MD17G1030000.v1.1

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004149
Physical Location & Seq
Reverse (-)
2160848 .. 2164109
3262 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1030000.v1.1.491

Sequence Viewer

Length: 1659 bp
ATGGATCTGTCGCAGGGATCAGCGCAGCAGCAGCACCACCAGAACGACGACTTCATCAACCTGATGAAGATTGACAGCAACACCAGCAACAGCCACCTCGAAGGCGGAGGAGAAGAAGAGTATCAGGTCGGCGGGGGCGGCCATGGAAGTGGGATTTACAACAAGGAGAATCAGGATATGCTTCCCAGCTACGATTTCCAGCCGATTCGCCCCGTCGTCGGTGCTTCTTCTCCGTCGCCGAGTTTCGATGCCGCGCCTAATCTTGGAGGAGGAGGACCGACCAGGGCGTGGAACTCCGGCGAGTCCAAGTCGAACGCTGCATCTCCAATCAGAAATTATGGGTCAATGGATTCCCTTGAACCTGCAAAACTTGTTTTAGAGAAGGACCGCAATGTCGTAATTCTGTCAGAGATTGACCAGACCATGAAGAAGCATGCTGATAATTTGCTACATGTGCTGGAAGGTGTTAGTGCACGACTAACACAACTAGAGAGCAGAAGCCGCAATCTTGAGAATTCTGTCGATGATTTGAAGGGTATACTTAGAGATGTGCAAACAGATGTTAAGGATTTGAAGGATGCGCAAGAAATGGTAGAACTGAATCTTGCAAGGATACAAGTATCGAATACAAAGATAGACCCTCAACTGGAATCTCAGAATGCTGGACAACGACATACTGAGCAGACAGCCGCCTCTGCTCCTCAACATTCTCATCAACAAATTCATCCCCCCATGAACCTTCCTCCCTCACTTCCTGGTGTTTCTTATCCAAATGCTCCTCCCCAACCTATCCCCCAAAATGCACCACATGCGGTTCAACTTCCAAATCAGTTTTCTCAGAACCAGATCCCTCCTGTCCCTCAGAGAGACCCTTATTTCCCAGCACCTGGTCAAACTCAAGAAGCCCCAAATCATCAATACCAGTTACCTCCAGGTCAGCAGCCTGCCCCCCTTCCTCCAGTGCCACCGCATCAACAATTTCAACCTACCACTCAACCACAGTATTCCCAGCCGCCACCCCAGCTGCCTCAACAGCACCCTTCGCTTGCACCTGTTAATCCCTCTCAAATCCAGCCTAAATTAGGCCACCACACTGAAGAGTCACCTTATATCCCTTCCCAGAACTACCCACCAAGTCTTCGCCAGCCACCTTCTCATACACCCAGCGGTCACCCCCCTTCCCAACAGTATTACAGTCCAGCTTCAAATGCATATGAGCCACCTTCTAGCAGACCCAGTGCAGGGTATCCTTCCGGGTATAACCCTCCATCTGGGCTTGGTGAATCGTACCAATACGGTGGACCACCTTCTCAATATGGCGGTAGCTCCACAATGCAACCACAACATCACTCTTCTTCTGCTACAGCTCAAAGTGGAGGCAGTGGTTACCCACAGCTCCCAACTGCTCGGGTGCTACCACATGCCAACCCTACTCCATCTGGGGCGGGTGGCAGTCCACCTTCATCCGGTAGTGGAAACAGAGTACCCATTGACGATGTGATTGACCAAGTGGCAACTATGGGTTTCTCCAGAGACCAAGTTAGGGCTGCAGTTCGGAAGCTGACAGACAGCGGCCAGTCAGTTGACCTCAATGTGGTGCTAGATAAGATGATGACTGACAGCGATGTTCAGCCACCCCGAGGATGGTATGGCCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
DUF1421

Protein Analysis

553

Amino Acids

59.46

Weight (kDa)

5.73

Isoelectric Point (pI)

