AT3G05070

cwf18 pre-mRNA splicing factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005457
Physical Location & Seq
Forward (+)
1416075 .. 1417842
1768 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G05070.1

Sequence Viewer

Length: 435 bp
ATGGCTAGTGAAGATGAATCGATTGAACAAAAAGCTGCTGCTCGTAAAGAGGCACTTAAAGCTCTAAGGGCAGCTCAGGAACTGTCTGAGACCAAGGAAGACGGAGAGGATGAAGCTGTCGAAGAAGATGGTCCAGCCATGAAGTTTAGAAACTATGTTCCCCAAGCTAAGGAGCTTCAGGATGGTAAACTAGCGCCACCAGAGCTACCCAAATTCGAAGATCCGATCGTTGCACTTCCACCTGCTGTAGAGAAGAAAGAGGACCCATTTGTTAACATCGCTCCAAAGAAGCCAAACTGGGACCTCCGAAGAGATGTGCAGAAGAAGCTTGATAAGCTCGAAAGGCGGACTCAAAAGGCAATGCATAAACTCATGGAGGAGCAAGAGAAAGAACGTGAGATGGCTGAAGCTGATGGTAACACCATAGAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
cwf18

Protein Analysis

144

Amino Acids

16.4

Weight (kDa)

4.87

Isoelectric Point (pI)

