MD10G1203200.v1.1

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001995
Physical Location & Seq
Reverse (-)
30091048 .. 30095250
4203 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1203200.v1.1.491

Sequence Viewer

Length: 2532 bp
ATGGTGAAGTTTACAGCAGAAGAACTCCGTAGGATTATGGACTACAAGCACAACATTCGCAATATGTCTGTTATTGCTCATGTTGATCACGGGAAGTCAACTCTTACAGACTCGCTTGTTGCTGCTGCGGGTATCATTGCACAAGAAGTTGCTGGTGATGTTCGGATGACTGATACTCGCGCAGATGAGGCAGAGCGTGGTATCACAATTAAATCTACCGGTATCTCTCTGTACTATGAGATGACTGATGAGTCTTTGCAGAGGTACAAGGGAGAGAGGAACGGAAATGAGTACCTCATCAATCTCATTGATTCCCCCGGGCACGTTGACTTTTCATCTGAAGTCACAGCTGCTCTGCGTATTACTGATGGAGCACTTGTGGTGGTGGATTGTATTGAGGGTGTGTGTGTCCAGACTGAGACTGTGCTCCGTCAAGCATTGGGAGAGAGGATCAGGCCTGTTTTGACCGTTAACAAGATGGACAGATGCTTCCTTGAGCTCCAGGTTGATGGAGAGGAAGCTTACCAGACATTCCAAAGGGTTATTGAGAATGCTAATGTCATTATGGCTACATATGAAGATCCACTTCTTGGTGATGTCCAAGTGTACCCAGAGAAAGGAACAGTCGCCTTCTCAGCTGGTTTGCACGGTTGGGCTTTCACTCTGACCAACTTCGCTAAGATGTATGCTTCCAAGTTTGGAGTTGATGAGTCAAAGATGATGGAAAGGCTCTGGGGTGAGAACTTTTTTGACCCAGCTACGAAGAAATGGACCAGCAAGAGCACCGGCACTGCTACCTGCAAGCGTGGTTTTGTTCAGTTCTGTTACGAACCCATCAAGCAGATCATCAACACCTGCATGAATGATCAGAAGGATAAGTTGTGGCCCATGTTGACAAAGCTTGGTGTCACCATGAAGAGTGATGAGAAGGACCTGATGGGGAAGGCTCTGATGAAGCGTGTTATGCAGACCTGGTTACCAGCTAGCACTGCTTTATTGGAAATGATGATTTTTCACCTTCCATCTCCGTCAACAGCTCAAAAGTACCGTGTTGAGAATTTGTACGAGGGTCCTCTTGATGATCAATATGCCAATGCCATCAGGAACTGTGACCCCGATGGACCTCTTATGCTCTATGTGTCCAAGATGATTCCCGCTTCTGATAAGGGTAGATTCTTTGCCTTTGGCCGTGTCTTTGCTGGTAGAGTTCAGACAGGATTGAAGGTTAGAATCATGGGTCCAAATTTTGTTCCCGGAGAGAAGAAGGATTTGTACGTTAAGAACGTACAGAGGACTGTTATTTGGATGGGAAAGAGACAAGAAACTGTTGAGGATGTTCCTTGCGGTAACACTGTGGCATTGGTCGGTCTGGATCAGTTTATTACCAAGAATGCAACTTTAACTAATGAGAAGGAAGTGGATGCTCACCCTATCCGTGCTATGAAGTTCTCCGTTTCACCTGTCGTGCGTGTTGCTGTCCAGTGCAAGGTTGCATCTGACCTTCCCAAACTTGTTGAAGGTCTCAAACGTTTGGCCAAGTCTGATCCTATGGTTGTCTGCTCCATTGAGGAGTCTGGAGAGCACATTATTGCTGGTGCTGGTGAACTGCATCTTGAGATCTGTTTGAAGGATCTACAAGATGATTTTATGGGTGGTGCTGAGATTATAAAGTCTGACCCCGTTGTGTCCTTCCGTGAGACTGTCCTTGATAAGTCCTGCCGTACAGTGATGAGTAAGTCTCCAAACAAGCATAACCGTCTGTACATGGAAGCAAGGCCCCTTGAGGAAGGTCTTCCTGAGGCTATTGATGAAGGTCGCATTGGCCCAAGAGATGATCCCAAGATTCGTTCCAAGATCTTGGCTGAGGAGTTTGGTTGGGACAAGGATCTTGCTAAGAAAATCTGGTGTTTTGGTCCCGAGACCACCGGTCCCAACATGGTGGTCGACATGTGTAAGGGAGTTCAGTACCTTAATGAAATCAAGGATTCGGTTGTTGCTGGGTTCCAGTGGGCCTCAAAGGAAGGTGCATTGGCAGAAGAAAACATGAGAGGTATTTGCTTTGAAGTCTGTGATGTGGTTCTTCACGCTGATGCTATCCACAGAGGGGGAGGTCAGGTCATTCCAACTGCTCGGAGGGTCATCTATGCCTCTCAGCTCACAGCCAAGCCAAGGCTTCTCGAACCAGTGTATCTCGTTGAAATTCAAGCTCCAGAGGGGGCACTTGGCGGTATCTACAGTGTTCTGAATCAGAAACGTGGGCATGTGTTTGAGGAGATGCAGAGGCCAGGAACTCCACTTTACAACATCAAGGCATACCTCCCCGTCGTTGAGTCCTTTGGATTCTCTGGTCAACTGAGGGCTGCAACTTCCGGGCAGGCCTTCCCACAATGCGTGTTTGATCATTGGGAGATGATGTCGTCTGATCCATTGGAAGCTGGATCCCAGGCTGCAACACTTGTCACCGACATCCGTAAGAGGAAGGGTTTGAAGGAGCAGATGACCCCGCTCTCTGAGTTCGAGGACAAGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04152 ko04921 map04152 map04921
Pfam Domains
Protein Families
EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

844

Amino Acids

94.04

Weight (kDa)

5.8

Isoelectric Point (pI)

33.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 18 - 227 1.4e-48 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 379 - 469 5.5e-06 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 394 - 462 1.1e-07 Elongation factor Tu domain 2
EFG_III PF14492 487 - 549 1e-11 Elongation Factor G, domain III
EFG_IV PF03764 610 - 722 2e-29 Elongation factor G, domain IV
EFG_C PF00679 724 - 811 1.9e-20 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1667
AarI CACCTGC 1 cut(s) 863
AasI GACNNNNNNGTC 1 cut(s) 250
Acc36I ACCTGC 2 cut(s) 806, 863
AccB7I CCANNNNNTGG 1 cut(s) 590
AccBSI CCGCTC 1 cut(s) 2506
AccI GTMKAC 1 cut(s) 1944
AccII CGCG 1 cut(s) 180
AciI CCGC 5 cut(s) 128, 1155, 1344, 2226, 2504
AclI AACGTT 1 cut(s) 1528
AcoI YGGCCR 2 cut(s) 1186, 1533
AcsI RAATTY 3 cut(s) 1057, 1243, 2199
AcuI CTGAAG 1 cut(s) 360
AfiI CCNNNNNNNGG 5 cut(s) 590, 617, 1486, 2104, 2169
AflIII ACRYGT 1 cut(s) 1947
AgeI ACCGGT 2 cut(s) 218, 1925
AgsI TTSAA 7 cut(s) 1222, 1517, 1627, 2063, 2198, 2204, 2488
AhdI GACNNNNNGTC 1 cut(s) 1926
AjnI CCWGG 4 cut(s) 501, 971, 2284, 2442
AjuI GAANNNNNNNTTGG 2 cut(s) 1803, 1835
Alw21I GWGCWC 5 cut(s) 376, 429, 501, 785, 1584
Alw26I GTCTC 6 cut(s) 413, 1309, 1526, 1691, 1743, 1913
AlwNI CAGNNNCTG 1 cut(s) 1107
Ama87I CYCGRG 2 cut(s) 317, 1916
AoxI GGCC 9 cut(s) 455, 884, 1186, 1533, 1775, 1822, 2010, 2282, 2376
ApeKI GCWGC 5 cut(s) 122, 125, 350, 2360, 2447
ApoI RAATTY 3 cut(s) 1057, 1243, 2199
AsiGI ACCGGT 2 cut(s) 218, 1925
Asp700I GAANNNNTTC 1 cut(s) 1791
AspLEI GCGC 1 cut(s) 182
AsuC2I CCSGG 4 cut(s) 318, 319, 1254, 2371
AsuNHI GCTAGC 1 cut(s) 983
