AT3G22260

OTU domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002298
Physical Location & Seq
Forward (+)
7870826 .. 7873597
2772 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22260.3

Sequence Viewer

Length: 723 bp
ATGGATGAAAACCATAGGAATCCATTTGCAAATGCAAGTACAAGTGCTAGAGCAAGCGGCAGTACAAGTGCGAGCTCAAACTCGAGTTTTAGTAGCAGTGTGGCGGATACAGATGATGATCAGACCATTGCACGTATATTAGCTGAAGATGAGAGCTTGAGAAGAGAAGGCAAGCTTGGGAAGAGATTATCTCATTTGGATTCTATCCCACACACTCCTCGGGTTAACAGGGAGATACCTGATATAAATGATGCAACATTAGACCATGAGCTGCTCTCTGGGAGGTTGGCCACATATGGTTTAGCGGAATTGCAAATGGAGGGAGATGGAAATTGCCAGTTTCGAGCTTTAGCAGACCAGCTTTTCCGCAATGCAGATTACCATAAACATGTAAGGAAGCATGTTGTAAAGCAGTTAAAACAACAACGGAAGTTATACGAAGAATATGTGCCAATGAAATACAGACACTACACCAGGAAGATGAAAAAACATGGTGAATGGGGAGATCATGTTACTCTTCAAGCTGCAGCGGATAGATTCGAAGCCAAGATCTGCTTAGTCACATCCTTTCGGGATCAATCCTACATCGAGATTCTTCCTCATAACAAGAACCCTCTTAGAGAGGCTTGGCTCAGCTTCTGGAGCGAAGTGCATTACAATTCTTTATACGCTAATGGAGATGTTCCAACAAGAAAACCGAGAAGGAAGCATTGGCTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

240

Amino Acids

27.73

Weight (kDa)

8.97

Isoelectric Point (pI)