63.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 497 - 541 2e-16 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 392
Acc16I TGCGCA 1 cut(s) 582
Acc36I ACCTGC 1 cut(s) 370
AccB7I CCANNNNNTGG 2 cut(s) 288, 887
AccI GTMKAC 1 cut(s) 538
AccII CGCG 1 cut(s) 254
AclWI GGATC 3 cut(s) 12, 25, 841
AcoI YGGCCR 3 cut(s) 139, 1573, 1651
AcsI RAATTY 2 cut(s) 514, 720
AcuI CTGAAG 1 cut(s) 1116
AfaI GTAC 2 cut(s) 1289, 1485
AflIII ACRYGT 1 cut(s) 451
AgsI TTSAA 6 cut(s) 359, 532, 574, 818, 983, 1206
AjnI CCWGG 4 cut(s) 281, 754, 886, 931
AjuI GAANNNNNNNTTGG 2 cut(s) 817, 849
AluBI AGCT 7 cut(s) 189, 1024, 1202, 1326, 1367, 1396, 1561
AluI AGCT 7 cut(s) 189, 1024, 1202, 1326, 1367, 1396, 1561
Alw21I GWGCWC 1 cut(s) 475
Alw26I GTCTC 2 cut(s) 861, 1527
Alw44I GTGCAC 1 cut(s) 471
AlwI GGATC 3 cut(s) 12, 25, 841
AlwNI CAGNNNCTG 1 cut(s) 887
Ama87I CYCGRG 2 cut(s) 1407, 1638
AoxI GGCC 4 cut(s) 139, 1084, 1573, 1651
ApaLI GTGCAC 1 cut(s) 471
ApeKI GCWGC 7 cut(s) 25, 28, 31, 317, 940, 1024, 1547
ApoI RAATTY 2 cut(s) 514, 720
AspLEI GCGC 3 cut(s) 25, 256, 583
AspS9I GGNCC 3 cut(s) 275, 385, 1301
AsuC2I CCSGG 1 cut(s) 1255
AsuHPI GGTGA 3 cut(s) 1095, 1163, 1292
AvaI CYCGRG 2 cut(s) 1407, 1638
AvaII GGWCC 3 cut(s) 275, 385, 1301
BaeGI GKGCMC 1 cut(s) 475
BbsI GAAGAC 1 cut(s) 1130
Bbv12I GWGCWC 1 cut(s) 475
BbvI GCAGC 7 cut(s) 37, 40, 43, 304, 952, 1011, 1534
BccI CCATC 3 cut(s) 1276, 1444, 1638
BciT130I CCWGG 4 cut(s) 283, 756, 888, 933
BciVI GTATCC 2 cut(s) 606, 1257
BcnI CCSGG 1 cut(s) 1255
BcoDI GTCTC 2 cut(s) 861, 1527
BfaI CTAG 3 cut(s) 488, 1227, 1601
BfmI CTRYAG 2 cut(s) 1362, 1548
BfuAI ACCTGC 1 cut(s) 370
BfuI GTATCC 2 cut(s) 606, 1257
Bme1390I CCNGG 5 cut(s) 283, 756, 888, 933, 1255
Bme18I GGWCC 3 cut(s) 275, 385, 1301
BmeT110I CYCGRG 2 cut(s) 1407, 1638
BmgT120I GGNCC 3 cut(s) 275, 385, 1301
BmrFI CCNGG 5 cut(s) 283, 756, 888, 933, 1255
BmrI ACTGGG 1 cut(s) 1230
BmsI GCATC 4 cut(s) 238, 329, 568, 979
BmuI ACTGGG 1 cut(s) 1230
BpiI GAAGAC 1 cut(s) 1130
BpmI CTGGAG 3 cut(s) 915, 942, 1513
BpuEI CTTGAG 2 cut(s) 530, 882
BpuMI CCSGG 1 cut(s) 1255
BsaI GGTCTC 2 cut(s) 861, 1527
