FvH4_6g31950

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001993
Physical Location & Seq
Forward (+)
25059575 .. 25061812
2238 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g31950.t1

Sequence Viewer

Length: 1323 bp
ATGGCCTCACAGGACTACTCTCCCAACTCTTCCCCAATAACCCCATCTTTTGCAACTGACCAAAACAAGTTCTTCACTGGCTTCACCAGAAGCATTTCAACCAAAGAACCCTCTTTCTCACAATTTCCTTACACCCCACCTCGACGTAGCAACAATGACGTCTCTCCTTCCTTCTCCCAAACCCAAAGCCTCCATGCTTCCCCGACCTCCTCCCCTCGCTTCATCCCTCTCAAACACCCCGACCACCTCACCGCCTACCGTTGCATCTCCTCCGTCCTCAAAAAAGACGGGCAGATCTTGTCCATTGCCGCCTCCAATGGCCTAGTTTACTCCGGCTCCGATACCAATGTCATTAGGGTTTGGAAGCTACCCGAGTTCACAGAATGTGGGCAGCTCAAGACCAAAGCTTGCATGGTGGTGGCATTGGAGGTGTCTCATGATAGGGTTTATGCTGCTTATGGTGACGGGAAGGTGAGAATATGGCAGAGAATATGGGACGGTGGATTGAAGCATGAGAGGTTGGCTACCATTCCCAAACTGGGAAGTTATGTCCGGAGTTTTTACACTGCCGGGAAAGATAAGACGATGAAGCATATGGGTGCCATAACATCACTAGCCATCAATGTAGCAGAGGACATCCTCTACTCAGCTTCCATCGACAAGACAGTAAAAGTATGGCGAATCTCAGACCTCAAATGCATTGAAACAATCCAAGCTCACGCGGAGCCGATCAACGCCATCATTGCTGCGGACGATGGTATCCTCTACACAGCCTCGGACGATGCAACGATCCGAGTCTGGCGCCGCAACGTTTGTCGCGGCGGCGACCAGTTTCCACATTCACTCACTGTGACTCTACCGGCTAAGTACTCCCCCGTCAAGACCCTAACCCTAACAGCGGACGGTGCAATCTTGTACGGGGGCTGCACTGACGGGTACATACACTTTTGGCTTAGGGGTTGGTTCTCTGGCCAGGTGCAGTACGGCGGTGCACTCCAGGGTCATACTCATGCAGTGATGTGCTTGGCCAGTGTGGCCAACTATGTGATCAGTGGATCAGCTGACTCGACGAGTAGGATTTGGTCTAAGGACTCTGATGGTCTGCATACTTGTGTAGCAGTCCTTGTGGGTCACAGAGGGCCAATCAGGTGTGTGACAGCATTTCTGGGGAGGCTAGGAGAAGAGACTGAGGATGGTTGCACAATCTGTACTGGGAGTCTTGATGGTGTTCTCAAAGTGTGGCGTGTGACATCCAAAAATAATAGTGGTACTAGTACTGCAGGGTGTTTAGCTCAAGGTGGGAGTGACTATTTTGAGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

441

Amino Acids

47.9

Weight (kDa)

8.62

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EML PF23409 111 - 307 3.8e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 185 - 267 9.1e-07 WDR36/Utp21 second beta-propeller domain
Beta-prop_THOC3 PF25174 195 - 419 4.6e-20 THOC3 beta-propeller domain
WDR55 PF24796 195 - 423 2.6e-10 WDR55
