MD05G1220800.v1.1

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001995
Physical Location & Seq
Reverse (-)
35338583 .. 35354352
15770 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1220800.v1.1.491

Sequence Viewer

Length: 2535 bp
ATGGTGAAGTTTACTGCAGAGGAACTCCGTAGAATTATGGACTACAAGCACAACATTCGCAATATGTCTGTTATTGCTCATGTCGATCATGGGAAGTCGACCCTTACCGACTCGCTTGTTGCTGCTGCGGGTATCATTGCACAAGAAGTTGCTGGTGATGTCCGGATGACTGATACCCGTGCAGATGAGGCAGAGCGTGGTATCACAATTAAATCTACCGGTATCTCTCTCTACTATGAGATGAGTGATGAAGCTTTGAAGAGCTACAAGGGAGAGAGGGACGGAAATGAGTACCTCATCAATCTCATTGACTCTCCCGGGCATGTTGACTTTTCGTCTGAAGTCACAGCTGCTCTTCGTATTACTGATGGTGCACTTGTGGTGGTGGATTGTATTGAGGGTGTGTGCGTCCAGACTGAGACTGTGCTCCGTCAAGCCTTGGGAGAAAGGATCAGGCCTGTTTTGACTGTTAACAAGATGGACAGGTGTTTCCTTGAGCTCCAGGTCGATGGAGAAGAGGCTTACCAGACATTCCAAAGGGTTATTGAGAATGCCAATGTCATTATGGCTACATATGAAGATCCACTTCTTGGTGATGTCCAAGTCTACCCGGAGAAAGGAACTGTCGCCTTCTCAGCTGGTTTGCACGGTTGGGCTTTCACTCTGACCAACTTCGCCAAGATGTATGCTTCCAAGTTTGGAGTTGACGAGTCAAAGATGATGGAAAGGCTCTGGGGTGAGAACTTCTTTGACCCAGCTACCAAGAAATGGACGAGCAAGCACACTGGAACTGCTACCTGCAAACGCGGTTTCGTTCAGTTCTGTTATGAACCCATCAAGCAGATCATCAAGACCTGCATGAACGACCAGAAGGATAAGTTGTGGCCCATGTTGTCAAAGCTTAATGTCACCATGAAGAGTGATGAGAAGGAATTGATGGGGAAGGCGTTGATGAAGCGTGTCCTGCAGAACTGGCTTCCAGCAAGTAGTGCCCTGTTGGAAATGATGATCTTTCACCTTCCCTCACCTGCAACAGCTCAGAAGTATCGTGTTGAGAACTTGTACGAGGGTCCACTTGATGACAAATATGCCAACGCTATCCGGAACTGCGACCCTGAAGGACCTCTTATGCTCTATGTATCGAAGATGATTCCTGCATCTGACAAGGGCAGATTCTTTGCCTTTGGTCGTGTCTTCTCTGGCAGAGTGTCTACTGGTTTGAAGGTCAGAATCATGGGTCCAAATTTTGTTCCTGGAGAGAAGAAGGATTTGTATGTGAAGAATGTACAGAGGACCGTCATTTGGATGGGAAAGAAACAAGAAACAGTCGAGGATGTGCCTTGTGGCAACACTGTTGCCCTGGTTGGTCTGGATCAGTTTATCACCAAGAATGCTACCTTGACGAATGAGAAGGAAGTGGATGCTCACCCTATTCGTGCTATGAAGTTCTCTGTTTCGCCTGTTGTGCGCGTTGCTGTTCAGTGCAAAGTCGCGTCTGACCTGCCAAAACTTGTTGAAGGTCTCAAGCGTCTGTCCAAGTCCGATCCTATGGTTGTCTGTACCATTGAGGAGTCGGGGGGGGAGCACATTATTGCTGGTGCAGGTGAACTTCACCTCGAAATCTGTTTGAAGGATCTGCAAGAAGACTTCATGGGCGGAGCTGAGATTATAAAGTCTGATCCTGTCGTCTCTTTCCGTGAGACCGTCCTTGAGAAGTCCAGCCGTACAGTTATGAGCAAGTCTCCAAACAAGCACAACCGTCTCTACATGGAAGCACGCCCCTTGGAGGAAGGTCTTCCCGAGGCCATTGATGAGGGCCGTATTGGGCCAAGAGATGATCCCAAGATTCGTTCCAAGATCTTGGCGGAGGAGTTTGGTTGGGACAAGGATCTTGCAAAGAAAATCTGGTGTTTTGGTCCCGAGACGACTGGTCCTAACATGGTGGTCGACATGTGTAAGGGAGTTCAGTACCTTAATGAAATCAAGGATTCCGTTGTTGCGGGGTTCCAGTGGGCCTCAAAGGAAGGTGCATTGGCAGAAGAAAACATGAGAGGTATTTGCTTTGAAGTCTGCGATGTGGTTCTCCATGCCGATGCCATCCACAGGGGTGGTGGCCAGGTCATTCCAACCGCTCGAAGGGTCATCTACGCGTCCCAGCTCACAGCCAAGCCAAGGCTCCTTGAACCAGTCTATCTTGTCGAAATCCAAACTCCGGAGCAAGCTCTTGGCGGCATCTACAGCGTTCTTAATCAGAAGCGAGGGCACGTCTTTGAGGAAATTCAGAGGCCCGGCACTCCACTCTACAACATCAAGGCTTACCTGCCCGTCATTGAATCTTTTGGATTCTCTAGTCAGTTAAGGGCTTCTACTTCCGGTCAGGCTTTCCCACAATGCGTGTTTGATCACTGGGAGATGATGTCGTCTGATCCCCTGGAACCAGGAACGCAGGCTTCAACGCTCGTTGCCGACATCCGCAAGAGGAAGGGGTTGAAGGAGCAGATGACCCCGCTCTCCGAGTACGAGGATAAGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04152 ko04921 map04152 map04921
Pfam Domains
Protein Families
EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

845

Amino Acids

94.27

Weight (kDa)

5.91

Isoelectric Point (pI)

36.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 18 - 221 1.4e-48 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 379 - 469 1e-06 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 394 - 462 1.5e-08 Elongation factor Tu domain 2
EFG_III PF14492 487 - 550 1.4e-12 Elongation Factor G, domain III
EFG_IV PF03764 611 - 723 2e-29 Elongation factor G, domain IV
EFG_C PF00679 725 - 811 2.8e-21 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1670
AarI CACCTGC 2 cut(s) 1036, 1592
Acc36I ACCTGC 6 cut(s) 806, 863, 1036, 1509, 1592, 2328
AccB7I CCANNNNNTGG 2 cut(s) 590, 768
AccBSI CCGCTC 2 cut(s) 2132, 2509
AccI GTMKAC 4 cut(s) 98, 606, 1211, 1947
AccII CGCG 4 cut(s) 807, 1470, 1493, 2150
AccIII TCCGGA 3 cut(s) 162, 1101, 2212
AciI CCGC 9 cut(s) 128, 807, 1656, 1865, 2000, 2130, 2229, 2473, 2507
AclWI GGATC 9 cut(s) 458, 575, 1380, 1538, 1641, 1673, 1832, 1896, 2420
AcoI YGGCCR 1 cut(s) 2113
AcsI RAATTY 2 cut(s) 1243, 2277
AcuI CTGAAG 2 cut(s) 360, 1137
AfaI GTAC 7 cut(s) 293, 1064, 1287, 1561, 1726, 1970, 2519
AfiI CCNNNNNNNGG 9 cut(s) 590, 617, 768, 1302, 1786, 2103, 2136, 2172, 2212
AflIII ACRYGT 2 cut(s) 1950, 2148
AgeI ACCGGT 1 cut(s) 218
AgsI TTSAA 9 cut(s) 259, 1222, 1517, 1630, 2066, 2183, 2333, 2454, 2491
