AT3G16230

AKAP7 2'5' RNA ligase-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001993
Physical Location & Seq
Forward (+)
5500054 .. 5503573
3520 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G16230.1

Sequence Viewer

Length: 1350 bp
ATGTTCGCTTACAGGACTCTGTTTAGGATTGACCGTGTATTTGGGTTTACGAGTAGATGTTTTCAATCGAAACCGGAATCTTTTAGTATCCTGAGCTTTAGGGGAAGTGAGAAGAGTGCCATGGATGGATATAAGAAGCAGAAGATGGTAAATTTAGTGTGGAGACCCATCTCCACTCAAACCTCTTCAGTTATTGCTGAAGCTGGAAATGAAGTCCAAGAAGTAGCTCAGTGTAGCAAATCAAGTGATGTTTCTAAGGAAGTTATTGAAGGAGAGACAGCTTCAGTTGTTTCAGCTGGAAAGCATTCAGTTTCTCTTGAGGTTGGGGCTTCTTTGATTAAGTTCATTAGAGGGAAAGAAGGAACGACGCAGATGAAACTTGAAGAGGAAATGGGAGTGAAGATCATTCTTCCATCATCAAGGAACAAAGATCATATCAGCATTGAAGGCGGTTCAGTGGATTGTGTGACTAAAGCTTCAAAAAGAATAGCAACCATTATCGATGAGGTTGTAAGGAGTCCAAGTCTCGACTACTCACACTTTGTATCACTTCCATTAGCCATACATCCTGAGTTAGTGGACAAGCTAGTGAATTTTCAGAATTCTATCCTCGGGATCCATAGCATAGCTAGTGATAAGCAAGACGACCAAGCTAATAGAGCAACCACAAGTGTGGCAGTTGATCTAAAAGCTAATAGTGAAACAAACCAGGTCAATGTGGGTATCAAGAGCATACCAATTGTCAGTTACCCGCCTAAGGCCAAATCAAAGTCATCTACTTTATTGGACTTAGGAATTGAAAAGTCCATATTTATTAAGCCAAGTACTTTTCACTTGACTGTGGTTATGCTGAAGTTATGGAACAAAGATCGTGTCAATGCAGCGTGTGATGTTCTGAAGAGTATATTTCCTAGTGTGATGGATGCTTTGGATAACAAACCAGTATTCATTAGACTTAAAGGATTGGATTGTATGAGAGGACCATTAGACAAGACTCGTGTTCTCTATGCTCCTGTTGAAGAAATTGGTGATGAAGGCCGGCTTTTACGTGCTTGTCAAGTTATTACTGATGCATTTGTGAAAGCTGGGCTTGTTCTCGAGAAAGATGCAAAGCAAAGCTTGAAGCTGCATGTGACCGTGATGAATGCGAGGCACAGGAAAAGGAGAAAGAACAATAAGAAGAAGATGGAGACGTTTGATGCAAGAGAGATACACAAACAGTTTGGAAACGAAGACTGGGGAGAATATCTGATCCAAGAAGCTCATCTCTCACAGCGTTTTGTGTTCGACCAGAACGGTTATTACCGTTGCTGTGGTTCAATACCATTTCCAGGAGAACAGAGAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AKAP7_NLS Auxin_canalis KH_1 PH_2

Protein Analysis

449

Amino Acids

50.08

Weight (kDa)

9.17

Isoelectric Point (pI)

