Rh2DG426600

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001993
Physical Location & Seq
Forward (+)
61799816 .. 61803485
3670 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG426600.1

Sequence Viewer

Length: 1311 bp
ATGGCCTCACAAGACTACTCCCCCAACTCTCCCCCAACTCCATCCTTTGCAACAAATAAAAACAAGTTTTTCTCCGGCTTCACCAGAAGCACATCAACCAAAGAACCCTCCTTCTCACAATTTCCTTATACACCGCCTCGCCATAACACCAATGACGTCTCTCCTTCCTTCTCCGAAACCGAAAGCCTCCACGCCTGCCCAACCTCCTCCCCTCAGTTCATCCCTCTCAAACACCCCGACCTCCTCACCGCCTACCGTTGCATCTCCTCCGTCCTGAAAAAGGACGGCCGGATCTTGTCCATCACCGCCTCCAGTGGCCTAGTTTACTCCGGCTCAGATACCAATGTCATTAGGGTTTGGAAGCTACCCGAGTTCACAGAATGTGGGCAGCTCAAGACCAAAGCTTGCATGGTGGTGGCACTAGAGGTGTCTCATGATAGGGTTTATGCTGCTTATGGTGATGGGAAGGTGAGAATATGGCAGAGAACATGGGATGGAGGGTTGAAGCATGAGAGGTTGGCTACGATTCCGAAGATCGGAAGTTATGTCCGGAGTTTCTACATTGCCGGGAAAGATAAGACGGTTAAGCATATGGGTGCCATAACATCACTAGCCATCAATGTAGCAGAGGACATCCTCTACTCAGCTTCCATTGACAAAACAGTGAAAGTATGGCGAATTTCAGACCTCAAATGCATCGAGACAATCCAAGCCCACGCGGAGCCAATCAACGCCATCATTGCCGCGGACGATGGAATCCTCTACACGGCCTCCGACGATGCAACTGTTCGAGTCTGGCGCCGCAACTTTTGTCGTGGCGACCAGCTTCCACATTCACTCACAGTGACTCTACCGGCCAAGTACTCCCCCGTCAAGACCCTAACCCTAACAGCGGATGGTGCAATCTTGTACGGGGGTTGCACTGACGGGTACATACACTTTTGGCTCAGGGGTTGGTTCTCAGGCCAGGTGCAGTACGGCGGGGCACTCCAGGGCCACACCCATGCAGTTATGTGCTTGGCCAGCGTGGCGAACTACGTGGTCAGTGGGTCAGCTGACTCAACGAGTAGGATTTGGTCTAAGGACCCTGATGGTCTGCATACTTGTCTAGCAGTCCTTGTGGGTCACAGAGGGCCAATCAGGTGTGTGACAGCATTTCTGGGAAGGCTAGGAGAAGAGACTGAGGAGGGTTGCACAATCTGTACAGGGAGTCTTGATGGTGTTCTCAAAGTGTGGCGTGTGACATCCAAAAGTAATAGTGGTAATAGTGGGTGTTTAGCACAGGGTGGGAGTGAGTATTTCGAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

436

Amino Acids

47.53

Weight (kDa)

8.47

Isoelectric Point (pI)

37.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_EML PF23409 111 - 305 1.5e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR36-Utp21_2nd PF25168 184 - 265 3.2e-06 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 194 - 359 8.2e-12 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 195 - 414 4.2e-19 THOC3 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 195 - 268 8.2e-08 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 196 - 268 2.1e-14 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 196 - 267 3.5e-12 WDR3 first beta-propeller domain
