Rh2AG355300

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0004149
Physical Location & Seq
Forward (+)
52352980 .. 52355370
2391 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG355300.1

Sequence Viewer

Length: 1701 bp
ATGAATACTACATCCTTCATGGACAAGCAGATAATGGACCTGTCACAGGGATCGTCACAGCACAACAACGACTTCCTTGACCTGATGAACAATTCCCAAGAGGAAGAGCACCAGGTGGGTCATGGAAATGGGATTTCCAAGAAGGAAGAGATTTTGCCGAACTATGATTTCCAGCCCATCCGTCCCATCACTGGAGCCTCTTCGCAATCCCCAAGTTTTGATGCTACACCTAATCTCGGAGGTGGAGGAGGATCCACTAGGGCTTGGAACTCCCAAGAGTCCAAGTCCAACACCAATGCTCCTATCAGAAATTATGGTTCTGTGGATTCCTTCGAACGTGCAAAAGATGTTGTGGAGAAGGACCGACATGTTCCTGATGCTACAATTATATCAGAGATTGACCACACCATGAAGAAGTATGCTGATAATTTACTTCAAGTTATGGAAGGTATTAGTGCACGACTAACACAACTTGAGAGCAGAACCCGCCACCTTGAGAATTCTGTTGATGATTTGAAGGTATCTGTGGGAAACAATCATGGAAATGCTGATGGAAAGATGAGACAAATGGAGAATATTCTTAGAGATGTGCAAACAGGTGTTCAGGATTTGAAGGATAAGCAATCAATAGTAGAGGCTCAGCTGCAGCTTGGGAAGATACAGCTATCAAATCCCAAGGTAGATCCTCAATTGGAATCTCGGAATGCTCTGAATGCAGATTCTGGGCATAAAGTAGCCTCTGCTCCTCAACAATCTCACCAACAGCTTCCTCCTGTGAACATTCCACCATCACTTCCTGCTGTTTCTCCACCAAATGCTCCTCCACAGCCTATGCTCCAAAGTGTACCACCTTCAATTCAGCATCCAAATCAGTACTCTCAGAACCAGATCCCTCATGTTCCTCAGCGAGACCAATATTTCCCAGCTGCACCTGTTCAAACTCAAGAAGCTCCAAATCAGCAATACCAATTGCCTGCAGGTCAGCAGTCACTCCCCCCTCCTACGGTACCTCCGCATCAACAATTTCAGCCTACCAGTCAACCACAGTATCCTCAGCCACCGCCCCAGCTTCCTCAACAGCACCATTCACTTCCACCTGTTAATCCCTCTCAACTCCAGCCTACCCTAGGTCACCATGCTGAAGATACTCCTTATGTTCCTTCCCAGACCTACCCACCCAGTCTTCACCAGCCACCTTCTCAGACACCTAGTGGGCCTCCTCCCTCCCAACAATATTATAGTCCAACATCCAATGTATATGAGCCACCATCTAGCAGACCTAATTCAGGGTATTCCTCTGGGTACCCTCCTTCTGGGCTTAATGAACCGTATCATTATGGTGGATCACCTTCTCAATATGGTGGTACCTCCTCGATGAAACCACAACTCTCTTCTGCTACTGCTCAAAGTCAAAGTGGAGGAAGTGGTTACCCACAGCTCCCAACAGCTAGGGTATTACCACATGCAGTGCCTACCGCCTCTGGTGTAAGTGATCGATCAGGTTCAGCTGGGACTGGAAACAAGGTTCCTATTGATGATGTGATTGAAAGAGTGACAAGCATGGGTTTTTCGAGAGACCATGTAAGGGCAACAGTTCGGAAGCTGACAGACAACGGTCAGGCAGTTGACCTGAATGTTGTGTTAGATAAGCTGATGAATGATGGGGATGTCCAGCCACCTAGAGGTTGGTTTGGTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
DUF1421

Protein Analysis

566

Amino Acids

61.52

Weight (kDa)

5.97

Isoelectric Point (pI)

