Rh5AG184900

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001995
Physical Location & Seq
Forward (+)
21132993 .. 21137239
4247 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG184900.1

Sequence Viewer

Length: 2496 bp
ATGGACTACAAGCACAACATCCGTAATATGTCCGTTATTGCCCATGTCGATCATGGTAAATCTACCCTTACGGACTCCCTTGTCGCTGCTGCTGGTATCATTGCACAAGAAACTGCTGGTGATGTCCGGATGACGGATACCCGTGCAGATGAGGCAGAGCGTGGTATCACAATTAAGTCAACTGGAATCTCTCTCTACTATGAGATGACGGATGAGTCTTTGGCAAGCTTCAAGGGAGAGAGGAATGGAAATGAGTACCTTATCAATCTCATTGATTCCCCTGGGCATGTTGACTTTTCATCTGAGGTAACTGCTGCTCTTCGTATTACTGATGGTGCACTTGTGGTGGTGGATTGTATTGAGGGCGTGTGTGTCCAAACTGAGACTGTGCTCCGTCAAGCCTTGGGAGAAAGGATCAGGCCTGTGTTGACTGTTAACAAGATGGACAGGTGCTTCCTTGAACTCCAGGTGGATGGTGAAGAGGCCTACCAGAATTTTCAGAGGGTTATTGAGAATGCTAATGTCATCATGGCTACCTATGAAGACCCTCTTCTTGGTGATGTCCAGGTCTACCCTGAGAAAGGAACAGTTGCTTTCTCTGCTGGTTTGCACGGTTGGGCTTTTACTTTAACCAACTTTGCCAAGATGTATGCTGAAAAGTTTAAAGTTGATGAGGCAAAGATGATGGAAAGGCTTTGGGGTGAGAACTTTTTTGACCCAGCAACCAAGAAGTGGACCAGCAAGAACACCGGTTCTGCTACCTGCAAGCGTGGTTTCGTTCAGTTCTGTTATGAACCCATCAAGCAGGTTATCGCTACCTGCATGAATGACCAGAAGGATAAGCTGTGGCCCATGTTGGCAAAGCTTGGAATCACCATGAAGGGTGACGAGAAAGATCTGATGGGAAAGCCATTGATGAAGAGGGTCATGCAGACCTGGCTGCCAGCCAGCACTGCCCTATTGGAGATGATGATCTTTCACCTTCCCTCTCCACATACAGCTCAGAGGTATCGTGTGGAGAATCTTTACGAGGGTCCCCTGGATGATCAGTATGCTAATGCTATCAGAAACTGCGATCCTGATGGTCCTCTTATGCTCTATGTATCTAAGATGATTCCTGCATCTGACAAGGGTAGGTTCTTTGCCTTTGGCCGTGTGTTTGCTGGGAGGGTCCAAACAGGTTTGAAGGTTAGAATCATGGGACCAAACTATGTTCCCGGGGAAAAGAAGGATCTGTATGTGAAGAATGTACAGAGGACTGTTATCTGGATGGGAAAGAAACAAGAAACTGTTGAGGATGTTCCCTGTGGTAACACTGTTGCCTTGGTTGGTCTGGATCAGTTCATCACCAAGAATGCTACCTTGACAAATGAGAAGGAATCTGATGCTCACCCCATTCGTGCTATGAAGTTCTCTGTCTCTCCTGTTGTGCGTGTTGCTGTTCAGTGCAAGGTTGCTTCAGATCTTCCCAAGCTTGTTGAAGGTCTCAAGCGTCTGGCAAAGTCTGATCCTATGGTTGTCTGTTCTATTGAGGAGTCTGGAGAGCACATTATTGCCGGTGCTGGTGAACTCCACCTTGAGATCTGTTTGAAGGATCTTCAGGATGATTTTATGGGTGGAGCTGAAATTGTAAAATCTGACCCTGTTGTGTCCTTCCGTGAGACTGTCCTTGAGAAGTCTGTCCGTACAGTGATGAGCAAGTCCCCCAACAAGCATAACCGTTTGTACATGGAAGCACGCCCATTGGAAGAAGGTCTTGCAGAGGCCATTGATGATGGCCGTATTGGTCCAAGGGATGATCCTAAAATTCGTTCCAAGATCTTGGCCGAGGAATTTGGTTGGGACAAGGATCTTGCTAAGAAAATCTGGTGTTTTGGCCCTGAGACCACTGGTCCTAACATGGTGGTTGACATGTGTAAGGGAGTTCAGTACCTTAATGAAATCAAGGACTCTGTTGTTGCTGGGTTCCAGTGGGCTTCAAAGGAAGGTGCATTGGCTGAAGAAAACATGAGGGGTATTTGCTTTGAAGTCTGTGATGTTGTTCTTCATGCTGATGCTATTCACAGAGGAGGTGGTCAGGTCATTCCAACCGCCAGGAGGGTTATCTATGCTTCTCAGCTTACAGCCAAGCCAAGGCTCCTTGAACCTGTATATCTTGTTGAAATTCAAGCCCCTGAGGGTGCTCTTGGAGGTATCTACAGTGTTCTCAATCAGAAGCGTGGACATGTTTTCGAAGAAATTCAGAGGCCTGGTACCCCACTGTACAACATCAAGGCATACCTGCCTGTTGTTGAATCTTTTGGGTTCTCTGGTCAGTTGAGGGCTTCCACTTCAGGACAGGCTTTCCCCCAGTGTGTGTTTGATCATTGGGAGATGATGTCTTCTGATCCGTTGGAGGCTGGTTCCCAGGCATCTCAGCTGGTTCAAGACATCCGTAAGAGGAAGGGTTTGAAGGAGCAAATGACCCCGCTTTCTGACTTTGAGGACAAGCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04152 ko04921 map04152 map04921
Pfam Domains
Protein Families
EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

831

Amino Acids

92.54

Weight (kDa)

5.71

Isoelectric Point (pI)

30.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 6 - 215 6.2e-49 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 367 - 457 4.4e-06 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 382 - 450 2.4e-09 Elongation factor Tu domain 2
EFG_III PF14492 475 - 537 9e-12 Elongation Factor G, domain III
EFG_IV PF03764 598 - 710 2e-29 Elongation factor G, domain IV
EFG_C PF00679 712 - 799 5.5e-20 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 80, 214
Acc36I ACCTGC 4 cut(s) 770, 796, 827, 2289
Acc65I GGTACC 1 cut(s) 2252
AccB1I GGYRCC 1 cut(s) 2252
AccB7I CCANNNNNTGG 1 cut(s) 732
AccI GTMKAC 1 cut(s) 570
AccIII TCCGGA 1 cut(s) 126
AciI CCGC 2 cut(s) 2091, 2468
AclWI GGATC 9 cut(s) 422, 1070, 1239, 1344, 1502, 1602, 1793, 1857, 2381
AcoI YGGCCR 3 cut(s) 1150, 1777, 1824
AcsI RAATTY 5 cut(s) 493, 1806, 1832, 2163, 2238
AcuI CTGAAG 4 cut(s) 1443, 1583, 2019, 2316
AfaI GTAC 7 cut(s) 257, 1251, 1687, 1727, 1931, 2254, 2264
AfiI CCNNNNNNNGG 6 cut(s) 133, 554, 581, 732, 2097, 2133
AflIII ACRYGT 2 cut(s) 1911, 2224
AgeI ACCGGT 1 cut(s) 749
AhdI GACNNNNNGTC 1 cut(s) 1890
AjnI CCWGG 8 cut(s) 280, 465, 564, 935, 1038, 2093, 2248, 2406
AluBI AGCT 9 cut(s) 228, 844, 865, 1001, 1474, 1622, 2119, 2419, 2491
AluI AGCT 9 cut(s) 228, 844, 865, 1001, 1474, 1622, 2119, 2419, 2491
Alw21I GWGCWC 4 cut(s) 340, 393, 1548, 2185
Alw26I GTCTC 5 cut(s) 377, 1423, 1490, 1655, 1877
Alw44I GTGCAC 1 cut(s) 336
AlwI GGATC 9 cut(s) 422, 1070, 1239, 1344, 1502, 1602, 1793, 1857, 2381