WDR55 PF24796 196 - 421 2.1e-10 WDR55
WD40_Prp19 PF24814 199 - 315 1.6e-11 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 203 - 357 1.3e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 282 - 417 3.1e-10 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 301 - 416 3.6e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 328 - 416 9.6e-10 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 159
AccB1I GGYRCC 2 cut(s) 596, 798
AccII CGCG 2 cut(s) 719, 746
AccIII TCCGGA 1 cut(s) 549
AciI CCGC 9 cut(s) 134, 249, 306, 719, 744, 746, 802, 893, 981
AclWI GGATC 1 cut(s) 299
AcoI YGGCCR 3 cut(s) 286, 855, 1020
AcsI RAATTY 1 cut(s) 678
AcyI GRCGYC 2 cut(s) 156, 799
AdeI CACNNNGTG 1 cut(s) 1286
AfaI GTAC 5 cut(s) 863, 911, 932, 977, 1204
AfiI CCNNNNNNNGG 4 cut(s) 280, 536, 766, 892
AgsI TTSAA 1 cut(s) 505
AjnI CCWGG 2 cut(s) 966, 990
AluBI AGCT 7 cut(s) 364, 391, 404, 647, 826, 1055, 1306
AluI AGCT 7 cut(s) 364, 391, 404, 647, 826, 1055, 1306
Alw26I GTCTC 4 cut(s) 163, 435, 695, 1172
AlwI GGATC 1 cut(s) 299
Ama87I CYCGRG 1 cut(s) 368
Aor13HI TCCGGA 1 cut(s) 549
AoxI GGCC 9 cut(s) 3, 286, 316, 768, 855, 964, 994, 1020, 1133
ApeKI GCWGC 2 cut(s) 388, 449
ApoI RAATTY 1 cut(s) 678
AspLEI GCGC 1 cut(s) 801
AspS9I GGNCC 3 cut(s) 994, 1084, 1133
AsuC2I CCSGG 1 cut(s) 568
AsuHPI GGTGA 5 cut(s) 73, 238, 295, 470, 481
AvaI CYCGRG 1 cut(s) 368
AvaII GGWCC 1 cut(s) 1084
BaeGI GKGCMC 1 cut(s) 988
BaeI ACNNNNGTAYC 2 cut(s) 330, 363
BalI TGGCCA 1 cut(s) 1022
BanI GGYRCC 2 cut(s) 596, 798
BbvI GCAGC 2 cut(s) 400, 436
BceAI ACGGC 3 cut(s) 301, 783, 994
BciT130I CCWGG 2 cut(s) 968, 992
BcnI CCSGG 1 cut(s) 568
BcoDI GTCTC 4 cut(s) 163, 435, 695, 1172
BfaI CTAG 5 cut(s) 320, 422, 611, 1109, 1169
BfmI CTRYAG 1 cut(s) 1307
BfoI RGCGCY 1 cut(s) 802
BglI GCCNNNNNGGC 1 cut(s) 1028
BisI GCNGC 4 cut(s) 389, 450, 744, 802
BlsI GCNGC 4 cut(s) 390, 451, 745, 803
BmcAI AGTACT 1 cut(s) 863
Bme1390I CCNGG 3 cut(s) 568, 968, 992
Bme18I GGWCC 1 cut(s) 1084
BmeT110I CYCGRG 1 cut(s) 368
BmgT120I GGNCC 3 cut(s) 994, 1084, 1133
BmiI GGNNCC 4 cut(s) 598, 723, 800, 1086
BmrFI CCNGG 3 cut(s) 568, 968, 992
BmsI GCATC 3 cut(s) 270, 705, 769
BpmI CTGGAG 2 cut(s) 295, 974
Bpu10I CCTNAGC 1 cut(s) 947
BpuEI CTTGAG 1 cut(s) 377