Beta-prop_WDR3_2nd PF25172 196 - 269 3.2e-08 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 196 - 361 8.3e-11 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 197 - 317 5.3e-16 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 197 - 268 2.8e-12 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 198 - 317 1.9e-11 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 204 - 362 2.6e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 284 - 421 1.6e-10 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 303 - 420 1.1e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 330 - 420 2.3e-10 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 162
AccB1I GGYRCC 2 cut(s) 599, 801
AccII CGCG 2 cut(s) 722, 819
AccIII TCCGGA 1 cut(s) 552
AciI CCGC 9 cut(s) 252, 309, 722, 749, 805, 819, 822, 899, 987
AclI AACGTT 1 cut(s) 810
AclWI GGATC 2 cut(s) 784, 1063
AcoI YGGCCR 3 cut(s) 970, 1026, 1035
AcyI GRCGYC 2 cut(s) 159, 802
AfaI GTAC 7 cut(s) 869, 917, 938, 983, 1210, 1270, 1276
AfiI CCNNNNNNNGG 2 cut(s) 539, 898
AgsI TTSAA 3 cut(s) 99, 508, 704
AhlI ACTAGT 1 cut(s) 1271
AjnI CCWGG 2 cut(s) 972, 996
AluBI AGCT 8 cut(s) 367, 394, 407, 650, 716, 1061, 1292, 1318
AluI AGCT 8 cut(s) 367, 394, 407, 650, 716, 1061, 1292, 1318
Alw21I GWGCWC 1 cut(s) 994
Alw26I GTCTC 3 cut(s) 166, 438, 1178
Alw44I GTGCAC 1 cut(s) 990
AlwI GGATC 2 cut(s) 784, 1063
Ama87I CYCGRG 1 cut(s) 371
Aor13HI TCCGGA 1 cut(s) 552
AoxI GGCC 6 cut(s) 3, 319, 970, 1026, 1035, 1139
ApaLI GTGCAC 1 cut(s) 990
ApeKI GCWGC 4 cut(s) 391, 452, 746, 924
Asp700I GAANNNNTTC 1 cut(s) 94
AspLEI GCGC 1 cut(s) 804
AspS9I GGNCC 1 cut(s) 1139
AsuC2I CCSGG 1 cut(s) 571
AsuHPI GGTGA 4 cut(s) 76, 241, 473, 484
AvaI CYCGRG 1 cut(s) 371
BaeGI GKGCMC 1 cut(s) 994
BaeI ACNNNNGTAYC 4 cut(s) 333, 366, 742, 775
BalI TGGCCA 3 cut(s) 972, 1028, 1037
BanI GGYRCC 2 cut(s) 599, 801
Bbv12I GWGCWC 1 cut(s) 994
BbvI GCAGC 4 cut(s) 403, 439, 733, 911
BccI CCATC 8 cut(s) 52, 626, 662, 746, 749, 1091, 1187, 1217
BceAI ACGGC 1 cut(s) 1000
BciT130I CCWGG 2 cut(s) 974, 998
BciVI GTATCC 1 cut(s) 770
BclI TGATCA 1 cut(s) 1047
BcnI CCSGG 1 cut(s) 571
BcoDI GTCTC 3 cut(s) 166, 438, 1178
BcuI ACTAGT 1 cut(s) 1271
BfaI CTAG 4 cut(s) 323, 614, 1175, 1272
BfmI CTRYAG 1 cut(s) 1278
BfoI RGCGCY 1 cut(s) 805
BfuI GTATCC 1 cut(s) 770
BglI GCCNNNNNGGC 1 cut(s) 1034
BglII AGATCT 1 cut(s) 294
BisI GCNGC 8 cut(s) 309, 392, 453, 747, 805, 820, 823, 925
BlsI GCNGC 8 cut(s) 310, 393, 454, 748, 806, 821, 824, 926
BmcAI AGTACT 2 cut(s) 869, 1276
Bme1390I CCNGG 3 cut(s) 571, 974, 998