71.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cwf18 PF08315 7 - 127 9.4e-34 cwf18 pre-mRNA splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 250
Acc36I ACCTGC 1 cut(s) 250
AciI CCGC 1 cut(s) 346
AclWI GGATC 1 cut(s) 215
AcsI RAATTY 1 cut(s) 212
AcuI CTGAAG 2 cut(s) 161, 426
AfiI CCNNNNNNNGG 1 cut(s) 169
AgsI TTSAA 1 cut(s) 26
Alw26I GTCTC 1 cut(s) 83
AlwI GGATC 1 cut(s) 215
AlwNI CAGNNNCTG 1 cut(s) 82
ApeKI GCWGC 3 cut(s) 35, 38, 71
ApoI RAATTY 1 cut(s) 212
AspLEI GCGC 1 cut(s) 196
AspS9I GGNCC 3 cut(s) 131, 262, 301
AsuII TTCGAA 1 cut(s) 216
AvaII GGWCC 3 cut(s) 131, 262, 301
BbsI GAAGAC 1 cut(s) 105
BbvI GCAGC 3 cut(s) 22, 25, 83
BccI CCATC 4 cut(s) 122, 176, 394, 407
BcoDI GTCTC 1 cut(s) 83
BfaI CTAG 2 cut(s) 6, 191
BfmI CTRYAG 1 cut(s) 246
BfoI RGCGCY 1 cut(s) 197
BfuAI ACCTGC 1 cut(s) 250
BisI GCNGC 3 cut(s) 36, 39, 72
BlsI GCNGC 3 cut(s) 37, 40, 73
Bme18I GGWCC 3 cut(s) 131, 262, 301
BmgT120I GGNCC 3 cut(s) 131, 262, 301
BmiI GGNNCC 2 cut(s) 264, 302
BmrI ACTGGG 1 cut(s) 307
BmuI ACTGGG 1 cut(s) 307
BpiI GAAGAC 1 cut(s) 105
Bpu10I CCTNAGC 2 cut(s) 75, 168
Bpu14I TTCGAA 1 cut(s) 216
Bsa29I ATCGAT 1 cut(s) 20
BsaI GGTCTC 1 cut(s) 83
BsaJI CCNNGG 1 cut(s) 93
Bsc4I CCNNNNNNNGG 1 cut(s) 169
Bse1I ACTGG 1 cut(s) 302
Bse3DI GCAATG 1 cut(s) 366
BseCI ATCGAT 1 cut(s) 20
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 2 cut(s) 115, 187
BseLI CCNNNNNNNGG 1 cut(s) 169
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 2 cut(s) 78, 89
BseNI ACTGG 1 cut(s) 302
BseRI GAGGAG 1 cut(s) 392
BseXI GCAGC 3 cut(s) 22, 25, 83
BsgI GTGCAG 1 cut(s) 338
Bsh1285I CGRYCG 1 cut(s) 228
BshVI ATCGAT 1 cut(s) 20
BsiEI CGRYCG 1 cut(s) 228
BslFI GGGAC 1 cut(s) 314
BslI CCNNNNNNNGG 1 cut(s) 169
BsmAI GTCTC 1 cut(s) 83
BsmFI GGGAC 1 cut(s) 314
Bso31I GGTCTC 1 cut(s) 83
Bsp119I TTCGAA 1 cut(s) 216
Bsp143I GATC 2 cut(s) 220, 225
BspACI CCGC 1 cut(s) 346
BspCNI CTCAG 2 cut(s) 79, 88
BspDI ATCGAT 1 cut(s) 20
BspLI GGNNCC 2 cut(s) 264, 302
BspMI ACCTGC 1 cut(s) 250
BspPI GGATC 1 cut(s) 215
BspT104I TTCGAA 1 cut(s) 216
BspTNI GGTCTC 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 366
BsrI ACTGG 1 cut(s) 302
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 2 cut(s) 220, 225
BssT1I CCWWGG 1 cut(s) 93
Bst4CI ACNGT 1 cut(s) 84
Bst6I CTCTTC 1 cut(s) 304
BstBI TTCGAA 1 cut(s) 216
BstDEI CTNAG 4 cut(s) 65, 75, 87, 168
BstF5I GGATG 2 cut(s) 115, 187
BstH2I RGCGCY 1 cut(s) 197
BstHHI GCGC 1 cut(s) 196
BstKTI GATC 2 cut(s) 223, 228
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 2 cut(s) 220, 225
BstMCI CGRYCG 1 cut(s) 228
BstMWI GCNNNNNNNGC 6 cut(s) 59, 68, 202, 325, 334, 343
BstSFI CTRYAG 1 cut(s) 246
BstV1I GCAGC 3 cut(s) 22, 25, 83
BstV2I GAAGAC 1 cut(s) 105
BstX2I RGATCY 1 cut(s) 220
BstYI RGATCY 1 cut(s) 220
Bsu15I ATCGAT 1 cut(s) 20
BsuTUI ATCGAT 1 cut(s) 20
BtgZI GCGATG 1 cut(s) 262
BtsCI GGATG 2 cut(s) 115, 187
BveI ACCTGC 1 cut(s) 250
CaiI CAGNNNCTG 1 cut(s) 82
CfoI GCGC 1 cut(s) 196
Cfr13I GGNCC 3 cut(s) 131, 262, 301
ClaI ATCGAT 1 cut(s) 20
CviAII CATG 2 cut(s) 139, 373
DdeI CTNAG 4 cut(s) 65, 75, 87, 168
DpnI GATC 2 cut(s) 222, 227
DpnII GATC 2 cut(s) 220, 225
Eam1104I CTCTTC 1 cut(s) 304
