Rmu_co8444677.1_g000001

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8444677.1
OG0001993
Physical Location & Seq
Forward (+)
1 .. 1117
1117 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8444677.1_g000001.1.cds

Sequence Viewer

Length: 729 bp
gttaagcatatgggtgccataacatcactagccatcaatgtagcagaggacatcctctactcagcttccattgacaaaacagtgaaagtatggcgaatttcagacctcaaatgcatcgagacaatccaagcccacgccgagccaatcaatgccatcattgccgcggacgatggaatcctctacacggcctccgacgatgcaactgttcgagtctggcgccgcaacttttgtcgtggcgaccagcttccacattcactcaccgtgactctaccggccaagtactcccccgtcaagaccctaaccctaacagcggatggtgcaatcttgtacgggggttgcactgacgggtacatacacttttggcttaggggttggttctcaggccaggtgcagtacggcggggcactccagggccacacccatgcagttatgtgcttggccagcgtggcgaactacgtggtcagtgggtcagctgactcaacgagtaggatttggtctaaggaccctgatggtctgcatacttgtctagcagtccttgtgggtcacagagggccaatcaggtgtgtgacagcatttctgggaaggctgggagaagagactgaggaggggtgcacaatctgtacagggagtcttgatggtgttctcaaagtgtggcgtgtgacatccaaaagtaatagtggtaatagtgggtgtttagcacagggtgggagtgagtatttcgagctgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

242

Amino Acids

25.98

Weight (kDa)

6.24

Isoelectric Point (pI)

