pycom10g17380

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001995
Physical Location & Seq
Reverse (-)
20577454 .. 20579223
1770 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g17380.1

Sequence Viewer

Length: 1770 bp
ATGGACCAGCAAGAGCACGGGCACTGCTACCTGCAAGCGTGGTTTTGTTCAGTTCTGTTACGAACCCATCAAGCAGATCGATCATGGCACACCTGCATGAATGATCAGAAGGATAAGTTGTGGCCCATGTTGACAAAGCTTGGTGTCACCATGAAGAGTGATGAGAAGGACCTGATGGGGAAGGCTCTGATGAAGCGTGTTATGCAGACCTGGCTACCAGCTAGCACTGCTTTATTGGAAATGATGATTTTTCACCTTCCATCTCCGTCAACGGCTCAAAAGTACCGTGTTGAGAATTTGTACGAGGGTCCTCTTGATGATCAATATGCCAATGCCATCAGGAACTGTGACCCTGATGGACCTCTTATGCTCTATGTGTCCAAGATGATTCCCGCTTCTGATAAGGGTAGATTCTTTGCCTTTGGCCGTGTCTTTGCTGGTAGAGTTCAGACAGGATTGAAGGTTAGAATCATGGGTCCAAATTTTGTTCCTGGAGAGAAGAAGGATTTGTATGTTAAGAACGTACAGAGAACTGTTATTTGGATGGGAAAGAGACAAGAAACTGTTGAGGATGTTCCTTGCGGTAACACTGTGGCATTGGTTGGTCTGGATCAGTTTATTACCAAGAATGCAACTTTAACTAATGAGAAGGAAGTGGATGCTCACCCTATCCGTGCTATGAAGTTCTCCGTTTCACCTGTCGTGCGTGTTGCTGTCCAGTGCAAGGTTGCATCTGACCTTCCCAAACTTGTTGAAGGTCTCAAACGTTTGGCCAAGTCTGATCCTATGGTTGTCTGCTCCATTGAGGAGTCTGGAGAGCACATTATTGCTGGTGCTGGTGAACTGCATCTTGAGATCTGTTTGAAGGATCTGCAAGATGATTTTATGGGTGGTGCTGAGATTATAAAGTCTGACCCCGTTGTGTCCTTCCGTGAGACTGTCCTTGATAAGTCCTGCCGCACAGTGATGAGCAAGTCTCCAAACAAGCATAACCGTCTGTACATGGAAGCAAGGCCCCTTGAGGAAGGTCTTCCCGAGGCTATTGATGAAGGTCGCATTGGCCCAAGAGATGATCCCAAGATTCGTTCCAAGATCTTGGCTGAGGAGTTTGGTTGGGACAAGGATCTTGCTAAGAAAATCTGGTGTTTTGGTCCCGAGACCACTGGTCCCAACATGGTGGTTGACATGTGTAAGGGAGTTCAGTACCTTAATGAAATCAAGGATTCGGTTGTTGCTGGGTTCCAGTGGGCCTCAAAGGAAGGTGCATTGGCAGAAGAAAACATGAGAGGTATTTGCTTTGAAGTCTGTGATGTGGTTCTTCACGCTGATGCTATCCACAGAGGGGGAGGTCAGGTCATTCCAACTGCTCGGAGGGTCATCTATGCCTCTCAGCTCACAGCCAAGCCAAGGCTTCTCGAACCAGTGTATCTCGTTGAAATTCAAGCTCCAGAGGGGGCACTTGGCGGGATCTACAGTGTTCTGAATCAGAAACGTGGGCATGTGTTTGAGGAGATGCAGAGGCCAGGAACTCCACTTTACAACATCAAGGCATACCTCCCCGTCGTTGAGTCCTTTGGATTCTCTGGTCAACTGAGGGCTGCAACGTCTGGGCAGGCTTTCCCACAATGCGTGTTTGATCATTGGGAGATGATGTCGTCTGATCCATTGGAAGCTGGATCCCAGGCTGCAACACTTGTCACCGACATCCGTAAGAGGAAGGGTTTGAAGGAGCAGATGACCCCGCTCTCTGAGTTCGAGGACAAGCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04152 ko04921 map04152 map04921
Pfam Domains
Protein Families
EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

