pycom05g20420

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0001995
Physical Location & Seq
Reverse (-)
23183916 .. 23185319
1404 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g20420.1

Sequence Viewer

Length: 1404 bp
ATGCTTTATGTATCTAAGATGATTCCTGCATCTGACAAGGGCAGATTCTTTGCCTTTGGTCGTGTCTTCTCTGGCAGAGTGTCTACTGGTTTGAAGGTCAGAATCATGGGTCCAAATTTTGTTCCTGGAGAGAAGAAGGATTTGTATGTGAAGAATGTACAGAGGACCGTCATTTGGATGGGCAAGAAACAAGAAACGGTCGAGGATGTGCCTTGTGGCAACACTGTTGCCCTGGTTGGTCTGGATCAATTTATCACCAAGAATGCTACCTTGACGAATGAGAAGGAAGTGGATGCTCACCCTATTCGTGCTATGAAGTTCTCTGTTTCGCCCGTTGTGCGTGTTGCTGTTCAGTGCAAAGTCGCGTCTGACCTGCCAAAACTTGTTGAAGGTCTCAAGCGTCTGTCCAAGTCCGATCCTATGGTTGTCTGTACCATTGAGGAGTCGGGCGAGCACATAATTGCCGGTGCTGGTGAACTTCACCTCGAAATCTGTTTGAAGGATCTGCAAGAAGACTTCATGGGCGGAGCTGAGATTATAAAGTCTGATCCTGTCGTCTCTTTCCGTGAGACTGTCCTTGAGAAGTCCAGCCGTACAGTTATGAGCAAGTCTCCCAACAAGCACAACCGTCTCTACATGGAAGCACGCCCCTTGGAGGAAGGTCTTCCCGAGGCTATTGATGATGGCCGTATTGGGCCAAGAGATGATCTCAAGATTCGTTCCAAGATCTTGGCGGAGGAGTTCGGTTGGGACAAGGATCTTGCAAAGAAAATCTGGTGTTTTGGTCCCGAGACCACTGGTCCTAACATGGTGGTCGACATGTGTAAGGGAGTTCAGTACCTTAATGAAATCAAGGATTCCGTTGTCGCTGGGTTCCAGTGGGCCTCAAAGGAAGGTGCATTGGCAGAAGAAAACATGAGAGGTATTTGCTTTGAAGTCTGCGATGTGGTTCTCCATGCTGATGCCATCCACAGGGGTGGTGGCCAGGTCATTCCAACTGCTCGAAGGGTCATCTACGCGTCCCAGCTCACAGCCAAGCCAAGGCTCCTTGAACCAGTCTATCTTGTCGAAATCCAAACTCCGGAGCAAGCTCTTGGCGGCATCTACAGCGTTCTTAATCAGAAGCGAGGGCACGTCTTTGAGGAAATTCAGAGGCCTGGCACTCCGCTCTACAACATCAAGGCTTACCTCCCCGTCATTGAATCTTTTGGATTCTCTAGTCAGTTAAGAGCTTCTACTTCCGGTCAGGCTTTCCCACAATGCGTGTTTGATCACTGGGAGATGATGTCGTCTGATCCACTGGAACCAGGAACGCAGGCTTCAACACTCGTTGCCGACATCCGCAAAAGGAAGGGTTTGAAGGAGCAGATGACCCCGCTCTCCGAGTACGAGGATAAGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04152 ko04921 map04152 map04921
Pfam Domains
Protein Families
EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

468

Amino Acids

51.92

Weight (kDa)

6.5

Isoelectric Point (pI)

38.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-G_D2 PF22042 12 - 93 6.5e-07 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 18 - 86 6.7e-09 Elongation factor Tu domain 2
EFG_III PF14492 111 - 173 2.2e-12 Elongation Factor G, domain III
EFG_IV PF03764 235 - 346 1.5e-29 Elongation factor G, domain IV
EFG_C PF00679 348 - 434 1.2e-21 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 539
