Rh7BG279100

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001995
Physical Location & Seq
Reverse (-)
25922936 .. 25924043
1108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG279100.1

Sequence Viewer

Length: 711 bp
ATGGTGAAGTTTACAGCTGAGGAGCTTCGCCGGATTATGGACTACAAACACAACATTCGCAATATGTCTGTTATTGCACATGTTGATCACGGGAAGTCAACCCTTACGGATTCTCTTGTGGCTGCTGCTGGTATCATTGCCCAAGAGGTTGCTGGTGATGTTCGGATGACAGATACCCGCGCTGATGAGGCAGAGCGTGGCATCACAATCAAATCTACTGGTATCTCACTCTATTATGAAATGTCTGATGATGCTCTGAAGAGCTACAAGAGAGAAAGAAACGGAAATGAGTACCTCATCAACCTCATTGATTCCCCCGGGCACGTTGACTTTTCATCTGAGGTCACAGCTGCTCTTCGCATTACTGATGGTGCACTTGTGGTCGTTGATTGCATTGAGGGCGTTTGTGTACAAACAGAGACTGTGCTCCGTCAAGCCTTGGGAGAAGGGATCAGGCCTGTTTTGACTGTTAACAAGATGGACAGGTGCTTCCTTGAGCTCCAGGTGGATGGAGAGGAGGCGTACCAAGCATTTCAAAGGGTTATTGAGAATGCTAATGTCATTATGGCTACCTACGAAGACGCCCTCCTTGGTGATGTCCAGGTCTACCCTGAGAAAGGAACGGTTGCTTTCTCTGCTGGCCGGTTTGCATGGTTGGGCTTTCACTCTAACCAACTTTGCCAAGATGTATGCATCCAAGTTTGGAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04152 ko04921 map04152 map04921
Pfam Domains
Protein Families
EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

236

Amino Acids

26.19

Weight (kDa)

4.79

Isoelectric Point (pI)