28.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 14, 216
AccII CGCG 1 cut(s) 164
AciI CCGC 5 cut(s) 162, 164, 220, 311, 399
AcoI YGGCCR 2 cut(s) 273, 438
AcsI RAATTY 1 cut(s) 96
AcyI GRCGYC 1 cut(s) 217
AdeI CACNNNGTG 1 cut(s) 704
AfaI GTAC 5 cut(s) 281, 329, 350, 395, 622
AfiI CCNNNNNNNGG 2 cut(s) 184, 310
AjnI CCWGG 2 cut(s) 384, 408
AluBI AGCT 4 cut(s) 65, 244, 473, 724
AluI AGCT 4 cut(s) 65, 244, 473, 724
Alw21I GWGCWC 1 cut(s) 614
Alw26I GTCTC 2 cut(s) 113, 590
Alw44I GTGCAC 1 cut(s) 610
AoxI GGCC 6 cut(s) 186, 273, 382, 412, 438, 551
ApaLI GTGCAC 1 cut(s) 610
ApoI RAATTY 1 cut(s) 96
AspLEI GCGC 1 cut(s) 219
AspS9I GGNCC 3 cut(s) 412, 502, 551
AsuHPI GGTGA 1 cut(s) 250
AvaII GGWCC 1 cut(s) 502
BaeGI GKGCMC 2 cut(s) 406, 614
BalI TGGCCA 1 cut(s) 440
BanI GGYRCC 2 cut(s) 14, 216
Bbv12I GWGCWC 1 cut(s) 614
BccI CCATC 6 cut(s) 41, 161, 164, 308, 503, 629
BceAI ACGGC 2 cut(s) 201, 412
BciT130I CCWGG 2 cut(s) 386, 410
BcoDI GTCTC 2 cut(s) 113, 590
BfaI CTAG 2 cut(s) 29, 527
BfmI CTRYAG 1 cut(s) 725
BfoI RGCGCY 1 cut(s) 220
BglI GCCNNNNNGGC 1 cut(s) 446
BisI GCNGC 2 cut(s) 162, 220
BlsI GCNGC 2 cut(s) 163, 221
BmcAI AGTACT 1 cut(s) 281
Bme1390I CCNGG 2 cut(s) 386, 410
Bme18I GGWCC 1 cut(s) 502
BmgT120I GGNCC 3 cut(s) 412, 502, 551
BmiI GGNNCC 3 cut(s) 16, 218, 504
BmrFI CCNGG 2 cut(s) 386, 410
BmsI GCATC 2 cut(s) 123, 187
BpmI CTGGAG 1 cut(s) 392
Bpu10I CCTNAGC 1 cut(s) 365
BsaAI YACGTR 1 cut(s) 457
BsaHI GRCGYC 1 cut(s) 217
BsaJI CCNNGG 2 cut(s) 162, 409
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bsc4I CCNNNNNNNGG 2 cut(s) 184, 310
Bse118I RCCGGY 1 cut(s) 271
Bse3DI GCAATG 1 cut(s) 156
BseBI CCWGG 2 cut(s) 386, 410
BseDI CCNNGG 2 cut(s) 162, 409
BseGI GGATG 3 cut(s) 51, 319, 662
BseLI CCNNNNNNNGG 2 cut(s) 184, 310
BseMI GCAATG 1 cut(s) 156
BseMII CTCAG 3 cut(s) 75, 393, 591
BseRI GAGGAG 1 cut(s) 617
BseSI GKGCMC 2 cut(s) 406, 614
BseYI CCCAGC 1 cut(s) 586
BsgI GTGCAG 1 cut(s) 410
Bsh1236I CGCG 1 cut(s) 164
BshFI GGCC 6 cut(s) 188, 275, 384, 414, 440, 553
BshNI GGYRCC 2 cut(s) 14, 216
BsiHKAI GWGCWC 1 cut(s) 614
BsiSI CCGG 1 cut(s) 272
BslI CCNNNNNNNGG 2 cut(s) 184, 310
BsmAI GTCTC 2 cut(s) 113, 590
BsnI GGCC 6 cut(s) 188, 275, 384, 414, 440, 553
Bsp1286I GDGCHC 2 cut(s) 406, 614
Bsp1407I TGTACA 1 cut(s) 620
BspACI CCGC 5 cut(s) 162, 164, 220, 311, 399
BspANI GGCC 6 cut(s) 188, 275, 384, 414, 440, 553
BspCNI CTCAG 3 cut(s) 74, 392, 592
BspFNI CGCG 1 cut(s) 164
BspLI GGNNCC 3 cut(s) 16, 218, 504
BspT107I GGYRCC 2 cut(s) 14, 216
BsrDI GCAATG 1 cut(s) 156
BsrFI RCCGGY 1 cut(s) 271
BsrGI TGTACA 1 cut(s) 620
BssAI RCCGGY 1 cut(s) 271
BssECI CCNNGG 2 cut(s) 162, 409
BssNI GRCGYC 1 cut(s) 217
Bst2UI CCWGG 2 cut(s) 386, 410