Acc36I ACCTGC 1 cut(s) 381
AccBSI CCGCTC 2 cut(s) 1168, 1378
AccI GTMKAC 2 cut(s) 83, 816
AccII CGCG 2 cut(s) 365, 1019
AccIII TCCGGA 1 cut(s) 1081
AciI CCGC 6 cut(s) 525, 734, 1098, 1166, 1342, 1376
AclWI GGATC 6 cut(s) 252, 410, 510, 542, 765, 1289
AcoI YGGCCR 2 cut(s) 685, 982
AcsI RAATTY 2 cut(s) 115, 1146
AfaI GTAC 5 cut(s) 159, 433, 595, 839, 1388
AfiI CCNNNNNNNGG 5 cut(s) 174, 655, 972, 1041, 1081
AflIII ACRYGT 2 cut(s) 819, 1017
AgsI TTSAA 8 cut(s) 94, 389, 499, 935, 1052, 1202, 1323, 1360
AhdI GACNNNNNGTC 1 cut(s) 798
AjiI CACGTC 1 cut(s) 1135
AjnI CCWGG 5 cut(s) 124, 231, 984, 1156, 1306
AleI CACNNNNGTG 1 cut(s) 975
AluBI AGCT 5 cut(s) 530, 1027, 1091, 1232, 1399
AluI AGCT 5 cut(s) 530, 1027, 1091, 1232, 1399
Alw21I GWGCWC 1 cut(s) 456
Alw26I GTCTC 6 cut(s) 398, 562, 563, 615, 635, 785
AlwI GGATC 6 cut(s) 252, 410, 510, 542, 765, 1289
Ama87I CYCGRG 2 cut(s) 668, 788
Aor13HI TCCGGA 1 cut(s) 1081
AoxI GGCC 5 cut(s) 685, 695, 882, 982, 1154
ApoI RAATTY 2 cut(s) 115, 1146
Asp700I GAANNNNTTC 1 cut(s) 663
AspS9I GGNCC 6 cut(s) 110, 165, 695, 785, 800, 882
AsuHPI GGTGA 4 cut(s) 247, 290, 473, 485
AvaI CYCGRG 2 cut(s) 668, 788
AvaII GGWCC 4 cut(s) 110, 165, 785, 800
BaeGI GKGCMC 1 cut(s) 1134
BalI TGGCCA 1 cut(s) 984
BbsI GAAGAC 3 cut(s) 58, 519, 656
Bbv12I GWGCWC 1 cut(s) 456
BccI CCATC 3 cut(s) 172, 677, 974
BceAI ACGGC 2 cut(s) 576, 672
BciT130I CCWGG 5 cut(s) 126, 233, 986, 1158, 1308
BclI TGATCA 1 cut(s) 1270
BcoDI GTCTC 6 cut(s) 398, 562, 563, 615, 635, 785
BfaI CTAG 1 cut(s) 1218
BfmI CTRYAG 1 cut(s) 1105
BfuAI ACCTGC 1 cut(s) 381
BglII AGATCT 1 cut(s) 726
BisI GCNGC 1 cut(s) 1099
BlsI GCNGC 1 cut(s) 1100
Bme1390I CCNGG 5 cut(s) 126, 233, 986, 1158, 1308
Bme18I GGWCC 4 cut(s) 110, 165, 785, 800
BmeRI GACNNNNNGTC 1 cut(s) 798
BmeT110I CYCGRG 2 cut(s) 668, 788
BmgBI CACGTC 1 cut(s) 1135
BmgT120I GGNCC 6 cut(s) 110, 165, 695, 785, 800, 882
BmiI GGNNCC 5 cut(s) 111, 787, 875, 1046, 1305
BmrFI CCNGG 5 cut(s) 126, 233, 986, 1158, 1308
BmrI ACTGGG 1 cut(s) 1285
BmsI GCATC 4 cut(s) 38, 283, 952, 1110
BmuI ACTGGG 1 cut(s) 1285
BpiI GAAGAC 3 cut(s) 58, 519, 656
BplI GAGNNNNNCTC 4 cut(s) 595, 627, 693, 725
BpmI CTGGAG 1 cut(s) 147
BpuEI CTTGAG 3 cut(s) 380, 599, 695
