Rw2G032990

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001993
Physical Location & Seq
Forward (+)
54099243 .. 54101553
2311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G032990.1

Sequence Viewer

Length: 1254 bp
ATGGCCTCACAAGACTACTCTCCCCCAACTCCATCTTTTGCAACAAATAAAAACAAGTTCTTCTCCGGCTTCACCAGAAGCACATCAACCAAAGAACCATCCTTCTCACAATTTCCTTATACACCGCCTCGCCATAACACCAATGACGTCTCTCCTTCCTTCTCCCAAACCGAAAGCCTCCACGCCTCCCCAACCTCCTCCCCTCAGTTCATCCCTCTCAAACACCCCGACCTCCTCACCGCCTTCCGTTGCATCTCCTCCGTCCTGAAAAAGGACGGCCGGATCTTGTCCATCACCGCCTCCAGTGGCCTAGTTTACTCCGGCTCAGATACCAATGTCATTAGGGTTTGGAAGCTACCCGAGTTCACAGAATGTGGGCAGCTCAAGACCAAAGCTTGCATGGTGGTGGCACTAGAGGTGTCTCATGATAGGGTTTATGCTGCTTATGGTGATGGGAAGGTGAGAATATGGCAGAGAACATGGGATGGAGGGTTAAAGCATGAGAGGTTGGCTACGATTCCGAAGATCGGAAGTTATGTCCGGAGTTTCTACATTGCCGGGAAAGATAAGACGCATATGGGTGCCATAACATCACTAGCCATCAATGTAGCAGAGGACATCCTCTACTCAGCTTCCATTGACAAAACAGTGAAAGTATGGCGAATTTCAGACCTCAAATGCATCGAGACAATCCAAGCCCACGCCGAGCCAATCAATGCCATCATTGCCGCGGACGATGGAATCCTCTACACGGCCTCCGACGATGCAACTGTTCGAGTCTGGCGCCGCAACTTTTGTCGTGGCGACCAGCTTCCACATTCACTCACCGTGACTCTACCGGCCAAGTACTCCCCCGTCAAGACCCTAACCCTAACAGCGGATGGTGCAATCTTGTACGGGGGTTGCACTGACGGGTACATACACTTTTGGCTAGGGGGCCACACCCATGCAGTTATGTGCTTGGCCAGCGTGGCGAACTACGTGGTCAGTGGGTCAGCTGACTCAACGAGTAGGATTTGGTCTAAGGACCCTGATGGTCTGCATACTTGTCTAGCAGTCCTTGTGGGTCACAGAGGGCCAATCAGGTGTGTGACAGCATTTCTGGGAAGGCTGGGAGAAGAGACTGAGGAGGGGTGCACAATCTGTACAGGGAGTCTTGATGGTGTTCTCAAAGTGTGGCGTGTGACATCCAAAAGTAATAGTGGTAATAGTGGGTGTTTAGCACAGGATGGGAGTGAGTATTTCGAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

417

Amino Acids

45.45

Weight (kDa)

8.19

Isoelectric Point (pI)

37.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EML PF23409 107 - 300 5.4e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 180 - 261 1.2e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5 PF25175 190 - 310 3.1e-16 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 190 - 262 1.9e-12 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 190 - 365 2.1e-15 THOC3 beta-propeller domain
WDR55 PF24796 190 - 402 6.4e-09 WDR55
