FvH4_3g15640

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0001995
Physical Location & Seq
Forward (+)
9831425 .. 9835720
4296 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g15640.t1

Sequence Viewer

Length: 2532 bp
ATGGTGAAGTTTACGGCAGAAGAGCTGCGTAGGATTATGGACTACAAGCATAACATTCGTAATATGTCTGTTATTGCCCATGTCGATCATGGTAAATCAACCCTTACAGACTCCCTTGTCGCTGCTGCCGGTATCATTGCACAAGAAGTTGCTGGTGATGTCCGGATGACTGATACCCGTGCTGATGAGGCAGAGCGTGGTATTACAATTAAATCTACTGGAATCTCTCTTTACTACGAGATGACTGACGAGTCTTTGAAGAGCTTCAAGGGAGAGAGGAACGGAAATGAGTACCTTATCAATCTCATCGATTCTCCCGGGCACGTTGACTTTTCATCTGAGGTTACTGCTGCTCTTCGTATTACTGATGGTGCCCTTGTGGTGGTGGATTGTATTGAGGGCGTGTGTGTCCAAACTGAGACCGTGCTCCGTCAAGCCTTGGGAGAAAGGATCAGGCCTGTTTTGACTGTTAACAAGATGGACAGGTGTTTCCTTGAGCTCCAGGTGGATGGCGAGGAGGCCTACCAGACATTCCAGAGGGTTATTGAGAATGCCAATGTCATCATGGCTACCTACGAAGACCCTCTTCTTGGTGATGTCCAGGTGTACCCCGAGAAAGGAACAGTTGCTTTCTCTGCTGGTTTGCACGGTTGGGCATTCACTCTCACCAACTTTGCCAAGATGTATGCATCTAAGTTTGGAGTTGATGAGTCAAAGATGATGGAAAGGCTCTGGGGTGAGAACTACTTTGATCCAGCTACCAAGAAGTGGACCACCAAAAGCACTGGTTCTGCTACCTGCAAGCGTGGTTTTGTCCAGTTTTGTTATGAACCAATCAAGCAGATTATCAACACCTGCATGAATGACCAGAAGGATAAGCTGTGGCCCATGTTGACAAAGCTCAATGTCACTATGAAGAGTGATGAAAAGGAACTTATGGGAAAGGCATTGATGAAGAGGGTCATGCAGAACTGGCTTCCGGCCAGCACTGCCCTATTGGAAATGATGATCTTTCACCTTCCCTCTCCACACACAGCTCAAAAGTACCGTGTTGAGAATTTGTACGAGGGTCCCCTGGATGACCAATATGCTAATGCTATCAGAAACTGTGATCCAGATGGTCCGCTTATGCTGTATGTATCTAAGATGATTCCGGCATCTGACAAGGGTAGATTCTTTGCTTTTGGTCGTGTGTTTGCTGGTAGGGTCCAAACTGGTTTGAAGGTTAGAATTATGGGACCAAACTATGTTCCTGGGGAAAAGAAGGATCTTTATGTCAAGAATGTACAGAGGACTGTTATCTGGATGGGAAAGAAACAAGAAACTGTTGAGGATGTTCCCTGTGGTAACACTGTTGCCTTGGTTGGTCTGGATCAGTTCATCACCAAGAATGCTACCTTGACAAATGAGAAGGAAACAGATGCTCACCCCATTCGTGCAATGAAGTTCTCTGTCTCACCTGTTGTGCGTGTTGCTGTTCAGTGTAAGGTTGCTTCTGATCTTCCCAAGCTTGTTGAAGGGCTGAAACGTCTGGCTAAGTCCGATCCTATGGTTGTCTGTACCATTGAGGAGTCTGGAGAGCACATCATTGCTGGAGCTGGTGAACTTCACCTTGAGATCTGCTTGAAGGATCTGCAAGATGATTTTATGGGTGGAGCGGAAATTGTAAAATCTGATCCTGTTGTGTCCTTCCGTGAGACAGTCCTTGAGAAGTCCTGCCGTACTGTGATGAGCAAGTCTCCCAACAAGCACAACCGTCTGTACATGGAAGCACGCCCGTTGGAGGAAGGTCTTCCTGAGGCCATTGATGATGGTCGTATTGGTCCAAGGGATGATCCTAAAATCCGCTCAAAGATCTTGGCTGAGGAATTCGGTTGGGACAAGGATCTTGCTAAGAAAATCTGGTGCTTCGGTCCTGAGACCACTGGTCCTAACATGGTGGTTGATATGTGTAAGGGAGTCCAGTACCTCAATGAAATCAAGGATTCAGTTGTTGCTGGGTTCCAGTGGGCTTCAAAGGAAGGTGCGTTGGCTGAAGAAAACATGAGGGGTATCTGCTTTGAAGTTTGTGATGTTGTTCTCCATGCTGATGCTATTCACAGAGGAGGTGGTCAGGTCATTCCAACCGCCAGGAGGGTTATCTATGCTTCTCAGCTTACAGCGAAGCCAAGGCTCCTTGAACCTGTGTATCTTGTTGAGATTCAAGCTCCTGAGGGTGCTCTTGGTGGTATCTACAGTGTTCTCAATCAGAAGCGTGGACACGTCTTTGAAGAAATGCAGAGGCCTGGCACTCCACTCTACAACATCAAGGCCTACCTCCCTGTCATCGAGTCTTTCGGTTTCTCTGGTCAACTGAGGGCTGCAACTTCTGGCCAGGCTTTCCCCCAGTGTGTGTTCGATCATTGGGAGATGATGTCTTCTGATCCACTGGAAGCTGGTTCCCAGGCAGCTCAGCTTGTTCAAGACATCCGCAAGAGGAAGGGCCTGAAGGAGCAAATGACCCCGCTATCTGAGTTTGAGGACAAGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04152 ko04921 map04152 map04921
Pfam Domains
Protein Families
EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

844

Amino Acids

94.16

Weight (kDa)

5.84

Isoelectric Point (pI)

34.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 18 - 227 1.4e-48 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 379 - 469 4.5e-06 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 394 - 462 2.5e-09 Elongation factor Tu domain 2
EFG_III PF14492 487 - 549 8.5e-12 Elongation Factor G, domain III
EFG_IV PF03764 610 - 722 2e-29 Elongation factor G, domain IV
EFG_C PF00679 724 - 811 1.4e-20 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 863
AasI GACNNNNNNGTC 2 cut(s) 116, 250
Acc36I ACCTGC 2 cut(s) 806, 863
AccB1I GGYRCC 1 cut(s) 371
AccB7I CCANNNNNTGG 1 cut(s) 768
AccBSI CCGCTC 2 cut(s) 1658, 1848
AccIII TCCGGA 1 cut(s) 162
AciI CCGC 6 cut(s) 1124, 1658, 1846, 2127, 2470, 2504
AcoI YGGCCR 2 cut(s) 981, 2371
AcsI RAATTY 2 cut(s) 1057, 1868
AcuI CTGAAG 2 cut(s) 2055, 2507
AfaI GTAC 9 cut(s) 293, 608, 1046, 1064, 1287, 1561, 1723, 1763, 1967
AfiI CCNNNNNNNGG 6 cut(s) 382, 590, 617, 768, 1783, 2133
AflIII ACRYGT 1 cut(s) 2260
AhdI GACNNNNNGTC 1 cut(s) 1926
AjiI CACGTC 1 cut(s) 2263
AjnI CCWGG 8 cut(s) 501, 600, 1074, 1252, 2129, 2284, 2373, 2442
Alw21I GWGCWC 4 cut(s) 429, 501, 1584, 2221
Alw26I GTCTC 5 cut(s) 413, 1459, 1691, 1743, 1913
AlwNI CAGNNNCTG 1 cut(s) 1107
Ama87I CYCGRG 2 cut(s) 317, 611
Aor13HI TCCGGA 1 cut(s) 162
AoxI GGCC 9 cut(s) 455, 519, 884, 981, 1800, 2282, 2310, 2371, 2482
ApeKI GCWGC 6 cut(s) 25, 122, 125, 350, 2360, 2447
ApoI RAATTY 2 cut(s) 1057, 1868
Asp700I GAANNNNTTC 2 cut(s) 263, 1791
