MD10G1203300.v1.1

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0001995
Physical Location & Seq
Reverse (-)
30097037 .. 30101104
4068 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1203300.v1.1.491

Sequence Viewer

Length: 2532 bp
ATGGTGAAGTTCACAGCAGAGGAGCTCCGTAGGATTATGGACTACAAACATAACATCCGTAATATGTCTGTTATTGCGCACGTCGACCATGGGAAGTCAACCCTTACAGACTCTCTTGTTGCTGCTGCTGGTATCATTGCACAAGAAGTCGCCGGTGATGTCCGTATGACGGATACCCGTGCCGATGAAGCTGAGCGTGGTATCACAATTAAATCTACCGGTATCTCTCTGTACTATGAGATGACTGATGAGTCTTTGCAGAGGTTCAAGGGAGAGAGAAACGGAAATGAGTACCTCATCAATCTCATTGATTCCCCTGGGCACGTTGACTTTTCATCTGAGGTCACAGCTGCTCTGCGTATTACTGATGGTGCACTTGTGGTGGTGGATTGTATTGAGGGTGTGTGTGTCCAGACTGAGACTGTGCTCCGTCAAGCCTTGGGAGAGAGGATCAGGCCTGTTTTGACCGTTAACAAGATGGACAGGTGCTTCCTCGAGCTCCAGGTTGATGGAGAGGAGGCTTACCAGACATTCCAAAGGGTTATTGAGAATGCCAATGTCATTATGGCTACATATGAAGATCCACTTCTTGGTGATGTTCAGGTCTACCCAGAGAAAGGAACAGTCGCCTTCTCAGCTGGTTTGCACGGTTGGGCTTTCACTCTGACCAACTTCGCTAAGATGTATGCTTCCAAGTTTGGAGTTGATGAGTCAAAGATGATGGAAAGGCTCTGGGGTGAGAACTATTTTGACCCAGCTACGAAGAAATGGACCAGCAAGAACACCGGAACTGCAACCTGCAAGCGTGGTTTCGTTCAGTTCTGTTATGAGCCCATCAAGCAGATCATCAACACGTGCATGAATGATCAGAAGGATAAGTTGTGGCCCATGTTGACAAAGCTTGGTGTCACCATGAAGAGTGATGAGAAGGACCTGATGGGGAAGGCATTGATGAAGCGTGTTATGCAGACCTGGCTACCAGCTAGCACTGCTCTATTGGAAATGATGATCTTTCACCTTCCATCTCCTTCCACGGCTCAAAGGTACCGTGTTGAGAATTTGTATGAGGGTCCCCTTGATGATCAATATGCTAATGCCATCAGGAACTGTGACCCCGAAGGACCTCTTATGCTCTATGTGTCTAAGATGATTCCCGCTTCTGATAAGGGTAGATTCTTTGCCTTTGGCCGTGTCTTTGCTGGTAAAGTTCAGACAGGTTTGAAGGTTAGAATCATGGGTCCAAATTTTGTTCCTGGAGAGAAGAAGGATTTGTATGTCAAGAATGTACAGAGGACTGTTATTTGGATGGGAAAGAGACAAGAAACTGTTGAGGATGTTCCTTGCGGTAACACTGTTGCCTTGGTCGGTTTGGATCAGTTTATCACCAAGAATGCTACTTTAACAAATGAGAAGGAAGTGGATGCTCACCCTATTCGTGCTATGAAGTTCTCAGTTTCACCCGTCGTGCGTGTTGCTGTCCAGTGCAAGGTTGCATCTGACCTTCCCAAACTTGTTGAAGGTCTCAAGCGTTTGGCCAAGTCTGATCCTATGGTTGTCTGCTCCATTGAGGAGTCGGGAGAGCACATTATTGCTGGTGCTGGTGAACTGCATCTTGAGATCTGTTTGAAGGATCTGCAAGATGATTTTATGGGTGGTGCTGAGATTATAAAGTCTGACCCTGTTGTGTCCTTCCGTGAGACTGTCCTTGATAAGTCCTGCCGTACAGTGATGAGCAAGTCTCCAAACAAGCATAACCGTCTGTACATGGAAGCAAGGCCCCTTGAGGAAGGGCTTCCTGAGGCTATTGATGAAGGTCGCATTGGCCCAAGAGATGATCCCAAGATTCGTTCCAAGATCTTGGCTGAGGAGTTTGGTTGGGACAAGGATCTTGCTAAGAAAATCTGGTGTTTTGGTCCCGAGACCACTGGTCCTAACATGGTGGTCGACATGTGTAAGGGAGTCCAGTACCTTAATGAAATCAAGGATTCAGTTGTTGCTGGGTTCCAGTGGGCCTCAAAGGAAGGTGCATTGGCAGAAGAAAACATGAGAGGTATTTGCTTTGAAGTTTGTGATGTGGTTCTTCACGCTGATGCCATCCACAGAGGGGGAGGTCAGGTCATTCCAACTGCTCGGAGGGTCATCTATGCCTCTCAGCTCACAGCCAAGCCAAGGCTTCTCGAACCAGTGTATCTTGTTGAAATTCAAGCTCCAGAGGGGGCTCTTGGAGGTATATACAGTGTTCTGAATCAGAAACGTGGACACGTGTTTGAGGAGATGCAGAGGCCGGGAACTCCACTTTACAACATCAAGGCATACCTCCCCGTTGTCGAGTCCTTTGGATTCTCTGGTCAACTGAGGGCTGCAACTTCTGGACAGGCTTTCCCACAATGTGTGTTTGATCATTGGGAGATGATGTCGTCTGATCCATTGGAAGCTGGATCCCAGGCTGCAGCACTTGTTACCGACATCCGTAAGAGGAAGGGATTGAAGGAGCAGATGACCCCACTATCCGAGTTTGAGGACAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04152 ko04921 map04152 map04921
Pfam Domains
Protein Families
EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

844

Amino Acids

94.05

Weight (kDa)

5.8

Isoelectric Point (pI)

33.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 18 - 221 1.4e-48 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 379 - 469 4.1e-06 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 394 - 462 5e-08 Elongation factor Tu domain 2
EFG_III PF14492 487 - 549 1e-11 Elongation Factor G, domain III
EFG_IV PF03764 610 - 722 2e-29 Elongation factor G, domain IV
EFG_C PF00679 724 - 811 1.9e-20 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1667
AasI GACNNNNNNGTC 1 cut(s) 250
Acc16I TGCGCA 1 cut(s) 78
Acc36I ACCTGC 1 cut(s) 806
Acc65I GGTACC 1 cut(s) 1044
AccB1I GGYRCC 1 cut(s) 1044
AccB7I CCANNNNNTGG 1 cut(s) 590
AccI GTMKAC 3 cut(s) 84, 606, 1944
AciI CCGC 2 cut(s) 1155, 1344
AcoI YGGCCR 2 cut(s) 1186, 1533
AcsI RAATTY 3 cut(s) 1057, 1243, 2199
AcvI CACGTG 2 cut(s) 855, 2263
AdeI CACNNNGTG 1 cut(s) 2390
AfaI GTAC 7 cut(s) 233, 293, 1046, 1287, 1723, 1763, 1967
AfiI CCNNNNNNNGG 6 cut(s) 169, 590, 617, 1486, 2104, 2169
AflIII ACRYGT 4 cut(s) 852, 1947, 2260, 2262
AgeI ACCGGT 1 cut(s) 218
AgsI TTSAA 8 cut(s) 268, 1222, 1517, 1627, 2063, 2198, 2204, 2488
AhdI GACNNNNNGTC 1 cut(s) 1926
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 5 cut(s) 316, 501, 971, 1252, 2442
AjuI GAANNNNNNNTTGG 2 cut(s) 1803, 1835
Alw21I GWGCWC 5 cut(s) 27, 376, 429, 501, 1584
