MD05G1221000.v1.1

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001995
Physical Location & Seq
Reverse (-)
35355472 .. 35359007
3536 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1221000.v1.1.491

Sequence Viewer

Length: 2532 bp
ATGGTGAAGTTCACAGCTGAGGAGCTCCGTAGGATTATGGACTACAAACACAACATTCGTAATATGTCTGTTATTGCGCACGTCGACCATGGGAAGTCAACTCTTACCGACTCCCTTGTCGCTGCAGCTGGCATCATTGCACAGGAAGTTGCCGGTGATGTCCGTATGACGGATACCCGTGCAGATGAAGCTGAGCGTGGTATCACAATCAAATCTACCGGTATCTCTTTGTACTATGAGATGACTGATGAGTCTTTGTCGAGGTTCAAGGGAGAGAGAAACGGAAATGAGTACCTCATCAATCTCATTGATTCCCCCGGGCACGTTGACTTTTCATCTGAGGTCACAGCTGCTCTGCGTATTACTGATGGTGCACTTGTGGTGGTGGATTGTATTGAGGGTGTGTGTGTCCAGACTGAGACTGTGCTCCGTCAAGCCTTGGGAGAGAGGATCAGGCCTGTTTTGACCGTTAACAAGATGGACAGGTGCTTCCTTGAGCTCCAGGTCGATGGAGAAGAGGCTTACCAGACATTCCAAAGAGTTATTGAAAATGCCAATGTCATTATGGCTACATATGAAGATCCACTTCTTGGTGATGTCCAGGTGTACCCAGAGAAAGGAACCGTTGCCTTTTCAGCTGGTTTGCACGGTTGGGCTTTCACTCTGACCAACTTTGCTAAGATGTATGCTTCCAAGTTTGGAGTTGATGAGTCAAAGATGATGGAAAGGCTCTGGGGTGAGAACTTCTTTGACCCAGCTACCAAGAAATGGACCAGCAAGAACACTGGAACTGCTACTTGCAAGCGTGGTTTTGTTCAGTTCTGTTATGAGCCCATCAAGCAGATCATCAAAACCTGCATGAATGATCAGAAGGATAAGTTGTGGCCCATGTTGACAAAGCTTGGTGTCACCATGAAGAGTGATGAGAAGGACCTGATGGGGAAGGCGTTGATGAAGCGTGTTATGCAGACCTGGCTACCAGCTAGCACTGCTCTATTGGAAATGATGATCTTTCACCTTCCATCTCCCTCCACGGCTCAAAGGTACCGTGTTGAGAATTTGTACGAGGGTCCCCTTGATGATCAATATGCCAATGCCATCAGGAATTGTGACCCCGAAGGACCTCTTATGCTTTATGTGTCTAAGATGATTCCTGCTTCTGATAAGGGTAGATTCTTTGCCTTTGGCCGTGTCTTTGCTGGTAAAGTTCAGACAGGTTTGAAGGTTAGAATCATGGGTCCAAATTTTGTTCCTGGAGAAAAGAAGGATTTGTATGTCAAGAACGTACAGAGGACGGTTATTTGGATGGGAAAGAGACAAGAAACTGTTGAGGATGTTCCTTGTGGTAACACTGTTGCTTTGGTCGGTTTGGATCAGTTTATCACCAAGAATGCTACTTTAACAAATGAGAAGGAAGTAGATGCTCACCCTATTCGTGCTATGAAGTTCTCTGTTTCACCTGTCGTGCGTGTTGCTGTCCAGTGCAAGGTCGCATCTGACCTTCCCAAACTTGTTGAAGGTCTCAAACGTTTGGCCAAGTCTGATCCTATGGTTGTCTGCTCCATTGAGGAGTCTGGAGAGCACATTATTGCTGGTGCTGGTGAACTGCATCTTGAGATATGTTTGAAGGATCTGCAAGATGATTTTATGGGTGGTGCCGAGATTATAAAGTCTGACCCTGTTGTGTCCTTCCGTGAGACTGTCCTTGATAAGTCCTGCCGTACAGTGATGAGCAAGTCCCCAAACAAGCATAATCGTCTGTACATGGAAGCAAGGCCCCTTGAGGAAGGTCTTCCCGAGGCTATTGATGAAGGTCGCATTGGCCCAAGAGATGATCCCAAGATTCGTTCCAAGATCTTGGCTGAGGAGTTTGGTTGGGACAAGGATCTTGCTAAGAAAATCTGGTGTTTTGGTCCCGAGACGACTGGTCCTAACATGGTGGTCGACATGTGTAAGGGAGTTCAGTACCTTAATGAAATCAAGGATTCTGTTGTTGCTGGGTTCCAGTGGGCCTCAAAGGAAGGTGCATTGGCTGAAGAAAACATGAGAGGTATTTGCTTTGAAGTCTGTGATGTGGTTCTTCATGCTGATGCTATCCACAGAGGGGGTGGTCAGGTCATTCCAACCGCTCGAAGGGTCATCTATGCCTCTCAGCTTACAGCCAAGCCAAGGCTTCTCGAACCAGTTTATCTCGTTGAAATTCAAGCTCCAGAGGGGGCTCTTGGCGGTATCTACAGTGTTCTGAATCAGAAACGTGGGCACGTGTTTGAGGAGATGCAAAGGCCTGGAACTCCACTCTACAACATCAAGGCATACCTCCCTGTCGTTGAGTCCTTTGGATTCTCTGGTCAACTGAGGGCTGCAACTTCTGGGCAGGCTTTCCCGCAATGTGTGTTTGATCATTGGGAGATGATGTCGTCCGATCCATTGGAAGCGGGATCCCAGGCTGCAGCACTTGTTACCGACATCCGTAAGAGGAAGGGATTGAAGGAGCAGATGACCCCGCTATCCGAGTTCGAGGACAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04152 ko04921 map04152 map04921
Pfam Domains
Protein Families
EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

844

Amino Acids

94.01

Weight (kDa)

5.86

Isoelectric Point (pI)

33.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 18 - 227 1.4e-48 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 379 - 469 4.1e-06 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 394 - 462 5e-08 Elongation factor Tu domain 2
EFG_III PF14492 487 - 549 1e-11 Elongation Factor G, domain III
EFG_IV PF03764 610 - 722 2e-29 Elongation factor G, domain IV
EFG_C PF00679 724 - 811 1.9e-20 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1667
AasI GACNNNNNNGTC 2 cut(s) 116, 250
Acc16I TGCGCA 1 cut(s) 78
Acc36I ACCTGC 1 cut(s) 863
Acc65I GGTACC 1 cut(s) 1044
AccB1I GGYRCC 2 cut(s) 1044, 1655
AccB7I CCANNNNNTGG 2 cut(s) 590, 768
AccBSI CCGCTC 1 cut(s) 2129
AccI GTMKAC 2 cut(s) 84, 1944
AciI CCGC 5 cut(s) 2127, 2226, 2384, 2435, 2504
AclI AACGTT 1 cut(s) 1528
AcoI YGGCCR 2 cut(s) 1186, 1533
AcsI RAATTY 3 cut(s) 1057, 1243, 2199
AcuI CTGAAG 1 cut(s) 2055
AcvI CACGTG 1 cut(s) 2263
AfaI GTAC 9 cut(s) 233, 293, 608, 1046, 1064, 1287, 1723, 1763, 1967
AfiI CCNNNNNNNGG 8 cut(s) 169, 590, 617, 768, 1486, 2104, 2133, 2169
AflIII ACRYGT 2 cut(s) 1947, 2262
AgeI ACCGGT 1 cut(s) 218
AgsI TTSAA 9 cut(s) 268, 548, 1222, 1517, 1627, 2063, 2198, 2204, 2488
AhdI GACNNNNNGTC 1 cut(s) 1926