590

Amino Acids

65.88

Weight (kDa)

6.39

Isoelectric Point (pI)

35.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-G_D2 PF22042 125 - 215 3.3e-06 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 140 - 208 6.9e-08 Elongation factor Tu domain 2
EFG_III PF14492 233 - 295 6.3e-12 Elongation Factor G, domain III
EFG_IV PF03764 356 - 468 1.2e-29 Elongation factor G, domain IV
EFG_C PF00679 470 - 557 1.2e-20 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 905
AarI CACCTGC 1 cut(s) 101
Acc36I ACCTGC 2 cut(s) 39, 101
AccBSI CCGCTC 1 cut(s) 1744
AciI CCGC 5 cut(s) 393, 582, 958, 1464, 1742
AclI AACGTT 1 cut(s) 766
AclWI GGATC 9 cut(s) 618, 776, 876, 1067, 1131, 1475, 1655, 1671, 1684
AcoI YGGCCR 2 cut(s) 424, 771
AcsI RAATTY 3 cut(s) 295, 481, 1437
AfaI GTAC 5 cut(s) 284, 302, 525, 1001, 1205
AfiI CCNNNNNNNGG 3 cut(s) 724, 1342, 1407
AflIII ACRYGT 1 cut(s) 1185
AgsI TTSAA 7 cut(s) 460, 755, 865, 1301, 1436, 1442, 1726
AhdI GACNNNNNGTC 1 cut(s) 1164
AjnI CCWGG 4 cut(s) 209, 490, 1522, 1680
AjuI GAANNNNNNNTTGG 4 cut(s) 523, 555, 1041, 1073
AluBI AGCT 6 cut(s) 139, 221, 1393, 1445, 1673, 1765
AluI AGCT 6 cut(s) 139, 221, 1393, 1445, 1673, 1765
Alw21I GWGCWC 2 cut(s) 18, 822
Alw26I GTCTC 5 cut(s) 547, 764, 929, 981, 1151
AlwI GGATC 9 cut(s) 618, 776, 876, 1067, 1131, 1475, 1655, 1671, 1684
AlwNI CAGNNNCTG 1 cut(s) 345
Ama87I CYCGRG 2 cut(s) 1034, 1154
AoxI GGCC 7 cut(s) 122, 424, 771, 1013, 1060, 1248, 1520
ApeKI GCWGC 2 cut(s) 1598, 1685
ApoI RAATTY 3 cut(s) 295, 481, 1437
Asp700I GAANNNNTTC 1 cut(s) 1029
AsuHPI GGTGA 6 cut(s) 139, 245, 656, 687, 851, 1690
AsuNHI GCTAGC 1 cut(s) 221
AvaI CYCGRG 2 cut(s) 1034, 1154
AvaII GGWCC 7 cut(s) 4, 169, 308, 359, 476, 1151, 1166
BaeGI GKGCMC 2 cut(s) 24, 1459
BaeI ACNNNNGTAYC 2 cut(s) 1409, 1442
BalI TGGCCA 1 cut(s) 773
BamHI GGATCC 1 cut(s) 1676
BbsI GAAGAC 1 cut(s) 1022
Bbv12I GWGCWC 2 cut(s) 18, 822
BbvCI CCTCAGC 1 cut(s) 1101
BbvI GCAGC 2 cut(s) 1585, 1672
BccI CCATC 6 cut(s) 75, 169, 268, 344, 350, 538
BceAI ACGGC 2 cut(s) 288, 411
BciT130I CCWGG 4 cut(s) 211, 492, 1524, 1682
BclI TGATCA 3 cut(s) 103, 319, 1636
BcoDI GTCTC 5 cut(s) 547, 764, 929, 981, 1151
BfaI CTAG 1 cut(s) 222