BpuMI CCSGG 1 cut(s) 568
BsaAI YACGTR 1 cut(s) 1039
BsaHI GRCGYC 2 cut(s) 156, 799
BsaJI CCNNGG 2 cut(s) 744, 991
BsaWI WCCGGW 1 cut(s) 549
BsaXI ACNNNNNCTCC 2 cut(s) 755, 785
Bsc4I CCNNNNNNNGG 4 cut(s) 280, 536, 766, 892
Bse118I RCCGGY 1 cut(s) 853
Bse1I ACTGG 1 cut(s) 312
Bse3DI GCAATG 2 cut(s) 561, 738
BseAI TCCGGA 1 cut(s) 549
BseBI CCWGG 2 cut(s) 968, 992
BseDI CCNNGG 2 cut(s) 744, 991
BseGI GGATG 6 cut(s) 41, 219, 499, 633, 901, 1244
BseLI CCNNNNNNNGG 4 cut(s) 280, 536, 766, 892
BseMI GCAATG 2 cut(s) 561, 738
BseMII CTCAG 6 cut(s) 227, 348, 657, 961, 975, 1173
BseNI ACTGG 1 cut(s) 312
BseRI GAGGAG 4 cut(s) 196, 233, 256, 1199
BseSI GKGCMC 1 cut(s) 988
BseX3I CGGCCG 1 cut(s) 286
BseXI GCAGC 2 cut(s) 400, 436
BsgI GTGCAG 1 cut(s) 992
Bsh1236I CGCG 2 cut(s) 719, 746
Bsh1285I CGRYCG 1 cut(s) 289
BshFI GGCC 9 cut(s) 5, 288, 318, 770, 857, 966, 996, 1022, 1135
BshNI GGYRCC 2 cut(s) 596, 798
BsiEI CGRYCG 1 cut(s) 289
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 6 cut(s) 75, 289, 330, 550, 567, 854
BslI CCNNNNNNNGG 4 cut(s) 280, 536, 766, 892
BsmAI GTCTC 4 cut(s) 163, 435, 695, 1172
BsmBI CGTCTC 1 cut(s) 163
BsnI GGCC 9 cut(s) 5, 288, 318, 770, 857, 966, 996, 1022, 1135
BsoBI CYCGRG 1 cut(s) 368
Bsp1286I GDGCHC 1 cut(s) 988
Bsp13I TCCGGA 1 cut(s) 549
Bsp1407I TGTACA 1 cut(s) 1202
Bsp143I GATC 2 cut(s) 291, 534
BspACI CCGC 9 cut(s) 134, 249, 306, 719, 744, 746, 802, 893, 981
BspANI GGCC 9 cut(s) 5, 288, 318, 770, 857, 966, 996, 1022, 1135
BspCNI CTCAG 6 cut(s) 226, 347, 656, 960, 974, 1174
BspEI TCCGGA 1 cut(s) 549
BspFNI CGCG 2 cut(s) 719, 746
BspHI TCATGA 1 cut(s) 433
BspLI GGNNCC 4 cut(s) 598, 723, 800, 1086
BspPI GGATC 1 cut(s) 299
BspT107I GGYRCC 2 cut(s) 596, 798
BsrDI GCAATG 2 cut(s) 561, 738
BsrFI RCCGGY 1 cut(s) 853
BsrGI TGTACA 1 cut(s) 1202
BsrI ACTGG 1 cut(s) 312
BssAI RCCGGY 1 cut(s) 853
BssECI CCNNGG 2 cut(s) 744, 991
BssMI GATC 2 cut(s) 291, 534
BssNI GRCGYC 2 cut(s) 156, 799
Bst2UI CCWGG 2 cut(s) 968, 992
Bst4CI ACNGT 5 cut(s) 257, 583, 664, 787, 844
Bst6I CTCTTC 1 cut(s) 1170
BstACI GRCGYC 2 cut(s) 156, 799
BstAUI TGTACA 1 cut(s) 1202
BstBAI YACGTR 1 cut(s) 1039
BstC8I GCNNGC 3 cut(s) 196, 406, 1024
BstDEI CTNAG 7 cut(s) 213, 334, 643, 947, 961, 1080, 1182
BstDSI CCRYGG 1 cut(s) 744
