Rroxscaffold_3G00244570

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001995
Physical Location & Seq
Forward (+)
37415851 .. 37418778
2928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00244570.1

Sequence Viewer

Length: 2532 bp
ATGGTGAAGTTTACAGCTGAGGAGCTTCGCCGGATAATGGACTACAAACACAACATTCGCAATATGTCTGTTATTGCACATGTTGATCACGGGAAGTCAACCCTTACGGATTCTCTTGTGGCTGCTGCTGGTATCATTGCCCAAGAGGTTGCTGGTGATGTTCGGATGACAGATACCCGTGCTGATGAGGCAGAGCGTGGCATCACAATCAAATCTACTGGTATCTCCCTCTATTATGAAATGTCTGATGATGCTCTGAAGAGCTACAAGGGAGAAAGAAACGGAAATGAGTACCTCATCAACCTCATTGATTCCCCTGGGCACGTTGACTTTTCATCTGAGGTCACAGCTGCTCTTCGCATTACTGATGGTGCACTTGTGGTCGTTGATTGCATTGAGGGTGTTTGTGTACAAACAGAGACTGTGCTTCGTCAAGCCTTGGGAGAAAGGATCAGGCCTGTTTTGACTGTTAACAAGATGGACAGGTGCTTCCTTGAGCTCCAGGTGGATGGAGAGGAGGCGTACCAAGCATTTCAAAGGGTTATTGAGAATGCTAATGTCATTATGGCTACCTATGAAGACCCCCTCCTTGGTGATGTCCAGGTCTACCCTGAGAAAGGAACGGTTGCTTTCTCTGCTGGTTTGCATGGTTGGGCTTTCACTCTAACCAACTTTGCCAAGATGTATGCATCCAAGTTTGGAGTTGATGAGTCAAAGATGATGGAAAGGCTCTGGGGTGAGAACTATTTTGACCCAGCTACCAAGAAATGGACCACCAAGAGTACTGGCTCTGCTACCTGCAAGCGTGGTTTCGTTCAGTTCTGCTATGAACCCATCAAGCAGATTATCAACACTTGCATGAATGACGAGAAGGACAAGCTGTGGCCTATGTTGACAAAGCTTGGAGTCACCATGAAGGGTGACGAAAAGGAGCTGATGGGAAAAGCACTTATGAAGCGTGTTATGCAGAACTGGCTGCCAGCCAGCAGTGCCTTGTTGGAAATGATGATCTTTCATCTTCCCTCTCCTTCAACTGCTCAGAGGTATCGTGTGGAGAATTTGTATGAGGGTCCCCTTGATGACCAATATGCCAATGCCATTAGGAACTGTGATCCTGAAGGACCTCTTATGCTGTATGTGTCTAAGATGATTCCTGCCTCTGACAAGGGTAGATTCTTTGCTTTTGGTCGTGTGTTTGCTGGGAAAGTCCATACAGGTGTGAAGGTTAGAATTATGGGTCCAAATTACGTTCCTGGAGAAAAGAAGGATTTGTATGTTAAGAATGTACAGAGAACTGTAATCTGGATGGGAAAGAAACAAGAAACTGTTGAGGACGTACCTTGTGGTAACACTGTTGCCTTGGTTGGTCTGGATCAGTTTATCACCAAGAATGCTACATTAACAAATGAAAAGGAAACCGATGCCCACCCCATTCGTGCTATGAAGTTCTCTGTCTCACCCGTTGTGCGTGTTGCTGTTCAATGCAAGGTTGCTTCTGACCTTCCCAAACTTGTTGAAGGTCTCAAACGTCTGGCAAAGTCTGATCCTATGGTTGTCTGTTCCATTGAGGAGTCTGGGGAGCACATTATTGCAGGTGCTGGAGAACTTCACCTTGAAATCTGTCTGAAGGATCTTCAGGATGACTTTATGGGTGGAGCAGAAATTATAAAATCTGATCCTGTTGTGTCCTTCCGTGAGACGGTCCTCGAGAAGTCTAGCCGTACAGTGATGAGCAAGTCACCCAACAAGCATAACCGCCTGTACATGGAAGCACGGCCATTGGAGGAAGGTCTTCCTGAGGCCATTGATGATGGTCGTATTGGTCCAAGGGATGATCCCAAAATTCGTTCTAAAATCTTGGCTGAGGAATTTGGTTGGGACAAGGATCTTGCTAAGAAAATCTGGTGTTTTGGCCCTGAGACTATTGGTCCTAACATGGTTGTTGACATGTGTAAGGGAGTCCAGTACCTCAATGAAATCAAGGACTCTGTCGTTGCTGGGTTCCAGTGGGCTTCAAAGGAAGGGGCATTGGCTGAAGAAAACATGAGGGGTATTTGCTTTGAAATCTGTGATGTGGTTCTTCATGCTGATGCTATTCATAGAGGAGGTGGTCAGGTTATTCCAACTGCTAGGAGGGTCATCTATGCTTCTCAGCTTACAGCTAAGCCAAGGCTCCTTGAACCTGTGTATCTTGTTGAAATTCAAGCTCCTGAGAATGCTCTTGGTGGTATCTACAGCGTTCTTAATCAGAAGCGTGGACATGTGTTTGAAGAAATGCAAAGGCCTGGTACTCCTCTGTACAACATCAAGGCATACCTGCCTGTCATTGAATCTTTTGGGTTCTCTGGTCAGTTGAGGGCTGCCACTTCAGGACAGGCTTTCCCGCAGTGCGTGTTTGATCATTGGGACATGATGTCTTCTGATCCATTGGAAGCTGGTTCACAGGCTGCACAGCTTGTTACAGACATCCGTAGGAGGAAGGGTTTGAAGGAGCAGATGACTCCACTTTCTGAGTTCGAGGACAAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04152 ko04921 map04152 map04921
Pfam Domains
Protein Families
EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

843

Amino Acids

94.02

Weight (kDa)

5.78

Isoelectric Point (pI)

36.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 18 - 221 1.9e-48 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 379 - 469 1.8e-06 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 394 - 462 6.3e-10 Elongation factor Tu domain 2
EFG_III PF14492 487 - 549 1e-11 Elongation Factor G, domain III
EFG_IV PF03764 610 - 722 4.4e-29 Elongation factor G, domain IV
EFG_C PF00679 724 - 811 7.4e-21 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1667
AarI CACCTGC 1 cut(s) 1583
Acc36I ACCTGC 3 cut(s) 806, 1583, 2325
AccB7I CCANNNNNTGG 1 cut(s) 768
AccI GTMKAC 1 cut(s) 606
AciI CCGC 2 cut(s) 1756, 2384
AclWI GGATC 9 cut(s) 458, 1106, 1380, 1538, 1638, 1670, 1829, 1893, 2417
AcoI YGGCCR 1 cut(s) 1775
AcsI RAATTY 4 cut(s) 1057, 1842, 1868, 2199
AcuI CTGAAG 6 cut(s) 278, 1137, 1619, 1646, 2055, 2352
AfiI CCNNNNNNNGG 6 cut(s) 37, 590, 617, 768, 1699, 1765
AflIII ACRYGT 3 cut(s) 79, 1947, 2260
AhdI GACNNNNNGTC 2 cut(s) 1926, 2413
AjnI CCWGG 5 cut(s) 316, 501, 600, 1252, 2284
Alw21I GWGCWC 3 cut(s) 376, 501, 1584
Alw26I GTCTC 5 cut(s) 413, 1459, 1526, 1691, 1913
Alw44I GTGCAC 1 cut(s) 372
AlwI GGATC 9 cut(s) 458, 1106, 1380, 1538, 1638, 1670, 1829, 1893, 2417
AlwNI CAGNNNCTG 2 cut(s) 422, 1598
Ama87I CYCGRG 1 cut(s) 1706
AoxI GGCC 6 cut(s) 455, 884, 1775, 1800, 1912, 2282
ApaLI GTGCAC 1 cut(s) 372
ApeKI GCWGC 6 cut(s) 122, 125, 350, 976, 2360, 2447
ApoI RAATTY 4 cut(s) 1057, 1842, 1868, 2199
