MD09G1028500.v1.1

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004149
Physical Location & Seq
Reverse (-)
1761702 .. 1764993
3292 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1028500.v1.1.491

Sequence Viewer

Length: 1752 bp
ATGAACACGACGTCGTTTCTGGACAAGCAGATAATGGATCTGTCGCAGGGATCGAAGCAGCAGCAGCACCAGAACGACGACTTCATCGACCTGATGAAGATGAACACCAACACCAACAACAGCCGCCGCAAAGACGGCGGAGGAGAAGAAGAGCATCAGGTCGGCGGTACCGGCAATGGAAATGGGATTTCCAAGAAGGAGTTTCAAGAGATGCTTCCCAGCTACGATTTCCAGCCGATTCGCCCCGTCGTCGGTGCTTCTTCCCCGTCGCGGAGTTTCGATGCTGCGCCCAATCTCGGAGGAGGAGGAGCGACTAGGGCTTGGAACTCTGGCGAGTCCAAGTCGAACACTGCATCTCCAATCCGAAATTATGGTTCAATGGATTCCCTTGAACCTGGAAAACTTATTTTAGAGAAGGACCGCAATGCTGCAATTGTGTCAGAGATTGACCAGACCATGAAGAAGCATGCCGATAATTTGCTACATATGTTGGAAGGCGTTAGTGCACGACTAACACAACTAGAGAGCAGAACCCGCAATCTTGAGAATTCTGTGGATGATTTGAAGATATCTGTTGGAAACAATCATGGGAATACTGATGGAACGATGCGACAGTTGGAGGGTATTCTTAGAGATGTGCAAACAGGTGTTAAGGATATGAAGGATAAGCAAGAAATAGTAGAGGCTCAACTGAATCTTGCAAGGATACAAATGTCCAATCCCAAGATAGACCCTCAACCAGAACCTCAGAATGCTGGACATGTGGATTCTGGGCAGACACCTGCTTCTGCTCCTCAACATTCTCACCAACAAATTCATCCCCCCGTGAACCTTCCTCCCTCAATTCCTGCTGTCTCTCATCCAAATGCTCCTTCCCAACCTATGCCCCAAAGTGTACCACATACAGTTCAGCATCCAAATCAGTTCTCTCAGAACCAGATCCCTACCGTTCCTCAGCGAGACCCTTATTTCCCAGCGCCTGGTCAAACTCAAGAAGCTCCAAATCAGCAATACCAATTACCTCCAGGCCAGCAGCCAGTTCCCCCTCCTCCAGTGCCACCACATCAACAATTTCAACCAGCCACTCAACCACGGTATTCCCAGCCACCGCCTCAGCTGCCTCAACAGCACCCTTCGCTTGCCCCTGTTAATCCCTCTCAGATCCAGCCTACACTAGGCCACCATGCTGAAGAGTCGCCCTATATTCCTTCCCAGAACTACCCACCTACTCTTCGGCAGCCACCTTCTCATACGCCCGGCAGTCTTCCCCCATCCCAACAGTATTACAGTCCAGCTGCAAATGCATATGAGCCACCACCTAGCAGACCCAGTTCAGGGTATCCTTCCGGGTATAACCCTCCATCTGGGCTTGGTGAATCGTACCAATACGGTGGACCACCTTCTCAATATGGCGGTAGCTCCTCAATGAAACCACAACATCACTCTTCTTCTGCTACAGCTCAAAGTGGAGGCAGTGGTTACCCACAGCTCCCAACTGCTCGGGTGCTACCACATGCCAATCCTACTCCATCTGGGCCGGGTGGCAGTTCACCTTCATCCGGTAGTGGAAGCAGAGTACCCATTGATGATGTGATTGACCAAGTGGCAACTATGGGGTTCTCCAGAGACCAAGTCAGGGCCGCAGTTCAGAAGCTGACAGACAGCGGCCAGGCAGTTGACCTCAATGTGGTGCTAGATAAGTTGATGAATGACGGGGATGTTCAGCAGCCCCGAGGTTGGTATGGGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
DUF1421

Protein Analysis

584

Amino Acids

62.85

Weight (kDa)

6.12

Isoelectric Point (pI)