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 6 cut(s) 501, 600, 971, 1252, 2284, 2442
AjuI GAANNNNNNNTTGG 2 cut(s) 1803, 1835
Alw21I GWGCWC 5 cut(s) 27, 376, 429, 501, 1584
Alw26I GTCTC 5 cut(s) 413, 1309, 1526, 1691, 1913
Alw44I GTGCAC 1 cut(s) 372
Ama87I CYCGRG 3 cut(s) 317, 1796, 1916
AoxI GGCC 8 cut(s) 455, 884, 1186, 1533, 1775, 1822, 2010, 2282
ApaLI GTGCAC 1 cut(s) 372
ApeKI GCWGC 6 cut(s) 122, 125, 350, 2360, 2447, 2450
ApoI RAATTY 3 cut(s) 1057, 1243, 2199
AsiGI ACCGGT 1 cut(s) 218
Asp700I GAANNNNTTC 1 cut(s) 1791
Asp718I GGTACC 1 cut(s) 1044
AspLEI GCGC 1 cut(s) 79
AsuC2I CCSGG 2 cut(s) 318, 319
AsuNHI GCTAGC 1 cut(s) 983
AvaI CYCGRG 3 cut(s) 317, 1796, 1916
AvaII GGWCC 7 cut(s) 771, 931, 1070, 1121, 1238, 1913, 1928
BaeGI GKGCMC 3 cut(s) 324, 376, 2262
BalI TGGCCA 1 cut(s) 1535
BamHI GGATCC 1 cut(s) 2438
BanI GGYRCC 2 cut(s) 1044, 1655
BanII GRGCYC 4 cut(s) 27, 501, 834, 2221
BbrPI CACGTG 1 cut(s) 2263
BbsI GAAGAC 1 cut(s) 1784
Bbv12I GWGCWC 5 cut(s) 27, 376, 429, 501, 1584
BbvCI CCTCAGC 2 cut(s) 18, 1863
BbvI GCAGC 6 cut(s) 109, 137, 337, 2347, 2434, 2462
BccI CCATC 9 cut(s) 362, 472, 503, 715, 842, 931, 1030, 1106, 1300
BceAI ACGGC 3 cut(s) 1050, 1173, 1704
BciT130I CCWGG 6 cut(s) 503, 602, 973, 1254, 2286, 2444
BciVI GTATCC 1 cut(s) 166
BclI TGATCA 3 cut(s) 865, 1081, 2398
BcnI CCSGG 2 cut(s) 318, 319
BcoDI GTCTC 5 cut(s) 413, 1309, 1526, 1691, 1913
BfaI CTAG 1 cut(s) 984
BfmI CTRYAG 3 cut(s) 123, 2233, 2448
BfuAI ACCTGC 1 cut(s) 863
BfuI GTATCC 1 cut(s) 166
BglII AGATCT 1 cut(s) 1854
BisI GCNGC 6 cut(s) 123, 126, 351, 2361, 2448, 2451
BlpI GCTNAGC 1 cut(s) 192
BlsI GCNGC 6 cut(s) 124, 127, 352, 2362, 2449, 2452
Bme1390I CCNGG 8 cut(s) 318, 319, 503, 602, 973, 1254, 2286, 2444
Bme18I GGWCC 7 cut(s) 771, 931, 1070, 1121, 1238, 1913, 1928
BmeRI GACNNNNNGTC 1 cut(s) 1926
BmeT110I CYCGRG 3 cut(s) 317, 1796, 1916
BmgBI CACGTC 1 cut(s) 82
BmrFI CCNGG 8 cut(s) 318, 319, 503, 602, 973, 1254, 2286, 2444
BmsI GCATC 6 cut(s) 141, 1411, 1502, 1618, 2080, 2265
BmtI GCTAGC 1 cut(s) 987
BpiI GAAGAC 1 cut(s) 1784
BpmI CTGGAG 4 cut(s) 485, 1275, 1596, 2193
Bpu10I CCTNAGC 2 cut(s) 18, 1863
