MD06G1240400.v1.1

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0005457
Physical Location & Seq
Reverse (-)
37038014 .. 37040931
2918 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1240400.v1.1.491

Sequence Viewer

Length: 630 bp
ATGCTGTGGTGTGGTCGGTCTGTATCTTTCTTGTGCAGTTTACCACCTCTTTTGTTTCTCCTCCCTCTCAAATTCTCAATTCTGCGATTCCATTCCAAAGTGACAGCGTTAACAGTGCATTGCAGACGACGGGTCAGGGACCTTCTCTTCGTCTTCTCCGGCGAAGCCATGGCCGCTGAAGAGGACTCCATTGAACAAGCAGTCGCAGCACGTCAAGAGAGGCTAAGAGCTCTTAAAGCAGCTCAGGAACTGTTAAACACTCCAGATGAGGATTCCGCACAAGTTCACGATAACACTAATGAAAGCAATCCAATTAGTGACGAAACTAATACCAACATGAAATTCCGAAATTATGTTCCTCATGATAAGCAGCTTCAGGAGGGAAAGCTTGTTCCACCAGTGCTGCCAAAGTTTGAAGACCCTGTTGCAGGAGTACCACCTCCATCAGAGAAGAAAGAGGACCCGTTTGTGAATATTGCTCCTAAAAAACCAAACTGGGAACTTCGCAGGGATGTGCAGAAGAGGCTTGATAAGCTTGAAAAGCGAACCCAAAAGGCAATGCTTAAACTTATGGAGGAACAGGAGAAGCAAAAGCAATTGGATGGAGATGGCAGAAATGGTGAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
cwf18

Protein Analysis

210

Amino Acids

23.94

Weight (kDa)

6.92

Isoelectric Point (pI)