59.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 528 - 571 2.2e-18 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 790
AatII GACGTC 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 790
Acc65I GGTACC 1 cut(s) 167
AccB1I GGYRCC 1 cut(s) 167
AccB7I CCANNNNNTGG 1 cut(s) 980
AccII CGCG 1 cut(s) 271
AclWI GGATC 4 cut(s) 45, 58, 934, 1156
AcoI YGGCCR 1 cut(s) 1666
AcsI RAATTY 2 cut(s) 547, 813
AcuI CTGAAG 1 cut(s) 1209
AcyI GRCGYC 1 cut(s) 11
AfaI GTAC 4 cut(s) 169, 897, 1382, 1578
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 5 cut(s) 206, 378, 392, 565, 1076
AjnI CCWGG 4 cut(s) 394, 979, 1024, 1668
AjuI GAANNNNNNNTTGG 2 cut(s) 856, 888
AluBI AGCT 8 cut(s) 222, 998, 1117, 1295, 1419, 1460, 1489, 1654
AluI AGCT 8 cut(s) 222, 998, 1117, 1295, 1419, 1460, 1489, 1654
Alw21I GWGCWC 1 cut(s) 508
Alw26I GTCTC 3 cut(s) 859, 954, 1620
Alw44I GTGCAC 1 cut(s) 504
AlwI GGATC 4 cut(s) 45, 58, 934, 1156
AlwNI CAGNNNCTG 2 cut(s) 980, 1654
Ama87I CYCGRG 2 cut(s) 1500, 1731
AoxI GGCC 5 cut(s) 1027, 1177, 1535, 1638, 1666
ApaLI GTGCAC 1 cut(s) 504
ApoI RAATTY 2 cut(s) 547, 813
Asp718I GGTACC 1 cut(s) 167
AspLEI GCGC 2 cut(s) 289, 979
AspS9I GGNCC 4 cut(s) 418, 1394, 1535, 1638
AsuC2I CCSGG 3 cut(s) 1257, 1348, 1539
AsuHPI GGTGA 3 cut(s) 797, 1385, 1542
AvaI CYCGRG 2 cut(s) 1500, 1731
AvaII GGWCC 2 cut(s) 418, 1394
BaeGI GKGCMC 1 cut(s) 508
BaeI ACNNNNGTAYC 2 cut(s) 698, 731
BanI GGYRCC 1 cut(s) 167
BbsI GAAGAC 1 cut(s) 1256
Bbv12I GWGCWC 1 cut(s) 508
BbvCI CCTCAGC 2 cut(s) 954, 1113
BccI CCATC 4 cut(s) 593, 1279, 1369, 1537
BceAI ACGGC 1 cut(s) 151
BciT130I CCWGG 4 cut(s) 396, 981, 1026, 1670
BciVI GTATCC 2 cut(s) 699, 1350
BcnI CCSGG 3 cut(s) 1257, 1348, 1539
BcoDI GTCTC 3 cut(s) 859, 954, 1620
BfaI CTAG 5 cut(s) 315, 521, 1175, 1320, 1694
BfmI CTRYAG 1 cut(s) 1455
BfoI RGCGCY 1 cut(s) 980
BfuAI ACCTGC 1 cut(s) 790
BfuI GTATCC 2 cut(s) 699, 1350
Bme1390I CCNGG 7 cut(s) 396, 981, 1026, 1257, 1348, 1539, 1670
Bme18I GGWCC 2 cut(s) 418, 1394
BmeT110I CYCGRG 2 cut(s) 1500, 1731
BmgT120I GGNCC 4 cut(s) 418, 1394, 1535, 1638
BmiI GGNNCC 1 cut(s) 169
BmrFI CCNGG 7 cut(s) 396, 981, 1026, 1257, 1348, 1539, 1670
BmrI ACTGGG 1 cut(s) 1323
BmsI GCATC 6 cut(s) 163, 201, 271, 362, 597, 922
BmuI ACTGGG 1 cut(s) 1323
BpiI GAAGAC 1 cut(s) 1256