Bpu1102I GCTNAGC 1 cut(s) 192
BpuEI CTTGAG 3 cut(s) 515, 1634, 1802
BpuMI CCSGG 2 cut(s) 318, 319
BsaAI YACGTR 1 cut(s) 2263
BsaI GGTCTC 1 cut(s) 1526
BsaJI CCNNGG 8 cut(s) 88, 316, 317, 438, 1032, 1797, 2168, 2442
BsaWI WCCGGW 1 cut(s) 218
BsaXI ACNNNNNCTCC 2 cut(s) 2399, 2429
Bsc4I CCNNNNNNNGG 8 cut(s) 169, 590, 617, 768, 1486, 2104, 2133, 2169
Bse118I RCCGGY 2 cut(s) 152, 218
Bse1I ACTGG 5 cut(s) 790, 1480, 1930, 2005, 2183
Bse3DI GCAATG 2 cut(s) 135, 2393
BseBI CCWGG 6 cut(s) 503, 602, 973, 1254, 2286, 2444
BseDI CCNNGG 8 cut(s) 88, 316, 317, 438, 1032, 1797, 2168, 2442
BseGI GGATG 3 cut(s) 1311, 1339, 2466
BseLI CCNNNNNNNGG 8 cut(s) 169, 590, 617, 768, 1486, 2104, 2133, 2169
BseMI GCAATG 2 cut(s) 135, 2393
BseMII CTCAG 7 cut(s) 9, 183, 330, 408, 1854, 2165, 2345
BseNI ACTGG 5 cut(s) 790, 1480, 1930, 2005, 2183
BseRI GAGGAG 4 cut(s) 35, 1583, 1880, 2285
BseSI GKGCMC 3 cut(s) 324, 376, 2262
BseXI GCAGC 6 cut(s) 109, 137, 337, 2347, 2434, 2462
BseYI CCCAGC 2 cut(s) 754, 1997
BsgI GTGCAG 1 cut(s) 201
BshFI GGCC 8 cut(s) 457, 886, 1188, 1535, 1777, 1824, 2012, 2284
BshNI GGYRCC 2 cut(s) 1044, 1655
BshTI ACCGGT 1 cut(s) 218
BsiHKAI GWGCWC 5 cut(s) 27, 376, 429, 501, 1584
BsiHKCI CYCGRG 3 cut(s) 317, 1796, 1916
BsiSI CCGG 3 cut(s) 153, 219, 318
BslFI GGGAC 4 cut(s) 1056, 1723, 1892, 1899
BslI CCNNNNNNNGG 8 cut(s) 169, 590, 617, 768, 1486, 2104, 2133, 2169
BsmAI GTCTC 5 cut(s) 413, 1309, 1526, 1691, 1913
BsmBI CGTCTC 1 cut(s) 1913
BsmFI GGGAC 4 cut(s) 1056, 1723, 1892, 1899
BsmI GAATGC 1 cut(s) 1396
BsnI GGCC 8 cut(s) 457, 886, 1188, 1535, 1777, 1824, 2012, 2284
Bso31I GGTCTC 1 cut(s) 1526
BsoBI CYCGRG 3 cut(s) 317, 1796, 1916
Bsp1286I GDGCHC 9 cut(s) 27, 324, 376, 429, 501, 834, 1584, 2221, 2262
Bsp1407I TGTACA 1 cut(s) 1761
Bsp1720I GCTNAGC 1 cut(s) 192
Bsp19I CCATGG 1 cut(s) 88
BspACI CCGC 5 cut(s) 2127, 2226, 2384, 2435, 2504
BspANI GGCC 8 cut(s) 457, 886, 1188, 1535, 1777, 1824, 2012, 2284
BspCNI CTCAG 7 cut(s) 10, 184, 331, 409, 1855, 2164, 2346
BspMAI CTGCAG 2 cut(s) 127, 2452
BspMI ACCTGC 1 cut(s) 863
BspOI GCTAGC 1 cut(s) 987
BspT107I GGYRCC 2 cut(s) 1044, 1655
