Rh4DG395500

Cytochrome b-c1 complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004343
Physical Location & Seq
Forward (+)
61208576 .. 61211521
2946 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG395500.1

Sequence Viewer

Length: 219 bp
ATGGGGAAGCAACCGGTGAAGATGAGGGCGGTGGTGTACGCTCTGTCGCCGTTTCAGCAGAAGGTGATGCCCGGCCTCTGGAAGGACCTTCCGGCCAAGCTCCACCACAAGGTCTCCGAGAACTGGCTCAGCGCCACTCTCCTCCTCGCTCCTCTCGTCGCCGTCCACACGTACGTGCAGAATTACCAGGAGAAGGAGAAGCTGGAACACAGGTACTGA

Protein Analysis

72

Amino Acids

8.38

Weight (kDa)

9.7

Isoelectric Point (pI)

52.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt_b-c1_8 PF10890 1 - 72 6.4e-36 Cytochrome b-c1 complex subunit 8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 29
AcoI YGGCCR 1 cut(s) 93
AfaI GTAC 3 cut(s) 38, 173, 215
AfiI CCNNNNNNNGG 5 cut(s) 78, 82, 109, 123, 193
AflIII ACRYGT 1 cut(s) 168
AgeI ACCGGT 1 cut(s) 13
AjnI CCWGG 1 cut(s) 186
AleI CACNNNNGTG 1 cut(s) 173
AluBI AGCT 2 cut(s) 100, 202
AluI AGCT 2 cut(s) 100, 202
Alw26I GTCTC 1 cut(s) 118
AlwNI CAGNNNCTG 1 cut(s) 216
AoxI GGCC 2 cut(s) 73, 93
AsiGI ACCGGT 1 cut(s) 13
AspLEI GCGC 1 cut(s) 134
AspS9I GGNCC 1 cut(s) 85
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 2 cut(s) 28, 76
AvaII GGWCC 1 cut(s) 85
BceAI ACGGC 2 cut(s) 34, 146
BciT130I CCWGG 1 cut(s) 188
BcnI CCSGG 1 cut(s) 72
BcoDI GTCTC 1 cut(s) 118
BfoI RGCGCY 1 cut(s) 135
BlpI GCTNAGC 1 cut(s) 128
Bme1390I CCNGG 2 cut(s) 72, 188
Bme18I GGWCC 1 cut(s) 85
BmgT120I GGNCC 1 cut(s) 85
BmrFI CCNGG 2 cut(s) 72, 188
BmsI GCATC 1 cut(s) 57
Bpu1102I GCTNAGC 1 cut(s) 128
BpuMI CCSGG 1 cut(s) 72
BsaAI YACGTR 2 cut(s) 171, 175
BsaI GGTCTC 1 cut(s) 118
BsaWI WCCGGW 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 5 cut(s) 78, 82, 109, 123, 193
Bse118I RCCGGY 1 cut(s) 13
Bse1I ACTGG 1 cut(s) 128
BseBI CCWGG 1 cut(s) 188
BseLI CCNNNNNNNGG 5 cut(s) 78, 82, 109, 123, 193
BseMII CTCAG 1 cut(s) 142
BseNI ACTGG 1 cut(s) 128
BseRI GAGGAG 3 cut(s) 131, 134, 141
BsgI GTGCAG 1 cut(s) 197
BshFI GGCC 2 cut(s) 75, 95
BshTI ACCGGT 1 cut(s) 13
BsiSI CCGG 3 cut(s) 14, 72, 92
BsiWI CGTACG 1 cut(s) 171
BslI CCNNNNNNNGG 5 cut(s) 78, 82, 109, 123, 193
BsmAI GTCTC 1 cut(s) 118
BsnI GGCC 2 cut(s) 75, 95
Bso31I GGTCTC 1 cut(s) 118
Bsp1720I GCTNAGC 1 cut(s) 128
BspACI CCGC 1 cut(s) 29
BspANI GGCC 2 cut(s) 75, 95
BspCNI CTCAG 1 cut(s) 141
BspTNI GGTCTC 1 cut(s) 118
BsrFI RCCGGY 1 cut(s) 13
BsrI ACTGG 1 cut(s) 128
BssAI RCCGGY 1 cut(s) 13
Bst2UI CCWGG 1 cut(s) 188
BstBAI YACGTR 2 cut(s) 171, 175
BstDEI CTNAG 1 cut(s) 128
BstENI CCTNNNNNAGG 1 cut(s) 80