BfmI CTRYAG 1 cut(s) 1471
BfuAI ACCTGC 2 cut(s) 39, 101
BglII AGATCT 2 cut(s) 855, 1092
BisI GCNGC 3 cut(s) 958, 1599, 1686
BlsI GCNGC 3 cut(s) 959, 1600, 1687
Bme1390I CCNGG 4 cut(s) 211, 492, 1524, 1682
Bme18I GGWCC 7 cut(s) 4, 169, 308, 359, 476, 1151, 1166
BmeRI GACNNNNNGTC 1 cut(s) 1164
BmeT110I CYCGRG 2 cut(s) 1034, 1154
BmiI GGNNCC 7 cut(s) 309, 477, 1016, 1153, 1168, 1241, 1678
BmrFI CCNGG 4 cut(s) 211, 492, 1524, 1682
BmsI GCATC 5 cut(s) 649, 740, 856, 1318, 1503
BmtI GCTAGC 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 1022
BplI GAGNNNNNCTC 2 cut(s) 961, 993
BpmI CTGGAG 3 cut(s) 513, 834, 1431
Bpu10I CCTNAGC 1 cut(s) 1101
BpuEI CTTGAG 2 cut(s) 872, 1040
Bsa29I ATCGAT 1 cut(s) 79
BsaI GGTCTC 2 cut(s) 764, 1151
BsaJI CCNNGG 3 cut(s) 1035, 1406, 1680
BsaXI ACNNNNNCTCC 4 cut(s) 1338, 1368, 1637, 1667
Bsc4I CCNNNNNNNGG 3 cut(s) 724, 1342, 1407
Bse1I ACTGG 4 cut(s) 718, 1168, 1243, 1421
BseBI CCWGG 4 cut(s) 211, 492, 1524, 1682
BseCI ATCGAT 1 cut(s) 79
BseDI CCNNGG 3 cut(s) 1035, 1406, 1680
BseGI GGATG 4 cut(s) 549, 577, 664, 1704
BseLI CCNNNNNNNGG 3 cut(s) 724, 1342, 1407
BseMII CTCAG 5 cut(s) 888, 1092, 1403, 1583, 1740
BseNI ACTGG 4 cut(s) 718, 1168, 1243, 1421
BseRI GAGGAG 3 cut(s) 821, 1118, 1523
BseSI GKGCMC 2 cut(s) 24, 1459
BseXI GCAGC 2 cut(s) 1585, 1672
BseYI CCCAGC 1 cut(s) 1235
BshFI GGCC 7 cut(s) 124, 426, 773, 1015, 1062, 1250, 1522
BshVI ATCGAT 1 cut(s) 79
BsiHKAI GWGCWC 2 cut(s) 18, 822
BsiHKCI CYCGRG 2 cut(s) 1034, 1154
BslFI GGGAC 3 cut(s) 1130, 1137, 1152
BslI CCNNNNNNNGG 3 cut(s) 724, 1342, 1407
BsmAI GTCTC 5 cut(s) 547, 764, 929, 981, 1151
BsmFI GGGAC 3 cut(s) 1130, 1137, 1152
BsmI GAATGC 1 cut(s) 634
BsnI GGCC 7 cut(s) 124, 426, 773, 1015, 1062, 1250, 1522
Bso31I GGTCTC 2 cut(s) 764, 1151
BsoBI CYCGRG 2 cut(s) 1034, 1154
Bsp1286I GDGCHC 4 cut(s) 18, 24, 822, 1459
Bsp1407I TGTACA 1 cut(s) 999
BspACI CCGC 5 cut(s) 393, 582, 958, 1464, 1742
BspANI GGCC 7 cut(s) 124, 426, 773, 1015, 1062, 1250, 1522
BspCNI CTCAG 5 cut(s) 889, 1093, 1402, 1584, 1741
BspDI ATCGAT 1 cut(s) 79
BspLI GGNNCC 7 cut(s) 309, 477, 1016, 1153, 1168, 1241, 1678
BspMI ACCTGC 2 cut(s) 39, 101
BspOI GCTAGC 1 cut(s) 225
