AT3G01560

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004149
Physical Location & Seq
Forward (+)
219582 .. 221989
2408 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G01560.2

Sequence Viewer

Length: 1536 bp
ATGAATACTTGTCAGTTTATGGATAAGCAGATAATGGATCTATCTTCTTCTTCATCTCTACCTTCCACTGATTTCATCGATCTGATGAACAATCATGACGGCGATGATCACCAGAAGAAGCAGGTGATCGGCGATAATGGATTGGACTCTAAGAAAGAAGTGATCGTTCCTAGCTATGATTTCCATCCCATACGTCCCACCACCGCCGCACGATTGTCTCACTCGGCTTTAGATCTCGCCGGATCCACGACTAGGGTTAATTGGTCCGCCTCTGATTATAAACCCGTTTCCACTACTTCGCCTAACACTAATTTTGGATCTCTCGATTCTATTGAACCTTCCAAGTTGGTTCCTGACAAGGGTCAAAATGTGTTTAACACGACTATTATGTCCGAGATTATAGATAGGACAATGAAGAAACATACCGATACCTTGCTTCATGTAATGGAAGGTGTTAGTGCACGTCTTTCACAGCTGGAGACCAGAACCCACAACCTCGAGAACCTGGTGGATGATCTCAAAGTTTCTGTTGACAATAGTCACGGCAGCACTGATGGGAAAATGAGACAGCTTAAAAATATCCTTGTAGAGGTGCAGAGTGGTGTGCAACTATTGAAGGACAAACAAGAGATTCTCGAAGCTCAGCTTTCAAAGCATCAGGTATCCAACCAGCATGCCAAAACACACTCTTTACACGTTGATCCCACTGCTCAATCGCCCGCTCCAGTCCCTATGCAGCAGTTTCCTCTTACTTCTTTCCCACAGCCACCATCTTCCACTGCTGCTCCTTCCCAGCCACCTTCCTCGCAACTACCACCTCAGCTACCTACGCAGTTTTCTTCTCAACAAGAGCCGTATTGTCCTCCGCCTAGTCATCCTCAACCACCTCCATCAAACCCGCCTCCTTACCAAGCTCCTCAGACCCAAACGCCGCATCAACCATCTTACCAGTCACCGCCTCAGCAGCCACAGTACCCCCAACAGCCACCACCATCATCAGGCTACAACCCCGAAGAACAACCACCATATCAGATGCAATCTTACCCGCCAAACCCTCCTCGTCAACAGCCACCTGCTGGTTCTACCCCTTCTCAACAGTTCTACAACCCTCCTCAACCACAACCATCAATGTACGATGGAGCGGGTGGTAGATCTAATTCAGGTTTTCCGTCCGGCTACTTGTCTGAACCTTACACATACAGTGGTTCACCAATGTCCTCAGCGAAACCGCCACATATAAGTAGCAATGGAACTGGTTACCCACAGCTCTCAAATTCACGTCCATTGCCACACGCTTTGCCAATGGTTTCAGCCGTCAGCAGCGGTGGTGGCTCTAGTTCCCCTAGATCAGAAAGCAGAGCCCCAATCGATGATGTAATCGACAGGGTGACAACCATGGGATTCCCAAGGGACCAAGTCAGAGCAACAGTGAGAAAACTGACTGAAAACGGCCAAGCTGTTGACCTCAACGTGGTTCTGGATAAGTTGATGAACGAAGGAGGAGCTCCACCTGGAGGTTTTTTTGGTGGTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
DUF1421

Protein Analysis

511

Amino Acids

55.49

Weight (kDa)

6.04

Isoelectric Point (pI)