Asp700I GAANNNNTTC 1 cut(s) 1791
AspS9I GGNCC 8 cut(s) 771, 1070, 1121, 1238, 1702, 1823, 1913, 1928
AvaI CYCGRG 1 cut(s) 1706
AvaII GGWCC 7 cut(s) 771, 1070, 1121, 1238, 1702, 1823, 1928
AxyI CCTNAGG 1 cut(s) 1797
BaeGI GKGCMC 2 cut(s) 324, 376
BaeI ACNNNNGTAYC 2 cut(s) 2171, 2204
BanII GRGCYC 1 cut(s) 501
BbsI GAAGAC 3 cut(s) 585, 1784, 2409
Bbv12I GWGCWC 3 cut(s) 376, 501, 1584
BbvCI CCTCAGC 2 cut(s) 18, 1863
BbvI GCAGC 6 cut(s) 109, 112, 337, 963, 2347, 2434
BccI CCATC 8 cut(s) 362, 472, 503, 715, 842, 931, 1300, 1805
BceAI ACGGC 2 cut(s) 1704, 1790
BciT130I CCWGG 5 cut(s) 318, 503, 602, 1254, 2286
BclI TGATCA 2 cut(s) 85, 2398
BcoDI GTCTC 5 cut(s) 413, 1459, 1526, 1691, 1913
BfaI CTAG 2 cut(s) 1716, 2130
BfmI CTRYAG 1 cut(s) 2233
BfuAI ACCTGC 3 cut(s) 806, 1583, 2325
BisI GCNGC 6 cut(s) 123, 126, 351, 977, 2361, 2448
BlpI GCTNAGC 1 cut(s) 2163
BlsI GCNGC 6 cut(s) 124, 127, 352, 978, 2362, 2449
BmcAI AGTACT 1 cut(s) 784
Bme1390I CCNGG 5 cut(s) 318, 503, 602, 1254, 2286
Bme18I GGWCC 7 cut(s) 771, 1070, 1121, 1238, 1702, 1823, 1928
BmeRI GACNNNNNGTC 2 cut(s) 1926, 2413
BmeT110I CYCGRG 1 cut(s) 1706
BmgT120I GGNCC 8 cut(s) 771, 1070, 1121, 1238, 1702, 1823, 1913, 1928
BmiI GGNNCC 5 cut(s) 1071, 1072, 1239, 2003, 2174
BmrFI CCNGG 5 cut(s) 318, 503, 602, 1254, 2286
BmsI GCATC 5 cut(s) 210, 241, 698, 1411, 2080
BpiI GAAGAC 3 cut(s) 585, 1784, 2409
BpmI CTGGAG 3 cut(s) 485, 1275, 1620
Bpu10I CCTNAGC 2 cut(s) 18, 1863
Bpu1102I GCTNAGC 1 cut(s) 2163
BpuEI CTTGAG 1 cut(s) 515
BsaI GGTCTC 1 cut(s) 1526
BsaJI CCNNGG 7 cut(s) 316, 317, 438, 589, 1359, 1826, 2168
BsaXI ACNNNNNCTCC 4 cut(s) 1046, 1076, 2467, 2497
Bsc4I CCNNNNNNNGG 6 cut(s) 37, 590, 617, 768, 1699, 1765
Bse1I ACTGG 5 cut(s) 223, 790, 977, 1963, 2005
Bse21I CCTNAGG 1 cut(s) 1797
Bse3DI GCAATG 1 cut(s) 135
BseBI CCWGG 5 cut(s) 318, 503, 602, 1254, 2286
BseDI CCNNGG 7 cut(s) 316, 317, 438, 589, 1359, 1826, 2168
BseGI GGATG 7 cut(s) 171, 514, 689, 1311, 1645, 1837, 2466
BseLI CCNNNNNNNGG 6 cut(s) 37, 590, 617, 768, 1699, 1765
BseMI GCAATG 1 cut(s) 135
BseNI ACTGG 5 cut(s) 223, 790, 977, 1963, 2005
BseRI GAGGAG 5 cut(s) 35, 530, 1583, 2118, 2283
BseSI GKGCMC 2 cut(s) 324, 376
BseXI GCAGC 6 cut(s) 109, 112, 337, 963, 2347, 2434
