Rh2DG527900

OTU domain-containing protein DDB_G0284757-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002298
Physical Location & Seq
Forward (+)
75331876 .. 75336344
4469 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG527900.1

Sequence Viewer

Length: 474 bp
ATGAATGGAAGCTATAGCAATGCAAGTGCGAGCTCAAGCTCGAGTTTGAATAGCAGCTCTCATGATACCGAGGATGACCTGACCATTGCAACTGTCTTGGCAGAAGAGGAAAAGCAGAAAAGTGATGGCAGGCTGGGGAAAAGACTCTCCCACTTAGATTCGATTCCGCACACTCCCAGGGTGAATGGGGAGATACCTGACGTGAATGATGCTACTCAGGACCATAAGAGGCTTTCAGAAAGGCTGGCTACCTATGATCTATCTGAGCTGCAAATTGAGGGTGATGGAAATTGCCAGTTTCGAGCAATAGCTGATCAGTTGTTTCGCAATCCGGATTACCATAAGCATGTTAGAAAGCAGGTTATAAAGCAGTTAAAGCATCACAGAAAACTCTATGAAGCTTATGTCCCAATGAAATACAAAAGTTACCTGAGGAAGATGAAAAAGTTAGATATCTTCTTTTATGCCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

157

Amino Acids

18.09

Weight (kDa)