BsaI GGTCTC 2 cut(s) 398, 785
BsaJI CCNNGG 4 cut(s) 231, 651, 669, 1040
BsaWI WCCGGW 2 cut(s) 1081, 1241
BsaXI ACNNNNNCTCC 2 cut(s) 1271, 1301
Bsc4I CCNNNNNNNGG 5 cut(s) 174, 655, 972, 1041, 1081
Bse118I RCCGGY 1 cut(s) 464
Bse1I ACTGG 6 cut(s) 91, 802, 877, 1055, 1280, 1305
BseAI TCCGGA 1 cut(s) 1081
BseBI CCWGG 5 cut(s) 126, 233, 986, 1158, 1308
BseDI CCNNGG 4 cut(s) 231, 651, 669, 1040
BseGI GGATG 5 cut(s) 183, 211, 298, 966, 1338
BseLI CCNNNNNNNGG 5 cut(s) 174, 655, 972, 1041, 1081
BseMII CTCAG 1 cut(s) 522
BseNI ACTGG 6 cut(s) 91, 802, 877, 1055, 1280, 1305
BseRI GAGGAG 2 cut(s) 455, 752
BseSI GKGCMC 1 cut(s) 1134
BseYI CCCAGC 2 cut(s) 869, 1023
Bsh1236I CGCG 2 cut(s) 365, 1019
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 5 cut(s) 687, 697, 884, 984, 1156
BsiEI CGRYCG 1 cut(s) 201
BsiHKAI GWGCWC 1 cut(s) 456
BsiHKCI CYCGRG 2 cut(s) 668, 788
BsiSI CCGG 3 cut(s) 465, 1082, 1242
BslFI GGGAC 3 cut(s) 764, 771, 1006
BslI CCNNNNNNNGG 5 cut(s) 174, 655, 972, 1041, 1081
BsmAI GTCTC 6 cut(s) 398, 562, 563, 615, 635, 785
BsmBI CGTCTC 2 cut(s) 562, 635
BsmFI GGGAC 3 cut(s) 764, 771, 1006
BsmI GAATGC 1 cut(s) 268
BsnI GGCC 5 cut(s) 687, 697, 884, 984, 1156
Bso31I GGTCTC 2 cut(s) 398, 785
BsoBI CYCGRG 2 cut(s) 668, 788
Bsp1286I GDGCHC 2 cut(s) 456, 1134
Bsp13I TCCGGA 1 cut(s) 1081
Bsp1407I TGTACA 1 cut(s) 157
Bsp143I GATC 9 cut(s) 244, 415, 502, 547, 706, 726, 757, 1270, 1294
BspACI CCGC 6 cut(s) 525, 734, 1098, 1166, 1342, 1376
BspANI GGCC 5 cut(s) 687, 697, 884, 984, 1156
BspCNI CTCAG 1 cut(s) 523
BspEI TCCGGA 1 cut(s) 1081
BspFNI CGCG 2 cut(s) 365, 1019
BspLI GGNNCC 5 cut(s) 111, 787, 875, 1046, 1305
BspMI ACCTGC 1 cut(s) 381
BspPI GGATC 6 cut(s) 252, 410, 510, 542, 765, 1289
BspTNI GGTCTC 2 cut(s) 398, 785
BsrBI CCGCTC 2 cut(s) 1168, 1378
BsrFI RCCGGY 1 cut(s) 464
BsrGI TGTACA 1 cut(s) 157
BsrI ACTGG 6 cut(s) 91, 802, 877, 1055, 1280, 1305
BssAI RCCGGY 1 cut(s) 464
BssECI CCNNGG 4 cut(s) 231, 651, 669, 1040
BssMI GATC 9 cut(s) 244, 415, 502, 547, 706, 726, 757, 1270, 1294
BssT1I CCWWGG 2 cut(s) 651, 1040
Bst2UI CCWGG 5 cut(s) 126, 233, 986, 1158, 1308
Bst4CI ACNGT 6 cut(s) 169, 199, 226, 574, 598, 629
BstAUI TGTACA 1 cut(s) 157
BstC8I GCNNGC 4 cut(s) 452, 646, 1089, 1317
