MD14G1246600.v1.1

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005457
Physical Location & Seq
Reverse (-)
32431767 .. 32434489
2723 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1246600.v1.1.491

Sequence Viewer

Length: 642 bp
ATGCTGTCCTGTGGTCGGCTGTGTCTTTCTATTCCAGTCTCCACCTCTTTTGTTTCTCCTCCTTCCTCTCAAATTCTCAATTCTTCCATTCCATTCCACAGTGACAGTGTTAACAGTGCATTGCAGCGCCGCAGACGACGAGTCCGGGGCCTTCTCTTCGTCTTCTCCGGTGAAGCAATGGCCGTTGAAGAGGACTCCGTTGAACAAGTACTTGCAGCGCGTCAAGAGAGGCTAAGAGCTCTTAAAGCTGCTCAGGAACTGTTAAACACTCCAGATGAGGATTCCGCTCAAGCTCACGATAACACTAATGAAAACAATGCAATTAGTGACGAAGCTAATGCCAACATGAAATTCCGAAATTATGTTCCTCATGATAAGCAGCTTCAGGGGGGAAAGCTTGTTCCACCGGTGCTGCCAAAGTTTGAAGACCCTGTTGCAGCAGTACCTCCTCTATCAGAGAAGAAAGAGGACCCATTTGTGAATATTGCTCCTAAAAAACCAAACTGGGAACTTCGCAGGGATGTGCAGAAGAGGCTTGATAAGCTTGAAAAGCGAACACAAAAGGCAATGCTTAAACTTATGGGGGAACAAGAGAAGCAAAAGCAATTGGATAGAGATGGCGGAAATGGTGGAGAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
cwf18

Protein Analysis

214

Amino Acids

23.84

Weight (kDa)

7.76

Isoelectric Point (pI)

