pycom06g21570

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0005457
Physical Location & Seq
Reverse (-)
24161985 .. 24164614
2630 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g21570.1

Sequence Viewer

Length: 729 bp
ATGCAAATGAGGGAGACAATACTTACAATACATACGGGGCACGCAAGGCAGACCCAACTCGTTTTTAAATACGTGGCTCGCTCAGCAGAAGCCCATTTATCTCACCTGGCCCAACTCATGCTCTATCTTTCTTTTTCCACCTCTTTTGTTTCTCCACCCTCTCAAATTCTCAATTCTTCCATTCCATTCCAAAGTGACAGCGTTAAAAGTGCATTGCAGACGACGGGTCAGGGACCTTCTCTTCGTCTTCTCCGCATAGGCGAAGCCATGGCCGCTGAAGAGGACTCCATTGAACAAGCAGTCGCAGCACGTCAAGAGAGGCTAAGAGCTCTTAAAGCAGCTCAGGAACTGTTAAACACTCCAGATGAGGATTCCGCACAAGTTCACGATAACACTAATGAAAACAATGCAATTAGTGACGAAACTAATACCAACATGAAATTCCGAAACTATGTTCCTCATGATAAGCAGCTTCAGGAGGGAAAGCTTGTTCCACCAGTGCTGCCAAAGTTTGAAGACCCTGTTGCAGGAGTACCACCTCCATCAGAGAAGAAAGAGGACCCGTTTGTGAATATTGCTCCTAAAAAACCAAACTGGGAACTTCGCAGGGATGTGCAGAAGAGGCTTGATAAGCTTGAAAAGCGAACCCAAAAGGCAATGCTTAAACTTATGGAGGAACAGGAGAAGCAAAAGCAATTGGATGGAGATGGCAGAAATGGTGAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
cwf18

Protein Analysis

243

Amino Acids

27.29

Weight (kDa)

6.12

Isoelectric Point (pI)

