTGG 1 cut(s) 218
AccII CGCG 1 cut(s) 731
AciI CCGC 4 cut(s) 731, 820, 831, 934
AclWI GGATC 3 cut(s) 767, 803, 891
AcsI RAATTY 2 cut(s) 224, 466
AfaI GTAC 4 cut(s) 598, 654, 735, 1072
AfiI CCNNNNNNNGG 4 cut(s) 218, 518, 608, 748
AflIII ACRYGT 1 cut(s) 1073
AgsI TTSAA 4 cut(s) 266, 471, 899, 1066
AjnI CCWGG 4 cut(s) 160, 589, 802, 940
AluBI AGCT 4 cut(s) 369, 573, 913, 1106
AluI AGCT 4 cut(s) 369, 573, 913, 1106
Alw26I GTCTC 2 cut(s) 199, 727
AlwI GGATC 3 cut(s) 767, 803, 891
ApeKI GCWGC 3 cut(s) 338, 369, 839
ApoI RAATTY 2 cut(s) 224, 466
ArsI GACNNNNNNTTYG 2 cut(s) 458, 490
Asp718I GGTACC 1 cut(s) 733
AspS9I GGNCC 1 cut(s) 944
AsuHPI GGTGA 4 cut(s) 391, 623, 935, 979
AvaII GGWCC 1 cut(s) 944
BanI GGYRCC 1 cut(s) 733
BbvCI CCTCAGC 1 cut(s) 365
BbvI GCAGC 3 cut(s) 325, 356, 851
BccI CCATC 4 cut(s) 461, 533, 734, 1115
BciT130I CCWGG 4 cut(s) 162, 591, 804, 942
BcoDI GTCTC 2 cut(s) 199, 727
BfaI CTAG 4 cut(s) 59, 279, 645, 914
BfuAI ACCTGC 1 cut(s) 185
BisI GCNGC 4 cut(s) 339, 370, 840, 935
BlsI GCNGC 4 cut(s) 340, 371, 841, 936
BmcAI AGTACT 1 cut(s) 654
Bme1390I CCNGG 4 cut(s) 162, 591, 804, 942
Bme18I GGWCC 1 cut(s) 944
BmgT120I GGNCC 1 cut(s) 944
BmiI GGNNCC 4 cut(s) 116, 735, 824, 946
BmrFI CCNGG 4 cut(s) 162, 591, 804, 942
BmsI GCATC 3 cut(s) 786, 995, 1017
BpmI CTGGAG 1 cut(s) 240
Bpu10I CCTNAGC 2 cut(s) 365, 569
Bsa29I ATCGAT 1 cut(s) 243
BsaBI GATNNNNATC 1 cut(s) 758
BsaJI CCNNGG 1 cut(s) 160
BsaWI WCCGGW 2 cut(s) 111, 511
BsaXI ACNNNNNCTCC 2 cut(s) 1082, 1112
Bsc4I CCNNNNNNNGG 4 cut(s) 218, 518, 608, 748
Bse118I RCCGGY 2 cut(s) 98, 930
Bse1I ACTGG 2 cut(s) 506, 1079
Bse3DI GCAATG 1 cut(s) 492
Bse8I GATNNNNATC 1 cut(s) 758
BseBI CCWGG 4 cut(s) 162, 591, 804, 942
BseCI ATCGAT 1 cut(s) 243
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 2 cut(s) 544, 1008
BseJI GATNNNNATC 1 cut(s) 758
BseLI CCNNNNNNNGG 4 cut(s) 218, 518, 608, 748
BseMI GCAATG 1 cut(s) 492
BseMII CTCAG 7 cut(s) 24, 194, 379, 430, 447, 525, 1131
BseNI ACTGG 2 cut(s) 506, 1079
BseXI GCAGC 3 cut(s) 325, 356, 851
BseYI CCCAGC 1 cut(s) 1102
BsgI GTGCAG 1 cut(s) 798
Bsh1236I CGCG 1 cut(s) 731
BshNI GGYRCC 1 cut(s) 733
BshVI ATCGAT 1 cut(s) 243
BsiSI CCGG 4 cut(s) 99, 112, 512, 931
BslI CCNNNNNNNGG 4 cut(s) 218, 518, 608, 748
BsmAI GTCTC 2 cut(s) 199, 727
Bsp143I GATC 5 cut(s) 489, 683, 759, 795, 883
BspACI CCGC 4 cut(s) 731, 820, 831, 934
BspCNI CTCAG 7 cut(s) 23, 195, 378, 429, 446, 526, 1130
BspDI ATCGAT 1 cut(s) 243
BspFNI CGCG 1 cut(s) 731
BspLI GGNNCC 4 cut(s) 116, 735, 824, 946
BspMI ACCTGC 1 cut(s) 185
BspPI GGATC 3 cut(s) 767, 803, 891
BspT107I GGYRCC 1 cut(s) 733
BsrDI GCAATG 1 cut(s) 492
BsrFI RCCGGY 2 cut(s) 98, 930
BsrI ACTGG 2 cut(s) 506, 1079
BssAI RCCGGY 2 cut(s) 98, 930
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 5 cut(s) 489, 683, 759, 795, 883
