Rh2DG426800

AKAP7 2'5' RNA ligase-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001993
Physical Location & Seq
Forward (+)
61827793 .. 61828787
995 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG426800.1

Sequence Viewer

Length: 423 bp
ATGGAAGCCTTAGATAATCGCGCTCTATCCATAAGACTCAAGGGACTGAATTGTATGAGAGGTTCTTTTGCCAAAGCTGGTGTTCTATATGCGCCTGTTGAAGAAATTGGCAATGAGGGCCGACTTATGCGTGCCTGTCAAGTCATTATTGATGCATTTGTTAAAGCAGGGCTTGTTCTAGAGAAAGATGCAAATCAGAAATTAAAGCTCCATGCAACGGTGATGAAGGCAAGGCATGGAGAAAGGAATCAAAAGAGAAAATTTAGTACCTTTGATGCCCGTGGCATATATAAGCAGTATGGATCCGAGGAATGGGGAGAGTATCTTATCCGTGAAGCTCATCTTTCACAAAGGTTTGTGTACGATGACAAGGGATATTATCATTGTTGTGCTTCTATACCTTTTCCAGAAATATGCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AKAP7_NLS Auxin_canalis KH_1 PH_2

Protein Analysis

140

Amino Acids

15.99

Weight (kDa)

9.11

Isoelectric Point (pI)