Alw26I GTCTC 6 cut(s) 413, 1309, 1526, 1691, 1743, 1913
Alw44I GTGCAC 1 cut(s) 372
AlwNI CAGNNNCTG 1 cut(s) 1107
Ama87I CYCGRG 2 cut(s) 494, 1916
AoxI GGCC 8 cut(s) 455, 884, 1186, 1533, 1775, 1822, 2010, 2282
ApaLI GTGCAC 1 cut(s) 372
ApeKI GCWGC 6 cut(s) 122, 125, 350, 2360, 2447, 2450
ApoI RAATTY 3 cut(s) 1057, 1243, 2199
AsiGI ACCGGT 1 cut(s) 218
Asp700I GAANNNNTTC 1 cut(s) 1791
Asp718I GGTACC 1 cut(s) 1044
AspLEI GCGC 1 cut(s) 79
AsuC2I CCSGG 1 cut(s) 2286
AsuNHI GCTAGC 1 cut(s) 983
AvaI CYCGRG 2 cut(s) 494, 1916
AvaII GGWCC 7 cut(s) 771, 931, 1070, 1121, 1238, 1913, 1928
AxyI CCTNAGG 1 cut(s) 1797
BaeGI GKGCMC 2 cut(s) 324, 376
BaeI ACNNNNGTAYC 2 cut(s) 2171, 2204
BalI TGGCCA 1 cut(s) 1535
BamHI GGATCC 1 cut(s) 2438
BanI GGYRCC 1 cut(s) 1044
BanII GRGCYC 4 cut(s) 27, 501, 834, 2221
BbrPI CACGTG 2 cut(s) 855, 2263
Bbv12I GWGCWC 5 cut(s) 27, 376, 429, 501, 1584
BbvCI CCTCAGC 1 cut(s) 1863
BbvI GCAGC 6 cut(s) 109, 112, 337, 2347, 2434, 2462
BceAI ACGGC 3 cut(s) 1050, 1173, 1704
BciT130I CCWGG 5 cut(s) 318, 503, 973, 1254, 2444
BciVI GTATCC 1 cut(s) 166
BclI TGATCA 3 cut(s) 865, 1081, 2398
BcnI CCSGG 1 cut(s) 2286
BcoDI GTCTC 6 cut(s) 413, 1309, 1526, 1691, 1743, 1913
BfaI CTAG 1 cut(s) 984
BfmI CTRYAG 1 cut(s) 2448
BfuAI ACCTGC 1 cut(s) 806
BfuI GTATCC 1 cut(s) 166
BglII AGATCT 2 cut(s) 1617, 1854
BisI GCNGC 6 cut(s) 123, 126, 351, 2361, 2448, 2451
BlpI GCTNAGC 1 cut(s) 192
BlsI GCNGC 6 cut(s) 124, 127, 352, 2362, 2449, 2452
Bme1390I CCNGG 6 cut(s) 318, 503, 973, 1254, 2286, 2444
Bme18I GGWCC 7 cut(s) 771, 931, 1070, 1121, 1238, 1913, 1928
BmeRI GACNNNNNGTC 1 cut(s) 1926
BmeT110I CYCGRG 2 cut(s) 494, 1916
BmgBI CACGTC 1 cut(s) 82
BmiI GGNNCC 8 cut(s) 1046, 1071, 1072, 1239, 1778, 1915, 2003, 2440
BmrFI CCNGG 6 cut(s) 318, 503, 973, 1254, 2286, 2444
BmsI GCATC 5 cut(s) 1411, 1502, 1618, 2080, 2265
BmtI GCTAGC 1 cut(s) 987
BplI GAGNNNNNCTC 2 cut(s) 1723, 1755
BpmI CTGGAG 3 cut(s) 485, 1275, 2193
Bpu10I CCTNAGC 1 cut(s) 1863
Bpu1102I GCTNAGC 1 cut(s) 192
BpuEI CTTGAG 3 cut(s) 1508, 1634, 1802
BpuMI CCSGG 1 cut(s) 2286
BsaAI YACGTR 2 cut(s) 855, 2263
BsaI GGTCTC 2 cut(s) 1526, 1913
BsaJI CCNNGG 8 cut(s) 88, 316, 317, 438, 1032, 1359, 2168, 2442
