Rorug07G0150400

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001995
Physical Location & Seq
Reverse (-)
11938978 .. 11940279
1302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0150400.1

Sequence Viewer

Length: 270 bp
ATGGACGCTTCTAAGGCCCTAATATTGGGGGCAAGCGGTGCTGTTAATGGCATCATGTTGCTTGATATATTCCTTCACCCCACAGCTACCCTCTACTTTGATTTTATCATACCAGTTCCTGCCATGCTGCTGGGAATCTTTCTAATTGGAAAGGATATCTTAAGAATAATGGAGGGAAACAGTCAGATCTCAGGATCTGCACACTGGGGTGGTGCGGCAGCAGCAGCCATAGCCTGGGCAAGACTTCGAAAGGGGCGTGGTTTATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04152 ko04921 map04152 map04921
Pfam Domains
Protein Families
EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

89

Amino Acids

9.4

Weight (kDa)

9.52

Isoelectric Point (pI)

14.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 7 - 80 1.1e-08 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 234
AciI CCGC 2 cut(s) 36, 215
AclWI GGATC 1 cut(s) 202
AfiI CCNNNNNNNGG 2 cut(s) 25, 234
AflII CTTAAG 1 cut(s) 160
AjnI CCWGG 1 cut(s) 233
AleI CACNNNNGTG 1 cut(s) 207
AluBI AGCT 1 cut(s) 86
AluI AGCT 1 cut(s) 86
AlwI GGATC 1 cut(s) 202
AlwNI CAGNNNCTG 2 cut(s) 119, 197
AoxI GGCC 1 cut(s) 15
ApeKI GCWGC 4 cut(s) 127, 218, 221, 224
AspS9I GGNCC 1 cut(s) 16
AsuHPI GGTGA 1 cut(s) 68
AsuII TTCGAA 1 cut(s) 247
BbvI GCAGC 4 cut(s) 114, 230, 233, 236
BciT130I CCWGG 1 cut(s) 235
BfaI CTAG 1 cut(s) 268
BfrI CTTAAG 1 cut(s) 160
BglII AGATCT 1 cut(s) 186
BisI GCNGC 5 cut(s) 128, 216, 219, 222, 225
BlsI GCNGC 5 cut(s) 129, 217, 220, 223, 226
Bme1390I CCNGG 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 16
BmrFI CCNGG 1 cut(s) 235
BmrI ACTGGG 1 cut(s) 214
BmsI GCATC 1 cut(s) 60
BmuI ACTGGG 1 cut(s) 214
Bpu14I TTCGAA 1 cut(s) 247
BsaJI CCNNGG 1 cut(s) 234
Bsc4I CCNNNNNNNGG 2 cut(s) 25, 234
Bse1I ACTGG 2 cut(s) 113, 209
BseBI CCWGG 1 cut(s) 235
BseDI CCNNGG 1 cut(s) 234
BseLI CCNNNNNNNGG 2 cut(s) 25, 234
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 2 cut(s) 113, 209
BseXI GCAGC 4 cut(s) 114, 230, 233, 236
BseYI CCCAGC 1 cut(s) 130
BsgI GTGCAG 1 cut(s) 183
BshFI GGCC 1 cut(s) 17
BslI CCNNNNNNNGG 2 cut(s) 25, 234
BsnI GGCC 1 cut(s) 17
Bsp119I TTCGAA 1 cut(s) 247
Bsp143I GATC 2 cut(s) 186, 194
BspACI CCGC 2 cut(s) 36, 215
BspANI GGCC 1 cut(s) 17
BspCNI CTCAG 1 cut(s) 203
BspPI GGATC 1 cut(s) 202
BspT104I TTCGAA 1 cut(s) 247
BspTI CTTAAG 1 cut(s) 160
BsrI ACTGG 2 cut(s) 113, 209
BssECI CCNNGG 1 cut(s) 234
BssMI GATC 2 cut(s) 186, 194
Bst2UI CCWGG 1 cut(s) 235
Bst4CI ACNGT 1 cut(s) 182
BstAFI CTTAAG 1 cut(s) 160
BstAPI GCANNNNNTGC 1 cut(s) 38
BstBI TTCGAA 1 cut(s) 247
BstC8I GCNNGC 1 cut(s) 34
BstDEI CTNAG 2 cut(s) 12, 190
BstKTI GATC 2 cut(s) 189, 197
BstMBI GATC 2 cut(s) 186, 194
BstMWI GCNNNNNNNGC 5 cut(s) 14, 38, 221, 224, 230
BstNI CCWGG 1 cut(s) 235
BstSCI CCNGG 1 cut(s) 233
BstV1I GCAGC 4 cut(s) 114, 230, 233, 236
BstX2I RGATCY 2 cut(s) 186, 194
BstXI CCANNNNNNTGG 1 cut(s) 130
BstYI RGATCY 2 cut(s) 186, 194