33.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 18 - 184 7.8e-49 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 606
AccII CGCG 1 cut(s) 180
AciI CCGC 1 cut(s) 178
AclWI GGATC 1 cut(s) 458
AcoI YGGCCR 1 cut(s) 640
AcuI CTGAAG 1 cut(s) 278
AcyI GRCGYC 1 cut(s) 582
AfaI GTAC 3 cut(s) 293, 411, 524
AfiI CCNNNNNNNGG 2 cut(s) 37, 617
AflIII ACRYGT 1 cut(s) 79
AgsI TTSAA 1 cut(s) 536
AjnI CCWGG 2 cut(s) 501, 600
AluBI AGCT 5 cut(s) 17, 25, 264, 350, 499
AluI AGCT 5 cut(s) 17, 25, 264, 350, 499
Alw21I GWGCWC 3 cut(s) 376, 429, 501
Alw26I GTCTC 1 cut(s) 413
Alw44I GTGCAC 1 cut(s) 372
AlwI GGATC 1 cut(s) 458
AlwNI CAGNNNCTG 1 cut(s) 422
Ama87I CYCGRG 1 cut(s) 317
AoxI GGCC 2 cut(s) 455, 640
ApaLI GTGCAC 1 cut(s) 372
ApeKI GCWGC 3 cut(s) 122, 125, 350
AspLEI GCGC 1 cut(s) 182
AsuC2I CCSGG 2 cut(s) 318, 319
AsuHPI GGTGA 3 cut(s) 16, 167, 605
AvaI CYCGRG 1 cut(s) 317
BaeGI GKGCMC 2 cut(s) 324, 376
BanII GRGCYC 1 cut(s) 501
BbsI GAAGAC 1 cut(s) 585
Bbv12I GWGCWC 3 cut(s) 376, 429, 501
BbvCI CCTCAGC 1 cut(s) 18
BbvI GCAGC 3 cut(s) 109, 112, 337
BccI CCATC 3 cut(s) 362, 472, 503
BciT130I CCWGG 2 cut(s) 503, 602
BclI TGATCA 1 cut(s) 85
BcnI CCSGG 2 cut(s) 318, 319
BcoDI GTCTC 1 cut(s) 413
BisI GCNGC 3 cut(s) 123, 126, 351
BlsI GCNGC 3 cut(s) 124, 127, 352
Bme1390I CCNGG 4 cut(s) 318, 319, 503, 602
BmeT110I CYCGRG 1 cut(s) 317
BmrFI CCNGG 4 cut(s) 318, 319, 503, 602
BmsI GCATC 3 cut(s) 210, 241, 702
BpiI GAAGAC 1 cut(s) 585
BpmI CTGGAG 1 cut(s) 485
Bpu10I CCTNAGC 1 cut(s) 18
BpuEI CTTGAG 1 cut(s) 515
BpuMI CCSGG 2 cut(s) 318, 319
BsaHI GRCGYC 1 cut(s) 582
BsaJI CCNNGG 4 cut(s) 316, 317, 438, 589
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 617
Bse118I RCCGGY 1 cut(s) 642
Bse1I ACTGG 1 cut(s) 223
Bse3DI GCAATG 1 cut(s) 135
BseBI CCWGG 2 cut(s) 503, 602
BseDI CCNNGG 4 cut(s) 316, 317, 438, 589
BseGI GGATG 3 cut(s) 171, 514, 693
BseLI CCNNNNNNNGG 2 cut(s) 37, 617
BseMI GCAATG 1 cut(s) 135
BseMII CTCAG 3 cut(s) 9, 330, 603
BseNI ACTGG 1 cut(s) 223
BseRI GAGGAG 2 cut(s) 35, 530
BseSI GKGCMC 2 cut(s) 324, 376
BseXI GCAGC 3 cut(s) 109, 112, 337
Bsh1236I CGCG 1 cut(s) 180
BshFI GGCC 2 cut(s) 457, 642
BsiHKAI GWGCWC 3 cut(s) 376, 429, 501
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 3 cut(s) 31, 318, 643
BslI CCNNNNNNNGG 2 cut(s) 37, 617
BsmAI GTCTC 1 cut(s) 413
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 2 cut(s) 457, 642
BsoBI CYCGRG 1 cut(s) 317
Bsp1286I GDGCHC 4 cut(s) 324, 376, 429, 501
Bsp1407I TGTACA 1 cut(s) 409
Bsp143I GATC 2 cut(s) 85, 450
BspACI CCGC 1 cut(s) 178
BspANI GGCC 2 cut(s) 457, 642
BspCNI CTCAG 3 cut(s) 10, 331, 604
BspFNI CGCG 1 cut(s) 180
BspPI GGATC 1 cut(s) 458
BspQI GCTCTTC 2 cut(s) 254, 360
BsrDI GCAATG 1 cut(s) 135
BsrFI RCCGGY 1 cut(s) 642
BsrGI TGTACA 1 cut(s) 409