BstDEI CTNAG 2 cut(s) 15, 531
BstF5I GGATG 5 cut(s) 183, 211, 298, 966, 1338
BstFNI CGCG 2 cut(s) 365, 1019
BstKTI GATC 9 cut(s) 247, 418, 505, 550, 709, 729, 760, 1273, 1297
BstMAI GTCTC 6 cut(s) 398, 562, 563, 615, 635, 785
BstMBI GATC 9 cut(s) 244, 415, 502, 547, 706, 726, 757, 1270, 1294
BstMCI CGRYCG 1 cut(s) 201
BstMWI GCNNNNNNNGC 2 cut(s) 337, 1107
BstNI CCWGG 5 cut(s) 126, 233, 986, 1158, 1308
BstNSI RCATGY 1 cut(s) 823
BstSCI CCNGG 5 cut(s) 124, 231, 984, 1156, 1306
BstSFI CTRYAG 1 cut(s) 1105
BstSLI GKGCMC 1 cut(s) 1134
BstUI CGCG 2 cut(s) 365, 1019
BstV2I GAAGAC 3 cut(s) 58, 519, 656
BstX2I RGATCY 3 cut(s) 502, 726, 757
BstXI CCANNNNNNTGG 2 cut(s) 730, 977
BstYI RGATCY 3 cut(s) 502, 726, 757
BsuRI GGCC 5 cut(s) 687, 697, 884, 984, 1156
BtgZI GCGATG 1 cut(s) 957
BtrI CACGTC 1 cut(s) 1135
BtsCI GGATG 5 cut(s) 183, 211, 298, 966, 1338
BtsIMutI CAGTG 6 cut(s) 222, 359, 795, 884, 1273, 1298
BveI ACCTGC 1 cut(s) 381
Cac8I GCNNGC 4 cut(s) 452, 646, 1089, 1317
Cfr10I RCCGGY 1 cut(s) 464
Cfr13I GGNCC 6 cut(s) 110, 165, 695, 785, 800, 882
CseI GACGC 3 cut(s) 354, 389, 1008
Csp6I GTAC 5 cut(s) 158, 432, 594, 838, 1387
CviAII CATG 7 cut(s) 106, 520, 637, 808, 820, 916, 956
CviQI GTAC 5 cut(s) 158, 432, 594, 838, 1387
DdeI CTNAG 2 cut(s) 15, 531
DpnI GATC 9 cut(s) 246, 417, 504, 549, 708, 728, 759, 1272, 1296
DpnII GATC 9 cut(s) 244, 415, 502, 547, 706, 726, 757, 1270, 1294
DriI GACNNNNNGTC 1 cut(s) 798
EaeI YGGCCR 2 cut(s) 685, 982
Eam1105I GACNNNNNGTC 1 cut(s) 798
EciI GGCGGA 2 cut(s) 540, 749
Eco130I CCWWGG 2 cut(s) 651, 1040
Eco147I AGGCCT 1 cut(s) 1156
Eco31I GGTCTC 2 cut(s) 398, 785
Eco47I GGWCC 4 cut(s) 110, 165, 785, 800
Eco88I CYCGRG 2 cut(s) 668, 788
EcoRII CCWGG 5 cut(s) 124, 231, 984, 1156, 1306
EcoT14I CCWWGG 2 cut(s) 651, 1040
ErhI CCWWGG 2 cut(s) 651, 1040
Esp3I CGTCTC 2 cut(s) 562, 635
FaeI CATG 7 cut(s) 109, 523, 640, 811, 823, 919, 959
FaqI GGGAC 3 cut(s) 764, 771, 1006
FatI CATG 7 cut(s) 105, 519, 636, 807, 819, 915, 955
FauI CCCGC 1 cut(s) 1383
FbaI TGATCA 1 cut(s) 1270
FblI GTMKAC 2 cut(s) 83, 816
Fnu4HI GCNGC 1 cut(s) 1099
FokI GGATG 5 cut(s) 190, 218, 305, 953, 1325
Fsp4HI GCNGC 1 cut(s) 1099
FspBI CTAG 1 cut(s) 1218
GluI GCNGC 1 cut(s) 1099
GsaI CCCAGC 2 cut(s) 873, 1027
