AT3G10860

Cytochrome b-c1 complex subunit 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004343
Physical Location & Seq
Forward (+)
3399550 .. 3400926
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G10860.1

Sequence Viewer

Length: 219 bp
ATGGGGAAGCAGCCGGTGAAATTGAAGGCGGTGGTTTACGCACTTTCTCCGTTTCAGCAGAAGATCATGACCGGTCTCTGGAAGGATCTGCCGGAGAAGATCCACCACAAGGTCTCTGAGAATTGGATCAGCGCCACTCTTCTCGTTACCCCTGTCGTTGGAACCTACTGGTATGCTCAGTATTTCAAGGAACAGGAGAAGCTTGAGCACAGATTCTGA

Protein Analysis

72

Amino Acids

8.5

Weight (kDa)

9.48

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt_b-c1_8 PF10890 1 - 72 7.1e-39 Cytochrome b-c1 complex subunit 8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 29
AclWI GGATC 3 cut(s) 93, 94, 134
AfiI CCNNNNNNNGG 3 cut(s) 78, 109, 158
AgeI ACCGGT 1 cut(s) 71
AgsI TTSAA 2 cut(s) 25, 187
AluBI AGCT 1 cut(s) 202
AluI AGCT 1 cut(s) 202
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 2 cut(s) 80, 118
AlwI GGATC 3 cut(s) 93, 94, 134
AlwNI CAGNNNCTG 1 cut(s) 216
ApeKI GCWGC 1 cut(s) 10
AsiGI ACCGGT 1 cut(s) 71
AspLEI GCGC 1 cut(s) 134
AsuHPI GGTGA 1 cut(s) 28
Bbv12I GWGCWC 1 cut(s) 210
BbvI GCAGC 1 cut(s) 22
BcoDI GTCTC 2 cut(s) 80, 118
BfoI RGCGCY 1 cut(s) 135
BisI GCNGC 1 cut(s) 11
BlsI GCNGC 1 cut(s) 12
BmiI GGNNCC 1 cut(s) 163
BsaI GGTCTC 2 cut(s) 80, 118
BsaWI WCCGGW 1 cut(s) 71
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 109, 158
Bse118I RCCGGY 2 cut(s) 13, 71
Bse1I ACTGG 1 cut(s) 173
BseLI CCNNNNNNNGG 3 cut(s) 78, 109, 158
BseMII CTCAG 2 cut(s) 108, 191
BseNI ACTGG 1 cut(s) 173
BseXI GCAGC 1 cut(s) 22
BshTI ACCGGT 1 cut(s) 71
BsiHKAI GWGCWC 1 cut(s) 210
BsiSI CCGG 3 cut(s) 14, 72, 92
BslI CCNNNNNNNGG 3 cut(s) 78, 109, 158
BsmAI GTCTC 2 cut(s) 80, 118
Bso31I GGTCTC 2 cut(s) 80, 118
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 4 cut(s) 63, 85, 99, 126
BspACI CCGC 1 cut(s) 29
BspCNI CTCAG 2 cut(s) 109, 190
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 1 cut(s) 163
BspPI GGATC 3 cut(s) 93, 94, 134
BspTNI GGTCTC 2 cut(s) 80, 118
BsrFI RCCGGY 2 cut(s) 13, 71
BsrI ACTGG 1 cut(s) 173
BssAI RCCGGY 2 cut(s) 13, 71
BssMI GATC 4 cut(s) 63, 85, 99, 126
Bst6I CTCTTC 1 cut(s) 144
BstDEI CTNAG 2 cut(s) 117, 177
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 1 cut(s) 134
BstKTI GATC 4 cut(s) 66, 88, 102, 129
BstMAI GTCTC 2 cut(s) 80, 118
BstMBI GATC 4 cut(s) 63, 85, 99, 126
BstV1I GCAGC 1 cut(s) 22
BstX2I RGATCY 2 cut(s) 85, 99
BstYI RGATCY 2 cut(s) 85, 99
CaiI CAGNNNCTG 1 cut(s) 216
CciI TCATGA 1 cut(s) 66
CfoI GCGC 1 cut(s) 134
Cfr10I RCCGGY 2 cut(s) 13, 71
CspAI ACCGGT 1 cut(s) 71
CviAII CATG 1 cut(s) 67
CviJI RGCY 2 cut(s) 13, 202
CviKI_1 RGCY 2 cut(s) 13, 202
DdeI CTNAG 2 cut(s) 117, 177
DpnI GATC 4 cut(s) 65, 87, 101, 128
DpnII GATC 4 cut(s) 63, 85, 99, 126
Eam1104I CTCTTC 1 cut(s) 144
EarI CTCTTC 1 cut(s) 144
Eco31I GGTCTC 2 cut(s) 80, 118
FaeI CATG 1 cut(s) 70
FaiI YATR 2 cut(s) 68, 174
FatI CATG 1 cut(s) 66
Fnu4HI GCNGC 1 cut(s) 11
Fsp4HI GCNGC 1 cut(s) 11
GlaI GCGC 1 cut(s) 133
GluI GCNGC 1 cut(s) 11
HaeII RGCGCY 1 cut(s) 135
HapII CCGG 3 cut(s) 14, 72, 92
HhaI GCGC 1 cut(s) 134
Hin1II CATG 1 cut(s) 70
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HindIII AAGCTT 1 cut(s) 200
HinfI GANTC 1 cut(s) 213
HpaII CCGG 3 cut(s) 14, 72, 92
HphI GGTGA 1 cut(s) 28
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 2 cut(s) 118, 218
Hpy188III TCNNGA 2 cut(s) 67, 79
Hpy8I GTNNAC 1 cut(s) 37
HpyAV CCTTC 2 cut(s) 19, 76
HpyF3I CTNAG 2 cut(s) 117, 177
Hsp92II CATG 1 cut(s) 70
HspAI GCGC 1 cut(s) 132
Kzo9I GATC 4 cut(s) 63, 85, 99, 126
LpnPI CCDG 7 cut(s) 27, 64, 85, 105, 154, 165, 179
Lsp1109I GCAGC 1 cut(s) 22
MaeIII GTNAC 1 cut(s) 145
MalI GATC 4 cut(s) 65, 87, 101, 128
MboI GATC 4 cut(s) 63, 85, 99, 126
MboII GAAGA 3 cut(s) 73, 109, 131
MflI RGATCY 2 cut(s) 85, 99
MhlI GDGCHC 1 cut(s) 210
MluCI AATT 2 cut(s) 20, 121
MmeI TCCRAC 1 cut(s) 139
MspI CCGG 3 cut(s) 14, 72, 92
NdeII GATC 4 cut(s) 63, 85, 99, 126
NlaIII CATG 1 cut(s) 70
NlaIV GGNNCC 1 cut(s) 163
PagI TCATGA 1 cut(s) 66
PfeI GAWTC 1 cut(s) 213
PinAI ACCGGT 1 cut(s) 71
PkrI GCNGC 1 cut(s) 12
PspN4I GGNNCC 1 cut(s) 163
PstNI CAGNNNCTG 1 cut(s) 216
PsuI RGATCY 2 cut(s) 85, 99
SatI GCNGC 1 cut(s) 11
Sau3AI GATC 4 cut(s) 63, 85, 99, 126
SduI GDGCHC 1 cut(s) 210
SetI ASST 3 cut(s) 114, 167, 204
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 2 cut(s) 20, 121
SsiI CCGC 1 cut(s) 29
TasI AATT 2 cut(s) 20, 121
TfiI GAWTC 1 cut(s) 213
TseI GCWGC 1 cut(s) 10
TspGWI ACGGA 1 cut(s) 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.