41.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 108 - 201 2.3e-13 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 57, 104, 305, 367, 530
AclWI GGATC 1 cut(s) 582
AcoI YGGCCR 1 cut(s) 288
AcuI CTGAAG 1 cut(s) 165
AfaI GTAC 2 cut(s) 40, 64
AflIII ACRYGT 1 cut(s) 388
AgsI TTSAA 1 cut(s) 521
AjnI CCWGG 1 cut(s) 473
AjuI GAANNNNNNNTTGG 3 cut(s) 159, 191, 694
AluBI AGCT 9 cut(s) 75, 143, 156, 175, 271, 347, 361, 524, 636
AluI AGCT 9 cut(s) 75, 143, 156, 175, 271, 347, 361, 524, 636
Alw21I GWGCWC 1 cut(s) 77
AlwI GGATC 1 cut(s) 582
AlwNI CAGNNNCTG 1 cut(s) 639
Ama87I CYCGRG 2 cut(s) 82, 219
AoxI GGCC 1 cut(s) 288
ApeKI GCWGC 3 cut(s) 271, 524, 527
AsuHPI GGTGA 1 cut(s) 506
AsuII TTCGAA 1 cut(s) 540
AvaI CYCGRG 2 cut(s) 82, 219
BalI TGGCCA 1 cut(s) 290
BanII GRGCYC 1 cut(s) 77
Bbv12I GWGCWC 1 cut(s) 77
BbvI GCAGC 3 cut(s) 258, 511, 539
BccI CCATC 1 cut(s) 320
BciT130I CCWGG 1 cut(s) 475
BciVI GTATCC 1 cut(s) 100
BclI TGATCA 1 cut(s) 118
BfaI CTAG 2 cut(s) 48, 721
BfmI CTRYAG 1 cut(s) 525
BfuI GTATCC 1 cut(s) 100
BglII AGATCT 1 cut(s) 549
BisI GCNGC 4 cut(s) 58, 272, 525, 528
BlpI GCTNAGC 1 cut(s) 632
BlsI GCNGC 4 cut(s) 59, 273, 526, 529
Bme1390I CCNGG 1 cut(s) 475
BmeT110I CYCGRG 2 cut(s) 82, 219
BmrFI CCNGG 1 cut(s) 475
BmsI GCATC 1 cut(s) 241
BplI GAGNNNNNCTC 4 cut(s) 175, 207, 260, 292
BpmI CTGGAG 1 cut(s) 661
Bpu1102I GCTNAGC 1 cut(s) 632
Bpu14I TTCGAA 1 cut(s) 540
BpuEI CTTGAG 1 cut(s) 178
BsaAI YACGTR 1 cut(s) 134
BsaBI GATNNNNATC 1 cut(s) 117
BsaJI CCNNGG 1 cut(s) 218
BsaXI ACNNNNNCTCC 2 cut(s) 495, 525
Bse1I ACTGG 1 cut(s) 337
Bse3DI GCAATG 2 cut(s) 126, 376
Bse8I GATNNNNATC 1 cut(s) 117
BseBI CCWGG 1 cut(s) 475
BseDI CCNNGG 1 cut(s) 218
BseGI GGATG 2 cut(s) 10, 563
BseJI GATNNNNATC 1 cut(s) 117
BseMI GCAATG 2 cut(s) 126, 376
BseMII CTCAG 1 cut(s) 646
BseNI ACTGG 1 cut(s) 337
BseRI GAGGAG 1 cut(s) 207
BseXI GCAGC 3 cut(s) 258, 511, 539
BshFI GGCC 1 cut(s) 290
BsiHKAI GWGCWC 1 cut(s) 77
BsiHKCI CYCGRG 2 cut(s) 82, 219
BsnI GGCC 1 cut(s) 290
BsoBI CYCGRG 2 cut(s) 82, 219
Bsp119I TTCGAA 1 cut(s) 540
Bsp1286I GDGCHC 1 cut(s) 77
Bsp143I GATC 4 cut(s) 118, 505, 549, 574
Bsp1720I GCTNAGC 1 cut(s) 632
BspACI CCGC 5 cut(s) 57, 104, 305, 367, 530
BspANI GGCC 1 cut(s) 290
BspCNI CTCAG 1 cut(s) 645
BspMAI CTGCAG 1 cut(s) 529
BspPI GGATC 1 cut(s) 582
BspT104I TTCGAA 1 cut(s) 540
BsrDI GCAATG 2 cut(s) 126, 376
BsrI ACTGG 1 cut(s) 337
BssECI CCNNGG 1 cut(s) 218
BssMI GATC 4 cut(s) 118, 505, 549, 574
Bst2UI CCWGG 1 cut(s) 475