BmeT110I CYCGRG 1 cut(s) 371
BmgT120I GGNCC 1 cut(s) 1139
BmiI GGNNCC 4 cut(s) 337, 601, 726, 803
BmrFI CCNGG 3 cut(s) 571, 974, 998
BmrI ACTGGG 2 cut(s) 548, 1221
BmsI GCATC 2 cut(s) 273, 772
BmuI ACTGGG 2 cut(s) 548, 1221
BpmI CTGGAG 1 cut(s) 980
Bpu10I CCTNAGC 1 cut(s) 953
BpuEI CTTGAG 2 cut(s) 380, 1278
BpuMI CCSGG 1 cut(s) 571
BsaHI GRCGYC 2 cut(s) 159, 802
BsaJI CCNNGG 2 cut(s) 774, 997
BsaWI WCCGGW 1 cut(s) 552
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bsc4I CCNNNNNNNGG 2 cut(s) 539, 898
Bse118I RCCGGY 1 cut(s) 859
Bse1I ACTGG 5 cut(s) 82, 543, 829, 1029, 1216
Bse3DI GCAATG 2 cut(s) 303, 741
BseAI TCCGGA 1 cut(s) 552
BseBI CCWGG 2 cut(s) 974, 998
BseDI CCNNGG 2 cut(s) 774, 997
BseGI GGATG 4 cut(s) 222, 636, 1198, 1250
BseLI CCNNNNNNNGG 2 cut(s) 539, 898
BseMI GCAATG 2 cut(s) 303, 741
BseMII CTCAG 3 cut(s) 660, 699, 1179
BseNI ACTGG 5 cut(s) 82, 543, 829, 1029, 1216
BseRI GAGGAG 2 cut(s) 199, 259
BseSI GKGCMC 1 cut(s) 994
BseXI GCAGC 4 cut(s) 403, 439, 733, 911
BsgI GTGCAG 2 cut(s) 910, 998
Bsh1236I CGCG 2 cut(s) 722, 819
BshFI GGCC 6 cut(s) 5, 321, 972, 1028, 1037, 1141
BshNI GGYRCC 2 cut(s) 599, 801
BsiHKAI GWGCWC 1 cut(s) 994
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 4 cut(s) 333, 553, 570, 860
BslFI GGGAC 1 cut(s) 509
BslI CCNNNNNNNGG 2 cut(s) 539, 898
BsmAI GTCTC 3 cut(s) 166, 438, 1178
BsmBI CGTCTC 1 cut(s) 166
BsmFI GGGAC 1 cut(s) 509
BsnI GGCC 6 cut(s) 5, 321, 972, 1028, 1037, 1141
BsoBI CYCGRG 1 cut(s) 371
Bsp1286I GDGCHC 1 cut(s) 994
Bsp13I TCCGGA 1 cut(s) 552
Bsp143I GATC 5 cut(s) 294, 729, 789, 1047, 1055
BspACI CCGC 9 cut(s) 252, 309, 722, 749, 805, 819, 822, 899, 987
BspANI GGCC 6 cut(s) 5, 321, 972, 1028, 1037, 1141
BspCNI CTCAG 3 cut(s) 659, 698, 1180
BspEI TCCGGA 1 cut(s) 552
BspFNI CGCG 2 cut(s) 722, 819
BspHI TCATGA 1 cut(s) 436
BspLI GGNNCC 4 cut(s) 337, 601, 726, 803
BspMAI CTGCAG 1 cut(s) 1282
BspPI GGATC 2 cut(s) 784, 1063
BspT107I GGYRCC 2 cut(s) 599, 801
BsrDI GCAATG 2 cut(s) 303, 741
BsrFI RCCGGY 1 cut(s) 859
BsrI ACTGG 5 cut(s) 82, 543, 829, 1029, 1216
BssAI RCCGGY 1 cut(s) 859
BssECI CCNNGG 2 cut(s) 774, 997
BssMI GATC 5 cut(s) 294, 729, 789, 1047, 1055
BssNI GRCGYC 2 cut(s) 159, 802
Bst2UI CCWGG 2 cut(s) 974, 998
Bst4CI ACNGT 5 cut(s) 260, 500, 667, 850, 905