BstENI CCTNNNNNAGG 1 cut(s) 278
BstF5I GGATG 6 cut(s) 41, 219, 499, 633, 901, 1244
BstFNI CGCG 2 cut(s) 719, 746
BstH2I RGCGCY 1 cut(s) 802
BstHHI GCGC 1 cut(s) 801
BstKTI GATC 2 cut(s) 294, 537
BstMAI GTCTC 4 cut(s) 163, 435, 695, 1172
BstMBI GATC 2 cut(s) 291, 534
BstMCI CGRYCG 1 cut(s) 289
BstMWI GCNNNNNNNGC 4 cut(s) 740, 899, 1023, 1028
BstNI CCWGG 2 cut(s) 968, 992
BstSCI CCNGG 3 cut(s) 566, 966, 990
BstSFI CTRYAG 1 cut(s) 1307
BstSLI GKGCMC 1 cut(s) 988
BstUI CGCG 2 cut(s) 719, 746
BstV1I GCAGC 2 cut(s) 400, 436
BstX2I RGATCY 1 cut(s) 291
BstYI RGATCY 1 cut(s) 291
BstZI CGGCCG 1 cut(s) 286
BsuRI GGCC 9 cut(s) 5, 288, 318, 770, 857, 966, 996, 1022, 1135
BtgI CCRYGG 1 cut(s) 744
BtsCI GGATG 6 cut(s) 41, 219, 499, 633, 901, 1244
BtsIMutI CAGTG 5 cut(s) 319, 669, 849, 921, 1051
Cac8I GCNNGC 3 cut(s) 196, 406, 1024
CciI TCATGA 1 cut(s) 433
CfoI GCGC 1 cut(s) 801
Cfr10I RCCGGY 1 cut(s) 853
Cfr13I GGNCC 3 cut(s) 994, 1084, 1133
Cfr42I CCGCGG 1 cut(s) 747
Csp6I GTAC 5 cut(s) 862, 910, 931, 976, 1203
CviAII CATG 5 cut(s) 409, 434, 489, 509, 1004
CviQI GTAC 5 cut(s) 862, 910, 931, 976, 1203
DdeI CTNAG 7 cut(s) 213, 334, 643, 947, 961, 1080, 1182
DinI GGCGCC 1 cut(s) 800
DpnI GATC 2 cut(s) 293, 536
DpnII GATC 2 cut(s) 291, 534
DraIII CACNNNGTG 1 cut(s) 1286
EaeI YGGCCR 3 cut(s) 286, 855, 1020
EagI CGGCCG 1 cut(s) 286
Eam1104I CTCTTC 1 cut(s) 1170
EarI CTCTTC 1 cut(s) 1170
EclXI CGGCCG 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 1084
Eco52I CGGCCG 1 cut(s) 286
Eco88I CYCGRG 1 cut(s) 368
EcoNI CCTNNNNNAGG 1 cut(s) 278
EcoO109I RGGNCCY 1 cut(s) 1084
EcoRII CCWGG 2 cut(s) 966, 990
EcoT22I ATGCAT 1 cut(s) 698
EgeI GGCGCC 1 cut(s) 800
EheI GGCGCC 1 cut(s) 800
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 5 cut(s) 412, 437, 492, 512, 1007
FatI CATG 5 cut(s) 408, 433, 488, 508, 1003
FauI CCCGC 1 cut(s) 974
FauNDI CATATG 1 cut(s) 591
Fnu4HI GCNGC 4 cut(s) 389, 450, 744, 802
FokI GGATG 6 cut(s) 28, 206, 506, 620, 908, 1231
Fsp4HI GCNGC 4 cut(s) 389, 450, 744, 802
FspBI CTAG 5 cut(s) 320, 422, 611, 1109, 1169
GlaI GCGC 1 cut(s) 800
GluI GCNGC 4 cut(s) 389, 450, 744, 802
GsuI CTGGAG 2 cut(s) 295, 974
HaeII RGCGCY 1 cut(s) 802
HaeIII GGCC 9 cut(s) 5, 288, 318, 770, 857, 966, 996, 1022, 1135