WD40_Prp19 PF24814 191 - 310 5.4e-12 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 191 - 263 7.9e-08 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 191 - 262 3.9e-07 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 197 - 262 2.2e-06 WDHD1 first WD40 domain
Beta-prop_TEP1_2nd PF25047 203 - 310 3.9e-06 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 293 - 398 1.3e-07 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 311 - 397 9e-09 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccB1I GGYRCC 2 cut(s) 581, 783
AccII CGCG 1 cut(s) 731
AccIII TCCGGA 1 cut(s) 540
AciI CCGC 7 cut(s) 125, 240, 297, 729, 731, 787, 878
AclWI GGATC 1 cut(s) 290
AcoI YGGCCR 3 cut(s) 277, 840, 963
AcsI RAATTY 1 cut(s) 663
AcyI GRCGYC 2 cut(s) 147, 784
AfaI GTAC 4 cut(s) 848, 896, 917, 1147
AfiI CCNNNNNNNGG 4 cut(s) 271, 527, 751, 877
AluBI AGCT 7 cut(s) 355, 382, 395, 632, 811, 998, 1249
AluI AGCT 7 cut(s) 355, 382, 395, 632, 811, 998, 1249
Alw21I GWGCWC 1 cut(s) 1139
Alw26I GTCTC 4 cut(s) 154, 426, 680, 1115
Alw44I GTGCAC 1 cut(s) 1135
AlwI GGATC 1 cut(s) 290
Ama87I CYCGRG 1 cut(s) 359
Aor13HI TCCGGA 1 cut(s) 540
AoxI GGCC 8 cut(s) 3, 277, 307, 753, 840, 937, 963, 1076
ApaLI GTGCAC 1 cut(s) 1135
ApeKI GCWGC 2 cut(s) 379, 440
ApoI RAATTY 1 cut(s) 663
AspLEI GCGC 1 cut(s) 786
AspS9I GGNCC 3 cut(s) 937, 1027, 1076
AsuC2I CCSGG 1 cut(s) 559
AsuHPI GGTGA 6 cut(s) 64, 229, 286, 461, 472, 817
AvaI CYCGRG 1 cut(s) 359
AvaII GGWCC 1 cut(s) 1027
BaeGI GKGCMC 1 cut(s) 1139
BaeI ACNNNNGTAYC 2 cut(s) 321, 354
BalI TGGCCA 1 cut(s) 965
BanI GGYRCC 2 cut(s) 581, 783
Bbv12I GWGCWC 1 cut(s) 1139
BbvI GCAGC 2 cut(s) 391, 427
BceAI ACGGC 2 cut(s) 292, 768
BcnI CCSGG 1 cut(s) 559
BcoDI GTCTC 4 cut(s) 154, 426, 680, 1115
BfaI CTAG 5 cut(s) 311, 413, 596, 932, 1052
BfmI CTRYAG 1 cut(s) 1250
BfoI RGCGCY 1 cut(s) 787
BglI GCCNNNNNGGC 1 cut(s) 971
BisI GCNGC 4 cut(s) 380, 441, 729, 787
BlsI GCNGC 4 cut(s) 381, 442, 730, 788
BmcAI AGTACT 1 cut(s) 848
Bme1390I CCNGG 1 cut(s) 559
Bme18I GGWCC 1 cut(s) 1027
BmeT110I CYCGRG 1 cut(s) 359
BmgT120I GGNCC 3 cut(s) 937, 1027, 1076
BmiI GGNNCC 4 cut(s) 583, 785, 938, 1029
BmrFI CCNGG 1 cut(s) 559
BmsI GCATC 3 cut(s) 261, 690, 754
BpmI CTGGAG 1 cut(s) 286
BpuEI CTTGAG 1 cut(s) 368
