Rh5AG483300

Cytochrome b-c1 complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004343
Physical Location & Seq
Reverse (-)
82707943 .. 82710413
2471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG483300.1

Sequence Viewer

Length: 417 bp
ATGGGGAAGCAACCGGTGAGGATGAAGGCGGTGGTATACGCGCTCTCGCCGTTTCAGCAGAAGGTGATGCCGGGTCTCTGGAAAGATCTGCCGACGAAGATCCACCACAAGATCTCCGAGAACTGGCACAGCGCTATCTTCCTCCTCGGACCCATCCTCGGAACCTATGCTTGCGTCAACAAATGGAAAATTCCAGCTTTGAGGATGCTGCATGAGAGCAGATTCAGGAGATTGTTAGAAGGAGGGTGTCGCCTTTGTTTAGCTCAAAGGAGATTTAGATTGGGATATGCATCAGTTATCCAACGTAGCTTGTTTCAGGACTGCTACAGGGTCTCTAATCTCTATGTAGTTCTTACTTGTTGTAGTACCTCATCATTTTCTGGGAATATGGTTCCCAGATATCTCATTTTCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyt_b-c1_8

Protein Analysis

138

Amino Acids

15.97

Weight (kDa)

10.15

Isoelectric Point (pI)

58.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt_b-c1_8 PF10890 1 - 59 5.6e-28 Cytochrome b-c1 complex subunit 8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 36
AccII CGCG 1 cut(s) 41
AciI CCGC 1 cut(s) 29
AclWI GGATC 1 cut(s) 94
AcsI RAATTY 1 cut(s) 189
AfaI GTAC 1 cut(s) 367
AfeI AGCGCT 1 cut(s) 133
AfiI CCNNNNNNNGG 2 cut(s) 123, 158
AgeI ACCGGT 1 cut(s) 13
AluBI AGCT 3 cut(s) 197, 263, 309
AluI AGCT 3 cut(s) 197, 263, 309
Alw26I GTCTC 2 cut(s) 80, 337
AlwI GGATC 1 cut(s) 94
Aor51HI AGCGCT 1 cut(s) 133
ApeKI GCWGC 1 cut(s) 208
ApoI RAATTY 1 cut(s) 189
AsiGI ACCGGT 1 cut(s) 13
AspLEI GCGC 2 cut(s) 43, 134
AspS9I GGNCC 1 cut(s) 149
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 2 cut(s) 28, 76
AvaII GGWCC 1 cut(s) 149
BbvI GCAGC 1 cut(s) 195
BccI CCATC 1 cut(s) 161
BceAI ACGGC 1 cut(s) 34
BcnI CCSGG 1 cut(s) 72
BcoDI GTCTC 2 cut(s) 80, 337
BfmI CTRYAG 1 cut(s) 325
BfoI RGCGCY 1 cut(s) 135
BglII AGATCT 2 cut(s) 85, 111
BisI GCNGC 1 cut(s) 209
BlsI GCNGC 1 cut(s) 210
Bme1390I CCNGG 1 cut(s) 72
Bme18I GGWCC 1 cut(s) 149
BmgT120I GGNCC 1 cut(s) 149
BmiI GGNNCC 3 cut(s) 151, 163, 393
BmrFI CCNGG 1 cut(s) 72
BmsI GCATC 3 cut(s) 57, 195, 299
BpuMI CCSGG 1 cut(s) 72
BsaBI GATNNNNATC 1 cut(s) 289
BsaI GGTCTC 2 cut(s) 80, 337
BsaJI CCNNGG 2 cut(s) 145, 157
BsaWI WCCGGW 1 cut(s) 13