55.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cwf18 PF08315 96 - 226 5.7e-30 cwf18 pre-mRNA splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 253, 273, 375
AcoI YGGCCR 1 cut(s) 270
AcsI RAATTY 2 cut(s) 165, 440
AcuI CTGAAG 2 cut(s) 297, 458
AfaI GTAC 1 cut(s) 534
AfiI CCNNNNNNNGG 1 cut(s) 527
AgsI TTSAA 3 cut(s) 293, 515, 638
AhdI GACNNNNNGTC 1 cut(s) 225
AjiI CACGTC 1 cut(s) 311
AjnI CCWGG 1 cut(s) 105
AjuI GAANNNNNNNTTGG 2 cut(s) 425, 457
AloI GAACNNNNNNTCC 2 cut(s) 474, 506
AluBI AGCT 5 cut(s) 329, 341, 472, 487, 634
AluI AGCT 5 cut(s) 329, 341, 472, 487, 634
Alw21I GWGCWC 1 cut(s) 331
Alw26I GTCTC 1 cut(s) 8
AlwNI CAGNNNCTG 1 cut(s) 349
AoxI GGCC 2 cut(s) 108, 270
ApeKI GCWGC 4 cut(s) 305, 338, 469, 502
ApoI RAATTY 2 cut(s) 165, 440
ArsI GACNNNNNNTTYG 2 cut(s) 225, 257
AspS9I GGNCC 3 cut(s) 109, 233, 559
AsuHPI GGTGA 1 cut(s) 95
AvaII GGWCC 2 cut(s) 233, 559
BaeGI GKGCMC 1 cut(s) 42
BanII GRGCYC 1 cut(s) 331
BbsI GAAGAC 2 cut(s) 239, 522
Bbv12I GWGCWC 1 cut(s) 331
BbvI GCAGC 4 cut(s) 317, 350, 481, 489
BccI CCATC 3 cut(s) 550, 695, 701
BciT130I CCWGG 1 cut(s) 107
BcoDI GTCTC 1 cut(s) 8
BfaI CTAG 1 cut(s) 727
BisI GCNGC 5 cut(s) 273, 306, 339, 470, 503
BlpI GCTNAGC 1 cut(s) 82
BlsI GCNGC 5 cut(s) 274, 307, 340, 471, 504
Bme1390I CCNGG 1 cut(s) 107
Bme18I GGWCC 2 cut(s) 233, 559
BmeRI GACNNNNNGTC 1 cut(s) 225
BmgBI CACGTC 1 cut(s) 311
BmgT120I GGNCC 3 cut(s) 109, 233, 559
BmiI GGNNCC 2 cut(s) 234, 561
BmrFI CCNGG 1 cut(s) 107
BmrI ACTGGG 1 cut(s) 604
BmuI ACTGGG 1 cut(s) 604
BpiI GAAGAC 2 cut(s) 239, 522
BpmI CTGGAG 1 cut(s) 345
Bpu10I CCTNAGC 1 cut(s) 342
Bpu1102I GCTNAGC 1 cut(s) 82
BsaAI YACGTR 1 cut(s) 73
BsaJI CCNNGG 1 cut(s) 267
Bsc4I CCNNNNNNNGG 1 cut(s) 527
Bse1I ACTGG 2 cut(s) 497, 599
Bse3DI GCAATG 2 cut(s) 212, 663
BseBI CCWGG 1 cut(s) 107
BseDI CCNNGG 1 cut(s) 267
BseGI GGATG 2 cut(s) 616, 706
BseLI CCNNNNNNNGG 1 cut(s) 527
BseMI GCAATG 2 cut(s) 212, 663
BseMII CTCAG 2 cut(s) 96, 356
BseNI ACTGG 2 cut(s) 497, 599
BseSI GKGCMC 1 cut(s) 42
BseXI GCAGC 4 cut(s) 317, 350, 481, 489
BsgI GTGCAG 1 cut(s) 635
BshFI GGCC 2 cut(s) 110, 272
BsiHKAI GWGCWC 1 cut(s) 331
BslFI GGGAC 1 cut(s) 246
BslI CCNNNNNNNGG 1 cut(s) 527
BsmAI GTCTC 1 cut(s) 8
BsmFI GGGAC 1 cut(s) 246
BsnI GGCC 2 cut(s) 110, 272
Bsp1286I GDGCHC 2 cut(s) 42, 331
Bsp1720I GCTNAGC 1 cut(s) 82
Bsp19I CCATGG 1 cut(s) 267
BspACI CCGC 3 cut(s) 253, 273, 375
BspANI GGCC 2 cut(s) 110, 272
BspCNI CTCAG 2 cut(s) 95, 355
BspHI TCATGA 1 cut(s) 460
