Rh2CG338900

UBA-like domain (DUF1421)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0004149
Physical Location & Seq
Forward (+)
45081105 .. 45085826
4722 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG338900.1

Sequence Viewer

Length: 1428 bp
ATGGTGATAGTGTTTCCATTTTTTTGTTTTATACAGAATTATGGTTCTGTGGATTCCTTCGAACGTGCAAAAGATGTTGTGGAGAAGGACCGACATGTTCCTGATGCTACAACTATATCAGAGATTGACCACACCATGAAGAAGTATGCTGATAATTTACTTCAAGTTATGGAAGGTATTAGTGCACGACTAACACAACTTGAGAGCAGAACCCGCCACCTTGAGAATTCTGTTGATGATTTGAAGGTATCTGTGGGAAACAATCATGGAAATGCTGATGGAAAGATGAGACAAATGGAGAATATTCTTAGAGATGTGCAAACAGGTGTTCAGGATTTGAAGGATAAGCAATCAATAGTAGAGGCTCAGTTGCAGCTTGGGAAGATACAGCTATCAAATCCCAAGGTAGATCCTCAATTGGAATCTCGGAATGCTCTGAATGCAGATTCTGGGCATAAAGTAGCCTCTGCTCCTCAACAATCTCACCAACAGCTTCCTCCTGTGAACATTCCACCATCACTTCCTGCTGTTTCTCCACCAAATGCTCCTCCACAGCCTATGCTCCAAAGTATACCTCCTTCAATTCAGCATCCAAATCAGTACTCTCAGAACCAGATCCCTCCTGTTCCTCAGCGAGACCAATATTTCCCAGCTGCACCTGTTCAAACTCAAGAAGCTCCAAATCAGCAATACCAATTGCCTGCAGGTCAGCAGTCACTCCCCCCTCCTACGGTACCTCCGCATCAACAATTTCAGCCTACCAGTCAACCACAGTATCCTCAGCCACCGCCCCAGCTTCCTCAACAGCACCATTCACTTCCACCTGTTAATCCCTCTCAACTCCAGCCTACCCTAGGTCACCATGCTGAAGATACTCCTTATGTTCCTTCCCAGACCTACCCACCCAGTCTTCGCCAGCCACCTTCTCAGACACCTAGTGGGCCTCCTCCCTCCCAACAATATTATAGTCCAACATCCAATGTATATGAACCACCATCTAGCAGACCTAATTCAGGGTATTCCTCTGGGTACCCTCCTTCTGGGCTTAATGAACCGTATCATTATGGTGGATCACCTTCTCAATATGGTGGTACCTCCTCGATGAAACCACAACTCTCTTCTGCTACTGCTCAAAGTCAAAGTGGAGGAAGTGGTTACCCACAGCTGCCAACAGCTAGGGTATTACCACATGCAGTGCCTACCGCCTCTGGTGTAAGTGATCGATCAGGTTCAGCTGGGACTGGAAACAAGGTTCCTATTGATGATGTGATTGAAAGAGTGACAAGCATGGGTTTTCCGAGAGACCATGTAAGGGCAACAGTTCGGAAGCTGACAGACAACGGTCAGGCAGTTGACCTGAATGTTGTGTTAGATAAGCTGATGAATGATGGGGATGTCCAGCCACCTAGAGGTTGGTTTGGTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00380 ko01100 map00380 map01100
Pfam Domains
Protein Families
DUF1421

Protein Analysis

475

Amino Acids

51.78

Weight (kDa)

6.3

Isoelectric Point (pI)