BsaWI WCCGGW 2 cut(s) 218, 785
BsaXI ACNNNNNCTCC 4 cut(s) 2100, 2130, 2399, 2429
Bsc4I CCNNNNNNNGG 6 cut(s) 169, 590, 617, 1486, 2104, 2169
Bse118I RCCGGY 2 cut(s) 152, 218
Bse1I ACTGG 5 cut(s) 1480, 1930, 1963, 2005, 2183
Bse21I CCTNAGG 1 cut(s) 1797
Bse3DI GCAATG 1 cut(s) 135
BseBI CCWGG 5 cut(s) 318, 503, 973, 1254, 2444
BseDI CCNNGG 8 cut(s) 88, 316, 317, 438, 1032, 1359, 2168, 2442
BseGI GGATG 6 cut(s) 54, 1311, 1339, 1426, 2094, 2466
BseLI CCNNNNNNNGG 6 cut(s) 169, 590, 617, 1486, 2104, 2169
BseMI GCAATG 1 cut(s) 135
BseNI ACTGG 5 cut(s) 1480, 1930, 1963, 2005, 2183
BseRI GAGGAG 5 cut(s) 35, 530, 1583, 1880, 2285
BseSI GKGCMC 2 cut(s) 324, 376
BseXI GCAGC 6 cut(s) 109, 112, 337, 2347, 2434, 2462
BseYI CCCAGC 2 cut(s) 754, 1997
BshFI GGCC 8 cut(s) 457, 886, 1188, 1535, 1777, 1824, 2012, 2284
BshNI GGYRCC 1 cut(s) 1044
BshTI ACCGGT 1 cut(s) 218
BsiHKAI GWGCWC 5 cut(s) 27, 376, 429, 501, 1584
BsiHKCI CYCGRG 2 cut(s) 494, 1916
BsiSI CCGG 4 cut(s) 153, 219, 786, 2285
BslFI GGGAC 3 cut(s) 1056, 1892, 1899
BslI CCNNNNNNNGG 6 cut(s) 169, 590, 617, 1486, 2104, 2169
BsmAI GTCTC 6 cut(s) 413, 1309, 1526, 1691, 1743, 1913
BsmFI GGGAC 3 cut(s) 1056, 1892, 1899
BsmI GAATGC 2 cut(s) 556, 1396
BsnI GGCC 8 cut(s) 457, 886, 1188, 1535, 1777, 1824, 2012, 2284
Bso31I GGTCTC 2 cut(s) 1526, 1913
BsoBI CYCGRG 2 cut(s) 494, 1916
Bsp1286I GDGCHC 8 cut(s) 27, 324, 376, 429, 501, 834, 1584, 2221
Bsp1407I TGTACA 2 cut(s) 1285, 1761
Bsp1720I GCTNAGC 1 cut(s) 192
Bsp19I CCATGG 1 cut(s) 88
BspACI CCGC 2 cut(s) 1155, 1344
BspANI GGCC 8 cut(s) 457, 886, 1188, 1535, 1777, 1824, 2012, 2284
BspLI GGNNCC 8 cut(s) 1046, 1071, 1072, 1239, 1778, 1915, 2003, 2440
BspMAI CTGCAG 1 cut(s) 2452
BspMI ACCTGC 1 cut(s) 806
BspOI GCTAGC 1 cut(s) 987
BspT107I GGYRCC 1 cut(s) 1044
BspTNI GGTCTC 2 cut(s) 1526, 1913
BsrDI GCAATG 1 cut(s) 135
BsrFI RCCGGY 2 cut(s) 152, 218
BsrGI TGTACA 2 cut(s) 1285, 1761
BsrI ACTGG 5 cut(s) 1480, 1930, 1963, 2005, 2183
BssAI RCCGGY 2 cut(s) 152, 218
BssECI CCNNGG 8 cut(s) 88, 316, 317, 438, 1032, 1359, 2168, 2442
BssT1I CCWWGG 4 cut(s) 88, 438, 1359, 2168
Bst2UI CCWGG 5 cut(s) 318, 503, 973, 1254, 2444
