AT5G14540

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004149
Physical Location & Seq
Reverse (-)
4687069 .. 4689923
2855 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G14540.1

Sequence Viewer

Length: 1644 bp
ATGCAATCTTTCGATCTAATAAAATCGGCCCTGTTCTCGGATAAGCAGATAATGGATCTCATGAACGACAACAGCAACAATTCACAAGACGGCGATCATCAGAACTACCGTGTCGGCGATAATGGATTGGAATCGAAGAAGGAAGCTATCTTCCCTAGCTATGATTTCCAACCTATGCGCCCTAATGCCTCCGCCGGATTGTCTCACCACGCTTTAGATCTCGCCGGATCTGTTAATTCCACGGCTGCTAGGGTTTGGGATGCGTCTGATCCTAAACCGGTTTCAGCTTCTTCCGCAAGAAGTTATGGCTCTATGGACTCTCTCGAACCTTCAAAGTTGTTTGCTGAGAAGGATCGAAATTCCCCCGAGTCAGCGATTATATCTGCGATCGATCGGACAATGAAGGCCCATGCTGATAAATTGTTACATGTTATGGAAGGTGTCAGTGCACGTCTAACGCAGCTGGAGACCAGAACCCGAGATCTCGAGAATTTGGTTGATGATGTGAAAGTTTCTGTTGGGAACAGTCATGGAAAAACTGATGGTAAATTGAGACAGCTTGAAAACATCATGTTAGAGGTGCAAAACGGTGTACAGTTGTTGAAGGACAAACAAGAAATAGTTGAGGCGCAGCTTCAACTTTCAAAGCTCCAGTTATCAAAGGTAAACCAGCAGCCGGAGACACACTCTACACATGTTGAACCCACTGCTCAGCCGCCTGCATCTCTGCCTCAGCCACCAGCTTCTGCTGCAGCTCCCCCGTCTCTTACTCAGCAGGGCCTCCCACCACAACAATTTATCCAACCGCCTGCCTCACAGCACGGTCTCTCACCACCTTCATTACAACTGCCTCAGCTTCCTAACCAGTTCTCTCCCCAACAAGAGCCTTATTTCCCTCCGTCTGGTCAGTCCCAGCCACCTCCAACAATCCAGCCTCCTTATCAACCTCCTCCACCAACCCAGTCATTGCATCAACCCCCTTACCAGCCACCTCCTCAACAGCCACAATACCCGCAACAGCCGCCTCCTCAGCTCCAACACCCGTCAGGCTACAACCCGGAGGAACCACCTTACCCTCAGCAATCTTACCCGCCAAACCCTCCTCGTCAACCACCTTCTCATCCACCTCCAGGTTCTGCCCCATCTCAACAGTACTACAACGCTCCTCCAACGCCACCATCAATGTATGATGGACCAGGCGGCAGATCCAATTCAGGTTTTCCTTCCGGATATTCTCCTGAATCATACCCGTATACCGGGCCACCTTCACAGTATGGAAACACCCCATCTGTGAAACCCACACATCAGAGTGGAAGTGGATCTGGTGCCTACCCACAGCTCCCAATGGCACGCCCACTACCACAAGGTTTACCAATGGCATCAGCCATCAGCAGCGGTGGAAGTGGTGGTGGCTCAGATTCCCCAAGATCAGGAAACAGAGCCCCAGTCGATGATGTCATTGACAAAGTGGTTAGCATGGGGTTCCCAAGGGACCAAGTGAGAGGAACAGTGAGAACATTGACAGAGAACGGTCAAGCTGTTGACCTCAATGTTGTTTTAGATAAGCTGATGAATGGAGATAGAGGAGCTATGATGCAGCAGCAGCAGCAGCAACCGCCAAGAGGTTGGTTTGGTGGTCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
DUF1421

Protein Analysis

547

Amino Acids

59.1

Weight (kDa)

5.91

Isoelectric Point (pI)