39.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_1 PF00013 104 - 163 2.2e-07 KH domain
AKAP7_NLS PF10469 178 - 443 1.1e-52 AKAP7 2'5' RNA ligase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 450, 752
AclWI GGATC 3 cut(s) 610, 623, 1246
AcsI RAATTY 3 cut(s) 151, 592, 601
AcuI CTGAAG 5 cut(s) 171, 219, 267, 872, 917
AfaI GTAC 1 cut(s) 826
AfiI CCNNNNNNNGG 2 cut(s) 757, 1331
AgsI TTSAA 9 cut(s) 65, 269, 383, 446, 480, 800, 1019, 1123, 1320
AjnI CCWGG 2 cut(s) 708, 1330
AleI CACNNNNGTG 1 cut(s) 671
Alw26I GTCTC 4 cut(s) 157, 269, 530, 1184
AlwI GGATC 3 cut(s) 610, 623, 1246
Ama87I CYCGRG 2 cut(s) 611, 1097
AoxI GGCC 2 cut(s) 759, 1036
ApeKI GCWGC 2 cut(s) 881, 1126
ApoI RAATTY 3 cut(s) 151, 592, 601
ArsI GACNNNNNNTTYG 4 cut(s) 23, 55, 858, 890
Asp700I GAANNNNTTC 1 cut(s) 304
AspS9I GGNCC 1 cut(s) 980
AsuHPI GGTGA 1 cut(s) 1040
AvaI CYCGRG 2 cut(s) 611, 1097
AvaII GGWCC 1 cut(s) 980
AxyI CCTNAGG 1 cut(s) 756
BamHI GGATCC 1 cut(s) 615
BauI CACGAG 1 cut(s) 996
BbsI GAAGAC 1 cut(s) 1239
BbvI GCAGC 2 cut(s) 893, 1113
BccI CCATC 6 cut(s) 119, 139, 176, 421, 913, 1180
BcgI CGANNNNNNTGC 2 cut(s) 1088, 1122
BciT130I CCWGG 2 cut(s) 710, 1332
BciVI GTATCC 1 cut(s) 98
BcoDI GTCTC 4 cut(s) 157, 269, 530, 1184
BfaI CTAG 3 cut(s) 587, 630, 912
BfuI GTATCC 1 cut(s) 98
BisI GCNGC 2 cut(s) 882, 1127
BlsI GCNGC 2 cut(s) 883, 1128
BmcAI AGTACT 1 cut(s) 826
Bme1390I CCNGG 2 cut(s) 710, 1332
Bme18I GGWCC 1 cut(s) 980
BmeT110I CYCGRG 2 cut(s) 611, 1097
BmgT120I GGNCC 1 cut(s) 980
BmiI GGNNCC 1 cut(s) 617
BmrFI CCNGG 2 cut(s) 710, 1332
BmrI ACTGGG 1 cut(s) 1246
BmsI GCATC 4 cut(s) 913, 1060, 1096, 1189
BmuI ACTGGG 1 cut(s) 1246
BpiI GAAGAC 1 cut(s) 1239
Bpu10I CCTNAGC 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 338
Bsa29I ATCGAT 1 cut(s) 501
BsaAI YACGTR 1 cut(s) 1049
BsaI GGTCTC 1 cut(s) 157
BsaJI CCNNGG 2 cut(s) 120, 610
BsaWI WCCGGW 1 cut(s) 73
BsaXI ACNNNNNCTCC 2 cut(s) 508, 538
Bsc4I CCNNNNNNNGG 2 cut(s) 757, 1331
Bse118I RCCGGY 1 cut(s) 1038
Bse1I ACTGG 2 cut(s) 941, 1241
Bse21I CCTNAGG 1 cut(s) 756
BseBI CCWGG 2 cut(s) 710, 1332
BseCI ATCGAT 1 cut(s) 501
BseDI CCNNGG 2 cut(s) 120, 610
BseGI GGATG 3 cut(s) 130, 565, 928
BseLI CCNNNNNNNGG 2 cut(s) 757, 1331
BseMII CTCAG 3 cut(s) 83, 242, 561
BseNI ACTGG 2 cut(s) 941, 1241
BseXI GCAGC 2 cut(s) 893, 1113
BseYI CCCAGC 1 cut(s) 1085
BshFI GGCC 2 cut(s) 761, 1038