BsrI ACTGG 1 cut(s) 223
BssAI RCCGGY 1 cut(s) 642
BssECI CCNNGG 4 cut(s) 316, 317, 438, 589
BssMI GATC 2 cut(s) 85, 450
BssNI GRCGYC 1 cut(s) 582
BssT1I CCWWGG 2 cut(s) 438, 589
Bst2UI CCWGG 2 cut(s) 503, 602
Bst4CI ACNGT 3 cut(s) 424, 469, 625
Bst6I CTCTTC 2 cut(s) 254, 360
BstACI GRCGYC 1 cut(s) 582
BstAUI TGTACA 1 cut(s) 409
BstC8I GCNNGC 1 cut(s) 640
BstDEI CTNAG 3 cut(s) 18, 339, 612
BstENI CCTNNNNNAGG 1 cut(s) 615
BstF5I GGATG 3 cut(s) 171, 514, 693
BstFNI CGCG 1 cut(s) 180
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 2 cut(s) 88, 453
BstMAI GTCTC 1 cut(s) 413
BstMBI GATC 2 cut(s) 85, 450
BstMWI GCNNNNNNNGC 4 cut(s) 188, 399, 527, 635
BstNI CCWGG 2 cut(s) 503, 602
BstNSI RCATGY 1 cut(s) 83
BstSCI CCNGG 4 cut(s) 316, 317, 501, 600
BstSLI GKGCMC 2 cut(s) 324, 376
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 3 cut(s) 109, 112, 337
BstV2I GAAGAC 1 cut(s) 585
BstXI CCANNNNNNTGG 1 cut(s) 509
BsuRI GGCC 2 cut(s) 457, 642
BtsCI GGATG 3 cut(s) 171, 514, 693
Cac8I GCNNGC 1 cut(s) 640
CaiI CAGNNNCTG 1 cut(s) 422
CfoI GCGC 1 cut(s) 182
Cfr10I RCCGGY 1 cut(s) 642
Cfr9I CCCGGG 1 cut(s) 317
CseI GACGC 1 cut(s) 590
Csp6I GTAC 3 cut(s) 292, 410, 523
CviAII CATG 2 cut(s) 80, 651
CviQI GTAC 3 cut(s) 292, 410, 523
DdeI CTNAG 3 cut(s) 18, 339, 612
DpnI GATC 2 cut(s) 87, 452
DpnII GATC 2 cut(s) 85, 450
EaeI YGGCCR 1 cut(s) 640
Eam1104I CTCTTC 2 cut(s) 254, 360
EarI CTCTTC 2 cut(s) 254, 360
Ecl136II GAGCTC 1 cut(s) 499
Eco130I CCWWGG 2 cut(s) 438, 589
Eco147I AGGCCT 1 cut(s) 457
Eco24I GRGCYC 1 cut(s) 501
Eco53kI GAGCTC 1 cut(s) 499
Eco57I CTGAAG 1 cut(s) 278
Eco88I CYCGRG 1 cut(s) 317
EcoICRI GAGCTC 1 cut(s) 499
EcoNI CCTNNNNNAGG 1 cut(s) 615
EcoRII CCWGG 2 cut(s) 501, 600
EcoT14I CCWWGG 2 cut(s) 438, 589
EcoT22I ATGCAT 1 cut(s) 695
EcoT38I GRGCYC 1 cut(s) 501
ErhI CCWWGG 2 cut(s) 438, 589
FaeI CATG 2 cut(s) 83, 654
FaiI YATR 7 cut(s) 38, 65, 81, 237, 566, 652, 691
FatI CATG 2 cut(s) 79, 650
FauI CCCGC 1 cut(s) 185
FbaI TGATCA 1 cut(s) 85
FblI GTMKAC 1 cut(s) 606
Fnu4HI GCNGC 3 cut(s) 123, 126, 351
FokI GGATG 3 cut(s) 178, 521, 680
FriOI GRGCYC 1 cut(s) 501
Fsp4HI GCNGC 3 cut(s) 123, 126, 351
GlaI GCGC 1 cut(s) 181
GluI GCNGC 3 cut(s) 123, 126, 351
GsuI CTGGAG 1 cut(s) 485
HaeIII GGCC 2 cut(s) 457, 642
HapII CCGG 3 cut(s) 31, 318, 643
HgaI GACGC 1 cut(s) 590
HhaI GCGC 1 cut(s) 182
Hin1I GRCGYC 1 cut(s) 582
Hin1II CATG 2 cut(s) 83, 654
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HincII GTYRAC 3 cut(s) 99, 328, 472
HindII GTYRAC 3 cut(s) 99, 328, 472
HinfI GANTC 2 cut(s) 110, 311
HpaI GTTAAC 1 cut(s) 472
HpaII CCGG 3 cut(s) 31, 318, 643
HphI GGTGA 3 cut(s) 16, 167, 605
Hpy166II GTNNAC 7 cut(s) 12, 99, 328, 374, 410, 472, 607
Hpy188I TCNGA 4 cut(s) 165, 247, 258, 340