62.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 483 - 526 9.3e-22 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1325
AccI GTMKAC 1 cut(s) 1253
AccIII TCCGGA 1 cut(s) 1226
AclWI GGATC 6 cut(s) 63, 235, 263, 360, 1200, 1327
AcsI RAATTY 2 cut(s) 358, 490
AfaI GTAC 2 cut(s) 594, 1154
AfiI CCNNNNNNNGG 7 cut(s) 37, 676, 902, 1130, 1256, 1430, 1622
AflIII ACRYGT 2 cut(s) 427, 694
AgeI ACCGGT 1 cut(s) 277
AgsI TTSAA 6 cut(s) 333, 563, 604, 638, 645, 701
AjiI CACGTC 1 cut(s) 452
AjnI CCWGG 2 cut(s) 1129, 1195
Alw21I GWGCWC 1 cut(s) 451
Alw26I GTCTC 6 cut(s) 207, 461, 547, 674, 768, 830
Alw44I GTGCAC 1 cut(s) 447
AlwI GGATC 6 cut(s) 63, 235, 263, 360, 1200, 1327
AlwNI CAGNNNCTG 2 cut(s) 746, 1136
Ama87I CYCGRG 3 cut(s) 365, 477, 485
Aor13HI TCCGGA 1 cut(s) 1226
AoxI GGCC 4 cut(s) 27, 405, 778, 1259
ApaLI GTGCAC 1 cut(s) 447
ApoI RAATTY 2 cut(s) 358, 490
AsiGI ACCGGT 1 cut(s) 277
AspLEI GCGC 2 cut(s) 180, 631
AspS9I GGNCC 6 cut(s) 28, 406, 778, 1193, 1259, 1492
AsuC2I CCSGG 2 cut(s) 1058, 1258
AsuHPI GGTGA 2 cut(s) 197, 822
AvaI CYCGRG 3 cut(s) 365, 477, 485
AvaII GGWCC 2 cut(s) 1193, 1492
BaeGI GKGCMC 1 cut(s) 451
BanI GGYRCC 1 cut(s) 1325
BanII GRGCYC 1 cut(s) 1444
Bbv12I GWGCWC 1 cut(s) 451
BbvCI CCTCAGC 4 cut(s) 732, 852, 1029, 1077
BccI CCATC 6 cut(s) 536, 1150, 1184, 1186, 1294, 1394
BceAI ACGGC 2 cut(s) 106, 258
BciT130I CCWGG 2 cut(s) 1131, 1197
BcnI CCSGG 2 cut(s) 1058, 1258
BcoDI GTCTC 6 cut(s) 207, 461, 547, 674, 768, 830
BfaI CTAG 2 cut(s) 156, 249
BfmI CTRYAG 1 cut(s) 750
BglII AGATCT 2 cut(s) 217, 481
BlpI GCTNAGC 1 cut(s) 711
BmcAI AGTACT 1 cut(s) 1154
Bme1390I CCNGG 4 cut(s) 1058, 1131, 1197, 1258
Bme18I GGWCC 2 cut(s) 1193, 1492
BmeT110I CYCGRG 3 cut(s) 365, 477, 485
BmgBI CACGTC 1 cut(s) 452
BmgT120I GGNCC 6 cut(s) 28, 406, 778, 1193, 1259, 1492
BmiI GGNNCC 4 cut(s) 1065, 1327, 1484, 1493
BmrFI CCNGG 4 cut(s) 1058, 1131, 1197, 1258
BmrI ACTGGG 2 cut(s) 955, 1439
BmsI GCATC 5 cut(s) 250, 731, 979, 1388, 1584
BmuI ACTGGG 2 cut(s) 955, 1439
BplI GAGNNNNNCTC 2 cut(s) 671, 703
BpmI CTGGAG 3 cut(s) 485, 635, 1113
Bpu10I CCTNAGC 4 cut(s) 732, 852, 1029, 1077
Bpu1102I GCTNAGC 1 cut(s) 711