AsuC2I CCSGG 2 cut(s) 318, 319
AvaI CYCGRG 2 cut(s) 317, 611
AvaII GGWCC 8 cut(s) 771, 1070, 1121, 1207, 1238, 1823, 1913, 1928
AxyI CCTNAGG 2 cut(s) 1797, 2211
BaeGI GKGCMC 2 cut(s) 324, 376
BaeI ACNNNNGTAYC 2 cut(s) 2171, 2204
BalI TGGCCA 1 cut(s) 2373
BanI GGYRCC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 501
BarI GAAGNNNNNNTAC 2 cut(s) 1477, 1509
BbsI GAAGAC 3 cut(s) 585, 1784, 2409
Bbv12I GWGCWC 4 cut(s) 429, 501, 1584, 2221
BbvCI CCTCAGC 1 cut(s) 1863
BbvI GCAGC 6 cut(s) 12, 109, 112, 337, 2347, 2459
BccI CCATC 7 cut(s) 362, 472, 503, 715, 1112, 1300, 1805
BceAI ACGGC 2 cut(s) 30, 1704
BcgI CGANNNNNNTGC 2 cut(s) 38, 72
BciT130I CCWGG 8 cut(s) 503, 602, 1076, 1254, 2131, 2286, 2375, 2444
BcnI CCSGG 2 cut(s) 318, 319
BcoDI GTCTC 5 cut(s) 413, 1459, 1691, 1743, 1913
BfmI CTRYAG 1 cut(s) 2233
BfuAI ACCTGC 2 cut(s) 806, 863
BglII AGATCT 2 cut(s) 1617, 1854
BisI GCNGC 6 cut(s) 26, 123, 126, 351, 2361, 2448
BlpI GCTNAGC 1 cut(s) 2451
BlsI GCNGC 6 cut(s) 27, 124, 127, 352, 2362, 2449
Bme18I GGWCC 8 cut(s) 771, 1070, 1121, 1207, 1238, 1823, 1913, 1928
BmeRI GACNNNNNGTC 1 cut(s) 1926
BmeT110I CYCGRG 2 cut(s) 317, 611
BmgBI CACGTC 1 cut(s) 2263
BmiI GGNNCC 8 cut(s) 373, 1071, 1072, 1208, 1239, 2003, 2174, 2440
BmrI ACTGGG 1 cut(s) 2380
BmsI GCATC 4 cut(s) 698, 1166, 1411, 2080
BmuI ACTGGG 1 cut(s) 2380
BpiI GAAGAC 3 cut(s) 585, 1784, 2409
BplI GAGNNNNNCTC 2 cut(s) 1723, 1755
BpmI CTGGAG 3 cut(s) 485, 1596, 1614
Bpu10I CCTNAGC 1 cut(s) 1863
Bpu1102I GCTNAGC 1 cut(s) 2451
BpuEI CTTGAG 3 cut(s) 515, 1634, 1727
BpuMI CCSGG 2 cut(s) 318, 319
Bsa29I ATCGAT 1 cut(s) 309
BsaI GGTCTC 2 cut(s) 413, 1913
BsaJI CCNNGG 8 cut(s) 317, 438, 1074, 1253, 1359, 1826, 2168, 2442
BsaWI WCCGGW 1 cut(s) 162
BsaXI ACNNNNNCTCC 2 cut(s) 2399, 2429
Bsc4I CCNNNNNNNGG 6 cut(s) 382, 590, 617, 768, 1783, 2133
Bse118I RCCGGY 1 cut(s) 128
Bse21I CCTNAGG 2 cut(s) 1797, 2211
Bse3DI GCAATG 3 cut(s) 135, 1446, 1587
BseAI TCCGGA 1 cut(s) 162
BseBI CCWGG 8 cut(s) 503, 602, 1076, 1254, 2131, 2286, 2375, 2444
BseCI ATCGAT 1 cut(s) 309
BseDI CCNNGG 8 cut(s) 317, 438, 1074, 1253, 1359, 1826, 2168, 2442
BseGI GGATG 7 cut(s) 171, 514, 1084, 1311, 1339, 1837, 2466
BseLI CCNNNNNNNGG 6 cut(s) 382, 590, 617, 768, 1783, 2133
