RchiOBHm_Chr5g0006211

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0005457
Physical Location & Seq
Forward (+)
3878180 .. 3878641
462 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28735

Sequence Viewer

Length: 462 bp
ATGGCAGGTCAAGAGGACTCCATTGAAGTAGTTGCTGCGACACGAAAAGAGATGATAAGAGCCCTTAGAGCTGCACAGGAACTGTCAAACACTCCGGATGAGGACTCTGCTCATATTAACAATAACACTGACGATGACAACGACGCTAGTGAAGAAACTAATATGAGTGTGAAGTTCCGAAATTATTTCCCTCGTGATAAGAAGCTTATGGAAGGAAAGCTTAAAATTGGTCCAATATTGAAGTTTGATGACCCTATTGCTGCAGTAGTGCCTTTTCCATCAGAGAAGAAAAAGGACCCGTTCAAGGATCTTGTTCCCAAACCACCACACTTTGAACTTCAAAGGGATGTGCAGAAGAGGCTTGATAAGCTTGAAAGGCGAACGCAGAAGGCGTTGTGTAAAATTATGGAGCAAGAAAGGCTGAAGCAAGAGAATGAAGGTGGCATAAATGGTCTAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
cwf18

Protein Analysis

153

Amino Acids

17.48

Weight (kDa)