BpuMI CCSGG 2 cut(s) 1058, 1258
Bsa29I ATCGAT 1 cut(s) 390
BsaBI GATNNNNATC 1 cut(s) 130
BsaI GGTCTC 2 cut(s) 461, 830
BsaJI CCNNGG 2 cut(s) 240, 1487
BsaWI WCCGGW 2 cut(s) 277, 1226
Bsc4I CCNNNNNNNGG 7 cut(s) 37, 676, 902, 1130, 1256, 1430, 1622
Bse118I RCCGGY 1 cut(s) 277
Bse1I ACTGG 4 cut(s) 652, 865, 961, 1445
Bse3DI GCAATG 1 cut(s) 965
Bse8I GATNNNNATC 1 cut(s) 130
BseAI TCCGGA 1 cut(s) 1226
BseBI CCWGG 2 cut(s) 1131, 1197
BseCI ATCGAT 1 cut(s) 390
BseDI CCNNGG 2 cut(s) 240, 1487
BseGI GGATG 2 cut(s) 265, 1120
BseJI GATNNNNATC 1 cut(s) 130
BseLI CCNNNNNNNGG 7 cut(s) 37, 676, 902, 1130, 1256, 1430, 1622
BseMI GCAATG 1 cut(s) 965
BseMII CTCAG 8 cut(s) 336, 725, 746, 785, 866, 1043, 1091, 1428
BseNI ACTGG 4 cut(s) 652, 865, 961, 1445
BseRI GAGGAG 6 cut(s) 939, 984, 1017, 1092, 1155, 1599
BseSI GKGCMC 1 cut(s) 451
BseYI CCCAGC 1 cut(s) 912
Bsh1285I CGRYCG 2 cut(s) 390, 394
BshFI GGCC 4 cut(s) 29, 407, 780, 1261
BshNI GGYRCC 1 cut(s) 1325
BshTI ACCGGT 1 cut(s) 277
BshVI ATCGAT 1 cut(s) 390
BsiEI CGRYCG 2 cut(s) 390, 394
BsiHKAI GWGCWC 1 cut(s) 451
BsiHKCI CYCGRG 3 cut(s) 365, 477, 485
BsiSI CCGG 7 cut(s) 195, 225, 278, 677, 1058, 1227, 1257
BslFI GGGAC 2 cut(s) 895, 1505
BslI CCNNNNNNNGG 7 cut(s) 37, 676, 902, 1130, 1256, 1430, 1622
BsmAI GTCTC 6 cut(s) 207, 461, 547, 674, 768, 830
BsmBI CGTCTC 1 cut(s) 768
BsmFI GGGAC 2 cut(s) 895, 1505
BsnI GGCC 4 cut(s) 29, 407, 780, 1261
Bso31I GGTCTC 2 cut(s) 461, 830
BsoBI CYCGRG 3 cut(s) 365, 477, 485
Bsp1286I GDGCHC 2 cut(s) 451, 1444
Bsp13I TCCGGA 1 cut(s) 1226
Bsp1407I TGTACA 1 cut(s) 592
Bsp1720I GCTNAGC 1 cut(s) 711
BspANI GGCC 4 cut(s) 29, 407, 780, 1261
BspCNI CTCAG 8 cut(s) 337, 724, 745, 784, 865, 1042, 1090, 1427
BspDI ATCGAT 1 cut(s) 390
BspEI TCCGGA 1 cut(s) 1226
BspHI TCATGA 1 cut(s) 60
BspLI GGNNCC 4 cut(s) 1065, 1327, 1484, 1493
BspMAI CTGCAG 1 cut(s) 754
BspPI GGATC 6 cut(s) 63, 235, 263, 360, 1200, 1327
BspT107I GGYRCC 1 cut(s) 1325
BspTNI GGTCTC 2 cut(s) 461, 830
BsrDI GCAATG 1 cut(s) 965
BsrFI RCCGGY 1 cut(s) 277
BsrGI TGTACA 1 cut(s) 592
BsrI ACTGG 4 cut(s) 652, 865, 961, 1445
BssAI RCCGGY 1 cut(s) 277
BssECI CCNNGG 2 cut(s) 240, 1487