AlwNI CAGNNNCTG 1 cut(s) 1071
Ama87I CYCGRG 1 cut(s) 1217
Aor13HI TCCGGA 1 cut(s) 126
AoxI GGCC 9 cut(s) 419, 483, 848, 1150, 1764, 1777, 1824, 1876, 2246
ApaLI GTGCAC 1 cut(s) 336
ApeKI GCWGC 4 cut(s) 86, 89, 314, 940
ApoI RAATTY 5 cut(s) 493, 1806, 1832, 2163, 2238
AsiGI ACCGGT 1 cut(s) 749
Asp700I GAANNNNTTC 1 cut(s) 2238
Asp718I GGTACC 1 cut(s) 2252
AspS9I GGNCC 9 cut(s) 735, 849, 1034, 1085, 1171, 1202, 1787, 1877, 1892
AsuC2I CCSGG 2 cut(s) 1218, 1219
AsuII TTCGAA 1 cut(s) 2232
AvaI CYCGRG 1 cut(s) 1217
AvaII GGWCC 7 cut(s) 735, 1034, 1085, 1171, 1202, 1787, 1892
AxyI CCTNAGG 1 cut(s) 2175
BaeGI GKGCMC 1 cut(s) 340
BanI GGYRCC 1 cut(s) 2252
BbsI GAAGAC 2 cut(s) 549, 2373
Bbv12I GWGCWC 4 cut(s) 340, 393, 1548, 2185
BbvI GCAGC 4 cut(s) 73, 76, 301, 927
BccI CCATC 9 cut(s) 326, 436, 467, 679, 806, 895, 1076, 1264, 1769
BceAI ACGGC 2 cut(s) 1137, 1764
BciT130I CCWGG 8 cut(s) 282, 467, 566, 937, 1040, 2095, 2250, 2408
BciVI GTATCC 1 cut(s) 130
BclI TGATCA 2 cut(s) 1045, 2362
BcnI CCSGG 2 cut(s) 1218, 1219
BcoDI GTCTC 5 cut(s) 377, 1423, 1490, 1655, 1877
BfmI CTRYAG 1 cut(s) 2197
BfuAI ACCTGC 4 cut(s) 770, 796, 827, 2289
BfuI GTATCC 1 cut(s) 130
BglII AGATCT 4 cut(s) 895, 1462, 1581, 1818
BisI GCNGC 4 cut(s) 87, 90, 315, 941
BlsI GCNGC 4 cut(s) 88, 91, 316, 942
Bme18I GGWCC 7 cut(s) 735, 1034, 1085, 1171, 1202, 1787, 1892
BmeRI GACNNNNNGTC 1 cut(s) 1890
BmeT110I CYCGRG 1 cut(s) 1217
BmgT120I GGNCC 9 cut(s) 735, 849, 1034, 1085, 1171, 1202, 1787, 1877, 1892
BmiI GGNNCC 8 cut(s) 1035, 1036, 1172, 1203, 1967, 2138, 2254, 2404
BmrI ACTGGG 1 cut(s) 2344
BmsI GCATC 4 cut(s) 1130, 1375, 2044, 2420
BmuI ACTGGG 1 cut(s) 2344
BpiI GAAGAC 2 cut(s) 549, 2373
BpmI CTGGAG 2 cut(s) 449, 1560
Bpu14I TTCGAA 1 cut(s) 2232
BpuEI CTTGAG 3 cut(s) 1472, 1598, 1691
BpuMI CCSGG 2 cut(s) 1218, 1219
BsaBI GATNNNNATC 1 cut(s) 971
BsaI GGTCTC 2 cut(s) 1490, 1877
BsaWI WCCGGW 2 cut(s) 126, 749
BsaXI ACNNNNNCTCC 2 cut(s) 2363, 2393
Bsc4I CCNNNNNNNGG 6 cut(s) 133, 554, 581, 732, 2097, 2133
Bse118I RCCGGY 2 cut(s) 749, 1556
Bse1I ACTGG 4 cut(s) 187, 1894, 1969, 2350
Bse21I CCTNAGG 1 cut(s) 2175
Bse3DI GCAATG 1 cut(s) 99
Bse8I GATNNNNATC 1 cut(s) 971
BseAI TCCGGA 1 cut(s) 126