Bst6I CTCTTC 1 cut(s) 911
BstAUI TGTACA 2 cut(s) 1285, 1761
BstBAI YACGTR 2 cut(s) 855, 2263
BstC8I GCNNGC 2 cut(s) 803, 985
BstDSI CCRYGG 2 cut(s) 88, 1032
BstF5I GGATG 6 cut(s) 54, 1311, 1339, 1426, 2094, 2466
BstHHI GCGC 1 cut(s) 79
BstMAI GTCTC 6 cut(s) 413, 1309, 1526, 1691, 1743, 1913
BstMWI GCNNNNNNNGC 6 cut(s) 188, 635, 838, 964, 973, 989
BstNI CCWGG 5 cut(s) 318, 503, 973, 1254, 2444
BstNSI RCATGY 1 cut(s) 1951
BstSCI CCNGG 6 cut(s) 316, 501, 971, 1252, 2284, 2442
BstSFI CTRYAG 1 cut(s) 2448
BstSLI GKGCMC 2 cut(s) 324, 376
BstV1I GCAGC 6 cut(s) 109, 112, 337, 2347, 2434, 2462
BstX2I RGATCY 6 cut(s) 580, 1617, 1630, 1854, 1885, 2438
BstXI CCANNNNNNTGG 2 cut(s) 509, 1858
BstYI RGATCY 6 cut(s) 580, 1617, 1630, 1854, 1885, 2438
Bsu36I CCTNAGG 1 cut(s) 1797
BsuI GTATCC 1 cut(s) 166
BsuRI GGCC 8 cut(s) 457, 886, 1188, 1535, 1777, 1824, 2012, 2284
BtgI CCRYGG 2 cut(s) 88, 1032
BtrI CACGTC 1 cut(s) 82
BtsCI GGATG 6 cut(s) 54, 1311, 1339, 1426, 2094, 2466
BtsI GCAGTG 1 cut(s) 987
BtsIMutI CAGTG 8 cut(s) 987, 1350, 1487, 1731, 1923, 2012, 2190, 2242
BveI ACCTGC 1 cut(s) 806
Cac8I GCNNGC 2 cut(s) 803, 985
CaiI CAGNNNCTG 1 cut(s) 1107
CfoI GCGC 1 cut(s) 79
Cfr10I RCCGGY 2 cut(s) 152, 218
Csp6I GTAC 7 cut(s) 232, 292, 1045, 1286, 1722, 1762, 1966
CspAI ACCGGT 1 cut(s) 218
CviAII CATG 9 cut(s) 89, 859, 889, 913, 1234, 1765, 1936, 1948, 2044
CviQI GTAC 7 cut(s) 232, 292, 1045, 1286, 1722, 1762, 1966
DraIII CACNNNGTG 1 cut(s) 2390
DrdI GACNNNNNNGTC 1 cut(s) 250
DriI GACNNNNNGTC 1 cut(s) 1926
DseDI GACNNNNNNGTC 1 cut(s) 250
EaeI YGGCCR 2 cut(s) 1186, 1533
Eam1104I CTCTTC 1 cut(s) 911
Eam1105I GACNNNNNGTC 1 cut(s) 1926
EarI CTCTTC 1 cut(s) 911
Ecl136II GAGCTC 2 cut(s) 25, 499
Eco130I CCWWGG 4 cut(s) 88, 438, 1359, 2168
Eco147I AGGCCT 1 cut(s) 457
Eco24I GRGCYC 4 cut(s) 27, 501, 834, 2221
Eco31I GGTCTC 2 cut(s) 1526, 1913
Eco47I GGWCC 7 cut(s) 771, 931, 1070, 1121, 1238, 1913, 1928
Eco53kI GAGCTC 2 cut(s) 25, 499
Eco72I CACGTG 2 cut(s) 855, 2263
Eco81I CCTNAGG 1 cut(s) 1797
Eco88I CYCGRG 2 cut(s) 494, 1916
EcoICRI GAGCTC 2 cut(s) 25, 499
EcoO109I RGGNCCY 4 cut(s) 931, 1070, 1121, 1776
EcoRII CCWGG 5 cut(s) 316, 501, 971, 1252, 2442