59.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cwf18 PF08315 63 - 193 2.5e-30 cwf18 pre-mRNA splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 174, 276
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 2 cut(s) 71, 341
AcuI CTGAAG 2 cut(s) 198, 359
AfaI GTAC 1 cut(s) 435
AfiI CCNNNNNNNGG 1 cut(s) 428
AgsI TTSAA 3 cut(s) 194, 416, 539
AhdI GACNNNNNGTC 1 cut(s) 131
AjiI CACGTC 1 cut(s) 212
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
AloI GAACNNNNNNTCC 2 cut(s) 375, 407
AluBI AGCT 5 cut(s) 230, 242, 373, 388, 535
AluI AGCT 5 cut(s) 230, 242, 373, 388, 535
Alw21I GWGCWC 1 cut(s) 232
AlwNI CAGNNNCTG 1 cut(s) 250
AoxI GGCC 1 cut(s) 171
ApeKI GCWGC 4 cut(s) 206, 239, 370, 403
ApoI RAATTY 2 cut(s) 71, 341
ArsI GACNNNNNNTTYG 2 cut(s) 131, 163
AspS9I GGNCC 2 cut(s) 139, 460
AvaII GGWCC 2 cut(s) 139, 460
BanII GRGCYC 1 cut(s) 232
BbsI GAAGAC 2 cut(s) 145, 423
Bbv12I GWGCWC 1 cut(s) 232
BbvI GCAGC 4 cut(s) 218, 251, 382, 390
BccI CCATC 3 cut(s) 451, 596, 602
BfaI CTAG 1 cut(s) 628
BisI GCNGC 5 cut(s) 174, 207, 240, 371, 404
BlsI GCNGC 5 cut(s) 175, 208, 241, 372, 405
Bme18I GGWCC 2 cut(s) 139, 460
BmeRI GACNNNNNGTC 1 cut(s) 131
BmgBI CACGTC 1 cut(s) 212
BmgT120I GGNCC 2 cut(s) 139, 460
BmiI GGNNCC 2 cut(s) 140, 462
BmrI ACTGGG 1 cut(s) 505
BmuI ACTGGG 1 cut(s) 505
BpiI GAAGAC 2 cut(s) 145, 423
BpmI CTGGAG 1 cut(s) 246
Bpu10I CCTNAGC 1 cut(s) 243
BsaJI CCNNGG 1 cut(s) 168
Bsc4I CCNNNNNNNGG 1 cut(s) 428
Bse1I ACTGG 2 cut(s) 398, 500
Bse3DI GCAATG 2 cut(s) 118, 564
BseDI CCNNGG 1 cut(s) 168
BseGI GGATG 2 cut(s) 517, 607
BseLI CCNNNNNNNGG 1 cut(s) 428
BseMI GCAATG 2 cut(s) 118, 564
BseMII CTCAG 1 cut(s) 257
BseNI ACTGG 2 cut(s) 398, 500
BseRI GAGGAG 1 cut(s) 50
BseXI GCAGC 4 cut(s) 218, 251, 382, 390
BsgI GTGCAG 2 cut(s) 55, 536
BshFI GGCC 1 cut(s) 173
BsiHKAI GWGCWC 1 cut(s) 232
BsiSI CCGG 1 cut(s) 159
BslFI GGGAC 1 cut(s) 152
BslI CCNNNNNNNGG 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 152
BsnI GGCC 1 cut(s) 173
Bsp1286I GDGCHC 1 cut(s) 232
Bsp19I CCATGG 1 cut(s) 168
BspACI CCGC 2 cut(s) 174, 276
BspANI GGCC 1 cut(s) 173
BspCNI CTCAG 1 cut(s) 256
BspHI TCATGA 1 cut(s) 361
BspLI GGNNCC 2 cut(s) 140, 462
BsrDI GCAATG 2 cut(s) 118, 564
BsrI ACTGG 2 cut(s) 398, 500
BssECI CCNNGG 1 cut(s) 168
BssT1I CCWWGG 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 115, 252
Bst6I CTCTTC 3 cut(s) 152, 174, 515
BstDEI CTNAG 2 cut(s) 224, 243
BstDSI CCRYGG 1 cut(s) 168
BstENI CCTNNNNNAGG 1 cut(s) 426
BstF5I GGATG 2 cut(s) 517, 607
BstMWI GCNNNNNNNGC 6 cut(s) 173, 206, 236, 523, 532, 541
BstV1I GCAGC 4 cut(s) 218, 251, 382, 390
BstV2I GAAGAC 2 cut(s) 145, 423
BsuRI GGCC 1 cut(s) 173
BtgI CCRYGG 1 cut(s) 168
BtrI CACGTC 1 cut(s) 212
BtsCI GGATG 2 cut(s) 517, 607
BtsIMutI CAGTG 2 cut(s) 120, 405
CaiI CAGNNNCTG 1 cut(s) 250
CciI TCATGA 1 cut(s) 361
Cfr13I GGNCC 2 cut(s) 139, 460
Csp6I GTAC 1 cut(s) 434
CspCI CAANNNNNGTGG 2 cut(s) 33, 68
CviAII CATG 3 cut(s) 169, 337, 362
CviJI RGCY 9 cut(s) 167, 173, 223, 230, 242, 373, 388, 526, 535
CviKI_1 RGCY 9 cut(s) 167, 173, 223, 230, 242, 373, 388, 526, 535
CviQI GTAC 1 cut(s) 434
DdeI CTNAG 2 cut(s) 224, 243
DriI GACNNNNNGTC 1 cut(s) 131
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 3 cut(s) 152, 174, 515
Eam1105I GACNNNNNGTC 1 cut(s) 131
EarI CTCTTC 3 cut(s) 152, 174, 515
Ecl136II GAGCTC 1 cut(s) 230
Eco130I CCWWGG 1 cut(s) 168
Eco24I GRGCYC 1 cut(s) 232
Eco47I GGWCC 2 cut(s) 139, 460
Eco53kI GAGCTC 1 cut(s) 230
