AT3G12915

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001995
Physical Location & Seq
Forward (+)
4112834 .. 4115951
3118 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G12915.2

Sequence Viewer

Length: 2367 bp
ATGGTGAAGTTTACAGTTGAAGAGCAACGGAAAATTACGGATCGTAAACACAACATCCGTAACATGTCTGTCATCGCCCATGTCGATCATGGGAAATCTACACTTACGGATTCACTTGTTGCTGCTGCTGGTATCATTGCTCAAGAAACTGCGGGTGATGTGCGTATGACTGATACTCGTGCTGATGAGGCAGAACGTGGTATCACCATCAAGTCCACTGGTATCTCTCTCTACTATGAGATGACTGATGCGTCTCTGAAGAGTTTCACCGGAGCTAGAGATGGCAATGAGTACCTGATCAACCTCATTGACTCTCCTGGACACGTTGACTTTTCTTCAGAGGTCACAGCTGCTCTCCGTATCACTGATGGTGCTCTTGTGGTTGTTGACTGCATTGAAGGTGTTTGTGTGCAGACTGAGACTGTGCTCAGGCAATCTCTTGGTGAAAGGATTAGGCCTGTTTTGACTGTCAACAAGATGGACAGGTGTTTCCTTGAGCTTAAGGTTGATGGTGAGGAGGCGTATCAGAATTTCCAGAGGGTCATTGAGAATGCTAATGTCATCATGGCCACGCATGAGGATCCTCTCCTCGGTGATGTTCAGGTTTACCCTGAAAAGGGGACAGTAGCTTTCTCTGCTGGTCTCCACGGATGGGCTTTCACTCTCACCAATTTTGCTAAGATGTATGCTTCCAAGTTTGGTGTCTCTGAATCTAAAATGATGGAGAGGCTGTGGGGTGAGAACTTCTTTGATTCTGCAACCAGAAAATGGACGACCAAAAACTGGCTCCCCGACCTGCAAGCGTGGAAGGATAAGCTGTGGCCGATGTTAGAGAAGCTTGGTATTCAGATGAAGCCCGATGAGAAAGAGTTGATGGGTAAACCTTTGATGAAGCGTGTCATGCAGGCATGGCTTCCTGCGAGTACCGCTCTTCTTGAAATGATGATCTTTCACTTACCATCACCCTACACTGCGCAGAGGTACCGTGTTGAGAACTTGTATGAAGGTCCCCTTGATGATAAGTATGCTGCAGCTATCAGAAACTGTGATCCAGACGGTCCTTTGATGCTCTACGTCTCTAAGATGATTCCCGCATCTGACAAGGGCAGGTTCTTTGCCTTCGGTCGTGTCTTTTCTGGTACGGTTTCTACTGGTATGAAAGTCAGGATCATGGGTCCTAACTATGTCCCTGGTGAAAAGAAAGATCTGTATGTCAAAAGTGTGCAGAGGACTGTCATTTGGATGGGTAAGAAACAAGAGACAGTGGAGGATGTTCCTTGTGGTAATACTGTTGCTATGGTTGGGCTGGATCAGTTCATCACCAAGAATGCGTCTGACCTTCCCAAGCTTGTAGAAGGTCTTAAGAGGCTGGCCAAGTCTGACCCTATGGTTCTATGCACAATGGAAGAGTCTGGTGAGCACATTGTTGCAGGTGCTGGAGAACTCCACATTGAAATTTGTGTCAAGGATCTTCAAGATTTTATGGGTGGTGCGGATATCATAGTGTCAGATCCTGTTGTTTCGTTACGAGAGACAGTCTTTGAGAGGTCTTGCCGGACTGTGATGAGTAAGTCCCCAAACAAACACAACCGTCTTTACATGGAAGCCAGACCAATGGAGGATGGTCTAGCAGAGGCCATTGATGAAGGCCGCATCGGTCCATCTGATGACCCCAAGATCCGATCCAAGATTCTTGCAGAAGAGTTTGGGTGGGACAAGGATCTTGCCAAGAAAATCTGGGCGTTTGGACCTGACACCACAGGGCCCAACATGGTGGTTGACATGTGTAAGGGAGTGCAGTACTTGAATGAAATCAAGGACTCAGTGGTTGCTGGGTTCCAATGGGCTTCAAAGGAAGGTCCTTTAGCAGAGGAAAACATGAGAGGGGTCTGTTATGAGGTGTGTGATGTTGTGCTCCATGCTGATGCAATCCACAGAGGATGTGGTCAGATGATCTCAACAGCAAGAAGAGCCATCTATGCATCACAACTCACGGCCAAGCCTAGACTTCTTGAGCCGGTTTACATGGTCGAGATACAAGCACCTGAGGGAGCTTTAGGAGGAATCTACAGTGTGTTGAATCAGAAGCGTGGACATGTGTTTGAAGAAATGCAAAGGCCAGGAACGCCTCTCTACAACATCAAAGCCTACCTTCCTGTGGTCGAGTCATTTGGTTTCTCAGGTCAGCTAAGGGCTGCAACCTCAGGACAAGCCTTTCCTCAGTGTGTGTTTGACCATTGGGACATGATGTCTTCTGATCCGCTTGAGACTGGCTCACAGGCTGCGACATTGGTAGCTGATATCAGGAAGAGAAAAGGTTTGAAGCTACAAATGACTCCACTCTCTGATTATGAAGACAAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04152 ko04921 map04152 map04921
Pfam Domains
Protein Families
EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

788

Amino Acids

87.83

Weight (kDa)

5.65

Isoelectric Point (pI)

33.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 18 - 254 1.8e-48 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 358 - 441 1.3e-06 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 373 - 439 7.4e-12 Elongation factor Tu domain 2
EFG_III PF14492 442 - 493 8.6e-08 Elongation Factor G, domain III
EFG_IV PF03764 556 - 667 5.2e-28 Elongation factor G, domain IV
EFG_C PF00679 669 - 756 3.2e-20 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1421
AasI GACNNNNNNGTC 1 cut(s) 250
Acc16I TGCGCA 1 cut(s) 975
Acc36I ACCTGC 3 cut(s) 804, 1098, 1421
Acc65I GGTACC 1 cut(s) 981
AccB1I GGYRCC 1 cut(s) 981
AccB7I CCANNNNNTGG 2 cut(s) 768, 783
AccBSI CCGCTC 1 cut(s) 929
AciI CCGC 6 cut(s) 152, 927, 1092, 1493, 1651, 2261
AcoI YGGCCR 4 cut(s) 567, 821, 1371, 1995
AcsI RAATTY 2 cut(s) 529, 1455
AcuI CTGAAG 2 cut(s) 278, 321
AfaI GTAC 5 cut(s) 293, 925, 983, 1141, 1802
AfiI CCNNNNNNNGG 7 cut(s) 590, 616, 617, 652, 768, 783, 2158
AflII CTTAAG 2 cut(s) 500, 1361
AflIII ACRYGT 4 cut(s) 63, 322, 1782, 2095
AhdI GACNNNNNGTC 1 cut(s) 2248
AjnI CCWGG 3 cut(s) 316, 1189, 2119
Alw21I GWGCWC 4 cut(s) 376, 429, 1422, 1917
Alw26I GTCTC 8 cut(s) 258, 413, 647, 709, 1081, 1253, 1526, 2261
AlwNI CAGNNNCTG 3 cut(s) 1044, 1436, 1514
AoxI GGCC 9 cut(s) 455, 567, 821, 1371, 1635, 1648, 1763, 1995, 2117
ApaI GGGCCC 1 cut(s) 1767
ApeKI GCWGC 7 cut(s) 122, 125, 350, 1028, 1031, 2195, 2282
ApoI RAATTY 2 cut(s) 529, 1455
Asp700I GAANNNNTTC 1 cut(s) 263
Asp718I GGTACC 1 cut(s) 981
AspLEI GCGC 1 cut(s) 976
AspS9I GGNCC 8 cut(s) 1007, 1058, 1175, 1658, 1748, 1763, 1764, 1859
AvaII GGWCC 6 cut(s) 1007, 1058, 1175, 1658, 1748, 1859
AxyI CCTNAGG 2 cut(s) 2046, 2203
BaeGI GKGCMC 1 cut(s) 1767
BalI TGGCCA 2 cut(s) 569, 1373