Hpy8I GTNNAC 7 cut(s) 12, 99, 328, 374, 410, 472, 607
HpyAV CCTTC 1 cut(s) 440
HpyCH4III ACNGT 3 cut(s) 424, 469, 625
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 5 cut(s) 77, 374, 393, 650, 693
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 399, 527, 635
HpyF3I CTNAG 3 cut(s) 18, 339, 612
HpySE526I ACGT 1 cut(s) 324
Hsp92I GRCGYC 1 cut(s) 582
Hsp92II CATG 2 cut(s) 83, 654
HspAI GCGC 1 cut(s) 180
Ksp22I TGATCA 1 cut(s) 85
KspAI GTTAAC 1 cut(s) 472
Kzo9I GATC 2 cut(s) 85, 450
LguI GCTCTTC 2 cut(s) 254, 360
LmnI GCTCC 3 cut(s) 22, 432, 504
Lsp1109I GCAGC 3 cut(s) 109, 112, 337
LweI GCATC 3 cut(s) 210, 241, 702
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 1 cut(s) 343
MalI GATC 2 cut(s) 87, 452
MboI GATC 2 cut(s) 85, 450
MboII GAAGA 3 cut(s) 271, 347, 590
MhlI GDGCHC 4 cut(s) 324, 376, 429, 501
Mph1103I ATGCAT 1 cut(s) 695
MseI TTAA 1 cut(s) 471
MspA1I CMGCKG 2 cut(s) 17, 350
MspI CCGG 3 cut(s) 31, 318, 643
MspR9I CCNGG 4 cut(s) 318, 319, 503, 602
Mva1269I GAATGC 1 cut(s) 556
MvaI CCWGG 2 cut(s) 503, 602
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 4 cut(s) 188, 399, 527, 635
NciI CCSGG 2 cut(s) 318, 319
NdeII GATC 2 cut(s) 85, 450
NlaIII CATG 2 cut(s) 83, 654
NmuCI GTSAC 1 cut(s) 343
NsiI ATGCAT 1 cut(s) 695
NspI RCATGY 1 cut(s) 83
PceI AGGCCT 1 cut(s) 457
PciI ACATGT 1 cut(s) 79
PciSI GCTCTTC 2 cut(s) 254, 360
PctI GAATGC 1 cut(s) 556
PfeI GAWTC 2 cut(s) 110, 311
PkrI GCNGC 3 cut(s) 124, 127, 352
PscI ACATGT 1 cut(s) 79
Psp124BI GAGCTC 1 cut(s) 501
Psp6I CCWGG 2 cut(s) 501, 600
PspGI CCWGG 2 cut(s) 501, 600
PstNI CAGNNNCTG 1 cut(s) 422
PvuII CAGCTG 2 cut(s) 17, 350
RsaI GTAC 3 cut(s) 293, 411, 524
RsaNI GTAC 3 cut(s) 292, 410, 523
SacI GAGCTC 1 cut(s) 501
SapI GCTCTTC 2 cut(s) 254, 360
SaqAI TTAA 1 cut(s) 471
SatI GCNGC 3 cut(s) 123, 126, 351
Sau3AI GATC 2 cut(s) 85, 450
ScrFI CCNGG 4 cut(s) 318, 319, 503, 602
SduI GDGCHC 4 cut(s) 324, 376, 429, 501
SfaNI GCATC 3 cut(s) 210, 241, 702
SmaI CCCGGG 1 cut(s) 319
SmlI CTYRAG 1 cut(s) 494
SmoI CTYRAG 1 cut(s) 494
SseBI AGGCCT 1 cut(s) 457
SsiI CCGC 1 cut(s) 178
SstI GAGCTC 1 cut(s) 501
StuI AGGCCT 1 cut(s) 457
StyD4I CCNGG 4 cut(s) 316, 317, 501, 600
StyI CCWWGG 2 cut(s) 438, 589
TaaI ACNGT 3 cut(s) 424, 469, 625
TaiI ACGT 1 cut(s) 327
TatI WGTACW 1 cut(s) 409
TfiI GAWTC 2 cut(s) 110, 311
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TseFI GTSAC 1 cut(s) 343
TseI GCWGC 3 cut(s) 122, 125, 350
Tsp45I GTSAC 1 cut(s) 343
TspDTI ATGAA 2 cut(s) 252, 324
TspGWI ACGGA 3 cut(s) 122, 297, 419
TspMI CCCGGG 1 cut(s) 317
VneI GTGCAC 1 cut(s) 372
XagI CCTNNNNNAGG 1 cut(s) 615
XceI RCATGY 1 cut(s) 83
XcmI CCANNNNNNNNNTGG 1 cut(s) 149
XmaI CCCGGG 1 cut(s) 317
XmiI GTMKAC 1 cut(s) 606
Zsp2I ATGCAT 1 cut(s) 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.