BspTNI GGTCTC 1 cut(s) 1526
BsrBI CCGCTC 1 cut(s) 2129
BsrDI GCAATG 2 cut(s) 135, 2393
BsrFI RCCGGY 2 cut(s) 152, 218
BsrGI TGTACA 1 cut(s) 1761
BsrI ACTGG 5 cut(s) 790, 1480, 1930, 2005, 2183
BssAI RCCGGY 2 cut(s) 152, 218
BssECI CCNNGG 8 cut(s) 88, 316, 317, 438, 1032, 1797, 2168, 2442
BssT1I CCWWGG 3 cut(s) 88, 438, 2168
Bst2UI CCWGG 6 cut(s) 503, 602, 973, 1254, 2286, 2444
Bst6I CTCTTC 2 cut(s) 510, 911
BstAUI TGTACA 1 cut(s) 1761
BstBAI YACGTR 1 cut(s) 2263
BstC8I GCNNGC 4 cut(s) 130, 803, 985, 2376
BstDSI CCRYGG 2 cut(s) 88, 1032
BstF5I GGATG 3 cut(s) 1311, 1339, 2466
BstHHI GCGC 1 cut(s) 79
BstMAI GTCTC 5 cut(s) 413, 1309, 1526, 1691, 1913
BstMWI GCNNNNNNNGC 6 cut(s) 188, 635, 838, 964, 973, 989
BstNI CCWGG 6 cut(s) 503, 602, 973, 1254, 2286, 2444
BstNSI RCATGY 1 cut(s) 1951
BstSCI CCNGG 8 cut(s) 316, 317, 501, 600, 971, 1252, 2284, 2442
BstSFI CTRYAG 3 cut(s) 123, 2233, 2448
BstSLI GKGCMC 3 cut(s) 324, 376, 2262
BstV1I GCAGC 6 cut(s) 109, 137, 337, 2347, 2434, 2462
BstV2I GAAGAC 1 cut(s) 1784
BstX2I RGATCY 5 cut(s) 580, 1630, 1854, 1885, 2438
BstXI CCANNNNNNTGG 2 cut(s) 509, 1858
BstYI RGATCY 5 cut(s) 580, 1630, 1854, 1885, 2438
BsuI GTATCC 1 cut(s) 166
BsuRI GGCC 8 cut(s) 457, 886, 1188, 1535, 1777, 1824, 2012, 2284
BtgI CCRYGG 2 cut(s) 88, 1032
BtrI CACGTC 1 cut(s) 82
BtsCI GGATG 3 cut(s) 1311, 1339, 2466
BtsI GCAGTG 1 cut(s) 987
BtsIMutI CAGTG 7 cut(s) 783, 987, 1350, 1487, 1731, 2012, 2242
BveI ACCTGC 1 cut(s) 863
Cac8I GCNNGC 4 cut(s) 130, 803, 985, 2376
CfoI GCGC 1 cut(s) 79
Cfr10I RCCGGY 2 cut(s) 152, 218
Cfr9I CCCGGG 1 cut(s) 317
Csp6I GTAC 9 cut(s) 232, 292, 607, 1045, 1063, 1286, 1722, 1762, 1966
CspAI ACCGGT 1 cut(s) 218
CviQI GTAC 9 cut(s) 232, 292, 607, 1045, 1063, 1286, 1722, 1762, 1966
DrdI GACNNNNNNGTC 2 cut(s) 116, 250
DriI GACNNNNNGTC 1 cut(s) 1926
DseDI GACNNNNNNGTC 2 cut(s) 116, 250
EaeI YGGCCR 2 cut(s) 1186, 1533
Eam1104I CTCTTC 2 cut(s) 510, 911
Eam1105I GACNNNNNGTC 1 cut(s) 1926
EarI CTCTTC 2 cut(s) 510, 911
Ecl136II GAGCTC 2 cut(s) 25, 499
Eco130I CCWWGG 3 cut(s) 88, 438, 2168
Eco147I AGGCCT 2 cut(s) 457, 2284
Eco24I GRGCYC 4 cut(s) 27, 501, 834, 2221