BpmI CTGGAG 3 cut(s) 1008, 1035, 1606
Bpu10I CCTNAGC 2 cut(s) 954, 1113
BpuEI CTTGAG 2 cut(s) 563, 975
BpuMI CCSGG 3 cut(s) 1257, 1348, 1539
BsaHI GRCGYC 1 cut(s) 11
BsaI GGTCTC 2 cut(s) 954, 1620
BsaJI CCNNGG 2 cut(s) 1091, 1732
BsaWI WCCGGW 1 cut(s) 1559
BsaXI ACNNNNNCTCC 2 cut(s) 340, 370
Bse118I RCCGGY 1 cut(s) 170
Bse1I ACTGG 3 cut(s) 1037, 1052, 1329
Bse3DI GCAATG 2 cut(s) 181, 430
BseBI CCWGG 4 cut(s) 396, 981, 1026, 1670
BseDI CCNNGG 2 cut(s) 1091, 1732
BseGI GGATG 7 cut(s) 562, 817, 859, 913, 1271, 1556, 1723
BseMI GCAATG 2 cut(s) 181, 430
BseMII CTCAG 5 cut(s) 761, 944, 968, 1127, 1172
BseNI ACTGG 3 cut(s) 1037, 1052, 1329
BseRI GAGGAG 7 cut(s) 156, 315, 318, 321, 783, 1038, 1411
BseSI GKGCMC 1 cut(s) 508
BseYI CCCAGC 3 cut(s) 218, 973, 1101
Bsh1236I CGCG 1 cut(s) 271
BshFI GGCC 5 cut(s) 1029, 1179, 1537, 1640, 1668
BshNI GGYRCC 1 cut(s) 167
BsiHKAI GWGCWC 1 cut(s) 508
BsiHKCI CYCGRG 2 cut(s) 1500, 1731
BsiSI CCGG 5 cut(s) 171, 1257, 1347, 1538, 1560
BsmAI GTCTC 3 cut(s) 859, 954, 1620
BsmI GAATGC 1 cut(s) 757
BsnI GGCC 5 cut(s) 1029, 1179, 1537, 1640, 1668
Bso31I GGTCTC 2 cut(s) 954, 1620
BsoBI CYCGRG 2 cut(s) 1500, 1731
Bsp1286I GDGCHC 1 cut(s) 508
Bsp143I GATC 4 cut(s) 37, 50, 939, 1161
BspANI GGCC 5 cut(s) 1029, 1179, 1537, 1640, 1668
BspCNI CTCAG 5 cut(s) 760, 943, 967, 1126, 1171
BspFNI CGCG 1 cut(s) 271
BspLI GGNNCC 1 cut(s) 169
BspMI ACCTGC 1 cut(s) 790
BspPI GGATC 4 cut(s) 45, 58, 934, 1156
BspQI GCTCTTC 1 cut(s) 144
BspT107I GGYRCC 1 cut(s) 167
BspTNI GGTCTC 2 cut(s) 954, 1620
BsrDI GCAATG 2 cut(s) 181, 430
BsrFI RCCGGY 1 cut(s) 170
BsrI ACTGG 3 cut(s) 1037, 1052, 1329
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 2 cut(s) 1091, 1732
BssMI GATC 4 cut(s) 37, 50, 939, 1161
BssNI GRCGYC 1 cut(s) 11
Bst2UI CCWGG 4 cut(s) 396, 981, 1026, 1670
Bst4CI ACNGT 7 cut(s) 615, 907, 949, 1095, 1281, 1289, 1391
Bst6I CTCTTC 4 cut(s) 144, 1185, 1236, 1450
BstACI GRCGYC 1 cut(s) 11
BstC8I GCNNGC 3 cut(s) 468, 1031, 1140
BstDEI CTNAG 6 cut(s) 629, 747, 930, 954, 1113, 1158
BstDSI CCRYGG 1 cut(s) 1091
BstEII GGTNACC 1 cut(s) 1478
BstENI CCTNNNNNAGG 1 cut(s) 1173
BstF5I GGATG 7 cut(s) 562, 817, 859, 913, 1271, 1556, 1723
BstFNI CGCG 1 cut(s) 271
BstH2I RGCGCY 1 cut(s) 980
BstHHI GCGC 2 cut(s) 289, 979