65.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 511 - 554 1.6e-19 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 968
Acc65I GGTACC 3 cut(s) 1006, 1302, 1364
AccB1I GGYRCC 3 cut(s) 1006, 1302, 1364
AciI CCGC 4 cut(s) 487, 1013, 1061, 1476
AclWI GGATC 6 cut(s) 58, 246, 259, 677, 883, 1351
AcsI RAATTY 1 cut(s) 499
AcuI CTGAAG 1 cut(s) 1161
AdeI CACNNNGTG 2 cut(s) 115, 1211
AfaI GTAC 5 cut(s) 846, 875, 1008, 1304, 1366
AfiI CCNNNNNNNGG 9 cut(s) 46, 191, 236, 1003, 1127, 1286, 1313, 1585, 1682
AflIII ACRYGT 1 cut(s) 367
AgsI TTSAA 6 cut(s) 437, 517, 613, 855, 938, 1547
AjnI CCWGG 1 cut(s) 111
AloI GAACNNNNNNTCC 2 cut(s) 1509, 1541
Alw21I GWGCWC 2 cut(s) 111, 460
Alw26I GTCTC 3 cut(s) 556, 903, 1569
Alw44I GTGCAC 1 cut(s) 456
AlwI GGATC 6 cut(s) 58, 246, 259, 677, 883, 1351
AlwNI CAGNNNCTG 1 cut(s) 722
AoxI GGCC 1 cut(s) 1214
ApaLI GTGCAC 1 cut(s) 456
ApeKI GCWGC 3 cut(s) 643, 646, 926
ApoI RAATTY 1 cut(s) 499
Asp718I GGTACC 3 cut(s) 1006, 1302, 1364
AspA2I CCTAGG 1 cut(s) 1126
AspS9I GGNCC 3 cut(s) 37, 361, 1214
AsuHPI GGTGA 4 cut(s) 749, 1124, 1178, 1338
AsuII TTCGAA 1 cut(s) 333
AvaII GGWCC 2 cut(s) 37, 361
AvrII CCTAGG 1 cut(s) 1126
BaeGI GKGCMC 1 cut(s) 460
BaeI ACNNNNGTAYC 2 cut(s) 1031, 1064
BamHI GGATCC 1 cut(s) 251
BanI GGYRCC 3 cut(s) 1006, 1302, 1364
BbsI GAAGAC 1 cut(s) 1175
Bbv12I GWGCWC 2 cut(s) 111, 460
BbvCI CCTCAGC 2 cut(s) 903, 1053
BbvI GCAGC 3 cut(s) 630, 658, 913
BccI CCATC 6 cut(s) 185, 194, 545, 796, 1276, 1655
BciT130I CCWGG 1 cut(s) 113
BciVI GTATCC 1 cut(s) 1059
BcoDI GTCTC 3 cut(s) 556, 903, 1569
BfaI CTAG 6 cut(s) 258, 1127, 1209, 1272, 1449, 1680
BfmI CTRYAG 2 cut(s) 644, 975
BfuAI ACCTGC 1 cut(s) 968
BfuI GTATCC 1 cut(s) 1059
BisI GCNGC 3 cut(s) 644, 647, 927
BlnI CCTAGG 1 cut(s) 1126
BlpI GCTNAGC 1 cut(s) 639
BlsI GCNGC 3 cut(s) 645, 648, 928
BmcAI AGTACT 1 cut(s) 875
Bme1390I CCNGG 1 cut(s) 113
Bme18I GGWCC 2 cut(s) 37, 361
BmgT120I GGNCC 3 cut(s) 37, 361, 1214
BmiI GGNNCC 6 cut(s) 196, 253, 1008, 1304, 1366, 1527
BmrFI CCNGG 1 cut(s) 113
BmrI ACTGGG 1 cut(s) 1173
BmsI GCATC 4 cut(s) 211, 367, 871, 1024
BmuI ACTGGG 1 cut(s) 1173
BoxI GACNNNNGTC 1 cut(s) 1614
BpiI GAAGAC 1 cut(s) 1175