66.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1421 PF07223 420 - 463 2.5e-20 UBA-like domain (DUF1421)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 695
Acc65I GGTACC 3 cut(s) 733, 1029, 1091
AccB1I GGYRCC 3 cut(s) 733, 1029, 1091
AccI GTMKAC 1 cut(s) 571
AciI CCGC 4 cut(s) 214, 740, 788, 1203
AclWI GGATC 3 cut(s) 404, 610, 1078
AcsI RAATTY 1 cut(s) 226
AcuI CTGAAG 1 cut(s) 888
AdeI CACNNNGTG 1 cut(s) 938
AfaI GTAC 4 cut(s) 602, 735, 1031, 1093
AfiI CCNNNNNNNGG 6 cut(s) 730, 854, 1013, 1040, 1312, 1409
AflIII ACRYGT 1 cut(s) 94
AgsI TTSAA 6 cut(s) 164, 244, 340, 582, 665, 1274
AloI GAACNNNNNNTCC 2 cut(s) 1236, 1268
Alw21I GWGCWC 1 cut(s) 187
Alw26I GTCTC 3 cut(s) 283, 630, 1296
Alw44I GTGCAC 1 cut(s) 183
AlwI GGATC 3 cut(s) 404, 610, 1078
AlwNI CAGNNNCTG 1 cut(s) 449
AoxI GGCC 1 cut(s) 941
ApaLI GTGCAC 1 cut(s) 183
ApeKI GCWGC 3 cut(s) 373, 653, 1165
ApoI RAATTY 1 cut(s) 226
Asp718I GGTACC 3 cut(s) 733, 1029, 1091
AspA2I CCTAGG 1 cut(s) 853
AspS9I GGNCC 2 cut(s) 88, 941
AsuHPI GGTGA 4 cut(s) 16, 476, 851, 1065
AsuII TTCGAA 1 cut(s) 60
AvaII GGWCC 1 cut(s) 88
AvrII CCTAGG 1 cut(s) 853
BaeGI GKGCMC 1 cut(s) 187
BaeI ACNNNNGTAYC 2 cut(s) 758, 791
BanI GGYRCC 3 cut(s) 733, 1029, 1091
BarI GAAGNNNNNNTAC 2 cut(s) 562, 594
BbsI GAAGAC 1 cut(s) 902
Bbv12I GWGCWC 1 cut(s) 187
BbvCI CCTCAGC 2 cut(s) 630, 780
BbvI GCAGC 3 cut(s) 385, 640, 1152
BccI CCATC 4 cut(s) 272, 523, 1003, 1382
BciVI GTATCC 1 cut(s) 786
BcoDI GTCTC 3 cut(s) 283, 630, 1296
BfaI CTAG 5 cut(s) 854, 936, 999, 1176, 1407
BfmI CTRYAG 1 cut(s) 702
BfuAI ACCTGC 1 cut(s) 695
BfuI GTATCC 1 cut(s) 786
BisI GCNGC 3 cut(s) 374, 654, 1166
BlnI CCTAGG 1 cut(s) 853
BlsI GCNGC 3 cut(s) 375, 655, 1167
BmcAI AGTACT 1 cut(s) 602
Bme18I GGWCC 1 cut(s) 88
BmgT120I GGNCC 2 cut(s) 88, 941
BmiI GGNNCC 4 cut(s) 735, 1031, 1093, 1254
BmrI ACTGGG 1 cut(s) 900
BmsI GCATC 3 cut(s) 94, 598, 751
BmuI ACTGGG 1 cut(s) 900
BoxI GACNNNNGTC 1 cut(s) 1341
BpiI GAAGAC 1 cut(s) 902
BpmI CTGGAG 1 cut(s) 827
Bpu10I CCTNAGC 2 cut(s) 630, 780
Bpu14I TTCGAA 1 cut(s) 60
BpuEI CTTGAG 3 cut(s) 221, 242, 654
Bsa29I ATCGAT 1 cut(s) 1222
BsaI GGTCTC 2 cut(s) 630, 1296
BsaJI CCNNGG 2 cut(s) 402, 853
BsaXI ACNNNNNCTCC 2 cut(s) 721, 751
Bsc4I CCNNNNNNNGG 6 cut(s) 730, 854, 1013, 1040, 1312, 1409
Bse1I ACTGG 3 cut(s) 762, 906, 1246
BseCI ATCGAT 1 cut(s) 1222
BseDI CCNNGG 2 cut(s) 402, 853
BseGI GGATG 3 cut(s) 589, 974, 1399
BseLI CCNNNNNNNGG 6 cut(s) 730, 854, 1013, 1040, 1312, 1409