Eco31I GGTCTC 1 cut(s) 1526
Eco47I GGWCC 7 cut(s) 771, 931, 1070, 1121, 1238, 1913, 1928
Eco53kI GAGCTC 2 cut(s) 25, 499
Eco57I CTGAAG 1 cut(s) 2055
Eco72I CACGTG 1 cut(s) 2263
Eco88I CYCGRG 3 cut(s) 317, 1796, 1916
EcoICRI GAGCTC 2 cut(s) 25, 499
EcoO109I RGGNCCY 4 cut(s) 931, 1070, 1121, 1776
EcoRII CCWGG 6 cut(s) 501, 600, 971, 1252, 2284, 2442
EcoT14I CCWWGG 3 cut(s) 88, 438, 2168
EcoT38I GRGCYC 4 cut(s) 27, 501, 834, 2221
ErhI CCWWGG 3 cut(s) 88, 438, 2168
Esp3I CGTCTC 1 cut(s) 1913
FalI AAGNNNNNCTT 4 cut(s) 570, 602, 1110, 1142
FaqI GGGAC 4 cut(s) 1056, 1723, 1892, 1899
FauI CCCGC 3 cut(s) 2391, 2428, 2511
FauNDI CATATG 1 cut(s) 574
FbaI TGATCA 3 cut(s) 865, 1081, 2398
FblI GTMKAC 2 cut(s) 84, 1944
Fnu4HI GCNGC 6 cut(s) 123, 126, 351, 2361, 2448, 2451
FokI GGATG 3 cut(s) 1318, 1346, 2453
FriOI GRGCYC 4 cut(s) 27, 501, 834, 2221
Fsp4HI GCNGC 6 cut(s) 123, 126, 351, 2361, 2448, 2451
FspBI CTAG 1 cut(s) 984
FspI TGCGCA 1 cut(s) 78
GlaI GCGC 1 cut(s) 78
GluI GCNGC 6 cut(s) 123, 126, 351, 2361, 2448, 2451
GsaI CCCAGC 2 cut(s) 758, 2001
GsuI CTGGAG 4 cut(s) 485, 1275, 1596, 2193
HaeIII GGCC 8 cut(s) 457, 886, 1188, 1535, 1777, 1824, 2012, 2284
HapII CCGG 3 cut(s) 153, 219, 318
HhaI GCGC 1 cut(s) 79
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HincII GTYRAC 7 cut(s) 85, 99, 328, 472, 894, 1945, 2351
HindII GTYRAC 7 cut(s) 85, 99, 328, 472, 894, 1945, 2351
HindIII AAGCTT 2 cut(s) 899, 2525
HpaI GTTAAC 1 cut(s) 472
HpaII CCGG 3 cut(s) 153, 219, 318
Hpy188III TCNNGA 9 cut(s) 412, 1102, 1279, 1575, 1613, 1796, 1916, 2177, 2210
Hpy99I CGWCG 1 cut(s) 86
HpyCH4IV ACGT 6 cut(s) 81, 324, 1284, 1528, 2254, 2262
HpyF10VI GCNNNNNNNGC 6 cut(s) 188, 635, 838, 964, 973, 989
HpySE526I ACGT 6 cut(s) 81, 324, 1284, 1528, 2254, 2262
HspAI GCGC 1 cut(s) 77
KflI GGGWCCC 1 cut(s) 1070
KpnI GGTACC 1 cut(s) 1048
Ksp22I TGATCA 3 cut(s) 865, 1081, 2398
KspAI GTTAAC 1 cut(s) 472
LmnI GCTCC 7 cut(s) 22, 30, 432, 504, 1565, 2212, 2491
Lsp1109I GCAGC 6 cut(s) 109, 137, 337, 2347, 2434, 2462
LweI GCATC 6 cut(s) 141, 1411, 1502, 1618, 2080, 2265
MaeI CTAG 1 cut(s) 984
MaeII ACGT 6 cut(s) 81, 324, 1284, 1528, 2254, 2262