BseBI CCWGG 8 cut(s) 282, 467, 566, 937, 1040, 2095, 2250, 2408
BseJI GATNNNNATC 1 cut(s) 971
BseLI CCNNNNNNNGG 6 cut(s) 133, 554, 581, 732, 2097, 2133
BseMI GCAATG 1 cut(s) 99
BseMII CTCAG 8 cut(s) 294, 372, 567, 1016, 1872, 2129, 2166, 2429
BseNI ACTGG 4 cut(s) 187, 1894, 1969, 2350
BseRI GAGGAG 2 cut(s) 1547, 2082
BseSI GKGCMC 1 cut(s) 340
BseXI GCAGC 4 cut(s) 73, 76, 301, 927
BseYI CCCAGC 3 cut(s) 718, 1163, 1961
BsgI GTGCAG 1 cut(s) 165
BshFI GGCC 9 cut(s) 421, 485, 850, 1152, 1766, 1779, 1826, 1878, 2248
BshNI GGYRCC 1 cut(s) 2252
BshTI ACCGGT 1 cut(s) 749
BsiHKAI GWGCWC 4 cut(s) 340, 393, 1548, 2185
BsiHKCI CYCGRG 1 cut(s) 1217
BsiSI CCGG 4 cut(s) 127, 750, 1218, 1557
BslFI GGGAC 4 cut(s) 1020, 1215, 1687, 1856
BslI CCNNNNNNNGG 6 cut(s) 133, 554, 581, 732, 2097, 2133
BsmAI GTCTC 5 cut(s) 377, 1423, 1490, 1655, 1877
BsmFI GGGAC 4 cut(s) 1020, 1215, 1687, 1856
BsmI GAATGC 2 cut(s) 520, 1360
BsnI GGCC 9 cut(s) 421, 485, 850, 1152, 1766, 1779, 1826, 1878, 2248
Bso31I GGTCTC 2 cut(s) 1490, 1877
BsoBI CYCGRG 1 cut(s) 1217
Bsp119I TTCGAA 1 cut(s) 2232
Bsp1286I GDGCHC 4 cut(s) 340, 393, 1548, 2185
Bsp13I TCCGGA 1 cut(s) 126
Bsp1407I TGTACA 3 cut(s) 1249, 1725, 2262
BspACI CCGC 2 cut(s) 2091, 2468
BspANI GGCC 9 cut(s) 421, 485, 850, 1152, 1766, 1779, 1826, 1878, 2248
BspCNI CTCAG 8 cut(s) 295, 373, 568, 1015, 1873, 2128, 2167, 2428
BspEI TCCGGA 1 cut(s) 126
BspLI GGNNCC 8 cut(s) 1035, 1036, 1172, 1203, 1967, 2138, 2254, 2404
BspMI ACCTGC 4 cut(s) 770, 796, 827, 2289
BspPI GGATC 9 cut(s) 422, 1070, 1239, 1344, 1502, 1602, 1793, 1857, 2381
BspQI GCTCTTC 1 cut(s) 324
BspT104I TTCGAA 1 cut(s) 2232
BspT107I GGYRCC 1 cut(s) 2252
BspTNI GGTCTC 2 cut(s) 1490, 1877
BsrDI GCAATG 1 cut(s) 99
BsrFI RCCGGY 2 cut(s) 749, 1556
BsrGI TGTACA 3 cut(s) 1249, 1725, 2262
BsrI ACTGG 4 cut(s) 187, 1894, 1969, 2350
BssAI RCCGGY 2 cut(s) 749, 1556
BssT1I CCWWGG 4 cut(s) 402, 1323, 1790, 2132
Bst2UI CCWGG 8 cut(s) 282, 467, 566, 937, 1040, 2095, 2250, 2408
Bst6I CTCTTC 4 cut(s) 324, 474, 555, 914
BstAUI TGTACA 3 cut(s) 1249, 1725, 2262
BstBI TTCGAA 1 cut(s) 2232
BstC8I GCNNGC 5 cut(s) 226, 767, 945, 949, 1738
BstENI CCTNNNNNAGG 1 cut(s) 579
BstMAI GTCTC 5 cut(s) 377, 1423, 1490, 1655, 1877
