FvH4_3g03920

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005457
Physical Location & Seq
Forward (+)
2183855 .. 2184805
951 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g03920.t1

Sequence Viewer

Length: 444 bp
ATGGCTGGTCAAGAGGCCTCCGTTGAAGAAGTTGCTGCAACACGAAGAGAGATGCTAAGAGCCCTTAGAGCTGCACAGAAGCTGTCAAACTCTCCGGATGAAGACTCTTCTTCTGTTGACAATAACAATGTCGATGACATCGACGAAGTTAGTGAAGGAACGAATACGAGTGTCAAGTTCCGAAATTATTTCCCTCGTGATAAGAAGCTTATTAAGGAACAAGAAGGGAAGCTTGAATCTGGTCCAACATCCAAGTTTGATGATCCTATTGGAGCAGTGGTGCCTTCTCCATTAGAGAAGAAAAAGGACTCGTTCAAGGATCTTGTTCCCAGACCACCACACTTTGAACTTCAAAGGGATGTGCAGAAGAGGCTGGATAAGCTTGAAAGGCGAACGCTGAAGGCGTTATGTAAAATTATGGAGCAAGAGAATGAAGGTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
cwf18

Protein Analysis

148

Amino Acids

16.6

Weight (kDa)

5.39

Isoelectric Point (pI)

44.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cwf18 PF08315 7 - 142 1.5e-17 cwf18 pre-mRNA splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 280
AccIII TCCGGA 1 cut(s) 94
AclWI GGATC 2 cut(s) 257, 327
AcuI CTGAAG 1 cut(s) 419
AgsI TTSAA 6 cut(s) 26, 236, 316, 347, 353, 386
AluBI AGCT 5 cut(s) 71, 82, 208, 232, 382
AluI AGCT 5 cut(s) 71, 82, 208, 232, 382
AlwI GGATC 2 cut(s) 257, 327
AlwNI CAGNNNCTG 1 cut(s) 82
Aor13HI TCCGGA 1 cut(s) 94
AoxI GGCC 1 cut(s) 15
ApeKI GCWGC 2 cut(s) 35, 71
AspS9I GGNCC 1 cut(s) 242
AvaII GGWCC 1 cut(s) 242
BanI GGYRCC 1 cut(s) 280
BanII GRGCYC 1 cut(s) 64
BauI CACGAG 1 cut(s) 195
BbsI GAAGAC 1 cut(s) 108
BbvI GCAGC 2 cut(s) 22, 58
BisI GCNGC 2 cut(s) 36, 72
BlsI GCNGC 2 cut(s) 37, 73
Bme18I GGWCC 1 cut(s) 242
BmgT120I GGNCC 1 cut(s) 242
BmiI GGNNCC 1 cut(s) 282
BmsI GCATC 1 cut(s) 42
BpiI GAAGAC 1 cut(s) 108
BsaWI WCCGGW 1 cut(s) 94
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
BseAI TCCGGA 1 cut(s) 94
BseGI GGATG 3 cut(s) 103, 248, 364
BseXI GCAGC 2 cut(s) 22, 58
BsgI GTGCAG 2 cut(s) 57, 383
BshFI GGCC 1 cut(s) 17
BshNI GGYRCC 1 cut(s) 280
BsiSI CCGG 1 cut(s) 95
BsnI GGCC 1 cut(s) 17
Bsp1286I GDGCHC 1 cut(s) 64
Bsp13I TCCGGA 1 cut(s) 94
Bsp143I GATC 2 cut(s) 262, 319
BspANI GGCC 1 cut(s) 17
BspEI TCCGGA 1 cut(s) 94
BspLI GGNNCC 1 cut(s) 282
BspPI GGATC 2 cut(s) 257, 327
BspT107I GGYRCC 1 cut(s) 280
BssMI GATC 2 cut(s) 262, 319
BssSI CACGAG 1 cut(s) 195
Bst2BI CACGAG 1 cut(s) 195
Bst6I CTCTTC 3 cut(s) 40, 112, 362
BstDEI CTNAG 2 cut(s) 56, 65
BstF5I GGATG 3 cut(s) 103, 248, 364
BstKTI GATC 2 cut(s) 265, 322
BstMBI GATC 2 cut(s) 262, 319
BstMWI GCNNNNNNNGC 4 cut(s) 68, 370, 379, 388
BstV1I GCAGC 2 cut(s) 22, 58
BstV2I GAAGAC 1 cut(s) 108
BstX2I RGATCY 1 cut(s) 319
BstYI RGATCY 1 cut(s) 319
BsuRI GGCC 1 cut(s) 17
BtsCI GGATG 3 cut(s) 103, 248, 364
BtsI GCAGTG 1 cut(s) 282
BtsIMutI CAGTG 1 cut(s) 282
CaiI CAGNNNCTG 1 cut(s) 82
Cfr13I GGNCC 1 cut(s) 242
CviJI RGCY 9 cut(s) 5, 17, 62, 71, 82, 208, 232, 373, 382
CviKI_1 RGCY 9 cut(s) 5, 17, 62, 71, 82, 208, 232, 373, 382
DdeI CTNAG 2 cut(s) 56, 65
DpnI GATC 2 cut(s) 264, 321
DpnII GATC 2 cut(s) 262, 319
Eam1104I CTCTTC 3 cut(s) 40, 112, 362
EarI CTCTTC 3 cut(s) 40, 112, 362
Eco147I AGGCCT 1 cut(s) 17
Eco24I GRGCYC 1 cut(s) 64
Eco47I GGWCC 1 cut(s) 242
Eco57I CTGAAG 1 cut(s) 419
EcoT38I GRGCYC 1 cut(s) 64
FaiI YATR 3 cut(s) 409, 419, 442
FalI AAGNNNNNCTT 2 cut(s) 216, 248
Fnu4HI GCNGC 2 cut(s) 36, 72
FokI GGATG 3 cut(s) 110, 235, 371
FriOI GRGCYC 1 cut(s) 64
Fsp4HI GCNGC 2 cut(s) 36, 72
GluI GCNGC 2 cut(s) 36, 72
HaeIII GGCC 1 cut(s) 17
HapII CCGG 1 cut(s) 95
HincII GTYRAC 1 cut(s) 118
HindII GTYRAC 1 cut(s) 118
HindIII AAGCTT 3 cut(s) 206, 230, 380
HinfI GANTC 3 cut(s) 104, 236, 308
HpaII CCGG 1 cut(s) 95
Hpy166II GTNNAC 1 cut(s) 118
Hpy188I TCNGA 1 cut(s) 182
Hpy188III TCNNGA 3 cut(s) 11, 95, 197
Hpy8I GTNNAC 1 cut(s) 118
Hpy99I CGWCG 1 cut(s) 146
HpyAV CCTTC 5 cut(s) 149, 218, 294, 394, 428
HpyCH4V TGCA 4 cut(s) 38, 74, 364, 440
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 370, 379, 388
HpyF3I CTNAG 2 cut(s) 56, 65
Kpn2I TCCGGA 1 cut(s) 94
Kzo9I GATC 2 cut(s) 262, 319
LmnI GCTCC 2 cut(s) 272, 421
LpnPI CCDG 4 cut(s) 108, 225, 343, 359
Lsp1109I GCAGC 2 cut(s) 22, 58
LweI GCATC 1 cut(s) 42
MalI GATC 2 cut(s) 264, 321
MboI GATC 2 cut(s) 262, 319
MboII GAAGA 7 cut(s) 38, 57, 99, 102, 113, 310, 379
MflI RGATCY 1 cut(s) 319
MhlI GDGCHC 1 cut(s) 64
MluCI AATT 2 cut(s) 184, 414
MlyI GAGTC 2 cut(s) 98, 302
MmeI TCCRAC 1 cut(s) 269
MnlI CCTC 4 cut(s) 7, 28, 204, 363
MroI TCCGGA 1 cut(s) 94
MseI TTAA 1 cut(s) 213
MspI CCGG 1 cut(s) 95
MwoI GCNNNNNNNGC 4 cut(s) 68, 370, 379, 388
NdeII GATC 2 cut(s) 262, 319
NlaIV GGNNCC 1 cut(s) 282
PceI AGGCCT 1 cut(s) 17
PcsI WCGNNNNNNNCGW 2 cut(s) 138, 401
PfeI GAWTC 1 cut(s) 236
PkrI GCNGC 2 cut(s) 37, 73
PleI GAGTC 2 cut(s) 98, 302
PpsI GAGTC 2 cut(s) 98, 302
PspN4I GGNNCC 1 cut(s) 282
PspPI GGNCC 1 cut(s) 242
PstNI CAGNNNCTG 1 cut(s) 82
PsuI RGATCY 1 cut(s) 319
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 2 cut(s) 36, 72
Sau3AI GATC 2 cut(s) 262, 319
Sau96I GGNCC 1 cut(s) 242
SchI GAGTC 2 cut(s) 98, 302
SduI GDGCHC 1 cut(s) 64
SetI ASST 6 cut(s) 73, 84, 210, 234, 384, 439
SfaNI GCATC 1 cut(s) 42
SinI GGWCC 1 cut(s) 242
Sse9I AATT 2 cut(s) 184, 414
SseBI AGGCCT 1 cut(s) 17
StuI AGGCCT 1 cut(s) 17
TaqI TCGA 2 cut(s) 132, 141
TasI AATT 2 cut(s) 184, 414
TfiI GAWTC 1 cut(s) 236
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 1 cut(s) 282
TseI GCWGC 2 cut(s) 35, 71
TspDTI ATGAA 1 cut(s) 114
TspGWI ACGGA 1 cut(s) 10
TspRI CASTG 1 cut(s) 282
VpaK11BI GGWCC 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.