BssNAI GTATAC 1 cut(s) 1254
BssT1I CCWWGG 1 cut(s) 1487
Bst1107I GTATAC 1 cut(s) 1254
Bst2UI CCWGG 2 cut(s) 1131, 1197
Bst4CI ACNGT 9 cut(s) 110, 527, 590, 597, 824, 1152, 1272, 1510, 1532
BstAUI TGTACA 1 cut(s) 592
BstC8I GCNNGC 3 cut(s) 720, 810, 1351
BstDEI CTNAG 8 cut(s) 345, 711, 732, 771, 852, 1029, 1077, 1414
BstDSI CCRYGG 1 cut(s) 240
BstF5I GGATG 2 cut(s) 265, 1120
BstHHI GCGC 2 cut(s) 180, 631
BstMAI GTCTC 6 cut(s) 207, 461, 547, 674, 768, 830
BstMCI CGRYCG 2 cut(s) 390, 394
BstMWI GCNNNNNNNGC 8 cut(s) 293, 749, 1021, 1030, 1603, 1606, 1609, 1615
BstNI CCWGG 2 cut(s) 1131, 1197
BstNSI RCATGY 2 cut(s) 431, 698
BstSCI CCNGG 4 cut(s) 1056, 1129, 1195, 1256
BstSFI CTRYAG 1 cut(s) 750
BstSLI GKGCMC 1 cut(s) 451
BstX2I RGATCY 6 cut(s) 55, 217, 227, 481, 1205, 1319
BstXI CCANNNNNNTGG 1 cut(s) 1626
BstYI RGATCY 6 cut(s) 55, 217, 227, 481, 1205, 1319
BstZ17I GTATAC 1 cut(s) 1254
Bsu15I ATCGAT 1 cut(s) 390
BsuRI GGCC 4 cut(s) 29, 407, 780, 1261
BsuTUI ATCGAT 1 cut(s) 390
BtgI CCRYGG 1 cut(s) 240
BtrI CACGTC 1 cut(s) 452
BtsCI GGATG 2 cut(s) 265, 1120
BtsI GCAGTG 1 cut(s) 705
BtsIMutI CAGTG 3 cut(s) 451, 705, 1515
Cac8I GCNNGC 3 cut(s) 720, 810, 1351
CaiI CAGNNNCTG 2 cut(s) 746, 1136
CciI TCATGA 1 cut(s) 60
CfoI GCGC 2 cut(s) 180, 631
Cfr10I RCCGGY 1 cut(s) 277
Cfr13I GGNCC 6 cut(s) 28, 406, 778, 1193, 1259, 1492
ClaI ATCGAT 1 cut(s) 390
CseI GACGC 1 cut(s) 252
Csp6I GTAC 2 cut(s) 593, 1153
CspAI ACCGGT 1 cut(s) 277
CviAII CATG 7 cut(s) 61, 410, 428, 530, 571, 695, 1477
CviQI GTAC 2 cut(s) 593, 1153
DdeI CTNAG 8 cut(s) 345, 711, 732, 771, 852, 1029, 1077, 1414
EciI GGCGGA 1 cut(s) 181
Eco130I CCWWGG 1 cut(s) 1487
Eco24I GRGCYC 1 cut(s) 1444
Eco31I GGTCTC 2 cut(s) 461, 830
Eco47I GGWCC 2 cut(s) 1193, 1492
Eco88I CYCGRG 3 cut(s) 365, 477, 485
EcoO109I RGGNCCY 1 cut(s) 778
EcoRII CCWGG 2 cut(s) 1129, 1195
EcoT14I CCWWGG 1 cut(s) 1487
EcoT38I GRGCYC 1 cut(s) 1444
ErhI CCWWGG 1 cut(s) 1487
Esp3I CGTCTC 1 cut(s) 768
FaeI CATG 7 cut(s) 64, 413, 431, 533, 574, 698, 1480
FaqI GGGAC 2 cut(s) 895, 1505
FatI CATG 7 cut(s) 60, 409, 427, 529, 570, 694, 1476
FauI CCCGC 2 cut(s) 1020, 1098
FblI GTMKAC 1 cut(s) 1253
FokI GGATG 2 cut(s) 272, 1107