EcoT14I CCWWGG 4 cut(s) 88, 438, 1359, 2168
EcoT38I GRGCYC 4 cut(s) 27, 501, 834, 2221
ErhI CCWWGG 4 cut(s) 88, 438, 1359, 2168
FaeI CATG 9 cut(s) 92, 862, 892, 916, 1237, 1768, 1939, 1951, 2047
FalI AAGNNNNNCTT 4 cut(s) 570, 602, 1110, 1142
FaqI GGGAC 3 cut(s) 1056, 1892, 1899
FatI CATG 9 cut(s) 88, 858, 888, 912, 1233, 1764, 1935, 1947, 2043
FauI CCCGC 1 cut(s) 1162
FauNDI CATATG 1 cut(s) 574
FbaI TGATCA 3 cut(s) 865, 1081, 2398
FblI GTMKAC 3 cut(s) 84, 606, 1944
Fnu4HI GCNGC 6 cut(s) 123, 126, 351, 2361, 2448, 2451
FokI GGATG 6 cut(s) 41, 1318, 1346, 1433, 2081, 2453
FriOI GRGCYC 4 cut(s) 27, 501, 834, 2221
Fsp4HI GCNGC 6 cut(s) 123, 126, 351, 2361, 2448, 2451
FspBI CTAG 1 cut(s) 984
FspI TGCGCA 1 cut(s) 78
GlaI GCGC 1 cut(s) 78
GluI GCNGC 6 cut(s) 123, 126, 351, 2361, 2448, 2451
GsaI CCCAGC 2 cut(s) 758, 2001
GsuI CTGGAG 3 cut(s) 485, 1275, 2193
HaeIII GGCC 8 cut(s) 457, 886, 1188, 1535, 1777, 1824, 2012, 2284
HapII CCGG 4 cut(s) 153, 219, 786, 2285
HhaI GCGC 1 cut(s) 79
Hin1II CATG 9 cut(s) 92, 862, 892, 916, 1237, 1768, 1939, 1951, 2047
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HincII GTYRAC 7 cut(s) 85, 99, 328, 472, 894, 1945, 2351
HindII GTYRAC 7 cut(s) 85, 99, 328, 472, 894, 1945, 2351
HindIII AAGCTT 2 cut(s) 899, 2525
HpaI GTTAAC 1 cut(s) 472
HpaII CCGG 4 cut(s) 153, 219, 786, 2285
Hpy99I CGWCG 2 cut(s) 86, 1466
HpyCH4IV ACGT 5 cut(s) 81, 324, 854, 2254, 2262
HpyF10VI GCNNNNNNNGC 6 cut(s) 188, 635, 838, 964, 973, 989
HpySE526I ACGT 5 cut(s) 81, 324, 854, 2254, 2262
Hsp92II CATG 9 cut(s) 92, 862, 892, 916, 1237, 1768, 1939, 1951, 2047
HspAI GCGC 1 cut(s) 77
KflI GGGWCCC 1 cut(s) 1070
KpnI GGTACC 1 cut(s) 1048
Ksp22I TGATCA 3 cut(s) 865, 1081, 2398
KspAI GTTAAC 1 cut(s) 472
LmnI GCTCC 7 cut(s) 22, 30, 432, 504, 1565, 2212, 2491
Lsp1109I GCAGC 6 cut(s) 109, 112, 337, 2347, 2434, 2462
LweI GCATC 5 cut(s) 1411, 1502, 1618, 2080, 2265
MaeI CTAG 1 cut(s) 984
MaeII ACGT 5 cut(s) 81, 324, 854, 2254, 2262
MaeIII GTNAC 5 cut(s) 343, 907, 1109, 1346, 2458
MboII GAAGA 6 cut(s) 590, 775, 928, 1273, 2048, 2072
MflI RGATCY 6 cut(s) 580, 1617, 1630, 1854, 1885, 2438
MhlI GDGCHC 8 cut(s) 27, 324, 376, 429, 501, 834, 1584, 2221