BseYI CCCAGC 3 cut(s) 754, 1199, 1997
BsgI GTGCAG 1 cut(s) 2433
BshFI GGCC 6 cut(s) 457, 886, 1777, 1802, 1914, 2284
BsiHKAI GWGCWC 3 cut(s) 376, 501, 1584
BsiHKCI CYCGRG 1 cut(s) 1706
BsiSI CCGG 1 cut(s) 31
BslFI GGGAC 3 cut(s) 1056, 1892, 2420
BslI CCNNNNNNNGG 6 cut(s) 37, 590, 617, 768, 1699, 1765
BsmAI GTCTC 5 cut(s) 413, 1459, 1526, 1691, 1913
BsmBI CGTCTC 1 cut(s) 1691
BsmFI GGGAC 3 cut(s) 1056, 1892, 2420
BsmI GAATGC 3 cut(s) 556, 1396, 2221
BsnI GGCC 6 cut(s) 457, 886, 1777, 1802, 1914, 2284
Bso31I GGTCTC 1 cut(s) 1526
BsoBI CYCGRG 1 cut(s) 1706
Bsp1286I GDGCHC 4 cut(s) 324, 376, 501, 1584
Bsp1407I TGTACA 4 cut(s) 409, 1285, 1761, 2298
Bsp1720I GCTNAGC 1 cut(s) 2163
BspACI CCGC 2 cut(s) 1756, 2384
BspANI GGCC 6 cut(s) 457, 886, 1777, 1802, 1914, 2284
BspLI GGNNCC 5 cut(s) 1071, 1072, 1239, 2003, 2174
BspMI ACCTGC 3 cut(s) 806, 1583, 2325
BspPI GGATC 9 cut(s) 458, 1106, 1380, 1538, 1638, 1670, 1829, 1893, 2417
BspQI GCTCTTC 2 cut(s) 254, 360
BspTNI GGTCTC 1 cut(s) 1526
BsrDI GCAATG 1 cut(s) 135
BsrGI TGTACA 4 cut(s) 409, 1285, 1761, 2298
BsrI ACTGG 5 cut(s) 223, 790, 977, 1963, 2005
BssECI CCNNGG 7 cut(s) 316, 317, 438, 589, 1359, 1826, 2168
BssT1I CCWWGG 5 cut(s) 438, 589, 1359, 1826, 2168
Bst2UI CCWGG 5 cut(s) 318, 503, 602, 1254, 2286
Bst4CI ACNGT 9 cut(s) 424, 469, 625, 1109, 1297, 1327, 1354, 1702, 1726
Bst6I CTCTTC 2 cut(s) 254, 360
BstAUI TGTACA 4 cut(s) 409, 1285, 1761, 2298
BstC8I GCNNGC 3 cut(s) 803, 981, 985
BstENI CCTNNNNNAGG 1 cut(s) 615
BstF5I GGATG 7 cut(s) 171, 514, 689, 1311, 1645, 1837, 2466
BstMAI GTCTC 5 cut(s) 413, 1459, 1526, 1691, 1913
BstMWI GCNNNNNNNGC 6 cut(s) 188, 527, 635, 964, 973, 989
BstNI CCWGG 5 cut(s) 318, 503, 602, 1254, 2286
BstNSI RCATGY 3 cut(s) 83, 1951, 2264
BstSCI CCNGG 5 cut(s) 316, 501, 600, 1252, 2284
BstSFI CTRYAG 1 cut(s) 2233
BstSLI GKGCMC 2 cut(s) 324, 376
BstV1I GCAGC 6 cut(s) 109, 112, 337, 963, 2347, 2434
BstV2I GAAGAC 3 cut(s) 585, 1784, 2409
BstX2I RGATCY 2 cut(s) 1630, 1885
BstXI CCANNNNNNTGG 1 cut(s) 509
BstYI RGATCY 2 cut(s) 1630, 1885
Bsu36I CCTNAGG 1 cut(s) 1797
BsuRI GGCC 6 cut(s) 457, 886, 1777, 1802, 1914, 2284
BtsCI GGATG 7 cut(s) 171, 514, 689, 1311, 1645, 1837, 2466
BtsI GCAGTG 2 cut(s) 994, 2393