BspLI GGNNCC 2 cut(s) 234, 561
BsrDI GCAATG 2 cut(s) 212, 663
BsrI ACTGG 2 cut(s) 497, 599
BssECI CCNNGG 1 cut(s) 267
BssT1I CCWWGG 1 cut(s) 267
Bst2UI CCWGG 1 cut(s) 107
Bst4CI ACNGT 1 cut(s) 351
Bst6I CTCTTC 3 cut(s) 246, 273, 614
BstBAI YACGTR 1 cut(s) 73
BstC8I GCNNGC 2 cut(s) 42, 79
BstDEI CTNAG 3 cut(s) 82, 323, 342
BstDSI CCRYGG 1 cut(s) 267
BstENI CCTNNNNNAGG 1 cut(s) 525
BstF5I GGATG 2 cut(s) 616, 706
BstMAI GTCTC 1 cut(s) 8
BstMWI GCNNNNNNNGC 8 cut(s) 46, 83, 272, 305, 335, 622, 631, 640
BstNI CCWGG 1 cut(s) 107
BstSCI CCNGG 1 cut(s) 105
BstSLI GKGCMC 1 cut(s) 42
BstV1I GCAGC 4 cut(s) 317, 350, 481, 489
BstV2I GAAGAC 2 cut(s) 239, 522
BsuRI GGCC 2 cut(s) 110, 272
BtgI CCRYGG 1 cut(s) 267
BtrI CACGTC 1 cut(s) 311
BtsCI GGATG 2 cut(s) 616, 706
BtsIMutI CAGTG 1 cut(s) 504
Cac8I GCNNGC 2 cut(s) 42, 79
CaiI CAGNNNCTG 1 cut(s) 349
CciI TCATGA 1 cut(s) 460
Cfr13I GGNCC 3 cut(s) 109, 233, 559
Csp6I GTAC 1 cut(s) 533
CspCI CAANNNNNGTGG 2 cut(s) 127, 162
CviAII CATG 4 cut(s) 118, 268, 436, 461
CviQI GTAC 1 cut(s) 533
DdeI CTNAG 3 cut(s) 82, 323, 342
DraI TTTAAA 1 cut(s) 67
DriI GACNNNNNGTC 1 cut(s) 225
EaeI YGGCCR 1 cut(s) 270
Eam1104I CTCTTC 3 cut(s) 246, 273, 614
Eam1105I GACNNNNNGTC 1 cut(s) 225
EarI CTCTTC 3 cut(s) 246, 273, 614
Ecl136II GAGCTC 1 cut(s) 329
Eco130I CCWWGG 1 cut(s) 267
Eco24I GRGCYC 1 cut(s) 331
Eco47I GGWCC 2 cut(s) 233, 559
Eco53kI GAGCTC 1 cut(s) 329
Eco57I CTGAAG 2 cut(s) 297, 458
EcoICRI GAGCTC 1 cut(s) 329
EcoNI CCTNNNNNAGG 1 cut(s) 525
EcoO109I RGGNCCY 2 cut(s) 233, 559
EcoRII CCWGG 1 cut(s) 105
EcoT14I CCWWGG 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 331
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 4 cut(s) 121, 271, 439, 464
FaiI YATR 8 cut(s) 33, 119, 257, 269, 437, 453, 462, 671
FaqI GGGAC 1 cut(s) 246
FatI CATG 4 cut(s) 117, 267, 435, 460
Fnu4HI GCNGC 5 cut(s) 273, 306, 339, 470, 503
FokI GGATG 2 cut(s) 623, 713
FriOI GRGCYC 1 cut(s) 331
Fsp4HI GCNGC 5 cut(s) 273, 306, 339, 470, 503
FspBI CTAG 1 cut(s) 727
GluI GCNGC 5 cut(s) 273, 306, 339, 470, 503
GsuI CTGGAG 1 cut(s) 345
HaeIII GGCC 2 cut(s) 110, 272
Hin1II CATG 4 cut(s) 121, 271, 439, 464
HindIII AAGCTT 2 cut(s) 485, 632
HinfI GANTC 2 cut(s) 284, 371
HphI GGTGA 1 cut(s) 95
Hpy166II GTNNAC 1 cut(s) 385
Hpy188I TCNGA 2 cut(s) 446, 547
Hpy188III TCNNGA 6 cut(s) 314, 344, 362, 386, 461, 476
Hpy8I GTNNAC 1 cut(s) 385
Hpy99I CGWCG 1 cut(s) 226
HpyAV CCTTC 1 cut(s) 246
HpyCH4III ACNGT 1 cut(s) 351
