Rw5G004800

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005457
Physical Location & Seq
Reverse (-)
5012536 .. 5012985
450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G004800.1

Sequence Viewer

Length: 450 bp
ATGGCAGGTCAAGAGGACTCCATTGAAGTTGCTGCGACACGAAAAGAGATGATAAGAGCCCTTAGAGCTGCACAGGAACTGTCAAACACTCCGGATGAGGACTCTGCTCGTATTAACAATAACACTGACGATGACAACGAAGCTAGTGAAGAAACTAATATGAGTGTGAAGTTCCGAAATTATTTCCCTCGTGATAAGAAGCTTATGGAAGGAAAGCTTAAAACTGGTCCAATATTGAAGTTTGATGACCCTATTGCAGCAGTGGTGTCTTTTCCATCAGAGAAGAAGTTCAAGGATCTTGTTCCCAAACCACCACACTTTGAACTTCAAAGGGATGTGCAGAAGAGGCTTGATAAGCTTGAAAGGCGAACGCAGAAGGCGTTGTGTAAAATTATGGAGCAAGAAAGGCTGAGGCAAGAGAATGAAGGTGGCATAAATGGTCCAAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
cwf18

Protein Analysis

149

Amino Acids

17.06

Weight (kDa)

5.99

Isoelectric Point (pI)