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 158
Bse118I RCCGGY 1 cut(s) 13
Bse1I ACTGG 1 cut(s) 128
Bse8I GATNNNNATC 1 cut(s) 289
BseDI CCNNGG 2 cut(s) 145, 157
BseGI GGATG 3 cut(s) 27, 153, 210
BseJI GATNNNNATC 1 cut(s) 289
BseLI CCNNNNNNNGG 2 cut(s) 123, 158
BseNI ACTGG 1 cut(s) 128
BseRI GAGGAG 1 cut(s) 134
BseXI GCAGC 1 cut(s) 195
Bsh1236I CGCG 1 cut(s) 41
BshTI ACCGGT 1 cut(s) 13
BsiSI CCGG 2 cut(s) 14, 71
BslI CCNNNNNNNGG 2 cut(s) 123, 158
BsmAI GTCTC 2 cut(s) 80, 337
Bso31I GGTCTC 2 cut(s) 80, 337
Bsp143I GATC 3 cut(s) 85, 99, 111
BspACI CCGC 1 cut(s) 29
BspFNI CGCG 1 cut(s) 41
BspLI GGNNCC 3 cut(s) 151, 163, 393
BspPI GGATC 1 cut(s) 94
BspTNI GGTCTC 2 cut(s) 80, 337
BsrFI RCCGGY 1 cut(s) 13
BsrI ACTGG 1 cut(s) 128
BssAI RCCGGY 1 cut(s) 13
BssECI CCNNGG 2 cut(s) 145, 157
BssMI GATC 3 cut(s) 85, 99, 111
BssNAI GTATAC 1 cut(s) 37
Bst1107I GTATAC 1 cut(s) 37
BstC8I GCNNGC 1 cut(s) 172
BstF5I GGATG 3 cut(s) 27, 153, 210
BstFNI CGCG 1 cut(s) 41
BstH2I RGCGCY 1 cut(s) 135
BstHHI GCGC 2 cut(s) 43, 134
BstKTI GATC 3 cut(s) 88, 102, 114
BstMAI GTCTC 2 cut(s) 80, 337
BstMBI GATC 3 cut(s) 85, 99, 111
BstMWI GCNNNNNNNGC 1 cut(s) 55
BstSCI CCNGG 1 cut(s) 70
BstSFI CTRYAG 1 cut(s) 325
BstUI CGCG 1 cut(s) 41
BstV1I GCAGC 1 cut(s) 195
BstX2I RGATCY 3 cut(s) 85, 99, 111
BstYI RGATCY 3 cut(s) 85, 99, 111
BstZ17I GTATAC 1 cut(s) 37
BtsCI GGATG 3 cut(s) 27, 153, 210
Cac8I GCNNGC 1 cut(s) 172
CfoI GCGC 2 cut(s) 43, 134
Cfr10I RCCGGY 1 cut(s) 13
Cfr13I GGNCC 1 cut(s) 149
CseI GACGC 1 cut(s) 163
Csp6I GTAC 1 cut(s) 366
CspAI ACCGGT 1 cut(s) 13
CviAII CATG 1 cut(s) 212
CviJI RGCY 3 cut(s) 197, 263, 309
CviKI_1 RGCY 3 cut(s) 197, 263, 309
CviQI GTAC 1 cut(s) 366
DpnI GATC 3 cut(s) 87, 101, 113
DpnII GATC 3 cut(s) 85, 99, 111
Eco31I GGTCTC 2 cut(s) 80, 337
Eco32I GATATC 1 cut(s) 401
Eco47I GGWCC 1 cut(s) 149
Eco47III AGCGCT 1 cut(s) 133
EcoRV GATATC 1 cut(s) 401
EcoT22I ATGCAT 1 cut(s) 292
FaeI CATG 1 cut(s) 215
FaiI YATR 7 cut(s) 37, 168, 213, 288, 345, 389, 415
FatI CATG 1 cut(s) 211
FblI GTMKAC 1 cut(s) 36
Fnu4HI GCNGC 1 cut(s) 209
FokI GGATG 3 cut(s) 34, 140, 217
Fsp4HI GCNGC 1 cut(s) 209