BstKTI GATC 4 cut(s) 40, 53, 942, 1164
BstMAI GTCTC 3 cut(s) 859, 954, 1620
BstMBI GATC 4 cut(s) 37, 50, 939, 1161
BstMWI GCNNNNNNNGC 9 cut(s) 64, 135, 171, 317, 534, 1117, 1126, 1135, 1301
BstNI CCWGG 4 cut(s) 396, 981, 1026, 1670
BstNSI RCATGY 3 cut(s) 470, 764, 1517
BstPI GGTNACC 1 cut(s) 1478
BstSCI CCNGG 7 cut(s) 394, 979, 1024, 1255, 1346, 1537, 1668
BstSFI CTRYAG 1 cut(s) 1455
BstSLI GKGCMC 1 cut(s) 508
BstUI CGCG 1 cut(s) 271
BstV2I GAAGAC 1 cut(s) 1256
BstX2I RGATCY 3 cut(s) 37, 939, 1161
BstXI CCANNNNNNTGG 1 cut(s) 1391
BstYI RGATCY 3 cut(s) 37, 939, 1161
BsuI GTATCC 2 cut(s) 699, 1350
BsuRI GGCC 5 cut(s) 1029, 1179, 1537, 1640, 1668
BtgI CCRYGG 1 cut(s) 1091
BtsCI GGATG 7 cut(s) 562, 817, 859, 913, 1271, 1556, 1723
BtsI GCAGTG 2 cut(s) 348, 1480
BtsIMutI CAGTG 3 cut(s) 348, 1059, 1480
BveI ACCTGC 1 cut(s) 790
Cac8I GCNNGC 3 cut(s) 468, 1031, 1140
CaiI CAGNNNCTG 2 cut(s) 980, 1654
CfoI GCGC 2 cut(s) 289, 979
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 4 cut(s) 418, 1394, 1535, 1638
Csp6I GTAC 4 cut(s) 168, 896, 1381, 1577
CviAII CATG 6 cut(s) 457, 467, 587, 761, 1184, 1514
CviQI GTAC 4 cut(s) 168, 896, 1381, 1577
DdeI CTNAG 6 cut(s) 629, 747, 930, 954, 1113, 1158
DpnI GATC 4 cut(s) 39, 52, 941, 1163
DpnII GATC 4 cut(s) 37, 50, 939, 1161
EaeI YGGCCR 1 cut(s) 1666
Eam1104I CTCTTC 4 cut(s) 144, 1185, 1236, 1450
EarI CTCTTC 4 cut(s) 144, 1185, 1236, 1450
EciI GGCGGA 1 cut(s) 153
Eco31I GGTCTC 2 cut(s) 954, 1620
Eco32I GATATC 1 cut(s) 570
Eco47I GGWCC 2 cut(s) 418, 1394
Eco57I CTGAAG 1 cut(s) 1209
Eco88I CYCGRG 2 cut(s) 1500, 1731
Eco91I GGTNACC 1 cut(s) 1478
EcoNI CCTNNNNNAGG 1 cut(s) 1173
EcoO65I GGTNACC 1 cut(s) 1478
EcoRI GAATTC 1 cut(s) 547
EcoRII CCWGG 4 cut(s) 394, 979, 1024, 1668
EcoRV GATATC 1 cut(s) 570
EcoT22I ATGCAT 1 cut(s) 1306
FaeI CATG 6 cut(s) 460, 470, 590, 764, 1187, 1517
FalI AAGNNNNNCTT 2 cut(s) 198, 230
FatI CATG 6 cut(s) 456, 466, 586, 760, 1183, 1513
FauI CCCGC 1 cut(s) 542
FauNDI CATATG 2 cut(s) 486, 1306
FokI GGATG 7 cut(s) 569, 804, 846, 900, 1258, 1543, 1730
FspBI CTAG 5 cut(s) 315, 521, 1175, 1320, 1694
GlaI GCGC 2 cut(s) 288, 978
GsaI CCCAGC 3 cut(s) 222, 977, 1105
GsuI CTGGAG 3 cut(s) 1008, 1035, 1606
HaeII RGCGCY 1 cut(s) 980
HaeIII GGCC 5 cut(s) 1029, 1179, 1537, 1640, 1668
