MD09G1137400.v1.1

OTU domain-containing protein DDB_G0284757-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002298
Physical Location & Seq
Reverse (-)
10638909 .. 10643350
4442 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1137400.v1.1.491

Sequence Viewer

Length: 684 bp
ATGAGTGGAAGCTACAGCAATGCGAGTGCGAGTTCAAGCTCGAGTTTAGATAGCAGCAACCATGATACAGAGGATGATCAAACCATTGCAACCATGTTGGCAGAAGAGGAAAAGGTCAAAGTTGATCGCAGGCTTGGGAAGAGACTGTCCCACTTAGATTCGATTCCGCACACTCCCCGCGTAAATGGGGAGATACCTGACGTGAATGATGCGACCCAAGACCATGAGCGCCTGTCGGCAAGGTTGGCAACGTATGATTTATCTGAACTGCAGATCGAGGGCGATGGAAATTGTCAGTTTCGAGCAATAGCAGATCAATTGTTTCGGAATCCAGAATACCACAAGCATGTAAGAAAACAGGTGATAAAGCAGCTAAAGCATCACAAAAAACTCTATGAAGCTTATGTCCCGATGAAATACAGACGCTACCTGAGGAAGATGAAAAAAACCGGGGAGTGGGGAGATCATCTTACGCTACAGGCAGCTGCAGATCGATTCGGAGCCAAGATTTGTTTAATCACCTCTTTTCGAGACACTTGCTTCATCGAGATTCTTCCTAAAGATGGGAATCCTACCCTAGAGCTTTGGCTGAGCTTTTGGAGTGAAGTGCATTATAACTCCTTGTATGCAAGTGCAGATGTTCCGACTAGAACTCCCCGAAAGAAGCATTGGCTGTTATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

228

Amino Acids

26.19

Weight (kDa)