65.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cwf18 PF08315 67 - 196 1.2e-28 cwf18 pre-mRNA splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 287
AccII CGCG 1 cut(s) 220
AciI CCGC 3 cut(s) 130, 285, 621
AcoI YGGCCR 1 cut(s) 180
AcsI RAATTY 2 cut(s) 72, 350
AcuI CTGAAG 1 cut(s) 368
AfaI GTAC 2 cut(s) 210, 444
AfiI CCNNNNNNNGG 1 cut(s) 15
AgeI ACCGGT 1 cut(s) 406
AgsI TTSAA 4 cut(s) 188, 203, 425, 548
AhdI GACNNNNNGTC 1 cut(s) 140
AjuI GAANNNNNNNTTGG 2 cut(s) 335, 367
AloI GAACNNNNNNTCC 2 cut(s) 384, 416
AluBI AGCT 7 cut(s) 239, 248, 293, 335, 382, 397, 544
AluI AGCT 7 cut(s) 239, 248, 293, 335, 382, 397, 544
Alw21I GWGCWC 1 cut(s) 241
Alw26I GTCTC 1 cut(s) 43
AlwNI CAGNNNCTG 1 cut(s) 259
AoxI GGCC 2 cut(s) 148, 180
ApeKI GCWGC 6 cut(s) 124, 215, 248, 379, 412, 437
ApoI RAATTY 2 cut(s) 72, 350
AsiGI ACCGGT 1 cut(s) 406
AspLEI GCGC 2 cut(s) 129, 220
AspS9I GGNCC 2 cut(s) 148, 469
AsuC2I CCSGG 1 cut(s) 146
AsuHPI GGTGA 1 cut(s) 182
AvaII GGWCC 1 cut(s) 469
BanII GRGCYC 1 cut(s) 241
BbsI GAAGAC 2 cut(s) 154, 432
Bbv12I GWGCWC 1 cut(s) 241
BbvI GCAGC 6 cut(s) 136, 227, 235, 391, 399, 449
BccI CCATC 1 cut(s) 611
BceAI ACGGC 1 cut(s) 167
BcgI CGANNNNNNTGC 2 cut(s) 320, 354
BcnI CCSGG 1 cut(s) 146
BcoDI GTCTC 1 cut(s) 43
BfaI CTAG 1 cut(s) 640
BfoI RGCGCY 1 cut(s) 130
BisI GCNGC 7 cut(s) 125, 130, 216, 249, 380, 413, 438
BlsI GCNGC 7 cut(s) 126, 131, 217, 250, 381, 414, 439
BmcAI AGTACT 1 cut(s) 210
Bme1390I CCNGG 1 cut(s) 146
Bme18I GGWCC 1 cut(s) 469
BmeRI GACNNNNNGTC 1 cut(s) 140
BmgT120I GGNCC 2 cut(s) 148, 469
BmiI GGNNCC 2 cut(s) 149, 471
BmrFI CCNGG 1 cut(s) 146
BmrI ACTGGG 1 cut(s) 514
BmuI ACTGGG 1 cut(s) 514
BpiI GAAGAC 2 cut(s) 154, 432
BpmI CTGGAG 1 cut(s) 255
Bpu10I CCTNAGC 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 273
BpuMI CCSGG 1 cut(s) 146
BsaJI CCNNGG 1 cut(s) 145
BsaWI WCCGGW 2 cut(s) 167, 406
Bsc4I CCNNNNNNNGG 1 cut(s) 15
Bse118I RCCGGY 1 cut(s) 406
Bse1I ACTGG 2 cut(s) 35, 509
Bse3DI GCAATG 3 cut(s) 119, 183, 573
BseDI CCNNGG 1 cut(s) 145
BseGI GGATG 1 cut(s) 526
BseLI CCNNNNNNNGG 1 cut(s) 15
BseMI GCAATG 3 cut(s) 119, 183, 573
BseMII CTCAG 1 cut(s) 266
BseNI ACTGG 2 cut(s) 35, 509
BseRI GAGGAG 2 cut(s) 48, 438
BseXI GCAGC 6 cut(s) 136, 227, 235, 391, 399, 449
BsgI GTGCAG 1 cut(s) 545
Bsh1236I CGCG 1 cut(s) 220
BshFI GGCC 2 cut(s) 150, 182
BshTI ACCGGT 1 cut(s) 406
BsiHKAI GWGCWC 1 cut(s) 241
BsiSI CCGG 3 cut(s) 145, 168, 407
BslI CCNNNNNNNGG 1 cut(s) 15
BsmAI GTCTC 1 cut(s) 43
BsnI GGCC 2 cut(s) 150, 182
Bsp1286I GDGCHC 1 cut(s) 241
BspACI CCGC 3 cut(s) 130, 285, 621
BspANI GGCC 2 cut(s) 150, 182