BspPI GGATC 9 cut(s) 618, 776, 876, 1067, 1131, 1475, 1655, 1671, 1684
BspTNI GGTCTC 2 cut(s) 764, 1151
BsrBI CCGCTC 1 cut(s) 1744
BsrGI TGTACA 1 cut(s) 999
BsrI ACTGG 4 cut(s) 718, 1168, 1243, 1421
BssECI CCNNGG 3 cut(s) 1035, 1406, 1680
BssT1I CCWWGG 1 cut(s) 1406
Bst2UI CCWGG 4 cut(s) 211, 492, 1524, 1682
Bst4CI ACNGT 9 cut(s) 287, 347, 535, 565, 592, 940, 964, 995, 1475
Bst6I CTCTTC 1 cut(s) 149
BstAUI TGTACA 1 cut(s) 999
BstC8I GCNNGC 3 cut(s) 36, 223, 1614
BstDEI CTNAG 6 cut(s) 897, 1101, 1131, 1389, 1592, 1749
BstF5I GGATG 4 cut(s) 549, 577, 664, 1704
BstMAI GTCTC 5 cut(s) 547, 764, 929, 981, 1151
BstMWI GCNNNNNNNGC 3 cut(s) 202, 211, 227
BstNI CCWGG 4 cut(s) 211, 492, 1524, 1682
BstNSI RCATGY 2 cut(s) 1189, 1502
BstSCI CCNGG 4 cut(s) 209, 490, 1522, 1680
BstSFI CTRYAG 1 cut(s) 1471
BstSLI GKGCMC 2 cut(s) 24, 1459
BstV1I GCAGC 2 cut(s) 1585, 1672
BstV2I GAAGAC 1 cut(s) 1022
BstX2I RGATCY 6 cut(s) 855, 868, 1092, 1123, 1467, 1676
BstXI CCANNNNNNTGG 2 cut(s) 1096, 1177
BstYI RGATCY 6 cut(s) 855, 868, 1092, 1123, 1467, 1676
Bsu15I ATCGAT 1 cut(s) 79
BsuRI GGCC 7 cut(s) 124, 426, 773, 1015, 1062, 1250, 1522
BsuTUI ATCGAT 1 cut(s) 79
BtsCI GGATG 4 cut(s) 549, 577, 664, 1704
BtsI GCAGTG 2 cut(s) 22, 225
BtsIMutI CAGTG 9 cut(s) 22, 225, 588, 725, 969, 1161, 1250, 1428, 1480
BveI ACCTGC 2 cut(s) 39, 101
Cac8I GCNNGC 3 cut(s) 36, 223, 1614
CaiI CAGNNNCTG 1 cut(s) 345
ClaI ATCGAT 1 cut(s) 79
Csp6I GTAC 5 cut(s) 283, 301, 524, 1000, 1204
CviQI GTAC 5 cut(s) 283, 301, 524, 1000, 1204
DdeI CTNAG 6 cut(s) 897, 1101, 1131, 1389, 1592, 1749
DriI GACNNNNNGTC 1 cut(s) 1164
EaeI YGGCCR 2 cut(s) 424, 771
Eam1104I CTCTTC 1 cut(s) 149
Eam1105I GACNNNNNGTC 1 cut(s) 1164
EarI CTCTTC 1 cut(s) 149
Eco130I CCWWGG 1 cut(s) 1406
Eco31I GGTCTC 2 cut(s) 764, 1151
Eco47I GGWCC 7 cut(s) 4, 169, 308, 359, 476, 1151, 1166
Eco88I CYCGRG 2 cut(s) 1034, 1154
EcoO109I RGGNCCY 3 cut(s) 169, 308, 1014
EcoRII CCWGG 4 cut(s) 209, 490, 1522, 1680
EcoT14I CCWWGG 1 cut(s) 1406
ErhI CCWWGG 1 cut(s) 1406
FaqI GGGAC 3 cut(s) 1130, 1137, 1152
FauI CCCGC 3 cut(s) 400, 1457, 1749
FbaI TGATCA 3 cut(s) 103, 319, 1636
Fnu4HI GCNGC 3 cut(s) 958, 1599, 1686
FokI GGATG 4 cut(s) 556, 584, 671, 1691