BseMI GCAATG 3 cut(s) 135, 1446, 1587
BseRI GAGGAG 3 cut(s) 530, 1583, 2118
BseSI GKGCMC 2 cut(s) 324, 376
BseXI GCAGC 6 cut(s) 12, 109, 112, 337, 2347, 2459
BseYI CCCAGC 1 cut(s) 1997
BshFI GGCC 9 cut(s) 457, 521, 886, 983, 1802, 2284, 2312, 2373, 2484
BshNI GGYRCC 1 cut(s) 371
BshVI ATCGAT 1 cut(s) 309
BsiHKAI GWGCWC 4 cut(s) 429, 501, 1584, 2221
BsiHKCI CYCGRG 2 cut(s) 317, 611
BsiSI CCGG 5 cut(s) 129, 163, 318, 980, 1154
BslFI GGGAC 3 cut(s) 1056, 1251, 1892
BslI CCNNNNNNNGG 6 cut(s) 382, 590, 617, 768, 1783, 2133
BsmAI GTCTC 5 cut(s) 413, 1459, 1691, 1743, 1913
BsmFI GGGAC 3 cut(s) 1056, 1251, 1892
BsmI GAATGC 3 cut(s) 556, 656, 1396
BsnI GGCC 9 cut(s) 457, 521, 886, 983, 1802, 2284, 2312, 2373, 2484
Bso31I GGTCTC 2 cut(s) 413, 1913
BsoBI CYCGRG 2 cut(s) 317, 611
Bsp1286I GDGCHC 6 cut(s) 324, 376, 429, 501, 1584, 2221
Bsp13I TCCGGA 1 cut(s) 162
Bsp1407I TGTACA 2 cut(s) 1285, 1761
Bsp1720I GCTNAGC 1 cut(s) 2451
BspACI CCGC 6 cut(s) 1124, 1658, 1846, 2127, 2470, 2504
BspANI GGCC 9 cut(s) 457, 521, 886, 983, 1802, 2284, 2312, 2373, 2484
BspDI ATCGAT 1 cut(s) 309
BspEI TCCGGA 1 cut(s) 162
BspLI GGNNCC 8 cut(s) 373, 1071, 1072, 1208, 1239, 2003, 2174, 2440
BspMI ACCTGC 2 cut(s) 806, 863
BspQI GCTCTTC 3 cut(s) 15, 254, 360
BspT107I GGYRCC 1 cut(s) 371
BspTNI GGTCTC 2 cut(s) 413, 1913
BsrBI CCGCTC 2 cut(s) 1658, 1848
BsrDI GCAATG 3 cut(s) 135, 1446, 1587
BsrFI RCCGGY 1 cut(s) 128
BsrGI TGTACA 2 cut(s) 1285, 1761
BssAI RCCGGY 1 cut(s) 128
BssECI CCNNGG 8 cut(s) 317, 438, 1074, 1253, 1359, 1826, 2168, 2442
BssT1I CCWWGG 4 cut(s) 438, 1359, 1826, 2168
Bst2UI CCWGG 8 cut(s) 503, 602, 1076, 1254, 2131, 2286, 2375, 2444
Bst6I CTCTTC 6 cut(s) 15, 254, 360, 591, 911, 950
BstAUI TGTACA 2 cut(s) 1285, 1761
BstC8I GCNNGC 3 cut(s) 803, 985, 1774
BstF5I GGATG 7 cut(s) 171, 514, 1084, 1311, 1339, 1837, 2466
BstMAI GTCTC 5 cut(s) 413, 1459, 1691, 1743, 1913
BstMWI GCNNNNNNNGC 4 cut(s) 188, 635, 973, 989
BstNI CCWGG 8 cut(s) 503, 602, 1076, 1254, 2131, 2286, 2375, 2444
BstSFI CTRYAG 1 cut(s) 2233
BstSLI GKGCMC 2 cut(s) 324, 376
BstV1I GCAGC 6 cut(s) 12, 109, 112, 337, 2347, 2459
BstV2I GAAGAC 3 cut(s) 585, 1784, 2409
BstX2I RGATCY 5 cut(s) 1267, 1617, 1630, 1854, 1885
BstXI CCANNNNNNTGG 1 cut(s) 509