Eco57I CTGAAG 2 cut(s) 198, 359
EcoICRI GAGCTC 1 cut(s) 230
EcoNI CCTNNNNNAGG 1 cut(s) 426
EcoO109I RGGNCCY 2 cut(s) 139, 460
EcoT14I CCWWGG 1 cut(s) 168
EcoT38I GRGCYC 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 168
FaeI CATG 3 cut(s) 172, 340, 365
FaiI YATR 5 cut(s) 170, 338, 354, 363, 572
FaqI GGGAC 1 cut(s) 152
FatI CATG 3 cut(s) 168, 336, 361
Fnu4HI GCNGC 5 cut(s) 174, 207, 240, 371, 404
FokI GGATG 2 cut(s) 524, 614
FriOI GRGCYC 1 cut(s) 232
Fsp4HI GCNGC 5 cut(s) 174, 207, 240, 371, 404
FspBI CTAG 1 cut(s) 628
GluI GCNGC 5 cut(s) 174, 207, 240, 371, 404
GsuI CTGGAG 1 cut(s) 246
HaeIII GGCC 1 cut(s) 173
HapII CCGG 1 cut(s) 159
Hin1II CATG 3 cut(s) 172, 340, 365
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HindIII AAGCTT 2 cut(s) 386, 533
HinfI GANTC 3 cut(s) 87, 185, 272
HpaI GTTAAC 1 cut(s) 111
HpaII CCGG 1 cut(s) 159
Hpy166II GTNNAC 3 cut(s) 41, 111, 286
Hpy188I TCNGA 2 cut(s) 347, 448
Hpy188III TCNNGA 6 cut(s) 215, 245, 263, 287, 362, 377
Hpy8I GTNNAC 3 cut(s) 41, 111, 286
Hpy99I CGWCG 1 cut(s) 132
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 2 cut(s) 115, 252
HpyCH4IV ACGT 1 cut(s) 211
HpyCH4V TGCA 5 cut(s) 36, 118, 123, 428, 517
HpyF10VI GCNNNNNNNGC 6 cut(s) 173, 206, 236, 523, 532, 541
HpyF3I CTNAG 2 cut(s) 224, 243
HpySE526I ACGT 1 cut(s) 211
Hsp92II CATG 3 cut(s) 172, 340, 365
KspAI GTTAAC 1 cut(s) 111
LmnI GCTCC 1 cut(s) 484
Lsp1109I GCAGC 4 cut(s) 218, 251, 382, 390
MaeI CTAG 1 cut(s) 628
MaeII ACGT 1 cut(s) 211
MaeIII GTNAC 2 cut(s) 100, 317
MboII GAAGA 6 cut(s) 139, 145, 191, 428, 463, 532
MfeI CAATTG 1 cut(s) 596
MhlI GDGCHC 1 cut(s) 232
MluCI AATT 6 cut(s) 71, 78, 312, 341, 349, 596
MlyI GAGTC 1 cut(s) 179
MseI TTAA 4 cut(s) 110, 234, 254, 564
MspA1I CMGCKG 1 cut(s) 176
MspI CCGG 1 cut(s) 159
MunI CAATTG 1 cut(s) 596
MwoI GCNNNNNNNGC 6 cut(s) 173, 206, 236, 523, 532, 541
NcoI CCATGG 1 cut(s) 168
NlaIII CATG 3 cut(s) 172, 340, 365
NlaIV GGNNCC 2 cut(s) 140, 462
NmuCI GTSAC 2 cut(s) 100, 317
PagI TCATGA 1 cut(s) 361
PfeI GAWTC 2 cut(s) 87, 272
PkrI GCNGC 5 cut(s) 175, 208, 241, 372, 405
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
PpuMI RGGWCCY 2 cut(s) 139, 460
Psp124BI GAGCTC 1 cut(s) 232
Psp5II RGGWCCY 2 cut(s) 139, 460
PspN4I GGNNCC 2 cut(s) 140, 462
PspPI GGNCC 2 cut(s) 139, 460
PspPPI RGGWCCY 2 cut(s) 139, 460
PstNI CAGNNNCTG 1 cut(s) 250
RsaI GTAC 1 cut(s) 435
RsaNI GTAC 1 cut(s) 434
SacI GAGCTC 1 cut(s) 232
SaqAI TTAA 4 cut(s) 110, 234, 254, 564
SatI GCNGC 5 cut(s) 174, 207, 240, 371, 404
Sau96I GGNCC 2 cut(s) 139, 460
SchI GAGTC 1 cut(s) 179
SduI GDGCHC 1 cut(s) 232
SetI ASST 9 cut(s) 49, 144, 214, 232, 244, 375, 390, 442, 537
SinI GGWCC 2 cut(s) 139, 460
Sse9I AATT 6 cut(s) 71, 78, 312, 341, 349, 596
SsiI CCGC 2 cut(s) 174, 276
SspI AATATT 1 cut(s) 475
SspMI CTAG 1 cut(s) 628
SstI GAGCTC 1 cut(s) 232
StyI CCWWGG 1 cut(s) 168
TaaI ACNGT 2 cut(s) 115, 252
TaiI ACGT 1 cut(s) 214
TaqII GACCGA 1 cut(s) 6
TasI AATT 6 cut(s) 71, 78, 312, 341, 349, 596
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 2 cut(s) 87, 272
Tru1I TTAA 4 cut(s) 110, 234, 254, 564
Tru9I TTAA 4 cut(s) 110, 234, 254, 564
TscAI CASTG 2 cut(s) 120, 405
TseFI GTSAC 2 cut(s) 100, 317
TseI GCWGC 4 cut(s) 206, 239, 370, 403
Tsp45I GTSAC 2 cut(s) 100, 317
TspDTI ATGAA 2 cut(s) 315, 353
TspRI CASTG 2 cut(s) 120, 405
VpaK11BI GGWCC 2 cut(s) 139, 460
XagI CCTNNNNNAGG 1 cut(s) 426
XapI RAATTY 2 cut(s) 71, 341
XspI CTAG 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.