GsuI CTGGAG 1 cut(s) 147
HaeIII GGCC 5 cut(s) 687, 697, 884, 984, 1156
HapII CCGG 3 cut(s) 465, 1082, 1242
HgaI GACGC 3 cut(s) 354, 389, 1008
Hin1II CATG 7 cut(s) 109, 523, 640, 811, 823, 919, 959
HincII GTYRAC 1 cut(s) 817
HindII GTYRAC 1 cut(s) 817
HinfI GANTC 8 cut(s) 22, 45, 102, 443, 715, 857, 1202, 1212
HpaII CCGG 3 cut(s) 465, 1082, 1242
HphI GGTGA 4 cut(s) 247, 290, 473, 485
Hpy166II GTNNAC 3 cut(s) 84, 476, 817
Hpy188I TCNGA 9 cut(s) 34, 101, 370, 415, 547, 1122, 1152, 1294, 1384
Hpy188III TCNNGA 5 cut(s) 242, 668, 712, 788, 1082
Hpy8I GTNNAC 3 cut(s) 84, 476, 817
HpyCH4III ACNGT 6 cut(s) 169, 199, 226, 574, 598, 629
HpyCH4IV ACGT 1 cut(s) 1134
HpyCH4V TGCA 5 cut(s) 29, 357, 508, 764, 899
HpyF10VI GCNNNNNNNGC 2 cut(s) 337, 1107
HpyF3I CTNAG 2 cut(s) 15, 531
HpySE526I ACGT 1 cut(s) 1134
Hsp92II CATG 7 cut(s) 109, 523, 640, 811, 823, 919, 959
Kpn2I TCCGGA 1 cut(s) 1081
Ksp22I TGATCA 1 cut(s) 1270
Kzo9I GATC 9 cut(s) 244, 415, 502, 547, 706, 726, 757, 1270, 1294
LmnI GCTCC 4 cut(s) 527, 1050, 1084, 1363
LweI GCATC 4 cut(s) 38, 283, 952, 1110
MaeI CTAG 1 cut(s) 1218
MaeII ACGT 1 cut(s) 1134
MalI GATC 9 cut(s) 246, 417, 504, 549, 708, 728, 759, 1272, 1296
MbiI CCGCTC 2 cut(s) 1168, 1378
MboI GATC 9 cut(s) 244, 415, 502, 547, 706, 726, 757, 1270, 1294
MboII GAAGA 6 cut(s) 58, 145, 163, 524, 656, 920
MflI RGATCY 3 cut(s) 502, 726, 757
MhlI GDGCHC 2 cut(s) 456, 1134
MlsI TGGCCA 1 cut(s) 984
MluCI AATT 4 cut(s) 115, 248, 459, 1146
MluI ACGCGT 1 cut(s) 1017
MluNI TGGCCA 1 cut(s) 984
MlyI GAGTC 1 cut(s) 452
MmeI TCCRAC 1 cut(s) 1019
Mox20I TGGCCA 1 cut(s) 984
MroI TCCGGA 1 cut(s) 1081
MroXI GAANNNNTTC 1 cut(s) 663
MscI TGGCCA 1 cut(s) 984
MseI TTAA 3 cut(s) 843, 1116, 1226
MslI CAYNNNNRTG 3 cut(s) 176, 960, 975
Msp20I TGGCCA 1 cut(s) 984
MspI CCGG 3 cut(s) 465, 1082, 1242
MspR9I CCNGG 5 cut(s) 126, 233, 986, 1158, 1308
Mva1269I GAATGC 1 cut(s) 268
MvaI CCWGG 5 cut(s) 126, 233, 986, 1158, 1308
MvnI CGCG 2 cut(s) 365, 1019
MwoI GCNNNNNNNGC 2 cut(s) 337, 1107
NdeII GATC 9 cut(s) 244, 415, 502, 547, 706, 726, 757, 1270, 1294
NlaIII CATG 7 cut(s) 109, 523, 640, 811, 823, 919, 959
NlaIV GGNNCC 5 cut(s) 111, 787, 875, 1046, 1305
NspI RCATGY 1 cut(s) 823
OliI CACNNNNGTG 1 cut(s) 975
PceI AGGCCT 1 cut(s) 1156