BstMWI GCNNNNNNNGC 4 cut(s) 152, 599, 937, 953
BstNI CCWGG 8 cut(s) 282, 467, 566, 937, 1040, 2095, 2250, 2408
BstNSI RCATGY 3 cut(s) 290, 1915, 2228
BstSFI CTRYAG 1 cut(s) 2197
BstSLI GKGCMC 1 cut(s) 340
BstV1I GCAGC 4 cut(s) 73, 76, 301, 927
BstV2I GAAGAC 2 cut(s) 549, 2373
BstX2I RGATCY 7 cut(s) 895, 1231, 1462, 1581, 1594, 1818, 1849
BstXI CCANNNNNNTGG 2 cut(s) 473, 1822
BstYI RGATCY 7 cut(s) 895, 1231, 1462, 1581, 1594, 1818, 1849
Bsu36I CCTNAGG 1 cut(s) 2175
BsuI GTATCC 1 cut(s) 130
BsuRI GGCC 9 cut(s) 421, 485, 850, 1152, 1766, 1779, 1826, 1878, 2248
BtsI GCAGTG 1 cut(s) 951
BtsIMutI CAGTG 9 cut(s) 951, 1314, 1451, 1695, 1887, 1976, 2206, 2258, 2357
BveI ACCTGC 4 cut(s) 770, 796, 827, 2289
Cac8I GCNNGC 5 cut(s) 226, 767, 945, 949, 1738
CaiI CAGNNNCTG 1 cut(s) 1071
Cfr10I RCCGGY 2 cut(s) 749, 1556
Cfr13I GGNCC 9 cut(s) 735, 849, 1034, 1085, 1171, 1202, 1787, 1877, 1892
Cfr9I CCCGGG 1 cut(s) 1217
CseI GACGC 1 cut(s) 1481
Csp6I GTAC 7 cut(s) 256, 1250, 1686, 1726, 1930, 2253, 2263
CspAI ACCGGT 1 cut(s) 749
CviQI GTAC 7 cut(s) 256, 1250, 1686, 1726, 1930, 2253, 2263
DraI TTTAAA 1 cut(s) 664
DrdI GACNNNNNNGTC 2 cut(s) 80, 214
DriI GACNNNNNGTC 1 cut(s) 1890
DseDI GACNNNNNNGTC 2 cut(s) 80, 214
EaeI YGGCCR 3 cut(s) 1150, 1777, 1824
Eam1104I CTCTTC 4 cut(s) 324, 474, 555, 914
Eam1105I GACNNNNNGTC 1 cut(s) 1890
EarI CTCTTC 4 cut(s) 324, 474, 555, 914
Eco130I CCWWGG 4 cut(s) 402, 1323, 1790, 2132
Eco147I AGGCCT 3 cut(s) 421, 485, 2248
Eco31I GGTCTC 2 cut(s) 1490, 1877
Eco47I GGWCC 7 cut(s) 735, 1034, 1085, 1171, 1202, 1787, 1892
Eco57I CTGAAG 4 cut(s) 1443, 1583, 2019, 2316
Eco81I CCTNAGG 1 cut(s) 2175
Eco88I CYCGRG 1 cut(s) 1217
EcoNI CCTNNNNNAGG 1 cut(s) 579
EcoO109I RGGNCCY 1 cut(s) 1034
EcoRII CCWGG 8 cut(s) 280, 465, 564, 935, 1038, 2093, 2248, 2406
EcoT14I CCWWGG 4 cut(s) 402, 1323, 1790, 2132
ErhI CCWWGG 4 cut(s) 402, 1323, 1790, 2132
FalI AAGNNNNNCTT 4 cut(s) 534, 566, 1740, 1772
FaqI GGGAC 4 cut(s) 1020, 1215, 1687, 1856
FauI CCCGC 1 cut(s) 2475
FbaI TGATCA 2 cut(s) 1045, 2362
FblI GTMKAC 1 cut(s) 570
Fnu4HI GCNGC 4 cut(s) 87, 90, 315, 941
Fsp4HI GCNGC 4 cut(s) 87, 90, 315, 941
GluI GCNGC 4 cut(s) 87, 90, 315, 941
GsaI CCCAGC 3 cut(s) 722, 1167, 1965
GsuI CTGGAG 2 cut(s) 449, 1560