AhdI GACNNNNNGTC 2 cut(s) 334, 1929
AjiI CACGTC 1 cut(s) 2266
AjnI CCWGG 6 cut(s) 501, 1252, 1359, 2115, 2430, 2437
AleI CACNNNNGTG 1 cut(s) 2106
Alw21I GWGCWC 4 cut(s) 376, 429, 501, 1587
Alw26I GTCTC 7 cut(s) 413, 1526, 1693, 1694, 1746, 1766, 1916
Alw44I GTGCAC 1 cut(s) 372
AlwI GGATC 9 cut(s) 458, 575, 1380, 1538, 1641, 1673, 1832, 1896, 2420
Ama87I CYCGRG 3 cut(s) 317, 1799, 1919
Aor13HI TCCGGA 3 cut(s) 162, 1101, 2212
AoxI GGCC 8 cut(s) 455, 884, 1803, 1816, 1826, 2013, 2113, 2285
ApaLI GTGCAC 1 cut(s) 372
ApeKI GCWGC 3 cut(s) 122, 125, 350
ApoI RAATTY 2 cut(s) 1243, 2277
AsiGI ACCGGT 1 cut(s) 218
Asp700I GAANNNNTTC 1 cut(s) 1794
AspLEI GCGC 1 cut(s) 1470
AsuC2I CCSGG 4 cut(s) 318, 319, 611, 2289
AvaI CYCGRG 3 cut(s) 317, 1799, 1919
AvaII GGWCC 6 cut(s) 1070, 1121, 1238, 1293, 1916, 1931
BaeGI GKGCMC 3 cut(s) 376, 994, 2265
BalI TGGCCA 1 cut(s) 2115
BanII GRGCYC 1 cut(s) 501
BbsI GAAGAC 3 cut(s) 1186, 1650, 1787
Bbv12I GWGCWC 4 cut(s) 376, 429, 501, 1587
BbvI GCAGC 3 cut(s) 109, 112, 337
BccI CCATC 8 cut(s) 362, 472, 503, 715, 842, 931, 1300, 2105
BceAI ACGGC 2 cut(s) 1707, 1803
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BciT130I CCWGG 6 cut(s) 503, 1254, 1361, 2117, 2432, 2439
BclI TGATCA 1 cut(s) 2401
BcnI CCSGG 4 cut(s) 318, 319, 611, 2289
BcoDI GTCTC 7 cut(s) 413, 1526, 1693, 1694, 1746, 1766, 1916
BfaI CTAG 1 cut(s) 2349
BfmI CTRYAG 3 cut(s) 15, 965, 2236
BfuAI ACCTGC 6 cut(s) 806, 863, 1036, 1509, 1592, 2328
BglII AGATCT 1 cut(s) 1857
BisI GCNGC 4 cut(s) 123, 126, 351, 2230
BlsI GCNGC 4 cut(s) 124, 127, 352, 2231
Bme18I GGWCC 6 cut(s) 1070, 1121, 1238, 1293, 1916, 1931
BmeRI GACNNNNNGTC 2 cut(s) 334, 1929
BmeT110I CYCGRG 3 cut(s) 317, 1799, 1919
BmgBI CACGTC 1 cut(s) 2266
BmiI GGNNCC 6 cut(s) 1071, 1239, 1918, 2006, 2177, 2436
BmrI ACTGGG 1 cut(s) 2416
BmsI GCATC 4 cut(s) 1166, 1411, 2083, 2241
BmuI ACTGGG 1 cut(s) 2416
BpiI GAAGAC 3 cut(s) 1186, 1650, 1787
BplI GAGNNNNNCTC 2 cut(s) 1726, 1758
BpmI CTGGAG 2 cut(s) 485, 1275
BpuEI CTTGAG 3 cut(s) 515, 1508, 1730
BpuMI CCSGG 4 cut(s) 318, 319, 611, 2289
BsaI GGTCTC 2 cut(s) 1526, 1694
