Rh7AG001800

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0005457
Physical Location & Seq
Forward (+)
188008 .. 190223
2216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG001800.1

Sequence Viewer

Length: 561 bp
ATGAAGCATACGTCGTCCTGCCCTAATTTGGTGTCTCCAGTCTCGCCTCCTCCTGCTGCTGCTAACAGTCTGCGATTTAGGCAAATGGCTGCTGAAGAGGACTCCCTCGAAGAAATCGTTGCGGCACGAAAGGAGAGGCTCAGAGCCCTCAAAGCTGCACAGCAACTGTTAAACACTCCGGATGAGGACTCTGCTCAACTTGAAAGTAACACTAACGATGACCAGGAAAAGAGCAATGAAACTCCTGATGGGGATGAAACTACCAACATGAAATTCCGAAATTATGTTCCTCATGATAAGAAGCTTCAGGAAGGGAAGCTAGATCCACCAAAACCATCCAAGTTTGAAGACCCTGTTGCAGCAGTGCCTCCTCCATCAGAGAAGAAAGAGGACCCATTCGTGAATATTGCTCCTAAAAAACCAAACTGGGAACTTCGTAGGGATGTGCAGAAGAAGCTTGATAAGCTTGAAAGGCGAACCCAGAAGGCAATGTGTAAACTTATGGAACAAGAAAAGCAGAGGCAACTGGATGAAGACAGCATCAATGGTGCAGGGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
cwf18

Protein Analysis

186

Amino Acids

21.03

Weight (kDa)

5.44

Isoelectric Point (pI)