BamHI GGATCC 1 cut(s) 580
BanI GGYRCC 1 cut(s) 981
BanII GRGCYC 1 cut(s) 1767
BauI CACGAG 1 cut(s) 177
BbsI GAAGAC 2 cut(s) 2244, 2361
Bbv12I GWGCWC 4 cut(s) 376, 429, 1422, 1917
BbvI GCAGC 7 cut(s) 109, 112, 337, 1015, 1043, 2182, 2269
BceAI ACGGC 1 cut(s) 2010
BciT130I CCWGG 3 cut(s) 318, 1191, 2121
BclI TGATCA 1 cut(s) 297
BcoDI GTCTC 8 cut(s) 258, 413, 647, 709, 1081, 1253, 1526, 2261
BfaI CTAG 3 cut(s) 276, 1628, 2004
BfmI CTRYAG 2 cut(s) 1029, 2068
BfrI CTTAAG 2 cut(s) 500, 1361
BfuAI ACCTGC 3 cut(s) 804, 1098, 1421
BglII AGATCT 1 cut(s) 1204
BisI GCNGC 8 cut(s) 123, 126, 351, 1029, 1032, 1651, 2196, 2283
BlsI GCNGC 8 cut(s) 124, 127, 352, 1030, 1033, 1652, 2197, 2284
BmcAI AGTACT 1 cut(s) 1802
Bme1390I CCNGG 3 cut(s) 318, 1191, 2121
Bme18I GGWCC 6 cut(s) 1007, 1058, 1175, 1658, 1748, 1859
BmeRI GACNNNNNGTC 1 cut(s) 2248
BmgT120I GGNCC 8 cut(s) 1007, 1058, 1175, 1658, 1748, 1763, 1764, 1859
BmiI GGNNCC 7 cut(s) 582, 788, 983, 1009, 1176, 1765, 1838
BmrFI CCNGG 3 cut(s) 318, 1191, 2121
BmsI GCATC 6 cut(s) 238, 1056, 1103, 1662, 1915, 1991
BpiI GAAGAC 2 cut(s) 2244, 2361
BplI GAGNNNNNCTC 4 cut(s) 913, 945, 2258, 2290
BpmI CTGGAG 1 cut(s) 1458
Bpu10I CCTNAGC 2 cut(s) 428, 2189
BpuEI CTTGAG 4 cut(s) 126, 515, 2033, 2285
BsaI GGTCTC 1 cut(s) 647
BsaJI CCNNGG 3 cut(s) 589, 646, 1189
BsaWI WCCGGW 1 cut(s) 269
BsaXI ACNNNNNCTCC 2 cut(s) 716, 746
Bsc4I CCNNNNNNNGG 7 cut(s) 590, 616, 617, 652, 768, 783, 2158
Bse118I RCCGGY 1 cut(s) 2017
Bse1I ACTGG 4 cut(s) 223, 788, 1156, 2275
Bse21I CCTNAGG 2 cut(s) 2046, 2203
Bse3DI GCAATG 2 cut(s) 135, 292
BseBI CCWGG 3 cut(s) 318, 1191, 2121
BseDI CCNNGG 3 cut(s) 589, 646, 1189
BseGI GGATG 6 cut(s) 54, 656, 1248, 1276, 1627, 1946
BseLI CCNNNNNNNGG 7 cut(s) 590, 616, 617, 652, 768, 783, 2158
BseMI GCAATG 2 cut(s) 135, 292
BseMII CTCAG 7 cut(s) 408, 442, 1836, 2037, 2193, 2217, 2234
BseNI ACTGG 4 cut(s) 223, 788, 1156, 2275
BseRI GAGGAG 2 cut(s) 530, 578
BseSI GKGCMC 1 cut(s) 1767
BseXI GCAGC 7 cut(s) 109, 112, 337, 1015, 1043, 2182, 2269
BseYI CCCAGC 1 cut(s) 1832
BsgI GTGCAG 3 cut(s) 431, 1244, 1817
Bsh1285I CGRYCG 1 cut(s) 1126
BshFI GGCC 9 cut(s) 457, 569, 823, 1373, 1637, 1650, 1765, 1997, 2119
BshNI GGYRCC 1 cut(s) 981
BsiEI CGRYCG 1 cut(s) 1126
BsiHKAI GWGCWC 4 cut(s) 376, 429, 1422, 1917
