MD16G1095600.v1.1

Cytochrome b-c1 complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004343
Physical Location & Seq
Forward (+)
6648941 .. 6649698
758 bp
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UTR
Exon/CDS
Intron
MD16G1095600.v1.1.491

Sequence Viewer

Length: 219 bp
ATGGGGAAGATTCCGGTGAAGATGAGAGCAGTGGTGTACGCGCTGTCGCCGTTTCAGCAGAAGGTGATGCCCGGCCTCTGGAAGGACTTCACCGGCAAGATCCACCACAAGGTCTCCGAGAACTGGCTCAGCGCCACCTTCCTCCTCACTCCTCTCGTCGCCACCTACTCGTACGTGCAGTATTACCAGGAGCAGGAGAAGTTGGAACACAGGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt_b-c1_8

Protein Analysis

73

Amino Acids

8.52

Weight (kDa)

9.48

Isoelectric Point (pI)

44.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt_b-c1_8 PF10890 1 - 72 1e-36 Cytochrome b-c1 complex subunit 8
UcrQ PF02939 10 - 66 3.6e-06 UcrQ family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 41
AclWI GGATC 1 cut(s) 94
AfaI GTAC 3 cut(s) 38, 173, 215
AfiI CCNNNNNNNGG 5 cut(s) 78, 82, 109, 123, 193
AjnI CCWGG 1 cut(s) 186
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 1 cut(s) 94
AlwNI CAGNNNCTG 1 cut(s) 216
AoxI GGCC 1 cut(s) 73
Asp700I GAANNNNTTC 1 cut(s) 86
AspLEI GCGC 2 cut(s) 43, 134
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 3 cut(s) 28, 76, 82
BceAI ACGGC 1 cut(s) 34
BciT130I CCWGG 1 cut(s) 188
BcnI CCSGG 1 cut(s) 72
BcoDI GTCTC 1 cut(s) 118
BfoI RGCGCY 1 cut(s) 135
BlpI GCTNAGC 1 cut(s) 128
Bme1390I CCNGG 2 cut(s) 72, 188
BmrFI CCNGG 2 cut(s) 72, 188
BmsI GCATC 1 cut(s) 57
Bpu1102I GCTNAGC 1 cut(s) 128
BpuMI CCSGG 1 cut(s) 72
BsaAI YACGTR 1 cut(s) 175
BsaI GGTCTC 1 cut(s) 118
BsaWI WCCGGW 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 5 cut(s) 78, 82, 109, 123, 193
Bse118I RCCGGY 1 cut(s) 92
Bse1I ACTGG 1 cut(s) 128
BseBI CCWGG 1 cut(s) 188
BseLI CCNNNNNNNGG 5 cut(s) 78, 82, 109, 123, 193
BseMII CTCAG 1 cut(s) 142
BseNI ACTGG 1 cut(s) 128
BseRI GAGGAG 2 cut(s) 134, 141
BsgI GTGCAG 1 cut(s) 197
Bsh1236I CGCG 1 cut(s) 41
BshFI GGCC 1 cut(s) 75
BsiSI CCGG 3 cut(s) 14, 72, 93
BsiWI CGTACG 1 cut(s) 171
BslI CCNNNNNNNGG 5 cut(s) 78, 82, 109, 123, 193
BsmAI GTCTC 1 cut(s) 118
BsnI GGCC 1 cut(s) 75
Bso31I GGTCTC 1 cut(s) 118
Bsp143I GATC 1 cut(s) 99
Bsp1720I GCTNAGC 1 cut(s) 128
BspANI GGCC 1 cut(s) 75
BspCNI CTCAG 1 cut(s) 141
BspFNI CGCG 1 cut(s) 41
BspPI GGATC 1 cut(s) 94
BspTNI GGTCTC 1 cut(s) 118
BsrFI RCCGGY 1 cut(s) 92
BsrI ACTGG 1 cut(s) 128
BssAI RCCGGY 1 cut(s) 92
BssMI GATC 1 cut(s) 99
Bst2UI CCWGG 1 cut(s) 188
BstBAI YACGTR 1 cut(s) 175
BstDEI CTNAG 1 cut(s) 128
BstENI CCTNNNNNAGG 1 cut(s) 80
BstFNI CGCG 1 cut(s) 41