BspCNI CTCAG 1 cut(s) 265
BspFNI CGCG 1 cut(s) 220
BspHI TCATGA 1 cut(s) 370
BspLI GGNNCC 2 cut(s) 149, 471
BsrBI CCGCTC 1 cut(s) 287
BsrDI GCAATG 3 cut(s) 119, 183, 573
BsrFI RCCGGY 1 cut(s) 406
BsrI ACTGG 2 cut(s) 35, 509
BssAI RCCGGY 1 cut(s) 406
BssECI CCNNGG 1 cut(s) 145
Bst4CI ACNGT 4 cut(s) 101, 107, 116, 261
Bst6I CTCTTC 3 cut(s) 161, 183, 524
BstDEI CTNAG 2 cut(s) 233, 252
BstF5I GGATG 1 cut(s) 526
BstFNI CGCG 1 cut(s) 220
BstH2I RGCGCY 1 cut(s) 130
BstHHI GCGC 2 cut(s) 129, 220
BstMAI GTCTC 1 cut(s) 43
BstMWI GCNNNNNNNGC 4 cut(s) 245, 532, 541, 550
BstSCI CCNGG 1 cut(s) 144
BstUI CGCG 1 cut(s) 220
BstV1I GCAGC 6 cut(s) 136, 227, 235, 391, 399, 449
BstV2I GAAGAC 2 cut(s) 154, 432
BsuRI GGCC 2 cut(s) 150, 182
BtsCI GGATG 1 cut(s) 526
BtsIMutI CAGTG 3 cut(s) 106, 112, 121
CaiI CAGNNNCTG 1 cut(s) 259
CciI TCATGA 1 cut(s) 370
CfoI GCGC 2 cut(s) 129, 220
Cfr10I RCCGGY 1 cut(s) 406
Cfr13I GGNCC 2 cut(s) 148, 469
CseI GACGC 1 cut(s) 209
Csp6I GTAC 2 cut(s) 209, 443
CspAI ACCGGT 1 cut(s) 406
CspCI CAANNNNNGTGG 2 cut(s) 31, 66
CviAII CATG 2 cut(s) 346, 371
CviQI GTAC 2 cut(s) 209, 443
DdeI CTNAG 2 cut(s) 233, 252
DriI GACNNNNNGTC 1 cut(s) 140
EaeI YGGCCR 1 cut(s) 180
Eam1104I CTCTTC 3 cut(s) 161, 183, 524
Eam1105I GACNNNNNGTC 1 cut(s) 140
EarI CTCTTC 3 cut(s) 161, 183, 524
EciI GGCGGA 1 cut(s) 636
Ecl136II GAGCTC 1 cut(s) 239
Eco24I GRGCYC 1 cut(s) 241
Eco47I GGWCC 1 cut(s) 469
Eco53kI GAGCTC 1 cut(s) 239
Eco57I CTGAAG 1 cut(s) 368
EcoICRI GAGCTC 1 cut(s) 239
EcoO109I RGGNCCY 2 cut(s) 148, 469
EcoT38I GRGCYC 1 cut(s) 241
FaeI CATG 2 cut(s) 349, 374
FaiI YATR 4 cut(s) 347, 363, 372, 581
FatI CATG 2 cut(s) 345, 370
Fnu4HI GCNGC 7 cut(s) 125, 130, 216, 249, 380, 413, 438
FokI GGATG 1 cut(s) 533
FriOI GRGCYC 1 cut(s) 241
Fsp4HI GCNGC 7 cut(s) 125, 130, 216, 249, 380, 413, 438
FspBI CTAG 1 cut(s) 640
GlaI GCGC 2 cut(s) 128, 219
GluI GCNGC 7 cut(s) 125, 130, 216, 249, 380, 413, 438
GsuI CTGGAG 1 cut(s) 255
HaeII RGCGCY 1 cut(s) 130
HaeIII GGCC 2 cut(s) 150, 182
HapII CCGG 3 cut(s) 145, 168, 407
HgaI GACGC 1 cut(s) 209
HhaI GCGC 2 cut(s) 129, 220
Hin1II CATG 2 cut(s) 349, 374
Hin6I GCGC 2 cut(s) 127, 218
HinP1I GCGC 2 cut(s) 127, 218
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HindIII AAGCTT 2 cut(s) 395, 542
HinfI GANTC 3 cut(s) 141, 194, 281
HpaI GTTAAC 1 cut(s) 112
HpaII CCGG 3 cut(s) 145, 168, 407
HphI GGTGA 1 cut(s) 182
Hpy166II GTNNAC 1 cut(s) 112
Hpy188I TCNGA 2 cut(s) 356, 457
Hpy188III TCNNGA 5 cut(s) 224, 254, 272, 296, 371
Hpy8I GTNNAC 1 cut(s) 112
Hpy99I CGWCG 1 cut(s) 141
HpyAV CCTTC 2 cut(s) 72, 161