BshVI ATCGAT 1 cut(s) 501
BsiHKCI CYCGRG 2 cut(s) 611, 1097
BsiSI CCGG 2 cut(s) 74, 1039
BslI CCNNNNNNNGG 2 cut(s) 757, 1331
BsmAI GTCTC 4 cut(s) 157, 269, 530, 1184
BsmBI CGTCTC 1 cut(s) 1184
BsmI GAATGC 2 cut(s) 304, 1150
BsnI GGCC 2 cut(s) 761, 1038
Bso31I GGTCTC 1 cut(s) 157
BsoBI CYCGRG 2 cut(s) 611, 1097
Bsp143I GATC 6 cut(s) 402, 430, 615, 682, 868, 1251
Bsp19I CCATGG 1 cut(s) 120
BspACI CCGC 2 cut(s) 450, 752
BspANI GGCC 2 cut(s) 761, 1038
BspCNI CTCAG 3 cut(s) 84, 241, 562
BspDI ATCGAT 1 cut(s) 501
BspLI GGNNCC 1 cut(s) 617
BspPI GGATC 3 cut(s) 610, 623, 1246
BspTNI GGTCTC 1 cut(s) 157
BsrFI RCCGGY 1 cut(s) 1038
BsrI ACTGG 2 cut(s) 941, 1241
BssAI RCCGGY 1 cut(s) 1038
BssECI CCNNGG 2 cut(s) 120, 610
BssMI GATC 6 cut(s) 402, 430, 615, 682, 868, 1251
BssSI CACGAG 1 cut(s) 996
BssT1I CCWWGG 1 cut(s) 120
Bst2BI CACGAG 1 cut(s) 996
Bst2UI CCWGG 2 cut(s) 710, 1332
Bst4CI ACNGT 6 cut(s) 35, 841, 1138, 1221, 1298, 1307
Bst6I CTCTTC 4 cut(s) 107, 190, 378, 893
BstBAI YACGTR 1 cut(s) 1049
BstC8I GCNNGC 1 cut(s) 1040
BstDEI CTNAG 6 cut(s) 92, 228, 255, 570, 756, 790
BstDSI CCRYGG 1 cut(s) 120
BstF5I GGATG 3 cut(s) 130, 565, 928
BstKTI GATC 6 cut(s) 405, 433, 618, 685, 871, 1254
BstMAI GTCTC 4 cut(s) 157, 269, 530, 1184
BstMBI GATC 6 cut(s) 402, 430, 615, 682, 868, 1251
BstMWI GCNNNNNNNGC 2 cut(s) 447, 659
BstNI CCWGG 2 cut(s) 710, 1332
BstNSI RCATGY 1 cut(s) 1133
BstSCI CCNGG 2 cut(s) 708, 1330
BstV1I GCAGC 2 cut(s) 893, 1113
BstV2I GAAGAC 1 cut(s) 1239
BstX2I RGATCY 1 cut(s) 615
BstXI CCANNNNNNTGG 1 cut(s) 673
BstYI RGATCY 1 cut(s) 615
Bsu15I ATCGAT 1 cut(s) 501
Bsu36I CCTNAGG 1 cut(s) 756
BsuI GTATCC 1 cut(s) 98
BsuRI GGCC 2 cut(s) 761, 1038
BsuTUI ATCGAT 1 cut(s) 501
BtgI CCRYGG 1 cut(s) 120
BtsCI GGATG 3 cut(s) 130, 565, 928
BtsIMutI CAGTG 2 cut(s) 236, 462
Cac8I GCNNGC 1 cut(s) 1040
Cfr10I RCCGGY 1 cut(s) 1038
Cfr13I GGNCC 1 cut(s) 980
ClaI ATCGAT 1 cut(s) 501
CseI GACGC 1 cut(s) 376
CsiI ACCWGGT 1 cut(s) 708
Csp6I GTAC 1 cut(s) 825
CviAII CATG 2 cut(s) 121, 1130
CviQI GTAC 1 cut(s) 825
DdeI CTNAG 6 cut(s) 92, 228, 255, 570, 756, 790
DpnI GATC 6 cut(s) 404, 432, 617, 684, 870, 1253
DpnII GATC 6 cut(s) 402, 430, 615, 682, 868, 1251
Eam1104I CTCTTC 4 cut(s) 107, 190, 378, 893
EarI CTCTTC 4 cut(s) 107, 190, 378, 893
Eco130I CCWWGG 1 cut(s) 120
Eco31I GGTCTC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 980
