Rh1BG250100

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0008440
Physical Location & Seq
Reverse (-)
37894281 .. 37896261
1981 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG250100.1

Sequence Viewer

Length: 1047 bp
ATGTGTGGCAACACCGACTTTCCTATCCGGTTCCCTTTCGGGCTACAAGGCTATCAACCCAAAAACTGTTCTTACCCAGGTTTCGACCTAACCTGCAATACACAGGGCATAACAGTCCTGAGACTTCCAAACTCTGGAGAATTTTTTGTCCGAGCAATCGATTATGTCACACAAGAGATTCAACTCTATGACCCTACTAGTTGCCTTCCCAAAAGGCTTCTAACCCTCAACCTTTCAGGCTCTCCTTTTGTTGCTGCTTTTTATCAGAACTACACCTTCCTCAGCTGCCCTGCTTCATTCACAGAGTCAAAGTTCACCCCCATTGGTTGCCTTAGTAACTCCACAACCTCAGTCTTGGCAACCCCTTCCAACAGACTCGCAAATTCAATGTCTGAGGTGTGCAAGATCATCGCTACAATGCCAGTTCCGGTTTCTGGGATAGTTGAGTCTGAGGATGGATTCTCAACCAACTTTGATTCAGACCTTGGTTTGACGTGGTATGAGCCTGGTTGTACTCCTTGTGAAGGTGAAGGTGGCATTTGTGGCTTTGAGAGCAACACTAGCCAAGTACTTGGTTGCTTCTACAATTCTAAAGGCAGATCAGGCGACGGTTTCCAAGTTTTCAGGATTATTTGTGTCTCCATCGCGGTACCAGCCCTCACAATGGCGATTGGGATCGCTTGCTTTGCATGCATCAAGGACAGGAGTGGGATCAGTCCTGGTCGCACTGCACAGCGGAACCCTACTGCAGTTGCAGCAGAACCCGAACAACAATCCACAATAGTAATTAATGGTCTGGATCAGACCACCATTGAATCCTATCAAAAGCTAGTTCTTGGTGAGAGCCGGCGGCTTCCAGGACCCAACGACACCACTTGCACTATATGCTTATCAGAGTATCTCGCCAAAGACACGGTAAGATGCATCCCAGAGTGTAAACACTGCTTCCATGCTGAATGTGTTGATGAGTGGCTGCGATTGAACAGTACATGTCCAGTTTGTCGAAACTCCCCCTCCCCAGCTCATGTCAACTCAGATGGGGTCTGA

Protein Analysis

348

Amino Acids

37.7

Weight (kDa)

5.01

Isoelectric Point (pI)