BstYI RGATCY 5 cut(s) 1267, 1617, 1630, 1854, 1885
Bsu15I ATCGAT 1 cut(s) 309
Bsu36I CCTNAGG 2 cut(s) 1797, 2211
BsuRI GGCC 9 cut(s) 457, 521, 886, 983, 1802, 2284, 2312, 2373, 2484
BsuTUI ATCGAT 1 cut(s) 309
BtrI CACGTC 1 cut(s) 2263
BtsCI GGATG 7 cut(s) 171, 514, 1084, 1311, 1339, 1837, 2466
BtsI GCAGTG 1 cut(s) 987
BtsIMutI CAGTG 9 cut(s) 783, 987, 1350, 1487, 1923, 2012, 2242, 2393, 2426
BveI ACCTGC 2 cut(s) 806, 863
Cac8I GCNNGC 3 cut(s) 803, 985, 1774
CaiI CAGNNNCTG 1 cut(s) 1107
Cfr10I RCCGGY 1 cut(s) 128
Cfr9I CCCGGG 1 cut(s) 317
ClaI ATCGAT 1 cut(s) 309
Csp6I GTAC 9 cut(s) 292, 607, 1045, 1063, 1286, 1560, 1722, 1762, 1966
CviQI GTAC 9 cut(s) 292, 607, 1045, 1063, 1286, 1560, 1722, 1762, 1966
DrdI GACNNNNNNGTC 2 cut(s) 116, 250
DriI GACNNNNNGTC 1 cut(s) 1926
DseDI GACNNNNNNGTC 2 cut(s) 116, 250
EaeI YGGCCR 2 cut(s) 981, 2371
Eam1104I CTCTTC 6 cut(s) 15, 254, 360, 591, 911, 950
Eam1105I GACNNNNNGTC 1 cut(s) 1926
EarI CTCTTC 6 cut(s) 15, 254, 360, 591, 911, 950
Ecl136II GAGCTC 1 cut(s) 499
Eco130I CCWWGG 4 cut(s) 438, 1359, 1826, 2168
Eco147I AGGCCT 4 cut(s) 457, 521, 2284, 2312
Eco24I GRGCYC 1 cut(s) 501
Eco31I GGTCTC 2 cut(s) 413, 1913
Eco47I GGWCC 8 cut(s) 771, 1070, 1121, 1207, 1238, 1823, 1913, 1928
Eco53kI GAGCTC 1 cut(s) 499
Eco57I CTGAAG 2 cut(s) 2055, 2507
Eco81I CCTNAGG 2 cut(s) 1797, 2211
Eco88I CYCGRG 2 cut(s) 317, 611
EcoICRI GAGCTC 1 cut(s) 499
EcoO109I RGGNCCY 2 cut(s) 1070, 2482
EcoRI GAATTC 1 cut(s) 1868
EcoRII CCWGG 8 cut(s) 501, 600, 1074, 1252, 2129, 2284, 2373, 2442
EcoT14I CCWWGG 4 cut(s) 438, 1359, 1826, 2168
EcoT22I ATGCAT 1 cut(s) 691
EcoT38I GRGCYC 1 cut(s) 501
ErhI CCWWGG 4 cut(s) 438, 1359, 1826, 2168
FalI AAGNNNNNCTT 2 cut(s) 570, 602
FaqI GGGAC 3 cut(s) 1056, 1251, 1892
FauI CCCGC 1 cut(s) 2511
Fnu4HI GCNGC 6 cut(s) 26, 123, 126, 351, 2361, 2448
FokI GGATG 7 cut(s) 178, 521, 1091, 1318, 1346, 1844, 2453
FriOI GRGCYC 1 cut(s) 501
Fsp4HI GCNGC 6 cut(s) 26, 123, 126, 351, 2361, 2448
GluI GCNGC 6 cut(s) 26, 123, 126, 351, 2361, 2448
GsaI CCCAGC 1 cut(s) 2001
GsuI CTGGAG 3 cut(s) 485, 1596, 1614
HaeIII GGCC 9 cut(s) 457, 521, 886, 983, 1802, 2284, 2312, 2373, 2484
HapII CCGG 5 cut(s) 129, 163, 318, 980, 1154
HincII GTYRAC 4 cut(s) 328, 472, 894, 2351
HindII GTYRAC 4 cut(s) 328, 472, 894, 2351