64.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 327 - 370 1.9e-20 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 279
AarI CACCTGC 2 cut(s) 112, 1081
AasI GACNNNNNNGTC 1 cut(s) 388
Acc36I ACCTGC 2 cut(s) 112, 1081
AccB7I CCANNNNNTGG 1 cut(s) 1076
AccBSI CCGCTC 2 cut(s) 722, 1142
AclWI GGATC 5 cut(s) 45, 237, 250, 325, 695
AcoI YGGCCR 1 cut(s) 1450
AcsI RAATTY 1 cut(s) 1273
AfaI GTAC 2 cut(s) 974, 1133
AfiI CCNNNNNNNGG 7 cut(s) 252, 359, 589, 998, 1076, 1471, 1514
AflIII ACRYGT 1 cut(s) 694
AgsI TTSAA 3 cut(s) 335, 616, 651
AjiI CACGTC 2 cut(s) 464, 1280
AjnI CCWGG 2 cut(s) 504, 1510
Alw21I GWGCWC 2 cut(s) 463, 1507
Alw26I GTCTC 3 cut(s) 222, 473, 559
Alw44I GTGCAC 1 cut(s) 459
AlwI GGATC 5 cut(s) 45, 237, 250, 325, 695
Ama87I CYCGRG 1 cut(s) 497
AoxI GGCC 1 cut(s) 1450
ApaLI GTGCAC 1 cut(s) 459
ApeKI GCWGC 5 cut(s) 546, 736, 782, 964, 1320
ApoI RAATTY 1 cut(s) 1273
AspS9I GGNCC 2 cut(s) 264, 1411
AsuHPI GGTGA 5 cut(s) 101, 136, 945, 1200, 1399
AvaI CYCGRG 1 cut(s) 497
AvaII GGWCC 2 cut(s) 264, 1411
BaeGI GKGCMC 1 cut(s) 463
BamHI GGATCC 1 cut(s) 242
BanII GRGCYC 2 cut(s) 1363, 1507
Bbv12I GWGCWC 2 cut(s) 463, 1507
BbvCI CCTCAGC 3 cut(s) 819, 960, 1219
BbvI GCAGC 5 cut(s) 558, 748, 769, 976, 1332
BccI CCATC 8 cut(s) 192, 548, 778, 898, 949, 1000, 1130, 1132
BceAI ACGGC 5 cut(s) 115, 559, 838, 1298, 1465
BciT130I CCWGG 2 cut(s) 506, 1512
BciVI GTATCC 1 cut(s) 673
BclI TGATCA 1 cut(s) 106
BcoDI GTCTC 3 cut(s) 222, 473, 559
BfaI CTAG 5 cut(s) 171, 252, 870, 1335, 1344
BfuAI ACCTGC 2 cut(s) 112, 1081
BfuI GTATCC 1 cut(s) 673
BglII AGATCT 2 cut(s) 232, 1151
BisI GCNGC 7 cut(s) 207, 547, 737, 783, 932, 965, 1321
BlpI GCTNAGC 1 cut(s) 642
BlsI GCNGC 7 cut(s) 208, 548, 738, 784, 933, 966, 1322
Bme1390I CCNGG 2 cut(s) 506, 1512
Bme18I GGWCC 2 cut(s) 264, 1411
BmeT110I CYCGRG 1 cut(s) 497
BmgBI CACGTC 2 cut(s) 464, 1280
BmgT120I GGNCC 2 cut(s) 264, 1411
BmiI GGNNCC 3 cut(s) 244, 351, 1412
BmrFI CCNGG 2 cut(s) 506, 1512
BmsI GCATC 3 cut(s) 664, 943, 1023
BoxI GACNNNNGTC 2 cut(s) 360, 537
BpmI CTGGAG 3 cut(s) 497, 708, 1533
Bpu10I CCTNAGC 3 cut(s) 819, 960, 1219
Bpu1102I GCTNAGC 1 cut(s) 642