Bst6I CTCTTC 2 cut(s) 34, 1176
BstACI GRCGYC 2 cut(s) 159, 802
BstC8I GCNNGC 1 cut(s) 409
BstDEI CTNAG 6 cut(s) 646, 685, 864, 953, 1086, 1188
BstF5I GGATG 4 cut(s) 222, 636, 1198, 1250
BstFNI CGCG 2 cut(s) 722, 819
BstH2I RGCGCY 1 cut(s) 805
BstHHI GCGC 1 cut(s) 804
BstKTI GATC 5 cut(s) 297, 732, 792, 1050, 1058
BstMAI GTCTC 3 cut(s) 166, 438, 1178
BstMBI GATC 5 cut(s) 294, 729, 789, 1047, 1055
BstMWI GCNNNNNNNGC 3 cut(s) 743, 905, 1034
BstNI CCWGG 2 cut(s) 974, 998
BstSCI CCNGG 3 cut(s) 569, 972, 996
BstSFI CTRYAG 1 cut(s) 1278
BstSLI GKGCMC 1 cut(s) 994
BstUI CGCG 2 cut(s) 722, 819
BstV1I GCAGC 4 cut(s) 403, 439, 733, 911
BstX2I RGATCY 1 cut(s) 294
BstYI RGATCY 1 cut(s) 294
BsuI GTATCC 1 cut(s) 770
BsuRI GGCC 6 cut(s) 5, 321, 972, 1028, 1037, 1141
BtsCI GGATG 4 cut(s) 222, 636, 1198, 1250
BtsI GCAGTG 2 cut(s) 564, 1020
BtsIMutI CAGTG 7 cut(s) 75, 564, 846, 927, 1020, 1036, 1057
Cac8I GCNNGC 1 cut(s) 409
CciI TCATGA 1 cut(s) 436
CfoI GCGC 1 cut(s) 804
Cfr10I RCCGGY 1 cut(s) 859
Cfr13I GGNCC 1 cut(s) 1139
Csp6I GTAC 7 cut(s) 868, 916, 937, 982, 1209, 1269, 1275
CviAII CATG 5 cut(s) 194, 412, 437, 512, 1010
CviQI GTAC 7 cut(s) 868, 916, 937, 982, 1209, 1269, 1275
DdeI CTNAG 6 cut(s) 646, 685, 864, 953, 1086, 1188
DinI GGCGCC 1 cut(s) 803
DpnI GATC 5 cut(s) 296, 731, 791, 1049, 1057
DpnII GATC 5 cut(s) 294, 729, 789, 1047, 1055
EaeI YGGCCR 3 cut(s) 970, 1026, 1035
Eam1104I CTCTTC 2 cut(s) 34, 1176
EarI CTCTTC 2 cut(s) 34, 1176
Eco88I CYCGRG 1 cut(s) 371
EcoRII CCWGG 2 cut(s) 972, 996
EcoT22I ATGCAT 1 cut(s) 701
EgeI GGCGCC 1 cut(s) 803
EheI GGCGCC 1 cut(s) 803
Esp3I CGTCTC 1 cut(s) 166
FaeI CATG 5 cut(s) 197, 415, 440, 515, 1013
FaqI GGGAC 1 cut(s) 509
FatI CATG 5 cut(s) 193, 411, 436, 511, 1009
FauNDI CATATG 1 cut(s) 594
FbaI TGATCA 1 cut(s) 1047
Fnu4HI GCNGC 8 cut(s) 309, 392, 453, 747, 805, 820, 823, 925
FokI GGATG 4 cut(s) 209, 623, 1205, 1237
Fsp4HI GCNGC 8 cut(s) 309, 392, 453, 747, 805, 820, 823, 925
FspBI CTAG 4 cut(s) 323, 614, 1175, 1272
GlaI GCGC 1 cut(s) 803
GluI GCNGC 8 cut(s) 309, 392, 453, 747, 805, 820, 823, 925
GsuI CTGGAG 1 cut(s) 980
HaeII RGCGCY 1 cut(s) 805
HaeIII GGCC 6 cut(s) 5, 321, 972, 1028, 1037, 1141
HapII CCGG 4 cut(s) 333, 553, 570, 860
HhaI GCGC 1 cut(s) 804