59.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cwf18 PF08315 35 - 170 1.5e-31 cwf18 pre-mRNA splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 178
AciI CCGC 1 cut(s) 122
AclWI GGATC 1 cut(s) 317
AcsI RAATTY 1 cut(s) 272
AcuI CTGAAG 2 cut(s) 114, 290
AfiI CCNNNNNNNGG 1 cut(s) 28
AgsI TTSAA 3 cut(s) 203, 347, 470
AjnI CCWGG 1 cut(s) 222
AjuI GAANNNNNNNTTGG 2 cut(s) 257, 289
AluBI AGCT 5 cut(s) 155, 304, 319, 457, 466
AluI AGCT 5 cut(s) 155, 304, 319, 457, 466
Alw26I GTCTC 2 cut(s) 39, 46
AlwI GGATC 1 cut(s) 317
AlwNI CAGNNNCTG 1 cut(s) 166
Aor13HI TCCGGA 1 cut(s) 178
ApeKI GCWGC 5 cut(s) 56, 59, 89, 155, 359
ApoI RAATTY 1 cut(s) 272
AspS9I GGNCC 1 cut(s) 391
AvaII GGWCC 1 cut(s) 391
BanII GRGCYC 1 cut(s) 148
BbsI GAAGAC 2 cut(s) 354, 540
BbvI GCAGC 5 cut(s) 43, 46, 76, 142, 371
BccI CCATC 3 cut(s) 242, 343, 382
BciT130I CCWGG 1 cut(s) 224
BcoDI GTCTC 2 cut(s) 39, 46
BfaI CTAG 1 cut(s) 320
BisI GCNGC 6 cut(s) 57, 60, 90, 123, 156, 360
BlsI GCNGC 6 cut(s) 58, 61, 91, 124, 157, 361
Bme1390I CCNGG 1 cut(s) 224
Bme18I GGWCC 1 cut(s) 391
BmgT120I GGNCC 1 cut(s) 391
BmiI GGNNCC 1 cut(s) 393
BmrFI CCNGG 1 cut(s) 224
BmrI ACTGGG 1 cut(s) 436
BmsI GCATC 1 cut(s) 549
BmuI ACTGGG 1 cut(s) 436
BpiI GAAGAC 2 cut(s) 354, 540
BpmI CTGGAG 1 cut(s) 21
BsaWI WCCGGW 1 cut(s) 178
Bsc4I CCNNNNNNNGG 1 cut(s) 28
Bse1I ACTGG 3 cut(s) 38, 431, 531
Bse3DI GCAATG 2 cut(s) 241, 495
BseAI TCCGGA 1 cut(s) 178
BseBI CCWGG 1 cut(s) 224
BseGI GGATG 5 cut(s) 187, 259, 335, 448, 535
BseLI CCNNNNNNNGG 1 cut(s) 28
BseMI GCAATG 2 cut(s) 241, 495
BseMII CTCAG 1 cut(s) 154
BseNI ACTGG 3 cut(s) 38, 431, 531
BseRI GAGGAG 2 cut(s) 39, 360
BseXI GCAGC 5 cut(s) 43, 46, 76, 142, 371
BsgI GTGCAG 2 cut(s) 141, 467
BsiSI CCGG 1 cut(s) 179
BslI CCNNNNNNNGG 1 cut(s) 28
BsmAI GTCTC 2 cut(s) 39, 46
Bsp1286I GDGCHC 1 cut(s) 148
Bsp13I TCCGGA 1 cut(s) 178
Bsp143I GATC 1 cut(s) 322
BspACI CCGC 1 cut(s) 122
BspCNI CTCAG 1 cut(s) 153
BspEI TCCGGA 1 cut(s) 178
BspHI TCATGA 1 cut(s) 292
BspLI GGNNCC 1 cut(s) 393
BspPI GGATC 1 cut(s) 317
BsrDI GCAATG 2 cut(s) 241, 495
BsrI ACTGG 3 cut(s) 38, 431, 531
BssMI GATC 1 cut(s) 322
Bst2UI CCWGG 1 cut(s) 224
Bst4CI ACNGT 2 cut(s) 68, 168
Bst6I CTCTTC 1 cut(s) 90
BstDEI CTNAG 1 cut(s) 140
BstF5I GGATG 5 cut(s) 187, 259, 335, 448, 535
BstKTI GATC 1 cut(s) 325
BstMAI GTCTC 2 cut(s) 39, 46
BstMBI GATC 1 cut(s) 322
BstMWI GCNNNNNNNGC 5 cut(s) 79, 152, 454, 463, 472
BstNI CCWGG 1 cut(s) 224
BstSCI CCNGG 1 cut(s) 222
BstV1I GCAGC 5 cut(s) 43, 46, 76, 142, 371
BstV2I GAAGAC 2 cut(s) 354, 540
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
BtsCI GGATG 5 cut(s) 187, 259, 335, 448, 535
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 1 cut(s) 369
CaiI CAGNNNCTG 1 cut(s) 166
CciI TCATGA 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 391
CviAII CATG 2 cut(s) 268, 293
CviJI RGCY 8 cut(s) 89, 139, 146, 155, 304, 319, 457, 466
CviKI_1 RGCY 8 cut(s) 89, 139, 146, 155, 304, 319, 457, 466
DdeI CTNAG 1 cut(s) 140
DpnI GATC 1 cut(s) 324
DpnII GATC 1 cut(s) 322