Bst6I CTCTTC 3 cut(s) 157, 176, 522
BstBAI YACGTR 1 cut(s) 134
BstBI TTCGAA 1 cut(s) 540
BstC8I GCNNGC 3 cut(s) 55, 73, 173
BstDEI CTNAG 3 cut(s) 556, 617, 632
BstF5I GGATG 2 cut(s) 10, 563
BstKTI GATC 4 cut(s) 121, 508, 552, 577
BstMBI GATC 4 cut(s) 118, 505, 549, 574
BstMWI GCNNNNNNNGC 1 cut(s) 642
BstNI CCWGG 1 cut(s) 475
BstNSI RCATGY 2 cut(s) 392, 404
BstSCI CCNGG 1 cut(s) 473
BstSFI CTRYAG 1 cut(s) 525
BstV1I GCAGC 3 cut(s) 258, 511, 539
BstX2I RGATCY 1 cut(s) 549
BstYI RGATCY 1 cut(s) 549
BsuI GTATCC 1 cut(s) 100
BsuRI GGCC 1 cut(s) 290
BtsCI GGATG 2 cut(s) 10, 563
BtsI GCAGTG 1 cut(s) 103
BtsIMutI CAGTG 1 cut(s) 103
Cac8I GCNNGC 3 cut(s) 55, 73, 173
CaiI CAGNNNCTG 1 cut(s) 639
Csp6I GTAC 2 cut(s) 39, 63
CviAII CATG 5 cut(s) 266, 389, 401, 491, 509
CviQI GTAC 2 cut(s) 39, 63
DdeI CTNAG 3 cut(s) 556, 617, 632
DpnI GATC 4 cut(s) 120, 507, 551, 576
DpnII GATC 4 cut(s) 118, 505, 549, 574
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 3 cut(s) 157, 176, 522
EarI CTCTTC 3 cut(s) 157, 176, 522
EciI GGCGGA 1 cut(s) 119
Ecl136II GAGCTC 1 cut(s) 75
Eco24I GRGCYC 1 cut(s) 77
Eco53kI GAGCTC 1 cut(s) 75
Eco57I CTGAAG 1 cut(s) 165
Eco88I CYCGRG 2 cut(s) 82, 219
EcoICRI GAGCTC 1 cut(s) 75
EcoRII CCWGG 1 cut(s) 473
EcoT38I GRGCYC 1 cut(s) 77
FaeI CATG 5 cut(s) 269, 392, 404, 494, 512
FalI AAGNNNNNCTT 4 cut(s) 159, 191, 539, 571
FatI CATG 5 cut(s) 265, 388, 400, 490, 508
FauNDI CATATG 1 cut(s) 295
FbaI TGATCA 1 cut(s) 118
Fnu4HI GCNGC 4 cut(s) 58, 272, 525, 528
FokI GGATG 2 cut(s) 17, 550
FriOI GRGCYC 1 cut(s) 77
Fsp4HI GCNGC 4 cut(s) 58, 272, 525, 528
FspBI CTAG 2 cut(s) 48, 721
GluI GCNGC 4 cut(s) 58, 272, 525, 528
GsuI CTGGAG 1 cut(s) 661
HaeIII GGCC 1 cut(s) 290
Hin1II CATG 5 cut(s) 269, 392, 404, 494, 512
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 4 cut(s) 19, 200, 537, 592
HpaI GTTAAC 1 cut(s) 226
HphI GGTGA 1 cut(s) 506
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 1 cut(s) 123
Hpy188III TCNNGA 3 cut(s) 572, 589, 640
Hpy8I GTNNAC 1 cut(s) 226
HpyAV CCTTC 2 cut(s) 161, 696
HpyCH4IV ACGT 1 cut(s) 133
HpyCH4V TGCA 8 cut(s) 29, 35, 131, 254, 313, 374, 527, 652
HpyF10VI GCNNNNNNNGC 1 cut(s) 642
HpyF3I CTNAG 3 cut(s) 556, 617, 632
HpySE526I ACGT 1 cut(s) 133
Hsp92II CATG 5 cut(s) 269, 392, 404, 494, 512
Ksp22I TGATCA 1 cut(s) 118
KspAI GTTAAC 1 cut(s) 226
Kzo9I GATC 4 cut(s) 118, 505, 549, 574
LmnI GCTCC 1 cut(s) 642
LpnPI CCDG 8 cut(s) 214, 252, 264, 350, 371, 460, 487, 625
Lsp1109I GCAGC 3 cut(s) 258, 511, 539
LweI GCATC 1 cut(s) 241