43.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cwf18 PF08315 10 - 134 3.1e-16 cwf18 pre-mRNA splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 91
AclWI GGATC 1 cut(s) 303
AgsI TTSAA 6 cut(s) 26, 238, 292, 323, 329, 362
AluBI AGCT 6 cut(s) 68, 143, 202, 217, 358, 447
AluI AGCT 6 cut(s) 68, 143, 202, 217, 358, 447
AlwI GGATC 1 cut(s) 303
AlwNI CAGNNNCTG 1 cut(s) 79
Aor13HI TCCGGA 1 cut(s) 91
ApeKI GCWGC 3 cut(s) 32, 68, 257
Asp700I GAANNNNTTC 1 cut(s) 287
AspS9I GGNCC 2 cut(s) 227, 440
AvaII GGWCC 2 cut(s) 227, 440
BanII GRGCYC 1 cut(s) 61
BauI CACGAG 1 cut(s) 189
BbvCI CCTCAGC 1 cut(s) 410
BbvI GCAGC 3 cut(s) 19, 55, 269
BccI CCATC 1 cut(s) 283
BfaI CTAG 2 cut(s) 144, 448
BisI GCNGC 3 cut(s) 33, 69, 258
BlsI GCNGC 3 cut(s) 34, 70, 259
Bme18I GGWCC 2 cut(s) 227, 440
BmgT120I GGNCC 2 cut(s) 227, 440
Bpu10I CCTNAGC 1 cut(s) 410
BsaWI WCCGGW 1 cut(s) 91
Bse1I ACTGG 1 cut(s) 229
BseAI TCCGGA 1 cut(s) 91
BseGI GGATG 2 cut(s) 100, 340
BseMII CTCAG 1 cut(s) 401
BseNI ACTGG 1 cut(s) 229
BseXI GCAGC 3 cut(s) 19, 55, 269
BsgI GTGCAG 2 cut(s) 54, 359
BsiSI CCGG 1 cut(s) 92
Bsp1286I GDGCHC 1 cut(s) 61
Bsp13I TCCGGA 1 cut(s) 91
Bsp143I GATC 1 cut(s) 295
BspCNI CTCAG 1 cut(s) 402
BspEI TCCGGA 1 cut(s) 91
BspPI GGATC 1 cut(s) 303
BsrI ACTGG 1 cut(s) 229
BssMI GATC 1 cut(s) 295
BssSI CACGAG 1 cut(s) 189
Bst2BI CACGAG 1 cut(s) 189
Bst4CI ACNGT 1 cut(s) 81
Bst6I CTCTTC 1 cut(s) 338
BstDEI CTNAG 2 cut(s) 62, 410
BstF5I GGATG 2 cut(s) 100, 340
BstKTI GATC 1 cut(s) 298
BstMBI GATC 1 cut(s) 295
BstMWI GCNNNNNNNGC 5 cut(s) 65, 346, 355, 364, 406
BstV1I GCAGC 3 cut(s) 19, 55, 269
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BtsCI GGATG 2 cut(s) 100, 340
BtsI GCAGTG 1 cut(s) 267
BtsIMutI CAGTG 2 cut(s) 123, 267
CaiI CAGNNNCTG 1 cut(s) 79
Cfr13I GGNCC 2 cut(s) 227, 440
CviJI RGCY 9 cut(s) 59, 68, 143, 202, 217, 349, 358, 409, 447
CviKI_1 RGCY 9 cut(s) 59, 68, 143, 202, 217, 349, 358, 409, 447
DdeI CTNAG 2 cut(s) 62, 410
DpnI GATC 1 cut(s) 297
DpnII GATC 1 cut(s) 295
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
Eco24I GRGCYC 1 cut(s) 61
Eco47I GGWCC 2 cut(s) 227, 440
EcoT38I GRGCYC 1 cut(s) 61
FaiI YATR 4 cut(s) 161, 206, 395, 434
FalI AAGNNNNNCTT 2 cut(s) 201, 233
Fnu4HI GCNGC 3 cut(s) 33, 69, 258
FokI GGATG 2 cut(s) 107, 347
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 3 cut(s) 33, 69, 258
FspBI CTAG 2 cut(s) 144, 448
GluI GCNGC 3 cut(s) 33, 69, 258
HapII CCGG 1 cut(s) 92
HindIII AAGCTT 3 cut(s) 200, 215, 356
HinfI GANTC 2 cut(s) 17, 101
HpaII CCGG 1 cut(s) 92
Hpy188I TCNGA 2 cut(s) 176, 280
Hpy188III TCNNGA 3 cut(s) 11, 92, 191
HpyAV CCTTC 3 cut(s) 203, 370, 419
HpyCH4III ACNGT 1 cut(s) 81
HpyCH4V TGCA 3 cut(s) 71, 257, 340
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 346, 355, 364, 406
HpyF3I CTNAG 2 cut(s) 62, 410
Kpn2I TCCGGA 1 cut(s) 91
Kzo9I GATC 1 cut(s) 295
LmnI GCTCC 1 cut(s) 397
LpnPI CCDG 3 cut(s) 59, 105, 210
Lsp1109I GCAGC 3 cut(s) 19, 55, 269
MaeI CTAG 2 cut(s) 144, 448
MalI GATC 1 cut(s) 297
MboI GATC 1 cut(s) 295
MboII GAAGA 3 cut(s) 161, 295, 355
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 1 cut(s) 61
MluCI AATT 2 cut(s) 178, 390
MlyI GAGTC 2 cut(s) 11, 95
MnlI CCTC 5 cut(s) 7, 91, 198, 339, 405
MroI TCCGGA 1 cut(s) 91
MroXI GAANNNNTTC 1 cut(s) 287
MseI TTAA 2 cut(s) 114, 219
MspI CCGG 1 cut(s) 92
MwoI GCNNNNNNNGC 5 cut(s) 65, 346, 355, 364, 406
NdeII GATC 1 cut(s) 295
PcsI WCGNNNNNNNCGW 2 cut(s) 135, 377
PdmI GAANNNNTTC 1 cut(s) 287
PkrI GCNGC 3 cut(s) 34, 70, 259
PleI GAGTC 2 cut(s) 11, 95
PpsI GAGTC 2 cut(s) 11, 95
PspPI GGNCC 2 cut(s) 227, 440
PstNI CAGNNNCTG 1 cut(s) 79
PsuI RGATCY 1 cut(s) 295
SaqAI TTAA 2 cut(s) 114, 219
SatI GCNGC 3 cut(s) 33, 69, 258
Sau3AI GATC 1 cut(s) 295
Sau96I GGNCC 2 cut(s) 227, 440
SchI GAGTC 2 cut(s) 11, 95
SduI GDGCHC 1 cut(s) 61
SetI ASST 8 cut(s) 10, 70, 145, 204, 219, 360, 430, 449
SinI GGWCC 2 cut(s) 227, 440
Sse9I AATT 2 cut(s) 178, 390
SspI AATATT 1 cut(s) 234
SspMI CTAG 2 cut(s) 144, 448
TaaI ACNGT 1 cut(s) 81
TasI AATT 2 cut(s) 178, 390
Tru1I TTAA 2 cut(s) 114, 219
Tru9I TTAA 2 cut(s) 114, 219
TscAI CASTG 2 cut(s) 130, 267
TseI GCWGC 3 cut(s) 32, 68, 257
TspDTI ATGAA 1 cut(s) 438
TspRI CASTG 2 cut(s) 130, 267
VpaK11BI GGWCC 2 cut(s) 227, 440
XmnI GAANNNNTTC 1 cut(s) 287
XspI CTAG 2 cut(s) 144, 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.