Rroxscaffold_2G00112390

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0004149
Physical Location & Seq
Reverse (-)
37551225 .. 37555644
4420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00112390.1

Sequence Viewer

Length: 1617 bp
ATGAATACTACATCCTTCATGGACAAGCAGATAATGGACCTGTCACAGGGATCGTCACAGCACAACAACGACTTCCTTGACCTGATGAACAATTCCCAAGAGGAAGAGCACCAGGTGGGTCATGGAAATGGGATTTCCAAGAAGGAAGAGATCTTGCCGAACTATGATTTCCAGCCCATCCGTCCCATGACTGGAGCCTCTTCGCAATCCCCAAGTTTTGATGCTACACCTAATCTCGGAGGTGGAGGTGGATCCACTAGGGCTTGGAACTCCCTAGAGTCCAAGTCCAACACCAATACTCCTATCAGAAATTATGGTTCTGTGGATTCCTTCGAACGTGCAAAAGATGTTGTGGAGAAGGACCGACATGTTCCTGATGCTACAATTATATCAGAGATTGACCACACCATGAAGAAGTATGCTGATAATTTACTTCAAGTTATGGAAGGTATTAGTGCACGACTAACACAACTTGAGAGCAGAACCCGCCACCTTGAGAATTCTGTTGATGATTTGAAGGTATCTGTGGGAAACAATCATGGAAATGCTGATGGAAAGATGAGACAAATGGAGAATATTCTTAGAGATGTGCAAACAGGTGTTCAGGATTTGAAGGATAAGCAATCAATATTAGAGGCTCAGCTGCAGCTTGGGAAGATACAGCTATCAAATCCCAAGGTAGATCCTCAATTGGAATCTCGGAATGCTCTGAATGCAGATTCTGGGCATAAAGTAGCCTCTGCTCCTCAGCACTCTCACCAACAGCTTCCTCCTGTGAACATTCCACCATCACTTCCTGCTGTTTCTCCACCAAATGCTCCTCCACAGCCTATGCTCCAAAGTGTACCACCTTCAATTCAGCATCCAAATCAGTACTCTCAGAACCAGATCCCTCCTGTTCCTCAGCAAGACCAATATTTCCCAGCTGCACCTGTTCAAACTCAAGAAGCTCCAAATCAGCAATACCAATTGCCTGCAGGTCAGCAGTCACTCCCCCTCCTATGGTACCTCCGCATCAACAATTTCAGCCTACTAGTCAACCACAGTCACCATGCTGAAGATACTCCTTATGTTCCTTCCCAGACCTACCCACCCAGTCTTCGCCAGCCACCTTCTCAGACACCTAGTGGGCCTCCTCCCTCCCAACAATATTATAGTCCAACATCCAATGTATATGAGCCACCATCTAGCAGACCTAATTCAGGGTATTCCTCTGGGTACCCTCCTTCTGGGCTTAATGAACCGTATCATTATGGTGGATCACCTTCTCAATATGGTGGTACCTCCTCAATGAAACCACAACTCTCTTCTGCTACTGCTCAAAGTCAAAGTGGAGGAAGTGGTTACCCACAGCTCCCAACAGCTAGGGTATTACCACATGCAGTGCCTACCGCCTCTGGTGTAAGTGATCGATCAGGTTCAGCTGGGACTGGAAACAAGGTTCCTATTGATGATGTGATTGAAAGAGTGACAAGCATGGGTTTTCCGAGAGACCATGTAAGGGCGACAGTTCGGAAGCTGACAGACAACGGTCAGGCAGTTGACCTGAATGTTGTGTTAGATAAGCTGATGAATGATGGGGATGTCCAGCCACCTAGAGGTTGGTTTGGTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
DUF1421

Protein Analysis

538

Amino Acids

58.65

Weight (kDa)

5.93

Isoelectric Point (pI)

