FvH4_5g18550

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005457
Physical Location & Seq
Reverse (-)
10674932 .. 10677141
2210 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g18550.t1

Sequence Viewer

Length: 489 bp
ATGGCTGGCGAAGAGGAGTCCATGGAAGAAATCGTTGCGGCACGAAAAGAGAGGCTAAGAGCCCTCAAAGCTGCCCAGGAGCTATTAAACACTCCTGATGAGGACTCTGCTCAACTTGAAAGCAACACCAACAATGAGCAGGAGGATAAGAGAGACGAAGCTCCCGACAATGATGTCGATGAAAATAGTACCAACATGAAGTTCCGAAACTATGTTCCTCATGATAAGAAGCTTCAGGAAGCGAAGCTAGAGCCACCGAAGCCATCTAAGTTTGAAGAGCCTGTGGCGGCAGTGGCTCCTCCATCTGAGAAGACAGAGGACCCGTTCGTGAATATTGCTCCGAAAAAGCCAAACTGGGAACTTCGTAGGGATGTGCAGAAGAAGCTTGATAAGCTTGAAAGGCGAACACAGAAGGCAATGTGTAAACTTATGGAACAAGAAAAGCAGAAGCAATTGGAAGAAGAAGGTATCAATGGTGCTGCGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
cwf18

Protein Analysis

163

Amino Acids

18.59

Weight (kDa)

4.87

Isoelectric Point (pI)