8.34

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 94 - 182 4.4e-14 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 615
AccII CGCG 1 cut(s) 180
AciI CCGC 2 cut(s) 167, 178
AfiI CCNNNNNNNGG 2 cut(s) 456, 563
AgsI TTSAA 1 cut(s) 36
AjiI CACGTC 1 cut(s) 202
AjuI GAANNNNNNNTTGG 2 cut(s) 652, 684
AluBI AGCT 7 cut(s) 12, 39, 373, 401, 485, 583, 594
AluI AGCT 7 cut(s) 12, 39, 373, 401, 485, 583, 594
Alw26I GTCTC 2 cut(s) 136, 525
Ama87I CYCGRG 1 cut(s) 40
ApeKI GCWGC 4 cut(s) 54, 370, 482, 485
AspLEI GCGC 1 cut(s) 231
AsuC2I CCSGG 1 cut(s) 451
AsuHPI GGTGA 2 cut(s) 373, 511
AvaI CYCGRG 1 cut(s) 40
AxyI CCTNAGG 1 cut(s) 431
BbvI GCAGC 4 cut(s) 66, 382, 472, 494
BccI CCATC 2 cut(s) 278, 557
BcgI CGANNNNNNTGC 2 cut(s) 519, 553
BclI TGATCA 1 cut(s) 76
BcnI CCSGG 1 cut(s) 451
BcoDI GTCTC 2 cut(s) 136, 525
BfaI CTAG 3 cut(s) 578, 648, 682
BfmI CTRYAG 4 cut(s) 13, 269, 476, 486
BfoI RGCGCY 1 cut(s) 232
BisI GCNGC 4 cut(s) 55, 371, 483, 486
BlpI GCTNAGC 1 cut(s) 590
BlsI GCNGC 4 cut(s) 56, 372, 484, 487
Bme1390I CCNGG 1 cut(s) 451
BmeT110I CYCGRG 1 cut(s) 40
BmgBI CACGTC 1 cut(s) 202
BmiI GGNNCC 1 cut(s) 502
BmrFI CCNGG 1 cut(s) 451
BmsI GCATC 2 cut(s) 199, 388
Bpu1102I GCTNAGC 1 cut(s) 590
BpuMI CCSGG 1 cut(s) 451
Bsa29I ATCGAT 1 cut(s) 493
BsaBI GATNNNNATC 1 cut(s) 567
BsaJI CCNNGG 1 cut(s) 450
BsaXI ACNNNNNCTCC 2 cut(s) 637, 667
Bsc4I CCNNNNNNNGG 2 cut(s) 456, 563
Bse21I CCTNAGG 1 cut(s) 431
Bse3DI GCAATG 2 cut(s) 25, 84
Bse8I GATNNNNATC 1 cut(s) 567
BseCI ATCGAT 1 cut(s) 493
BseDI CCNNGG 1 cut(s) 450
BseGI GGATG 1 cut(s) 79
BseJI GATNNNNATC 1 cut(s) 567
BseLI CCNNNNNNNGG 2 cut(s) 456, 563
BseMI GCAATG 2 cut(s) 25, 84
BseMII CTCAG 2 cut(s) 422, 581
BseXI GCAGC 4 cut(s) 66, 382, 472, 494
BsgI GTGCAG 1 cut(s) 654
Bsh1236I CGCG 1 cut(s) 180
BshVI ATCGAT 1 cut(s) 493
BsiHKCI CYCGRG 1 cut(s) 40
BsiSI CCGG 1 cut(s) 450
BslFI GGGAC 2 cut(s) 133, 392
BslI CCNNNNNNNGG 2 cut(s) 456, 563
BsmAI GTCTC 2 cut(s) 136, 525
BsmFI GGGAC 2 cut(s) 133, 392
BsoBI CYCGRG 1 cut(s) 40
Bsp143I GATC 6 cut(s) 76, 124, 273, 313, 463, 490
Bsp1720I GCTNAGC 1 cut(s) 590
BspACI CCGC 2 cut(s) 167, 178
BspCNI CTCAG 2 cut(s) 423, 582
BspDI ATCGAT 1 cut(s) 493
BspFNI CGCG 1 cut(s) 180
BspLI GGNNCC 1 cut(s) 502
BspMAI CTGCAG 2 cut(s) 273, 490
BsrDI GCAATG 2 cut(s) 25, 84
BssECI CCNNGG 1 cut(s) 450
BssMI GATC 6 cut(s) 76, 124, 273, 313, 463, 490
Bst4CI ACNGT 1 cut(s) 147
Bst6I CTCTTC 2 cut(s) 99, 134
BstC8I GCNNGC 1 cut(s) 131
BstDEI CTNAG 3 cut(s) 154, 431, 590
BstF5I GGATG 1 cut(s) 79
BstFNI CGCG 1 cut(s) 180
BstH2I RGCGCY 1 cut(s) 232
BstHHI GCGC 1 cut(s) 231
BstKTI GATC 6 cut(s) 79, 127, 276, 316, 466, 493
BstMAI GTCTC 2 cut(s) 136, 525
BstMBI GATC 6 cut(s) 76, 124, 273, 313, 463, 490
BstMWI GCNNNNNNNGC 2 cut(s) 245, 376
BstNSI RCATGY 1 cut(s) 350
BstSCI CCNGG 1 cut(s) 449
BstSFI CTRYAG 4 cut(s) 13, 269, 476, 486
BstUI CGCG 1 cut(s) 180
BstV1I GCAGC 4 cut(s) 66, 382, 472, 494
Bsu15I ATCGAT 1 cut(s) 493
Bsu36I CCTNAGG 1 cut(s) 431
BsuTUI ATCGAT 1 cut(s) 493
BtgZI GCGATG 1 cut(s) 297
BtrI CACGTC 1 cut(s) 202
BtsCI GGATG 1 cut(s) 79
Cac8I GCNNGC 1 cut(s) 131
CfoI GCGC 1 cut(s) 231
ClaI ATCGAT 1 cut(s) 493
CseI GACGC 1 cut(s) 432
CviAII CATG 4 cut(s) 62, 94, 224, 347
DdeI CTNAG 3 cut(s) 154, 431, 590
DpnI GATC 6 cut(s) 78, 126, 275, 315, 465, 492
DpnII GATC 6 cut(s) 76, 124, 273, 313, 463, 490