BsaJI CCNNGG 7 cut(s) 317, 438, 1359, 1782, 1800, 2171, 2430
BsaWI WCCGGW 5 cut(s) 162, 218, 1101, 2212, 2372
BsaXI ACNNNNNCTCC 2 cut(s) 2402, 2432
Bsc4I CCNNNNNNNGG 9 cut(s) 590, 617, 768, 1302, 1786, 2103, 2136, 2172, 2212
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 7 cut(s) 790, 977, 1219, 1933, 2008, 2186, 2411
Bse3DI GCAATG 1 cut(s) 135
BseAI TCCGGA 3 cut(s) 162, 1101, 2212
BseBI CCWGG 6 cut(s) 503, 1254, 1361, 2117, 2432, 2439
BseDI CCNNGG 7 cut(s) 317, 438, 1359, 1782, 1800, 2171, 2430
BseGI GGATG 6 cut(s) 171, 1311, 1339, 1426, 2097, 2469
BseLI CCNNNNNNNGG 9 cut(s) 590, 617, 768, 1302, 1786, 2103, 2136, 2172, 2212
BseMI GCAATG 1 cut(s) 135
BseMII CTCAG 4 cut(s) 408, 648, 1052, 1653
BseNI ACTGG 7 cut(s) 790, 977, 1219, 1933, 2008, 2186, 2411
BseRI GAGGAG 2 cut(s) 1583, 1883
BseSI GKGCMC 3 cut(s) 376, 994, 2265
BseXI GCAGC 3 cut(s) 109, 112, 337
BseYI CCCAGC 2 cut(s) 754, 2154
BsgI GTGCAG 2 cut(s) 201, 1620
Bsh1236I CGCG 4 cut(s) 807, 1470, 1493, 2150
BshFI GGCC 8 cut(s) 457, 886, 1805, 1818, 1828, 2015, 2115, 2287
BshTI ACCGGT 1 cut(s) 218
BsiHKAI GWGCWC 4 cut(s) 376, 429, 501, 1587
BsiHKCI CYCGRG 3 cut(s) 317, 1799, 1919
BsiSI CCGG 8 cut(s) 163, 219, 318, 611, 1102, 2213, 2289, 2373
BslFI GGGAC 4 cut(s) 293, 1895, 1902, 2137
BslI CCNNNNNNNGG 9 cut(s) 590, 617, 768, 1302, 1786, 2103, 2136, 2172, 2212
BsmAI GTCTC 7 cut(s) 413, 1526, 1693, 1694, 1746, 1766, 1916
BsmBI CGTCTC 3 cut(s) 1693, 1766, 1916
BsmFI GGGAC 4 cut(s) 293, 1895, 1902, 2137
BsmI GAATGC 2 cut(s) 556, 1396
BsnI GGCC 8 cut(s) 457, 886, 1805, 1818, 1828, 2015, 2115, 2287
Bso31I GGTCTC 2 cut(s) 1526, 1694
BsoBI CYCGRG 3 cut(s) 317, 1799, 1919
Bsp1286I GDGCHC 6 cut(s) 376, 429, 501, 994, 1587, 2265
Bsp13I TCCGGA 3 cut(s) 162, 1101, 2212
Bsp1407I TGTACA 1 cut(s) 1285
BspACI CCGC 9 cut(s) 128, 807, 1656, 1865, 2000, 2130, 2229, 2473, 2507
BspANI GGCC 8 cut(s) 457, 886, 1805, 1818, 1828, 2015, 2115, 2287
BspCNI CTCAG 4 cut(s) 409, 647, 1051, 1654
BspEI TCCGGA 3 cut(s) 162, 1101, 2212
BspFNI CGCG 4 cut(s) 807, 1470, 1493, 2150
BspLI GGNNCC 6 cut(s) 1071, 1239, 1918, 2006, 2177, 2436
BspMAI CTGCAG 2 cut(s) 19, 969