59.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 483 - 526 3e-20 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 968
Acc65I GGTACC 3 cut(s) 1005, 1218, 1280
AccB1I GGYRCC 3 cut(s) 1005, 1218, 1280
AciI CCGC 3 cut(s) 487, 1012, 1392
AclWI GGATC 6 cut(s) 58, 246, 259, 677, 883, 1267
AcsI RAATTY 1 cut(s) 499
AcuI CTGAAG 1 cut(s) 1077
AdeI CACNNNGTG 2 cut(s) 115, 1127
AfaI GTAC 5 cut(s) 846, 875, 1007, 1220, 1282
AfiI CCNNNNNNNGG 8 cut(s) 46, 191, 236, 1002, 1202, 1229, 1501, 1598
AflIII ACRYGT 1 cut(s) 367
AgsI TTSAA 6 cut(s) 437, 517, 613, 855, 938, 1463
AhlI ACTAGT 1 cut(s) 1033
AjnI CCWGG 1 cut(s) 111
AloI GAACNNNNNNTCC 2 cut(s) 1425, 1457
Alw21I GWGCWC 2 cut(s) 111, 460
Alw26I GTCTC 2 cut(s) 556, 1485
Alw44I GTGCAC 1 cut(s) 456
AlwI GGATC 6 cut(s) 58, 246, 259, 677, 883, 1267
AlwNI CAGNNNCTG 1 cut(s) 722
AoxI GGCC 1 cut(s) 1130
ApaLI GTGCAC 1 cut(s) 456
ApeKI GCWGC 3 cut(s) 643, 646, 926
ApoI RAATTY 1 cut(s) 499
Asp718I GGTACC 3 cut(s) 1005, 1218, 1280
AspS9I GGNCC 3 cut(s) 37, 361, 1130
AsuHPI GGTGA 3 cut(s) 749, 1040, 1254
AsuII TTCGAA 1 cut(s) 333
AvaII GGWCC 2 cut(s) 37, 361
BaeGI GKGCMC 1 cut(s) 460
BamHI GGATCC 1 cut(s) 251
BanI GGYRCC 3 cut(s) 1005, 1218, 1280
BbsI GAAGAC 1 cut(s) 1091
Bbv12I GWGCWC 2 cut(s) 111, 460
BbvCI CCTCAGC 2 cut(s) 747, 903
BbvI GCAGC 3 cut(s) 630, 658, 913
BccI CCATC 5 cut(s) 185, 545, 796, 1192, 1571
BciT130I CCWGG 1 cut(s) 113
BcoDI GTCTC 2 cut(s) 556, 1485
BcuI ACTAGT 1 cut(s) 1033
BfaI CTAG 7 cut(s) 258, 275, 1034, 1125, 1188, 1365, 1596
BfmI CTRYAG 2 cut(s) 644, 975
BfuAI ACCTGC 1 cut(s) 968
BglII AGATCT 1 cut(s) 150
BisI GCNGC 3 cut(s) 644, 647, 927
BlpI GCTNAGC 1 cut(s) 639
BlsI GCNGC 3 cut(s) 645, 648, 928
BmcAI AGTACT 1 cut(s) 875
Bme1390I CCNGG 1 cut(s) 113
Bme18I GGWCC 2 cut(s) 37, 361
BmgT120I GGNCC 3 cut(s) 37, 361, 1130
BmiI GGNNCC 6 cut(s) 196, 253, 1007, 1220, 1282, 1443
BmrFI CCNGG 1 cut(s) 113
BmrI ACTGGG 1 cut(s) 1089
BmsI GCATC 4 cut(s) 211, 367, 871, 1023
BmuI ACTGGG 1 cut(s) 1089
BoxI GACNNNNGTC 1 cut(s) 1530
BpiI GAAGAC 1 cut(s) 1091
BpmI CTGGAG 1 cut(s) 213
Bpu10I CCTNAGC 2 cut(s) 747, 903