54.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 2 - 64 2.6e-16 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 121 - 197 1.1e-08 Wall-associated receptor kinase C-terminal
zf-RING_2 PF13639 292 - 335 4.4e-13 Ring finger domain
zf-rbx1 PF12678 292 - 335 2.8e-06 RING-H2 zinc finger domain
zf-RING_11 PF17123 293 - 321 1.4e-06 RING-like zinc finger
zf-C3HC4_2 PF13923 293 - 334 5e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0008440)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28040 AT2G25410 AT2G46493 AT2G46494 AT2G46495 AT2G46495 AT5G36001 AT5G36001
fragaria_vesca FvH4_7g16350
malus_domestica MD07G1148900.v1.1
prunus_persica Prupe.2G187400_v2.0.a1
pyrus_communis pycom07g14790
rosa_chinensis RchiOBHm_Chr1g0359361
rosa_laevigata RLG00000027876
rosa_multiflora Rmu_ssc0000358.1_g000002
rosa_roxburghii Rroxscaffold_4G00297050
rosa_rugosa Rorug01G0270500
rosa_samantha Rh1AG283900 Rh1AG284000 Rh1BG250100 Rh1CG268000 Rh1DG279000
rosa_wichuraiana Rw1G025210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 101
Acc65I GGTACC 1 cut(s) 649
AccB1I GGYRCC 1 cut(s) 649
AccB7I CCANNNNNTGG 1 cut(s) 134
AccII CGCG 1 cut(s) 647
AciI CCGC 3 cut(s) 647, 736, 850
AclWI GGATC 3 cut(s) 683, 719, 807
AcsI RAATTY 2 cut(s) 140, 382
AfaI GTAC 4 cut(s) 514, 570, 651, 988
AfiI CCNNNNNNNGG 4 cut(s) 134, 434, 524, 664
AflIII ACRYGT 1 cut(s) 989
AgsI TTSAA 4 cut(s) 182, 387, 815, 982
AhlI ACTAGT 1 cut(s) 197
AjiI CACGTC 1 cut(s) 495
AjnI CCWGG 4 cut(s) 76, 505, 718, 856
AluBI AGCT 3 cut(s) 285, 829, 1022
AluI AGCT 3 cut(s) 285, 829, 1022
Alw26I GTCTC 2 cut(s) 115, 643
AlwI GGATC 3 cut(s) 683, 719, 807
ApeKI GCWGC 4 cut(s) 254, 285, 755, 973
ApoI RAATTY 2 cut(s) 140, 382
ArsI GACNNNNNNTTYG 2 cut(s) 374, 406
AseI ATTAAT 1 cut(s) 789
Asp718I GGTACC 1 cut(s) 649
AspS9I GGNCC 1 cut(s) 860
AsuHPI GGTGA 3 cut(s) 307, 539, 851
AvaII GGWCC 1 cut(s) 860
BanI GGYRCC 1 cut(s) 649
BbvCI CCTCAGC 1 cut(s) 281
BbvI GCAGC 4 cut(s) 241, 272, 767, 960
BccI CCATC 3 cut(s) 449, 650, 1031
BcgI CGANNNNNNTGC 2 cut(s) 391, 425
BciT130I CCWGG 4 cut(s) 78, 507, 720, 858
BcoDI GTCTC 2 cut(s) 115, 643
BcuI ACTAGT 1 cut(s) 197
BfaI CTAG 3 cut(s) 198, 561, 830
BfmI CTRYAG 1 cut(s) 747
BfuAI ACCTGC 1 cut(s) 101
BisI GCNGC 5 cut(s) 255, 286, 756, 851, 974
BlsI GCNGC 5 cut(s) 256, 287, 757, 852, 975
BmcAI AGTACT 1 cut(s) 570
Bme1390I CCNGG 4 cut(s) 78, 507, 720, 858
Bme18I GGWCC 1 cut(s) 860
BmgBI CACGTC 1 cut(s) 495
BmgT120I GGNCC 1 cut(s) 860
BmiI GGNNCC 4 cut(s) 32, 651, 740, 862