BpuMI CCSGG 1 cut(s) 559
BsaAI YACGTR 1 cut(s) 982
BsaHI GRCGYC 2 cut(s) 147, 784
BsaJI CCNNGG 1 cut(s) 729
BsaWI WCCGGW 1 cut(s) 540
BsaXI ACNNNNNCTCC 2 cut(s) 740, 770
Bsc4I CCNNNNNNNGG 4 cut(s) 271, 527, 751, 877
Bse118I RCCGGY 1 cut(s) 838
Bse1I ACTGG 1 cut(s) 303
Bse3DI GCAATG 2 cut(s) 552, 723
BseAI TCCGGA 1 cut(s) 540
BseDI CCNNGG 1 cut(s) 729
BseGI GGATG 7 cut(s) 98, 210, 490, 618, 886, 1187, 1234
BseLI CCNNNNNNNGG 4 cut(s) 271, 527, 751, 877
BseMI GCAATG 2 cut(s) 552, 723
BseMII CTCAG 4 cut(s) 218, 339, 642, 1116
BseNI ACTGG 1 cut(s) 303
BseRI GAGGAG 4 cut(s) 187, 224, 247, 1142
BseSI GKGCMC 1 cut(s) 1139
BseX3I CGGCCG 1 cut(s) 277
BseXI GCAGC 2 cut(s) 391, 427
BseYI CCCAGC 1 cut(s) 1111
Bsh1236I CGCG 1 cut(s) 731
Bsh1285I CGRYCG 1 cut(s) 280
BshFI GGCC 8 cut(s) 5, 279, 309, 755, 842, 939, 965, 1078
BshNI GGYRCC 2 cut(s) 581, 783
BsiEI CGRYCG 1 cut(s) 280
BsiHKAI GWGCWC 1 cut(s) 1139
BsiHKCI CYCGRG 1 cut(s) 359
BsiSI CCGG 6 cut(s) 66, 280, 321, 541, 558, 839
BslI CCNNNNNNNGG 4 cut(s) 271, 527, 751, 877
BsmAI GTCTC 4 cut(s) 154, 426, 680, 1115
BsmBI CGTCTC 1 cut(s) 154
BsnI GGCC 8 cut(s) 5, 279, 309, 755, 842, 939, 965, 1078
BsoBI CYCGRG 1 cut(s) 359
Bsp1286I GDGCHC 1 cut(s) 1139
Bsp13I TCCGGA 1 cut(s) 540
Bsp1407I TGTACA 1 cut(s) 1145
Bsp143I GATC 2 cut(s) 282, 525
BspACI CCGC 7 cut(s) 125, 240, 297, 729, 731, 787, 878
BspANI GGCC 8 cut(s) 5, 279, 309, 755, 842, 939, 965, 1078
BspCNI CTCAG 4 cut(s) 217, 338, 641, 1117
BspEI TCCGGA 1 cut(s) 540
BspFNI CGCG 1 cut(s) 731
BspHI TCATGA 1 cut(s) 424
BspLI GGNNCC 4 cut(s) 583, 785, 938, 1029
BspPI GGATC 1 cut(s) 290
BspT107I GGYRCC 2 cut(s) 581, 783
BsrDI GCAATG 2 cut(s) 552, 723
BsrFI RCCGGY 1 cut(s) 838
BsrGI TGTACA 1 cut(s) 1145
BsrI ACTGG 1 cut(s) 303
BssAI RCCGGY 1 cut(s) 838
BssECI CCNNGG 1 cut(s) 729
BssMI GATC 2 cut(s) 282, 525
BssNI GRCGYC 2 cut(s) 147, 784
Bst4CI ACNGT 3 cut(s) 649, 772, 829
Bst6I CTCTTC 1 cut(s) 1113
BstACI GRCGYC 2 cut(s) 147, 784
BstAUI TGTACA 1 cut(s) 1145
BstBAI YACGTR 1 cut(s) 982
BstC8I GCNNGC 2 cut(s) 397, 967
BstDEI CTNAG 5 cut(s) 204, 325, 628, 1023, 1125
BstDSI CCRYGG 1 cut(s) 729
BstENI CCTNNNNNAGG 1 cut(s) 269