5.81

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cwf18 PF08315 8 - 138 1.2e-18 cwf18 pre-mRNA splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 94
AclWI GGATC 1 cut(s) 315
AcuI CTGAAG 1 cut(s) 443
AfiI CCNNNNNNNGG 1 cut(s) 304
AgsI TTSAA 6 cut(s) 26, 241, 304, 335, 341, 374
AluBI AGCT 5 cut(s) 71, 205, 220, 370, 459
AluI AGCT 5 cut(s) 71, 205, 220, 370, 459
AlwI GGATC 1 cut(s) 315
AlwNI CAGNNNCTG 1 cut(s) 82
Aor13HI TCCGGA 1 cut(s) 94
ApeKI GCWGC 3 cut(s) 35, 71, 260
AspS9I GGNCC 2 cut(s) 230, 295
AvaII GGWCC 2 cut(s) 230, 295
BanII GRGCYC 1 cut(s) 64
BauI CACGAG 1 cut(s) 192
BbvI GCAGC 3 cut(s) 22, 58, 247
BccI CCATC 1 cut(s) 286
BfaI CTAG 2 cut(s) 147, 460
BfmI CTRYAG 1 cut(s) 261
BisI GCNGC 3 cut(s) 36, 72, 261
BlsI GCNGC 3 cut(s) 37, 73, 262
Bme18I GGWCC 2 cut(s) 230, 295
BmgT120I GGNCC 2 cut(s) 230, 295
BmiI GGNNCC 1 cut(s) 297
BsaWI WCCGGW 1 cut(s) 94
Bsc4I CCNNNNNNNGG 1 cut(s) 304
BseAI TCCGGA 1 cut(s) 94
BseGI GGATG 2 cut(s) 103, 352
BseLI CCNNNNNNNGG 1 cut(s) 304
BseXI GCAGC 3 cut(s) 22, 58, 247
BsgI GTGCAG 2 cut(s) 57, 371
BsiSI CCGG 1 cut(s) 95
BslI CCNNNNNNNGG 1 cut(s) 304
Bsp1286I GDGCHC 1 cut(s) 64
Bsp13I TCCGGA 1 cut(s) 94
Bsp143I GATC 1 cut(s) 307
BspEI TCCGGA 1 cut(s) 94
BspLI GGNNCC 1 cut(s) 297
BspMAI CTGCAG 1 cut(s) 265
BspPI GGATC 1 cut(s) 315
BssMI GATC 1 cut(s) 307
BssSI CACGAG 1 cut(s) 192
Bst2BI CACGAG 1 cut(s) 192
Bst4CI ACNGT 1 cut(s) 84
Bst6I CTCTTC 1 cut(s) 350
BstDEI CTNAG 2 cut(s) 65, 455
BstF5I GGATG 2 cut(s) 103, 352
BstKTI GATC 1 cut(s) 310
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 5 cut(s) 68, 358, 367, 376, 418
BstSFI CTRYAG 1 cut(s) 261
BstV1I GCAGC 3 cut(s) 22, 58, 247
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BtsCI GGATG 2 cut(s) 103, 352
BtsIMutI CAGTG 1 cut(s) 126
CaiI CAGNNNCTG 1 cut(s) 82
Cfr13I GGNCC 2 cut(s) 230, 295
CseI GACGC 1 cut(s) 152
CviJI RGCY 8 cut(s) 62, 71, 205, 220, 361, 370, 421, 459
CviKI_1 RGCY 8 cut(s) 62, 71, 205, 220, 361, 370, 421, 459
DdeI CTNAG 2 cut(s) 65, 455
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
Eco24I GRGCYC 1 cut(s) 64
Eco47I GGWCC 2 cut(s) 230, 295
Eco57I CTGAAG 1 cut(s) 443
EcoO109I RGGNCCY 1 cut(s) 295
EcoT38I GRGCYC 1 cut(s) 64
FaiI YATR 5 cut(s) 114, 164, 209, 407, 446
FalI AAGNNNNNCTT 2 cut(s) 204, 236
Fnu4HI GCNGC 3 cut(s) 36, 72, 261
FokI GGATG 2 cut(s) 110, 359
FriOI GRGCYC 1 cut(s) 64
Fsp4HI GCNGC 3 cut(s) 36, 72, 261
FspBI CTAG 2 cut(s) 147, 460
GluI GCNGC 3 cut(s) 36, 72, 261
HapII CCGG 1 cut(s) 95
HgaI GACGC 1 cut(s) 152
HindIII AAGCTT 3 cut(s) 203, 218, 368
HinfI GANTC 2 cut(s) 17, 104
HpaII CCGG 1 cut(s) 95
Hpy188I TCNGA 2 cut(s) 179, 283
Hpy188III TCNNGA 3 cut(s) 11, 95, 194
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 3 cut(s) 206, 382, 431
HpyCH4III ACNGT 1 cut(s) 84
HpyCH4V TGCA 3 cut(s) 74, 263, 352
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 358, 367, 376, 418
HpyF3I CTNAG 2 cut(s) 65, 455
Kpn2I TCCGGA 1 cut(s) 94
Kzo9I GATC 1 cut(s) 307
LmnI GCTCC 1 cut(s) 409
LpnPI CCDG 2 cut(s) 62, 108
Lsp1109I GCAGC 3 cut(s) 22, 58, 247
MaeI CTAG 2 cut(s) 147, 460
MalI GATC 1 cut(s) 309
MboI GATC 1 cut(s) 307
MboII GAAGA 3 cut(s) 164, 298, 367
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 1 cut(s) 64
MluCI AATT 3 cut(s) 181, 225, 402
MlyI GAGTC 2 cut(s) 11, 98
MnlI CCTC 4 cut(s) 7, 94, 201, 351
MroI TCCGGA 1 cut(s) 94
MseI TTAA 2 cut(s) 117, 222
MspI CCGG 1 cut(s) 95
MwoI GCNNNNNNNGC 5 cut(s) 68, 358, 367, 376, 418
NdeII GATC 1 cut(s) 307
NlaIV GGNNCC 1 cut(s) 297
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 389
PkrI GCNGC 3 cut(s) 37, 73, 262
PleI GAGTC 2 cut(s) 11, 98
PpsI GAGTC 2 cut(s) 11, 98
PpuMI RGGWCCY 1 cut(s) 295
Psp5II RGGWCCY 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 297
PspPI GGNCC 2 cut(s) 230, 295
PspPPI RGGWCCY 1 cut(s) 295
PstI CTGCAG 1 cut(s) 265
PstNI CAGNNNCTG 1 cut(s) 82
PsuI RGATCY 1 cut(s) 307
SaqAI TTAA 2 cut(s) 117, 222
SatI GCNGC 3 cut(s) 36, 72, 261
Sau3AI GATC 1 cut(s) 307
Sau96I GGNCC 2 cut(s) 230, 295
SchI GAGTC 2 cut(s) 11, 98
SduI GDGCHC 1 cut(s) 64
SetI ASST 7 cut(s) 10, 73, 207, 222, 372, 442, 461
SfcI CTRYAG 1 cut(s) 261
SinI GGWCC 2 cut(s) 230, 295
Sse9I AATT 3 cut(s) 181, 225, 402
SspI AATATT 1 cut(s) 237
SspMI CTAG 2 cut(s) 147, 460
TaaI ACNGT 1 cut(s) 84
TasI AATT 3 cut(s) 181, 225, 402
Tru1I TTAA 2 cut(s) 117, 222
Tru9I TTAA 2 cut(s) 117, 222
TscAI CASTG 1 cut(s) 133
TseI GCWGC 3 cut(s) 35, 71, 260
TspDTI ATGAA 1 cut(s) 450
TspRI CASTG 1 cut(s) 133
VpaK11BI GGWCC 2 cut(s) 230, 295
XspI CTAG 2 cut(s) 147, 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.