Eam1104I CTCTTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 391
Eco57I CTGAAG 2 cut(s) 114, 290
EcoO109I RGGNCCY 1 cut(s) 391
EcoRII CCWGG 1 cut(s) 222
EcoT38I GRGCYC 1 cut(s) 148
FaeI CATG 2 cut(s) 271, 296
FaiI YATR 5 cut(s) 9, 269, 285, 294, 503
FatI CATG 2 cut(s) 267, 292
Fnu4HI GCNGC 6 cut(s) 57, 60, 90, 123, 156, 360
FokI GGATG 5 cut(s) 194, 266, 322, 455, 542
FriOI GRGCYC 1 cut(s) 148
Fsp4HI GCNGC 6 cut(s) 57, 60, 90, 123, 156, 360
FspBI CTAG 1 cut(s) 320
GluI GCNGC 6 cut(s) 57, 60, 90, 123, 156, 360
GsuI CTGGAG 1 cut(s) 21
HapII CCGG 1 cut(s) 179
Hin1II CATG 2 cut(s) 271, 296
HindIII AAGCTT 3 cut(s) 302, 455, 464
HinfI GANTC 2 cut(s) 101, 188
HpaII CCGG 1 cut(s) 179
Hpy166II GTNNAC 1 cut(s) 497
Hpy188I TCNGA 3 cut(s) 143, 278, 379
Hpy188III TCNNGA 5 cut(s) 179, 245, 293, 308, 400
Hpy8I GTNNAC 1 cut(s) 497
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 2 cut(s) 305, 478
HpyCH4III ACNGT 2 cut(s) 68, 168
HpyCH4IV ACGT 1 cut(s) 11
HpyCH4V TGCA 4 cut(s) 158, 359, 448, 551
HpyF10VI GCNNNNNNNGC 5 cut(s) 79, 152, 454, 463, 472
HpyF3I CTNAG 1 cut(s) 140
HpySE526I ACGT 1 cut(s) 11
Hsp92II CATG 2 cut(s) 271, 296
Kpn2I TCCGGA 1 cut(s) 178
Kzo9I GATC 1 cut(s) 322
LmnI GCTCC 1 cut(s) 415
Lsp1109I GCAGC 5 cut(s) 43, 46, 76, 142, 371
LweI GCATC 1 cut(s) 549
MaeI CTAG 1 cut(s) 320
MaeII ACGT 1 cut(s) 11
MaeIII GTNAC 1 cut(s) 206
MalI GATC 1 cut(s) 324
MboI GATC 1 cut(s) 322
MboII GAAGA 6 cut(s) 107, 122, 359, 394, 463, 545
MflI RGATCY 1 cut(s) 322
MhlI GDGCHC 1 cut(s) 148
MluCI AATT 3 cut(s) 25, 272, 280
MlyI GAGTC 2 cut(s) 95, 182
MroI TCCGGA 1 cut(s) 178
MseI TTAA 1 cut(s) 170
MspI CCGG 1 cut(s) 179
MspR9I CCNGG 1 cut(s) 224
MvaI CCWGG 1 cut(s) 224
MwoI GCNNNNNNNGC 5 cut(s) 79, 152, 454, 463, 472
NdeII GATC 1 cut(s) 322
NlaIII CATG 2 cut(s) 271, 296
NlaIV GGNNCC 1 cut(s) 393
PagI TCATGA 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PkrI GCNGC 6 cut(s) 58, 61, 91, 124, 157, 361
PleI GAGTC 2 cut(s) 95, 182
PpsI GAGTC 2 cut(s) 95, 182
PpuMI RGGWCCY 1 cut(s) 391
Psp5II RGGWCCY 1 cut(s) 391
Psp6I CCWGG 1 cut(s) 222
PspGI CCWGG 1 cut(s) 222
PspN4I GGNNCC 1 cut(s) 393
PspPI GGNCC 1 cut(s) 391
PspPPI RGGWCCY 1 cut(s) 391
PstNI CAGNNNCTG 1 cut(s) 166
PsuI RGATCY 1 cut(s) 322
SaqAI TTAA 1 cut(s) 170
SatI GCNGC 6 cut(s) 57, 60, 90, 123, 156, 360
Sau3AI GATC 1 cut(s) 322
Sau96I GGNCC 1 cut(s) 391
SchI GAGTC 2 cut(s) 95, 182
ScrFI CCNGG 1 cut(s) 224
SduI GDGCHC 1 cut(s) 148
SetI ASST 6 cut(s) 14, 157, 306, 321, 459, 468
SfaNI GCATC 1 cut(s) 549
SinI GGWCC 1 cut(s) 391
Sse9I AATT 3 cut(s) 25, 272, 280
SsiI CCGC 1 cut(s) 122
SspI AATATT 1 cut(s) 406
SspMI CTAG 1 cut(s) 320
StyD4I CCNGG 1 cut(s) 222
TaaI ACNGT 2 cut(s) 68, 168
TaiI ACGT 1 cut(s) 14
TaqI TCGA 1 cut(s) 108
TasI AATT 3 cut(s) 25, 272, 280
TauI GCSGC 1 cut(s) 125
Tru1I TTAA 1 cut(s) 170
Tru9I TTAA 1 cut(s) 170
TscAI CASTG 1 cut(s) 369
TseI GCWGC 5 cut(s) 56, 59, 89, 155, 359
TspDTI ATGAA 5 cut(s) 17, 252, 270, 284, 546
TspRI CASTG 1 cut(s) 369
VpaK11BI GGWCC 1 cut(s) 391
XapI RAATTY 1 cut(s) 272
XspI CTAG 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.