HpyCH4III ACNGT 4 cut(s) 101, 107, 116, 261
HpyCH4V TGCA 6 cut(s) 119, 124, 215, 320, 437, 526
HpyF10VI GCNNNNNNNGC 4 cut(s) 245, 532, 541, 550
HpyF3I CTNAG 2 cut(s) 233, 252
Hsp92II CATG 2 cut(s) 349, 374
HspAI GCGC 2 cut(s) 127, 218
KspAI GTTAAC 1 cut(s) 112
LmnI GCTCC 1 cut(s) 493
Lsp1109I GCAGC 6 cut(s) 136, 227, 235, 391, 399, 449
MaeI CTAG 1 cut(s) 640
MaeIII GTNAC 2 cut(s) 101, 326
MbiI CCGCTC 1 cut(s) 287
MboII GAAGA 7 cut(s) 75, 148, 154, 200, 437, 472, 541
MfeI CAATTG 1 cut(s) 605
MhlI GDGCHC 1 cut(s) 241
MluCI AATT 6 cut(s) 72, 79, 321, 350, 358, 605
MlyI GAGTC 2 cut(s) 150, 188
MseI TTAA 4 cut(s) 111, 243, 263, 573
MspI CCGG 3 cut(s) 145, 168, 407
MspR9I CCNGG 1 cut(s) 146
MunI CAATTG 1 cut(s) 605
MvnI CGCG 1 cut(s) 220
MwoI GCNNNNNNNGC 4 cut(s) 245, 532, 541, 550
NciI CCSGG 1 cut(s) 146
NlaIII CATG 2 cut(s) 349, 374
NlaIV GGNNCC 2 cut(s) 149, 471
NmuCI GTSAC 2 cut(s) 101, 326
PagI TCATGA 1 cut(s) 370
PfeI GAWTC 1 cut(s) 281
PinAI ACCGGT 1 cut(s) 406
PkrI GCNGC 7 cut(s) 126, 131, 217, 250, 381, 414, 439
PleI GAGTC 2 cut(s) 149, 188
PpsI GAGTC 2 cut(s) 149, 188
PpuMI RGGWCCY 1 cut(s) 469
Psp124BI GAGCTC 1 cut(s) 241
Psp5II RGGWCCY 1 cut(s) 469
PspN4I GGNNCC 2 cut(s) 149, 471
PspPI GGNCC 2 cut(s) 148, 469
PspPPI RGGWCCY 1 cut(s) 469
PstNI CAGNNNCTG 1 cut(s) 259
RsaI GTAC 2 cut(s) 210, 444
RsaNI GTAC 2 cut(s) 209, 443
SacI GAGCTC 1 cut(s) 241
SaqAI TTAA 4 cut(s) 111, 243, 263, 573
SatI GCNGC 7 cut(s) 125, 130, 216, 249, 380, 413, 438
Sau96I GGNCC 2 cut(s) 148, 469
ScaI AGTACT 1 cut(s) 210
SchI GAGTC 2 cut(s) 150, 188
ScrFI CCNGG 1 cut(s) 146
SduI GDGCHC 1 cut(s) 241
SetI ASST 9 cut(s) 47, 241, 250, 295, 337, 384, 399, 448, 546
SgrAI CRCCGGYG 1 cut(s) 406
SinI GGWCC 1 cut(s) 469
SmlI CTYRAG 1 cut(s) 288
SmoI CTYRAG 1 cut(s) 288
Sse9I AATT 6 cut(s) 72, 79, 321, 350, 358, 605
SsiI CCGC 3 cut(s) 130, 285, 621
SspI AATATT 1 cut(s) 484
SspMI CTAG 1 cut(s) 640
SstI GAGCTC 1 cut(s) 241
StyD4I CCNGG 1 cut(s) 144
TaaI ACNGT 4 cut(s) 101, 107, 116, 261
TasI AATT 6 cut(s) 72, 79, 321, 350, 358, 605
TatI WGTACW 1 cut(s) 208
TauI GCSGC 1 cut(s) 132
TfiI GAWTC 1 cut(s) 281
Tru1I TTAA 4 cut(s) 111, 243, 263, 573
Tru9I TTAA 4 cut(s) 111, 243, 263, 573
TscAI CASTG 3 cut(s) 106, 112, 121
TseFI GTSAC 2 cut(s) 101, 326
TseI GCWGC 6 cut(s) 124, 215, 248, 379, 412, 437
Tsp45I GTSAC 2 cut(s) 101, 326
TspDTI ATGAA 2 cut(s) 324, 362
TspGWI ACGGA 1 cut(s) 187
TspRI CASTG 3 cut(s) 106, 112, 121
VpaK11BI GGWCC 1 cut(s) 469
XapI RAATTY 2 cut(s) 72, 350
XspI CTAG 1 cut(s) 640
ZrmI AGTACT 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.