HapII CCGG 6 cut(s) 75, 289, 330, 550, 567, 854
HhaI GCGC 1 cut(s) 801
Hin1I GRCGYC 2 cut(s) 156, 799
Hin1II CATG 5 cut(s) 412, 437, 492, 512, 1007
Hin6I GCGC 1 cut(s) 799
HinP1I GCGC 1 cut(s) 799
HindIII AAGCTT 1 cut(s) 402
HinfI GANTC 6 cut(s) 526, 756, 792, 847, 1058, 1210
HpaII CCGG 6 cut(s) 75, 289, 330, 550, 567, 854
HphI GGTGA 5 cut(s) 73, 238, 295, 470, 481
Hpy166II GTNNAC 2 cut(s) 325, 375
Hpy188I TCNGA 6 cut(s) 175, 337, 531, 539, 685, 775
Hpy188III TCNNGA 7 cut(s) 274, 394, 434, 550, 700, 874, 1214
Hpy8I GTNNAC 2 cut(s) 325, 375
Hpy99I CGWCG 1 cut(s) 779
HpyAV CCTTC 5 cut(s) 121, 174, 178, 460, 1158
HpyCH4III ACNGT 5 cut(s) 257, 583, 664, 787, 844
HpyCH4IV ACGT 2 cut(s) 156, 1038
HpyF10VI GCNNNNNNNGC 4 cut(s) 740, 899, 1023, 1028
HpyF3I CTNAG 7 cut(s) 213, 334, 643, 947, 961, 1080, 1182
HpySE526I ACGT 2 cut(s) 156, 1038
Hsp92I GRCGYC 2 cut(s) 156, 799
Hsp92II CATG 5 cut(s) 412, 437, 492, 512, 1007
HspAI GCGC 1 cut(s) 799
KasI GGCGCC 1 cut(s) 798
Kpn2I TCCGGA 1 cut(s) 549
KspI CCGCGG 1 cut(s) 747
Kzo9I GATC 2 cut(s) 291, 534
LmnI GCTCC 1 cut(s) 721
Lsp1109I GCAGC 2 cut(s) 400, 436
LweI GCATC 3 cut(s) 270, 705, 769
MaeI CTAG 5 cut(s) 320, 422, 611, 1109, 1169
MaeII ACGT 2 cut(s) 156, 1038
MaeIII GTNAC 4 cut(s) 844, 1124, 1147, 1240
MalI GATC 2 cut(s) 293, 536
MboI GATC 2 cut(s) 291, 534
MboII GAAGA 2 cut(s) 544, 1187
MflI RGATCY 1 cut(s) 291
MhlI GDGCHC 1 cut(s) 988
MlsI TGGCCA 1 cut(s) 1022
MluCI AATT 2 cut(s) 119, 678
MluNI TGGCCA 1 cut(s) 1022
Mly113I GGCGCC 1 cut(s) 799
MlyI GAGTC 4 cut(s) 801, 841, 1052, 1219
MmeI TCCRAC 1 cut(s) 798
Mox20I TGGCCA 1 cut(s) 1022
Mph1103I ATGCAT 1 cut(s) 698
MroI TCCGGA 1 cut(s) 549
MscI TGGCCA 1 cut(s) 1022
MseI TTAA 1 cut(s) 585
MslI CAYNNNNRTG 3 cut(s) 413, 594, 1002
Msp20I TGGCCA 1 cut(s) 1022
MspA1I CMGCKG 3 cut(s) 746, 893, 1055
MspI CCGG 6 cut(s) 75, 289, 330, 550, 567, 854
MspR9I CCNGG 3 cut(s) 568, 968, 992
MvaI CCWGG 2 cut(s) 968, 992
MvnI CGCG 2 cut(s) 719, 746
MwoI GCNNNNNNNGC 4 cut(s) 740, 899, 1023, 1028
NarI GGCGCC 1 cut(s) 799
NciI CCSGG 1 cut(s) 568
NdeI CATATG 1 cut(s) 591
NdeII GATC 2 cut(s) 291, 534
NlaIII CATG 5 cut(s) 412, 437, 492, 512, 1007
NlaIV GGNNCC 4 cut(s) 598, 723, 800, 1086
NmuCI GTSAC 4 cut(s) 844, 1124, 1147, 1240
