AT5G05370

Cytochrome b-c1 complex, subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004343
Physical Location & Seq
Reverse (-)
1590797 .. 1591939
1143 bp
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UTR
Exon/CDS
Intron
AT5G05370.1

Sequence Viewer

Length: 219 bp
ATGGGAAAGCAGCCGGTGAAATTGAAGGCGGTGGTTTACGCGTTATCGCCGTTTCAGCAGAAGATCATGACGGGTCTTTGGAAGGATCTTCCGGAGAAGATTCACCACAAGGTCTCAGAGAATTGGATCAGTACTATTCTCCTCGTCGCTCCTGTCGTCGGAACCTACTCGTATGCTCAGTACTTCAAAGAACAAGAGAAGCTGGAGCACAGGTTCTAA

Protein Analysis

72

Amino Acids

8.42

Weight (kDa)

9.48

Isoelectric Point (pI)

46.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt_b-c1_8 PF10890 1 - 72 1.8e-37 Cytochrome b-c1 complex subunit 8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 41
AccIII TCCGGA 1 cut(s) 91
AciI CCGC 1 cut(s) 29
AclWI GGATC 2 cut(s) 93, 134
AfaI GTAC 2 cut(s) 133, 182
AfiI CCNNNNNNNGG 1 cut(s) 158
AflIII ACRYGT 1 cut(s) 39
AgsI TTSAA 2 cut(s) 25, 187
AloI GAACNNNNNNTCC 1 cut(s) 197
AluBI AGCT 1 cut(s) 202
AluI AGCT 1 cut(s) 202
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 2 cut(s) 93, 134
Aor13HI TCCGGA 1 cut(s) 91
ApeKI GCWGC 1 cut(s) 10
AsuHPI GGTGA 2 cut(s) 28, 95
Bbv12I GWGCWC 1 cut(s) 210
BbvI GCAGC 1 cut(s) 22
BceAI ACGGC 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 118
BisI GCNGC 1 cut(s) 11
BlsI GCNGC 1 cut(s) 12
BmcAI AGTACT 2 cut(s) 133, 182
BmiI GGNNCC 1 cut(s) 163
BsaI GGTCTC 1 cut(s) 118
BsaWI WCCGGW 1 cut(s) 91
BsaXI ACNNNNNCTCC 1 cut(s) 197
Bsc4I CCNNNNNNNGG 1 cut(s) 158
Bse118I RCCGGY 1 cut(s) 13
BseAI TCCGGA 1 cut(s) 91
BseLI CCNNNNNNNGG 1 cut(s) 158
BseMII CTCAG 2 cut(s) 129, 191
BseRI GAGGAG 1 cut(s) 131
BseXI GCAGC 1 cut(s) 22
Bsh1236I CGCG 1 cut(s) 41
BsiHKAI GWGCWC 1 cut(s) 210
BsiSI CCGG 2 cut(s) 14, 92
BslI CCNNNNNNNGG 1 cut(s) 158
BsmAI GTCTC 1 cut(s) 118
Bso31I GGTCTC 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 210
Bsp13I TCCGGA 1 cut(s) 91
Bsp143I GATC 3 cut(s) 63, 85, 126
BspACI CCGC 1 cut(s) 29
BspCNI CTCAG 2 cut(s) 128, 190
BspEI TCCGGA 1 cut(s) 91
BspFNI CGCG 1 cut(s) 41
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 1 cut(s) 163
BspPI GGATC 2 cut(s) 93, 134
BspTNI GGTCTC 1 cut(s) 118
BsrFI RCCGGY 1 cut(s) 13
BssAI RCCGGY 1 cut(s) 13
BssMI GATC 3 cut(s) 63, 85, 126
BstDEI CTNAG 2 cut(s) 115, 177
BstFNI CGCG 1 cut(s) 41