HapII CCGG 5 cut(s) 171, 1257, 1347, 1538, 1560
HhaI GCGC 2 cut(s) 289, 979
Hin1I GRCGYC 1 cut(s) 11
Hin1II CATG 6 cut(s) 460, 470, 590, 764, 1187, 1517
Hin6I GCGC 2 cut(s) 287, 977
HinP1I GCGC 2 cut(s) 287, 977
HincII GTYRAC 1 cut(s) 1678
HindII GTYRAC 1 cut(s) 1678
HinfI GANTC 7 cut(s) 238, 335, 383, 694, 767, 1193, 1376
HpaII CCGG 5 cut(s) 171, 1257, 1347, 1538, 1560
HphI GGTGA 3 cut(s) 797, 1385, 1542
Hpy166II GTNNAC 6 cut(s) 506, 829, 896, 1394, 1550, 1678
Hpy188I TCNGA 7 cut(s) 299, 365, 442, 750, 933, 1161, 1650
Hpy188III TCNNGA 5 cut(s) 20, 206, 542, 992, 1623
Hpy8I GTNNAC 6 cut(s) 506, 829, 896, 1394, 1550, 1678
Hpy99I CGWCG 6 cut(s) 13, 16, 80, 251, 254, 271
HpyCH4III ACNGT 7 cut(s) 615, 907, 949, 1095, 1281, 1289, 1391
HpyCH4IV ACGT 1 cut(s) 11
HpyCH4V TGCA 7 cut(s) 353, 431, 506, 640, 701, 1298, 1304
HpyF10VI GCNNNNNNNGC 9 cut(s) 64, 135, 171, 317, 534, 1117, 1126, 1135, 1301
HpyF3I CTNAG 6 cut(s) 629, 747, 930, 954, 1113, 1158
HpySE526I ACGT 1 cut(s) 11
Hsp92I GRCGYC 1 cut(s) 11
Hsp92II CATG 6 cut(s) 460, 470, 590, 764, 1187, 1517
HspAI GCGC 2 cut(s) 287, 977
KpnI GGTACC 1 cut(s) 171
Kzo9I GATC 4 cut(s) 37, 50, 939, 1161
LguI GCTCTTC 1 cut(s) 144
LmnI GCTCC 6 cut(s) 308, 796, 874, 1003, 1424, 1494
LweI GCATC 6 cut(s) 163, 201, 271, 362, 597, 922
MaeI CTAG 5 cut(s) 315, 521, 1175, 1320, 1694
MaeII ACGT 1 cut(s) 11
MaeIII GTNAC 1 cut(s) 1478
MalI GATC 4 cut(s) 39, 52, 941, 1163
MboI GATC 4 cut(s) 37, 50, 939, 1161
MfeI CAATTG 1 cut(s) 432
MflI RGATCY 3 cut(s) 37, 939, 1161
MhlI GDGCHC 1 cut(s) 508
MluCI AATT 8 cut(s) 367, 432, 475, 547, 813, 843, 1016, 1070
MlyI GAGTC 2 cut(s) 344, 1202
MmeI TCCRAC 3 cut(s) 471, 556, 597
Mph1103I ATGCAT 1 cut(s) 1306
MseI TTAA 2 cut(s) 651, 1149
MslI CAYNNNNRTG 1 cut(s) 864
MspA1I CMGCKG 3 cut(s) 1117, 1295, 1665
MspI CCGG 5 cut(s) 171, 1257, 1347, 1538, 1560
MspR9I CCNGG 7 cut(s) 396, 981, 1026, 1257, 1348, 1539, 1670
MunI CAATTG 1 cut(s) 432
Mva1269I GAATGC 1 cut(s) 757
MvaI CCWGG 4 cut(s) 396, 981, 1026, 1670
MvnI CGCG 1 cut(s) 271
MwoI GCNNNNNNNGC 9 cut(s) 64, 135, 171, 317, 534, 1117, 1126, 1135, 1301
NciI CCSGG 3 cut(s) 1257, 1348, 1539
NdeI CATATG 2 cut(s) 486, 1306
NdeII GATC 4 cut(s) 37, 50, 939, 1161
NlaIII CATG 6 cut(s) 460, 470, 590, 764, 1187, 1517
NlaIV GGNNCC 1 cut(s) 169
NsiI ATGCAT 1 cut(s) 1306