BspMI ACCTGC 6 cut(s) 806, 863, 1036, 1509, 1592, 2328
BspPI GGATC 9 cut(s) 458, 575, 1380, 1538, 1641, 1673, 1832, 1896, 2420
BspQI GCTCTTC 2 cut(s) 254, 360
BspTNI GGTCTC 2 cut(s) 1526, 1694
BsrBI CCGCTC 2 cut(s) 2132, 2509
BsrDI GCAATG 1 cut(s) 135
BsrFI RCCGGY 1 cut(s) 218
BsrGI TGTACA 1 cut(s) 1285
BsrI ACTGG 7 cut(s) 790, 977, 1219, 1933, 2008, 2186, 2411
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 7 cut(s) 317, 438, 1359, 1782, 1800, 2171, 2430
BssT1I CCWWGG 3 cut(s) 438, 1782, 2171
Bst2UI CCWGG 6 cut(s) 503, 1254, 1361, 2117, 2432, 2439
Bst6I CTCTTC 4 cut(s) 254, 360, 510, 911
BstAPI GCANNNNNTGC 1 cut(s) 989
BstAUI TGTACA 1 cut(s) 1285
BstC8I GCNNGC 4 cut(s) 779, 1777, 2220, 2448
BstDEI CTNAG 4 cut(s) 417, 634, 1038, 1662
BstF5I GGATG 6 cut(s) 171, 1311, 1339, 1426, 2097, 2469
BstFNI CGCG 4 cut(s) 807, 1470, 1493, 2150
BstHHI GCGC 1 cut(s) 1470
BstMAI GTCTC 7 cut(s) 413, 1526, 1693, 1694, 1746, 1766, 1916
BstMWI GCNNNNNNNGC 7 cut(s) 188, 635, 964, 973, 989, 1465, 2238
BstNI CCWGG 6 cut(s) 503, 1254, 1361, 2117, 2432, 2439
BstNSI RCATGY 2 cut(s) 326, 1954
BstSFI CTRYAG 3 cut(s) 15, 965, 2236
BstSLI GKGCMC 3 cut(s) 376, 994, 2265
BstUI CGCG 4 cut(s) 807, 1470, 1493, 2150
BstV1I GCAGC 3 cut(s) 109, 112, 337
BstV2I GAAGAC 3 cut(s) 1186, 1650, 1787
BstX2I RGATCY 4 cut(s) 580, 1633, 1857, 1888
BstXI CCANNNNNNTGG 3 cut(s) 509, 1861, 2108
BstYI RGATCY 4 cut(s) 580, 1633, 1857, 1888
BsuRI GGCC 8 cut(s) 457, 886, 1805, 1818, 1828, 2015, 2115, 2287
BtgZI GCGATG 1 cut(s) 2088
BtrI CACGTC 1 cut(s) 2266
BtsCI GGATG 6 cut(s) 171, 1311, 1339, 1426, 2097, 2469
BtsIMutI CAGTG 5 cut(s) 783, 1350, 1487, 2015, 2404
BveI ACCTGC 6 cut(s) 806, 863, 1036, 1509, 1592, 2328
Cac8I GCNNGC 4 cut(s) 779, 1777, 2220, 2448
CfoI GCGC 1 cut(s) 1470
Cfr10I RCCGGY 1 cut(s) 218
Cfr9I CCCGGG 1 cut(s) 317
CseI GACGC 4 cut(s) 397, 1482, 1517, 2139
Csp6I GTAC 7 cut(s) 292, 1063, 1286, 1560, 1725, 1969, 2518
CspAI ACCGGT 1 cut(s) 218
CviQI GTAC 7 cut(s) 292, 1063, 1286, 1560, 1725, 1969, 2518
DdeI CTNAG 4 cut(s) 417, 634, 1038, 1662
DriI GACNNNNNGTC 2 cut(s) 334, 1929
EaeI YGGCCR 1 cut(s) 2113