BsiSI CCGG 3 cut(s) 270, 1555, 2018
BslFI GGGAC 6 cut(s) 634, 993, 1172, 1558, 1727, 2255
BslI CCNNNNNNNGG 7 cut(s) 590, 616, 617, 652, 768, 783, 2158
BsmAI GTCTC 8 cut(s) 258, 413, 647, 709, 1081, 1253, 1526, 2261
BsmBI CGTCTC 2 cut(s) 258, 1081
BsmFI GGGAC 6 cut(s) 634, 993, 1172, 1558, 1727, 2255
BsmI GAATGC 2 cut(s) 556, 1333
BsnI GGCC 9 cut(s) 457, 569, 823, 1373, 1637, 1650, 1765, 1997, 2119
Bso31I GGTCTC 1 cut(s) 647
Bsp120I GGGCCC 1 cut(s) 1763
Bsp1286I GDGCHC 5 cut(s) 376, 429, 1422, 1767, 1917
BspACI CCGC 6 cut(s) 152, 927, 1092, 1493, 1651, 2261
BspANI GGCC 9 cut(s) 457, 569, 823, 1373, 1637, 1650, 1765, 1997, 2119
BspCNI CTCAG 7 cut(s) 409, 441, 1835, 2038, 2192, 2216, 2233
BspLI GGNNCC 7 cut(s) 582, 788, 983, 1009, 1176, 1765, 1838
BspMAI CTGCAG 1 cut(s) 1033
BspMI ACCTGC 3 cut(s) 804, 1098, 1421
BspQI GCTCTTC 3 cut(s) 15, 936, 1963
BspT107I GGYRCC 1 cut(s) 981
BspTI CTTAAG 2 cut(s) 500, 1361
BspTNI GGTCTC 1 cut(s) 647
BsrBI CCGCTC 1 cut(s) 929
BsrDI GCAATG 2 cut(s) 135, 292
BsrFI RCCGGY 1 cut(s) 2017
BsrI ACTGG 4 cut(s) 223, 788, 1156, 2275
BssAI RCCGGY 1 cut(s) 2017
BssECI CCNNGG 3 cut(s) 589, 646, 1189
BssSI CACGAG 1 cut(s) 177
Bst2BI CACGAG 1 cut(s) 177
Bst2UI CCWGG 3 cut(s) 318, 1191, 2121
Bst6I CTCTTC 7 cut(s) 15, 254, 936, 1401, 1695, 1963, 2303
BstAFI CTTAAG 2 cut(s) 500, 1361
BstC8I GCNNGC 3 cut(s) 801, 906, 1371
BstDSI CCRYGG 1 cut(s) 646
BstF5I GGATG 6 cut(s) 54, 656, 1248, 1276, 1627, 1946
BstHHI GCGC 1 cut(s) 976
BstMAI GTCTC 8 cut(s) 258, 413, 647, 709, 1081, 1253, 1526, 2261
BstMCI CGRYCG 1 cut(s) 1126
BstMWI GCNNNNNNNGC 8 cut(s) 188, 635, 901, 910, 926, 1970, 1979, 2125
BstNI CCWGG 3 cut(s) 318, 1191, 2121
BstNSI RCATGY 3 cut(s) 67, 1786, 2099
BstSCI CCNGG 3 cut(s) 316, 1189, 2119
BstSFI CTRYAG 2 cut(s) 1029, 2068
BstSLI GKGCMC 1 cut(s) 1767
BstV1I GCAGC 7 cut(s) 109, 112, 337, 1015, 1043, 2182, 2269
BstV2I GAAGAC 2 cut(s) 2244, 2361
BstX2I RGATCY 6 cut(s) 580, 1204, 1468, 1510, 1677, 1720
BstXI CCANNNNNNTGG 2 cut(s) 1615, 1774
BstYI RGATCY 6 cut(s) 580, 1204, 1468, 1510, 1677, 1720
Bsu36I CCTNAGG 2 cut(s) 2046, 2203
BsuRI GGCC 9 cut(s) 457, 569, 823, 1373, 1637, 1650, 1765, 1997, 2119
BtgI CCRYGG 1 cut(s) 646
BtgZI GCGATG 1 cut(s) 58
BtsCI GGATG 6 cut(s) 54, 656, 1248, 1276, 1627, 1946
BtsI GCAGTG 1 cut(s) 969