BstKTI GATC 3 cut(s) 66, 88, 129
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 3 cut(s) 63, 85, 126
BstMWI GCNNNNNNNGC 1 cut(s) 55
BstUI CGCG 1 cut(s) 41
BstV1I GCAGC 1 cut(s) 22
BstX2I RGATCY 1 cut(s) 85
BstYI RGATCY 1 cut(s) 85
CciI TCATGA 1 cut(s) 66
Cfr10I RCCGGY 1 cut(s) 13
Csp6I GTAC 2 cut(s) 132, 181
CviAII CATG 1 cut(s) 67
CviJI RGCY 2 cut(s) 13, 202
CviKI_1 RGCY 2 cut(s) 13, 202
CviQI GTAC 2 cut(s) 132, 181
DdeI CTNAG 2 cut(s) 115, 177
DpnI GATC 3 cut(s) 65, 87, 128
DpnII GATC 3 cut(s) 63, 85, 126
Eco31I GGTCTC 1 cut(s) 118
FaeI CATG 1 cut(s) 70
FaiI YATR 2 cut(s) 68, 174
FatI CATG 1 cut(s) 66
Fnu4HI GCNGC 1 cut(s) 11
Fsp4HI GCNGC 1 cut(s) 11
GluI GCNGC 1 cut(s) 11
HapII CCGG 2 cut(s) 14, 92
Hin1II CATG 1 cut(s) 70
HinfI GANTC 1 cut(s) 100
HpaII CCGG 2 cut(s) 14, 92
HphI GGTGA 2 cut(s) 28, 95
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 2 cut(s) 118, 161
Hpy188III TCNNGA 2 cut(s) 67, 92
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 2 cut(s) 149, 161
HpyAV CCTTC 2 cut(s) 19, 76
HpyF10VI GCNNNNNNNGC 1 cut(s) 55
HpyF3I CTNAG 2 cut(s) 115, 177
Hsp92II CATG 1 cut(s) 70
Kpn2I TCCGGA 1 cut(s) 91
Kzo9I GATC 3 cut(s) 63, 85, 126
LmnI GCTCC 2 cut(s) 154, 205
LpnPI CCDG 5 cut(s) 27, 105, 165, 188, 196
Lsp1109I GCAGC 1 cut(s) 22
MalI GATC 3 cut(s) 65, 87, 128
MboI GATC 3 cut(s) 63, 85, 126
MboII GAAGA 3 cut(s) 73, 80, 109
MflI RGATCY 1 cut(s) 85
MhlI GDGCHC 1 cut(s) 210
MluCI AATT 2 cut(s) 20, 121
MluI ACGCGT 1 cut(s) 39
MmeI TCCRAC 1 cut(s) 139
MnlI CCTC 1 cut(s) 152
MroI TCCGGA 1 cut(s) 91
MspI CCGG 2 cut(s) 14, 92
MvnI CGCG 1 cut(s) 41
MwoI GCNNNNNNNGC 1 cut(s) 55
NdeII GATC 3 cut(s) 63, 85, 126
NlaIII CATG 1 cut(s) 70
NlaIV GGNNCC 1 cut(s) 163
PagI TCATGA 1 cut(s) 66
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PfeI GAWTC 1 cut(s) 100
PkrI GCNGC 1 cut(s) 12
PspN4I GGNNCC 1 cut(s) 163
PsuI RGATCY 1 cut(s) 85
RsaI GTAC 2 cut(s) 133, 182
RsaNI GTAC 2 cut(s) 132, 181
SatI GCNGC 1 cut(s) 11
Sau3AI GATC 3 cut(s) 63, 85, 126
ScaI AGTACT 2 cut(s) 133, 182
SduI GDGCHC 1 cut(s) 210
SetI ASST 4 cut(s) 114, 167, 204, 215
Sse9I AATT 2 cut(s) 20, 121
SsiI CCGC 1 cut(s) 29
TasI AATT 2 cut(s) 20, 121
TatI WGTACW 2 cut(s) 131, 180
TfiI GAWTC 1 cut(s) 100
TseI GCWGC 1 cut(s) 10
ZrmI AGTACT 2 cut(s) 133, 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.