BseMII CTCAG 5 cut(s) 380, 620, 644, 794, 941
BseNI ACTGG 3 cut(s) 762, 906, 1246
BseRI GAGGAG 4 cut(s) 462, 537, 936, 1087
BseSI GKGCMC 1 cut(s) 187
BseXI GCAGC 3 cut(s) 385, 640, 1152
BseYI CCCAGC 3 cut(s) 649, 792, 1235
BsgI GTGCAG 1 cut(s) 639
BshFI GGCC 1 cut(s) 943
BshNI GGYRCC 3 cut(s) 733, 1029, 1091
BshVI ATCGAT 1 cut(s) 1222
BsiHKAI GWGCWC 1 cut(s) 187
BslFI GGGAC 1 cut(s) 1252
BslI CCNNNNNNNGG 6 cut(s) 730, 854, 1013, 1040, 1312, 1409
BsmAI GTCTC 3 cut(s) 283, 630, 1296
BsmFI GGGAC 1 cut(s) 1252
BsmI GAATGC 2 cut(s) 436, 445
BsnI GGCC 1 cut(s) 943
Bso31I GGTCTC 2 cut(s) 630, 1296
Bsp119I TTCGAA 1 cut(s) 60
Bsp1286I GDGCHC 1 cut(s) 187
Bsp143I GATC 5 cut(s) 409, 615, 1070, 1219, 1223
BspACI CCGC 4 cut(s) 214, 740, 788, 1203
BspANI GGCC 1 cut(s) 943
BspCNI CTCAG 5 cut(s) 379, 619, 643, 793, 940
BspDI ATCGAT 1 cut(s) 1222
BspLI GGNNCC 4 cut(s) 735, 1031, 1093, 1254
BspMAI CTGCAG 1 cut(s) 706
BspMI ACCTGC 1 cut(s) 695
BspPI GGATC 3 cut(s) 404, 610, 1078
BspT104I TTCGAA 1 cut(s) 60
BspT107I GGYRCC 3 cut(s) 733, 1029, 1091
BspTNI GGTCTC 2 cut(s) 630, 1296
BsrI ACTGG 3 cut(s) 762, 906, 1246
BssECI CCNNGG 2 cut(s) 402, 853
BssMI GATC 5 cut(s) 409, 615, 1070, 1219, 1223
BssNAI GTATAC 1 cut(s) 572
BssT1I CCWWGG 2 cut(s) 402, 853
Bst1107I GTATAC 1 cut(s) 572
Bst4CI ACNGT 5 cut(s) 733, 774, 1056, 1321, 1343
Bst6I CTCTTC 1 cut(s) 1123
BstBI TTCGAA 1 cut(s) 60
BstC8I GCNNGC 2 cut(s) 702, 917
BstDEI CTNAG 6 cut(s) 308, 366, 606, 630, 780, 927
BstEII GGTNACC 2 cut(s) 857, 1154
BstENI CCTNNNNNAGG 2 cut(s) 852, 1011
BstF5I GGATG 3 cut(s) 589, 974, 1399
BstKTI GATC 5 cut(s) 412, 618, 1073, 1222, 1226
BstMAI GTCTC 3 cut(s) 283, 630, 1296
BstMBI GATC 5 cut(s) 409, 615, 1070, 1219, 1223
BstMWI GCNNNNNNNGC 2 cut(s) 213, 440
BstNSI RCATGY 2 cut(s) 98, 1193
BstPAI GACNNNNGTC 1 cut(s) 1341
BstPI GGTNACC 2 cut(s) 857, 1154
BstSFI CTRYAG 1 cut(s) 702
BstSLI GKGCMC 1 cut(s) 187
BstV1I GCAGC 3 cut(s) 385, 640, 1152
BstV2I GAAGAC 1 cut(s) 902
BstX2I RGATCY 2 cut(s) 409, 615
BstYI RGATCY 2 cut(s) 409, 615
BstZ17I GTATAC 1 cut(s) 572
Bsu15I ATCGAT 1 cut(s) 1222
BsuI GTATCC 1 cut(s) 786
BsuRI GGCC 1 cut(s) 943
BsuTUI ATCGAT 1 cut(s) 1222
BtsCI GGATG 3 cut(s) 589, 974, 1399
BtsI GCAGTG 1 cut(s) 1200
BtsIMutI CAGTG 1 cut(s) 1200
BveI ACCTGC 1 cut(s) 695
Cac8I GCNNGC 2 cut(s) 702, 917
CaiI CAGNNNCTG 1 cut(s) 449
Cfr13I GGNCC 2 cut(s) 88, 941
ClaI ATCGAT 1 cut(s) 1222
Csp6I GTAC 4 cut(s) 601, 734, 1030, 1092
CviAII CATG 7 cut(s) 95, 136, 266, 863, 1190, 1288, 1307