EarI CTCTTC 1 cut(s) 304
EciI GGCGGA 1 cut(s) 361
Eco130I CCWWGG 1 cut(s) 93
Eco31I GGTCTC 1 cut(s) 83
Eco47I GGWCC 3 cut(s) 131, 262, 301
Eco57I CTGAAG 2 cut(s) 161, 426
EcoO109I RGGNCCY 2 cut(s) 262, 301
EcoT14I CCWWGG 1 cut(s) 93
EcoT22I ATGCAT 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 2 cut(s) 142, 376
FaiI YATR 5 cut(s) 140, 156, 366, 374, 425
FalI AAGNNNNNCTT 2 cut(s) 39, 71
FaqI GGGAC 1 cut(s) 314
FatI CATG 2 cut(s) 138, 372
Fnu4HI GCNGC 3 cut(s) 36, 39, 72
FokI GGATG 2 cut(s) 122, 194
Fsp4HI GCNGC 3 cut(s) 36, 39, 72
FspBI CTAG 2 cut(s) 6, 191
GlaI GCGC 1 cut(s) 195
GluI GCNGC 3 cut(s) 36, 39, 72
HaeII RGCGCY 1 cut(s) 197
HhaI GCGC 1 cut(s) 196
Hin1II CATG 2 cut(s) 142, 376
Hin6I GCGC 1 cut(s) 194
HinP1I GCGC 1 cut(s) 194
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 2 cut(s) 17, 349
HpaI GTTAAC 1 cut(s) 274
Hpy166II GTNNAC 2 cut(s) 188, 274
Hpy188I TCNGA 3 cut(s) 88, 225, 308
Hpy188III TCNNGA 2 cut(s) 77, 179
Hpy8I GTNNAC 2 cut(s) 188, 274
HpyCH4III ACNGT 1 cut(s) 84
HpyCH4IV ACGT 1 cut(s) 394
HpyCH4V TGCA 3 cut(s) 233, 319, 364
HpyF10VI GCNNNNNNNGC 6 cut(s) 59, 68, 202, 325, 334, 343
HpyF3I CTNAG 4 cut(s) 65, 75, 87, 168
HpySE526I ACGT 1 cut(s) 394
Hsp92II CATG 2 cut(s) 142, 376
HspAI GCGC 1 cut(s) 194
KspAI GTTAAC 1 cut(s) 274
Kzo9I GATC 2 cut(s) 220, 225
LmnI GCTCC 3 cut(s) 172, 286, 379
LpnPI CCDG 6 cut(s) 62, 147, 164, 213, 255, 283
Lsp1109I GCAGC 3 cut(s) 22, 25, 83
MaeI CTAG 2 cut(s) 6, 191
MaeII ACGT 1 cut(s) 394
MaeIII GTNAC 1 cut(s) 416
MalI GATC 2 cut(s) 222, 227
MboI GATC 2 cut(s) 220, 225
MboII GAAGA 8 cut(s) 23, 110, 134, 137, 230, 265, 321, 334
MflI RGATCY 1 cut(s) 220
MluCI AATT 1 cut(s) 212
MlyI GAGTC 1 cut(s) 343
MnlI CCTC 5 cut(s) 43, 100, 253, 314, 370
Mph1103I ATGCAT 1 cut(s) 366
MseI TTAA 2 cut(s) 57, 273
MwoI GCNNNNNNNGC 6 cut(s) 59, 68, 202, 325, 334, 343
NdeII GATC 2 cut(s) 220, 225
NlaIII CATG 2 cut(s) 142, 376
NlaIV GGNNCC 2 cut(s) 264, 302
NsiI ATGCAT 1 cut(s) 366
NspV TTCGAA 1 cut(s) 216
PaqCI CACCTGC 1 cut(s) 250
PfeI GAWTC 1 cut(s) 17
PkrI GCNGC 3 cut(s) 37, 40, 73
Ple19I CGATCG 1 cut(s) 228
PleI GAGTC 1 cut(s) 343
PpsI GAGTC 1 cut(s) 343
PpuMI RGGWCCY 2 cut(s) 262, 301
Psp5II RGGWCCY 2 cut(s) 262, 301
PspN4I GGNNCC 2 cut(s) 264, 302
PspPI GGNCC 3 cut(s) 131, 262, 301
PspPPI RGGWCCY 2 cut(s) 262, 301
PstNI CAGNNNCTG 1 cut(s) 82
PsuI RGATCY 1 cut(s) 220
PvuI CGATCG 1 cut(s) 228
SaqAI TTAA 2 cut(s) 57, 273
SatI GCNGC 3 cut(s) 36, 39, 72
Sau3AI GATC 2 cut(s) 220, 225
Sau96I GGNCC 3 cut(s) 131, 262, 301
SchI GAGTC 1 cut(s) 343
SfcI CTRYAG 1 cut(s) 246
SfuI TTCGAA 1 cut(s) 216
SinI GGWCC 3 cut(s) 131, 262, 301
Sse9I AATT 1 cut(s) 212
SsiI CCGC 1 cut(s) 346
SspMI CTAG 2 cut(s) 6, 191
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 1 cut(s) 84
TaiI ACGT 1 cut(s) 397
TaqI TCGA 4 cut(s) 20, 120, 216, 339
TasI AATT 1 cut(s) 212
TfiI GAWTC 1 cut(s) 17
Tru1I TTAA 2 cut(s) 57, 273
Tru9I TTAA 2 cut(s) 57, 273
TseI GCWGC 3 cut(s) 35, 38, 71
TspDTI ATGAA 3 cut(s) 30, 126, 155
TspGWI ACGGA 1 cut(s) 117
VpaK11BI GGWCC 3 cut(s) 131, 262, 301
XapI RAATTY 1 cut(s) 212
XspI CTAG 2 cut(s) 6, 191
Zsp2I ATGCAT 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.