59.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cwf18 PF08315 7 - 146 2.3e-30 cwf18 pre-mRNA splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 173
AciI CCGC 3 cut(s) 38, 287, 482
AcuI CTGAAG 1 cut(s) 218
AfaI GTAC 1 cut(s) 190
AgsI TTSAA 3 cut(s) 119, 275, 398
AjnI CCWGG 1 cut(s) 75
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AluBI AGCT 7 cut(s) 71, 82, 161, 232, 247, 385, 394
AluI AGCT 7 cut(s) 71, 82, 161, 232, 247, 385, 394
Alw26I GTCTC 1 cut(s) 147
ApeKI GCWGC 2 cut(s) 71, 479
AspS9I GGNCC 1 cut(s) 319
AvaII GGWCC 1 cut(s) 319
BanII GRGCYC 1 cut(s) 64
BbsI GAAGAC 1 cut(s) 317
BbvI GCAGC 2 cut(s) 58, 466
BccI CCATC 2 cut(s) 271, 310
BciT130I CCWGG 1 cut(s) 77
BcoDI GTCTC 1 cut(s) 147
BfaI CTAG 1 cut(s) 248
BisI GCNGC 4 cut(s) 39, 72, 288, 480
BlsI GCNGC 4 cut(s) 40, 73, 289, 481
Bme1390I CCNGG 1 cut(s) 77
Bme18I GGWCC 1 cut(s) 319
BmgT120I GGNCC 1 cut(s) 319
BmiI GGNNCC 2 cut(s) 297, 321
BmrFI CCNGG 1 cut(s) 77
BmrI ACTGGG 1 cut(s) 364
BmuI ACTGGG 1 cut(s) 364
BpiI GAAGAC 1 cut(s) 317
BsaJI CCNNGG 2 cut(s) 21, 75
Bse1I ACTGG 1 cut(s) 359
Bse3DI GCAATG 1 cut(s) 423
BseBI CCWGG 1 cut(s) 77
BseDI CCNNGG 2 cut(s) 21, 75
BseGI GGATG 1 cut(s) 376
BseMI GCAATG 1 cut(s) 423
BseMII CTCAG 1 cut(s) 297
BseNI ACTGG 1 cut(s) 359
BseRI GAGGAG 2 cut(s) 29, 288
BseXI GCAGC 2 cut(s) 58, 466
BsgI GTGCAG 1 cut(s) 395
BsmAI GTCTC 1 cut(s) 147
BsmBI CGTCTC 1 cut(s) 147
Bsp1286I GDGCHC 1 cut(s) 64
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 3 cut(s) 38, 287, 482
BspCNI CTCAG 1 cut(s) 298
BspHI TCATGA 1 cut(s) 220
BspLI GGNNCC 2 cut(s) 297, 321
BspQI GCTCTTC 1 cut(s) 270
BsrDI GCAATG 1 cut(s) 423
BsrI ACTGG 1 cut(s) 359
BssECI CCNNGG 2 cut(s) 21, 75
BssT1I CCWWGG 1 cut(s) 21
Bst2UI CCWGG 1 cut(s) 77
Bst6I CTCTTC 2 cut(s) 6, 270
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 3 cut(s) 56, 267, 306
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 1 cut(s) 376
BstMAI GTCTC 1 cut(s) 147
BstMWI GCNNNNNNNGC 6 cut(s) 68, 259, 293, 382, 391, 400
BstNI CCWGG 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 75
BstV1I GCAGC 2 cut(s) 58, 466
BstV2I GAAGAC 1 cut(s) 317
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 1 cut(s) 376
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 1 cut(s) 297
Cac8I GCNNGC 1 cut(s) 7
CciI TCATGA 1 cut(s) 220
Cfr13I GGNCC 1 cut(s) 319
Csp6I GTAC 1 cut(s) 189
CviAII CATG 3 cut(s) 22, 196, 221
CviQI GTAC 1 cut(s) 189
DdeI CTNAG 3 cut(s) 56, 267, 306
DrdI GACNNNNNNGTC 1 cut(s) 173
DseDI GACNNNNNNGTC 1 cut(s) 173
Eam1104I CTCTTC 2 cut(s) 6, 270
EarI CTCTTC 2 cut(s) 6, 270
Eco130I CCWWGG 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 64
Eco47I GGWCC 1 cut(s) 319
Eco57I CTGAAG 1 cut(s) 218
EcoO109I RGGNCCY 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 75
EcoT14I CCWWGG 1 cut(s) 21
EcoT38I GRGCYC 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 21
Esp3I CGTCTC 1 cut(s) 147
FaeI CATG 3 cut(s) 25, 199, 224
FaiI YATR 5 cut(s) 23, 197, 213, 222, 431
FatI CATG 3 cut(s) 21, 195, 220
Fnu4HI GCNGC 4 cut(s) 39, 72, 288, 480
FokI GGATG 1 cut(s) 383
FriOI GRGCYC 1 cut(s) 64
Fsp4HI GCNGC 4 cut(s) 39, 72, 288, 480
FspBI CTAG 1 cut(s) 248
GluI GCNGC 4 cut(s) 39, 72, 288, 480
Hin1II CATG 3 cut(s) 25, 199, 224
HindIII AAGCTT 3 cut(s) 230, 383, 392
HinfI GANTC 2 cut(s) 17, 104
Hpy166II GTNNAC 1 cut(s) 425
Hpy188I TCNGA 3 cut(s) 206, 307, 342
Hpy188III TCNNGA 5 cut(s) 95, 164, 221, 236, 328
Hpy8I GTNNAC 1 cut(s) 425
HpyAV CCTTC 2 cut(s) 406, 458
HpyCH4V TGCA 1 cut(s) 376
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 259, 293, 382, 391, 400
HpyF3I CTNAG 3 cut(s) 56, 267, 306
Hsp92II CATG 3 cut(s) 25, 199, 224
LguI GCTCTTC 1 cut(s) 270
LmnI GCTCC 4 cut(s) 79, 166, 301, 343
LpnPI CCDG 7 cut(s) 62, 89, 108, 125, 221, 294, 340
Lsp1109I GCAGC 2 cut(s) 58, 466
MaeI CTAG 1 cut(s) 248
MboII GAAGA 7 cut(s) 23, 38, 287, 322, 391, 470, 473
MfeI CAATTG 1 cut(s) 452
MhlI GDGCHC 1 cut(s) 64
MluCI AATT 1 cut(s) 452
MlyI GAGTC 2 cut(s) 26, 98
MnlI CCTC 8 cut(s) 7, 45, 74, 94, 136, 228, 309, 310
MseI TTAA 1 cut(s) 86
MspR9I CCNGG 1 cut(s) 77
MunI CAATTG 1 cut(s) 452
MvaI CCWGG 1 cut(s) 77
MwoI GCNNNNNNNGC 6 cut(s) 68, 259, 293, 382, 391, 400
NcoI CCATGG 1 cut(s) 21
NlaIII CATG 3 cut(s) 25, 199, 224
NlaIV GGNNCC 2 cut(s) 297, 321
PagI TCATGA 1 cut(s) 220
PciSI GCTCTTC 1 cut(s) 270
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PkrI GCNGC 4 cut(s) 40, 73, 289, 481
PleI GAGTC 2 cut(s) 25, 98
PpsI GAGTC 2 cut(s) 25, 98
PpuMI RGGWCCY 1 cut(s) 319
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 1 cut(s) 75
PspGI CCWGG 1 cut(s) 75
PspN4I GGNNCC 2 cut(s) 297, 321
PspPI GGNCC 1 cut(s) 319
PspPPI RGGWCCY 1 cut(s) 319
RsaI GTAC 1 cut(s) 190
RsaNI GTAC 1 cut(s) 189
SapI GCTCTTC 1 cut(s) 270
SaqAI TTAA 1 cut(s) 86
SatI GCNGC 4 cut(s) 39, 72, 288, 480
Sau96I GGNCC 1 cut(s) 319
SchI GAGTC 2 cut(s) 26, 98
ScrFI CCNGG 1 cut(s) 77
SduI GDGCHC 1 cut(s) 64
SetI ASST 8 cut(s) 73, 84, 163, 234, 249, 387, 396, 469
SinI GGWCC 1 cut(s) 319
Sse9I AATT 1 cut(s) 452
SsiI CCGC 3 cut(s) 38, 287, 482
SspI AATATT 1 cut(s) 334
SspMI CTAG 1 cut(s) 248
StyD4I CCNGG 1 cut(s) 75
StyI CCWWGG 1 cut(s) 21
TaqI TCGA 1 cut(s) 177
TasI AATT 1 cut(s) 452
TauI GCSGC 2 cut(s) 41, 290
Tru1I TTAA 1 cut(s) 86
Tru9I TTAA 1 cut(s) 86
TscAI CASTG 1 cut(s) 297
TseI GCWGC 2 cut(s) 71, 479
TspDTI ATGAA 2 cut(s) 195, 212
TspRI CASTG 1 cut(s) 297
VpaK11BI GGWCC 1 cut(s) 319
XspI CTAG 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.