CviQI GTAC 4 cut(s) 601, 734, 1030, 1092
DdeI CTNAG 6 cut(s) 308, 366, 606, 630, 780, 927
DpnI GATC 5 cut(s) 411, 617, 1072, 1221, 1225
DpnII GATC 5 cut(s) 409, 615, 1070, 1219, 1223
DraIII CACNNNGTG 1 cut(s) 938
Eam1104I CTCTTC 1 cut(s) 1123
EarI CTCTTC 1 cut(s) 1123
Eco130I CCWWGG 2 cut(s) 402, 853
Eco31I GGTCTC 2 cut(s) 630, 1296
Eco47I GGWCC 1 cut(s) 88
Eco57I CTGAAG 1 cut(s) 888
Eco91I GGTNACC 2 cut(s) 857, 1154
EcoNI CCTNNNNNAGG 2 cut(s) 852, 1011
EcoO65I GGTNACC 2 cut(s) 857, 1154
EcoRI GAATTC 1 cut(s) 226
EcoT14I CCWWGG 2 cut(s) 402, 853
ErhI CCWWGG 2 cut(s) 402, 853
FaeI CATG 7 cut(s) 98, 139, 269, 866, 1193, 1291, 1310
FaqI GGGAC 1 cut(s) 1252
FatI CATG 7 cut(s) 94, 135, 265, 862, 1189, 1287, 1306
FauI CCCGC 1 cut(s) 221
FblI GTMKAC 1 cut(s) 571
Fnu4HI GCNGC 3 cut(s) 374, 654, 1166
FokI GGATG 3 cut(s) 576, 961, 1406
Fsp4HI GCNGC 3 cut(s) 374, 654, 1166
FspBI CTAG 5 cut(s) 854, 936, 999, 1176, 1407
GluI GCNGC 3 cut(s) 374, 654, 1166
GsaI CCCAGC 3 cut(s) 653, 796, 1239
GsuI CTGGAG 1 cut(s) 827
HaeIII GGCC 1 cut(s) 943
Hin1II CATG 7 cut(s) 98, 139, 269, 866, 1193, 1291, 1310
HincII GTYRAC 2 cut(s) 767, 1354
HindII GTYRAC 2 cut(s) 767, 1354
HinfI GANTC 3 cut(s) 53, 422, 446
HphI GGTGA 4 cut(s) 16, 476, 851, 1065
Hpy166II GTNNAC 5 cut(s) 185, 505, 572, 767, 1354
Hpy188I TCNGA 7 cut(s) 121, 429, 438, 609, 930, 1299, 1326
Hpy188III TCNNGA 3 cut(s) 101, 332, 671
Hpy8I GTNNAC 5 cut(s) 185, 505, 572, 767, 1354
HpyCH4III ACNGT 5 cut(s) 733, 774, 1056, 1321, 1343
HpyCH4IV ACGT 1 cut(s) 64
HpyCH4V TGCA 8 cut(s) 68, 185, 319, 373, 443, 656, 704, 1193
HpyF10VI GCNNNNNNNGC 2 cut(s) 213, 440
HpyF3I CTNAG 6 cut(s) 308, 366, 606, 630, 780, 927
HpySE526I ACGT 1 cut(s) 64
Hsp92II CATG 7 cut(s) 98, 139, 269, 866, 1193, 1291, 1310
KpnI GGTACC 3 cut(s) 737, 1033, 1095
Kzo9I GATC 5 cut(s) 409, 615, 1070, 1219, 1223
LmnI GCTCC 4 cut(s) 475, 550, 567, 682
Lsp1109I GCAGC 3 cut(s) 385, 640, 1152
LweI GCATC 3 cut(s) 94, 598, 751
MaeI CTAG 5 cut(s) 854, 936, 999, 1176, 1407
MaeII ACGT 1 cut(s) 64
MaeIII GTNAC 4 cut(s) 714, 857, 1154, 1279
MalI GATC 5 cut(s) 411, 617, 1072, 1221, 1225
MboI GATC 5 cut(s) 409, 615, 1070, 1219, 1223
MboII GAAGA 5 cut(s) 151, 394, 881, 902, 1110
MfeI CAATTG 2 cut(s) 416, 695
MflI RGATCY 2 cut(s) 409, 615
MhlI GDGCHC 1 cut(s) 187
MluCI AATT 8 cut(s) 37, 154, 226, 416, 582, 695, 749, 1009
MmeI TCCRAC 1 cut(s) 995
MseI TTAA 2 cut(s) 828, 1047
MslI CAYNNNNRTG 2 cut(s) 270, 1065
MspA1I CMGCKG 3 cut(s) 653, 1165, 1235