HaeIII GGCC 9 cut(s) 421, 485, 850, 1152, 1766, 1779, 1826, 1878, 2248
HapII CCGG 4 cut(s) 127, 750, 1218, 1557
HgaI GACGC 1 cut(s) 1481
HincII GTYRAC 5 cut(s) 180, 292, 429, 436, 1909
HindII GTYRAC 5 cut(s) 180, 292, 429, 436, 1909
HindIII AAGCTT 3 cut(s) 226, 863, 1472
HpaI GTTAAC 1 cut(s) 436
HpaII CCGG 4 cut(s) 127, 750, 1218, 1557
Hpy188III TCNNGA 8 cut(s) 127, 1079, 1267, 1334, 1539, 1601, 2334, 2426
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 599, 937, 953
KflI GGGWCCC 1 cut(s) 1034
Kpn2I TCCGGA 1 cut(s) 126
KpnI GGTACC 1 cut(s) 2256
Ksp22I TGATCA 2 cut(s) 1045, 2362
KspAI GTTAAC 1 cut(s) 436
LguI GCTCTTC 1 cut(s) 324
LmnI GCTCC 4 cut(s) 396, 1619, 2142, 2455
Lsp1109I GCAGC 4 cut(s) 73, 76, 301, 927
LweI GCATC 4 cut(s) 1130, 1375, 2044, 2420
MaeIII GTNAC 3 cut(s) 307, 884, 1310
MflI RGATCY 7 cut(s) 895, 1231, 1462, 1581, 1594, 1818, 1849
MhlI GDGCHC 4 cut(s) 340, 393, 1548, 2185
MluCI AATT 7 cut(s) 171, 493, 1626, 1806, 1832, 2163, 2238
MlyI GAGTC 4 cut(s) 68, 224, 1544, 1943
MmeI TCCRAC 2 cut(s) 2111, 2373
MroI TCCGGA 1 cut(s) 126
MroXI GAANNNNTTC 1 cut(s) 2238
MseI TTAA 5 cut(s) 174, 435, 629, 663, 1935
MslI CAYNNNNRTG 1 cut(s) 2052
MspA1I CMGCKG 1 cut(s) 2419
MspI CCGG 4 cut(s) 127, 750, 1218, 1557
Mva1269I GAATGC 2 cut(s) 520, 1360
MvaI CCWGG 8 cut(s) 282, 467, 566, 937, 1040, 2095, 2250, 2408
MwoI GCNNNNNNNGC 4 cut(s) 152, 599, 937, 953
NciI CCSGG 2 cut(s) 1218, 1219
NlaIV GGNNCC 8 cut(s) 1035, 1036, 1172, 1203, 1967, 2138, 2254, 2404
NmeAIII GCCGAG 1 cut(s) 1852
NmuCI GTSAC 1 cut(s) 884
NspI RCATGY 3 cut(s) 290, 1915, 2228
NspV TTCGAA 1 cut(s) 2232
PasI CCCWGGG 1 cut(s) 281
PceI AGGCCT 3 cut(s) 421, 485, 2248
PciI ACATGT 2 cut(s) 1911, 2224
PciSI GCTCTTC 1 cut(s) 324
PctI GAATGC 2 cut(s) 520, 1360
PdmI GAANNNNTTC 1 cut(s) 2238
PfeI GAWTC 8 cut(s) 186, 275, 870, 1021, 1114, 1194, 1379, 2294
PflMI CCANNNNNTGG 1 cut(s) 732
PinAI ACCGGT 1 cut(s) 749
PkrI GCNGC 4 cut(s) 88, 91, 316, 942
PleI GAGTC 4 cut(s) 68, 223, 1543, 1943
PpsI GAGTC 4 cut(s) 68, 223, 1543, 1943
PpuMI RGGWCCY 1 cut(s) 1034
PscI ACATGT 2 cut(s) 1911, 2224
Psp5II RGGWCCY 1 cut(s) 1034
Psp6I CCWGG 8 cut(s) 280, 465, 564, 935, 1038, 2093, 2248, 2406
PspFI CCCAGC 3 cut(s) 718, 1163, 1961