NsiI ATGCAT 1 cut(s) 698
PagI TCATGA 1 cut(s) 433
PfeI GAWTC 2 cut(s) 526, 756
PkrI GCNGC 4 cut(s) 390, 451, 745, 803
PleI GAGTC 4 cut(s) 800, 841, 1052, 1218
PluTI GGCGCC 1 cut(s) 802
PpsI GAGTC 4 cut(s) 800, 841, 1052, 1218
Ppu21I YACGTR 1 cut(s) 1039
PpuMI RGGWCCY 1 cut(s) 1084
Psp5II RGGWCCY 1 cut(s) 1084
Psp6I CCWGG 2 cut(s) 966, 990
PspGI CCWGG 2 cut(s) 966, 990
PspN4I GGNNCC 4 cut(s) 598, 723, 800, 1086
PspPI GGNCC 3 cut(s) 994, 1084, 1133
PspPPI RGGWCCY 1 cut(s) 1084
PsuI RGATCY 1 cut(s) 291
PvuII CAGCTG 1 cut(s) 1055
RsaI GTAC 5 cut(s) 863, 911, 932, 977, 1204
RsaNI GTAC 5 cut(s) 862, 910, 931, 976, 1203
RseI CAYNNNNRTG 3 cut(s) 413, 594, 1002
SacII CCGCGG 1 cut(s) 747
SaqAI TTAA 1 cut(s) 585
SatI GCNGC 4 cut(s) 389, 450, 744, 802
Sau3AI GATC 2 cut(s) 291, 534
Sau96I GGNCC 3 cut(s) 994, 1084, 1133
ScaI AGTACT 1 cut(s) 863
SchI GAGTC 4 cut(s) 801, 841, 1052, 1219
ScrFI CCNGG 3 cut(s) 568, 968, 992
SduI GDGCHC 1 cut(s) 988
SfaNI GCATC 3 cut(s) 270, 705, 769
SfcI CTRYAG 1 cut(s) 1307
SfoI GGCGCC 1 cut(s) 800
Sfr303I CCGCGG 1 cut(s) 747
SgrBI CCGCGG 1 cut(s) 747
SinI GGWCC 1 cut(s) 1084
SmiMI CAYNNNNRTG 3 cut(s) 413, 594, 1002
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
Sse9I AATT 2 cut(s) 119, 678
SsiI CCGC 9 cut(s) 134, 249, 306, 719, 744, 746, 802, 893, 981
SspDI GGCGCC 1 cut(s) 798
SspMI CTAG 5 cut(s) 320, 422, 611, 1109, 1169
StyD4I CCNGG 3 cut(s) 566, 966, 990
TaaI ACNGT 5 cut(s) 257, 583, 664, 787, 844
TaiI ACGT 2 cut(s) 159, 1041
TaqI TCGA 3 cut(s) 699, 790, 1302
TasI AATT 2 cut(s) 119, 678
TatI WGTACW 2 cut(s) 861, 1202
TauI GCSGC 2 cut(s) 746, 804
TfiI GAWTC 2 cut(s) 526, 756
Tru1I TTAA 1 cut(s) 585
Tru9I TTAA 1 cut(s) 585
TscAI CASTG 5 cut(s) 319, 669, 849, 928, 1051
TseFI GTSAC 4 cut(s) 844, 1124, 1147, 1240
TseI GCWGC 2 cut(s) 388, 449
Tsp45I GTSAC 4 cut(s) 844, 1124, 1147, 1240
TspDTI ATGAA 1 cut(s) 208
TspGWI ACGGA 1 cut(s) 259
TspRI CASTG 5 cut(s) 319, 669, 849, 928, 1051
VpaK11BI GGWCC 1 cut(s) 1084
XagI CCTNNNNNAGG 1 cut(s) 278
XapI RAATTY 1 cut(s) 678
XcmI CCANNNNNNNNNTGG 1 cut(s) 406
XspI CTAG 5 cut(s) 320, 422, 611, 1109, 1169
ZraI GACGTC 1 cut(s) 157
ZrmI AGTACT 1 cut(s) 863
Zsp2I ATGCAT 1 cut(s) 698
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.