MD17G1127700.v1.1

OTU domain-containing protein DDB_G0284757-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002298
Physical Location & Seq
Reverse (-)
11192466 .. 11196551
4086 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1127700.v1.1.491

Sequence Viewer

Length: 684 bp
ATGAACGGAAACTACAGCAATGCAAGTGCGAGTTCCAGCTCGAGTTTAGATAGCAGCACTCACGGTACGGAGGATGATCAAACCATTGCAATCATCTTGGGAGAAGAGGAAAAGCTGAAATTTGATGGCACGCTTGGGAAAAGACTGTGCCACTTAAATTCGATTCCGCACACTCCCCGGGTAAATGGGGAGATACCTGATGTGAATGATGCAACCCAAGACCATGAGCGCCTGTCAGCGAGGCTGGCAACATATGATTTATCTGAACTGCAGATCAAGGGCGATGGAAACTGTCAGTTTCGAGCATTAGCAGATCAATTGTTTCGGAATCCAGAATACCACAAGCATGTAAGAAAACAGGTGATAAAGCAGCTAAAGCATCACAAAAAACTTTACGAAGCTTATGTCCCAATGAAGTACAGTCGCTACCTGATGAAGATGAAAAAAACCGGGGAGTGGGGAGATCATCTTACACTACAGGCAGCTGCAGATCGATTCGGAGCCAAGATTTGTTTAATCACCTCTTTTCGGGACACTTGCTACATCGAGATTCTTCCTAAAGATGGGAATCATGCGCGAGAGCTTTGGCTGAGCTTTTGGAGCGAAGTGCATTATAACTCCTTGTATGCAAGTGCAGATGTTCCGTCAAGAACTCCCCGAAAGAAGCATTGGCTGTCATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

228

Amino Acids

26.01

Weight (kDa)