BstF5I GGATG 7 cut(s) 98, 210, 490, 618, 886, 1187, 1234
BstFNI CGCG 1 cut(s) 731
BstH2I RGCGCY 1 cut(s) 787
BstHHI GCGC 1 cut(s) 786
BstKTI GATC 2 cut(s) 285, 528
BstMAI GTCTC 4 cut(s) 154, 426, 680, 1115
BstMBI GATC 2 cut(s) 282, 525
BstMCI CGRYCG 1 cut(s) 280
BstMWI GCNNNNNNNGC 4 cut(s) 725, 884, 966, 971
BstSCI CCNGG 1 cut(s) 557
BstSFI CTRYAG 1 cut(s) 1250
BstSLI GKGCMC 1 cut(s) 1139
BstUI CGCG 1 cut(s) 731
BstV1I GCAGC 2 cut(s) 391, 427
BstX2I RGATCY 1 cut(s) 282
BstYI RGATCY 1 cut(s) 282
BstZI CGGCCG 1 cut(s) 277
BsuRI GGCC 8 cut(s) 5, 279, 309, 755, 842, 939, 965, 1078
BtgI CCRYGG 1 cut(s) 729
BtsCI GGATG 7 cut(s) 98, 210, 490, 618, 886, 1187, 1234
BtsIMutI CAGTG 4 cut(s) 310, 654, 906, 994
Cac8I GCNNGC 2 cut(s) 397, 967
CciI TCATGA 1 cut(s) 424
CfoI GCGC 1 cut(s) 786
Cfr10I RCCGGY 1 cut(s) 838
Cfr13I GGNCC 3 cut(s) 937, 1027, 1076
Cfr42I CCGCGG 1 cut(s) 732
CseI GACGC 1 cut(s) 580
Csp6I GTAC 4 cut(s) 847, 895, 916, 1146
CviAII CATG 5 cut(s) 400, 425, 480, 500, 947
CviQI GTAC 4 cut(s) 847, 895, 916, 1146
DdeI CTNAG 5 cut(s) 204, 325, 628, 1023, 1125
DinI GGCGCC 1 cut(s) 785
DpnI GATC 2 cut(s) 284, 527
DpnII GATC 2 cut(s) 282, 525
EaeI YGGCCR 3 cut(s) 277, 840, 963
EagI CGGCCG 1 cut(s) 277
Eam1104I CTCTTC 1 cut(s) 1113
EarI CTCTTC 1 cut(s) 1113
EclXI CGGCCG 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 1027
Eco52I CGGCCG 1 cut(s) 277
Eco88I CYCGRG 1 cut(s) 359
EcoNI CCTNNNNNAGG 1 cut(s) 269
EcoO109I RGGNCCY 1 cut(s) 1027
EcoT22I ATGCAT 1 cut(s) 683
EgeI GGCGCC 1 cut(s) 785
EheI GGCGCC 1 cut(s) 785
Esp3I CGTCTC 1 cut(s) 154
FaeI CATG 5 cut(s) 403, 428, 483, 503, 950
FatI CATG 5 cut(s) 399, 424, 479, 499, 946
FauNDI CATATG 1 cut(s) 576
Fnu4HI GCNGC 4 cut(s) 380, 441, 729, 787
FokI GGATG 7 cut(s) 85, 197, 497, 605, 893, 1174, 1241
Fsp4HI GCNGC 4 cut(s) 380, 441, 729, 787
FspBI CTAG 5 cut(s) 311, 413, 596, 932, 1052
GlaI GCGC 1 cut(s) 785
GluI GCNGC 4 cut(s) 380, 441, 729, 787
GsaI CCCAGC 1 cut(s) 1115
GsuI CTGGAG 1 cut(s) 286
HaeII RGCGCY 1 cut(s) 787
HaeIII GGCC 8 cut(s) 5, 279, 309, 755, 842, 939, 965, 1078
HapII CCGG 6 cut(s) 66, 280, 321, 541, 558, 839
HgaI GACGC 1 cut(s) 580