GlaI GCGC 2 cut(s) 42, 133
GluI GCNGC 1 cut(s) 209
HaeII RGCGCY 1 cut(s) 135
HapII CCGG 2 cut(s) 14, 71
HgaI GACGC 1 cut(s) 163
HhaI GCGC 2 cut(s) 43, 134
Hin1II CATG 1 cut(s) 215
Hin6I GCGC 2 cut(s) 41, 132
HinP1I GCGC 2 cut(s) 41, 132
HincII GTYRAC 1 cut(s) 178
HindII GTYRAC 1 cut(s) 178
HinfI GANTC 1 cut(s) 222
HpaII CCGG 2 cut(s) 14, 71
HphI GGTGA 2 cut(s) 28, 76
Hpy166II GTNNAC 2 cut(s) 37, 178
Hpy188I TCNGA 3 cut(s) 118, 149, 161
Hpy188III TCNNGA 3 cut(s) 79, 226, 317
Hpy8I GTNNAC 2 cut(s) 37, 178
Hpy99I CGWCG 1 cut(s) 97
HpyAV CCTTC 3 cut(s) 19, 55, 233
HpyCH4IV ACGT 1 cut(s) 304
HpyCH4V TGCA 2 cut(s) 211, 290
HpyF10VI GCNNNNNNNGC 1 cut(s) 55
HpySE526I ACGT 1 cut(s) 304
Hsp92II CATG 1 cut(s) 215
HspAI GCGC 2 cut(s) 41, 132
Kzo9I GATC 3 cut(s) 85, 99, 111
Lsp1109I GCAGC 1 cut(s) 195
LweI GCATC 3 cut(s) 57, 195, 299
MaeII ACGT 1 cut(s) 304
MalI GATC 3 cut(s) 87, 101, 113
MboI GATC 3 cut(s) 85, 99, 111
MboII GAAGA 2 cut(s) 109, 130
MflI RGATCY 3 cut(s) 85, 99, 111
MluCI AATT 1 cut(s) 189
MmeI TCCRAC 1 cut(s) 325
MnlI CCTC 7 cut(s) 12, 152, 155, 167, 195, 236, 379
Mph1103I ATGCAT 1 cut(s) 292
MspI CCGG 2 cut(s) 14, 71
MspR9I CCNGG 1 cut(s) 72
MvnI CGCG 1 cut(s) 41
MwoI GCNNNNNNNGC 1 cut(s) 55
NciI CCSGG 1 cut(s) 72
NdeII GATC 3 cut(s) 85, 99, 111
NlaIII CATG 1 cut(s) 215
NlaIV GGNNCC 3 cut(s) 151, 163, 393
NsiI ATGCAT 1 cut(s) 292
PfeI GAWTC 1 cut(s) 222
PinAI ACCGGT 1 cut(s) 13
PkrI GCNGC 1 cut(s) 210
PspN4I GGNNCC 3 cut(s) 151, 163, 393
PspPI GGNCC 1 cut(s) 149
PsuI RGATCY 3 cut(s) 85, 99, 111
RsaI GTAC 1 cut(s) 367
RsaNI GTAC 1 cut(s) 366
SatI GCNGC 1 cut(s) 209
Sau3AI GATC 3 cut(s) 85, 99, 111
Sau96I GGNCC 1 cut(s) 149
ScrFI CCNGG 1 cut(s) 72
SetI ASST 7 cut(s) 66, 167, 199, 265, 307, 311, 371
SfaNI GCATC 3 cut(s) 57, 195, 299
SfcI CTRYAG 1 cut(s) 325
SinI GGWCC 1 cut(s) 149
Sse9I AATT 1 cut(s) 189
SsiI CCGC 1 cut(s) 29
StyD4I CCNGG 1 cut(s) 70
TaiI ACGT 1 cut(s) 307
TasI AATT 1 cut(s) 189
TfiI GAWTC 1 cut(s) 222
TseI GCWGC 1 cut(s) 208
TspDTI ATGAA 1 cut(s) 38
VpaK11BI GGWCC 1 cut(s) 149
XapI RAATTY 1 cut(s) 189
XmiI GTMKAC 1 cut(s) 36
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.