BtsIMutI CAGTG 5 cut(s) 994, 1350, 1731, 2012, 2393
BveI ACCTGC 3 cut(s) 806, 1583, 2325
Cac8I GCNNGC 3 cut(s) 803, 981, 985
CaiI CAGNNNCTG 2 cut(s) 422, 1598
Cfr13I GGNCC 8 cut(s) 771, 1070, 1121, 1238, 1702, 1823, 1913, 1928
DriI GACNNNNNGTC 2 cut(s) 1926, 2413
EaeI YGGCCR 1 cut(s) 1775
Eam1104I CTCTTC 2 cut(s) 254, 360
Eam1105I GACNNNNNGTC 2 cut(s) 1926, 2413
EarI CTCTTC 2 cut(s) 254, 360
Ecl136II GAGCTC 1 cut(s) 499
Eco130I CCWWGG 5 cut(s) 438, 589, 1359, 1826, 2168
Eco147I AGGCCT 2 cut(s) 457, 2284
Eco24I GRGCYC 1 cut(s) 501
Eco31I GGTCTC 1 cut(s) 1526
Eco47I GGWCC 7 cut(s) 771, 1070, 1121, 1238, 1702, 1823, 1928
Eco53kI GAGCTC 1 cut(s) 499
Eco57I CTGAAG 6 cut(s) 278, 1137, 1619, 1646, 2055, 2352
Eco81I CCTNAGG 1 cut(s) 1797
Eco88I CYCGRG 1 cut(s) 1706
EcoICRI GAGCTC 1 cut(s) 499
EcoNI CCTNNNNNAGG 1 cut(s) 615
EcoO109I RGGNCCY 2 cut(s) 1070, 1121
EcoRII CCWGG 5 cut(s) 316, 501, 600, 1252, 2284
EcoT14I CCWWGG 5 cut(s) 438, 589, 1359, 1826, 2168
EcoT22I ATGCAT 1 cut(s) 691
EcoT38I GRGCYC 1 cut(s) 501
ErhI CCWWGG 5 cut(s) 438, 589, 1359, 1826, 2168
Esp3I CGTCTC 1 cut(s) 1691
FalI AAGNNNNNCTT 2 cut(s) 1110, 1142
FaqI GGGAC 3 cut(s) 1056, 1892, 2420
FauI CCCGC 1 cut(s) 2391
FbaI TGATCA 2 cut(s) 85, 2398
FblI GTMKAC 1 cut(s) 606
Fnu4HI GCNGC 6 cut(s) 123, 126, 351, 977, 2361, 2448
FokI GGATG 7 cut(s) 178, 521, 676, 1318, 1652, 1844, 2453
FriOI GRGCYC 1 cut(s) 501
Fsp4HI GCNGC 6 cut(s) 123, 126, 351, 977, 2361, 2448
FspBI CTAG 2 cut(s) 1716, 2130
GluI GCNGC 6 cut(s) 123, 126, 351, 977, 2361, 2448
GsaI CCCAGC 3 cut(s) 758, 1203, 2001
GsuI CTGGAG 3 cut(s) 485, 1275, 1620
HaeIII GGCC 6 cut(s) 457, 886, 1777, 1802, 1914, 2284
HapII CCGG 1 cut(s) 31
HincII GTYRAC 5 cut(s) 99, 328, 472, 894, 1945
HindII GTYRAC 5 cut(s) 99, 328, 472, 894, 1945
HindIII AAGCTT 2 cut(s) 899, 2525
HpaI GTTAAC 1 cut(s) 472
HpaII CCGG 1 cut(s) 31
Hpy188III TCNNGA 8 cut(s) 1115, 1303, 1370, 1637, 1708, 1796, 2210, 2370
HpyCH4III ACNGT 9 cut(s) 424, 469, 625, 1109, 1297, 1327, 1354, 1702, 1726
HpyCH4IV ACGT 4 cut(s) 324, 1248, 1335, 1528
HpyF10VI GCNNNNNNNGC 6 cut(s) 188, 527, 635, 964, 973, 989
HpySE526I ACGT 4 cut(s) 324, 1248, 1335, 1528
KflI GGGWCCC 1 cut(s) 1070
Ksp22I TGATCA 2 cut(s) 85, 2398