AvaI CYCGRG 2 cut(s) 317, 1916
AvaII GGWCC 7 cut(s) 771, 931, 1070, 1121, 1238, 1913, 1928
AxyI CCTNAGG 1 cut(s) 1797
BaeGI GKGCMC 2 cut(s) 324, 2221
BaeI ACNNNNGTAYC 2 cut(s) 2171, 2204
BalI TGGCCA 1 cut(s) 1535
BamHI GGATCC 1 cut(s) 2438
BanII GRGCYC 1 cut(s) 501
BarI GAAGNNNNNNTAC 2 cut(s) 1256, 1288
BbsI GAAGAC 1 cut(s) 1784
Bbv12I GWGCWC 5 cut(s) 376, 429, 501, 785, 1584
BbvCI CCTCAGC 1 cut(s) 1863
BbvI GCAGC 5 cut(s) 109, 112, 337, 2347, 2434
BceAI ACGGC 2 cut(s) 1173, 1704
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BciT130I CCWGG 4 cut(s) 503, 973, 2286, 2444
BclI TGATCA 4 cut(s) 85, 865, 1081, 2398
BcnI CCSGG 4 cut(s) 318, 319, 1254, 2371
BcoDI GTCTC 6 cut(s) 413, 1309, 1526, 1691, 1743, 1913
BfaI CTAG 1 cut(s) 984
BfmI CTRYAG 1 cut(s) 2233
BfuAI ACCTGC 2 cut(s) 806, 863
BglII AGATCT 2 cut(s) 1617, 1854
BisI GCNGC 5 cut(s) 123, 126, 351, 2361, 2448
BlsI GCNGC 5 cut(s) 124, 127, 352, 2362, 2449
Bme1390I CCNGG 8 cut(s) 318, 319, 503, 973, 1254, 2286, 2371, 2444
Bme18I GGWCC 7 cut(s) 771, 931, 1070, 1121, 1238, 1913, 1928
BmeRI GACNNNNNGTC 1 cut(s) 1926
BmeT110I CYCGRG 2 cut(s) 317, 1916
BmiI GGNNCC 7 cut(s) 1071, 1239, 1778, 1915, 1930, 2003, 2440
BmrFI CCNGG 8 cut(s) 318, 319, 503, 973, 1254, 2286, 2371, 2444
BmsI GCATC 6 cut(s) 476, 1411, 1502, 1618, 2080, 2265
BmtI GCTAGC 1 cut(s) 987
BpiI GAAGAC 1 cut(s) 1784
BplI GAGNNNNNCTC 2 cut(s) 1723, 1755
BpmI CTGGAG 3 cut(s) 485, 1596, 2193
Bpu10I CCTNAGC 1 cut(s) 1863
BpuEI CTTGAG 3 cut(s) 515, 1634, 1802
BpuMI CCSGG 4 cut(s) 318, 319, 1254, 2371
BsaI GGTCTC 2 cut(s) 1526, 1913
BsaJI CCNNGG 4 cut(s) 316, 317, 2168, 2442
BsaWI WCCGGW 2 cut(s) 218, 1925
BsaXI ACNNNNNCTCC 6 cut(s) 363, 393, 2100, 2130, 2399, 2429
Bsc4I CCNNNNNNNGG 5 cut(s) 590, 617, 1486, 2104, 2169
Bse118I RCCGGY 3 cut(s) 218, 785, 1925
Bse1I ACTGG 3 cut(s) 1480, 2005, 2183
Bse21I CCTNAGG 1 cut(s) 1797
Bse3DI GCAATG 1 cut(s) 135
BseBI CCWGG 4 cut(s) 503, 973, 2286, 2444
BseDI CCNNGG 4 cut(s) 316, 317, 2168, 2442
BseGI GGATG 5 cut(s) 171, 1311, 1339, 1426, 2466
BseLI CCNNNNNNNGG 5 cut(s) 590, 617, 1486, 2104, 2169
BseMI GCAATG 1 cut(s) 135
BseMII CTCAG 8 cut(s) 408, 648, 1650, 1788, 1854, 2165, 2345, 2502
BseNI ACTGG 3 cut(s) 1480, 2005, 2183
BseRI GAGGAG 3 cut(s) 1583, 1880, 2285
BseSI GKGCMC 2 cut(s) 324, 2221
BseXI GCAGC 5 cut(s) 109, 112, 337, 2347, 2434