MlsI TGGCCA 1 cut(s) 1535
MluCI AATT 4 cut(s) 207, 1057, 1243, 2199
MluNI TGGCCA 1 cut(s) 1535
MlyI GAGTC 6 cut(s) 104, 260, 719, 1580, 1968, 2339
MmeI TCCRAC 1 cut(s) 2147
Mox20I TGGCCA 1 cut(s) 1535
MroXI GAANNNNTTC 1 cut(s) 1791
MscI TGGCCA 1 cut(s) 1535
MseI TTAA 4 cut(s) 210, 471, 1400, 1971
MslI CAYNNNNRTG 2 cut(s) 857, 2088
Msp20I TGGCCA 1 cut(s) 1535
MspA1I CMGCKG 2 cut(s) 350, 638
MspI CCGG 4 cut(s) 153, 219, 786, 2285
MspR9I CCNGG 6 cut(s) 318, 503, 973, 1254, 2286, 2444
Mva1269I GAATGC 2 cut(s) 556, 1396
MvaI CCWGG 5 cut(s) 318, 503, 973, 1254, 2444
MwoI GCNNNNNNNGC 6 cut(s) 188, 635, 838, 964, 973, 989
NciI CCSGG 1 cut(s) 2286
NcoI CCATGG 1 cut(s) 88
NdeI CATATG 1 cut(s) 574
NheI GCTAGC 1 cut(s) 983
NlaIII CATG 9 cut(s) 92, 862, 892, 916, 1237, 1768, 1939, 1951, 2047
NlaIV GGNNCC 8 cut(s) 1046, 1071, 1072, 1239, 1778, 1915, 2003, 2440
NmuCI GTSAC 3 cut(s) 343, 907, 1109
NsbI TGCGCA 1 cut(s) 78
NspI RCATGY 1 cut(s) 1951
PaeR7I CTCGAG 1 cut(s) 494
PasI CCCWGGG 1 cut(s) 317
PceI AGGCCT 1 cut(s) 457
PciI ACATGT 1 cut(s) 1947
PctI GAATGC 2 cut(s) 556, 1396
PdmI GAANNNNTTC 1 cut(s) 1791
PfeI GAWTC 8 cut(s) 311, 1150, 1173, 1230, 1843, 1985, 2245, 2340
PflMI CCANNNNNTGG 1 cut(s) 590
PfoI TCCNGGA 1 cut(s) 1252
PinAI ACCGGT 1 cut(s) 218
PkrI GCNGC 6 cut(s) 124, 127, 352, 2362, 2449, 2452
PleI GAGTC 6 cut(s) 104, 259, 718, 1579, 1967, 2338
PmaCI CACGTG 2 cut(s) 855, 2263
PmlI CACGTG 2 cut(s) 855, 2263
PpsI GAGTC 6 cut(s) 104, 259, 718, 1579, 1967, 2338
Ppu21I YACGTR 2 cut(s) 855, 2263
PpuMI RGGWCCY 3 cut(s) 931, 1070, 1121
PscI ACATGT 1 cut(s) 1947
PsiI TTATAA 1 cut(s) 1667
Psp124BI GAGCTC 2 cut(s) 27, 501
Psp5II RGGWCCY 3 cut(s) 931, 1070, 1121
Psp6I CCWGG 5 cut(s) 316, 501, 971, 1252, 2442
PspCI CACGTG 2 cut(s) 855, 2263
PspFI CCCAGC 2 cut(s) 754, 1997
PspGI CCWGG 5 cut(s) 316, 501, 971, 1252, 2442
PspN4I GGNNCC 8 cut(s) 1046, 1071, 1072, 1239, 1778, 1915, 2003, 2440
PspPPI RGGWCCY 3 cut(s) 931, 1070, 1121
PspXI VCTCGAGB 1 cut(s) 494
PstI CTGCAG 1 cut(s) 2452
PstNI CAGNNNCTG 1 cut(s) 1107
PsuI RGATCY 6 cut(s) 580, 1617, 1630, 1854, 1885, 2438
PvuII CAGCTG 2 cut(s) 350, 638