BsaJI CCNNGG 3 cut(s) 142, 282, 1639
BsaWI WCCGGW 1 cut(s) 1466
Bse1I ACTGG 5 cut(s) 651, 922, 959, 1236, 1576
Bse3DI GCAATG 1 cut(s) 397
BseBI CCWGG 4 cut(s) 283, 756, 888, 933
BseDI CCNNGG 3 cut(s) 142, 282, 1639
BseGI GGATG 4 cut(s) 583, 724, 1463, 1649
BseMI GCAATG 1 cut(s) 397
BseMII CTCAG 4 cut(s) 668, 669, 851, 875
BseNI ACTGG 5 cut(s) 651, 922, 959, 1236, 1576
BseRI GAGGAG 5 cut(s) 123, 282, 285, 690, 768
BseSI GKGCMC 1 cut(s) 475
BseXI GCAGC 7 cut(s) 37, 40, 43, 304, 952, 1011, 1534
BseYI CCCAGC 5 cut(s) 185, 880, 1008, 1020, 1163
BsgI GTGCAG 1 cut(s) 1260
Bsh1236I CGCG 1 cut(s) 254
BshFI GGCC 4 cut(s) 141, 1086, 1575, 1653
BsiHKAI GWGCWC 1 cut(s) 475
BsiHKCI CYCGRG 2 cut(s) 1407, 1638
BsiSI CCGG 3 cut(s) 297, 1254, 1467
BslFI GGGAC 1 cut(s) 842
BsmAI GTCTC 2 cut(s) 861, 1527
BsmFI GGGAC 1 cut(s) 842
BsmI GAATGC 1 cut(s) 664
BsnI GGCC 4 cut(s) 141, 1086, 1575, 1653
Bso31I GGTCTC 2 cut(s) 861, 1527
BsoBI CYCGRG 2 cut(s) 1407, 1638
Bsp1286I GDGCHC 1 cut(s) 475
Bsp143I GATC 3 cut(s) 4, 17, 846
Bsp19I CCATGG 1 cut(s) 142
BspANI GGCC 4 cut(s) 141, 1086, 1575, 1653
BspCNI CTCAG 4 cut(s) 667, 670, 850, 874
BspFNI CGCG 1 cut(s) 254
BspMAI CTGCAG 1 cut(s) 1552
BspMI ACCTGC 1 cut(s) 370
BspPI GGATC 3 cut(s) 12, 25, 841
BspTNI GGTCTC 2 cut(s) 861, 1527
BsrDI GCAATG 1 cut(s) 397
BsrI ACTGG 5 cut(s) 651, 922, 959, 1236, 1576
BssECI CCNNGG 3 cut(s) 142, 282, 1639
BssMI GATC 3 cut(s) 4, 17, 846
BssNAI GTATAC 1 cut(s) 539
BssT1I CCWWGG 1 cut(s) 142
Bst1107I GTATAC 1 cut(s) 539
Bst2UI CCWGG 4 cut(s) 283, 756, 888, 933
Bst4CI ACNGT 4 cut(s) 1002, 1188, 1196, 1298
Bst6I CTCTTC 3 cut(s) 111, 1092, 1357
BstAPI GCANNNNNTGC 1 cut(s) 809
BstC8I GCNNGC 4 cut(s) 435, 945, 1047, 1145
BstDEI CTNAG 5 cut(s) 542, 654, 678, 837, 861
BstDSI CCRYGG 1 cut(s) 142
BstEII GGTNACC 2 cut(s) 1169, 1385
BstENI CCTNNNNNAGG 1 cut(s) 1080
BstF5I GGATG 4 cut(s) 583, 724, 1463, 1649
BstFNI CGCG 1 cut(s) 254
BstHHI GCGC 3 cut(s) 25, 256, 583
BstKTI GATC 3 cut(s) 7, 20, 849
BstMAI GTCTC 2 cut(s) 861, 1527
BstMBI GATC 3 cut(s) 4, 17, 846
BstNI CCWGG 4 cut(s) 283, 756, 888, 933
BstNSI RCATGY 4 cut(s) 437, 455, 812, 1424