BtsIMutI CAGTG 7 cut(s) 216, 363, 969, 1269, 1830, 2077, 2228
BveI ACCTGC 3 cut(s) 804, 1098, 1421
Cac8I GCNNGC 3 cut(s) 801, 906, 1371
CaiI CAGNNNCTG 3 cut(s) 1044, 1436, 1514
CfoI GCGC 1 cut(s) 976
Cfr10I RCCGGY 1 cut(s) 2017
Cfr13I GGNCC 8 cut(s) 1007, 1058, 1175, 1658, 1748, 1763, 1764, 1859
CseI GACGC 2 cut(s) 240, 1320
Csp6I GTAC 5 cut(s) 292, 924, 982, 1140, 1801
CviQI GTAC 5 cut(s) 292, 924, 982, 1140, 1801
DrdI GACNNNNNNGTC 1 cut(s) 250
DriI GACNNNNNGTC 1 cut(s) 2248
DseDI GACNNNNNNGTC 1 cut(s) 250
EaeI YGGCCR 4 cut(s) 567, 821, 1371, 1995
Eam1104I CTCTTC 7 cut(s) 15, 254, 936, 1401, 1695, 1963, 2303
Eam1105I GACNNNNNGTC 1 cut(s) 2248
EarI CTCTTC 7 cut(s) 15, 254, 936, 1401, 1695, 1963, 2303
Eco147I AGGCCT 1 cut(s) 457
Eco24I GRGCYC 1 cut(s) 1767
Eco31I GGTCTC 1 cut(s) 647
Eco32I GATATC 2 cut(s) 1498, 2302
Eco47I GGWCC 6 cut(s) 1007, 1058, 1175, 1658, 1748, 1859
Eco57I CTGAAG 2 cut(s) 278, 321
Eco81I CCTNAGG 2 cut(s) 2046, 2203
EcoO109I RGGNCCY 4 cut(s) 1007, 1175, 1763, 1859
EcoRII CCWGG 3 cut(s) 316, 1189, 2119
EcoRV GATATC 2 cut(s) 1498, 2302
EcoT22I ATGCAT 1 cut(s) 1984
EcoT38I GRGCYC 1 cut(s) 1767
Esp3I CGTCTC 2 cut(s) 258, 1081
FalI AAGNNNNNCTT 4 cut(s) 996, 1028, 2136, 2168
FaqI GGGAC 6 cut(s) 634, 993, 1172, 1558, 1727, 2255
FauI CCCGC 2 cut(s) 145, 1099
FbaI TGATCA 1 cut(s) 297
Fnu4HI GCNGC 8 cut(s) 123, 126, 351, 1029, 1032, 1651, 2196, 2283
FokI GGATG 6 cut(s) 41, 663, 1255, 1283, 1634, 1953
FriOI GRGCYC 1 cut(s) 1767
Fsp4HI GCNGC 8 cut(s) 123, 126, 351, 1029, 1032, 1651, 2196, 2283
FspBI CTAG 3 cut(s) 276, 1628, 2004
FspI TGCGCA 1 cut(s) 975
GlaI GCGC 1 cut(s) 975
GluI GCNGC 8 cut(s) 123, 126, 351, 1029, 1032, 1651, 2196, 2283
GsaI CCCAGC 1 cut(s) 1836
GsuI CTGGAG 1 cut(s) 1458
HaeIII GGCC 9 cut(s) 457, 569, 823, 1373, 1637, 1650, 1765, 1997, 2119
HapII CCGG 3 cut(s) 270, 1555, 2018
HgaI GACGC 2 cut(s) 240, 1320
HhaI GCGC 1 cut(s) 976
Hin6I GCGC 1 cut(s) 974
HinP1I GCGC 1 cut(s) 974
HincII GTYRAC 4 cut(s) 328, 388, 472, 1780
HindII GTYRAC 4 cut(s) 328, 388, 472, 1780
HindIII AAGCTT 2 cut(s) 836, 1346
HpaII CCGG 3 cut(s) 270, 1555, 2018
HpyAV CCTTC 9 cut(s) 392, 802, 998, 1129, 1349, 1349, 1640, 1850, 2162
HpyCH4IV ACGT 3 cut(s) 196, 324, 1074
HpyF10VI GCNNNNNNNGC 8 cut(s) 188, 635, 901, 910, 926, 1970, 1979, 2125