MaeI CTAG 2 cut(s) 48, 721
MaeII ACGT 1 cut(s) 133
MaeIII GTNAC 2 cut(s) 511, 559
MalI GATC 4 cut(s) 120, 507, 551, 576
MboI GATC 4 cut(s) 118, 505, 549, 574
MboII GAAGA 8 cut(s) 158, 174, 193, 452, 490, 509, 587, 709
MflI RGATCY 1 cut(s) 549
MhlI GDGCHC 1 cut(s) 77
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 3 cut(s) 308, 331, 658
MluNI TGGCCA 1 cut(s) 290
MmeI TCCRAC 1 cut(s) 710
MnlI CCTC 6 cut(s) 228, 276, 313, 609, 616, 624
Mox20I TGGCCA 1 cut(s) 290
MscI TGGCCA 1 cut(s) 290
MseI TTAA 2 cut(s) 225, 416
MslI CAYNNNNRTG 1 cut(s) 387
Msp20I TGGCCA 1 cut(s) 290
MspA1I CMGCKG 1 cut(s) 530
MspR9I CCNGG 1 cut(s) 475
MvaI CCWGG 1 cut(s) 475
MwoI GCNNNNNNNGC 1 cut(s) 642
NdeI CATATG 1 cut(s) 295
NdeII GATC 4 cut(s) 118, 505, 549, 574
NlaIII CATG 5 cut(s) 269, 392, 404, 494, 512
NmuCI GTSAC 1 cut(s) 559
NspI RCATGY 2 cut(s) 392, 404
NspV TTCGAA 1 cut(s) 540
PaeR7I CTCGAG 1 cut(s) 82
PciI ACATGT 1 cut(s) 388
PfeI GAWTC 4 cut(s) 19, 200, 537, 592
PkrI GCNGC 4 cut(s) 59, 273, 526, 529
Ppu21I YACGTR 1 cut(s) 134
PscI ACATGT 1 cut(s) 388
Psp124BI GAGCTC 1 cut(s) 77
Psp6I CCWGG 1 cut(s) 473
PspGI CCWGG 1 cut(s) 473
PspXI VCTCGAGB 1 cut(s) 82
PstI CTGCAG 1 cut(s) 529
PstNI CAGNNNCTG 1 cut(s) 639
PsuI RGATCY 1 cut(s) 549
RsaI GTAC 2 cut(s) 40, 64
RsaNI GTAC 2 cut(s) 39, 63
RseI CAYNNNNRTG 1 cut(s) 387
SacI GAGCTC 1 cut(s) 77
SaqAI TTAA 2 cut(s) 225, 416
SatI GCNGC 4 cut(s) 58, 272, 525, 528
Sau3AI GATC 4 cut(s) 118, 505, 549, 574
ScrFI CCNGG 1 cut(s) 475
SduI GDGCHC 1 cut(s) 77
SfaNI GCATC 1 cut(s) 241
SfcI CTRYAG 1 cut(s) 525
Sfr274I CTCGAG 1 cut(s) 82
SfuI TTCGAA 1 cut(s) 540
SlaI CTCGAG 1 cut(s) 82
SmiMI CAYNNNNRTG 1 cut(s) 387
SmlI CTYRAG 2 cut(s) 82, 157
SmoI CTYRAG 2 cut(s) 82, 157
Sse9I AATT 3 cut(s) 308, 331, 658
SsiI CCGC 5 cut(s) 57, 104, 305, 367, 530
SspMI CTAG 2 cut(s) 48, 721
SstI GAGCTC 1 cut(s) 77
StyD4I CCNGG 1 cut(s) 473
TaiI ACGT 1 cut(s) 136
TaqI TCGA 4 cut(s) 83, 343, 540, 588
TasI AATT 3 cut(s) 308, 331, 658
TatI WGTACW 2 cut(s) 38, 62
TauI GCSGC 1 cut(s) 60
TfiI GAWTC 4 cut(s) 19, 200, 537, 592
Tru1I TTAA 2 cut(s) 225, 416
Tru9I TTAA 2 cut(s) 225, 416
TscAI CASTG 1 cut(s) 103
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 3 cut(s) 271, 524, 527
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 3 cut(s) 21, 470, 497
TspGWI ACGGA 1 cut(s) 442
TspRI CASTG 1 cut(s) 103
XceI RCATGY 2 cut(s) 392, 404
XhoI CTCGAG 1 cut(s) 82
XspI CTAG 2 cut(s) 48, 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.