Eam1104I CTCTTC 2 cut(s) 99, 134
EarI CTCTTC 2 cut(s) 99, 134
Eco81I CCTNAGG 1 cut(s) 431
Eco88I CYCGRG 1 cut(s) 40
FaeI CATG 4 cut(s) 65, 97, 227, 350
FaiI YATR 9 cut(s) 63, 95, 225, 255, 348, 396, 405, 615, 627
FaqI GGGAC 2 cut(s) 133, 392
FatI CATG 4 cut(s) 61, 93, 223, 346
FauI CCCGC 1 cut(s) 185
FbaI TGATCA 1 cut(s) 76
Fnu4HI GCNGC 4 cut(s) 55, 371, 483, 486
FokI GGATG 1 cut(s) 86
Fsp4HI GCNGC 4 cut(s) 55, 371, 483, 486
FspBI CTAG 3 cut(s) 578, 648, 682
GlaI GCGC 1 cut(s) 230
GluI GCNGC 4 cut(s) 55, 371, 483, 486
HaeII RGCGCY 1 cut(s) 232
HapII CCGG 1 cut(s) 450
HgaI GACGC 1 cut(s) 432
HhaI GCGC 1 cut(s) 231
Hin1II CATG 4 cut(s) 65, 97, 227, 350
Hin6I GCGC 1 cut(s) 229
HinP1I GCGC 1 cut(s) 229
HindIII AAGCTT 1 cut(s) 399
HinfI GANTC 6 cut(s) 158, 163, 328, 495, 550, 568
HpaII CCGG 1 cut(s) 450
HphI GGTGA 2 cut(s) 373, 511
Hpy188I TCNGA 4 cut(s) 265, 327, 500, 645
Hpy188III TCNNGA 4 cut(s) 332, 409, 530, 547
HpyCH4III ACNGT 1 cut(s) 147
HpyCH4IV ACGT 2 cut(s) 201, 251
HpyCH4V TGCA 6 cut(s) 89, 271, 488, 610, 629, 635
HpyF10VI GCNNNNNNNGC 2 cut(s) 245, 376
HpyF3I CTNAG 3 cut(s) 154, 431, 590
HpySE526I ACGT 2 cut(s) 201, 251
Hsp92II CATG 4 cut(s) 65, 97, 227, 350
HspAI GCGC 1 cut(s) 229
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 6 cut(s) 76, 124, 273, 313, 463, 490
LmnI GCTCC 1 cut(s) 500
LpnPI CCDG 8 cut(s) 115, 210, 245, 344, 345, 443, 463, 464
Lsp1109I GCAGC 4 cut(s) 66, 382, 472, 494
LweI GCATC 2 cut(s) 199, 388
MaeI CTAG 3 cut(s) 578, 648, 682
MaeII ACGT 2 cut(s) 201, 251
MalI GATC 6 cut(s) 78, 126, 275, 315, 465, 492
MboI GATC 6 cut(s) 76, 124, 273, 313, 463, 490
MboII GAAGA 4 cut(s) 116, 151, 448, 545
MfeI CAATTG 1 cut(s) 317
MluCI AATT 2 cut(s) 289, 317
MmeI TCCRAC 1 cut(s) 668
MnlI CCTC 5 cut(s) 64, 100, 271, 426, 532
MseI TTAA 1 cut(s) 515
MslI CAYNNNNRTG 1 cut(s) 345
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 1 cut(s) 450
MspR9I CCNGG 1 cut(s) 451
MunI CAATTG 1 cut(s) 317
MvnI CGCG 1 cut(s) 180
MwoI GCNNNNNNNGC 2 cut(s) 245, 376
NciI CCSGG 1 cut(s) 451
NdeII GATC 6 cut(s) 76, 124, 273, 313, 463, 490
NlaIII CATG 4 cut(s) 65, 97, 227, 350
NlaIV GGNNCC 1 cut(s) 502
NspI RCATGY 1 cut(s) 350
PaeR7I CTCGAG 1 cut(s) 40
PfeI GAWTC 6 cut(s) 158, 163, 328, 495, 550, 568
PkrI GCNGC 4 cut(s) 56, 372, 484, 487
PsiI TTATAA 1 cut(s) 615
PspN4I GGNNCC 1 cut(s) 502
PspXI VCTCGAGB 1 cut(s) 40
PstI CTGCAG 2 cut(s) 273, 490
PvuII CAGCTG 1 cut(s) 485
RseI CAYNNNNRTG 1 cut(s) 345
SaqAI TTAA 1 cut(s) 515
SatI GCNGC 4 cut(s) 55, 371, 483, 486
Sau3AI GATC 6 cut(s) 76, 124, 273, 313, 463, 490
ScrFI CCNGG 1 cut(s) 451
SfaNI GCATC 2 cut(s) 199, 388
SfcI CTRYAG 4 cut(s) 13, 269, 476, 486
Sfr274I CTCGAG 1 cut(s) 40
SlaI CTCGAG 1 cut(s) 40
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 1 cut(s) 40
SmoI CTYRAG 1 cut(s) 40
Sse9I AATT 2 cut(s) 289, 317
SsiI CCGC 2 cut(s) 167, 178
SspMI CTAG 3 cut(s) 578, 648, 682
StyD4I CCNGG 1 cut(s) 449
TaaI ACNGT 1 cut(s) 147
TaiI ACGT 2 cut(s) 204, 254
TaqI TCGA 7 cut(s) 41, 161, 276, 301, 493, 529, 546
TasI AATT 2 cut(s) 289, 317
TfiI GAWTC 6 cut(s) 158, 163, 328, 495, 550, 568
Tru1I TTAA 1 cut(s) 515
Tru9I TTAA 1 cut(s) 515
TseI GCWGC 4 cut(s) 54, 370, 482, 485
TspDTI ATGAA 4 cut(s) 411, 428, 455, 532
XceI RCATGY 1 cut(s) 350
XhoI CTCGAG 1 cut(s) 40
XspI CTAG 3 cut(s) 578, 648, 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.