Bst4CI ACNGT 3 cut(s) 82, 205, 262
Bst6I CTCTTC 1 cut(s) 588
BstACI GRCGYC 1 cut(s) 217
BstAUI TGTACA 1 cut(s) 620
BstBAI YACGTR 1 cut(s) 457
BstC8I GCNNGC 1 cut(s) 442
BstDEI CTNAG 5 cut(s) 61, 365, 379, 498, 600
BstDSI CCRYGG 1 cut(s) 162
BstF5I GGATG 3 cut(s) 51, 319, 662
BstFNI CGCG 1 cut(s) 164
BstH2I RGCGCY 1 cut(s) 220
BstHHI GCGC 1 cut(s) 219
BstMAI GTCTC 2 cut(s) 113, 590
BstMWI GCNNNNNNNGC 4 cut(s) 158, 317, 441, 446
BstNI CCWGG 2 cut(s) 386, 410
BstSCI CCNGG 2 cut(s) 384, 408
BstSFI CTRYAG 1 cut(s) 725
BstSLI GKGCMC 2 cut(s) 406, 614
BstUI CGCG 1 cut(s) 164
BsuRI GGCC 6 cut(s) 188, 275, 384, 414, 440, 553
BtgI CCRYGG 1 cut(s) 162
BtsCI GGATG 3 cut(s) 51, 319, 662
BtsIMutI CAGTG 3 cut(s) 87, 339, 469
Cac8I GCNNGC 1 cut(s) 442
CfoI GCGC 1 cut(s) 219
Cfr10I RCCGGY 1 cut(s) 271
Cfr13I GGNCC 3 cut(s) 412, 502, 551
Cfr42I CCGCGG 1 cut(s) 165
Csp6I GTAC 5 cut(s) 280, 328, 349, 394, 621
CviAII CATG 1 cut(s) 422
CviQI GTAC 5 cut(s) 280, 328, 349, 394, 621
DdeI CTNAG 5 cut(s) 61, 365, 379, 498, 600
DinI GGCGCC 1 cut(s) 218
DraIII CACNNNGTG 1 cut(s) 704
EaeI YGGCCR 2 cut(s) 273, 438
Eam1104I CTCTTC 1 cut(s) 588
EarI CTCTTC 1 cut(s) 588
Eco47I GGWCC 1 cut(s) 502
EcoO109I RGGNCCY 1 cut(s) 502
EcoRII CCWGG 2 cut(s) 384, 408
EcoT22I ATGCAT 1 cut(s) 116
EgeI GGCGCC 1 cut(s) 218
EheI GGCGCC 1 cut(s) 218
FaeI CATG 1 cut(s) 425
FaiI YATR 8 cut(s) 9, 11, 20, 91, 353, 423, 431, 519
FatI CATG 1 cut(s) 421
FauI CCCGC 1 cut(s) 392
FauNDI CATATG 1 cut(s) 9
Fnu4HI GCNGC 2 cut(s) 162, 220
FokI GGATG 3 cut(s) 38, 326, 649
Fsp4HI GCNGC 2 cut(s) 162, 220
FspBI CTAG 2 cut(s) 29, 527
GlaI GCGC 1 cut(s) 218
GluI GCNGC 2 cut(s) 162, 220
GsaI CCCAGC 1 cut(s) 590
GsuI CTGGAG 1 cut(s) 392
HaeII RGCGCY 1 cut(s) 220
HaeIII GGCC 6 cut(s) 188, 275, 384, 414, 440, 553
HapII CCGG 1 cut(s) 272
HhaI GCGC 1 cut(s) 219
Hin1I GRCGYC 1 cut(s) 217
Hin1II CATG 1 cut(s) 425
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HinfI GANTC 5 cut(s) 174, 210, 265, 476, 628
HpaII CCGG 1 cut(s) 272
HphI GGTGA 1 cut(s) 250
Hpy166II GTNNAC 1 cut(s) 612
Hpy188I TCNGA 2 cut(s) 103, 193
Hpy188III TCNNGA 3 cut(s) 118, 292, 632
Hpy8I GTNNAC 1 cut(s) 612
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 1 cut(s) 576
HpyCH4III ACNGT 3 cut(s) 82, 205, 262
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 8 cut(s) 114, 200, 320, 339, 391, 425, 517, 612
HpyF10VI GCNNNNNNNGC 4 cut(s) 158, 317, 441, 446
HpyF3I CTNAG 5 cut(s) 61, 365, 379, 498, 600
HpySE526I ACGT 1 cut(s) 456
Hsp92I GRCGYC 1 cut(s) 217
Hsp92II CATG 1 cut(s) 425
HspAI GCGC 1 cut(s) 217
KasI GGCGCC 1 cut(s) 216
KspI CCGCGG 1 cut(s) 165
LweI GCATC 2 cut(s) 123, 187
MaeI CTAG 2 cut(s) 29, 527
MaeII ACGT 1 cut(s) 456