HindIII AAGCTT 1 cut(s) 1508
HpaI GTTAAC 1 cut(s) 472
HpaII CCGG 5 cut(s) 129, 163, 318, 980, 1154
Hpy166II GTNNAC 9 cut(s) 12, 328, 472, 607, 771, 894, 1604, 2258, 2351
Hpy188I TCNGA 9 cut(s) 340, 1103, 1162, 1498, 1543, 1675, 2250, 2422, 2512
Hpy8I GTNNAC 9 cut(s) 12, 328, 472, 607, 771, 894, 1604, 2258, 2351
HpyCH4IV ACGT 3 cut(s) 324, 1528, 2262
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 635, 973, 989
HpySE526I ACGT 3 cut(s) 324, 1528, 2262
KflI GGGWCCC 1 cut(s) 1070
Kpn2I TCCGGA 1 cut(s) 162
KspAI GTTAAC 1 cut(s) 472
LguI GCTCTTC 3 cut(s) 15, 254, 360
LmnI GCTCC 7 cut(s) 432, 504, 1595, 1655, 2178, 2212, 2491
Lsp1109I GCAGC 6 cut(s) 12, 109, 112, 337, 2347, 2459
LweI GCATC 4 cut(s) 698, 1166, 1411, 2080
MaeII ACGT 3 cut(s) 324, 1528, 2262
MaeIII GTNAC 3 cut(s) 343, 907, 1346
MbiI CCGCTC 2 cut(s) 1658, 1848
MflI RGATCY 5 cut(s) 1267, 1617, 1630, 1854, 1885
MhlI GDGCHC 6 cut(s) 324, 376, 429, 501, 1584, 2221
MlsI TGGCCA 1 cut(s) 2373
MluCI AATT 5 cut(s) 207, 1057, 1230, 1662, 1868
MluNI TGGCCA 1 cut(s) 2373
MlyI GAGTC 6 cut(s) 104, 260, 719, 1580, 1968, 2339
MmeI TCCRAC 2 cut(s) 1761, 2147
Mox20I TGGCCA 1 cut(s) 2373
Mph1103I ATGCAT 1 cut(s) 691
MroI TCCGGA 1 cut(s) 162
MroXI GAANNNNTTC 2 cut(s) 263, 1791
MscI TGGCCA 1 cut(s) 2373
MseI TTAA 2 cut(s) 210, 471
MslI CAYNNNNRTG 2 cut(s) 857, 2088
Msp20I TGGCCA 1 cut(s) 2373
MspI CCGG 5 cut(s) 129, 163, 318, 980, 1154
Mva1269I GAATGC 3 cut(s) 556, 656, 1396
MvaI CCWGG 8 cut(s) 503, 602, 1076, 1254, 2131, 2286, 2375, 2444
MwoI GCNNNNNNNGC 4 cut(s) 188, 635, 973, 989
NciI CCSGG 2 cut(s) 318, 319
NlaIV GGNNCC 8 cut(s) 373, 1071, 1072, 1208, 1239, 2003, 2174, 2440
NmuCI GTSAC 1 cut(s) 907
NsiI ATGCAT 1 cut(s) 691
PaqCI CACCTGC 1 cut(s) 863
PceI AGGCCT 4 cut(s) 457, 521, 2284, 2312
PciSI GCTCTTC 3 cut(s) 15, 254, 360
PctI GAATGC 3 cut(s) 556, 656, 1396
PdmI GAANNNNTTC 2 cut(s) 263, 1791
PfeI GAWTC 6 cut(s) 222, 311, 1150, 1173, 1985, 2200
PflMI CCANNNNNTGG 1 cut(s) 768
PkrI GCNGC 6 cut(s) 27, 124, 127, 352, 2362, 2449
PleI GAGTC 6 cut(s) 104, 259, 718, 1579, 1967, 2338
PpsI GAGTC 6 cut(s) 104, 259, 718, 1579, 1967, 2338
PpuMI RGGWCCY 1 cut(s) 1070
Psp124BI GAGCTC 1 cut(s) 501
Psp5II RGGWCCY 1 cut(s) 1070