28.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AKAP7_NLS PF10469 3 - 135 2.9e-32 AKAP7 2'5' RNA ligase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 21
AclWI GGATC 2 cut(s) 297, 310
AcsI RAATTY 1 cut(s) 260
AfaI GTAC 2 cut(s) 268, 362
AfiI CCNNNNNNNGG 2 cut(s) 217, 312
AgsI TTSAA 1 cut(s) 101
AluBI AGCT 3 cut(s) 77, 208, 338
AluI AGCT 3 cut(s) 77, 208, 338
AlwI GGATC 2 cut(s) 297, 310
AoxI GGCC 1 cut(s) 118
ApoI RAATTY 1 cut(s) 260
AspLEI GCGC 2 cut(s) 23, 94
AspS9I GGNCC 1 cut(s) 118
AsuHPI GGTGA 1 cut(s) 232
BamHI GGATCC 1 cut(s) 302
BfaI CTAG 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 118
BmiI GGNNCC 1 cut(s) 304
BmsI GCATC 3 cut(s) 142, 178, 265
BpuEI CTTGAG 1 cut(s) 23
BsaBI GATNNNNATC 1 cut(s) 192
BsaJI CCNNGG 2 cut(s) 280, 306
Bsc4I CCNNNNNNNGG 2 cut(s) 217, 312
Bse3DI GCAATG 1 cut(s) 118
Bse8I GATNNNNATC 1 cut(s) 192
BseDI CCNNGG 2 cut(s) 280, 306
BseJI GATNNNNATC 1 cut(s) 192
BseLI CCNNNNNNNGG 2 cut(s) 217, 312
BseMI GCAATG 1 cut(s) 118
Bsh1236I CGCG 1 cut(s) 21
BshFI GGCC 1 cut(s) 120
BslFI GGGAC 1 cut(s) 57
BslI CCNNNNNNNGG 2 cut(s) 217, 312
BsmFI GGGAC 1 cut(s) 57
BsnI GGCC 1 cut(s) 120
Bsp143I GATC 1 cut(s) 302
BspANI GGCC 1 cut(s) 120
BspFNI CGCG 1 cut(s) 21
BspLI GGNNCC 1 cut(s) 304
BspPI GGATC 2 cut(s) 297, 310
BsrDI GCAATG 1 cut(s) 118
BssECI CCNNGG 2 cut(s) 280, 306
BssMI GATC 1 cut(s) 302
Bst4CI ACNGT 1 cut(s) 220
BstC8I GCNNGC 1 cut(s) 132
BstDEI CTNAG 1 cut(s) 10
BstDSI CCRYGG 1 cut(s) 280
BstFNI CGCG 1 cut(s) 21
BstHHI GCGC 2 cut(s) 23, 94
BstKTI GATC 1 cut(s) 305
BstMBI GATC 1 cut(s) 302
BstMWI GCNNNNNNNGC 1 cut(s) 117
BstUI CGCG 1 cut(s) 21
BstX2I RGATCY 1 cut(s) 302
BstYI RGATCY 1 cut(s) 302
BsuRI GGCC 1 cut(s) 120
BtgI CCRYGG 1 cut(s) 280
Cac8I GCNNGC 1 cut(s) 132
CfoI GCGC 2 cut(s) 23, 94
Cfr13I GGNCC 1 cut(s) 118
Csp6I GTAC 2 cut(s) 267, 361
CviAII CATG 2 cut(s) 212, 236
CviJI RGCY 6 cut(s) 8, 77, 120, 172, 208, 338
CviKI_1 RGCY 6 cut(s) 8, 77, 120, 172, 208, 338
CviQI GTAC 2 cut(s) 267, 361
DdeI CTNAG 1 cut(s) 10
DpnI GATC 1 cut(s) 304
DpnII GATC 1 cut(s) 302
EcoT22I ATGCAT 1 cut(s) 157
FaeI CATG 2 cut(s) 215, 239
FalI AAGNNNNNCTT 4 cut(s) 156, 188, 327, 359
FaqI GGGAC 1 cut(s) 57
FatI CATG 2 cut(s) 211, 235
FspBI CTAG 1 cut(s) 179
GlaI GCGC 2 cut(s) 22, 93
HaeIII GGCC 1 cut(s) 120
HhaI GCGC 2 cut(s) 23, 94
Hin1II CATG 2 cut(s) 215, 239
Hin6I GCGC 2 cut(s) 21, 92
HinP1I GCGC 2 cut(s) 21, 92
HinfI GANTC 2 cut(s) 36, 247
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 1 cut(s) 361
Hpy188I TCNGA 2 cut(s) 198, 307
Hpy188III TCNNGA 2 cut(s) 179, 407
Hpy8I GTNNAC 1 cut(s) 361
HpyAV CCTTC 1 cut(s) 220
HpyCH4III ACNGT 1 cut(s) 220
HpyCH4V TGCA 4 cut(s) 155, 191, 215, 417
HpyF10VI GCNNNNNNNGC 1 cut(s) 117
HpyF3I CTNAG 1 cut(s) 10
Hsp92II CATG 2 cut(s) 215, 239
HspAI GCGC 2 cut(s) 21, 92
Kzo9I GATC 1 cut(s) 302
LmnI GCTCC 1 cut(s) 213
LpnPI CCDG 4 cut(s) 63, 108, 148, 153
LweI GCATC 3 cut(s) 142, 178, 265
MaeI CTAG 1 cut(s) 179
MalI GATC 1 cut(s) 304
MboI GATC 1 cut(s) 302
MboII GAAGA 1 cut(s) 113
MflI RGATCY 1 cut(s) 302
MluCI AATT 4 cut(s) 49, 105, 200, 260
MlyI GAGTC 1 cut(s) 30
MnlI CCTC 3 cut(s) 53, 109, 301
Mph1103I ATGCAT 1 cut(s) 157
MseI TTAA 2 cut(s) 162, 203
MslI CAYNNNNRTG 1 cut(s) 387
MvnI CGCG 1 cut(s) 21
MwoI GCNNNNNNNGC 1 cut(s) 117
NdeII GATC 1 cut(s) 302
NlaIII CATG 2 cut(s) 215, 239
NlaIV GGNNCC 1 cut(s) 304
NsiI ATGCAT 1 cut(s) 157
PfeI GAWTC 1 cut(s) 247
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
PspN4I GGNNCC 1 cut(s) 304
PspPI GGNCC 1 cut(s) 118
PsrI GAACNNNNNNTAC 2 cut(s) 46, 78
PsuI RGATCY 1 cut(s) 302
RsaI GTAC 2 cut(s) 268, 362
RsaNI GTAC 2 cut(s) 267, 361
RseI CAYNNNNRTG 1 cut(s) 387
SaqAI TTAA 2 cut(s) 162, 203
Sau3AI GATC 1 cut(s) 302
Sau96I GGNCC 1 cut(s) 118
SchI GAGTC 1 cut(s) 30
SetI ASST 7 cut(s) 64, 79, 210, 272, 340, 356, 403
SfaNI GCATC 3 cut(s) 142, 178, 265
SmiMI CAYNNNNRTG 1 cut(s) 387
SmlI CTYRAG 1 cut(s) 38
SmoI CTYRAG 1 cut(s) 38
Sse9I AATT 4 cut(s) 49, 105, 200, 260
SspMI CTAG 1 cut(s) 179
TaaI ACNGT 1 cut(s) 220
TasI AATT 4 cut(s) 49, 105, 200, 260
TfiI GAWTC 1 cut(s) 247
Tru1I TTAA 2 cut(s) 162, 203
Tru9I TTAA 2 cut(s) 162, 203
TspDTI ATGAA 1 cut(s) 239
TspGWI ACGGA 1 cut(s) 320
XapI RAATTY 1 cut(s) 260
XbaI TCTAGA 1 cut(s) 178
XspI CTAG 1 cut(s) 179
Zsp2I ATGCAT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.