MaeIII GTNAC 4 cut(s) 262, 542, 565, 658
MboII GAAGA 1 cut(s) 605
MhlI GDGCHC 2 cut(s) 406, 614
MlsI TGGCCA 1 cut(s) 440
MluCI AATT 1 cut(s) 96
MluNI TGGCCA 1 cut(s) 440
Mly113I GGCGCC 1 cut(s) 217
MlyI GAGTC 4 cut(s) 219, 259, 470, 637
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 8 cut(s) 40, 65, 116, 188, 199, 542, 595, 598
Mox20I TGGCCA 1 cut(s) 440
Mph1103I ATGCAT 1 cut(s) 116
MscI TGGCCA 1 cut(s) 440
MseI TTAA 1 cut(s) 3
MslI CAYNNNNRTG 2 cut(s) 12, 420
Msp20I TGGCCA 1 cut(s) 440
MspA1I CMGCKG 3 cut(s) 164, 311, 473
MspI CCGG 1 cut(s) 272
MspR9I CCNGG 2 cut(s) 386, 410
MvaI CCWGG 2 cut(s) 386, 410
MvnI CGCG 1 cut(s) 164
MwoI GCNNNNNNNGC 4 cut(s) 158, 317, 441, 446
NarI GGCGCC 1 cut(s) 217
NdeI CATATG 1 cut(s) 9
NlaIII CATG 1 cut(s) 425
NlaIV GGNNCC 3 cut(s) 16, 218, 504
NmeAIII GCCGAG 1 cut(s) 163
NmuCI GTSAC 4 cut(s) 262, 542, 565, 658
NsiI ATGCAT 1 cut(s) 116
PfeI GAWTC 1 cut(s) 174
PkrI GCNGC 2 cut(s) 163, 221
PleI GAGTC 4 cut(s) 218, 259, 470, 636
PluTI GGCGCC 1 cut(s) 220
PpsI GAGTC 4 cut(s) 218, 259, 470, 636
Ppu21I YACGTR 1 cut(s) 457
PpuMI RGGWCCY 1 cut(s) 502
Psp5II RGGWCCY 1 cut(s) 502
Psp6I CCWGG 2 cut(s) 384, 408
PspFI CCCAGC 1 cut(s) 586
PspGI CCWGG 2 cut(s) 384, 408
PspN4I GGNNCC 3 cut(s) 16, 218, 504
PspPI GGNCC 3 cut(s) 412, 502, 551
PspPPI RGGWCCY 1 cut(s) 502
PvuII CAGCTG 1 cut(s) 473
RsaI GTAC 5 cut(s) 281, 329, 350, 395, 622
RsaNI GTAC 5 cut(s) 280, 328, 349, 394, 621
RseI CAYNNNNRTG 2 cut(s) 12, 420
SacII CCGCGG 1 cut(s) 165
SaqAI TTAA 1 cut(s) 3
SatI GCNGC 2 cut(s) 162, 220
Sau96I GGNCC 3 cut(s) 412, 502, 551
ScaI AGTACT 1 cut(s) 281
SchI GAGTC 4 cut(s) 219, 259, 470, 637
ScrFI CCNGG 2 cut(s) 386, 410
SduI GDGCHC 2 cut(s) 406, 614
SetI ASST 8 cut(s) 67, 108, 246, 390, 459, 475, 563, 726
SfaNI GCATC 2 cut(s) 123, 187
SfcI CTRYAG 1 cut(s) 725
SfoI GGCGCC 1 cut(s) 218
Sfr303I CCGCGG 1 cut(s) 165
SgrBI CCGCGG 1 cut(s) 165
SinI GGWCC 1 cut(s) 502
SmiMI CAYNNNNRTG 2 cut(s) 12, 420
Sse9I AATT 1 cut(s) 96
SsiI CCGC 5 cut(s) 162, 164, 220, 311, 399
SspDI GGCGCC 1 cut(s) 216
SspMI CTAG 2 cut(s) 29, 527
StyD4I CCNGG 2 cut(s) 384, 408
TaaI ACNGT 3 cut(s) 82, 205, 262
TaiI ACGT 1 cut(s) 459
TaqI TCGA 3 cut(s) 117, 208, 720
TasI AATT 1 cut(s) 96
TatI WGTACW 2 cut(s) 279, 620
TauI GCSGC 2 cut(s) 164, 222
TfiI GAWTC 1 cut(s) 174
Tru1I TTAA 1 cut(s) 3
Tru9I TTAA 1 cut(s) 3
TscAI CASTG 3 cut(s) 87, 346, 469
TseFI GTSAC 4 cut(s) 262, 542, 565, 658
Tsp45I GTSAC 4 cut(s) 262, 542, 565, 658
TspRI CASTG 3 cut(s) 87, 346, 469
VneI GTGCAC 1 cut(s) 610
VpaK11BI GGWCC 1 cut(s) 502
XapI RAATTY 1 cut(s) 96
XspI CTAG 2 cut(s) 29, 527
ZrmI AGTACT 1 cut(s) 281
Zsp2I ATGCAT 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.