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 2 cut(s) 43, 134
BstKTI GATC 1 cut(s) 102
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 1 cut(s) 99
BstMWI GCNNNNNNNGC 1 cut(s) 55
BstNI CCWGG 1 cut(s) 188
BstSCI CCNGG 2 cut(s) 70, 186
BstUI CGCG 1 cut(s) 41
BstX2I RGATCY 1 cut(s) 99
BstYI RGATCY 1 cut(s) 99
BsuRI GGCC 1 cut(s) 75
BtsI GCAGTG 1 cut(s) 36
BtsIMutI CAGTG 1 cut(s) 36
CaiI CAGNNNCTG 1 cut(s) 216
CfoI GCGC 2 cut(s) 43, 134
Cfr10I RCCGGY 1 cut(s) 92
Csp6I GTAC 3 cut(s) 37, 172, 214
CviJI RGCY 2 cut(s) 75, 127
CviKI_1 RGCY 2 cut(s) 75, 127
CviQI GTAC 3 cut(s) 37, 172, 214
DdeI CTNAG 1 cut(s) 128
DpnI GATC 1 cut(s) 101
DpnII GATC 1 cut(s) 99
Eco31I GGTCTC 1 cut(s) 118
EcoNI CCTNNNNNAGG 1 cut(s) 80
EcoRII CCWGG 1 cut(s) 186
GlaI GCGC 2 cut(s) 42, 133
HaeII RGCGCY 1 cut(s) 135
HaeIII GGCC 1 cut(s) 75
HapII CCGG 3 cut(s) 14, 72, 93
HhaI GCGC 2 cut(s) 43, 134
Hin6I GCGC 2 cut(s) 41, 132
HinP1I GCGC 2 cut(s) 41, 132
HinfI GANTC 1 cut(s) 10
HpaII CCGG 3 cut(s) 14, 72, 93
HphI GGTGA 3 cut(s) 28, 76, 82
Hpy166II GTNNAC 1 cut(s) 37
Hpy188I TCNGA 1 cut(s) 118
Hpy188III TCNNGA 1 cut(s) 79
Hpy8I GTNNAC 1 cut(s) 37
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 3 cut(s) 55, 76, 148
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 1 cut(s) 178
HpyF10VI GCNNNNNNNGC 1 cut(s) 55
HpyF3I CTNAG 1 cut(s) 128
HpySE526I ACGT 1 cut(s) 174
HspAI GCGC 2 cut(s) 41, 132
Kzo9I GATC 1 cut(s) 99
LmnI GCTCC 1 cut(s) 190
LpnPI CCDG 9 cut(s) 27, 64, 85, 106, 109, 173, 179, 196, 200
LweI GCATC 1 cut(s) 57
MaeII ACGT 1 cut(s) 174
MalI GATC 1 cut(s) 101
MboI GATC 1 cut(s) 99
MboII GAAGA 2 cut(s) 19, 31
MflI RGATCY 1 cut(s) 99
MmeI TCCRAC 1 cut(s) 183
MnlI CCTC 4 cut(s) 86, 152, 155, 162
MroXI GAANNNNTTC 1 cut(s) 86
MspI CCGG 3 cut(s) 14, 72, 93
MspR9I CCNGG 2 cut(s) 72, 188
MvaI CCWGG 1 cut(s) 188
MvnI CGCG 1 cut(s) 41
MwoI GCNNNNNNNGC 1 cut(s) 55
NciI CCSGG 1 cut(s) 72
NdeII GATC 1 cut(s) 99
PdmI GAANNNNTTC 1 cut(s) 86
PfeI GAWTC 1 cut(s) 10
Pfl23II CGTACG 1 cut(s) 171
Ppu21I YACGTR 1 cut(s) 175
Psp6I CCWGG 1 cut(s) 186
PspGI CCWGG 1 cut(s) 186
PspLI CGTACG 1 cut(s) 171
PstNI CAGNNNCTG 1 cut(s) 216
PsuI RGATCY 1 cut(s) 99
RsaI GTAC 3 cut(s) 38, 173, 215
RsaNI GTAC 3 cut(s) 37, 172, 214
Sau3AI GATC 1 cut(s) 99
ScrFI CCNGG 2 cut(s) 72, 188
SetI ASST 6 cut(s) 66, 114, 140, 167, 177, 215
SfaNI GCATC 1 cut(s) 57
StyD4I CCNGG 2 cut(s) 70, 186
TaiI ACGT 1 cut(s) 177
TfiI GAWTC 1 cut(s) 10
TscAI CASTG 1 cut(s) 36
TspRI CASTG 1 cut(s) 36
XagI CCTNNNNNAGG 1 cut(s) 80
XmnI GAANNNNTTC 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.