Eam1104I CTCTTC 4 cut(s) 254, 360, 510, 911
Eam1105I GACNNNNNGTC 2 cut(s) 334, 1929
EarI CTCTTC 4 cut(s) 254, 360, 510, 911
EciI GGCGGA 2 cut(s) 1671, 1880
Ecl136II GAGCTC 1 cut(s) 499
Eco130I CCWWGG 3 cut(s) 438, 1782, 2171
Eco147I AGGCCT 1 cut(s) 457
Eco24I GRGCYC 1 cut(s) 501
Eco31I GGTCTC 2 cut(s) 1526, 1694
Eco47I GGWCC 6 cut(s) 1070, 1121, 1238, 1293, 1916, 1931
Eco53kI GAGCTC 1 cut(s) 499
Eco57I CTGAAG 2 cut(s) 360, 1137
Eco88I CYCGRG 3 cut(s) 317, 1799, 1919
EcoICRI GAGCTC 1 cut(s) 499
EcoO109I RGGNCCY 1 cut(s) 1121
EcoRII CCWGG 6 cut(s) 501, 1252, 1359, 2115, 2430, 2437
EcoT14I CCWWGG 3 cut(s) 438, 1782, 2171
EcoT38I GRGCYC 1 cut(s) 501
ErhI CCWWGG 3 cut(s) 438, 1782, 2171
Esp3I CGTCTC 3 cut(s) 1693, 1766, 1916
FalI AAGNNNNNCTT 4 cut(s) 570, 602, 1110, 1142
FaqI GGGAC 4 cut(s) 293, 1895, 1902, 2137
FauI CCCGC 3 cut(s) 121, 1993, 2514
FauNDI CATATG 1 cut(s) 574
FbaI TGATCA 1 cut(s) 2401
FblI GTMKAC 4 cut(s) 98, 606, 1211, 1947
Fnu4HI GCNGC 4 cut(s) 123, 126, 351, 2230
FokI GGATG 6 cut(s) 178, 1318, 1346, 1433, 2084, 2456
FriOI GRGCYC 1 cut(s) 501
Fsp4HI GCNGC 4 cut(s) 123, 126, 351, 2230
FspBI CTAG 1 cut(s) 2349
GlaI GCGC 1 cut(s) 1469
GluI GCNGC 4 cut(s) 123, 126, 351, 2230
GsaI CCCAGC 2 cut(s) 758, 2158
GsuI CTGGAG 2 cut(s) 485, 1275
HaeIII GGCC 8 cut(s) 457, 886, 1805, 1818, 1828, 2015, 2115, 2287
HapII CCGG 8 cut(s) 163, 219, 318, 611, 1102, 2213, 2289, 2373
HgaI GACGC 4 cut(s) 397, 1482, 1517, 2139
HhaI GCGC 1 cut(s) 1470
Hin6I GCGC 1 cut(s) 1468
HinP1I GCGC 1 cut(s) 1468
HincII GTYRAC 5 cut(s) 99, 328, 472, 706, 1948
HindII GTYRAC 5 cut(s) 99, 328, 472, 706, 1948
HindIII AAGCTT 2 cut(s) 252, 899
HpaI GTTAAC 1 cut(s) 472
HpaII CCGG 8 cut(s) 163, 219, 318, 611, 1102, 2213, 2289, 2373
Hpy188III TCNNGA 8 cut(s) 163, 412, 850, 1102, 1370, 1799, 1919, 2213
HpyCH4IV ACGT 1 cut(s) 2265
HpyF10VI GCNNNNNNNGC 7 cut(s) 188, 635, 964, 973, 989, 1465, 2238
HpyF3I CTNAG 4 cut(s) 417, 634, 1038, 1662
HpySE526I ACGT 1 cut(s) 2265
HspAI GCGC 1 cut(s) 1468
Kpn2I TCCGGA 3 cut(s) 162, 1101, 2212
Ksp22I TGATCA 1 cut(s) 2401
KspAI GTTAAC 1 cut(s) 472
LguI GCTCTTC 2 cut(s) 254, 360