MaeIII GTNAC 5 cut(s) 343, 907, 1109, 1346, 2458
MbiI CCGCTC 1 cut(s) 2129
MboII GAAGA 6 cut(s) 527, 590, 928, 1784, 2048, 2072
MflI RGATCY 5 cut(s) 580, 1630, 1854, 1885, 2438
MhlI GDGCHC 9 cut(s) 27, 324, 376, 429, 501, 834, 1584, 2221, 2262
MlsI TGGCCA 1 cut(s) 1535
MluCI AATT 4 cut(s) 1057, 1105, 1243, 2199
MluNI TGGCCA 1 cut(s) 1535
MlyI GAGTC 5 cut(s) 104, 260, 719, 1580, 2339
MmeI TCCRAC 1 cut(s) 2147
Mox20I TGGCCA 1 cut(s) 1535
MroXI GAANNNNTTC 1 cut(s) 1791
MscI TGGCCA 1 cut(s) 1535
MseI TTAA 3 cut(s) 471, 1400, 1971
MslI CAYNNNNRTG 1 cut(s) 2088
Msp20I TGGCCA 1 cut(s) 1535
MspA1I CMGCKG 4 cut(s) 17, 128, 350, 638
MspI CCGG 3 cut(s) 153, 219, 318
MspR9I CCNGG 8 cut(s) 318, 319, 503, 602, 973, 1254, 2286, 2444
Mva1269I GAATGC 1 cut(s) 1396
MvaI CCWGG 6 cut(s) 503, 602, 973, 1254, 2286, 2444
MwoI GCNNNNNNNGC 6 cut(s) 188, 635, 838, 964, 973, 989
NciI CCSGG 2 cut(s) 318, 319
NcoI CCATGG 1 cut(s) 88
NdeI CATATG 1 cut(s) 574
NheI GCTAGC 1 cut(s) 983
NmeAIII GCCGAG 1 cut(s) 1684
NmuCI GTSAC 3 cut(s) 343, 907, 1109
NsbI TGCGCA 1 cut(s) 78
NspI RCATGY 1 cut(s) 1951
PceI AGGCCT 2 cut(s) 457, 2284
PciI ACATGT 1 cut(s) 1947
PctI GAATGC 1 cut(s) 1396
PdmI GAANNNNTTC 1 cut(s) 1791
PfeI GAWTC 8 cut(s) 311, 1150, 1173, 1230, 1843, 1985, 2245, 2340
PflMI CCANNNNNTGG 2 cut(s) 590, 768
PfoI TCCNGGA 1 cut(s) 1252
PinAI ACCGGT 1 cut(s) 218
PkrI GCNGC 6 cut(s) 124, 127, 352, 2362, 2449, 2452
PleI GAGTC 5 cut(s) 104, 259, 718, 1579, 2338
PmaCI CACGTG 1 cut(s) 2263
PmlI CACGTG 1 cut(s) 2263
PpsI GAGTC 5 cut(s) 104, 259, 718, 1579, 2338
Ppu21I YACGTR 1 cut(s) 2263
PpuMI RGGWCCY 3 cut(s) 931, 1070, 1121
PscI ACATGT 1 cut(s) 1947
PsiI TTATAA 1 cut(s) 1667
Psp124BI GAGCTC 2 cut(s) 27, 501
Psp1406I AACGTT 1 cut(s) 1528
Psp5II RGGWCCY 3 cut(s) 931, 1070, 1121
Psp6I CCWGG 6 cut(s) 501, 600, 971, 1252, 2284, 2442
PspCI CACGTG 1 cut(s) 2263
PspFI CCCAGC 2 cut(s) 754, 1997
PspGI CCWGG 6 cut(s) 501, 600, 971, 1252, 2284, 2442
PspPPI RGGWCCY 3 cut(s) 931, 1070, 1121
PstI CTGCAG 2 cut(s) 127, 2452
PsuI RGATCY 5 cut(s) 580, 1630, 1854, 1885, 2438
PvuII CAGCTG 4 cut(s) 17, 128, 350, 638