HpySE526I ACGT 3 cut(s) 196, 324, 1074
HspAI GCGC 1 cut(s) 974
KpnI GGTACC 1 cut(s) 985
Ksp22I TGATCA 1 cut(s) 297
LguI GCTCTTC 3 cut(s) 15, 936, 1963
LmnI GCTCC 4 cut(s) 272, 792, 1920, 2051
Lsp1109I GCAGC 7 cut(s) 109, 112, 337, 1015, 1043, 2182, 2269
LweI GCATC 6 cut(s) 238, 1056, 1103, 1662, 1915, 1991
MaeI CTAG 3 cut(s) 276, 1628, 2004
MaeII ACGT 3 cut(s) 196, 324, 1074
MaeIII GTNAC 3 cut(s) 59, 343, 1524
MbiI CCGCTC 1 cut(s) 929
MflI RGATCY 6 cut(s) 580, 1204, 1468, 1510, 1677, 1720
MhlI GDGCHC 5 cut(s) 376, 429, 1422, 1767, 1917
MlsI TGGCCA 2 cut(s) 569, 1373
MluCI AATT 5 cut(s) 33, 529, 670, 1455, 2360
MluNI TGGCCA 2 cut(s) 569, 1373
MlyI GAGTC 5 cut(s) 305, 1418, 1814, 2174, 2329
Mox20I TGGCCA 2 cut(s) 569, 1373
Mph1103I ATGCAT 1 cut(s) 1984
MroXI GAANNNNTTC 1 cut(s) 263
MscI TGGCCA 2 cut(s) 569, 1373
MseI TTAA 2 cut(s) 501, 1362
MslI CAYNNNNRTG 2 cut(s) 1241, 1923
Msp20I TGGCCA 2 cut(s) 569, 1373
MspA1I CMGCKG 1 cut(s) 350
MspCI CTTAAG 2 cut(s) 500, 1361
MspI CCGG 3 cut(s) 270, 1555, 2018
MspR9I CCNGG 3 cut(s) 318, 1191, 2121
Mva1269I GAATGC 2 cut(s) 556, 1333
MvaI CCWGG 3 cut(s) 318, 1191, 2121
MwoI GCNNNNNNNGC 8 cut(s) 188, 635, 901, 910, 926, 1970, 1979, 2125
NlaIV GGNNCC 7 cut(s) 582, 788, 983, 1009, 1176, 1765, 1838
NmuCI GTSAC 1 cut(s) 343
NsbI TGCGCA 1 cut(s) 975
NsiI ATGCAT 1 cut(s) 1984
NspI RCATGY 3 cut(s) 67, 1786, 2099
PaqCI CACCTGC 1 cut(s) 1421
PceI AGGCCT 1 cut(s) 457
PciI ACATGT 3 cut(s) 63, 1782, 2095
PciSI GCTCTTC 3 cut(s) 15, 936, 1963
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PctI GAATGC 2 cut(s) 556, 1333
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 7 cut(s) 110, 710, 752, 1087, 1690, 2064, 2080
PflMI CCANNNNNTGG 2 cut(s) 768, 783
PfoI TCCNGGA 1 cut(s) 316
PkrI GCNGC 8 cut(s) 124, 127, 352, 1030, 1033, 1652, 2197, 2284
PleI GAGTC 5 cut(s) 305, 1417, 1814, 2173, 2329
PpsI GAGTC 5 cut(s) 305, 1417, 1814, 2173, 2329
PpuMI RGGWCCY 3 cut(s) 1007, 1175, 1859
PscI ACATGT 3 cut(s) 63, 1782, 2095
Psp5II RGGWCCY 3 cut(s) 1007, 1175, 1859
Psp6I CCWGG 3 cut(s) 316, 1189, 2119
PspFI CCCAGC 1 cut(s) 1832
PspGI CCWGG 3 cut(s) 316, 1189, 2119
PspN4I GGNNCC 7 cut(s) 582, 788, 983, 1009, 1176, 1765, 1838
PspOMI GGGCCC 1 cut(s) 1763
PspPI GGNCC 8 cut(s) 1007, 1058, 1175, 1658, 1748, 1763, 1764, 1859