LmnI GCTCC 7 cut(s) 432, 504, 1582, 1658, 2181, 2215, 2494
Lsp1109I GCAGC 3 cut(s) 109, 112, 337
LweI GCATC 4 cut(s) 1166, 1411, 2083, 2241
MaeI CTAG 1 cut(s) 2349
MaeII ACGT 1 cut(s) 2265
MaeIII GTNAC 2 cut(s) 343, 907
MbiI CCGCTC 2 cut(s) 2132, 2509
MflI RGATCY 4 cut(s) 580, 1633, 1857, 1888
MhlI GDGCHC 6 cut(s) 376, 429, 501, 994, 1587, 2265
MlsI TGGCCA 1 cut(s) 2115
MluCI AATT 5 cut(s) 33, 207, 932, 1243, 2277
MluI ACGCGT 1 cut(s) 2148
MluNI TGGCCA 1 cut(s) 2115
MlyI GAGTC 4 cut(s) 104, 305, 719, 1580
MmeI TCCRAC 2 cut(s) 978, 2150
Mox20I TGGCCA 1 cut(s) 2115
MroI TCCGGA 3 cut(s) 162, 1101, 2212
MroXI GAANNNNTTC 1 cut(s) 1794
MscI TGGCCA 1 cut(s) 2115
MseI TTAA 6 cut(s) 210, 471, 903, 1974, 2247, 2357
MslI CAYNNNNRTG 3 cut(s) 1304, 2091, 2106
Msp20I TGGCCA 1 cut(s) 2115
MspA1I CMGCKG 2 cut(s) 350, 638
MspI CCGG 8 cut(s) 163, 219, 318, 611, 1102, 2213, 2289, 2373
Mva1269I GAATGC 2 cut(s) 556, 1396
MvaI CCWGG 6 cut(s) 503, 1254, 1361, 2117, 2432, 2439
MvnI CGCG 4 cut(s) 807, 1470, 1493, 2150
MwoI GCNNNNNNNGC 7 cut(s) 188, 635, 964, 973, 989, 1465, 2238
NciI CCSGG 4 cut(s) 318, 319, 611, 2289
NdeI CATATG 1 cut(s) 574
NlaIV GGNNCC 6 cut(s) 1071, 1239, 1918, 2006, 2177, 2436
NmuCI GTSAC 2 cut(s) 343, 907
NspI RCATGY 2 cut(s) 326, 1954
OliI CACNNNNGTG 1 cut(s) 2106
PaqCI CACCTGC 2 cut(s) 1036, 1592
PceI AGGCCT 1 cut(s) 457
PciI ACATGT 1 cut(s) 1950
PciSI GCTCTTC 2 cut(s) 254, 360
PctI GAATGC 2 cut(s) 556, 1396
PdmI GAANNNNTTC 1 cut(s) 1794
PfeI GAWTC 7 cut(s) 1150, 1173, 1230, 1846, 1988, 2333, 2343
PflMI CCANNNNNTGG 2 cut(s) 590, 768
PfoI TCCNGGA 1 cut(s) 1252
PinAI ACCGGT 1 cut(s) 218
PkrI GCNGC 4 cut(s) 124, 127, 352, 2231
PleI GAGTC 4 cut(s) 104, 305, 718, 1579
PpsI GAGTC 4 cut(s) 104, 305, 718, 1579
PpuMI RGGWCCY 1 cut(s) 1121
PscI ACATGT 1 cut(s) 1950
PsiI TTATAA 1 cut(s) 1670
Psp124BI GAGCTC 1 cut(s) 501
Psp5II RGGWCCY 1 cut(s) 1121
Psp6I CCWGG 6 cut(s) 501, 1252, 1359, 2115, 2430, 2437
PspFI CCCAGC 2 cut(s) 754, 2154
PspGI CCWGG 6 cut(s) 501, 1252, 1359, 2115, 2430, 2437
PspN4I GGNNCC 6 cut(s) 1071, 1239, 1918, 2006, 2177, 2436
PspPPI RGGWCCY 1 cut(s) 1121
PstI CTGCAG 2 cut(s) 19, 969