HhaI GCGC 1 cut(s) 786
Hin1I GRCGYC 2 cut(s) 147, 784
Hin1II CATG 5 cut(s) 403, 428, 483, 503, 950
Hin6I GCGC 1 cut(s) 784
HinP1I GCGC 1 cut(s) 784
HindIII AAGCTT 1 cut(s) 393
HinfI GANTC 6 cut(s) 517, 741, 777, 832, 1001, 1153
HpaII CCGG 6 cut(s) 66, 280, 321, 541, 558, 839
HphI GGTGA 6 cut(s) 64, 229, 286, 461, 472, 817
Hpy166II GTNNAC 3 cut(s) 316, 366, 1137
Hpy188I TCNGA 5 cut(s) 328, 522, 530, 670, 760
Hpy188III TCNNGA 7 cut(s) 265, 385, 425, 541, 685, 859, 1157
Hpy8I GTNNAC 3 cut(s) 316, 366, 1137
Hpy99I CGWCG 1 cut(s) 764
HpyAV CCTTC 6 cut(s) 112, 165, 169, 253, 451, 1101
HpyCH4III ACNGT 3 cut(s) 649, 772, 829
HpyCH4IV ACGT 2 cut(s) 147, 981
HpyF10VI GCNNNNNNNGC 4 cut(s) 725, 884, 966, 971
HpyF3I CTNAG 5 cut(s) 204, 325, 628, 1023, 1125
HpySE526I ACGT 2 cut(s) 147, 981
Hsp92I GRCGYC 2 cut(s) 147, 784
Hsp92II CATG 5 cut(s) 403, 428, 483, 503, 950
HspAI GCGC 1 cut(s) 784
KasI GGCGCC 1 cut(s) 783
Kpn2I TCCGGA 1 cut(s) 540
KspI CCGCGG 1 cut(s) 732
Kzo9I GATC 2 cut(s) 282, 525
Lsp1109I GCAGC 2 cut(s) 391, 427
LweI GCATC 3 cut(s) 261, 690, 754
MaeI CTAG 5 cut(s) 311, 413, 596, 932, 1052
MaeII ACGT 2 cut(s) 147, 981
MaeIII GTNAC 4 cut(s) 829, 1067, 1090, 1183
MalI GATC 2 cut(s) 284, 527
MboI GATC 2 cut(s) 282, 525
MboII GAAGA 3 cut(s) 52, 535, 1130
MflI RGATCY 1 cut(s) 282
MhlI GDGCHC 1 cut(s) 1139
MlsI TGGCCA 1 cut(s) 965
MluCI AATT 2 cut(s) 110, 663
MluNI TGGCCA 1 cut(s) 965
Mly113I GGCGCC 1 cut(s) 784
MlyI GAGTC 4 cut(s) 786, 826, 995, 1162
MmeI TCCRAC 1 cut(s) 783
Mox20I TGGCCA 1 cut(s) 965
Mph1103I ATGCAT 1 cut(s) 683
MroI TCCGGA 1 cut(s) 540
MscI TGGCCA 1 cut(s) 965
MseI TTAA 1 cut(s) 494
MslI CAYNNNNRTG 3 cut(s) 404, 579, 945
Msp20I TGGCCA 1 cut(s) 965
MspA1I CMGCKG 3 cut(s) 731, 878, 998
MspI CCGG 6 cut(s) 66, 280, 321, 541, 558, 839
MspR9I CCNGG 1 cut(s) 559
MvnI CGCG 1 cut(s) 731
MwoI GCNNNNNNNGC 4 cut(s) 725, 884, 966, 971
NarI GGCGCC 1 cut(s) 784
NciI CCSGG 1 cut(s) 559
NdeI CATATG 1 cut(s) 576
NdeII GATC 2 cut(s) 282, 525
NlaIII CATG 5 cut(s) 403, 428, 483, 503, 950
NlaIV GGNNCC 4 cut(s) 583, 785, 938, 1029
NmeAIII GCCGAG 1 cut(s) 730
NmuCI GTSAC 4 cut(s) 829, 1067, 1090, 1183