BpmI CTGGAG 2 cut(s) 213, 1100
Bpu10I CCTNAGC 2 cut(s) 903, 1053
Bpu1102I GCTNAGC 1 cut(s) 639
Bpu14I TTCGAA 1 cut(s) 333
BpuEI CTTGAG 3 cut(s) 494, 515, 927
Bsa29I ATCGAT 1 cut(s) 1495
BsaI GGTCTC 2 cut(s) 903, 1569
BsaJI CCNNGG 2 cut(s) 675, 1126
BsaXI ACNNNNNCTCC 4 cut(s) 283, 313, 994, 1024
Bsc4I CCNNNNNNNGG 9 cut(s) 46, 191, 236, 1003, 1127, 1286, 1313, 1585, 1682
Bse1I ACTGG 4 cut(s) 196, 1035, 1179, 1519
BseBI CCWGG 1 cut(s) 113
BseCI ATCGAT 1 cut(s) 1495
BseDI CCNNGG 2 cut(s) 675, 1126
BseGI GGATG 5 cut(s) 11, 177, 862, 1247, 1672
BseLI CCNNNNNNNGG 9 cut(s) 46, 191, 236, 1003, 1127, 1286, 1313, 1585, 1682
BseMII CTCAG 5 cut(s) 653, 893, 917, 1067, 1214
BseNI ACTGG 4 cut(s) 196, 1035, 1179, 1519
BseRI GAGGAG 5 cut(s) 261, 735, 810, 1209, 1360
BseSI GKGCMC 1 cut(s) 460
BseXI GCAGC 3 cut(s) 630, 658, 913
BseYI CCCAGC 3 cut(s) 922, 1065, 1508
BsgI GTGCAG 1 cut(s) 912
BshFI GGCC 1 cut(s) 1216
BshNI GGYRCC 3 cut(s) 1006, 1302, 1364
BshVI ATCGAT 1 cut(s) 1495
BsiHKAI GWGCWC 2 cut(s) 111, 460
BslFI GGGAC 2 cut(s) 168, 1525
BslI CCNNNNNNNGG 9 cut(s) 46, 191, 236, 1003, 1127, 1286, 1313, 1585, 1682
BsmAI GTCTC 3 cut(s) 556, 903, 1569
BsmFI GGGAC 2 cut(s) 168, 1525
BsmI GAATGC 2 cut(s) 709, 718
BsnI GGCC 1 cut(s) 1216
Bso31I GGTCTC 2 cut(s) 903, 1569
Bsp119I TTCGAA 1 cut(s) 333
Bsp1286I GDGCHC 2 cut(s) 111, 460
Bsp143I GATC 7 cut(s) 50, 251, 682, 888, 1343, 1492, 1496
Bsp1720I GCTNAGC 1 cut(s) 639
BspACI CCGC 4 cut(s) 487, 1013, 1061, 1476
BspANI GGCC 1 cut(s) 1216
BspCNI CTCAG 5 cut(s) 652, 892, 916, 1066, 1213
BspDI ATCGAT 1 cut(s) 1495
BspLI GGNNCC 6 cut(s) 196, 253, 1008, 1304, 1366, 1527
BspMAI CTGCAG 2 cut(s) 648, 979
BspMI ACCTGC 1 cut(s) 968
BspPI GGATC 6 cut(s) 58, 246, 259, 677, 883, 1351
BspQI GCTCTTC 1 cut(s) 99
BspT104I TTCGAA 1 cut(s) 333
BspT107I GGYRCC 3 cut(s) 1006, 1302, 1364
BspTNI GGTCTC 2 cut(s) 903, 1569
BsrI ACTGG 4 cut(s) 196, 1035, 1179, 1519
BssECI CCNNGG 2 cut(s) 675, 1126
BssMI GATC 7 cut(s) 50, 251, 682, 888, 1343, 1492, 1496
BssT1I CCWWGG 2 cut(s) 675, 1126
Bst2UI CCWGG 1 cut(s) 113
Bst4CI ACNGT 5 cut(s) 1006, 1047, 1329, 1594, 1616
Bst6I CTCTTC 4 cut(s) 99, 141, 205, 1396
BstBI TTCGAA 1 cut(s) 333