FriOI GRGCYC 1 cut(s) 1444
FspBI CTAG 2 cut(s) 156, 249
GlaI GCGC 2 cut(s) 179, 630
GsaI CCCAGC 1 cut(s) 916
GsuI CTGGAG 3 cut(s) 485, 635, 1113
HaeIII GGCC 4 cut(s) 29, 407, 780, 1261
HapII CCGG 7 cut(s) 195, 225, 278, 677, 1058, 1227, 1257
HgaI GACGC 1 cut(s) 252
HhaI GCGC 2 cut(s) 180, 631
Hin1II CATG 7 cut(s) 64, 413, 431, 533, 574, 698, 1480
Hin6I GCGC 2 cut(s) 178, 629
HinP1I GCGC 2 cut(s) 178, 629
HincII GTYRAC 2 cut(s) 1109, 1543
HindII GTYRAC 2 cut(s) 1109, 1543
HinfI GANTC 5 cut(s) 131, 317, 368, 1241, 1418
HpaII CCGG 7 cut(s) 195, 225, 278, 677, 1058, 1227, 1257
HphI GGTGA 2 cut(s) 197, 822
Hpy166II GTNNAC 7 cut(s) 449, 593, 667, 1109, 1254, 1370, 1543
Hpy188I TCNGA 6 cut(s) 40, 102, 268, 396, 1308, 1417
Hpy188III TCNNGA 7 cut(s) 61, 323, 485, 487, 1227, 1238, 1431
Hpy8I GTNNAC 7 cut(s) 449, 593, 667, 1109, 1254, 1370, 1543
HpyCH4III ACNGT 9 cut(s) 110, 527, 590, 597, 824, 1152, 1272, 1510, 1532
HpyCH4IV ACGT 1 cut(s) 451
HpyCH4V TGCA 7 cut(s) 4, 449, 583, 722, 752, 970, 1597
HpyF10VI GCNNNNNNNGC 8 cut(s) 293, 749, 1021, 1030, 1603, 1606, 1609, 1615
HpyF3I CTNAG 8 cut(s) 345, 711, 732, 771, 852, 1029, 1077, 1414
HpySE526I ACGT 1 cut(s) 451
Hsp92II CATG 7 cut(s) 64, 413, 431, 533, 574, 698, 1480
HspAI GCGC 2 cut(s) 178, 629
Kpn2I TCCGGA 1 cut(s) 1226
LmnI GCTCC 6 cut(s) 654, 760, 1038, 1168, 1344, 1586
LweI GCATC 5 cut(s) 250, 731, 979, 1388, 1584
MaeI CTAG 2 cut(s) 156, 249
MaeII ACGT 1 cut(s) 451
MaeIII GTNAC 1 cut(s) 423
MboII GAAGA 3 cut(s) 142, 148, 282
MflI RGATCY 6 cut(s) 55, 217, 227, 481, 1205, 1319
MhlI GDGCHC 2 cut(s) 451, 1444
MluCI AATT 8 cut(s) 79, 235, 358, 419, 490, 548, 794, 1210
MlyI GAGTC 2 cut(s) 311, 377
MmeI TCCRAC 5 cut(s) 193, 826, 947, 1060, 1193
MroI TCCGGA 1 cut(s) 1226
MseI TTAA 1 cut(s) 234
MslI CAYNNNNRTG 1 cut(s) 1308
MspA1I CMGCKG 2 cut(s) 463, 1395
MspI CCGG 7 cut(s) 195, 225, 278, 677, 1058, 1227, 1257
MspR9I CCNGG 4 cut(s) 1058, 1131, 1197, 1258
MvaI CCWGG 2 cut(s) 1131, 1197
MwoI GCNNNNNNNGC 8 cut(s) 293, 749, 1021, 1030, 1603, 1606, 1609, 1615
NciI CCSGG 2 cut(s) 1058, 1258
NlaIII CATG 7 cut(s) 64, 413, 431, 533, 574, 698, 1480