Bpu1102I GCTNAGC 1 cut(s) 639
Bpu14I TTCGAA 1 cut(s) 333
BpuEI CTTGAG 3 cut(s) 494, 515, 927
Bsa29I ATCGAT 1 cut(s) 1411
BsaI GGTCTC 1 cut(s) 1485
BsaJI CCNNGG 1 cut(s) 675
BsaXI ACNNNNNCTCC 4 cut(s) 283, 313, 981, 1011
Bsc4I CCNNNNNNNGG 8 cut(s) 46, 191, 236, 1002, 1202, 1229, 1501, 1598
Bse1I ACTGG 3 cut(s) 196, 1095, 1435
BseBI CCWGG 1 cut(s) 113
BseCI ATCGAT 1 cut(s) 1411
BseDI CCNNGG 1 cut(s) 675
BseGI GGATG 5 cut(s) 11, 177, 862, 1163, 1588
BseLI CCNNNNNNNGG 8 cut(s) 46, 191, 236, 1002, 1202, 1229, 1501, 1598
BseMII CTCAG 5 cut(s) 653, 761, 893, 917, 1130
BseNI ACTGG 3 cut(s) 196, 1095, 1435
BseRI GAGGAG 4 cut(s) 735, 810, 1125, 1276
BseSI GKGCMC 1 cut(s) 460
BseXI GCAGC 3 cut(s) 630, 658, 913
BseYI CCCAGC 2 cut(s) 922, 1424
BsgI GTGCAG 1 cut(s) 912
BshFI GGCC 1 cut(s) 1132
BshNI GGYRCC 3 cut(s) 1005, 1218, 1280
BshVI ATCGAT 1 cut(s) 1411
BsiHKAI GWGCWC 2 cut(s) 111, 460
BslFI GGGAC 2 cut(s) 168, 1441
BslI CCNNNNNNNGG 8 cut(s) 46, 191, 236, 1002, 1202, 1229, 1501, 1598
BsmAI GTCTC 2 cut(s) 556, 1485
BsmFI GGGAC 2 cut(s) 168, 1441
BsmI GAATGC 2 cut(s) 709, 718
BsnI GGCC 1 cut(s) 1132
Bso31I GGTCTC 1 cut(s) 1485
Bsp119I TTCGAA 1 cut(s) 333
Bsp1286I GDGCHC 2 cut(s) 111, 460
Bsp143I GATC 8 cut(s) 50, 150, 251, 682, 888, 1259, 1408, 1412
Bsp1720I GCTNAGC 1 cut(s) 639
BspACI CCGC 3 cut(s) 487, 1012, 1392
BspANI GGCC 1 cut(s) 1132
BspCNI CTCAG 5 cut(s) 652, 760, 892, 916, 1129
BspDI ATCGAT 1 cut(s) 1411
BspLI GGNNCC 6 cut(s) 196, 253, 1007, 1220, 1282, 1443
BspMAI CTGCAG 2 cut(s) 648, 979
BspMI ACCTGC 1 cut(s) 968
BspPI GGATC 6 cut(s) 58, 246, 259, 677, 883, 1267
BspQI GCTCTTC 1 cut(s) 99
BspT104I TTCGAA 1 cut(s) 333
BspT107I GGYRCC 3 cut(s) 1005, 1218, 1280
BspTNI GGTCTC 1 cut(s) 1485
BsrI ACTGG 3 cut(s) 196, 1095, 1435
BssECI CCNNGG 1 cut(s) 675
BssMI GATC 8 cut(s) 50, 150, 251, 682, 888, 1259, 1408, 1412
BssT1I CCWWGG 1 cut(s) 675
Bst2UI CCWGG 1 cut(s) 113
Bst4CI ACNGT 4 cut(s) 1046, 1245, 1510, 1532
Bst6I CTCTTC 4 cut(s) 99, 141, 205, 1312
BstBI TTCGAA 1 cut(s) 333
BstC8I GCNNGC 2 cut(s) 975, 1106
BstDEI CTNAG 6 cut(s) 581, 639, 747, 879, 903, 1116
BstEII GGTNACC 1 cut(s) 1343