BstPI GGTNACC 2 cut(s) 1169, 1385
BstSCI CCNGG 5 cut(s) 281, 754, 886, 931, 1253
BstSFI CTRYAG 2 cut(s) 1362, 1548
BstSLI GKGCMC 1 cut(s) 475
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 7 cut(s) 37, 40, 43, 304, 952, 1011, 1534
BstV2I GAAGAC 1 cut(s) 1130
BstX2I RGATCY 2 cut(s) 4, 846
BstXI CCANNNNNNTGG 2 cut(s) 149, 1298
BstYI RGATCY 2 cut(s) 4, 846
BstZ17I GTATAC 1 cut(s) 539
BsuI GTATCC 2 cut(s) 606, 1257
BsuRI GGCC 4 cut(s) 141, 1086, 1575, 1653
BtgI CCRYGG 1 cut(s) 142
BtgZI GCGATG 1 cut(s) 1638
BtsCI GGATG 4 cut(s) 583, 724, 1463, 1649
BtsI GCAGTG 1 cut(s) 1387
BtsIMutI CAGTG 4 cut(s) 966, 1092, 1243, 1387
BveI ACCTGC 1 cut(s) 370
Cac8I GCNNGC 4 cut(s) 435, 945, 1047, 1145
CaiI CAGNNNCTG 1 cut(s) 887
CfoI GCGC 3 cut(s) 25, 256, 583
Cfr13I GGNCC 3 cut(s) 275, 385, 1301
CsiI ACCWGGT 1 cut(s) 886
Csp6I GTAC 2 cut(s) 1288, 1484
CviAII CATG 7 cut(s) 143, 424, 434, 452, 733, 809, 1421
CviQI GTAC 2 cut(s) 1288, 1484
DdeI CTNAG 5 cut(s) 542, 654, 678, 837, 861
DpnI GATC 3 cut(s) 6, 19, 848
DpnII GATC 3 cut(s) 4, 17, 846
DrdI GACNNNNNNGTC 1 cut(s) 392
DseDI GACNNNNNNGTC 1 cut(s) 392
EaeI YGGCCR 3 cut(s) 139, 1573, 1651
Eam1104I CTCTTC 3 cut(s) 111, 1092, 1357
EarI CTCTTC 3 cut(s) 111, 1092, 1357
EciI GGCGGA 1 cut(s) 120
Eco130I CCWWGG 1 cut(s) 142
Eco31I GGTCTC 2 cut(s) 861, 1527
Eco47I GGWCC 3 cut(s) 275, 385, 1301
Eco57I CTGAAG 1 cut(s) 1116
Eco88I CYCGRG 2 cut(s) 1407, 1638
Eco91I GGTNACC 2 cut(s) 1169, 1385
EcoNI CCTNNNNNAGG 1 cut(s) 1080
EcoO65I GGTNACC 2 cut(s) 1169, 1385
EcoRI GAATTC 1 cut(s) 514
EcoRII CCWGG 4 cut(s) 281, 754, 886, 931
EcoT14I CCWWGG 1 cut(s) 142
EcoT22I ATGCAT 1 cut(s) 1213
ErhI CCWWGG 1 cut(s) 142
FaeI CATG 7 cut(s) 146, 427, 437, 455, 736, 812, 1424
FaqI GGGAC 1 cut(s) 842
FatI CATG 7 cut(s) 142, 423, 433, 451, 732, 808, 1420
FauI CCCGC 2 cut(s) 125, 1438
FauNDI CATATG 1 cut(s) 1213
FblI GTMKAC 1 cut(s) 538
FokI GGATG 3 cut(s) 590, 711, 1450
FspBI CTAG 3 cut(s) 488, 1227, 1601
FspI TGCGCA 1 cut(s) 582
GlaI GCGC 3 cut(s) 24, 255, 582
GsaI CCCAGC 5 cut(s) 189, 884, 1012, 1024, 1167
GsuI CTGGAG 3 cut(s) 915, 942, 1513
HaeIII GGCC 4 cut(s) 141, 1086, 1575, 1653