NspI RCATGY 3 cut(s) 470, 764, 1517
PaeI GCATGC 1 cut(s) 470
PaqCI CACCTGC 1 cut(s) 790
PciI ACATGT 1 cut(s) 760
PciSI GCTCTTC 1 cut(s) 144
PcsI WCGNNNNNNNCGW 2 cut(s) 50, 84
PctI GAATGC 1 cut(s) 757
PfeI GAWTC 5 cut(s) 238, 383, 694, 767, 1376
PflFI GACNNNGTC 1 cut(s) 1631
PflMI CCANNNNNTGG 1 cut(s) 980
PleI GAGTC 2 cut(s) 343, 1201
PpsI GAGTC 2 cut(s) 343, 1201
PscI ACATGT 1 cut(s) 760
Psp6I CCWGG 4 cut(s) 394, 979, 1024, 1668
PspEI GGTNACC 1 cut(s) 1478
PspFI CCCAGC 3 cut(s) 218, 973, 1101
PspGI CCWGG 4 cut(s) 394, 979, 1024, 1668
PspN4I GGNNCC 1 cut(s) 169
PspPI GGNCC 4 cut(s) 418, 1394, 1535, 1638
PstNI CAGNNNCTG 2 cut(s) 980, 1654
PsuI RGATCY 3 cut(s) 37, 939, 1161
PsyI GACNNNGTC 1 cut(s) 1631
PvuII CAGCTG 2 cut(s) 1117, 1295
RsaI GTAC 4 cut(s) 169, 897, 1382, 1578
RsaNI GTAC 4 cut(s) 168, 896, 1381, 1577
RseI CAYNNNNRTG 1 cut(s) 864
SapI GCTCTTC 1 cut(s) 144
SaqAI TTAA 2 cut(s) 651, 1149
Sau3AI GATC 4 cut(s) 37, 50, 939, 1161
Sau96I GGNCC 4 cut(s) 418, 1394, 1535, 1638
SchI GAGTC 2 cut(s) 344, 1202
ScrFI CCNGG 7 cut(s) 396, 981, 1026, 1257, 1348, 1539, 1670
SduI GDGCHC 1 cut(s) 508
SfaNI GCATC 6 cut(s) 163, 201, 271, 362, 597, 922
SfcI CTRYAG 1 cut(s) 1455
SinI GGWCC 2 cut(s) 418, 1394
SmiMI CAYNNNNRTG 1 cut(s) 864
SmlI CTYRAG 2 cut(s) 542, 990
SmoI CTYRAG 2 cut(s) 542, 990
SphI GCATGC 1 cut(s) 470
Sse9I AATT 8 cut(s) 367, 432, 475, 547, 813, 843, 1016, 1070
SspMI CTAG 5 cut(s) 315, 521, 1175, 1320, 1694
StyD4I CCNGG 7 cut(s) 394, 979, 1024, 1255, 1346, 1537, 1668
TaaI ACNGT 7 cut(s) 615, 907, 949, 1095, 1281, 1289, 1391
TaiI ACGT 1 cut(s) 14
TaqI TCGA 4 cut(s) 53, 87, 279, 344
TasI AATT 8 cut(s) 367, 432, 475, 547, 813, 843, 1016, 1070
TauI GCSGC 4 cut(s) 126, 129, 1643, 1668
TfiI GAWTC 5 cut(s) 238, 383, 694, 767, 1376
Tru1I TTAA 2 cut(s) 651, 1149
Tru9I TTAA 2 cut(s) 651, 1149
TscAI CASTG 3 cut(s) 355, 1059, 1480
TspRI CASTG 3 cut(s) 355, 1059, 1480
Tth111I GACNNNGTC 1 cut(s) 1631
Van91I CCANNNNNTGG 1 cut(s) 980
VneI GTGCAC 1 cut(s) 504
VpaK11BI GGWCC 2 cut(s) 418, 1394
XagI CCTNNNNNAGG 1 cut(s) 1173
XapI RAATTY 2 cut(s) 547, 813
XceI RCATGY 3 cut(s) 470, 764, 1517
XspI CTAG 5 cut(s) 315, 521, 1175, 1320, 1694
ZraI GACGTC 1 cut(s) 12
Zsp2I ATGCAT 1 cut(s) 1306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.