RsaI GTAC 9 cut(s) 233, 293, 608, 1046, 1064, 1287, 1723, 1763, 1967
RsaNI GTAC 9 cut(s) 232, 292, 607, 1045, 1063, 1286, 1722, 1762, 1966
RseI CAYNNNNRTG 1 cut(s) 2088
SacI GAGCTC 2 cut(s) 27, 501
SalI GTCGAC 2 cut(s) 83, 1943
SaqAI TTAA 3 cut(s) 471, 1400, 1971
SatI GCNGC 6 cut(s) 123, 126, 351, 2361, 2448, 2451
SchI GAGTC 5 cut(s) 104, 260, 719, 1580, 2339
ScrFI CCNGG 8 cut(s) 318, 319, 503, 602, 973, 1254, 2286, 2444
SduI GDGCHC 9 cut(s) 27, 324, 376, 429, 501, 834, 1584, 2221, 2262
SfaNI GCATC 6 cut(s) 141, 1411, 1502, 1618, 2080, 2265
SfcI CTRYAG 3 cut(s) 123, 2233, 2448
SinI GGWCC 7 cut(s) 771, 931, 1070, 1121, 1238, 1913, 1928
SmaI CCCGGG 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 2088
SmlI CTYRAG 3 cut(s) 494, 1613, 1781
SmoI CTYRAG 3 cut(s) 494, 1613, 1781
Sse9I AATT 4 cut(s) 1057, 1105, 1243, 2199
SseBI AGGCCT 2 cut(s) 457, 2284
SsiI CCGC 5 cut(s) 2127, 2226, 2384, 2435, 2504
SspMI CTAG 1 cut(s) 984
SstI GAGCTC 2 cut(s) 27, 501
StuI AGGCCT 2 cut(s) 457, 2284
StyD4I CCNGG 8 cut(s) 316, 317, 501, 600, 971, 1252, 2284, 2442
StyI CCWWGG 3 cut(s) 88, 438, 2168
TaiI ACGT 6 cut(s) 84, 327, 1287, 1531, 2257, 2265
TaqI TCGA 7 cut(s) 84, 260, 507, 1944, 2131, 2178, 2517
TasI AATT 4 cut(s) 1057, 1105, 1243, 2199
TatI WGTACW 2 cut(s) 231, 1761
TfiI GAWTC 8 cut(s) 311, 1150, 1173, 1230, 1843, 1985, 2245, 2340
Tru1I TTAA 3 cut(s) 471, 1400, 1971
Tru9I TTAA 3 cut(s) 471, 1400, 1971
TscAI CASTG 7 cut(s) 790, 994, 1357, 1487, 1731, 2012, 2242
TseFI GTSAC 3 cut(s) 343, 907, 1109
TseI GCWGC 6 cut(s) 122, 125, 350, 2360, 2447, 2450
Tsp45I GTSAC 3 cut(s) 343, 907, 1109
TspGWI ACGGA 7 cut(s) 17, 152, 185, 297, 419, 1682, 2459
TspMI CCCGGG 1 cut(s) 317
TspRI CASTG 7 cut(s) 790, 994, 1357, 1487, 1731, 2012, 2242
Van91I CCANNNNNTGG 2 cut(s) 590, 768
VneI GTGCAC 1 cut(s) 372
VpaK11BI GGWCC 7 cut(s) 771, 931, 1070, 1121, 1238, 1913, 1928
XapI RAATTY 3 cut(s) 1057, 1243, 2199
XceI RCATGY 1 cut(s) 1951
XcmI CCANNNNNNNNNTGG 2 cut(s) 562, 2105
XmaI CCCGGG 1 cut(s) 317
XmiI GTMKAC 2 cut(s) 84, 1944
XmnI GAANNNNTTC 1 cut(s) 1791
XspI CTAG 1 cut(s) 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.