HapII CCGG 3 cut(s) 297, 1254, 1467
HhaI GCGC 3 cut(s) 25, 256, 583
Hin1II CATG 7 cut(s) 146, 427, 437, 455, 736, 812, 1424
Hin6I GCGC 3 cut(s) 23, 254, 581
HinP1I GCGC 3 cut(s) 23, 254, 581
HincII GTYRAC 1 cut(s) 1585
HindII GTYRAC 1 cut(s) 1585
HinfI GANTC 8 cut(s) 169, 205, 302, 350, 601, 650, 1100, 1283
HpaII CCGG 3 cut(s) 297, 1254, 1467
HphI GGTGA 3 cut(s) 1095, 1163, 1292
Hpy166II GTNNAC 5 cut(s) 473, 539, 1301, 1457, 1585
Hpy188I TCNGA 6 cut(s) 332, 409, 657, 840, 864, 1557
Hpy188III TCNNGA 4 cut(s) 173, 509, 899, 1530
Hpy8I GTNNAC 5 cut(s) 473, 539, 1301, 1457, 1585
Hpy99I CGWCG 4 cut(s) 50, 218, 221, 238
HpyCH4III ACNGT 4 cut(s) 1002, 1188, 1196, 1298
HpyF3I CTNAG 5 cut(s) 542, 654, 678, 837, 861
Hsp92II CATG 7 cut(s) 146, 427, 437, 455, 736, 812, 1424
HspAI GCGC 3 cut(s) 23, 254, 581
Kzo9I GATC 3 cut(s) 4, 17, 846
LmnI GCTCC 4 cut(s) 703, 781, 1331, 1401
Lsp1109I GCAGC 7 cut(s) 37, 40, 43, 304, 952, 1011, 1534
LweI GCATC 4 cut(s) 238, 329, 568, 979
MabI ACCWGGT 1 cut(s) 886
MaeI CTAG 3 cut(s) 488, 1227, 1601
MaeIII GTNAC 4 cut(s) 924, 1101, 1169, 1385
MalI GATC 3 cut(s) 6, 19, 848
MboI GATC 3 cut(s) 4, 17, 846
MboII GAAGA 9 cut(s) 79, 125, 128, 219, 439, 1109, 1130, 1344, 1347
MflI RGATCY 2 cut(s) 4, 846
MhlI GDGCHC 1 cut(s) 475
MluCI AATT 7 cut(s) 334, 399, 442, 514, 720, 977, 1079
MlyI GAGTC 2 cut(s) 311, 1109
Mph1103I ATGCAT 1 cut(s) 1213
MseI TTAA 2 cut(s) 564, 1056
MslI CAYNNNNRTG 1 cut(s) 147
MspA1I CMGCKG 3 cut(s) 1024, 1167, 1572
MspI CCGG 3 cut(s) 297, 1254, 1467
MspR9I CCNGG 5 cut(s) 283, 756, 888, 933, 1255
Mva1269I GAATGC 1 cut(s) 664
MvaI CCWGG 4 cut(s) 283, 756, 888, 933
MvnI CGCG 1 cut(s) 254
NciI CCSGG 1 cut(s) 1255
NcoI CCATGG 1 cut(s) 142
NdeI CATATG 1 cut(s) 1213
NdeII GATC 3 cut(s) 4, 17, 846
NlaIII CATG 7 cut(s) 146, 427, 437, 455, 736, 812, 1424
NmeAIII GCCGAG 1 cut(s) 264
NmuCI GTSAC 2 cut(s) 1101, 1169
NsbI TGCGCA 1 cut(s) 582
NsiI ATGCAT 1 cut(s) 1213
NspI RCATGY 4 cut(s) 437, 455, 812, 1424
PaeI GCATGC 1 cut(s) 437
PciI ACATGT 1 cut(s) 451
PctI GAATGC 1 cut(s) 664
PfeI GAWTC 6 cut(s) 169, 205, 350, 601, 650, 1283