Fsp4HI GCNGC 3 cut(s) 958, 1599, 1686
FspBI CTAG 1 cut(s) 222
GluI GCNGC 3 cut(s) 958, 1599, 1686
GsaI CCCAGC 1 cut(s) 1239
GsuI CTGGAG 3 cut(s) 513, 834, 1431
HaeIII GGCC 7 cut(s) 124, 426, 773, 1015, 1062, 1250, 1522
HincII GTYRAC 4 cut(s) 132, 270, 1183, 1589
HindII GTYRAC 4 cut(s) 132, 270, 1183, 1589
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 9 cut(s) 388, 411, 468, 809, 1081, 1223, 1483, 1568, 1578
HphI GGTGA 6 cut(s) 139, 245, 656, 687, 851, 1690
Hpy166II GTNNAC 5 cut(s) 132, 270, 842, 1183, 1589
Hpy188III TCNNGA 9 cut(s) 314, 340, 608, 813, 851, 1034, 1154, 1415, 1448
Hpy8I GTNNAC 5 cut(s) 132, 270, 842, 1183, 1589
Hpy99I CGWCG 1 cut(s) 1565
HpyCH4III ACNGT 9 cut(s) 287, 347, 535, 565, 592, 940, 964, 995, 1475
HpyCH4IV ACGT 4 cut(s) 522, 766, 1492, 1604
HpyF10VI GCNNNNNNNGC 3 cut(s) 202, 211, 227
HpyF3I CTNAG 6 cut(s) 897, 1101, 1131, 1389, 1592, 1749
HpySE526I ACGT 4 cut(s) 522, 766, 1492, 1604
Ksp22I TGATCA 3 cut(s) 103, 319, 1636
LmnI GCTCC 3 cut(s) 803, 1450, 1729
Lsp1109I GCAGC 2 cut(s) 1585, 1672
LweI GCATC 5 cut(s) 649, 740, 856, 1318, 1503
MaeI CTAG 1 cut(s) 222
MaeII ACGT 4 cut(s) 522, 766, 1492, 1604
MaeIII GTNAC 5 cut(s) 57, 145, 347, 584, 1696
MbiI CCGCTC 1 cut(s) 1744
MboII GAAGA 5 cut(s) 166, 511, 1022, 1286, 1310
MflI RGATCY 6 cut(s) 855, 868, 1092, 1123, 1467, 1676
MhlI GDGCHC 4 cut(s) 18, 24, 822, 1459
MlsI TGGCCA 1 cut(s) 773
MluCI AATT 3 cut(s) 295, 481, 1437
MluNI TGGCCA 1 cut(s) 773
MlyI GAGTC 2 cut(s) 818, 1577
MmeI TCCRAC 1 cut(s) 1385
Mox20I TGGCCA 1 cut(s) 773
MroXI GAANNNNTTC 1 cut(s) 1029
MscI TGGCCA 1 cut(s) 773
MseI TTAA 3 cut(s) 516, 638, 1209
MslI CAYNNNNRTG 3 cut(s) 95, 965, 1326
Msp20I TGGCCA 1 cut(s) 773
MspR9I CCNGG 4 cut(s) 211, 492, 1524, 1682
Mva1269I GAATGC 1 cut(s) 634
MvaI CCWGG 4 cut(s) 211, 492, 1524, 1682
MwoI GCNNNNNNNGC 3 cut(s) 202, 211, 227
NheI GCTAGC 1 cut(s) 221
NlaIV GGNNCC 7 cut(s) 309, 477, 1016, 1153, 1168, 1241, 1678
NmuCI GTSAC 3 cut(s) 145, 347, 1696
NspI RCATGY 2 cut(s) 1189, 1502
PaqCI CACCTGC 1 cut(s) 101
PciI ACATGT 1 cut(s) 1185
PctI GAATGC 1 cut(s) 634
PdmI GAANNNNTTC 1 cut(s) 1029
PfeI GAWTC 7 cut(s) 388, 411, 468, 1081, 1223, 1483, 1578