PspGI CCWGG 8 cut(s) 280, 465, 564, 935, 1038, 2093, 2248, 2406
PspN4I GGNNCC 8 cut(s) 1035, 1036, 1172, 1203, 1967, 2138, 2254, 2404
PspPI GGNCC 9 cut(s) 735, 849, 1034, 1085, 1171, 1202, 1787, 1877, 1892
PspPPI RGGWCCY 1 cut(s) 1034
PstNI CAGNNNCTG 1 cut(s) 1071
PsuI RGATCY 7 cut(s) 895, 1231, 1462, 1581, 1594, 1818, 1849
PvuII CAGCTG 1 cut(s) 2419
RsaI GTAC 7 cut(s) 257, 1251, 1687, 1727, 1931, 2254, 2264
RsaNI GTAC 7 cut(s) 256, 1250, 1686, 1726, 1930, 2253, 2263
RseI CAYNNNNRTG 1 cut(s) 2052
SapI GCTCTTC 1 cut(s) 324
SaqAI TTAA 5 cut(s) 174, 435, 629, 663, 1935
SatI GCNGC 4 cut(s) 87, 90, 315, 941
Sau96I GGNCC 9 cut(s) 735, 849, 1034, 1085, 1171, 1202, 1787, 1877, 1892
SchI GAGTC 4 cut(s) 68, 224, 1544, 1943
SduI GDGCHC 4 cut(s) 340, 393, 1548, 2185
SfaNI GCATC 4 cut(s) 1130, 1375, 2044, 2420
SfcI CTRYAG 1 cut(s) 2197
SfuI TTCGAA 1 cut(s) 2232
SinI GGWCC 7 cut(s) 735, 1034, 1085, 1171, 1202, 1787, 1892
SmaI CCCGGG 1 cut(s) 1219
SmiMI CAYNNNNRTG 1 cut(s) 2052
SmlI CTYRAG 3 cut(s) 1487, 1577, 1670
SmoI CTYRAG 3 cut(s) 1487, 1577, 1670
Sse9I AATT 7 cut(s) 171, 493, 1626, 1806, 1832, 2163, 2238
SseBI AGGCCT 3 cut(s) 421, 485, 2248
SsiI CCGC 2 cut(s) 2091, 2468
StuI AGGCCT 3 cut(s) 421, 485, 2248
StyI CCWWGG 4 cut(s) 402, 1323, 1790, 2132
TaqI TCGA 2 cut(s) 48, 2232
TasI AATT 7 cut(s) 171, 493, 1626, 1806, 1832, 2163, 2238
TatI WGTACW 3 cut(s) 1249, 1725, 2262
TfiI GAWTC 8 cut(s) 186, 275, 870, 1021, 1114, 1194, 1379, 2294
Tru1I TTAA 5 cut(s) 174, 435, 629, 663, 1935
Tru9I TTAA 5 cut(s) 174, 435, 629, 663, 1935
TscAI CASTG 9 cut(s) 958, 1321, 1451, 1695, 1894, 1976, 2206, 2265, 2357
TseFI GTSAC 1 cut(s) 884
TseI GCWGC 4 cut(s) 86, 89, 314, 940
Tsp45I GTSAC 1 cut(s) 884
TspMI CCCGGG 1 cut(s) 1217
TspRI CASTG 9 cut(s) 958, 1321, 1451, 1695, 1894, 1976, 2206, 2265, 2357
Van91I CCANNNNNTGG 1 cut(s) 732
VneI GTGCAC 1 cut(s) 336
VpaK11BI GGWCC 7 cut(s) 735, 1034, 1085, 1171, 1202, 1787, 1892
XagI CCTNNNNNAGG 1 cut(s) 579
XapI RAATTY 5 cut(s) 493, 1806, 1832, 2163, 2238
XceI RCATGY 3 cut(s) 290, 1915, 2228
XcmI CCANNNNNNNNNTGG 1 cut(s) 50
XmaI CCCGGG 1 cut(s) 1217
XmiI GTMKAC 1 cut(s) 570
XmnI GAANNNNTTC 1 cut(s) 2238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.