KspAI GTTAAC 1 cut(s) 472
LguI GCTCTTC 2 cut(s) 254, 360
LmnI GCTCC 8 cut(s) 22, 504, 931, 1579, 1655, 2178, 2212, 2491
Lsp1109I GCAGC 6 cut(s) 109, 112, 337, 963, 2347, 2434
LweI GCATC 5 cut(s) 210, 241, 698, 1411, 2080
MaeI CTAG 2 cut(s) 1716, 2130
MaeII ACGT 4 cut(s) 324, 1248, 1335, 1528
MaeIII GTNAC 6 cut(s) 343, 907, 920, 1346, 1737, 2458
MflI RGATCY 2 cut(s) 1630, 1885
MhlI GDGCHC 4 cut(s) 324, 376, 501, 1584
MluCI AATT 7 cut(s) 1057, 1230, 1243, 1662, 1842, 1868, 2199
MlyI GAGTC 6 cut(s) 719, 915, 1580, 1968, 1979, 2494
MmeI TCCRAC 2 cut(s) 978, 2147
Mph1103I ATGCAT 1 cut(s) 691
MroXI GAANNNNTTC 1 cut(s) 1791
MseI TTAA 5 cut(s) 471, 1278, 1400, 2244, 2530
MslI CAYNNNNRTG 3 cut(s) 857, 1215, 2088
MspA1I CMGCKG 2 cut(s) 17, 350
MspI CCGG 1 cut(s) 31
MspR9I CCNGG 5 cut(s) 318, 503, 602, 1254, 2286
Mva1269I GAATGC 3 cut(s) 556, 1396, 2221
MvaI CCWGG 5 cut(s) 318, 503, 602, 1254, 2286
MwoI GCNNNNNNNGC 6 cut(s) 188, 527, 635, 964, 973, 989
NlaIV GGNNCC 5 cut(s) 1071, 1072, 1239, 2003, 2174
NmuCI GTSAC 4 cut(s) 343, 907, 920, 1737
NsiI ATGCAT 1 cut(s) 691
NspI RCATGY 3 cut(s) 83, 1951, 2264
PaeR7I CTCGAG 1 cut(s) 1706
PaqCI CACCTGC 1 cut(s) 1583
PasI CCCWGGG 1 cut(s) 317
PceI AGGCCT 2 cut(s) 457, 2284
PciI ACATGT 3 cut(s) 79, 1947, 2260
PciSI GCTCTTC 2 cut(s) 254, 360
PctI GAATGC 3 cut(s) 556, 1396, 2221
PdmI GAANNNNTTC 1 cut(s) 1791
PfeI GAWTC 5 cut(s) 110, 311, 1150, 1173, 2330
PflFI GACNNNGTC 1 cut(s) 1988
PflMI CCANNNNNTGG 1 cut(s) 768
PfoI TCCNGGA 1 cut(s) 1252
PkrI GCNGC 6 cut(s) 124, 127, 352, 978, 2362, 2449
PleI GAGTC 6 cut(s) 718, 914, 1579, 1967, 1979, 2494
PpsI GAGTC 6 cut(s) 718, 914, 1579, 1967, 1979, 2494
PpuMI RGGWCCY 2 cut(s) 1070, 1121
PscI ACATGT 3 cut(s) 79, 1947, 2260
PsiI TTATAA 1 cut(s) 1667
Psp124BI GAGCTC 1 cut(s) 501
Psp5II RGGWCCY 2 cut(s) 1070, 1121
Psp6I CCWGG 5 cut(s) 316, 501, 600, 1252, 2284
PspFI CCCAGC 3 cut(s) 754, 1199, 1997
PspGI CCWGG 5 cut(s) 316, 501, 600, 1252, 2284
PspN4I GGNNCC 5 cut(s) 1071, 1072, 1239, 2003, 2174
PspPI GGNCC 8 cut(s) 771, 1070, 1121, 1238, 1702, 1823, 1913, 1928
PspPPI RGGWCCY 2 cut(s) 1070, 1121
PstNI CAGNNNCTG 2 cut(s) 422, 1598
PsuI RGATCY 2 cut(s) 1630, 1885
PsyI GACNNNGTC 1 cut(s) 1988