Bst2UI CCWGG 4 cut(s) 162, 591, 804, 942
Bst4CI ACNGT 6 cut(s) 74, 152, 199, 695, 1000, 1070
Bst6I CTCTTC 1 cut(s) 38
BstAPI GCANNNNNTGC 1 cut(s) 969
BstC8I GCNNGC 3 cut(s) 766, 775, 932
BstDEI CTNAG 9 cut(s) 10, 203, 365, 416, 433, 477, 534, 569, 1117
BstENI CCTNNNNNAGG 1 cut(s) 606
BstF5I GGATG 2 cut(s) 544, 1008
BstFNI CGCG 1 cut(s) 731
BstKTI GATC 5 cut(s) 492, 686, 762, 798, 886
BstMAI GTCTC 2 cut(s) 199, 727
BstMBI GATC 5 cut(s) 489, 683, 759, 795, 883
BstNI CCWGG 4 cut(s) 162, 591, 804, 942
BstNSI RCATGY 2 cut(s) 777, 1077
BstSCI CCNGG 4 cut(s) 160, 589, 802, 940
BstUI CGCG 1 cut(s) 731
BstV1I GCAGC 3 cut(s) 325, 356, 851
BstXI CCANNNNNNTGG 1 cut(s) 656
Bsu15I ATCGAT 1 cut(s) 243
BsuTUI ATCGAT 1 cut(s) 243
BtgZI GCGATG 1 cut(s) 712
BtsCI GGATG 2 cut(s) 544, 1008
BtsI GCAGTG 2 cut(s) 810, 1024
BtsIMutI CAGTG 2 cut(s) 810, 1024
BveI ACCTGC 1 cut(s) 185
Cac8I GCNNGC 3 cut(s) 766, 775, 932
Cfr10I RCCGGY 2 cut(s) 98, 930
Cfr13I GGNCC 1 cut(s) 944
ClaI ATCGAT 1 cut(s) 243
Csp6I GTAC 4 cut(s) 597, 653, 734, 1071
CspCI CAANNNNNGTGG 2 cut(s) 817, 852
CviAII CATG 5 cut(s) 85, 774, 1034, 1074, 1109
CviQI GTAC 4 cut(s) 597, 653, 734, 1071
DdeI CTNAG 9 cut(s) 10, 203, 365, 416, 433, 477, 534, 569, 1117
DpnI GATC 5 cut(s) 491, 685, 761, 797, 885
DpnII GATC 5 cut(s) 489, 683, 759, 795, 883
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco32I GATATC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 944
EcoNI CCTNNNNNAGG 1 cut(s) 606
EcoO109I RGGNCCY 1 cut(s) 944
EcoRII CCWGG 4 cut(s) 160, 589, 802, 940
EcoRV GATATC 1 cut(s) 16
EcoT22I ATGCAT 2 cut(s) 779, 1010
FaeI CATG 5 cut(s) 88, 777, 1037, 1077, 1112
FatI CATG 5 cut(s) 84, 773, 1033, 1073, 1108
Fnu4HI GCNGC 4 cut(s) 339, 370, 840, 935
FokI GGATG 2 cut(s) 551, 995
Fsp4HI GCNGC 4 cut(s) 339, 370, 840, 935
FspBI CTAG 4 cut(s) 59, 279, 645, 914
GluI GCNGC 4 cut(s) 339, 370, 840, 935
GsaI CCCAGC 1 cut(s) 1106
GsuI CTGGAG 1 cut(s) 240
HapII CCGG 4 cut(s) 99, 112, 512, 931
Hin1II CATG 5 cut(s) 88, 777, 1037, 1077, 1112
HincII GTYRAC 1 cut(s) 1114
HindII GTYRAC 1 cut(s) 1114
HinfI GANTC 6 cut(s) 262, 389, 530, 543, 560, 899
HpaII CCGG 4 cut(s) 99, 112, 512, 931
HphI GGTGA 4 cut(s) 391, 623, 935, 979
Hpy166II GTNNAC 4 cut(s) 77, 399, 1022, 1114
Hpy188I TCNGA 9 cut(s) 13, 236, 351, 535, 565, 888, 979, 1120, 1130
Hpy188III TCNNGA 6 cut(s) 202, 219, 634, 709, 861, 881
Hpy8I GTNNAC 4 cut(s) 77, 399, 1022, 1114
Hpy99I CGWCG 1 cut(s) 695
HpyAV CCTTC 5 cut(s) 299, 370, 459, 602, 608
HpyCH4III ACNGT 6 cut(s) 74, 152, 199, 695, 1000, 1070
HpyCH4V TGCA 9 cut(s) 56, 180, 486, 773, 777, 815, 839, 963, 1008
HpyF3I CTNAG 9 cut(s) 10, 203, 365, 416, 433, 477, 534, 569, 1117
Hsp92II CATG 5 cut(s) 88, 777, 1037, 1077, 1112
KpnI GGTACC 1 cut(s) 737
KroI GCCGGC 1 cut(s) 930
KroNI GCCGGC 1 cut(s) 932