BstENI CCTNNNNNAGG 2 cut(s) 44, 1200
BstF5I GGATG 5 cut(s) 11, 177, 862, 1163, 1588
BstKTI GATC 8 cut(s) 53, 153, 254, 685, 891, 1262, 1411, 1415
BstMAI GTCTC 2 cut(s) 556, 1485
BstMBI GATC 8 cut(s) 50, 150, 251, 682, 888, 1259, 1408, 1412
BstMWI GCNNNNNNNGC 2 cut(s) 486, 713
BstNI CCWGG 1 cut(s) 113
BstNSI RCATGY 2 cut(s) 371, 1382
BstPAI GACNNNNGTC 1 cut(s) 1530
BstPI GGTNACC 1 cut(s) 1343
BstSCI CCNGG 1 cut(s) 111
BstSFI CTRYAG 2 cut(s) 644, 975
BstSLI GKGCMC 1 cut(s) 460
BstV1I GCAGC 3 cut(s) 630, 658, 913
BstV2I GAAGAC 1 cut(s) 1091
BstX2I RGATCY 4 cut(s) 150, 251, 682, 888
BstYI RGATCY 4 cut(s) 150, 251, 682, 888
Bsu15I ATCGAT 1 cut(s) 1411
BsuRI GGCC 1 cut(s) 1132
BsuTUI ATCGAT 1 cut(s) 1411
BtsCI GGATG 5 cut(s) 11, 177, 862, 1163, 1588
BtsI GCAGTG 1 cut(s) 1389
BtsIMutI CAGTG 1 cut(s) 1389
BveI ACCTGC 1 cut(s) 968
Cac8I GCNNGC 2 cut(s) 975, 1106
CaiI CAGNNNCTG 1 cut(s) 722
Cfr13I GGNCC 3 cut(s) 37, 361, 1130
ClaI ATCGAT 1 cut(s) 1411
CsiI ACCWGGT 1 cut(s) 111
Csp6I GTAC 5 cut(s) 845, 874, 1006, 1219, 1281
CviQI GTAC 5 cut(s) 845, 874, 1006, 1219, 1281
DdeI CTNAG 6 cut(s) 581, 639, 747, 879, 903, 1116
DpnI GATC 8 cut(s) 52, 152, 253, 684, 890, 1261, 1410, 1414
DpnII GATC 8 cut(s) 50, 150, 251, 682, 888, 1259, 1408, 1412
DraIII CACNNNGTG 2 cut(s) 115, 1127
Eam1104I CTCTTC 4 cut(s) 99, 141, 205, 1312
EarI CTCTTC 4 cut(s) 99, 141, 205, 1312
Eco130I CCWWGG 1 cut(s) 675
Eco31I GGTCTC 1 cut(s) 1485
Eco47I GGWCC 2 cut(s) 37, 361
Eco57I CTGAAG 1 cut(s) 1077
Eco91I GGTNACC 1 cut(s) 1343
EcoNI CCTNNNNNAGG 2 cut(s) 44, 1200
EcoO65I GGTNACC 1 cut(s) 1343
EcoRI GAATTC 1 cut(s) 499
EcoRII CCWGG 1 cut(s) 111
EcoT14I CCWWGG 1 cut(s) 675
ErhI CCWWGG 1 cut(s) 675
FaqI GGGAC 2 cut(s) 168, 1441
FauI CCCGC 1 cut(s) 494
Fnu4HI GCNGC 3 cut(s) 644, 647, 927
FokI GGATG 4 cut(s) 164, 849, 1150, 1595
Fsp4HI GCNGC 3 cut(s) 644, 647, 927
FspBI CTAG 7 cut(s) 258, 275, 1034, 1125, 1188, 1365, 1596
GluI GCNGC 3 cut(s) 644, 647, 927
GsaI CCCAGC 2 cut(s) 926, 1428
GsuI CTGGAG 1 cut(s) 213
HaeIII GGCC 1 cut(s) 1132
HincII GTYRAC 2 cut(s) 1039, 1543
HindII GTYRAC 2 cut(s) 1039, 1543