Bsa29I ATCGAT 2 cut(s) 78, 1368
BsaBI GATNNNNATC 1 cut(s) 183
BsaI GGTCTC 1 cut(s) 473
BsaJI CCNNGG 2 cut(s) 1395, 1406
BsaXI ACNNNNNCTCC 2 cut(s) 769, 799
Bsc4I CCNNNNNNNGG 7 cut(s) 252, 359, 589, 998, 1076, 1471, 1514
Bse1I ACTGG 3 cut(s) 725, 949, 1258
Bse3DI GCAATG 2 cut(s) 1252, 1283
Bse8I GATNNNNATC 1 cut(s) 183
BseBI CCWGG 2 cut(s) 506, 1512
BseCI ATCGAT 2 cut(s) 78, 1368
BseDI CCNNGG 2 cut(s) 1395, 1406
BseGI GGATG 3 cut(s) 184, 517, 874
BseJI GATNNNNATC 1 cut(s) 183
BseLI CCNNNNNNNGG 7 cut(s) 252, 359, 589, 998, 1076, 1471, 1514
BseMI GCAATG 2 cut(s) 1252, 1283
BseMII CTCAG 5 cut(s) 656, 833, 932, 974, 1233
BseNI ACTGG 3 cut(s) 725, 949, 1258
BseRI GAGGAG 4 cut(s) 906, 1047, 1101, 1515
BseSI GKGCMC 1 cut(s) 463
BseXI GCAGC 5 cut(s) 558, 748, 769, 976, 1332
BseYI CCCAGC 1 cut(s) 792
BsgI GTGCAG 1 cut(s) 614
BshFI GGCC 1 cut(s) 1452
BshVI ATCGAT 2 cut(s) 78, 1368
BsiHKAI GWGCWC 2 cut(s) 463, 1507
BsiHKCI CYCGRG 1 cut(s) 497
BsiSI CCGG 2 cut(s) 240, 1173
BslFI GGGAC 3 cut(s) 180, 713, 1424
BslI CCNNNNNNNGG 7 cut(s) 252, 359, 589, 998, 1076, 1471, 1514
BsmAI GTCTC 3 cut(s) 222, 473, 559
BsmFI GGGAC 3 cut(s) 180, 713, 1424
BsnI GGCC 1 cut(s) 1452
Bso31I GGTCTC 1 cut(s) 473
BsoBI CYCGRG 1 cut(s) 497
Bsp1286I GDGCHC 3 cut(s) 463, 1363, 1507
Bsp1720I GCTNAGC 1 cut(s) 642
Bsp19I CCATGG 1 cut(s) 1395
BspANI GGCC 1 cut(s) 1452
BspCNI CTCAG 5 cut(s) 655, 832, 931, 973, 1232
BspDI ATCGAT 2 cut(s) 78, 1368
BspHI TCATGA 1 cut(s) 94
BspLI GGNNCC 3 cut(s) 244, 351, 1412
BspMI ACCTGC 2 cut(s) 112, 1081
BspPI GGATC 5 cut(s) 45, 237, 250, 325, 695
BspTNI GGTCTC 1 cut(s) 473
BsrBI CCGCTC 2 cut(s) 722, 1142
BsrDI GCAATG 2 cut(s) 1252, 1283
BsrI ACTGG 3 cut(s) 725, 949, 1258
BssECI CCNNGG 2 cut(s) 1395, 1406
BssT1I CCWWGG 2 cut(s) 1395, 1406
Bst2UI CCWGG 2 cut(s) 506, 1512
Bst4CI ACNGT 4 cut(s) 972, 1098, 1202, 1429
BstC8I GCNNGC 2 cut(s) 675, 720
BstDEI CTNAG 6 cut(s) 150, 642, 819, 918, 960, 1219
BstDSI CCRYGG 1 cut(s) 1395
BstEII GGTNACC 1 cut(s) 1256
BstENI CCTNNNNNAGG 1 cut(s) 587
BstF5I GGATG 3 cut(s) 184, 517, 874
BstMAI GTCTC 3 cut(s) 222, 473, 559
BstMWI GCNNNNNNNGC 4 cut(s) 652, 829, 964, 1329