Hin1I GRCGYC 2 cut(s) 159, 802
Hin1II CATG 5 cut(s) 197, 415, 440, 515, 1013
Hin6I GCGC 1 cut(s) 802
HinP1I GCGC 1 cut(s) 802
HindIII AAGCTT 1 cut(s) 405
HinfI GANTC 6 cut(s) 681, 795, 853, 1064, 1091, 1216
HpaII CCGG 4 cut(s) 333, 553, 570, 860
HphI GGTGA 4 cut(s) 76, 241, 473, 484
Hpy166II GTNNAC 3 cut(s) 328, 378, 992
Hpy188I TCNGA 5 cut(s) 340, 688, 778, 794, 1096
Hpy188III TCNNGA 5 cut(s) 397, 437, 553, 880, 1220
Hpy8I GTNNAC 3 cut(s) 328, 378, 992
Hpy99I CGWCG 2 cut(s) 147, 1072
HpyAV CCTTC 3 cut(s) 177, 181, 463
HpyCH4III ACNGT 5 cut(s) 260, 500, 667, 850, 905
HpyCH4IV ACGT 3 cut(s) 145, 159, 810
HpyF10VI GCNNNNNNNGC 3 cut(s) 743, 905, 1034
HpyF3I CTNAG 6 cut(s) 646, 685, 864, 953, 1086, 1188
HpySE526I ACGT 3 cut(s) 145, 159, 810
Hsp92I GRCGYC 2 cut(s) 159, 802
Hsp92II CATG 5 cut(s) 197, 415, 440, 515, 1013
HspAI GCGC 1 cut(s) 802
KasI GGCGCC 1 cut(s) 801
Kpn2I TCCGGA 1 cut(s) 552
Ksp22I TGATCA 1 cut(s) 1047
Kzo9I GATC 5 cut(s) 294, 729, 789, 1047, 1055
LmnI GCTCC 2 cut(s) 341, 724
Lsp1109I GCAGC 4 cut(s) 403, 439, 733, 911
LweI GCATC 2 cut(s) 273, 772
MaeI CTAG 4 cut(s) 323, 614, 1175, 1272
MaeII ACGT 3 cut(s) 145, 159, 810
MaeIII GTNAC 6 cut(s) 461, 850, 1130, 1153, 1246, 1304
MalI GATC 5 cut(s) 296, 731, 791, 1049, 1057
MboI GATC 5 cut(s) 294, 729, 789, 1047, 1055
MboII GAAGA 3 cut(s) 21, 64, 1193
MflI RGATCY 1 cut(s) 294
MhlI GDGCHC 1 cut(s) 994
MlsI TGGCCA 3 cut(s) 972, 1028, 1037
MluCI AATT 1 cut(s) 122
MluNI TGGCCA 3 cut(s) 972, 1028, 1037
Mly113I GGCGCC 1 cut(s) 802
MlyI GAGTC 5 cut(s) 804, 847, 1058, 1085, 1225
Mox20I TGGCCA 3 cut(s) 972, 1028, 1037
Mph1103I ATGCAT 1 cut(s) 701
MroI TCCGGA 1 cut(s) 552
MroXI GAANNNNTTC 1 cut(s) 94
MscI TGGCCA 3 cut(s) 972, 1028, 1037
MslI CAYNNNNRTG 4 cut(s) 416, 597, 1008, 1110
Msp20I TGGCCA 3 cut(s) 972, 1028, 1037
MspA1I CMGCKG 2 cut(s) 899, 1061
MspI CCGG 4 cut(s) 333, 553, 570, 860
MspR9I CCNGG 3 cut(s) 571, 974, 998
MvaI CCWGG 2 cut(s) 974, 998
MvnI CGCG 2 cut(s) 722, 819
MwoI GCNNNNNNNGC 3 cut(s) 743, 905, 1034
NarI GGCGCC 1 cut(s) 802
NciI CCSGG 1 cut(s) 571
NdeI CATATG 1 cut(s) 594
NdeII GATC 5 cut(s) 294, 729, 789, 1047, 1055
NlaIII CATG 5 cut(s) 197, 415, 440, 515, 1013
NlaIV GGNNCC 4 cut(s) 337, 601, 726, 803