HinfI GANTC 4 cut(s) 278, 326, 695, 719
HphI GGTGA 3 cut(s) 749, 1040, 1254
Hpy166II GTNNAC 5 cut(s) 458, 778, 845, 1039, 1543
Hpy188I TCNGA 9 cut(s) 239, 308, 394, 702, 711, 882, 1119, 1488, 1515
Hpy188III TCNNGA 3 cut(s) 374, 605, 944
Hpy8I GTNNAC 5 cut(s) 458, 778, 845, 1039, 1543
HpyCH4III ACNGT 4 cut(s) 1046, 1245, 1510, 1532
HpyCH4IV ACGT 1 cut(s) 337
HpyCH4V TGCA 8 cut(s) 341, 458, 592, 646, 716, 929, 977, 1382
HpyF10VI GCNNNNNNNGC 2 cut(s) 486, 713
HpyF3I CTNAG 6 cut(s) 581, 639, 747, 879, 903, 1116
HpySE526I ACGT 1 cut(s) 337
KpnI GGTACC 3 cut(s) 1009, 1222, 1284
Kzo9I GATC 8 cut(s) 50, 150, 251, 682, 888, 1259, 1408, 1412
LguI GCTCTTC 1 cut(s) 99
LmnI GCTCC 6 cut(s) 194, 748, 823, 840, 955, 1359
Lsp1109I GCAGC 3 cut(s) 630, 658, 913
LweI GCATC 4 cut(s) 211, 367, 871, 1023
MabI ACCWGGT 1 cut(s) 111
MaeI CTAG 7 cut(s) 258, 275, 1034, 1125, 1188, 1365, 1596
MaeII ACGT 1 cut(s) 337
MaeIII GTNAC 6 cut(s) 42, 54, 987, 1046, 1343, 1468
MalI GATC 8 cut(s) 52, 152, 253, 684, 890, 1261, 1410, 1414
MboI GATC 8 cut(s) 50, 150, 251, 682, 888, 1259, 1408, 1412
MboII GAAGA 8 cut(s) 116, 158, 192, 424, 667, 1070, 1091, 1299
MfeI CAATTG 2 cut(s) 689, 968
MflI RGATCY 4 cut(s) 150, 251, 682, 888
MhlI GDGCHC 2 cut(s) 111, 460
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 2 cut(s) 312, 1184
MseI TTAA 1 cut(s) 1236
MslI CAYNNNNRTG 3 cut(s) 126, 543, 1254
MspA1I CMGCKG 3 cut(s) 643, 926, 1424
MspR9I CCNGG 1 cut(s) 113
MunI CAATTG 2 cut(s) 689, 968
Mva1269I GAATGC 2 cut(s) 709, 718
MvaI CCWGG 1 cut(s) 113
MwoI GCNNNNNNNGC 2 cut(s) 486, 713
NdeII GATC 8 cut(s) 50, 150, 251, 682, 888, 1259, 1408, 1412
NlaIV GGNNCC 6 cut(s) 196, 253, 1007, 1220, 1282, 1443
NmuCI GTSAC 5 cut(s) 42, 54, 987, 1046, 1468
NspI RCATGY 2 cut(s) 371, 1382
NspV TTCGAA 1 cut(s) 333
PciI ACATGT 1 cut(s) 367
PciSI GCTCTTC 1 cut(s) 99
PctI GAATGC 2 cut(s) 709, 718
PfeI GAWTC 3 cut(s) 326, 695, 719
PkrI GCNGC 3 cut(s) 645, 648, 928
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
PscI ACATGT 1 cut(s) 367
PshAI GACNNNNGTC 1 cut(s) 1530
Psp6I CCWGG 1 cut(s) 111
PspEI GGTNACC 1 cut(s) 1343