PspPPI RGGWCCY 3 cut(s) 1007, 1175, 1859
PstI CTGCAG 1 cut(s) 1033
PstNI CAGNNNCTG 3 cut(s) 1044, 1436, 1514
PsuI RGATCY 6 cut(s) 580, 1204, 1468, 1510, 1677, 1720
PvuII CAGCTG 1 cut(s) 350
RsaI GTAC 5 cut(s) 293, 925, 983, 1141, 1802
RsaNI GTAC 5 cut(s) 292, 924, 982, 1140, 1801
RseI CAYNNNNRTG 2 cut(s) 1241, 1923
SapI GCTCTTC 3 cut(s) 15, 936, 1963
SaqAI TTAA 2 cut(s) 501, 1362
SatI GCNGC 8 cut(s) 123, 126, 351, 1029, 1032, 1651, 2196, 2283
Sau96I GGNCC 8 cut(s) 1007, 1058, 1175, 1658, 1748, 1763, 1764, 1859
ScaI AGTACT 1 cut(s) 1802
SchI GAGTC 5 cut(s) 305, 1418, 1814, 2174, 2329
ScrFI CCNGG 3 cut(s) 318, 1191, 2121
SduI GDGCHC 5 cut(s) 376, 429, 1422, 1767, 1917
SfaNI GCATC 6 cut(s) 238, 1056, 1103, 1662, 1915, 1991
SfcI CTRYAG 2 cut(s) 1029, 2068
SinI GGWCC 6 cut(s) 1007, 1058, 1175, 1658, 1748, 1859
SmiMI CAYNNNNRTG 2 cut(s) 1241, 1923
SmlI CTYRAG 6 cut(s) 141, 494, 500, 1361, 2012, 2264
SmoI CTYRAG 6 cut(s) 141, 494, 500, 1361, 2012, 2264
Sse9I AATT 5 cut(s) 33, 529, 670, 1455, 2360
SseBI AGGCCT 1 cut(s) 457
SsiI CCGC 6 cut(s) 152, 927, 1092, 1493, 1651, 2261
SspMI CTAG 3 cut(s) 276, 1628, 2004
StuI AGGCCT 1 cut(s) 457
StyD4I CCNGG 3 cut(s) 316, 1189, 2119
TaiI ACGT 3 cut(s) 199, 327, 1077
TaqI TCGA 3 cut(s) 84, 2031, 2163
TaqII GACCGA 2 cut(s) 1112, 1646
TasI AATT 5 cut(s) 33, 529, 670, 1455, 2360
TatI WGTACW 1 cut(s) 1800
TauI GCSGC 1 cut(s) 1653
TfiI GAWTC 7 cut(s) 110, 710, 752, 1087, 1690, 2064, 2080
Tru1I TTAA 2 cut(s) 501, 1362
Tru9I TTAA 2 cut(s) 501, 1362
TscAI CASTG 7 cut(s) 223, 370, 976, 1269, 1830, 2077, 2228
TseFI GTSAC 1 cut(s) 343
TseI GCWGC 7 cut(s) 122, 125, 350, 1028, 1031, 2195, 2282
Tsp45I GTSAC 1 cut(s) 343
TspDTI ATGAA 8 cut(s) 866, 905, 1017, 1172, 1306, 1659, 1824, 2367
TspGWI ACGGA 6 cut(s) 43, 47, 53, 122, 347, 663
TspRI CASTG 7 cut(s) 223, 370, 976, 1269, 1830, 2077, 2228
Van91I CCANNNNNTGG 2 cut(s) 768, 783
Vha464I CTTAAG 2 cut(s) 500, 1361
VpaK11BI GGWCC 6 cut(s) 1007, 1058, 1175, 1658, 1748, 1859
XapI RAATTY 2 cut(s) 529, 1455
XceI RCATGY 3 cut(s) 67, 1786, 2099
XcmI CCANNNNNNNNNTGG 2 cut(s) 86, 1940
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 3 cut(s) 276, 1628, 2004
ZrmI AGTACT 1 cut(s) 1802
Zsp2I ATGCAT 1 cut(s) 1984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.