BstNI CCWGG 2 cut(s) 506, 1512
BstNSI RCATGY 1 cut(s) 677
BstPAI GACNNNNGTC 2 cut(s) 360, 537
BstPI GGTNACC 1 cut(s) 1256
BstSCI CCNGG 2 cut(s) 504, 1510
BstSLI GKGCMC 1 cut(s) 463
BstV1I GCAGC 5 cut(s) 558, 748, 769, 976, 1332
BstX2I RGATCY 5 cut(s) 37, 232, 242, 317, 1151
BstYI RGATCY 5 cut(s) 37, 232, 242, 317, 1151
Bsu15I ATCGAT 2 cut(s) 78, 1368
BsuI GTATCC 1 cut(s) 673
BsuRI GGCC 1 cut(s) 1452
BsuTUI ATCGAT 2 cut(s) 78, 1368
BtgI CCRYGG 1 cut(s) 1395
BtgZI GCGATG 1 cut(s) 117
BtrI CACGTC 2 cut(s) 464, 1280
BtsCI GGATG 3 cut(s) 184, 517, 874
BtsI GCAGTG 2 cut(s) 705, 777
BtsIMutI CAGTG 6 cut(s) 66, 549, 705, 777, 1207, 1434
BveI ACCTGC 2 cut(s) 112, 1081
Cac8I GCNNGC 2 cut(s) 675, 720
CciI TCATGA 1 cut(s) 94
Cfr13I GGNCC 2 cut(s) 264, 1411
ClaI ATCGAT 2 cut(s) 78, 1368
CsiI ACCWGGT 1 cut(s) 504
Csp6I GTAC 2 cut(s) 973, 1132
CspCI CAANNNNNGTGG 2 cut(s) 1278, 1313
CviAII CATG 4 cut(s) 95, 440, 674, 1396
CviQI GTAC 2 cut(s) 973, 1132
DdeI CTNAG 6 cut(s) 150, 642, 819, 918, 960, 1219
DrdI GACNNNNNNGTC 1 cut(s) 388
DseDI GACNNNNNNGTC 1 cut(s) 388
EaeI YGGCCR 1 cut(s) 1450
EciI GGCGGA 2 cut(s) 256, 855
Ecl136II GAGCTC 1 cut(s) 1505
Eco130I CCWWGG 2 cut(s) 1395, 1406
Eco24I GRGCYC 2 cut(s) 1363, 1507
Eco31I GGTCTC 1 cut(s) 473
Eco47I GGWCC 2 cut(s) 264, 1411
Eco53kI GAGCTC 1 cut(s) 1505
Eco88I CYCGRG 1 cut(s) 497
Eco91I GGTNACC 1 cut(s) 1256
EcoICRI GAGCTC 1 cut(s) 1505
EcoNI CCTNNNNNAGG 1 cut(s) 587
EcoO65I GGTNACC 1 cut(s) 1256
EcoRII CCWGG 2 cut(s) 504, 1510
EcoT14I CCWWGG 2 cut(s) 1395, 1406
EcoT38I GRGCYC 2 cut(s) 1363, 1507
ErhI CCWWGG 2 cut(s) 1395, 1406
FaeI CATG 4 cut(s) 98, 443, 677, 1399
FalI AAGNNNNNCTT 2 cut(s) 630, 662
FaqI GGGAC 3 cut(s) 180, 713, 1424
FatI CATG 4 cut(s) 94, 439, 673, 1395
FauI CCCGC 4 cut(s) 727, 906, 1053, 1135
FbaI TGATCA 1 cut(s) 106
Fnu4HI GCNGC 7 cut(s) 207, 547, 737, 783, 932, 965, 1321
FokI GGATG 3 cut(s) 171, 524, 861
FriOI GRGCYC 2 cut(s) 1363, 1507
Fsp4HI GCNGC 7 cut(s) 207, 547, 737, 783, 932, 965, 1321
FspBI CTAG 5 cut(s) 171, 252, 870, 1335, 1344
GluI GCNGC 7 cut(s) 207, 547, 737, 783, 932, 965, 1321
GsaI CCCAGC 1 cut(s) 796