BseYI CCCAGC 2 cut(s) 754, 1997
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 9 cut(s) 457, 886, 1188, 1535, 1777, 1824, 2012, 2284, 2378
BshTI ACCGGT 2 cut(s) 218, 1925
BsiHKAI GWGCWC 5 cut(s) 376, 429, 501, 785, 1584
BsiHKCI CYCGRG 2 cut(s) 317, 1916
BsiSI CCGG 6 cut(s) 219, 318, 786, 1254, 1926, 2370
BslFI GGGAC 3 cut(s) 1892, 1899, 1914
BslI CCNNNNNNNGG 5 cut(s) 590, 617, 1486, 2104, 2169
BsmAI GTCTC 6 cut(s) 413, 1309, 1526, 1691, 1743, 1913
BsmFI GGGAC 3 cut(s) 1892, 1899, 1914
BsmI GAATGC 2 cut(s) 556, 1396
BsnI GGCC 9 cut(s) 457, 886, 1188, 1535, 1777, 1824, 2012, 2284, 2378
Bso31I GGTCTC 2 cut(s) 1526, 1913
BsoBI CYCGRG 2 cut(s) 317, 1916
Bsp1286I GDGCHC 7 cut(s) 324, 376, 429, 501, 785, 1584, 2221
Bsp1407I TGTACA 1 cut(s) 1761
BspACI CCGC 5 cut(s) 128, 1155, 1344, 2226, 2504
BspANI GGCC 9 cut(s) 457, 886, 1188, 1535, 1777, 1824, 2012, 2284, 2378
BspCNI CTCAG 8 cut(s) 409, 647, 1651, 1789, 1855, 2164, 2346, 2503
BspFNI CGCG 1 cut(s) 180
BspLI GGNNCC 7 cut(s) 1071, 1239, 1778, 1915, 1930, 2003, 2440
BspMI ACCTGC 2 cut(s) 806, 863
BspOI GCTAGC 1 cut(s) 987
BspTNI GGTCTC 2 cut(s) 1526, 1913
BsrBI CCGCTC 1 cut(s) 2506
BsrDI GCAATG 1 cut(s) 135
BsrFI RCCGGY 3 cut(s) 218, 785, 1925
BsrGI TGTACA 1 cut(s) 1761
BsrI ACTGG 3 cut(s) 1480, 2005, 2183
BssAI RCCGGY 3 cut(s) 218, 785, 1925
BssECI CCNNGG 4 cut(s) 316, 317, 2168, 2442
BssT1I CCWWGG 1 cut(s) 2168
Bst2UI CCWGG 4 cut(s) 503, 973, 2286, 2444
Bst6I CTCTTC 1 cut(s) 911
BstAUI TGTACA 1 cut(s) 1761
BstC8I GCNNGC 3 cut(s) 803, 985, 2376
BstEII GGTNACC 1 cut(s) 975
BstF5I GGATG 5 cut(s) 171, 1311, 1339, 1426, 2466
BstFNI CGCG 1 cut(s) 180
BstHHI GCGC 1 cut(s) 182
BstMAI GTCTC 6 cut(s) 413, 1309, 1526, 1691, 1743, 1913
BstMWI GCNNNNNNNGC 4 cut(s) 188, 635, 964, 989
BstNI CCWGG 4 cut(s) 503, 973, 2286, 2444
BstNSI RCATGY 2 cut(s) 1951, 2264
BstPI GGTNACC 1 cut(s) 975
BstSCI CCNGG 8 cut(s) 316, 317, 501, 971, 1252, 2284, 2369, 2442
BstSFI CTRYAG 1 cut(s) 2233
BstSLI GKGCMC 2 cut(s) 324, 2221
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 5 cut(s) 109, 112, 337, 2347, 2434
BstV2I GAAGAC 1 cut(s) 1784
BstX2I RGATCY 6 cut(s) 580, 1617, 1630, 1854, 1885, 2438
BstXI CCANNNNNNTGG 3 cut(s) 509, 1858, 1939
BstYI RGATCY 6 cut(s) 580, 1617, 1630, 1854, 1885, 2438
Bsu36I CCTNAGG 1 cut(s) 1797