PspFI CCCAGC 2 cut(s) 922, 1424
PspGI CCWGG 1 cut(s) 111
PspN4I GGNNCC 6 cut(s) 196, 253, 1007, 1220, 1282, 1443
PspPI GGNCC 3 cut(s) 37, 361, 1130
PstI CTGCAG 2 cut(s) 648, 979
PstNI CAGNNNCTG 1 cut(s) 722
PsuI RGATCY 4 cut(s) 150, 251, 682, 888
PvuII CAGCTG 3 cut(s) 643, 926, 1424
RsaI GTAC 5 cut(s) 846, 875, 1007, 1220, 1282
RsaNI GTAC 5 cut(s) 845, 874, 1006, 1219, 1281
RseI CAYNNNNRTG 3 cut(s) 126, 543, 1254
SapI GCTCTTC 1 cut(s) 99
SaqAI TTAA 1 cut(s) 1236
SatI GCNGC 3 cut(s) 644, 647, 927
Sau3AI GATC 8 cut(s) 50, 150, 251, 682, 888, 1259, 1408, 1412
Sau96I GGNCC 3 cut(s) 37, 361, 1130
SbfI CCTGCAGG 1 cut(s) 979
ScaI AGTACT 1 cut(s) 875
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 1 cut(s) 113
SdaI CCTGCAGG 1 cut(s) 979
SduI GDGCHC 2 cut(s) 111, 460
SexAI ACCWGGT 1 cut(s) 111
SfaNI GCATC 4 cut(s) 211, 367, 871, 1023
SfcI CTRYAG 2 cut(s) 644, 975
SfuI TTCGAA 1 cut(s) 333
SinI GGWCC 2 cut(s) 37, 361
SmiMI CAYNNNNRTG 3 cut(s) 126, 543, 1254
SmlI CTYRAG 3 cut(s) 473, 494, 942
SmoI CTYRAG 3 cut(s) 473, 494, 942
SpeI ACTAGT 1 cut(s) 1033
Sse8387I CCTGCAGG 1 cut(s) 979
SsiI CCGC 3 cut(s) 487, 1012, 1392
SspI AATATT 4 cut(s) 577, 630, 917, 1151
SspMI CTAG 7 cut(s) 258, 275, 1034, 1125, 1188, 1365, 1596
StyD4I CCNGG 1 cut(s) 111
StyI CCWWGG 1 cut(s) 675
TaaI ACNGT 4 cut(s) 1046, 1245, 1510, 1532
TaiI ACGT 1 cut(s) 340
TaqI TCGA 2 cut(s) 333, 1411
TaqII GACCGA 1 cut(s) 378
TatI WGTACW 1 cut(s) 873
TfiI GAWTC 3 cut(s) 326, 695, 719
Tru1I TTAA 1 cut(s) 1236
Tru9I TTAA 1 cut(s) 1236
TscAI CASTG 1 cut(s) 1389
TseFI GTSAC 5 cut(s) 42, 54, 987, 1046, 1468
TseI GCWGC 3 cut(s) 643, 646, 926
Tsp45I GTSAC 5 cut(s) 42, 54, 987, 1046, 1468
TspDTI ATGAA 7 cut(s) 7, 17, 101, 425, 1254, 1307, 1586
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 1 cut(s) 1389
VneI GTGCAC 1 cut(s) 456
VpaK11BI GGWCC 2 cut(s) 37, 361
XagI CCTNNNNNAGG 2 cut(s) 44, 1200
XapI RAATTY 1 cut(s) 499
XceI RCATGY 2 cut(s) 371, 1382
XcmI CCANNNNNNNNNTGG 2 cut(s) 119, 1599
XspI CTAG 7 cut(s) 258, 275, 1034, 1125, 1188, 1365, 1596
ZrmI AGTACT 1 cut(s) 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.