NsiI ATGCAT 1 cut(s) 683
PagI TCATGA 1 cut(s) 424
PfeI GAWTC 2 cut(s) 517, 741
PkrI GCNGC 4 cut(s) 381, 442, 730, 788
PleI GAGTC 4 cut(s) 785, 826, 995, 1161
PluTI GGCGCC 1 cut(s) 787
PpsI GAGTC 4 cut(s) 785, 826, 995, 1161
Ppu21I YACGTR 1 cut(s) 982
PpuMI RGGWCCY 1 cut(s) 1027
Psp5II RGGWCCY 1 cut(s) 1027
PspFI CCCAGC 1 cut(s) 1111
PspN4I GGNNCC 4 cut(s) 583, 785, 938, 1029
PspPI GGNCC 3 cut(s) 937, 1027, 1076
PspPPI RGGWCCY 1 cut(s) 1027
PsuI RGATCY 1 cut(s) 282
PvuII CAGCTG 1 cut(s) 998
RsaI GTAC 4 cut(s) 848, 896, 917, 1147
RsaNI GTAC 4 cut(s) 847, 895, 916, 1146
RseI CAYNNNNRTG 3 cut(s) 404, 579, 945
SacII CCGCGG 1 cut(s) 732
SaqAI TTAA 1 cut(s) 494
SatI GCNGC 4 cut(s) 380, 441, 729, 787
Sau3AI GATC 2 cut(s) 282, 525
Sau96I GGNCC 3 cut(s) 937, 1027, 1076
ScaI AGTACT 1 cut(s) 848
SchI GAGTC 4 cut(s) 786, 826, 995, 1162
ScrFI CCNGG 1 cut(s) 559
SduI GDGCHC 1 cut(s) 1139
SfaNI GCATC 3 cut(s) 261, 690, 754
SfcI CTRYAG 1 cut(s) 1250
SfoI GGCGCC 1 cut(s) 785
Sfr303I CCGCGG 1 cut(s) 732
SgrBI CCGCGG 1 cut(s) 732
SinI GGWCC 1 cut(s) 1027
SmiMI CAYNNNNRTG 3 cut(s) 404, 579, 945
SmlI CTYRAG 1 cut(s) 383
SmoI CTYRAG 1 cut(s) 383
Sse9I AATT 2 cut(s) 110, 663
SsiI CCGC 7 cut(s) 125, 240, 297, 729, 731, 787, 878
SspDI GGCGCC 1 cut(s) 783
SspMI CTAG 5 cut(s) 311, 413, 596, 932, 1052
StyD4I CCNGG 1 cut(s) 557
TaaI ACNGT 3 cut(s) 649, 772, 829
TaiI ACGT 2 cut(s) 150, 984
TaqI TCGA 3 cut(s) 684, 775, 1245
TasI AATT 2 cut(s) 110, 663
TatI WGTACW 2 cut(s) 846, 1145
TauI GCSGC 2 cut(s) 731, 789
TfiI GAWTC 2 cut(s) 517, 741
Tru1I TTAA 1 cut(s) 494
Tru9I TTAA 1 cut(s) 494
TscAI CASTG 4 cut(s) 310, 654, 913, 994
TseFI GTSAC 4 cut(s) 829, 1067, 1090, 1183
TseI GCWGC 2 cut(s) 379, 440
Tsp45I GTSAC 4 cut(s) 829, 1067, 1090, 1183
TspDTI ATGAA 1 cut(s) 199
TspGWI ACGGA 2 cut(s) 236, 250
TspRI CASTG 4 cut(s) 310, 654, 913, 994
VneI GTGCAC 1 cut(s) 1135
VpaK11BI GGWCC 1 cut(s) 1027
XagI CCTNNNNNAGG 1 cut(s) 269
XapI RAATTY 1 cut(s) 663
XcmI CCANNNNNNNNNTGG 1 cut(s) 397
XspI CTAG 5 cut(s) 311, 413, 596, 932, 1052
ZraI GACGTC 1 cut(s) 148
ZrmI AGTACT 1 cut(s) 848
Zsp2I ATGCAT 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.