NmuCI GTSAC 6 cut(s) 461, 850, 1130, 1153, 1246, 1304
NsiI ATGCAT 1 cut(s) 701
PagI TCATGA 1 cut(s) 436
PdmI GAANNNNTTC 1 cut(s) 94
PfeI GAWTC 1 cut(s) 681
PkrI GCNGC 8 cut(s) 310, 393, 454, 748, 806, 821, 824, 926
PleI GAGTC 5 cut(s) 803, 847, 1058, 1085, 1224
PluTI GGCGCC 1 cut(s) 805
PpsI GAGTC 5 cut(s) 803, 847, 1058, 1085, 1224
Psp1406I AACGTT 1 cut(s) 810
Psp6I CCWGG 2 cut(s) 972, 996
PspGI CCWGG 2 cut(s) 972, 996
PspN4I GGNNCC 4 cut(s) 337, 601, 726, 803
PspPI GGNCC 1 cut(s) 1139
PstI CTGCAG 1 cut(s) 1282
PsuI RGATCY 1 cut(s) 294
PvuII CAGCTG 1 cut(s) 1061
RsaI GTAC 7 cut(s) 869, 917, 938, 983, 1210, 1270, 1276
RsaNI GTAC 7 cut(s) 868, 916, 937, 982, 1209, 1269, 1275
RseI CAYNNNNRTG 4 cut(s) 416, 597, 1008, 1110
SatI GCNGC 8 cut(s) 309, 392, 453, 747, 805, 820, 823, 925
Sau3AI GATC 5 cut(s) 294, 729, 789, 1047, 1055
Sau96I GGNCC 1 cut(s) 1139
ScaI AGTACT 2 cut(s) 869, 1276
SchI GAGTC 5 cut(s) 804, 847, 1058, 1085, 1225
ScrFI CCNGG 3 cut(s) 571, 974, 998
SduI GDGCHC 1 cut(s) 994
SfaNI GCATC 2 cut(s) 273, 772
SfcI CTRYAG 1 cut(s) 1278
SfiI GGCCNNNNNGGCC 1 cut(s) 1034
SfoI GGCGCC 1 cut(s) 803
SmiMI CAYNNNNRTG 4 cut(s) 416, 597, 1008, 1110
SmlI CTYRAG 2 cut(s) 395, 1293
SmoI CTYRAG 2 cut(s) 395, 1293
SpeI ACTAGT 1 cut(s) 1271
Sse9I AATT 1 cut(s) 122
SsiI CCGC 9 cut(s) 252, 309, 722, 749, 805, 819, 822, 899, 987
SspDI GGCGCC 1 cut(s) 801
SspMI CTAG 4 cut(s) 323, 614, 1175, 1272
StyD4I CCNGG 3 cut(s) 569, 972, 996
TaaI ACNGT 5 cut(s) 260, 500, 667, 850, 905
TaiI ACGT 3 cut(s) 148, 162, 813
TaqI TCGA 3 cut(s) 142, 657, 1067
TasI AATT 1 cut(s) 122
TatI WGTACW 3 cut(s) 867, 1208, 1274
TauI GCSGC 4 cut(s) 311, 807, 822, 825
TfiI GAWTC 1 cut(s) 681
TscAI CASTG 7 cut(s) 82, 571, 853, 934, 1020, 1036, 1057
TseFI GTSAC 6 cut(s) 461, 850, 1130, 1153, 1246, 1304
TseI GCWGC 4 cut(s) 391, 452, 746, 924
Tsp45I GTSAC 6 cut(s) 461, 850, 1130, 1153, 1246, 1304
TspDTI ATGAA 2 cut(s) 211, 602
TspGWI ACGGA 1 cut(s) 262
TspRI CASTG 7 cut(s) 82, 571, 853, 934, 1020, 1036, 1057
VneI GTGCAC 1 cut(s) 990
XcmI CCANNNNNNNNNTGG 2 cut(s) 409, 535
XmnI GAANNNNTTC 1 cut(s) 94
XspI CTAG 4 cut(s) 323, 614, 1175, 1272
ZraI GACGTC 1 cut(s) 160
ZrmI AGTACT 2 cut(s) 869, 1276
Zsp2I ATGCAT 1 cut(s) 701
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.