8.56

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 93 - 201 3.4e-13 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 615
AccII CGCG 1 cut(s) 577
AciI CCGC 1 cut(s) 167
AcsI RAATTY 2 cut(s) 119, 157
AfaI GTAC 2 cut(s) 67, 419
AfiI CCNNNNNNNGG 3 cut(s) 456, 528, 563
AjuI GAANNNNNNNTTGG 2 cut(s) 652, 684
AluBI AGCT 7 cut(s) 39, 115, 373, 401, 485, 583, 594
AluI AGCT 7 cut(s) 39, 115, 373, 401, 485, 583, 594
Ama87I CYCGRG 2 cut(s) 40, 177
ApeKI GCWGC 4 cut(s) 54, 370, 482, 485
ApoI RAATTY 2 cut(s) 119, 157
ArsI GACNNNNNNTTYG 2 cut(s) 390, 422
AspLEI GCGC 2 cut(s) 231, 577
AsuC2I CCSGG 3 cut(s) 178, 179, 451
AsuHPI GGTGA 2 cut(s) 373, 511
AvaI CYCGRG 2 cut(s) 40, 177
BbvI GCAGC 4 cut(s) 66, 382, 472, 494
BccI CCATC 3 cut(s) 119, 278, 557
BclI TGATCA 1 cut(s) 76
BcnI CCSGG 3 cut(s) 178, 179, 451
BfaI CTAG 1 cut(s) 682
BfmI CTRYAG 4 cut(s) 13, 269, 476, 486
BfoI RGCGCY 1 cut(s) 232
BisI GCNGC 4 cut(s) 55, 371, 483, 486
BlpI GCTNAGC 1 cut(s) 590
BlsI GCNGC 4 cut(s) 56, 372, 484, 487
Bme1390I CCNGG 3 cut(s) 178, 179, 451
BmeT110I CYCGRG 2 cut(s) 40, 177
BmiI GGNNCC 1 cut(s) 502
BmrFI CCNGG 3 cut(s) 178, 179, 451
BmsI GCATC 2 cut(s) 199, 388
Bpu1102I GCTNAGC 1 cut(s) 590
BpuMI CCSGG 3 cut(s) 178, 179, 451
Bsa29I ATCGAT 1 cut(s) 493
BsaBI GATNNNNATC 1 cut(s) 567
BsaJI CCNNGG 3 cut(s) 176, 177, 450
Bsc4I CCNNNNNNNGG 3 cut(s) 456, 528, 563
Bse3DI GCAATG 2 cut(s) 25, 84
Bse8I GATNNNNATC 1 cut(s) 567
BseCI ATCGAT 1 cut(s) 493
BseDI CCNNGG 3 cut(s) 176, 177, 450
BseGI GGATG 1 cut(s) 79
BseJI GATNNNNATC 1 cut(s) 567
BseLI CCNNNNNNNGG 3 cut(s) 456, 528, 563
BseMI GCAATG 2 cut(s) 25, 84
BseMII CTCAG 1 cut(s) 581
BseXI GCAGC 4 cut(s) 66, 382, 472, 494
BsgI GTGCAG 1 cut(s) 654
Bsh1236I CGCG 1 cut(s) 577
BshVI ATCGAT 1 cut(s) 493
BsiHKCI CYCGRG 2 cut(s) 40, 177
BsiSI CCGG 2 cut(s) 178, 450
BslFI GGGAC 2 cut(s) 392, 545
BslI CCNNNNNNNGG 3 cut(s) 456, 528, 563
BsmFI GGGAC 2 cut(s) 392, 545
BsoBI CYCGRG 2 cut(s) 40, 177
Bsp143I GATC 5 cut(s) 76, 273, 313, 463, 490
Bsp1720I GCTNAGC 1 cut(s) 590
BspACI CCGC 1 cut(s) 167
BspCNI CTCAG 1 cut(s) 582
BspDI ATCGAT 1 cut(s) 493
BspFNI CGCG 1 cut(s) 577
BspLI GGNNCC 1 cut(s) 502
BspMAI CTGCAG 2 cut(s) 273, 490
BsrDI GCAATG 2 cut(s) 25, 84
BssECI CCNNGG 3 cut(s) 176, 177, 450
BssMI GATC 5 cut(s) 76, 273, 313, 463, 490
Bst4CI ACNGT 4 cut(s) 65, 147, 293, 422
Bst6I CTCTTC 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 131, 246
BstDEI CTNAG 1 cut(s) 590
BstF5I GGATG 1 cut(s) 79
BstFNI CGCG 1 cut(s) 577
BstH2I RGCGCY 1 cut(s) 232
BstHHI GCGC 2 cut(s) 231, 577
BstKTI GATC 5 cut(s) 79, 276, 316, 466, 493
BstMBI GATC 5 cut(s) 76, 273, 313, 463, 490
BstMWI GCNNNNNNNGC 3 cut(s) 245, 376, 600
BstNSI RCATGY 1 cut(s) 350
BstSCI CCNGG 3 cut(s) 176, 177, 449
BstSFI CTRYAG 4 cut(s) 13, 269, 476, 486
BstUI CGCG 1 cut(s) 577
BstV1I GCAGC 4 cut(s) 66, 382, 472, 494
Bsu15I ATCGAT 1 cut(s) 493
BsuTUI ATCGAT 1 cut(s) 493
BtgZI GCGATG 1 cut(s) 297
BtsCI GGATG 1 cut(s) 79
Cac8I GCNNGC 2 cut(s) 131, 246
CfoI GCGC 2 cut(s) 231, 577
Cfr9I CCCGGG 1 cut(s) 177
ClaI ATCGAT 1 cut(s) 493
Csp6I GTAC 2 cut(s) 66, 418
CviAII CATG 3 cut(s) 224, 347, 572
CviQI GTAC 2 cut(s) 66, 418
DdeI CTNAG 1 cut(s) 590
DpnI GATC 5 cut(s) 78, 275, 315, 465, 492
DpnII GATC 5 cut(s) 76, 273, 313, 463, 490
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Eco88I CYCGRG 2 cut(s) 40, 177