Eco57I CTGAAG 5 cut(s) 171, 219, 267, 872, 917
Eco81I CCTNAGG 1 cut(s) 756
Eco88I CYCGRG 2 cut(s) 611, 1097
EcoRI GAATTC 1 cut(s) 601
EcoRII CCWGG 2 cut(s) 708, 1330
EcoT14I CCWWGG 1 cut(s) 120
EcoT22I ATGCAT 1 cut(s) 1075
ErhI CCWWGG 1 cut(s) 120
Esp3I CGTCTC 1 cut(s) 1184
FaeI CATG 2 cut(s) 124, 1133
FalI AAGNNNNNCTT 6 cut(s) 1026, 1058, 1074, 1106, 1103, 1135
FatI CATG 2 cut(s) 120, 1129
FauI CCCGC 1 cut(s) 759
Fnu4HI GCNGC 2 cut(s) 882, 1127
FokI GGATG 3 cut(s) 137, 552, 935
Fsp4HI GCNGC 2 cut(s) 882, 1127
FspBI CTAG 3 cut(s) 587, 630, 912
GluI GCNGC 2 cut(s) 882, 1127
GsaI CCCAGC 1 cut(s) 1089
HaeIII GGCC 2 cut(s) 761, 1038
HapII CCGG 2 cut(s) 74, 1039
HgaI GACGC 1 cut(s) 376
Hin1II CATG 2 cut(s) 124, 1133
HindIII AAGCTT 2 cut(s) 474, 1117
HinfI GANTC 4 cut(s) 16, 77, 517, 994
HpaII CCGG 2 cut(s) 74, 1039
HphI GGTGA 1 cut(s) 1040
Hpy166II GTNNAC 2 cut(s) 48, 580
Hpy188I TCNGA 3 cut(s) 600, 897, 1251
Hpy188III TCNNGA 8 cut(s) 91, 317, 527, 569, 613, 727, 1097, 1099
Hpy8I GTNNAC 2 cut(s) 48, 580
Hpy99I CGWCG 1 cut(s) 370
HpyAV CCTTC 4 cut(s) 263, 353, 440, 1028
HpyCH4III ACNGT 6 cut(s) 35, 841, 1138, 1221, 1298, 1307
HpyCH4IV ACGT 2 cut(s) 1048, 1193
HpyCH4V TGCA 5 cut(s) 881, 1073, 1109, 1129, 1202
HpyF10VI GCNNNNNNNGC 2 cut(s) 447, 659
HpyF3I CTNAG 6 cut(s) 92, 228, 255, 570, 756, 790
HpySE526I ACGT 2 cut(s) 1048, 1193
Hsp92II CATG 2 cut(s) 124, 1133
KroI GCCGGC 1 cut(s) 1038
KroNI GCCGGC 1 cut(s) 1040
Kzo9I GATC 6 cut(s) 402, 430, 615, 682, 868, 1251
LmnI GCTCC 1 cut(s) 1015
Lsp1109I GCAGC 2 cut(s) 893, 1113
LweI GCATC 4 cut(s) 913, 1060, 1096, 1189
MabI ACCWGGT 1 cut(s) 708
MaeI CTAG 3 cut(s) 587, 630, 912
MaeII ACGT 2 cut(s) 1048, 1193
MaeIII GTNAC 3 cut(s) 466, 746, 1132
MalI GATC 6 cut(s) 404, 432, 617, 684, 870, 1253
MboI GATC 6 cut(s) 402, 430, 615, 682, 868, 1251
MfeI CAATTG 1 cut(s) 738
MflI RGATCY 1 cut(s) 615
MluCI AATT 6 cut(s) 151, 592, 601, 738, 795, 1023
MlyI GAGTC 3 cut(s) 10, 526, 988
MnlI CCTC 8 cut(s) 193, 313, 344, 379, 499, 620, 971, 1143
Mph1103I ATGCAT 1 cut(s) 1075
MroNI GCCGGC 1 cut(s) 1038
MroXI GAANNNNTTC 1 cut(s) 304
MseI TTAA 3 cut(s) 339, 816, 957
MslI CAYNNNNRTG 1 cut(s) 671
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 2 cut(s) 74, 1039
MspR9I CCNGG 2 cut(s) 710, 1332
MunI CAATTG 1 cut(s) 738
Mva1269I GAATGC 2 cut(s) 304, 1150
MvaI CCWGG 2 cut(s) 710, 1332
MwoI GCNNNNNNNGC 2 cut(s) 447, 659