MunI CAATTG 2 cut(s) 416, 695
Mva1269I GAATGC 2 cut(s) 436, 445
MwoI GCNNNNNNNGC 2 cut(s) 213, 440
NdeII GATC 5 cut(s) 409, 615, 1070, 1219, 1223
NlaIII CATG 7 cut(s) 98, 139, 269, 866, 1193, 1291, 1310
NlaIV GGNNCC 4 cut(s) 735, 1031, 1093, 1254
NmuCI GTSAC 3 cut(s) 714, 857, 1279
NspI RCATGY 2 cut(s) 98, 1193
NspV TTCGAA 1 cut(s) 60
PciI ACATGT 1 cut(s) 94
PctI GAATGC 2 cut(s) 436, 445
PfeI GAWTC 3 cut(s) 53, 422, 446
PkrI GCNGC 3 cut(s) 375, 655, 1167
PscI ACATGT 1 cut(s) 94
PshAI GACNNNNGTC 1 cut(s) 1341
PspEI GGTNACC 2 cut(s) 857, 1154
PspFI CCCAGC 3 cut(s) 649, 792, 1235
PspN4I GGNNCC 4 cut(s) 735, 1031, 1093, 1254
PspPI GGNCC 2 cut(s) 88, 941
PstI CTGCAG 1 cut(s) 706
PstNI CAGNNNCTG 1 cut(s) 449
PsuI RGATCY 2 cut(s) 409, 615
PvuII CAGCTG 3 cut(s) 653, 1165, 1235
RsaI GTAC 4 cut(s) 602, 735, 1031, 1093
RsaNI GTAC 4 cut(s) 601, 734, 1030, 1092
RseI CAYNNNNRTG 2 cut(s) 270, 1065
SaqAI TTAA 2 cut(s) 828, 1047
SatI GCNGC 3 cut(s) 374, 654, 1166
Sau3AI GATC 5 cut(s) 409, 615, 1070, 1219, 1223
Sau96I GGNCC 2 cut(s) 88, 941
SbfI CCTGCAGG 1 cut(s) 706
ScaI AGTACT 1 cut(s) 602
SdaI CCTGCAGG 1 cut(s) 706
SduI GDGCHC 1 cut(s) 187
SfaNI GCATC 3 cut(s) 94, 598, 751
SfcI CTRYAG 1 cut(s) 702
SfuI TTCGAA 1 cut(s) 60
SinI GGWCC 1 cut(s) 88
SmiMI CAYNNNNRTG 2 cut(s) 270, 1065
SmlI CTYRAG 3 cut(s) 200, 221, 669
SmoI CTYRAG 3 cut(s) 200, 221, 669
Sse8387I CCTGCAGG 1 cut(s) 706
Sse9I AATT 8 cut(s) 37, 154, 226, 416, 582, 695, 749, 1009
SsiI CCGC 4 cut(s) 214, 740, 788, 1203
SspI AATATT 3 cut(s) 304, 644, 962
SspMI CTAG 5 cut(s) 854, 936, 999, 1176, 1407
StyI CCWWGG 2 cut(s) 402, 853
TaaI ACNGT 5 cut(s) 733, 774, 1056, 1321, 1343
TaiI ACGT 1 cut(s) 67
TaqI TCGA 3 cut(s) 60, 1100, 1222
TaqII GACCGA 1 cut(s) 105
TasI AATT 8 cut(s) 37, 154, 226, 416, 582, 695, 749, 1009
TatI WGTACW 1 cut(s) 600
TfiI GAWTC 3 cut(s) 53, 422, 446
Tru1I TTAA 2 cut(s) 828, 1047
Tru9I TTAA 2 cut(s) 828, 1047
TscAI CASTG 1 cut(s) 1200
TseFI GTSAC 3 cut(s) 714, 857, 1279
TseI GCWGC 3 cut(s) 373, 653, 1165
Tsp45I GTSAC 3 cut(s) 714, 857, 1279
TspDTI ATGAA 5 cut(s) 152, 1002, 1065, 1118, 1397
TspRI CASTG 1 cut(s) 1200
VneI GTGCAC 1 cut(s) 183
VpaK11BI GGWCC 1 cut(s) 88
XagI CCTNNNNNAGG 2 cut(s) 852, 1011
XapI RAATTY 1 cut(s) 226
XceI RCATGY 2 cut(s) 98, 1193
XcmI CCANNNNNNNNNTGG 1 cut(s) 1410
XmaJI CCTAGG 1 cut(s) 853
XmiI GTMKAC 1 cut(s) 571
XspI CTAG 5 cut(s) 854, 936, 999, 1176, 1407
ZrmI AGTACT 1 cut(s) 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.