BmrFI CCNGG 4 cut(s) 78, 507, 720, 858
BmsI GCATC 3 cut(s) 702, 911, 933
BpmI CTGGAG 1 cut(s) 156
Bpu10I CCTNAGC 1 cut(s) 281
Bsa29I ATCGAT 1 cut(s) 159
BsaBI GATNNNNATC 1 cut(s) 674
BsaJI CCNNGG 2 cut(s) 76, 484
BsaWI WCCGGW 2 cut(s) 27, 427
BsaXI ACNNNNNCTCC 2 cut(s) 998, 1028
Bsc4I CCNNNNNNNGG 4 cut(s) 134, 434, 524, 664
Bse118I RCCGGY 1 cut(s) 846
Bse1I ACTGG 2 cut(s) 422, 995
Bse8I GATNNNNATC 1 cut(s) 674
BseBI CCWGG 4 cut(s) 78, 507, 720, 858
BseCI ATCGAT 1 cut(s) 159
BseDI CCNNGG 2 cut(s) 76, 484
BseGI GGATG 2 cut(s) 460, 924
BseJI GATNNNNATC 1 cut(s) 674
BseLI CCNNNNNNNGG 4 cut(s) 134, 434, 524, 664
BseMII CTCAG 6 cut(s) 110, 295, 363, 384, 441, 1047
BseNI ACTGG 2 cut(s) 422, 995
BseXI GCAGC 4 cut(s) 241, 272, 767, 960
BseYI CCCAGC 1 cut(s) 1018
BsgI GTGCAG 1 cut(s) 714
Bsh1236I CGCG 1 cut(s) 647
BshNI GGYRCC 1 cut(s) 649
BshVI ATCGAT 1 cut(s) 159
BsiSI CCGG 3 cut(s) 28, 428, 847
BslI CCNNNNNNNGG 4 cut(s) 134, 434, 524, 664
BsmAI GTCTC 2 cut(s) 115, 643
Bsp143I GATC 5 cut(s) 405, 599, 675, 711, 799
BspACI CCGC 3 cut(s) 647, 736, 850
BspCNI CTCAG 6 cut(s) 111, 294, 362, 385, 442, 1046
BspDI ATCGAT 1 cut(s) 159
BspFNI CGCG 1 cut(s) 647
BspLI GGNNCC 4 cut(s) 32, 651, 740, 862
BspMAI CTGCAG 1 cut(s) 751
BspMI ACCTGC 1 cut(s) 101
BspPI GGATC 3 cut(s) 683, 719, 807
BspT107I GGYRCC 1 cut(s) 649
BsrFI RCCGGY 1 cut(s) 846
BsrI ACTGG 2 cut(s) 422, 995
BssAI RCCGGY 1 cut(s) 846
BssECI CCNNGG 2 cut(s) 76, 484
BssMI GATC 5 cut(s) 405, 599, 675, 711, 799
BssT1I CCWWGG 1 cut(s) 484
Bst2UI CCWGG 4 cut(s) 78, 507, 720, 858
Bst4CI ACNGT 5 cut(s) 68, 115, 611, 916, 986
BstAPI GCANNNNNTGC 1 cut(s) 885
BstC8I GCNNGC 3 cut(s) 682, 691, 848
BstDEI CTNAG 7 cut(s) 119, 281, 332, 349, 393, 450, 1033
BstENI CCTNNNNNAGG 1 cut(s) 522
BstF5I GGATG 2 cut(s) 460, 924
BstFNI CGCG 1 cut(s) 647
BstKTI GATC 5 cut(s) 408, 602, 678, 714, 802
BstMAI GTCTC 2 cut(s) 115, 643
BstMBI GATC 5 cut(s) 405, 599, 675, 711, 799
BstMWI GCNNNNNNNGC 9 cut(s) 543, 552, 561, 603, 653, 686, 690, 755, 885
BstNI CCWGG 4 cut(s) 78, 507, 720, 858
BstNSI RCATGY 2 cut(s) 693, 993
BstSCI CCNGG 4 cut(s) 76, 505, 718, 856
BstSFI CTRYAG 1 cut(s) 747
BstUI CGCG 1 cut(s) 647
BstV1I GCAGC 4 cut(s) 241, 272, 767, 960
BstXI CCANNNNNNTGG 1 cut(s) 572
Bsu15I ATCGAT 1 cut(s) 159
BsuTUI ATCGAT 1 cut(s) 159
BtgZI GCGATG 2 cut(s) 394, 628
BtrI CACGTC 1 cut(s) 495
BtsCI GGATG 2 cut(s) 460, 924
BtsI GCAGTG 2 cut(s) 726, 940
BtsIMutI CAGTG 2 cut(s) 726, 940
BveI ACCTGC 1 cut(s) 101