NlaIV GGNNCC 4 cut(s) 1065, 1327, 1484, 1493
NspI RCATGY 2 cut(s) 431, 698
PaeR7I CTCGAG 1 cut(s) 485
PagI TCATGA 1 cut(s) 60
PciI ACATGT 2 cut(s) 427, 694
PfeI GAWTC 3 cut(s) 131, 1241, 1418
PinAI ACCGGT 1 cut(s) 277
Ple19I CGATCG 2 cut(s) 390, 394
PleI GAGTC 2 cut(s) 311, 376
PpsI GAGTC 2 cut(s) 311, 376
PscI ACATGT 2 cut(s) 427, 694
Psp6I CCWGG 2 cut(s) 1129, 1195
PspFI CCCAGC 1 cut(s) 912
PspGI CCWGG 2 cut(s) 1129, 1195
PspN4I GGNNCC 4 cut(s) 1065, 1327, 1484, 1493
PspPI GGNCC 6 cut(s) 28, 406, 778, 1193, 1259, 1492
PstI CTGCAG 1 cut(s) 754
PstNI CAGNNNCTG 2 cut(s) 746, 1136
PsuI RGATCY 6 cut(s) 55, 217, 227, 481, 1205, 1319
PvuI CGATCG 2 cut(s) 390, 394
PvuII CAGCTG 1 cut(s) 463
RsaI GTAC 2 cut(s) 594, 1154
RsaNI GTAC 2 cut(s) 593, 1153
RseI CAYNNNNRTG 1 cut(s) 1308
SaqAI TTAA 1 cut(s) 234
Sau96I GGNCC 6 cut(s) 28, 406, 778, 1193, 1259, 1492
ScaI AGTACT 1 cut(s) 1154
SchI GAGTC 2 cut(s) 311, 377
ScrFI CCNGG 4 cut(s) 1058, 1131, 1197, 1258
SduI GDGCHC 2 cut(s) 451, 1444
SfaNI GCATC 5 cut(s) 250, 731, 979, 1388, 1584
SfcI CTRYAG 1 cut(s) 750
Sfr274I CTCGAG 1 cut(s) 485
SinI GGWCC 2 cut(s) 1193, 1492
SlaI CTCGAG 1 cut(s) 485
SmiMI CAYNNNNRTG 1 cut(s) 1308
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
Sse9I AATT 8 cut(s) 79, 235, 358, 419, 490, 548, 794, 1210
SspMI CTAG 2 cut(s) 156, 249
StyD4I CCNGG 4 cut(s) 1056, 1129, 1195, 1256
StyI CCWWGG 1 cut(s) 1487
TaaI ACNGT 9 cut(s) 110, 527, 590, 597, 824, 1152, 1272, 1510, 1532
TaiI ACGT 1 cut(s) 454
TaqI TCGA 7 cut(s) 12, 134, 324, 355, 390, 486, 1449
TasI AATT 8 cut(s) 79, 235, 358, 419, 490, 548, 794, 1210
TatI WGTACW 2 cut(s) 592, 1152
TauI GCSGC 3 cut(s) 718, 1024, 1203
TfiI GAWTC 3 cut(s) 131, 1241, 1418
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TscAI CASTG 3 cut(s) 451, 712, 1515
TspDTI ATGAA 4 cut(s) 77, 416, 828, 1586
TspGWI ACGGA 1 cut(s) 888
TspRI CASTG 3 cut(s) 451, 712, 1515
VneI GTGCAC 1 cut(s) 447
VpaK11BI GGWCC 2 cut(s) 1193, 1492
XapI RAATTY 2 cut(s) 358, 490
XceI RCATGY 2 cut(s) 431, 698
XhoI CTCGAG 1 cut(s) 485
XmiI GTMKAC 1 cut(s) 1253
XspI CTAG 2 cut(s) 156, 249
ZrmI AGTACT 1 cut(s) 1154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.