NaeI GCCGGC 1 cut(s) 1040
NcoI CCATGG 1 cut(s) 120
NdeII GATC 6 cut(s) 402, 430, 615, 682, 868, 1251
NgoMIV GCCGGC 1 cut(s) 1038
NlaIII CATG 2 cut(s) 124, 1133
NlaIV GGNNCC 1 cut(s) 617
NmuCI GTSAC 2 cut(s) 466, 1132
NsiI ATGCAT 1 cut(s) 1075
NspI RCATGY 1 cut(s) 1133
OliI CACNNNNGTG 1 cut(s) 671
PaeR7I CTCGAG 1 cut(s) 1097
PctI GAATGC 2 cut(s) 304, 1150
PdiI GCCGGC 1 cut(s) 1040
PdmI GAANNNNTTC 1 cut(s) 304
PfeI GAWTC 1 cut(s) 77
PfoI TCCNGGA 1 cut(s) 1330
PkrI GCNGC 2 cut(s) 883, 1128
PleI GAGTC 3 cut(s) 10, 525, 988
PpsI GAGTC 3 cut(s) 10, 525, 988
Ppu21I YACGTR 1 cut(s) 1049
Psp6I CCWGG 2 cut(s) 708, 1330
PspFI CCCAGC 1 cut(s) 1085
PspGI CCWGG 2 cut(s) 708, 1330
PspN4I GGNNCC 1 cut(s) 617
PspPI GGNCC 1 cut(s) 980
PsrI GAACNNNNNNTAC 2 cut(s) 1286, 1318
PsuI RGATCY 1 cut(s) 615
PvuII CAGCTG 1 cut(s) 296
RsaI GTAC 1 cut(s) 826
RsaNI GTAC 1 cut(s) 825
RseI CAYNNNNRTG 1 cut(s) 671
SaqAI TTAA 3 cut(s) 339, 816, 957
SatI GCNGC 2 cut(s) 882, 1127
Sau3AI GATC 6 cut(s) 402, 430, 615, 682, 868, 1251
Sau96I GGNCC 1 cut(s) 980
ScaI AGTACT 1 cut(s) 826
SchI GAGTC 3 cut(s) 10, 526, 988
ScrFI CCNGG 2 cut(s) 710, 1332
SexAI ACCWGGT 1 cut(s) 708
SfaNI GCATC 4 cut(s) 913, 1060, 1096, 1189
Sfr274I CTCGAG 1 cut(s) 1097
SinI GGWCC 1 cut(s) 980
SlaI CTCGAG 1 cut(s) 1097
SmiMI CAYNNNNRTG 1 cut(s) 671
SmlI CTYRAG 2 cut(s) 317, 1097
SmoI CTYRAG 2 cut(s) 317, 1097
Sse9I AATT 6 cut(s) 151, 592, 601, 738, 795, 1023
SsiI CCGC 2 cut(s) 450, 752
SspMI CTAG 3 cut(s) 587, 630, 912
StyD4I CCNGG 2 cut(s) 708, 1330
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 6 cut(s) 35, 841, 1138, 1221, 1298, 1307
TaiI ACGT 2 cut(s) 1051, 1196
TaqI TCGA 5 cut(s) 68, 501, 528, 1098, 1287
TasI AATT 6 cut(s) 151, 592, 601, 738, 795, 1023
TatI WGTACW 1 cut(s) 824
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 3 cut(s) 339, 816, 957
Tru9I TTAA 3 cut(s) 339, 816, 957
TscAI CASTG 2 cut(s) 236, 462
TseFI GTSAC 2 cut(s) 466, 1132
TseI GCWGC 2 cut(s) 881, 1126
Tsp45I GTSAC 2 cut(s) 466, 1132
TspDTI ATGAA 6 cut(s) 225, 334, 389, 937, 1047, 1157
TspRI CASTG 2 cut(s) 236, 462
VpaK11BI GGWCC 1 cut(s) 980
XapI RAATTY 3 cut(s) 151, 592, 601
XceI RCATGY 1 cut(s) 1133
XhoI CTCGAG 1 cut(s) 1097
XmnI GAANNNNTTC 1 cut(s) 304
XspI CTAG 3 cut(s) 587, 630, 912
ZrmI AGTACT 1 cut(s) 826
Zsp2I ATGCAT 1 cut(s) 1075
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.