Cac8I GCNNGC 3 cut(s) 682, 691, 848
Cfr10I RCCGGY 1 cut(s) 846
Cfr13I GGNCC 1 cut(s) 860
ClaI ATCGAT 1 cut(s) 159
Csp6I GTAC 4 cut(s) 513, 569, 650, 987
CviAII CATG 4 cut(s) 690, 950, 990, 1025
CviQI GTAC 4 cut(s) 513, 569, 650, 987
DdeI CTNAG 7 cut(s) 119, 281, 332, 349, 393, 450, 1033
DpnI GATC 5 cut(s) 407, 601, 677, 713, 801
DpnII GATC 5 cut(s) 405, 599, 675, 711, 799
Eco130I CCWWGG 1 cut(s) 484
Eco47I GGWCC 1 cut(s) 860
EcoNI CCTNNNNNAGG 1 cut(s) 522
EcoO109I RGGNCCY 1 cut(s) 860
EcoRII CCWGG 4 cut(s) 76, 505, 718, 856
EcoT14I CCWWGG 1 cut(s) 484
EcoT22I ATGCAT 2 cut(s) 695, 926
ErhI CCWWGG 1 cut(s) 484
FaeI CATG 4 cut(s) 693, 953, 993, 1028
FatI CATG 4 cut(s) 689, 949, 989, 1024
Fnu4HI GCNGC 5 cut(s) 255, 286, 756, 851, 974
FokI GGATG 2 cut(s) 467, 911
Fsp4HI GCNGC 5 cut(s) 255, 286, 756, 851, 974
FspBI CTAG 3 cut(s) 198, 561, 830
GluI GCNGC 5 cut(s) 255, 286, 756, 851, 974
GsaI CCCAGC 1 cut(s) 1022
GsuI CTGGAG 1 cut(s) 156
HapII CCGG 3 cut(s) 28, 428, 847
Hin1II CATG 4 cut(s) 693, 953, 993, 1028
HincII GTYRAC 1 cut(s) 1030
HindII GTYRAC 1 cut(s) 1030
HinfI GANTC 7 cut(s) 178, 305, 375, 446, 459, 476, 815
HpaII CCGG 3 cut(s) 28, 428, 847
HphI GGTGA 3 cut(s) 307, 539, 851
Hpy166II GTNNAC 3 cut(s) 315, 938, 1030
Hpy188I TCNGA 9 cut(s) 152, 267, 394, 451, 481, 804, 895, 1036, 1046
Hpy188III TCNNGA 4 cut(s) 118, 135, 625, 797
Hpy8I GTNNAC 3 cut(s) 315, 938, 1030
Hpy99I CGWCG 1 cut(s) 611
HpyAV CCTTC 5 cut(s) 215, 286, 375, 518, 524
HpyCH4III ACNGT 5 cut(s) 68, 115, 611, 916, 986
HpyCH4IV ACGT 1 cut(s) 494
HpyCH4V TGCA 9 cut(s) 96, 402, 689, 693, 731, 749, 755, 879, 924
HpyF10VI GCNNNNNNNGC 9 cut(s) 543, 552, 561, 603, 653, 686, 690, 755, 885
HpyF3I CTNAG 7 cut(s) 119, 281, 332, 349, 393, 450, 1033
HpySE526I ACGT 1 cut(s) 494
Hsp92II CATG 4 cut(s) 693, 953, 993, 1028
KpnI GGTACC 1 cut(s) 653
KroI GCCGGC 1 cut(s) 846
KroNI GCCGGC 1 cut(s) 848
Kzo9I GATC 5 cut(s) 405, 599, 675, 711, 799
Lsp1109I GCAGC 4 cut(s) 241, 272, 767, 960
LweI GCATC 3 cut(s) 702, 911, 933
MaeI CTAG 3 cut(s) 198, 561, 830
MaeII ACGT 1 cut(s) 494
MaeIII GTNAC 2 cut(s) 166, 335
MalI GATC 5 cut(s) 407, 601, 677, 713, 801
MboI GATC 5 cut(s) 405, 599, 675, 711, 799
MluCI AATT 4 cut(s) 140, 382, 586, 786
MlyI GAGTC 3 cut(s) 314, 369, 455
MmeI TCCRAC 1 cut(s) 393
MnlI CCTC 7 cut(s) 236, 290, 358, 388, 445, 668, 1024
Mph1103I ATGCAT 2 cut(s) 695, 926
MroNI GCCGGC 1 cut(s) 846
MseI TTAA 1 cut(s) 789
MspA1I CMGCKG 2 cut(s) 285, 736
MspI CCGG 3 cut(s) 28, 428, 847