RsaI GTAC 7 cut(s) 233, 293, 1046, 1287, 1723, 1763, 1967
RsaNI GTAC 7 cut(s) 232, 292, 1045, 1286, 1722, 1762, 1966
RseI CAYNNNNRTG 2 cut(s) 857, 2088
SacI GAGCTC 2 cut(s) 27, 501
SalI GTCGAC 2 cut(s) 83, 1943
SaqAI TTAA 4 cut(s) 210, 471, 1400, 1971
SatI GCNGC 6 cut(s) 123, 126, 351, 2361, 2448, 2451
SchI GAGTC 6 cut(s) 104, 260, 719, 1580, 1968, 2339
ScrFI CCNGG 6 cut(s) 318, 503, 973, 1254, 2286, 2444
SduI GDGCHC 8 cut(s) 27, 324, 376, 429, 501, 834, 1584, 2221
SfaNI GCATC 5 cut(s) 1411, 1502, 1618, 2080, 2265
SfcI CTRYAG 1 cut(s) 2448
Sfr274I CTCGAG 1 cut(s) 494
SgrAI CRCCGGYG 1 cut(s) 152
SinI GGWCC 7 cut(s) 771, 931, 1070, 1121, 1238, 1913, 1928
SlaI CTCGAG 1 cut(s) 494
SmiMI CAYNNNNRTG 2 cut(s) 857, 2088
SmlI CTYRAG 4 cut(s) 494, 1523, 1613, 1781
SmoI CTYRAG 4 cut(s) 494, 1523, 1613, 1781
Sse9I AATT 4 cut(s) 207, 1057, 1243, 2199
SseBI AGGCCT 1 cut(s) 457
SsiI CCGC 2 cut(s) 1155, 1344
SspMI CTAG 1 cut(s) 984
SstI GAGCTC 2 cut(s) 27, 501
StuI AGGCCT 1 cut(s) 457
StyD4I CCNGG 6 cut(s) 316, 501, 971, 1252, 2284, 2442
StyI CCWWGG 4 cut(s) 88, 438, 1359, 2168
TaiI ACGT 5 cut(s) 84, 327, 857, 2257, 2265
TaqI TCGA 5 cut(s) 84, 495, 1944, 2178, 2328
TasI AATT 4 cut(s) 207, 1057, 1243, 2199
TatI WGTACW 3 cut(s) 231, 1285, 1761
TfiI GAWTC 8 cut(s) 311, 1150, 1173, 1230, 1843, 1985, 2245, 2340
Tru1I TTAA 4 cut(s) 210, 471, 1400, 1971
Tru9I TTAA 4 cut(s) 210, 471, 1400, 1971
TscAI CASTG 8 cut(s) 994, 1357, 1487, 1731, 1930, 2012, 2190, 2242
TseFI GTSAC 3 cut(s) 343, 907, 1109
TseI GCWGC 6 cut(s) 122, 125, 350, 2360, 2447, 2450
Tsp45I GTSAC 3 cut(s) 343, 907, 1109
TspDTI ATGAA 9 cut(s) 201, 324, 591, 875, 929, 968, 1457, 1824, 1989
TspGWI ACGGA 8 cut(s) 17, 47, 152, 185, 297, 419, 1682, 2459
TspRI CASTG 8 cut(s) 994, 1357, 1487, 1731, 1930, 2012, 2190, 2242
Van91I CCANNNNNTGG 1 cut(s) 590
VneI GTGCAC 1 cut(s) 372
VpaK11BI GGWCC 7 cut(s) 771, 931, 1070, 1121, 1238, 1913, 1928
XapI RAATTY 3 cut(s) 1057, 1243, 2199
XceI RCATGY 1 cut(s) 1951
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XhoI CTCGAG 1 cut(s) 494
XmiI GTMKAC 3 cut(s) 84, 606, 1944
XmnI GAANNNNTTC 1 cut(s) 1791
XspI CTAG 1 cut(s) 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.