GsuI CTGGAG 3 cut(s) 497, 708, 1533
HaeIII GGCC 1 cut(s) 1452
HapII CCGG 2 cut(s) 240, 1173
Hin1II CATG 4 cut(s) 98, 443, 677, 1399
HincII GTYRAC 3 cut(s) 532, 1064, 1462
HindII GTYRAC 3 cut(s) 532, 1064, 1462
HinfI GANTC 4 cut(s) 146, 326, 631, 1401
HpaII CCGG 2 cut(s) 240, 1173
HphI GGTGA 5 cut(s) 101, 136, 945, 1200, 1399
Hpy166II GTNNAC 5 cut(s) 461, 532, 1064, 1208, 1462
Hpy188I TCNGA 8 cut(s) 84, 274, 394, 921, 1032, 1186, 1351, 1421
Hpy188III TCNNGA 6 cut(s) 95, 323, 353, 499, 635, 1478
Hpy8I GTNNAC 5 cut(s) 461, 532, 1064, 1208, 1462
HpyAV CCTTC 8 cut(s) 72, 348, 443, 610, 798, 810, 1098, 1490
HpyCH4III ACNGT 4 cut(s) 972, 1098, 1202, 1429
HpyCH4IV ACGT 5 cut(s) 193, 463, 696, 1279, 1470
HpyCH4V TGCA 5 cut(s) 461, 595, 607, 736, 1036
HpyF10VI GCNNNNNNNGC 4 cut(s) 652, 829, 964, 1329
HpyF3I CTNAG 6 cut(s) 150, 642, 819, 918, 960, 1219
HpySE526I ACGT 5 cut(s) 193, 463, 696, 1279, 1470
Hsp92II CATG 4 cut(s) 98, 443, 677, 1399
Ksp22I TGATCA 1 cut(s) 106
LmnI GCTCC 6 cut(s) 727, 790, 919, 1139, 1502, 1510
Lsp1109I GCAGC 5 cut(s) 558, 748, 769, 976, 1332
LweI GCATC 3 cut(s) 664, 943, 1023
MabI ACCWGGT 1 cut(s) 504
MaeI CTAG 5 cut(s) 171, 252, 870, 1335, 1344
MaeII ACGT 5 cut(s) 193, 463, 696, 1279, 1470
MaeIII GTNAC 4 cut(s) 539, 951, 1256, 1387
MbiI CCGCTC 2 cut(s) 722, 1142
MboII GAAGA 8 cut(s) 36, 39, 42, 127, 427, 765, 831, 1025
MflI RGATCY 5 cut(s) 37, 232, 242, 317, 1151
MhlI GDGCHC 3 cut(s) 463, 1363, 1507
MluCI AATT 4 cut(s) 259, 310, 1156, 1273
MlyI GAGTC 1 cut(s) 140
MmeI TCCRAC 1 cut(s) 690
MseI TTAA 3 cut(s) 258, 375, 573
MspA1I CMGCKG 2 cut(s) 475, 1323
MspI CCGG 2 cut(s) 240, 1173
MspR9I CCNGG 2 cut(s) 506, 1512
MvaI CCWGG 2 cut(s) 506, 1512
MwoI GCNNNNNNNGC 4 cut(s) 652, 829, 964, 1329
NcoI CCATGG 1 cut(s) 1395
NlaIII CATG 4 cut(s) 98, 443, 677, 1399
NlaIV GGNNCC 3 cut(s) 244, 351, 1412
NmeAIII GCCGAG 1 cut(s) 203
NmuCI GTSAC 3 cut(s) 539, 951, 1387
NspI RCATGY 1 cut(s) 677
PaeI GCATGC 1 cut(s) 677
PaeR7I CTCGAG 1 cut(s) 497
PagI TCATGA 1 cut(s) 94
PaqCI CACCTGC 2 cut(s) 112, 1081
PfeI GAWTC 3 cut(s) 326, 631, 1401
PflFI GACNNNGTC 1 cut(s) 1415
PflMI CCANNNNNTGG 1 cut(s) 1076