BstC8I GCNNGC 1 cut(s) 975
BstDEI CTNAG 6 cut(s) 581, 639, 879, 903, 1053, 1200
BstEII GGTNACC 2 cut(s) 1130, 1427
BstENI CCTNNNNNAGG 3 cut(s) 44, 1125, 1284
BstF5I GGATG 5 cut(s) 11, 177, 862, 1247, 1672
BstKTI GATC 7 cut(s) 53, 254, 685, 891, 1346, 1495, 1499
BstMAI GTCTC 3 cut(s) 556, 903, 1569
BstMBI GATC 7 cut(s) 50, 251, 682, 888, 1343, 1492, 1496
BstMWI GCNNNNNNNGC 2 cut(s) 486, 713
BstNI CCWGG 1 cut(s) 113
BstNSI RCATGY 2 cut(s) 371, 1466
BstPAI GACNNNNGTC 1 cut(s) 1614
BstPI GGTNACC 2 cut(s) 1130, 1427
BstSCI CCNGG 1 cut(s) 111
BstSFI CTRYAG 2 cut(s) 644, 975
BstSLI GKGCMC 1 cut(s) 460
BstV1I GCAGC 3 cut(s) 630, 658, 913
BstV2I GAAGAC 1 cut(s) 1175
BstX2I RGATCY 3 cut(s) 251, 682, 888
BstYI RGATCY 3 cut(s) 251, 682, 888
Bsu15I ATCGAT 1 cut(s) 1495
BsuI GTATCC 1 cut(s) 1059
BsuRI GGCC 1 cut(s) 1216
BsuTUI ATCGAT 1 cut(s) 1495
BtsCI GGATG 5 cut(s) 11, 177, 862, 1247, 1672
BtsI GCAGTG 1 cut(s) 1473
BtsIMutI CAGTG 2 cut(s) 189, 1473
BveI ACCTGC 1 cut(s) 968
Cac8I GCNNGC 1 cut(s) 975
CaiI CAGNNNCTG 1 cut(s) 722
Cfr13I GGNCC 3 cut(s) 37, 361, 1214
ClaI ATCGAT 1 cut(s) 1495
CsiI ACCWGGT 1 cut(s) 111
Csp6I GTAC 5 cut(s) 845, 874, 1007, 1303, 1365
CviQI GTAC 5 cut(s) 845, 874, 1007, 1303, 1365
DdeI CTNAG 6 cut(s) 581, 639, 879, 903, 1053, 1200
DpnI GATC 7 cut(s) 52, 253, 684, 890, 1345, 1494, 1498
DpnII GATC 7 cut(s) 50, 251, 682, 888, 1343, 1492, 1496
DraIII CACNNNGTG 2 cut(s) 115, 1211
Eam1104I CTCTTC 4 cut(s) 99, 141, 205, 1396
EarI CTCTTC 4 cut(s) 99, 141, 205, 1396
Eco130I CCWWGG 2 cut(s) 675, 1126
Eco31I GGTCTC 2 cut(s) 903, 1569
Eco47I GGWCC 2 cut(s) 37, 361
Eco57I CTGAAG 1 cut(s) 1161
Eco91I GGTNACC 2 cut(s) 1130, 1427
EcoNI CCTNNNNNAGG 3 cut(s) 44, 1125, 1284
EcoO65I GGTNACC 2 cut(s) 1130, 1427
EcoRI GAATTC 1 cut(s) 499
EcoRII CCWGG 1 cut(s) 111
EcoT14I CCWWGG 2 cut(s) 675, 1126
ErhI CCWWGG 2 cut(s) 675, 1126
FaqI GGGAC 2 cut(s) 168, 1525
FauI CCCGC 1 cut(s) 494
Fnu4HI GCNGC 3 cut(s) 644, 647, 927
FokI GGATG 4 cut(s) 164, 849, 1234, 1679
Fsp4HI GCNGC 3 cut(s) 644, 647, 927
FspBI CTAG 6 cut(s) 258, 1127, 1209, 1272, 1449, 1680
GluI GCNGC 3 cut(s) 644, 647, 927
GsaI CCCAGC 3 cut(s) 926, 1069, 1512
GsuI CTGGAG 2 cut(s) 213, 1100