Kzo9I GATC 5 cut(s) 489, 683, 759, 795, 883
Lsp1109I GCAGC 3 cut(s) 325, 356, 851
LweI GCATC 3 cut(s) 786, 995, 1017
MaeI CTAG 4 cut(s) 59, 279, 645, 914
MaeIII GTNAC 2 cut(s) 250, 419
MalI GATC 5 cut(s) 491, 685, 761, 797, 885
MboI GATC 5 cut(s) 489, 683, 759, 795, 883
MboII GAAGA 1 cut(s) 25
MluCI AATT 4 cut(s) 62, 224, 466, 670
MlyI GAGTC 2 cut(s) 398, 539
Mph1103I ATGCAT 2 cut(s) 779, 1010
MroNI GCCGGC 1 cut(s) 930
MslI CAYNNNNRTG 1 cut(s) 51
MspA1I CMGCKG 2 cut(s) 369, 820
MspI CCGG 4 cut(s) 99, 112, 512, 931
MspR9I CCNGG 4 cut(s) 162, 591, 804, 942
MvaI CCWGG 4 cut(s) 162, 591, 804, 942
MvnI CGCG 1 cut(s) 731
NaeI GCCGGC 1 cut(s) 932
NdeII GATC 5 cut(s) 489, 683, 759, 795, 883
NgoMIV GCCGGC 1 cut(s) 930
NlaIII CATG 5 cut(s) 88, 777, 1037, 1077, 1112
NlaIV GGNNCC 4 cut(s) 116, 735, 824, 946
NmuCI GTSAC 1 cut(s) 250
NsiI ATGCAT 2 cut(s) 779, 1010
NspI RCATGY 2 cut(s) 777, 1077
PaeI GCATGC 1 cut(s) 777
PciI ACATGT 1 cut(s) 1073
PdiI GCCGGC 1 cut(s) 932
PfeI GAWTC 4 cut(s) 262, 543, 560, 899
PflMI CCANNNNNTGG 1 cut(s) 218
PfoI TCCNGGA 1 cut(s) 940
PkrI GCNGC 4 cut(s) 340, 371, 841, 936
PleI GAGTC 2 cut(s) 397, 538
PpsI GAGTC 2 cut(s) 397, 538
PpuMI RGGWCCY 1 cut(s) 944
PscI ACATGT 1 cut(s) 1073
Psp5II RGGWCCY 1 cut(s) 944
Psp6I CCWGG 4 cut(s) 160, 589, 802, 940
PspFI CCCAGC 1 cut(s) 1102
PspGI CCWGG 4 cut(s) 160, 589, 802, 940
PspN4I GGNNCC 4 cut(s) 116, 735, 824, 946
PspPI GGNCC 1 cut(s) 944
PspPPI RGGWCCY 1 cut(s) 944
PvuII CAGCTG 1 cut(s) 369
RsaI GTAC 4 cut(s) 598, 654, 735, 1072
RsaNI GTAC 4 cut(s) 597, 653, 734, 1071
RseI CAYNNNNRTG 1 cut(s) 51
SatI GCNGC 4 cut(s) 339, 370, 840, 935
Sau3AI GATC 5 cut(s) 489, 683, 759, 795, 883
Sau96I GGNCC 1 cut(s) 944
ScaI AGTACT 1 cut(s) 654
SchI GAGTC 2 cut(s) 398, 539
ScrFI CCNGG 4 cut(s) 162, 591, 804, 942
SfaNI GCATC 3 cut(s) 786, 995, 1017
SinI GGWCC 1 cut(s) 944
SmiMI CAYNNNNRTG 1 cut(s) 51
SphI GCATGC 1 cut(s) 777
Sse9I AATT 4 cut(s) 62, 224, 466, 670
SsiI CCGC 4 cut(s) 731, 820, 831, 934
SspMI CTAG 4 cut(s) 59, 279, 645, 914
StyD4I CCNGG 4 cut(s) 160, 589, 802, 940
TaaI ACNGT 6 cut(s) 74, 152, 199, 695, 1000, 1070
TaqI TCGA 4 cut(s) 168, 243, 633, 1087
TasI AATT 4 cut(s) 62, 224, 466, 670
TatI WGTACW 2 cut(s) 596, 652
TauI GCSGC 1 cut(s) 937
TfiI GAWTC 4 cut(s) 262, 543, 560, 899
TscAI CASTG 2 cut(s) 817, 1031
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 3 cut(s) 338, 369, 839
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 2 cut(s) 369, 887
TspRI CASTG 2 cut(s) 817, 1031
Van91I CCANNNNNTGG 1 cut(s) 218
VpaK11BI GGWCC 1 cut(s) 944
XagI CCTNNNNNAGG 1 cut(s) 606
XapI RAATTY 2 cut(s) 224, 466
XceI RCATGY 2 cut(s) 777, 1077
XspI CTAG 4 cut(s) 59, 279, 645, 914
ZrmI AGTACT 1 cut(s) 654
Zsp2I ATGCAT 2 cut(s) 779, 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.