PkrI GCNGC 7 cut(s) 208, 548, 738, 784, 933, 966, 1322
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
PshAI GACNNNNGTC 2 cut(s) 360, 537
PsiI TTATAA 1 cut(s) 279
Psp124BI GAGCTC 1 cut(s) 1507
Psp6I CCWGG 2 cut(s) 504, 1510
PspEI GGTNACC 1 cut(s) 1256
PspFI CCCAGC 1 cut(s) 792
PspGI CCWGG 2 cut(s) 504, 1510
PspN4I GGNNCC 3 cut(s) 244, 351, 1412
PspPI GGNCC 2 cut(s) 264, 1411
PsuI RGATCY 5 cut(s) 37, 232, 242, 317, 1151
PsyI GACNNNGTC 1 cut(s) 1415
PvuII CAGCTG 1 cut(s) 475
RsaI GTAC 2 cut(s) 974, 1133
RsaNI GTAC 2 cut(s) 973, 1132
SacI GAGCTC 1 cut(s) 1507
SaqAI TTAA 3 cut(s) 258, 375, 573
SatI GCNGC 7 cut(s) 207, 547, 737, 783, 932, 965, 1321
Sau96I GGNCC 2 cut(s) 264, 1411
SchI GAGTC 1 cut(s) 140
ScrFI CCNGG 2 cut(s) 506, 1512
SduI GDGCHC 3 cut(s) 463, 1363, 1507
SexAI ACCWGGT 1 cut(s) 504
SfaNI GCATC 3 cut(s) 664, 943, 1023
Sfr274I CTCGAG 1 cut(s) 497
SinI GGWCC 2 cut(s) 264, 1411
SlaI CTCGAG 1 cut(s) 497
SmlI CTYRAG 1 cut(s) 497
SmoI CTYRAG 1 cut(s) 497
SphI GCATGC 1 cut(s) 677
Sse9I AATT 4 cut(s) 259, 310, 1156, 1273
SspMI CTAG 5 cut(s) 171, 252, 870, 1335, 1344
SstI GAGCTC 1 cut(s) 1507
StyD4I CCNGG 2 cut(s) 504, 1510
StyI CCWWGG 2 cut(s) 1395, 1406
TaaI ACNGT 4 cut(s) 972, 1098, 1202, 1429
TaiI ACGT 5 cut(s) 196, 466, 699, 1282, 1473
TaqI TCGA 6 cut(s) 78, 324, 498, 636, 1368, 1380
TasI AATT 4 cut(s) 259, 310, 1156, 1273
TauI GCSGC 2 cut(s) 209, 934
TfiI GAWTC 3 cut(s) 326, 631, 1401
Tru1I TTAA 3 cut(s) 258, 375, 573
Tru9I TTAA 3 cut(s) 258, 375, 573
TscAI CASTG 6 cut(s) 73, 556, 712, 784, 1207, 1434
TseFI GTSAC 3 cut(s) 539, 951, 1387
TseI GCWGC 5 cut(s) 546, 736, 782, 964, 1320
Tsp45I GTSAC 3 cut(s) 539, 951, 1387
TspDTI ATGAA 7 cut(s) 17, 42, 64, 101, 428, 428, 1505
TspGWI ACGGA 1 cut(s) 1158
TspRI CASTG 6 cut(s) 73, 556, 712, 784, 1207, 1434
Tth111I GACNNNGTC 1 cut(s) 1415
Van91I CCANNNNNTGG 1 cut(s) 1076
VneI GTGCAC 1 cut(s) 459
VpaK11BI GGWCC 2 cut(s) 264, 1411
XagI CCTNNNNNAGG 1 cut(s) 587
XapI RAATTY 1 cut(s) 1273
XceI RCATGY 1 cut(s) 677
XhoI CTCGAG 1 cut(s) 497
XspI CTAG 5 cut(s) 171, 252, 870, 1335, 1344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.