FaeI CATG 3 cut(s) 227, 350, 575
FaiI YATR 8 cut(s) 225, 253, 255, 348, 405, 573, 615, 627
FaqI GGGAC 2 cut(s) 392, 545
FatI CATG 3 cut(s) 223, 346, 571
FauNDI CATATG 1 cut(s) 253
FbaI TGATCA 1 cut(s) 76
Fnu4HI GCNGC 4 cut(s) 55, 371, 483, 486
FokI GGATG 1 cut(s) 86
Fsp4HI GCNGC 4 cut(s) 55, 371, 483, 486
FspBI CTAG 1 cut(s) 682
GlaI GCGC 2 cut(s) 230, 576
GluI GCNGC 4 cut(s) 55, 371, 483, 486
HaeII RGCGCY 1 cut(s) 232
HapII CCGG 2 cut(s) 178, 450
HhaI GCGC 2 cut(s) 231, 577
Hin1II CATG 3 cut(s) 227, 350, 575
Hin6I GCGC 2 cut(s) 229, 575
HinP1I GCGC 2 cut(s) 229, 575
HindIII AAGCTT 1 cut(s) 399
HinfI GANTC 5 cut(s) 163, 328, 495, 550, 568
HpaII CCGG 2 cut(s) 178, 450
HphI GGTGA 2 cut(s) 373, 511
Hpy188I TCNGA 3 cut(s) 265, 327, 500
Hpy188III TCNNGA 4 cut(s) 332, 530, 547, 648
HpyCH4III ACNGT 4 cut(s) 65, 147, 293, 422
HpyCH4V TGCA 8 cut(s) 23, 89, 212, 271, 488, 610, 629, 635
HpyF10VI GCNNNNNNNGC 3 cut(s) 245, 376, 600
HpyF3I CTNAG 1 cut(s) 590
Hsp92II CATG 3 cut(s) 227, 350, 575
HspAI GCGC 2 cut(s) 229, 575
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 5 cut(s) 76, 273, 313, 463, 490
LmnI GCTCC 2 cut(s) 500, 600
Lsp1109I GCAGC 4 cut(s) 66, 382, 472, 494
LweI GCATC 2 cut(s) 199, 388
MaeI CTAG 1 cut(s) 682
MalI GATC 5 cut(s) 78, 275, 315, 465, 492
MboI GATC 5 cut(s) 76, 273, 313, 463, 490
MboII GAAGA 3 cut(s) 116, 448, 545
MfeI CAATTG 1 cut(s) 317
MluCI AATT 3 cut(s) 119, 157, 317
MnlI CCTC 4 cut(s) 64, 100, 234, 532
MseI TTAA 2 cut(s) 155, 515
MslI CAYNNNNRTG 1 cut(s) 345
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 2 cut(s) 178, 450
MspR9I CCNGG 3 cut(s) 178, 179, 451
MunI CAATTG 1 cut(s) 317
MvnI CGCG 1 cut(s) 577
MwoI GCNNNNNNNGC 3 cut(s) 245, 376, 600
NciI CCSGG 3 cut(s) 178, 179, 451
NdeI CATATG 1 cut(s) 253
NdeII GATC 5 cut(s) 76, 273, 313, 463, 490
NlaIII CATG 3 cut(s) 227, 350, 575
NlaIV GGNNCC 1 cut(s) 502
NspI RCATGY 1 cut(s) 350
PaeR7I CTCGAG 1 cut(s) 40
PfeI GAWTC 5 cut(s) 163, 328, 495, 550, 568
PkrI GCNGC 4 cut(s) 56, 372, 484, 487
PsiI TTATAA 1 cut(s) 615
PspN4I GGNNCC 1 cut(s) 502
PspXI VCTCGAGB 1 cut(s) 40
PsrI GAACNNNNNNTAC 1 cut(s) 28
PstI CTGCAG 2 cut(s) 273, 490
PvuII CAGCTG 1 cut(s) 485
RsaI GTAC 2 cut(s) 67, 419
RsaNI GTAC 2 cut(s) 66, 418
RseI CAYNNNNRTG 1 cut(s) 345
SaqAI TTAA 2 cut(s) 155, 515
SatI GCNGC 4 cut(s) 55, 371, 483, 486
Sau3AI GATC 5 cut(s) 76, 273, 313, 463, 490
ScrFI CCNGG 3 cut(s) 178, 179, 451
SfaNI GCATC 2 cut(s) 199, 388
SfcI CTRYAG 4 cut(s) 13, 269, 476, 486
Sfr274I CTCGAG 1 cut(s) 40
SlaI CTCGAG 1 cut(s) 40
SmaI CCCGGG 1 cut(s) 179
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 1 cut(s) 40
SmoI CTYRAG 1 cut(s) 40
Sse9I AATT 3 cut(s) 119, 157, 317
SsiI CCGC 1 cut(s) 167
SspMI CTAG 1 cut(s) 682
StyD4I CCNGG 3 cut(s) 176, 177, 449
TaaI ACNGT 4 cut(s) 65, 147, 293, 422
TaqI TCGA 5 cut(s) 41, 161, 301, 493, 546
TasI AATT 3 cut(s) 119, 157, 317
TatI WGTACW 1 cut(s) 417
TfiI GAWTC 5 cut(s) 163, 328, 495, 550, 568
Tru1I TTAA 2 cut(s) 155, 515
Tru9I TTAA 2 cut(s) 155, 515
TseI GCWGC 4 cut(s) 54, 370, 482, 485
TspDTI ATGAA 4 cut(s) 17, 428, 449, 455
TspGWI ACGGA 3 cut(s) 21, 83, 633
TspMI CCCGGG 1 cut(s) 177
XapI RAATTY 2 cut(s) 119, 157
XceI RCATGY 1 cut(s) 350
XhoI CTCGAG 1 cut(s) 40
XmaI CCCGGG 1 cut(s) 177
XspI CTAG 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.