8.55

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 94 - 149 5.7e-06 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 365
Acc36I ACCTGC 1 cut(s) 349
AccIII TCCGGA 1 cut(s) 331
AciI CCGC 1 cut(s) 167
AgsI TTSAA 1 cut(s) 49
AjiI CACGTC 1 cut(s) 202
AjnI CCWGG 1 cut(s) 176
AluBI AGCT 7 cut(s) 12, 33, 39, 57, 268, 311, 401
AluI AGCT 7 cut(s) 12, 33, 39, 57, 268, 311, 401
Alw21I GWGCWC 1 cut(s) 35
Ama87I CYCGRG 1 cut(s) 40
Aor13HI TCCGGA 1 cut(s) 331
ApeKI GCWGC 2 cut(s) 54, 268
AspS9I GGNCC 1 cut(s) 220
AsuHPI GGTGA 2 cut(s) 193, 293
AvaI CYCGRG 1 cut(s) 40
AvaII GGWCC 1 cut(s) 220
AxyI CCTNAGG 1 cut(s) 431
BanII GRGCYC 1 cut(s) 35
Bbv12I GWGCWC 1 cut(s) 35
BbvI GCAGC 2 cut(s) 66, 255
BccI CCATC 2 cut(s) 119, 278
BciT130I CCWGG 1 cut(s) 178
BclI TGATCA 1 cut(s) 313
BfmI CTRYAG 1 cut(s) 13
BfuAI ACCTGC 1 cut(s) 349
BisI GCNGC 2 cut(s) 55, 269
BlsI GCNGC 2 cut(s) 56, 270
Bme1390I CCNGG 1 cut(s) 178
Bme18I GGWCC 1 cut(s) 220
BmeT110I CYCGRG 1 cut(s) 40
BmgBI CACGTC 1 cut(s) 202
BmgT120I GGNCC 1 cut(s) 220
BmrFI CCNGG 1 cut(s) 178
BmsI GCATC 2 cut(s) 199, 388
BpuEI CTTGAG 1 cut(s) 19
BsaJI CCNNGG 3 cut(s) 69, 176, 177
BsaWI WCCGGW 1 cut(s) 331
Bse1I ACTGG 1 cut(s) 295
Bse21I CCTNAGG 1 cut(s) 431
Bse3DI GCAATG 2 cut(s) 25, 84
BseAI TCCGGA 1 cut(s) 331
BseBI CCWGG 1 cut(s) 178
BseDI CCNNGG 3 cut(s) 69, 176, 177
BseGI GGATG 1 cut(s) 79
BseMI GCAATG 2 cut(s) 25, 84
BseMII CTCAG 3 cut(s) 230, 255, 422
BseNI ACTGG 1 cut(s) 295
BseXI GCAGC 2 cut(s) 66, 255
BseYI CCCAGC 1 cut(s) 133
BsiHKAI GWGCWC 1 cut(s) 35
BsiHKCI CYCGRG 1 cut(s) 40
BsiSI CCGG 1 cut(s) 332
BslFI GGGAC 1 cut(s) 392
BsmFI GGGAC 1 cut(s) 392
BsoBI CYCGRG 1 cut(s) 40
Bsp1286I GDGCHC 1 cut(s) 35
Bsp13I TCCGGA 1 cut(s) 331
Bsp143I GATC 2 cut(s) 256, 313
BspACI CCGC 1 cut(s) 167
BspCNI CTCAG 3 cut(s) 229, 256, 423
BspEI TCCGGA 1 cut(s) 331
BspHI TCATGA 1 cut(s) 61
BspMI ACCTGC 1 cut(s) 349
BsrDI GCAATG 2 cut(s) 25, 84
BsrI ACTGG 1 cut(s) 295
BssECI CCNNGG 3 cut(s) 69, 176, 177
BssMI GATC 2 cut(s) 256, 313
Bst2UI CCWGG 1 cut(s) 178
Bst4CI ACNGT 1 cut(s) 94
Bst6I CTCTTC 1 cut(s) 99
BstC8I GCNNGC 3 cut(s) 31, 131, 246
BstDEI CTNAG 4 cut(s) 154, 216, 264, 431
BstF5I GGATG 1 cut(s) 79
BstKTI GATC 2 cut(s) 259, 316
BstMBI GATC 2 cut(s) 256, 313
BstMWI GCNNNNNNNGC 1 cut(s) 376
BstNI CCWGG 1 cut(s) 178
BstNSI RCATGY 1 cut(s) 350
BstSCI CCNGG 1 cut(s) 176
BstSFI CTRYAG 1 cut(s) 13
BstV1I GCAGC 2 cut(s) 66, 255
Bsu36I CCTNAGG 1 cut(s) 431
BtrI CACGTC 1 cut(s) 202
BtsCI GGATG 1 cut(s) 79
BveI ACCTGC 1 cut(s) 349
Cac8I GCNNGC 3 cut(s) 31, 131, 246
CciI TCATGA 1 cut(s) 61
Cfr13I GGNCC 1 cut(s) 220
CviAII CATG 2 cut(s) 62, 347
DdeI CTNAG 4 cut(s) 154, 216, 264, 431
DpnI GATC 2 cut(s) 258, 315
DpnII GATC 2 cut(s) 256, 313
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Ecl136II GAGCTC 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 35
Eco32I GATATC 1 cut(s) 454
Eco47I GGWCC 1 cut(s) 220
Eco53kI GAGCTC 1 cut(s) 33