PciI ACATGT 1 cut(s) 819
PctI GAATGC 1 cut(s) 268
PdmI GAANNNNTTC 1 cut(s) 663
PfeI GAWTC 7 cut(s) 22, 45, 102, 715, 857, 1202, 1212
PfoI TCCNGGA 1 cut(s) 124
PkrI GCNGC 1 cut(s) 1100
PleI GAGTC 1 cut(s) 451
PpsI GAGTC 1 cut(s) 451
PscI ACATGT 1 cut(s) 819
PsiI TTATAA 1 cut(s) 539
Psp6I CCWGG 5 cut(s) 124, 231, 984, 1156, 1306
PspFI CCCAGC 2 cut(s) 869, 1023
PspGI CCWGG 5 cut(s) 124, 231, 984, 1156, 1306
PspN4I GGNNCC 5 cut(s) 111, 787, 875, 1046, 1305
PspPI GGNCC 6 cut(s) 110, 165, 695, 785, 800, 882
PsuI RGATCY 3 cut(s) 502, 726, 757
RsaI GTAC 5 cut(s) 159, 433, 595, 839, 1388
RsaNI GTAC 5 cut(s) 158, 432, 594, 838, 1387
RseI CAYNNNNRTG 3 cut(s) 176, 960, 975
SalI GTCGAC 1 cut(s) 815
SaqAI TTAA 3 cut(s) 843, 1116, 1226
SatI GCNGC 1 cut(s) 1099
Sau3AI GATC 9 cut(s) 244, 415, 502, 547, 706, 726, 757, 1270, 1294
Sau96I GGNCC 6 cut(s) 110, 165, 695, 785, 800, 882
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 5 cut(s) 126, 233, 986, 1158, 1308
SduI GDGCHC 2 cut(s) 456, 1134
SfaNI GCATC 4 cut(s) 38, 283, 952, 1110
SfcI CTRYAG 1 cut(s) 1105
SinI GGWCC 4 cut(s) 110, 165, 785, 800
SmiMI CAYNNNNRTG 3 cut(s) 176, 960, 975
SmlI CTYRAG 3 cut(s) 395, 578, 710
SmoI CTYRAG 3 cut(s) 395, 578, 710
Sse9I AATT 4 cut(s) 115, 248, 459, 1146
SseBI AGGCCT 1 cut(s) 1156
SsiI CCGC 6 cut(s) 525, 734, 1098, 1166, 1342, 1376
SspMI CTAG 1 cut(s) 1218
StuI AGGCCT 1 cut(s) 1156
StyD4I CCNGG 5 cut(s) 124, 231, 984, 1156, 1306
StyI CCWWGG 2 cut(s) 651, 1040
TaaI ACNGT 6 cut(s) 169, 199, 226, 574, 598, 629
TaiI ACGT 1 cut(s) 1137
TaqI TCGA 5 cut(s) 201, 486, 816, 1003, 1068
TasI AATT 4 cut(s) 115, 248, 459, 1146
TatI WGTACW 1 cut(s) 157
TauI GCSGC 1 cut(s) 1101
TfiI GAWTC 7 cut(s) 22, 45, 102, 715, 857, 1202, 1212
Tru1I TTAA 3 cut(s) 843, 1116, 1226
Tru9I TTAA 3 cut(s) 843, 1116, 1226
TscAI CASTG 6 cut(s) 229, 359, 802, 884, 1280, 1305
TspDTI ATGAA 3 cut(s) 329, 508, 861
TspGWI ACGGA 2 cut(s) 554, 850
TspRI CASTG 6 cut(s) 229, 359, 802, 884, 1280, 1305
VpaK11BI GGWCC 4 cut(s) 110, 165, 785, 800
XapI RAATTY 2 cut(s) 115, 1146
XceI RCATGY 1 cut(s) 823
XcmI CCANNNNNNNNNTGG 1 cut(s) 977
XmiI GTMKAC 2 cut(s) 83, 816
XmnI GAANNNNTTC 1 cut(s) 663
XspI CTAG 1 cut(s) 1218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.