BsuRI GGCC 1 cut(s) 17
BtsIMutI CAGTG 1 cut(s) 202
Cac8I GCNNGC 1 cut(s) 34
CaiI CAGNNNCTG 2 cut(s) 119, 197
Cfr13I GGNCC 1 cut(s) 16
CseI GACGC 1 cut(s) 14
CviAII CATG 2 cut(s) 55, 124
CviJI RGCY 4 cut(s) 17, 86, 227, 233
CviKI_1 RGCY 4 cut(s) 17, 86, 227, 233
DdeI CTNAG 2 cut(s) 12, 190
DpnI GATC 2 cut(s) 188, 196
DpnII GATC 2 cut(s) 186, 194
Eco32I GATATC 1 cut(s) 157
EcoO109I RGGNCCY 1 cut(s) 16
EcoRII CCWGG 1 cut(s) 233
EcoRV GATATC 1 cut(s) 157
FaeI CATG 2 cut(s) 58, 127
FaiI YATR 5 cut(s) 56, 68, 110, 125, 230
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FatI CATG 2 cut(s) 54, 123
Fnu4HI GCNGC 5 cut(s) 128, 216, 219, 222, 225
Fsp4HI GCNGC 5 cut(s) 128, 216, 219, 222, 225
FspBI CTAG 1 cut(s) 268
GluI GCNGC 5 cut(s) 128, 216, 219, 222, 225
GsaI CCCAGC 1 cut(s) 134
HaeIII GGCC 1 cut(s) 17
HgaI GACGC 1 cut(s) 14
Hin1II CATG 2 cut(s) 58, 127
HinfI GANTC 1 cut(s) 135
HphI GGTGA 1 cut(s) 68
Hpy188I TCNGA 1 cut(s) 186
Hpy188III TCNNGA 1 cut(s) 192
HpyAV CCTTC 1 cut(s) 83
HpyCH4III ACNGT 1 cut(s) 182
HpyCH4V TGCA 1 cut(s) 200
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 38, 221, 224, 230
HpyF3I CTNAG 2 cut(s) 12, 190
Hsp92II CATG 2 cut(s) 58, 127
Kzo9I GATC 2 cut(s) 186, 194
LpnPI CCDG 7 cut(s) 116, 126, 132, 177, 190, 220, 247
Lsp1109I GCAGC 4 cut(s) 114, 230, 233, 236
LweI GCATC 1 cut(s) 60
MaeI CTAG 1 cut(s) 268
MalI GATC 2 cut(s) 188, 196
MboI GATC 2 cut(s) 186, 194
MflI RGATCY 2 cut(s) 186, 194
MluCI AATT 1 cut(s) 144
MnlI CCTC 2 cut(s) 101, 166
MseI TTAA 2 cut(s) 45, 161
MslI CAYNNNNRTG 1 cut(s) 207
MspCI CTTAAG 1 cut(s) 160
MspR9I CCNGG 1 cut(s) 235
MvaI CCWGG 1 cut(s) 235
MwoI GCNNNNNNNGC 5 cut(s) 14, 38, 221, 224, 230
NdeII GATC 2 cut(s) 186, 194
NlaIII CATG 2 cut(s) 58, 127
NspV TTCGAA 1 cut(s) 247
OliI CACNNNNGTG 1 cut(s) 207
PcsI WCGNNNNNNNCGW 1 cut(s) 253
PfeI GAWTC 1 cut(s) 135
PflMI CCANNNNNTGG 1 cut(s) 234
PkrI GCNGC 5 cut(s) 129, 217, 220, 223, 226
Psp6I CCWGG 1 cut(s) 233
PspFI CCCAGC 1 cut(s) 130
PspGI CCWGG 1 cut(s) 233
PspPI GGNCC 1 cut(s) 16
PstNI CAGNNNCTG 2 cut(s) 119, 197
PsuI RGATCY 2 cut(s) 186, 194
RseI CAYNNNNRTG 1 cut(s) 207
SaqAI TTAA 2 cut(s) 45, 161
SatI GCNGC 5 cut(s) 128, 216, 219, 222, 225
Sau3AI GATC 2 cut(s) 186, 194
Sau96I GGNCC 1 cut(s) 16
ScrFI CCNGG 1 cut(s) 235
SetI ASST 1 cut(s) 88
SfaNI GCATC 1 cut(s) 60
SfuI TTCGAA 1 cut(s) 247
SmiMI CAYNNNNRTG 1 cut(s) 207
SmlI CTYRAG 1 cut(s) 160
SmoI CTYRAG 1 cut(s) 160
Sse9I AATT 1 cut(s) 144
SsiI CCGC 2 cut(s) 36, 215
SspI AATATT 1 cut(s) 24
SspMI CTAG 1 cut(s) 268
StyD4I CCNGG 1 cut(s) 233
TaaI ACNGT 1 cut(s) 182
TaqI TCGA 1 cut(s) 247
TasI AATT 1 cut(s) 144
TauI GCSGC 1 cut(s) 218
TfiI GAWTC 1 cut(s) 135
Tru1I TTAA 2 cut(s) 45, 161
Tru9I TTAA 2 cut(s) 45, 161
TscAI CASTG 1 cut(s) 209
TseI GCWGC 4 cut(s) 127, 218, 221, 224
TspRI CASTG 1 cut(s) 209
Van91I CCANNNNNTGG 1 cut(s) 234
Vha464I CTTAAG 1 cut(s) 160
XspI CTAG 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.