RLG00000031374

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0005457
Physical Location & Seq
Forward (+)
4484283 .. 4484744
462 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031374

Sequence Viewer

Length: 462 bp
ATGGCAGGTCAAGAGGACTCCATTGAAGTAGTTGCTGCGACACGAAAAGAGATGATAAGAACCCTTAGAGCTGCACAGGAACTGTCAAACACTCCGGATGAGGACTCTGCTTGTATTAACAATAACACTGACGATGACAACGAAGCTAGTGAAGAAACTAATATGAGTGTGAAGTTCCGAAATTATTTCCCTCGTGATAAGAAGCTTATGGAAGGAAAGCTTAAAACTGGTCCAATATTGAAGTTTGATGACCCTATTGCAGCAGTAGTGCCTTTTCCATCAGAGAAGAAAAAGGACCCGTTCAAGGATCTTGTTCCCAAACCACCACACTTTGAACTTCAAAGGGATGTGCAGAAGAGGCTTGATAAGCTTGAAAGGCGAACGCAGAAGGCGTTGTGTAAAATTATGGCGCAAGAAAGGCTGAAGCAAGAGAATGAAGGTGGCATAAATGGTCCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
cwf18

Protein Analysis

154

Amino Acids

17.4

Weight (kDa)

5.98

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cwf18 PF08315 8 - 138 2.1e-18 cwf18 pre-mRNA splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 94
AclWI GGATC 1 cut(s) 315
AcuI CTGAAG 1 cut(s) 443
AfiI CCNNNNNNNGG 1 cut(s) 304
AgsI TTSAA 6 cut(s) 26, 241, 304, 335, 341, 374
AluBI AGCT 6 cut(s) 71, 146, 205, 220, 370, 459
AluI AGCT 6 cut(s) 71, 146, 205, 220, 370, 459
AlwI GGATC 1 cut(s) 315
AlwNI CAGNNNCTG 1 cut(s) 82
Aor13HI TCCGGA 1 cut(s) 94
ApeKI GCWGC 3 cut(s) 35, 71, 260
AspLEI GCGC 1 cut(s) 412
AspS9I GGNCC 3 cut(s) 230, 295, 452
AvaII GGWCC 3 cut(s) 230, 295, 452
BauI CACGAG 1 cut(s) 192
BbvI GCAGC 3 cut(s) 22, 58, 272
BccI CCATC 1 cut(s) 286
BfaI CTAG 2 cut(s) 147, 460
BisI GCNGC 3 cut(s) 36, 72, 261
BlsI GCNGC 3 cut(s) 37, 73, 262
Bme18I GGWCC 3 cut(s) 230, 295, 452
BmgT120I GGNCC 3 cut(s) 230, 295, 452
BmiI GGNNCC 1 cut(s) 297
BsaWI WCCGGW 1 cut(s) 94
Bsc4I CCNNNNNNNGG 1 cut(s) 304
Bse1I ACTGG 1 cut(s) 232
BseAI TCCGGA 1 cut(s) 94
BseGI GGATG 2 cut(s) 103, 352
BseLI CCNNNNNNNGG 1 cut(s) 304
BseNI ACTGG 1 cut(s) 232
BseXI GCAGC 3 cut(s) 22, 58, 272
BsgI GTGCAG 2 cut(s) 57, 371
BsiSI CCGG 1 cut(s) 95
BslI CCNNNNNNNGG 1 cut(s) 304
Bsp13I TCCGGA 1 cut(s) 94
Bsp143I GATC 1 cut(s) 307
BspEI TCCGGA 1 cut(s) 94
BspLI GGNNCC 1 cut(s) 297
BspPI GGATC 1 cut(s) 315
BsrI ACTGG 1 cut(s) 232
BssMI GATC 1 cut(s) 307
BssSI CACGAG 1 cut(s) 192
Bst2BI CACGAG 1 cut(s) 192
Bst4CI ACNGT 1 cut(s) 84
Bst6I CTCTTC 1 cut(s) 350
BstDEI CTNAG 1 cut(s) 65
BstF5I GGATG 2 cut(s) 103, 352
BstHHI GCGC 1 cut(s) 412
BstKTI GATC 1 cut(s) 310
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 4 cut(s) 358, 367, 376, 418
BstV1I GCAGC 3 cut(s) 22, 58, 272
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BtsCI GGATG 2 cut(s) 103, 352
BtsIMutI CAGTG 1 cut(s) 126