PfoI TCCNGGA 1 cut(s) 490
PkrI GCNGC 3 cut(s) 959, 1600, 1687
PleI GAGTC 2 cut(s) 817, 1576
PpsI GAGTC 2 cut(s) 817, 1576
PpuMI RGGWCCY 2 cut(s) 169, 308
PscI ACATGT 1 cut(s) 1185
PsiI TTATAA 1 cut(s) 905
Psp1406I AACGTT 1 cut(s) 766
Psp5II RGGWCCY 2 cut(s) 169, 308
Psp6I CCWGG 4 cut(s) 209, 490, 1522, 1680
PspFI CCCAGC 1 cut(s) 1235
PspGI CCWGG 4 cut(s) 209, 490, 1522, 1680
PspN4I GGNNCC 7 cut(s) 309, 477, 1016, 1153, 1168, 1241, 1678
PspPPI RGGWCCY 2 cut(s) 169, 308
PstNI CAGNNNCTG 1 cut(s) 345
PsuI RGATCY 6 cut(s) 855, 868, 1092, 1123, 1467, 1676
RsaI GTAC 5 cut(s) 284, 302, 525, 1001, 1205
RsaNI GTAC 5 cut(s) 283, 301, 524, 1000, 1204
RseI CAYNNNNRTG 3 cut(s) 95, 965, 1326
SaqAI TTAA 3 cut(s) 516, 638, 1209
SatI GCNGC 3 cut(s) 958, 1599, 1686
SchI GAGTC 2 cut(s) 818, 1577
ScrFI CCNGG 4 cut(s) 211, 492, 1524, 1682
SduI GDGCHC 4 cut(s) 18, 24, 822, 1459
SfaNI GCATC 5 cut(s) 649, 740, 856, 1318, 1503
SfcI CTRYAG 1 cut(s) 1471
SinI GGWCC 7 cut(s) 4, 169, 308, 359, 476, 1151, 1166
SmiMI CAYNNNNRTG 3 cut(s) 95, 965, 1326
SmlI CTYRAG 2 cut(s) 851, 1019
SmoI CTYRAG 2 cut(s) 851, 1019
Sse9I AATT 3 cut(s) 295, 481, 1437
SsiI CCGC 5 cut(s) 393, 582, 958, 1464, 1742
SspMI CTAG 1 cut(s) 222
StyD4I CCNGG 4 cut(s) 209, 490, 1522, 1680
StyI CCWWGG 1 cut(s) 1406
TaaI ACNGT 9 cut(s) 287, 347, 535, 565, 592, 940, 964, 995, 1475
TaiI ACGT 4 cut(s) 525, 769, 1495, 1607
TaqI TCGA 3 cut(s) 79, 1416, 1755
TasI AATT 3 cut(s) 295, 481, 1437
TatI WGTACW 1 cut(s) 999
TauI GCSGC 1 cut(s) 960
TfiI GAWTC 7 cut(s) 388, 411, 468, 1081, 1223, 1483, 1578
Tru1I TTAA 3 cut(s) 516, 638, 1209
Tru9I TTAA 3 cut(s) 516, 638, 1209
TscAI CASTG 9 cut(s) 29, 232, 595, 725, 969, 1168, 1250, 1428, 1480
TseFI GTSAC 3 cut(s) 145, 347, 1696
TseI GCWGC 2 cut(s) 1598, 1685
Tsp45I GTSAC 3 cut(s) 145, 347, 1696
TspDTI ATGAA 6 cut(s) 113, 167, 206, 695, 1062, 1227
TspGWI ACGGA 5 cut(s) 255, 662, 679, 920, 1697
TspRI CASTG 9 cut(s) 29, 232, 595, 725, 969, 1168, 1250, 1428, 1480
VpaK11BI GGWCC 7 cut(s) 4, 169, 308, 359, 476, 1151, 1166
XapI RAATTY 3 cut(s) 295, 481, 1437
XceI RCATGY 2 cut(s) 1189, 1502
XmnI GAANNNNTTC 1 cut(s) 1029
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.