BsuRI GGCC 9 cut(s) 457, 886, 1188, 1535, 1777, 1824, 2012, 2284, 2378
BtsCI GGATG 5 cut(s) 171, 1311, 1339, 1426, 2466
BtsI GCAGTG 2 cut(s) 789, 987
BtsIMutI CAGTG 8 cut(s) 789, 987, 1350, 1487, 1731, 2012, 2190, 2242
BveI ACCTGC 2 cut(s) 806, 863
Cac8I GCNNGC 3 cut(s) 803, 985, 2376
CaiI CAGNNNCTG 1 cut(s) 1107
CfoI GCGC 1 cut(s) 182
Cfr10I RCCGGY 3 cut(s) 218, 785, 1925
Cfr9I CCCGGG 1 cut(s) 317
CsiI ACCWGGT 1 cut(s) 971
CspAI ACCGGT 2 cut(s) 218, 1925
DrdI GACNNNNNNGTC 1 cut(s) 250
DriI GACNNNNNGTC 1 cut(s) 1926
DseDI GACNNNNNNGTC 1 cut(s) 250
EaeI YGGCCR 2 cut(s) 1186, 1533
Eam1104I CTCTTC 1 cut(s) 911
Eam1105I GACNNNNNGTC 1 cut(s) 1926
EarI CTCTTC 1 cut(s) 911
Ecl136II GAGCTC 1 cut(s) 499
Eco130I CCWWGG 1 cut(s) 2168
Eco147I AGGCCT 2 cut(s) 457, 2378
Eco24I GRGCYC 1 cut(s) 501
Eco31I GGTCTC 2 cut(s) 1526, 1913
Eco47I GGWCC 7 cut(s) 771, 931, 1070, 1121, 1238, 1913, 1928
Eco53kI GAGCTC 1 cut(s) 499
Eco57I CTGAAG 1 cut(s) 360
Eco81I CCTNAGG 1 cut(s) 1797
Eco88I CYCGRG 2 cut(s) 317, 1916
Eco91I GGTNACC 1 cut(s) 975
EcoICRI GAGCTC 1 cut(s) 499
EcoO109I RGGNCCY 3 cut(s) 931, 1070, 1776
EcoO65I GGTNACC 1 cut(s) 975
EcoRII CCWGG 4 cut(s) 501, 971, 2284, 2442
EcoT14I CCWWGG 1 cut(s) 2168
EcoT38I GRGCYC 1 cut(s) 501
ErhI CCWWGG 1 cut(s) 2168
FalI AAGNNNNNCTT 2 cut(s) 570, 602
FaqI GGGAC 3 cut(s) 1892, 1899, 1914
FauI CCCGC 3 cut(s) 121, 1162, 2511
FauNDI CATATG 1 cut(s) 574
FbaI TGATCA 4 cut(s) 85, 865, 1081, 2398
FblI GTMKAC 1 cut(s) 1944
Fnu4HI GCNGC 5 cut(s) 123, 126, 351, 2361, 2448
FokI GGATG 5 cut(s) 178, 1318, 1346, 1433, 2453
FriOI GRGCYC 1 cut(s) 501
Fsp4HI GCNGC 5 cut(s) 123, 126, 351, 2361, 2448
FspBI CTAG 1 cut(s) 984
GlaI GCGC 1 cut(s) 181
GluI GCNGC 5 cut(s) 123, 126, 351, 2361, 2448
GsaI CCCAGC 2 cut(s) 758, 2001
GsuI CTGGAG 3 cut(s) 485, 1596, 2193
HaeIII GGCC 9 cut(s) 457, 886, 1188, 1535, 1777, 1824, 2012, 2284, 2378
HapII CCGG 6 cut(s) 219, 318, 786, 1254, 1926, 2370
HhaI GCGC 1 cut(s) 182
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HincII GTYRAC 7 cut(s) 99, 328, 472, 894, 1032, 1945, 2351
HindII GTYRAC 7 cut(s) 99, 328, 472, 894, 1032, 1945, 2351
HindIII AAGCTT 2 cut(s) 519, 899
HpaI GTTAAC 1 cut(s) 472
HpaII CCGG 6 cut(s) 219, 318, 786, 1254, 1926, 2370
Hpy99I CGWCG 1 cut(s) 2327
HpyCH4IV ACGT 5 cut(s) 324, 1275, 1284, 1528, 2254