Psp6I CCWGG 8 cut(s) 501, 600, 1074, 1252, 2129, 2284, 2373, 2442
PspFI CCCAGC 1 cut(s) 1997
PspGI CCWGG 8 cut(s) 501, 600, 1074, 1252, 2129, 2284, 2373, 2442
PspN4I GGNNCC 8 cut(s) 373, 1071, 1072, 1208, 1239, 2003, 2174, 2440
PspPPI RGGWCCY 1 cut(s) 1070
PstNI CAGNNNCTG 1 cut(s) 1107
PsuI RGATCY 5 cut(s) 1267, 1617, 1630, 1854, 1885
RsaI GTAC 9 cut(s) 293, 608, 1046, 1064, 1287, 1561, 1723, 1763, 1967
RsaNI GTAC 9 cut(s) 292, 607, 1045, 1063, 1286, 1560, 1722, 1762, 1966
RseI CAYNNNNRTG 2 cut(s) 857, 2088
SacI GAGCTC 1 cut(s) 501
SapI GCTCTTC 3 cut(s) 15, 254, 360
SaqAI TTAA 2 cut(s) 210, 471
SatI GCNGC 6 cut(s) 26, 123, 126, 351, 2361, 2448
SchI GAGTC 6 cut(s) 104, 260, 719, 1580, 1968, 2339
SduI GDGCHC 6 cut(s) 324, 376, 429, 501, 1584, 2221
SfaNI GCATC 4 cut(s) 698, 1166, 1411, 2080
SfcI CTRYAG 1 cut(s) 2233
SinI GGWCC 8 cut(s) 771, 1070, 1121, 1207, 1238, 1823, 1913, 1928
SmaI CCCGGG 1 cut(s) 319
SmiMI CAYNNNNRTG 2 cut(s) 857, 2088
SmlI CTYRAG 3 cut(s) 494, 1613, 1706
SmoI CTYRAG 3 cut(s) 494, 1613, 1706
Sse9I AATT 5 cut(s) 207, 1057, 1230, 1662, 1868
SseBI AGGCCT 4 cut(s) 457, 521, 2284, 2312
SsiI CCGC 6 cut(s) 1124, 1658, 1846, 2127, 2470, 2504
SstI GAGCTC 1 cut(s) 501
StuI AGGCCT 4 cut(s) 457, 521, 2284, 2312
StyI CCWWGG 4 cut(s) 438, 1359, 1826, 2168
TaiI ACGT 3 cut(s) 327, 1531, 2265
TaqI TCGA 4 cut(s) 84, 309, 2328, 2397
TaqII GACCGA 1 cut(s) 1901
TasI AATT 5 cut(s) 207, 1057, 1230, 1662, 1868
TatI WGTACW 2 cut(s) 1285, 1761
TfiI GAWTC 6 cut(s) 222, 311, 1150, 1173, 1985, 2200
Tru1I TTAA 2 cut(s) 210, 471
Tru9I TTAA 2 cut(s) 210, 471
TscAI CASTG 9 cut(s) 790, 994, 1357, 1487, 1930, 2012, 2242, 2393, 2433
TseFI GTSAC 1 cut(s) 907
TseI GCWGC 6 cut(s) 25, 122, 125, 350, 2360, 2447
Tsp45I GTSAC 1 cut(s) 907
TspDTI ATGAA 9 cut(s) 324, 843, 875, 929, 939, 968, 1369, 1457, 1989
TspGWI ACGGA 3 cut(s) 297, 419, 1682
TspMI CCCGGG 1 cut(s) 317
TspRI CASTG 9 cut(s) 790, 994, 1357, 1487, 1930, 2012, 2242, 2393, 2433
Van91I CCANNNNNTGG 1 cut(s) 768
VpaK11BI GGWCC 8 cut(s) 771, 1070, 1121, 1207, 1238, 1823, 1913, 1928
XapI RAATTY 2 cut(s) 1057, 1868
XcmI CCANNNNNNNNNTGG 2 cut(s) 86, 562
XmaI CCCGGG 1 cut(s) 317
XmnI GAANNNNTTC 2 cut(s) 263, 1791
Zsp2I ATGCAT 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.