PflMI CCANNNNNTGG 2 cut(s) 288, 887
PleI GAGTC 2 cut(s) 310, 1108
PpsI GAGTC 2 cut(s) 310, 1108
PscI ACATGT 1 cut(s) 451
Psp6I CCWGG 4 cut(s) 281, 754, 886, 931
PspEI GGTNACC 2 cut(s) 1169, 1385
PspFI CCCAGC 5 cut(s) 185, 880, 1008, 1020, 1163
PspGI CCWGG 4 cut(s) 281, 754, 886, 931
PspPI GGNCC 3 cut(s) 275, 385, 1301
PstI CTGCAG 1 cut(s) 1552
PstNI CAGNNNCTG 1 cut(s) 887
PsuI RGATCY 2 cut(s) 4, 846
PvuII CAGCTG 1 cut(s) 1024
RsaI GTAC 2 cut(s) 1289, 1485
RsaNI GTAC 2 cut(s) 1288, 1484
RseI CAYNNNNRTG 1 cut(s) 147
SaqAI TTAA 2 cut(s) 564, 1056
Sau3AI GATC 3 cut(s) 4, 17, 846
Sau96I GGNCC 3 cut(s) 275, 385, 1301
SchI GAGTC 2 cut(s) 311, 1109
ScrFI CCNGG 5 cut(s) 283, 756, 888, 933, 1255
SduI GDGCHC 1 cut(s) 475
SexAI ACCWGGT 1 cut(s) 886
SfaNI GCATC 4 cut(s) 238, 329, 568, 979
SfcI CTRYAG 2 cut(s) 1362, 1548
SinI GGWCC 3 cut(s) 275, 385, 1301
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 2 cut(s) 509, 897
SmoI CTYRAG 2 cut(s) 509, 897
SphI GCATGC 1 cut(s) 437
Sse9I AATT 7 cut(s) 334, 399, 442, 514, 720, 977, 1079
SspMI CTAG 3 cut(s) 488, 1227, 1601
StyD4I CCNGG 5 cut(s) 281, 754, 886, 931, 1253
StyI CCWWGG 1 cut(s) 142
TaaI ACNGT 4 cut(s) 1002, 1188, 1196, 1298
TaqI TCGA 5 cut(s) 99, 246, 311, 522, 623
TaqII GACCGA 1 cut(s) 292
TasI AATT 7 cut(s) 334, 399, 442, 514, 720, 977, 1079
TauI GCSGC 6 cut(s) 141, 254, 504, 692, 1015, 1575
TfiI GAWTC 6 cut(s) 169, 205, 350, 601, 650, 1283
Tru1I TTAA 2 cut(s) 564, 1056
Tru9I TTAA 2 cut(s) 564, 1056
TscAI CASTG 4 cut(s) 966, 1099, 1243, 1387
TseFI GTSAC 2 cut(s) 1101, 1169
TseI GCWGC 7 cut(s) 25, 28, 31, 317, 940, 1024, 1547
Tsp45I GTSAC 2 cut(s) 1101, 1169
TspDTI ATGAA 6 cut(s) 43, 80, 440, 713, 749, 1452
TspGWI ACGGA 1 cut(s) 222
TspRI CASTG 4 cut(s) 966, 1099, 1243, 1387
Van91I CCANNNNNTGG 2 cut(s) 288, 887
VneI GTGCAC 1 cut(s) 471
VpaK11BI GGWCC 3 cut(s) 275, 385, 1301
XagI CCTNNNNNAGG 1 cut(s) 1080
XapI RAATTY 2 cut(s) 514, 720
XceI RCATGY 4 cut(s) 437, 455, 812, 1424
XcmI CCANNNNNNNNNTGG 1 cut(s) 1641
XmiI GTMKAC 1 cut(s) 538
XspI CTAG 3 cut(s) 488, 1227, 1601
Zsp2I ATGCAT 1 cut(s) 1213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.