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 635, 964, 989
HpySE526I ACGT 5 cut(s) 324, 1275, 1284, 1528, 2254
HspAI GCGC 1 cut(s) 180
Ksp22I TGATCA 4 cut(s) 85, 865, 1081, 2398
KspAI GTTAAC 1 cut(s) 472
LmnI GCTCC 6 cut(s) 371, 432, 504, 1565, 2212, 2491
Lsp1109I GCAGC 5 cut(s) 109, 112, 337, 2347, 2434
LweI GCATC 6 cut(s) 476, 1411, 1502, 1618, 2080, 2265
MabI ACCWGGT 1 cut(s) 971
MaeI CTAG 1 cut(s) 984
MaeII ACGT 5 cut(s) 324, 1275, 1284, 1528, 2254
MaeIII GTNAC 7 cut(s) 343, 824, 907, 975, 1109, 1346, 2458
MbiI CCGCTC 1 cut(s) 2506
MboII GAAGA 8 cut(s) 32, 590, 775, 928, 1273, 1784, 2048, 2072
MflI RGATCY 6 cut(s) 580, 1617, 1630, 1854, 1885, 2438
MhlI GDGCHC 7 cut(s) 324, 376, 429, 501, 785, 1584, 2221
MlsI TGGCCA 1 cut(s) 1535
MluCI AATT 4 cut(s) 207, 1057, 1243, 2199
MluNI TGGCCA 1 cut(s) 1535
MlyI GAGTC 5 cut(s) 104, 260, 719, 1580, 2339
MmeI TCCRAC 1 cut(s) 2147
Mox20I TGGCCA 1 cut(s) 1535
MroXI GAANNNNTTC 1 cut(s) 1791
MscI TGGCCA 1 cut(s) 1535
MseI TTAA 5 cut(s) 210, 471, 1278, 1400, 1971
MslI CAYNNNNRTG 2 cut(s) 857, 2088
Msp20I TGGCCA 1 cut(s) 1535
MspA1I CMGCKG 2 cut(s) 350, 638
MspI CCGG 6 cut(s) 219, 318, 786, 1254, 1926, 2370
MspR9I CCNGG 8 cut(s) 318, 319, 503, 973, 1254, 2286, 2371, 2444
Mva1269I GAATGC 2 cut(s) 556, 1396
MvaI CCWGG 4 cut(s) 503, 973, 2286, 2444
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 4 cut(s) 188, 635, 964, 989
NciI CCSGG 4 cut(s) 318, 319, 1254, 2371
NdeI CATATG 1 cut(s) 574
NheI GCTAGC 1 cut(s) 983
NlaIV GGNNCC 7 cut(s) 1071, 1239, 1778, 1915, 1930, 2003, 2440
NmuCI GTSAC 4 cut(s) 343, 907, 1109, 2458
NspI RCATGY 2 cut(s) 1951, 2264
PaqCI CACCTGC 1 cut(s) 863
PceI AGGCCT 2 cut(s) 457, 2378
PciI ACATGT 1 cut(s) 1947
PcsI WCGNNNNNNNCGW 1 cut(s) 1281
PctI GAATGC 2 cut(s) 556, 1396
PdmI GAANNNNTTC 1 cut(s) 1791
PfeI GAWTC 8 cut(s) 311, 1150, 1173, 1230, 1843, 1985, 2245, 2340
PflMI CCANNNNNTGG 1 cut(s) 590
PfoI TCCNGGA 1 cut(s) 1252
PinAI ACCGGT 2 cut(s) 218, 1925
PkrI GCNGC 5 cut(s) 124, 127, 352, 2362, 2449
PleI GAGTC 5 cut(s) 104, 259, 718, 1579, 2338
PpsI GAGTC 5 cut(s) 104, 259, 718, 1579, 2338
PpuMI RGGWCCY 2 cut(s) 931, 1070
PscI ACATGT 1 cut(s) 1947
PsiI TTATAA 1 cut(s) 1667
Psp124BI GAGCTC 1 cut(s) 501
Psp1406I AACGTT 1 cut(s) 1528
Psp5II RGGWCCY 2 cut(s) 931, 1070
Psp6I CCWGG 4 cut(s) 501, 971, 2284, 2442
PspEI GGTNACC 1 cut(s) 975