HpyCH4IV ACGT 2 cut(s) 72, 310
HpyCH4V TGCA 6 cut(s) 4, 212, 217, 410, 527, 616
HpyF10VI GCNNNNNNNGC 8 cut(s) 46, 83, 272, 305, 335, 622, 631, 640
HpyF3I CTNAG 3 cut(s) 82, 323, 342
HpySE526I ACGT 2 cut(s) 72, 310
Hsp92II CATG 4 cut(s) 121, 271, 439, 464
LmnI GCTCC 1 cut(s) 583
Lsp1109I GCAGC 4 cut(s) 317, 350, 481, 489
MaeI CTAG 1 cut(s) 727
MaeII ACGT 2 cut(s) 72, 310
MaeIII GTNAC 2 cut(s) 194, 416
MboII GAAGA 7 cut(s) 168, 233, 239, 290, 527, 562, 631
MfeI CAATTG 1 cut(s) 695
MhlI GDGCHC 2 cut(s) 42, 331
MluCI AATT 5 cut(s) 165, 172, 411, 440, 695
MlyI GAGTC 1 cut(s) 278
MseI TTAA 5 cut(s) 66, 204, 333, 353, 663
MspA1I CMGCKG 1 cut(s) 275
MspR9I CCNGG 1 cut(s) 107
MunI CAATTG 1 cut(s) 695
MvaI CCWGG 1 cut(s) 107
MwoI GCNNNNNNNGC 8 cut(s) 46, 83, 272, 305, 335, 622, 631, 640
NcoI CCATGG 1 cut(s) 267
NlaIII CATG 4 cut(s) 121, 271, 439, 464
NlaIV GGNNCC 2 cut(s) 234, 561
NmuCI GTSAC 2 cut(s) 194, 416
PagI TCATGA 1 cut(s) 460
PfeI GAWTC 1 cut(s) 371
PkrI GCNGC 5 cut(s) 274, 307, 340, 471, 504
PleI GAGTC 1 cut(s) 278
PpsI GAGTC 1 cut(s) 278
Ppu21I YACGTR 1 cut(s) 73
PpuMI RGGWCCY 2 cut(s) 233, 559
Psp124BI GAGCTC 1 cut(s) 331
Psp5II RGGWCCY 2 cut(s) 233, 559
Psp6I CCWGG 1 cut(s) 105
PspGI CCWGG 1 cut(s) 105
PspN4I GGNNCC 2 cut(s) 234, 561
PspPI GGNCC 3 cut(s) 109, 233, 559
PspPPI RGGWCCY 2 cut(s) 233, 559
PstNI CAGNNNCTG 1 cut(s) 349
RsaI GTAC 1 cut(s) 534
RsaNI GTAC 1 cut(s) 533
SacI GAGCTC 1 cut(s) 331
SaqAI TTAA 5 cut(s) 66, 204, 333, 353, 663
SatI GCNGC 5 cut(s) 273, 306, 339, 470, 503
Sau96I GGNCC 3 cut(s) 109, 233, 559
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 1 cut(s) 107
SduI GDGCHC 2 cut(s) 42, 331
SinI GGWCC 2 cut(s) 233, 559
Sse9I AATT 5 cut(s) 165, 172, 411, 440, 695
SsiI CCGC 3 cut(s) 253, 273, 375
SspI AATATT 1 cut(s) 574
SspMI CTAG 1 cut(s) 727
SstI GAGCTC 1 cut(s) 331
StyD4I CCNGG 1 cut(s) 105
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 1 cut(s) 351
TaiI ACGT 2 cut(s) 75, 313
TasI AATT 5 cut(s) 165, 172, 411, 440, 695
TauI GCSGC 1 cut(s) 275
TfiI GAWTC 1 cut(s) 371
Tru1I TTAA 5 cut(s) 66, 204, 333, 353, 663
Tru9I TTAA 5 cut(s) 66, 204, 333, 353, 663
TscAI CASTG 1 cut(s) 504
TseFI GTSAC 2 cut(s) 194, 416
TseI GCWGC 4 cut(s) 305, 338, 469, 502
Tsp45I GTSAC 2 cut(s) 194, 416
TspDTI ATGAA 2 cut(s) 414, 452
TspRI CASTG 1 cut(s) 504
VpaK11BI GGWCC 2 cut(s) 233, 559
XagI CCTNNNNNAGG 1 cut(s) 525
XapI RAATTY 2 cut(s) 165, 440
XspI CTAG 1 cut(s) 727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.