PvuII CAGCTG 2 cut(s) 17, 350
RseI CAYNNNNRTG 3 cut(s) 857, 1215, 2088
SacI GAGCTC 1 cut(s) 501
SapI GCTCTTC 2 cut(s) 254, 360
SaqAI TTAA 5 cut(s) 471, 1278, 1400, 2244, 2530
SatI GCNGC 6 cut(s) 123, 126, 351, 977, 2361, 2448
Sau96I GGNCC 8 cut(s) 771, 1070, 1121, 1238, 1702, 1823, 1913, 1928
ScaI AGTACT 1 cut(s) 784
SchI GAGTC 6 cut(s) 719, 915, 1580, 1968, 1979, 2494
ScrFI CCNGG 5 cut(s) 318, 503, 602, 1254, 2286
SduI GDGCHC 4 cut(s) 324, 376, 501, 1584
SfaNI GCATC 5 cut(s) 210, 241, 698, 1411, 2080
SfcI CTRYAG 1 cut(s) 2233
Sfr274I CTCGAG 1 cut(s) 1706
SinI GGWCC 7 cut(s) 771, 1070, 1121, 1238, 1702, 1823, 1928
SlaI CTCGAG 1 cut(s) 1706
SmiMI CAYNNNNRTG 3 cut(s) 857, 1215, 2088
SmlI CTYRAG 2 cut(s) 494, 1706
SmoI CTYRAG 2 cut(s) 494, 1706
Sse9I AATT 7 cut(s) 1057, 1230, 1243, 1662, 1842, 1868, 2199
SseBI AGGCCT 2 cut(s) 457, 2284
SsiI CCGC 2 cut(s) 1756, 2384
SspMI CTAG 2 cut(s) 1716, 2130
SstI GAGCTC 1 cut(s) 501
StuI AGGCCT 2 cut(s) 457, 2284
StyD4I CCNGG 5 cut(s) 316, 501, 600, 1252, 2284
StyI CCWWGG 5 cut(s) 438, 589, 1359, 1826, 2168
TaaI ACNGT 9 cut(s) 424, 469, 625, 1109, 1297, 1327, 1354, 1702, 1726
TaiI ACGT 4 cut(s) 327, 1251, 1338, 1531
TaqI TCGA 2 cut(s) 1707, 2517
TasI AATT 7 cut(s) 1057, 1230, 1243, 1662, 1842, 1868, 2199
TatI WGTACW 5 cut(s) 409, 782, 1285, 1761, 2298
TfiI GAWTC 5 cut(s) 110, 311, 1150, 1173, 2330
Tru1I TTAA 5 cut(s) 471, 1278, 1400, 2244, 2530
Tru9I TTAA 5 cut(s) 471, 1278, 1400, 2244, 2530
TscAI CASTG 5 cut(s) 994, 1357, 1731, 2012, 2393
TseFI GTSAC 4 cut(s) 343, 907, 920, 1737
TseI GCWGC 6 cut(s) 122, 125, 350, 976, 2360, 2447
Tsp45I GTSAC 4 cut(s) 343, 907, 920, 1737
TspGWI ACGGA 4 cut(s) 122, 297, 1682, 2459
TspRI CASTG 5 cut(s) 994, 1357, 1731, 2012, 2393
Tth111I GACNNNGTC 1 cut(s) 1988
Van91I CCANNNNNTGG 1 cut(s) 768
VneI GTGCAC 1 cut(s) 372
VpaK11BI GGWCC 7 cut(s) 771, 1070, 1121, 1238, 1702, 1823, 1928
XagI CCTNNNNNAGG 1 cut(s) 615
XapI RAATTY 4 cut(s) 1057, 1842, 1868, 2199
XceI RCATGY 3 cut(s) 83, 1951, 2264
XcmI CCANNNNNNNNNTGG 1 cut(s) 149
XhoI CTCGAG 1 cut(s) 1706
XmiI GTMKAC 1 cut(s) 606
XmnI GAANNNNTTC 1 cut(s) 1791
XspI CTAG 2 cut(s) 1716, 2130
ZrmI AGTACT 1 cut(s) 784
Zsp2I ATGCAT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.