PspFI CCCAGC 2 cut(s) 754, 1997
PspGI CCWGG 4 cut(s) 501, 971, 2284, 2442
PspN4I GGNNCC 7 cut(s) 1071, 1239, 1778, 1915, 1930, 2003, 2440
PspPPI RGGWCCY 2 cut(s) 931, 1070
PstNI CAGNNNCTG 1 cut(s) 1107
PsuI RGATCY 6 cut(s) 580, 1617, 1630, 1854, 1885, 2438
PvuII CAGCTG 2 cut(s) 350, 638
RseI CAYNNNNRTG 2 cut(s) 857, 2088
SacI GAGCTC 1 cut(s) 501
SalI GTCGAC 1 cut(s) 1943
SaqAI TTAA 5 cut(s) 210, 471, 1278, 1400, 1971
SatI GCNGC 5 cut(s) 123, 126, 351, 2361, 2448
SchI GAGTC 5 cut(s) 104, 260, 719, 1580, 2339
ScrFI CCNGG 8 cut(s) 318, 319, 503, 973, 1254, 2286, 2371, 2444
SduI GDGCHC 7 cut(s) 324, 376, 429, 501, 785, 1584, 2221
SexAI ACCWGGT 1 cut(s) 971
SfaNI GCATC 6 cut(s) 476, 1411, 1502, 1618, 2080, 2265
SfcI CTRYAG 1 cut(s) 2233
SinI GGWCC 7 cut(s) 771, 931, 1070, 1121, 1238, 1913, 1928
SmaI CCCGGG 1 cut(s) 319
SmiMI CAYNNNNRTG 2 cut(s) 857, 2088
SmlI CTYRAG 3 cut(s) 494, 1613, 1781
SmoI CTYRAG 3 cut(s) 494, 1613, 1781
Sse9I AATT 4 cut(s) 207, 1057, 1243, 2199
SseBI AGGCCT 2 cut(s) 457, 2378
SsiI CCGC 5 cut(s) 128, 1155, 1344, 2226, 2504
SspMI CTAG 1 cut(s) 984
SstI GAGCTC 1 cut(s) 501
StuI AGGCCT 2 cut(s) 457, 2378
StyD4I CCNGG 8 cut(s) 316, 317, 501, 971, 1252, 2284, 2369, 2442
StyI CCWWGG 1 cut(s) 2168
TaiI ACGT 5 cut(s) 327, 1278, 1287, 1531, 2257
TaqI TCGA 3 cut(s) 1944, 2178, 2517
TaqII GACCGA 1 cut(s) 1355
TasI AATT 4 cut(s) 207, 1057, 1243, 2199
TatI WGTACW 2 cut(s) 231, 1761
TfiI GAWTC 8 cut(s) 311, 1150, 1173, 1230, 1843, 1985, 2245, 2340
Tru1I TTAA 5 cut(s) 210, 471, 1278, 1400, 1971
Tru9I TTAA 5 cut(s) 210, 471, 1278, 1400, 1971
TscAI CASTG 8 cut(s) 796, 994, 1357, 1487, 1731, 2012, 2190, 2242
TseFI GTSAC 4 cut(s) 343, 907, 1109, 2458
TseI GCWGC 5 cut(s) 122, 125, 350, 2360, 2447
Tsp45I GTSAC 4 cut(s) 343, 907, 1109, 2458
TspDTI ATGAA 8 cut(s) 324, 591, 875, 929, 968, 1457, 1824, 1989
TspGWI ACGGA 8 cut(s) 17, 297, 419, 1017, 1424, 1441, 1682, 2459
TspMI CCCGGG 1 cut(s) 317
TspRI CASTG 8 cut(s) 796, 994, 1357, 1487, 1731, 2012, 2190, 2242
Van91I CCANNNNNTGG 1 cut(s) 590
VpaK11BI GGWCC 7 cut(s) 771, 931, 1070, 1121, 1238, 1913, 1928
XapI RAATTY 3 cut(s) 1057, 1243, 2199
XceI RCATGY 2 cut(s) 1951, 2264
XmaI CCCGGG 1 cut(s) 317
XmiI GTMKAC 1 cut(s) 1944
XmnI GAANNNNTTC 1 cut(s) 1791
XspI CTAG 1 cut(s) 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.