PsuI RGATCY 4 cut(s) 580, 1633, 1857, 1888
PvuII CAGCTG 2 cut(s) 350, 638
RsaI GTAC 7 cut(s) 293, 1064, 1287, 1561, 1726, 1970, 2519
RsaNI GTAC 7 cut(s) 292, 1063, 1286, 1560, 1725, 1969, 2518
RseI CAYNNNNRTG 3 cut(s) 1304, 2091, 2106
SacI GAGCTC 1 cut(s) 501
SalI GTCGAC 2 cut(s) 97, 1946
SapI GCTCTTC 2 cut(s) 254, 360
SaqAI TTAA 6 cut(s) 210, 471, 903, 1974, 2247, 2357
SatI GCNGC 4 cut(s) 123, 126, 351, 2230
SchI GAGTC 4 cut(s) 104, 305, 719, 1580
SduI GDGCHC 6 cut(s) 376, 429, 501, 994, 1587, 2265
SfaNI GCATC 4 cut(s) 1166, 1411, 2083, 2241
SfcI CTRYAG 3 cut(s) 15, 965, 2236
SinI GGWCC 6 cut(s) 1070, 1121, 1238, 1293, 1916, 1931
SmaI CCCGGG 1 cut(s) 319
SmiMI CAYNNNNRTG 3 cut(s) 1304, 2091, 2106
SmlI CTYRAG 3 cut(s) 494, 1523, 1709
SmoI CTYRAG 3 cut(s) 494, 1523, 1709
Sse9I AATT 5 cut(s) 33, 207, 932, 1243, 2277
SseBI AGGCCT 1 cut(s) 457
SsiI CCGC 9 cut(s) 128, 807, 1656, 1865, 2000, 2130, 2229, 2473, 2507
SspMI CTAG 1 cut(s) 2349
SstI GAGCTC 1 cut(s) 501
StuI AGGCCT 1 cut(s) 457
StyI CCWWGG 3 cut(s) 438, 1782, 2171
TaiI ACGT 1 cut(s) 2268
TaqI TCGA 9 cut(s) 84, 98, 507, 1142, 1329, 1617, 1947, 2134, 2199
TasI AATT 5 cut(s) 33, 207, 932, 1243, 2277
TatI WGTACW 1 cut(s) 1285
TauI GCSGC 1 cut(s) 2232
TfiI GAWTC 7 cut(s) 1150, 1173, 1230, 1846, 1988, 2333, 2343
Tru1I TTAA 6 cut(s) 210, 471, 903, 1974, 2247, 2357
Tru9I TTAA 6 cut(s) 210, 471, 903, 1974, 2247, 2357
TscAI CASTG 5 cut(s) 790, 1357, 1487, 2015, 2411
TseFI GTSAC 2 cut(s) 343, 907
TseI GCWGC 3 cut(s) 122, 125, 350
Tsp45I GTSAC 2 cut(s) 343, 907
TspDTI ATGAA 9 cut(s) 264, 591, 843, 875, 929, 968, 1457, 1639, 1992
TspGWI ACGGA 5 cut(s) 17, 297, 419, 1685, 1981
TspMI CCCGGG 1 cut(s) 317
TspRI CASTG 5 cut(s) 790, 1357, 1487, 2015, 2411
Van91I CCANNNNNTGG 2 cut(s) 590, 768
VneI GTGCAC 1 cut(s) 372
VpaK11BI GGWCC 6 cut(s) 1070, 1121, 1238, 1293, 1916, 1931
XapI RAATTY 2 cut(s) 1243, 2277
XceI RCATGY 2 cut(s) 326, 1954
XcmI CCANNNNNNNNNTGG 2 cut(s) 562, 2108
XmaI CCCGGG 1 cut(s) 317
XmiI GTMKAC 4 cut(s) 98, 606, 1211, 1947
XmnI GAANNNNTTC 1 cut(s) 1794
XspI CTAG 1 cut(s) 2349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.