CaiI CAGNNNCTG 1 cut(s) 82
CfoI GCGC 1 cut(s) 412
Cfr13I GGNCC 3 cut(s) 230, 295, 452
CviJI RGCY 8 cut(s) 71, 146, 205, 220, 361, 370, 421, 459
CviKI_1 RGCY 8 cut(s) 71, 146, 205, 220, 361, 370, 421, 459
DdeI CTNAG 1 cut(s) 65
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
Eco47I GGWCC 3 cut(s) 230, 295, 452
Eco57I CTGAAG 1 cut(s) 443
EcoO109I RGGNCCY 1 cut(s) 295
FaiI YATR 4 cut(s) 164, 209, 407, 446
FalI AAGNNNNNCTT 2 cut(s) 204, 236
Fnu4HI GCNGC 3 cut(s) 36, 72, 261
FokI GGATG 2 cut(s) 110, 359
Fsp4HI GCNGC 3 cut(s) 36, 72, 261
FspBI CTAG 2 cut(s) 147, 460
GlaI GCGC 1 cut(s) 411
GluI GCNGC 3 cut(s) 36, 72, 261
HapII CCGG 1 cut(s) 95
HhaI GCGC 1 cut(s) 412
Hin6I GCGC 1 cut(s) 410
HinP1I GCGC 1 cut(s) 410
HindIII AAGCTT 3 cut(s) 203, 218, 368
HinfI GANTC 2 cut(s) 17, 104
HpaII CCGG 1 cut(s) 95
Hpy188I TCNGA 2 cut(s) 179, 283
Hpy188III TCNNGA 3 cut(s) 11, 95, 194
HpyAV CCTTC 3 cut(s) 206, 382, 431
HpyCH4III ACNGT 1 cut(s) 84
HpyCH4V TGCA 3 cut(s) 74, 260, 352
HpyF10VI GCNNNNNNNGC 4 cut(s) 358, 367, 376, 418
HpyF3I CTNAG 1 cut(s) 65
HspAI GCGC 1 cut(s) 410
Kpn2I TCCGGA 1 cut(s) 94
Kzo9I GATC 1 cut(s) 307
LpnPI CCDG 3 cut(s) 62, 108, 213
Lsp1109I GCAGC 3 cut(s) 22, 58, 272
MaeI CTAG 2 cut(s) 147, 460
MalI GATC 1 cut(s) 309
MboI GATC 1 cut(s) 307
MboII GAAGA 3 cut(s) 164, 298, 367
MflI RGATCY 1 cut(s) 307
MluCI AATT 2 cut(s) 181, 402
MlyI GAGTC 2 cut(s) 11, 98
MnlI CCTC 4 cut(s) 7, 94, 201, 351
MroI TCCGGA 1 cut(s) 94
MseI TTAA 2 cut(s) 117, 222
MspI CCGG 1 cut(s) 95
MwoI GCNNNNNNNGC 4 cut(s) 358, 367, 376, 418
NdeII GATC 1 cut(s) 307
NlaIV GGNNCC 1 cut(s) 297
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 389
PkrI GCNGC 3 cut(s) 37, 73, 262
PleI GAGTC 2 cut(s) 11, 98
PpsI GAGTC 2 cut(s) 11, 98
PpuMI RGGWCCY 1 cut(s) 295
Psp5II RGGWCCY 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 297
PspPI GGNCC 3 cut(s) 230, 295, 452
PspPPI RGGWCCY 1 cut(s) 295
PstNI CAGNNNCTG 1 cut(s) 82
PsuI RGATCY 1 cut(s) 307
SaqAI TTAA 2 cut(s) 117, 222
SatI GCNGC 3 cut(s) 36, 72, 261
Sau3AI GATC 1 cut(s) 307
Sau96I GGNCC 3 cut(s) 230, 295, 452
SchI GAGTC 2 cut(s) 11, 98
SetI ASST 8 cut(s) 10, 73, 148, 207, 222, 372, 442, 461
SinI GGWCC 3 cut(s) 230, 295, 452
Sse9I AATT 2 cut(s) 181, 402
SspI AATATT 1 cut(s) 237
SspMI CTAG 2 cut(s) 147, 460
TaaI ACNGT 1 cut(s) 84
TasI AATT 2 cut(s) 181, 402
Tru1I TTAA 2 cut(s) 117, 222
Tru9I TTAA 2 cut(s) 117, 222
TscAI CASTG 1 cut(s) 133
TseI GCWGC 3 cut(s) 35, 71, 260
TspDTI ATGAA 1 cut(s) 450
TspRI CASTG 1 cut(s) 133
VpaK11BI GGWCC 3 cut(s) 230, 295, 452
XspI CTAG 2 cut(s) 147, 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.