HaeIII GGCC 1 cut(s) 1216
HincII GTYRAC 2 cut(s) 1040, 1627
HindII GTYRAC 2 cut(s) 1040, 1627
HinfI GANTC 4 cut(s) 278, 326, 695, 719
HphI GGTGA 4 cut(s) 749, 1124, 1178, 1338
Hpy166II GTNNAC 5 cut(s) 458, 778, 845, 1040, 1627
Hpy188I TCNGA 8 cut(s) 239, 308, 394, 702, 711, 882, 1203, 1599
Hpy188III TCNNGA 4 cut(s) 374, 605, 944, 1572
Hpy8I GTNNAC 5 cut(s) 458, 778, 845, 1040, 1627
HpyCH4III ACNGT 5 cut(s) 1006, 1047, 1329, 1594, 1616
HpyCH4IV ACGT 1 cut(s) 337
HpyCH4V TGCA 8 cut(s) 341, 458, 592, 646, 716, 929, 977, 1466
HpyF10VI GCNNNNNNNGC 2 cut(s) 486, 713
HpyF3I CTNAG 6 cut(s) 581, 639, 879, 903, 1053, 1200
HpySE526I ACGT 1 cut(s) 337
KpnI GGTACC 3 cut(s) 1010, 1306, 1368
Kzo9I GATC 7 cut(s) 50, 251, 682, 888, 1343, 1492, 1496
LguI GCTCTTC 1 cut(s) 99
LmnI GCTCC 7 cut(s) 194, 304, 748, 823, 840, 955, 1443
Lsp1109I GCAGC 3 cut(s) 630, 658, 913
LweI GCATC 4 cut(s) 211, 367, 871, 1024
MabI ACCWGGT 1 cut(s) 111
MaeI CTAG 6 cut(s) 258, 1127, 1209, 1272, 1449, 1680
MaeII ACGT 1 cut(s) 337
MaeIII GTNAC 6 cut(s) 42, 54, 987, 1130, 1427, 1552
MalI GATC 7 cut(s) 52, 253, 684, 890, 1345, 1494, 1498
MboI GATC 7 cut(s) 50, 251, 682, 888, 1343, 1492, 1496
MboII GAAGA 8 cut(s) 116, 158, 192, 424, 667, 1154, 1175, 1383
MfeI CAATTG 2 cut(s) 689, 968
MflI RGATCY 3 cut(s) 251, 682, 888
MhlI GDGCHC 2 cut(s) 111, 460
MlyI GAGTC 1 cut(s) 287
MmeI TCCRAC 2 cut(s) 312, 1268
MseI TTAA 2 cut(s) 1101, 1320
MslI CAYNNNNRTG 3 cut(s) 126, 543, 1338
MspA1I CMGCKG 3 cut(s) 643, 926, 1508
MspR9I CCNGG 1 cut(s) 113
MunI CAATTG 2 cut(s) 689, 968
Mva1269I GAATGC 2 cut(s) 709, 718
MvaI CCWGG 1 cut(s) 113
MwoI GCNNNNNNNGC 2 cut(s) 486, 713
NdeII GATC 7 cut(s) 50, 251, 682, 888, 1343, 1492, 1496
NlaIV GGNNCC 6 cut(s) 196, 253, 1008, 1304, 1366, 1527
NmuCI GTSAC 5 cut(s) 42, 54, 987, 1130, 1552
NspI RCATGY 2 cut(s) 371, 1466
NspV TTCGAA 1 cut(s) 333
PciI ACATGT 1 cut(s) 367
PciSI GCTCTTC 1 cut(s) 99
PctI GAATGC 2 cut(s) 709, 718
PfeI GAWTC 3 cut(s) 326, 695, 719
PkrI GCNGC 3 cut(s) 645, 648, 928
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
PscI ACATGT 1 cut(s) 367
PshAI GACNNNNGTC 1 cut(s) 1614
Psp6I CCWGG 1 cut(s) 111
PspEI GGTNACC 2 cut(s) 1130, 1427