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 1 cut(s) 134
BstMAI GTCTC 1 cut(s) 118
BstMWI GCNNNNNNNGC 1 cut(s) 55
BstNI CCWGG 1 cut(s) 188
BstSCI CCNGG 2 cut(s) 70, 186
BsuRI GGCC 2 cut(s) 75, 95
CaiI CAGNNNCTG 1 cut(s) 216
CfoI GCGC 1 cut(s) 134
Cfr10I RCCGGY 1 cut(s) 13
Cfr13I GGNCC 1 cut(s) 85
Csp6I GTAC 3 cut(s) 37, 172, 214
CspAI ACCGGT 1 cut(s) 13
CviJI RGCY 5 cut(s) 75, 95, 100, 127, 202
CviKI_1 RGCY 5 cut(s) 75, 95, 100, 127, 202
CviQI GTAC 3 cut(s) 37, 172, 214
DdeI CTNAG 1 cut(s) 128
EaeI YGGCCR 1 cut(s) 93
Eco31I GGTCTC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 85
EcoNI CCTNNNNNAGG 1 cut(s) 80
EcoO109I RGGNCCY 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 186
GlaI GCGC 1 cut(s) 133
HaeII RGCGCY 1 cut(s) 135
HaeIII GGCC 2 cut(s) 75, 95
HapII CCGG 3 cut(s) 14, 72, 92
HhaI GCGC 1 cut(s) 134
Hin6I GCGC 1 cut(s) 132
HinP1I GCGC 1 cut(s) 132
HpaII CCGG 3 cut(s) 14, 72, 92
HphI GGTGA 2 cut(s) 28, 76
Hpy166II GTNNAC 2 cut(s) 37, 166
Hpy188I TCNGA 1 cut(s) 118
Hpy188III TCNNGA 1 cut(s) 79
Hpy8I GTNNAC 2 cut(s) 37, 166
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 4 cut(s) 55, 76, 98, 187
HpyCH4IV ACGT 2 cut(s) 170, 174
HpyCH4V TGCA 1 cut(s) 178
HpyF10VI GCNNNNNNNGC 1 cut(s) 55
HpyF3I CTNAG 1 cut(s) 128
HpySE526I ACGT 2 cut(s) 170, 174
HspAI GCGC 1 cut(s) 132
LmnI GCTCC 2 cut(s) 105, 154
LpnPI CCDG 9 cut(s) 27, 64, 85, 105, 109, 173, 188, 196, 200
LweI GCATC 1 cut(s) 57
MaeII ACGT 2 cut(s) 170, 174
MboII GAAGA 1 cut(s) 31
MluCI AATT 1 cut(s) 181
MnlI CCTC 5 cut(s) 18, 86, 152, 155, 162
MslI CAYNNNNRTG 1 cut(s) 173
MspI CCGG 3 cut(s) 14, 72, 92
MspR9I CCNGG 2 cut(s) 72, 188
MvaI CCWGG 1 cut(s) 188
MwoI GCNNNNNNNGC 1 cut(s) 55
NciI CCSGG 1 cut(s) 72
OliI CACNNNNGTG 1 cut(s) 173
PcsI WCGNNNNNNNCGW 1 cut(s) 153
Pfl23II CGTACG 1 cut(s) 171
PinAI ACCGGT 1 cut(s) 13
Ppu21I YACGTR 2 cut(s) 171, 175
PpuMI RGGWCCY 1 cut(s) 85
Psp5II RGGWCCY 1 cut(s) 85
Psp6I CCWGG 1 cut(s) 186
PspGI CCWGG 1 cut(s) 186
PspLI CGTACG 1 cut(s) 171
PspPI GGNCC 1 cut(s) 85
PspPPI RGGWCCY 1 cut(s) 85
PstNI CAGNNNCTG 1 cut(s) 216
RsaI GTAC 3 cut(s) 38, 173, 215
RsaNI GTAC 3 cut(s) 37, 172, 214
RseI CAYNNNNRTG 1 cut(s) 173
Sau96I GGNCC 1 cut(s) 85
ScrFI CCNGG 2 cut(s) 72, 188
SetI ASST 8 cut(s) 66, 90, 102, 114, 173, 177, 204, 215
SfaNI GCATC 1 cut(s) 57
SinI GGWCC 1 cut(s) 85
SmiMI CAYNNNNRTG 1 cut(s) 173
Sse9I AATT 1 cut(s) 181
SsiI CCGC 1 cut(s) 29
StyD4I CCNGG 2 cut(s) 70, 186
TaiI ACGT 2 cut(s) 173, 177
TasI AATT 1 cut(s) 181
VpaK11BI GGWCC 1 cut(s) 85
XagI CCTNNNNNAGG 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.