Eco81I CCTNAGG 1 cut(s) 431
Eco88I CYCGRG 1 cut(s) 40
EcoICRI GAGCTC 1 cut(s) 33
EcoRII CCWGG 1 cut(s) 176
EcoRV GATATC 1 cut(s) 454
EcoT38I GRGCYC 1 cut(s) 35
FaeI CATG 2 cut(s) 65, 350
FaqI GGGAC 1 cut(s) 392
FatI CATG 2 cut(s) 61, 346
FbaI TGATCA 1 cut(s) 313
Fnu4HI GCNGC 2 cut(s) 55, 269
FokI GGATG 1 cut(s) 86
FriOI GRGCYC 1 cut(s) 35
Fsp4HI GCNGC 2 cut(s) 55, 269
GluI GCNGC 2 cut(s) 55, 269
GsaI CCCAGC 1 cut(s) 137
HapII CCGG 1 cut(s) 332
Hin1II CATG 2 cut(s) 65, 350
HindIII AAGCTT 1 cut(s) 399
HinfI GANTC 3 cut(s) 144, 158, 163
HpaII CCGG 1 cut(s) 332
HphI GGTGA 2 cut(s) 193, 293
Hpy188I TCNGA 2 cut(s) 238, 265
Hpy188III TCNNGA 3 cut(s) 62, 218, 332
HpyCH4III ACNGT 1 cut(s) 94
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 3 cut(s) 23, 89, 271
HpyF10VI GCNNNNNNNGC 1 cut(s) 376
HpyF3I CTNAG 4 cut(s) 154, 216, 264, 431
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 2 cut(s) 65, 350
Kpn2I TCCGGA 1 cut(s) 331
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 2 cut(s) 256, 313
Lsp1109I GCAGC 2 cut(s) 66, 255
LweI GCATC 2 cut(s) 199, 388
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 1 cut(s) 425
MalI GATC 2 cut(s) 258, 315
MboI GATC 2 cut(s) 256, 313
MboII GAAGA 3 cut(s) 116, 448, 448
MhlI GDGCHC 1 cut(s) 35
MluCI AATT 2 cut(s) 273, 289
MlyI GAGTC 1 cut(s) 138
MnlI CCTC 5 cut(s) 64, 100, 222, 271, 426
MroI TCCGGA 1 cut(s) 331
MseI TTAA 1 cut(s) 374
MslI CAYNNNNRTG 1 cut(s) 345
MspI CCGG 1 cut(s) 332
MspR9I CCNGG 1 cut(s) 178
MvaI CCWGG 1 cut(s) 178
MwoI GCNNNNNNNGC 1 cut(s) 376
NdeII GATC 2 cut(s) 256, 313
NlaIII CATG 2 cut(s) 65, 350
NspI RCATGY 1 cut(s) 350
PaeR7I CTCGAG 1 cut(s) 40
PagI TCATGA 1 cut(s) 61
PasI CCCWGGG 1 cut(s) 177
PfeI GAWTC 2 cut(s) 158, 163
PkrI GCNGC 2 cut(s) 56, 270
PleI GAGTC 1 cut(s) 138
PpsI GAGTC 1 cut(s) 138
PsiI TTATAA 1 cut(s) 365
Psp124BI GAGCTC 1 cut(s) 35
Psp6I CCWGG 1 cut(s) 176
PspFI CCCAGC 1 cut(s) 133
PspGI CCWGG 1 cut(s) 176
PspPI GGNCC 1 cut(s) 220
PspXI VCTCGAGB 1 cut(s) 40
RseI CAYNNNNRTG 1 cut(s) 345
SacI GAGCTC 1 cut(s) 35
SaqAI TTAA 1 cut(s) 374
SatI GCNGC 2 cut(s) 55, 269
Sau3AI GATC 2 cut(s) 256, 313
Sau96I GGNCC 1 cut(s) 220
SchI GAGTC 1 cut(s) 138
ScrFI CCNGG 1 cut(s) 178
SduI GDGCHC 1 cut(s) 35
SfaNI GCATC 2 cut(s) 199, 388
SfcI CTRYAG 1 cut(s) 13
Sfr274I CTCGAG 1 cut(s) 40
SinI GGWCC 1 cut(s) 220
SlaI CTCGAG 1 cut(s) 40
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 2 cut(s) 34, 40
SmoI CTYRAG 2 cut(s) 34, 40
Sse9I AATT 2 cut(s) 273, 289
SsiI CCGC 1 cut(s) 167
SstI GAGCTC 1 cut(s) 35
StyD4I CCNGG 1 cut(s) 176
TaaI ACNGT 1 cut(s) 94
TaiI ACGT 1 cut(s) 204
TaqI TCGA 3 cut(s) 41, 161, 301
TasI AATT 2 cut(s) 273, 289
TfiI GAWTC 2 cut(s) 158, 163
Tru1I TTAA 1 cut(s) 374
Tru9I TTAA 1 cut(s) 374
TseI GCWGC 2 cut(s) 54, 268
TspDTI ATGAA 4 cut(s) 17, 411, 428, 455
VpaK11BI GGWCC 1 cut(s) 220
XceI RCATGY 1 cut(s) 350
XhoI CTCGAG 1 cut(s) 40
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.