PspFI CCCAGC 3 cut(s) 922, 1065, 1508
PspGI CCWGG 1 cut(s) 111
PspN4I GGNNCC 6 cut(s) 196, 253, 1008, 1304, 1366, 1527
PspPI GGNCC 3 cut(s) 37, 361, 1214
PstI CTGCAG 2 cut(s) 648, 979
PstNI CAGNNNCTG 1 cut(s) 722
PsuI RGATCY 3 cut(s) 251, 682, 888
PvuII CAGCTG 3 cut(s) 643, 926, 1508
RsaI GTAC 5 cut(s) 846, 875, 1008, 1304, 1366
RsaNI GTAC 5 cut(s) 845, 874, 1007, 1303, 1365
RseI CAYNNNNRTG 3 cut(s) 126, 543, 1338
SapI GCTCTTC 1 cut(s) 99
SaqAI TTAA 2 cut(s) 1101, 1320
SatI GCNGC 3 cut(s) 644, 647, 927
Sau3AI GATC 7 cut(s) 50, 251, 682, 888, 1343, 1492, 1496
Sau96I GGNCC 3 cut(s) 37, 361, 1214
SbfI CCTGCAGG 1 cut(s) 979
ScaI AGTACT 1 cut(s) 875
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 1 cut(s) 113
SdaI CCTGCAGG 1 cut(s) 979
SduI GDGCHC 2 cut(s) 111, 460
SexAI ACCWGGT 1 cut(s) 111
SfaNI GCATC 4 cut(s) 211, 367, 871, 1024
SfcI CTRYAG 2 cut(s) 644, 975
SfuI TTCGAA 1 cut(s) 333
SinI GGWCC 2 cut(s) 37, 361
SmiMI CAYNNNNRTG 3 cut(s) 126, 543, 1338
SmlI CTYRAG 3 cut(s) 473, 494, 942
SmoI CTYRAG 3 cut(s) 473, 494, 942
Sse8387I CCTGCAGG 1 cut(s) 979
SsiI CCGC 4 cut(s) 487, 1013, 1061, 1476
SspI AATATT 3 cut(s) 577, 917, 1235
SspMI CTAG 6 cut(s) 258, 1127, 1209, 1272, 1449, 1680
StyD4I CCNGG 1 cut(s) 111
StyI CCWWGG 2 cut(s) 675, 1126
TaaI ACNGT 5 cut(s) 1006, 1047, 1329, 1594, 1616
TaiI ACGT 1 cut(s) 340
TaqI TCGA 4 cut(s) 333, 1373, 1495, 1571
TaqII GACCGA 1 cut(s) 378
TatI WGTACW 1 cut(s) 873
TfiI GAWTC 3 cut(s) 326, 695, 719
Tru1I TTAA 2 cut(s) 1101, 1320
Tru9I TTAA 2 cut(s) 1101, 1320
TscAI CASTG 2 cut(s) 196, 1473
TseFI GTSAC 5 cut(s) 42, 54, 987, 1130, 1552
TseI GCWGC 3 cut(s) 643, 646, 926
Tsp45I GTSAC 5 cut(s) 42, 54, 987, 1130, 1552
TspDTI ATGAA 7 cut(s) 7, 17, 101, 425, 1338, 1391, 1670
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 2 cut(s) 196, 1473
VneI GTGCAC 1 cut(s) 456
VpaK11BI GGWCC 2 cut(s) 37, 361
XagI CCTNNNNNAGG 3 cut(s) 44, 1125, 1284
XapI RAATTY 1 cut(s) 499
XceI RCATGY 2 cut(s) 371, 1466
XcmI CCANNNNNNNNNTGG 2 cut(s) 119, 1683
XmaJI CCTAGG 1 cut(s) 1126
XspI CTAG 6 cut(s) 258, 1127, 1209, 1272, 1449, 1680
ZrmI AGTACT 1 cut(s) 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.