MspR9I CCNGG 4 cut(s) 78, 507, 720, 858
MvaI CCWGG 4 cut(s) 78, 507, 720, 858
MvnI CGCG 1 cut(s) 647
MwoI GCNNNNNNNGC 9 cut(s) 543, 552, 561, 603, 653, 686, 690, 755, 885
NaeI GCCGGC 1 cut(s) 848
NdeII GATC 5 cut(s) 405, 599, 675, 711, 799
NgoMIV GCCGGC 1 cut(s) 846
NlaIII CATG 4 cut(s) 693, 953, 993, 1028
NlaIV GGNNCC 4 cut(s) 32, 651, 740, 862
NmuCI GTSAC 1 cut(s) 166
NsiI ATGCAT 2 cut(s) 695, 926
NspI RCATGY 2 cut(s) 693, 993
PaeI GCATGC 1 cut(s) 693
PciI ACATGT 1 cut(s) 989
PdiI GCCGGC 1 cut(s) 848
PfeI GAWTC 4 cut(s) 178, 459, 476, 815
PflMI CCANNNNNTGG 1 cut(s) 134
PfoI TCCNGGA 1 cut(s) 856
PkrI GCNGC 5 cut(s) 256, 287, 757, 852, 975
PleI GAGTC 3 cut(s) 313, 369, 454
PpsI GAGTC 3 cut(s) 313, 369, 454
PpuMI RGGWCCY 1 cut(s) 860
PscI ACATGT 1 cut(s) 989
PshBI ATTAAT 1 cut(s) 789
Psp5II RGGWCCY 1 cut(s) 860
Psp6I CCWGG 4 cut(s) 76, 505, 718, 856
PspFI CCCAGC 1 cut(s) 1018
PspGI CCWGG 4 cut(s) 76, 505, 718, 856
PspN4I GGNNCC 4 cut(s) 32, 651, 740, 862
PspPI GGNCC 1 cut(s) 860
PspPPI RGGWCCY 1 cut(s) 860
PstI CTGCAG 1 cut(s) 751
PvuII CAGCTG 1 cut(s) 285
RsaI GTAC 4 cut(s) 514, 570, 651, 988
RsaNI GTAC 4 cut(s) 513, 569, 650, 987
SaqAI TTAA 1 cut(s) 789
SatI GCNGC 5 cut(s) 255, 286, 756, 851, 974
Sau3AI GATC 5 cut(s) 405, 599, 675, 711, 799
Sau96I GGNCC 1 cut(s) 860
ScaI AGTACT 1 cut(s) 570
SchI GAGTC 3 cut(s) 314, 369, 455
ScrFI CCNGG 4 cut(s) 78, 507, 720, 858
SfaNI GCATC 3 cut(s) 702, 911, 933
SfcI CTRYAG 1 cut(s) 747
SinI GGWCC 1 cut(s) 860
SpeI ACTAGT 1 cut(s) 197
SphI GCATGC 1 cut(s) 693
Sse9I AATT 4 cut(s) 140, 382, 586, 786
SsiI CCGC 3 cut(s) 647, 736, 850
SspMI CTAG 3 cut(s) 198, 561, 830
StyD4I CCNGG 4 cut(s) 76, 505, 718, 856
StyI CCWWGG 1 cut(s) 484
TaaI ACNGT 5 cut(s) 68, 115, 611, 916, 986
TaiI ACGT 1 cut(s) 497
TaqI TCGA 3 cut(s) 84, 159, 1003
TasI AATT 4 cut(s) 140, 382, 586, 786
TatI WGTACW 3 cut(s) 512, 568, 986
TauI GCSGC 1 cut(s) 853
TfiI GAWTC 4 cut(s) 178, 459, 476, 815
Tru1I TTAA 1 cut(s) 789
Tru9I TTAA 1 cut(s) 789
TscAI CASTG 2 cut(s) 733, 947
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 4 cut(s) 254, 285, 755, 973
Tsp45I GTSAC 1 cut(s) 166
TspDTI ATGAA 1 cut(s) 285
TspRI CASTG 2 cut(s) 733, 947
Van91I CCANNNNNTGG 1 cut(s) 134
VpaK11BI GGWCC 1 cut(s) 860
VspI ATTAAT 1 cut(s) 789
XagI CCTNNNNNAGG 1 cut(s) 522
XapI RAATTY 2 cut(s) 140, 382
XceI RCATGY 2 cut(s) 693, 993
XspI CTAG 3 cut(s) 198, 561, 830
ZrmI AGTACT 1 cut(s) 570
Zsp2I ATGCAT 2 cut(s) 695, 926
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.